BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	67,471	A→T	P289P (CCT→CCA)	araA ←	L‑arabinose isomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	67,471	0	A	T	98.7%	469.4 / ‑3.9	154	P289P (CCT→CCA)	araA	L‑arabinose isomerase
Reads supporting (aligned to +/- strand): ref base A (2/0); new base T (69/83); total (71/83)
Fisher's exact test for biased strand distribution p-value = 2.11e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.99e-01

Only 100 of 154 total aligned reads displayed.

GAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTGCGGCGATCGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGACATCACCTTCATGATGCGAAGCAGGGCGGCAGTTTTCCAGTCGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGACCAGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGG‑TGAACGCGTGGAAGCCACCTTGTTCCAGGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTTGCCGTGGATTTGTGTGGCAGGCGTCATGGTGTAGCAGCTTTCGTACTCATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGGT  >  NC_000913/67225‑67716

gagaTGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAATCGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGACGCCCTGCAGACCGGTTGACATCACCTTCATGATGCGAAGCAGGGCGGCGGTTTTCCAGTCGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGACCTGGa                                                                                                                                                                                                                                                     >  1:952161/1‑250 (MQ=255)

 agaTGCTGAACGTCGCGGATCGGTTTCTCTTCTACGGCAATCGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGACATCACCTTCATGATGCGAAACAGGGCGGCGGTTTTCCAGTCGCCTTCCCCCGCACAGCCGTAACCCTGCTGCATCAGACGCTGTCCGGCCAGACCTGGaac                                                                                                                                                                                                                                                   >  1:183667/1‑250 (MQ=255)

       ggAACGTCGAGGATCGGTTTCTCTTCTACGGCAATCGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGACATCACCTTCATGATGCGAAGCAGGGCGGCGGTTTTCCAGTCGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGACCTGGAAGCTGttt                                                                                                                                                                                                                                             <  2:255667/250‑1 (MQ=255)

        gaactgcgtggaactgTTTCCCTTCTGCGGCCAGCGACGGGCAGCGTTCCAACACATGGTAGCTGAGCACCAGGTCATGTCTTTTCTCGATGTTATAGGGTTAGTCCGCCATAAAGGAGGTGCCGCCCTGCAGACCGTTTGTCATCACCGTCCTGATGCGAAGCAGGGCGGCGGTTTTCCAGTCGCCTTCGCCCGCAAAGCCGTATCCCTGCTTCAGCAGACTCTGTACGGCCAGTCCTGGAAGCTGttt                                                                                                                                                                                                                                             <  2:467742/235‑1 (MQ=255)

        gAACGTCGAGGATCGGTTTCTCTTCTACGGCAATCGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGACATCACCTTCATGATGCGAAGCAGGGCGGCGGTTTTCCAGTCGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGACCTGGAAGCTGTTTc                                                                                                                                                                                                                                            <  1:442615/251‑1 (MQ=255)

        gAACGTCGAGGATCGGTTTCTCGTCTACGGCAAGCGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGACATCACCTTCATGATGCGAAGCAGGGCGGCGGTTTTCCAGTCGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGACCTGGAAGCTGtt                                                                                                                                                                                                                                              <  1:934292/249‑1 (MQ=255)

         aaCGGCGAGGATCGGGTTCGTTTCTACGGCAATCGACGGGCAGACTTCCAGCATATGGGTGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGACATCACCTTCATGATGCGAAGCAGGGCGGCGGTTTTCCAGTCGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGACCTGGAAGCTGTTTCa                                                                                                                                                                                                                                           <  2:151791/251‑1 (MQ=255)

             ccGAGGATCGGTCTCTCTTCTACGGCAAGCGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGACATCACCTTCATGATGCGAAGCAGGGCGGCGGTTTTCCAGTCGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCATACCTGGAAGCTGTTTCAGAcc                                                                                                                                                                                                                                       <  2:704961/250‑1 (MQ=255)

              cGAGGATCGGTTTCTCTTCTACGGCAATCGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGACGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGACATCACCTTCATGATGCGAAGCAGGGCGGCGGTTTTCCAGTCGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCCGACGCTGTACGGCCAGACCTGGAAGCTGTTTCAGACCg                                                                                                                                                                                                                                      >  2:112799/1‑251 (MQ=255)

                  gATCGGTTTCTCTTCTACGGCAATCGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGACATCACCTTCATGATGCGAAGCAGGGCGGCGGTTTTCCAGTCGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGACCTGGAAGCTGTTTCAGACCg                                                                                                                                                                                                                                      >  1:99529/1‑247 (MQ=255)

                     cggtttctcccttacgtcaaccGACGGGCAGAATTCCAGCTAATGGGGACCGAGCCCCAGGTCATTTCCTTTCTCGAAGTGAGAGGTGTAGGCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGACATCACCTTCATTAGGCGAAGCAGGGCGGCGGTTTGCCAGTCGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGACCTGGAAGCTGTTTCAGACCGTGCaat                                                                                                                                                                                                                                <  2:756626/229‑2 (MQ=255)

                          tctcTTCTACGGCAATCGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGACATCACCTTCATGATGCGAAGCAGGGCGGCGGTTTTCCAGTCGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGACCTGGAAGCTGTTTCAGACCGTGCAAATCTTc                                                                                                                                                                                                                           <  1:963081/250‑1 (MQ=255)

                                  acggcaaccGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCTTGCAGACCGGTTGACATCACCTTCATGATGCGAAGCAGGGCGGCGGTTTTCCAGTCGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGACCTGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAggtgg                                                                                                                                                                                                                   <  2:863966/242‑1 (MQ=255)

                                    ggcaaTCGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGACATCACCTTCATGATGCGAAGCAGGGCGGCGGTTTTCCAGTCGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGACCTGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAggtggtgg                                                                                                                                                                                                                >  1:199045/5‑251 (MQ=255)

                                    ggcaaTCGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTATCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGACATCACCTTCATGATGCGAAGCAGGGCGGCGGTTTTCCAGTCGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGACCTGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAggtggtgg                                                                                                                                                                                                                <  1:503359/247‑1 (MQ=255)

                                       aaTCGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGACATCACCTTCATGATGCGAAGCAGGGCGGCGGTTTTCCAGTCGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGACCTGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGgcggtgg‑tg                                                                                                                                                                                                             >  1:526286/2‑250 (MQ=255)

                                           gACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGACATCACCTTCATGATGCGAAGCAGGGCGGCGGTTTTCCAGTCGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGACCTGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGG‑TGAAcgc                                                                                                                                                                                                        >  1:83198/1‑251 (MQ=255)

                                              gagCAGACTTCAAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGGGGTGCCGCCCTGCAGACCGGTTGACATCACCTTCATGATGCGATGCAGGGCGGCGGTTTTACAGTCGCCTTCGCCCGGAAAGCCGTAACCCTGCAGCATGAGACGCTGTACGGCCAGACCTGGCAGCTGCGTCAGAACGTGCGCATATTCAAAGGGGGTGG‑GGAACGCGTg                                                                                                                                                                                                     >  2:271043/3‑251 (MQ=255)

                                                 cataCTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGACATCACCTTCATGATGCGAAGCAGGGCGGCGGTTTTCCCGTCGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCCTCCGACGCTGTACGGCCAGACCTTGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTTG‑TGCACGCGTggc                                                                                                                                                                                                   >  2:164324/4‑249 (MQ=255)

                                                      ttCCAGCATATGGGCGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGACATCACCTTCATGATGCGAAGCAGGGCGGCGGTTTTCCAGTCGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGACCTGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGG‑TGAACGCGTGGAAGCCAc                                                                                                                                                                                             >  1:724561/1‑251 (MQ=255)

                                                        ccAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGACATCACCTTCATGATGCGAAGCAGGGCGGCGGTTTTCCAGTCGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGACCTGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGG‑TGAACGCGTGGAAGCCAcc                                                                                                                                                                                            <  1:135576/250‑1 (MQ=255)

                                                         cAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGACATCCCCTTCATGATGCGAAGCAGGGCGGCGGTTTTCCAGTCGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGACCTGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGG‑TGAACGCGTGGAAGCCACCtt                                                                                                                                                                                          <  1:601325/251‑1 (MQ=255)

                                                         cAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGACATCACCTTCATGATGCGAAGCAGGGCGGCGGTTTTCCAGTCGCCTTCACCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGACCTGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGG‑TGAACGCGTGGAAGCCACCtt                                                                                                                                                                                          <  1:837171/251‑1 (MQ=255)

                                                         cAGCATATGGGAGCCGAGCAACAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGACATCACCTTCATGATGCGAAGCCGGGCGGCGGTTTTCCAGTCGCCTTCGCCCGCAAAGCCGTAACACTGCTGCATCAGACGCTGTACGGCCAGACCTGGAAGCTGTTTCCGACCGTGCAAATCTTCAAAGGTGGTGG‑TGAACCCGTGGAAGCCACCtt                                                                                                                                                                                          >  2:224988/1‑251 (MQ=255)

                                                               atGGGAGCCGAGCTCCAGGTCATTTCCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGACATCACCTTCATGATGCGAAGCAGGGCGGCGGTTTTCCAGTCGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGTCAGACCTGGAAGCTGTTTCAGACCGTg                                                                                                                                                                                                                                    <  1:696183/204‑1 (MQ=255)

                                                               atGGGAGCCGAGCACCAGGTCATTACCTTTCTCGACGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGCCATCACCTTCATGATGCGAAGCAGGGCGGCGGTTTTCCAGTCGCCTTCGCCCGCCAAGCCGTACCCCTGCTGCATCAGACGCTGTACGGCCAGACCTGGCATCTGTTTCAGACCGTg                                                                                                                                                                                                                                    >  2:696183/1‑204 (MQ=255)

                                                               atGGGAGACGAGCTCCAGGTCATTACCTTTCTCGTAGTGATAGGTGGATTCCTCCATAAAGGAGATGCCGCCCTGCAGACCTGTTGACATCACCTTCAGGATGCGAAGCAGGGCGGCGGTTTTCCAGTCGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGACCTGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGG‑TGAACGCGTTTAAGCCCCCTTGTTcc                                                                                                                                                                                     <  2:551457/250‑1 (MQ=255)

                                                                  ggAGCCGAGCTCCAGGTCATTACCTTTCGCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGACATCACCTTCATGATGCGAAGCAGGGCGGCGGTTTTCCAGTCGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGACCTGGAAGCTGTTGCAGACCGGGCAAATCTTCAAAGGTGGTGG‑TGAACGCGTGGAAGCCACCTTGTTCCAGGa                                                                                                                                                                                 <  1:929622/251‑1 (MQ=255)

                                                                           cACCAGGTCGTTAACCTTCTCGCCGTGGTAGGTGTAGCACTACCTAAAGGCGGTGTCGCCCTGCAGACCGGTTGACATCACCTTCATGATGCGACGCAGGGCGGCGGATTTCCCGACCCCTTCGCCCGCAAAACCGCAACCCTGCTGCATCAGAGGCTGTACGGCCAGACCTGGAAGCTGTTTCAGACCAAGCAAATCTTCCAAGGAGGTGGGTGA‑CGGGAGGGAGCCACCGTGTTCCAGGAAAcactac                                                                                                                                                                         >  2:415047/1‑245 (MQ=255)

                                                                           cACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGACATCACCTTCATGATGCGAAGCAGGGCGGCGGTTTTCCAGTCGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGACCTGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGG‑TGAACGCGTGGAAGCCACCTTGTTCCAGGAAACGCTTc                                                                                                                                                                         >  1:39931/1‑250 (MQ=255)

                                                                             ccAGGGCATTACCTTTCTCGAAGTGATAGGGGTAGTCCTCCAGAAAGGAGGGGCCGCCCTGCAGACCGGTTGACATCACCTTCATGATGCGAAGCAGGGCGGCGGTTTTCCAGTCGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGACCTGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGG‑TGAACGCGTGGAAGCCACCTTGTTCCAGGAAACGCTTCATc                                                                                                                                                                      <  2:987401/251‑1 (MQ=255)

                                                                              cAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGACATCACCTTCATGATGCGAAGCAGGGCGGCGGTTTTCCAGTCGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGACCTGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGG‑TGAACGCGTGGAAGCCACCTTGTTCCAGGAAACGCTTCATcc                                                                                                                                                                     <  1:273251/251‑1 (MQ=255)

                                                                               aGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGACATCACCTTCATGATGCGAAGCAGGGCGGCGGTTTTCCAGTCGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGACCTGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGG‑TGAACGCGTGGAAGCCACCTTGTTCCAGGAAACGCTTCATccc                                                                                                                                                                    <  1:973090/251‑1 (MQ=255)

                                                                                ggTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGACATCACCTTCATGATGCGAAGCAGGGCGGCGGTTTTCCAGTCGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGACCTGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGG‑TGAACGCGTGGAAGCCACCTTGTTCCAGGAAACGCTTCATcccc                                                                                                                                                                   >  1:802507/1‑251 (MQ=255)

                                                                                   cATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGACATCACCTTCATGATGCGAAGCAGGGCGGCGGTTTTCCAGTCGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGACCTGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGG‑TGAACGCGTGGAAGCCACCTTGTTCCAGGACACGCTTCATCCCCAGc                                                                                                                                                                >  1:642492/1‑251 (MQ=255)

                                                                                        cctgttgcGAAGTGATAGTTGTTGTCCTCCATAAGGGAGGTGCCGCCCTGCAGACCGGTTGACCACACCTTCATGATGCGAAGCAGGGCGGCGGTTTTCCAGTCGCCTTCGCCCGCAAAGTCGTATCCCTGCTGCATCAGACGCTTTACGGCCAGCCCTGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTTG‑TGAACGCGTGGAAGCCACCTTGTTCCAGGAAACGCTTCATCCCAAGCTCAAt                                                                                                                                                           <  2:273665/244‑1 (MQ=255)

                                                                                               cGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGACATCACCTTCATGATGCGAAGCAGGGCGGCGGTTTTCCAGTCGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGACCTGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGG‑TGAACGCGTGGAAGCCACCTTGTTCCAGGAAACGCTTCATCCCCAGCTCAATAcgcg                                                                                                                                                      <  1:29398/249‑1 (MQ=255)

                                                                                               cGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGACATCACCTTCATGATGCGAAGCAGGGCGGCGGTTTTCCAGTCGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGACCTGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGG‑TGAACGCGTGGAAGCCACCTTGTTCCAGGAAACGCTTCATCCCCAGCTCAATAcgcg                                                                                                                                                      <  1:767148/249‑1 (MQ=255)

                                                                                                   gTGATAGGTGTAGTCCTCCATAAAGGAGGGGCCGCCCTGCAGACCGGTTGACATCACCTTCAGTATGCGAAGCAGGGCGGCGGTTTTCCAGTCGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGACCTGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGG‑TGAACGCGTGGAAGCCACCTTGTTCCAGGAAACGCTTCATCCCCAGCTCAATACGCGCCGCtt                                                                                                                                                <  2:83198/251‑1 (MQ=255)

                                                                                                        tggTGTAGTCCGCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGACATCACCTTCATGATGCGAAGCAGTGCGGCGGTGTTCCAGTCGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGACCTGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGG‑TGAACGCGTGGAAGCCACCTTGTTCCAGGAAACGCGTCATCTCCAGCTCAATACGCGCCGCTCCCAg                                                                                                                                            <  2:625742/249‑1 (MQ=255)

                                                                                                        aGGTGTAGTCCTCCATAAAGGAGGTGCCGGCCGGCAGACCGGTTGACATCACCTTCATGATGCGAAGCAGGGCGGCGGTGTTCCAGTCGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGGACGGCCAGACCTGGAAGCTGTTTCAGACCGTGCAAATCGTCAAAGGTGGTGG‑TGAACGCGGGGAAGCCACCTTGTTCCAGGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGc                                                                                                                                           <  1:155833/251‑1 (MQ=255)

                                                                                                           tgtAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGACATCACCTTCATGATGCGAAGCAGGGCGGCGGTTTTCCAGTCGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTATACGGCCAGACATGGAAGCTGTTTCAGCACGTGCAAATCTTCAAAGGTGGTGA‑TGCACGCGTGGAAGCCACCTTGTTCCAGGAAACACTTCATCCCCAGATCAATACGCGCCGCTTCCAGcccg                                                                                                                                        >  2:376296/1‑248 (MQ=255)

                                                                                                                    ccATAAAGGAGGAGCCGCCCTGCAGACCGGTTGACATCACCTTCATGATGCGAAGCAGGGCGGCGGTTTTCCAGTCGACTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGACCTGGAAGCTGTTTCAGACCGTGCAAATATTCAAAGGTGGTGG‑TGAACGCGTGGAAGCCACCTTGTTCCAGGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGttttaccgt                                                                                                                               >  2:14718/1‑244 (MQ=255)

                                                                                                                        aaaGGAGGTGCCGCCCTGCAGACCGGTTGACATCACCTTCATGATGCGAAGCAGGGCGGCGGTTTTCCAGTCGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGACCTGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGG‑TGAACGCGTGGAAGCCACCTTGTTCCAGGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGttttt                                                                                                                           <  1:90047/251‑1 (MQ=255)

                                                                                                                        aaaGGAGGTGCCGCCCTGCAGACCGGTTGACATCACCTTCATGATGCGAAGCAGGGCGGCGGTTTTCCAGTCGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGACCTGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGG‑TGAACGCGTGGAAGCCACCTTGTTCCAGGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGtttt                                                                                                                            <  1:878783/250‑1 (MQ=255)

                                                                                                                         aaGGAGGTGCCGCCCTGCAGACCGGTTGACATCACCTTCATGATGAGAAGCAGGGCGGCGGTTTTCCAGTCGCCTTCGCACGCAAAGCCGTAACCCGGCTGCATCAGACGCTGTACGGCCAGACCTGGAAGCTGTTTCAGACCGGGCAAATCTACAAAGGTGGTGG‑TGAACGCGTGGAAGCCACCTTGTGCCAGGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGtttttc                                                                                                                          <  1:651314/251‑2 (MQ=255)

                                                                                                                          aggaggTGCCGCCCTGCAGACCGGTTGACATCACCTTCATGATGCGAAGCAGGGCGGCGGTTTTCCAGTCGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGACCTGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGG‑TGAACGCGTGGAAGCCACCTTGTTCCAGGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGtttttcg                                                                                                                         <  1:566812/251‑3 (MQ=255)

                                                                                                                            gaggTGCCGCCCTGCAGACCGGTTGACATCACCTTCATGATGCGAAGCAGGGCGGCGGTTTTCCAGTCGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGACCTGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGG‑TGAACGCGTGGAAGCCACCTTGTTCCAGGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGcc                                                                                                                       >  1:171482/1‑251 (MQ=255)

                                                                                                                                 gccgccCTGCAGACCGGTTGACATCACCTTCATGATGCGAAGCAGGGCGGCGGTTTTCCAGTCGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGACCTGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGG‑TGAACGCGTGGAAGCCACCTTGTTCCAGGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCGTgg                                                                                                                   <  1:629833/250‑1 (MQ=255)

                                                                                                                                   cgccCTGCAGACCGGTTGACATCACCTTCATGATGCGAAGCAGGGCGGCGGTTTTCCAGTCGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGACCTGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGG‑TGAACGCGTGGAAGCCACCTTGTTCCAGGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCGTGGAtt                                                                                                                <  1:672308/251‑1 (MQ=255)

                                                                                                                                    gccCTGCAGACCGGTTGACATCACCTTCATGATGCGAAGCAGGGCGGCGGTTTTCCAGTCGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGACCTGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGG‑TGAACGCGTGGAAGCCACCTTGTTCCAGGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCGTGGAttt                                                                                                               >  1:515966/1‑251 (MQ=255)

                                                                                                                                      ccggccgACCGGTTGACATCCCCTGCATGATGCGAAGCAGGGCGGCGGTTTTCCCGTCGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCTTCAGACGCTGTACGGCCAGACCTGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGG‑TGAACGCGTGGAAGCCACCTTGTTCCAGGAAACGCGTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCGTGGATTtgt                                                                                                             <  2:319718/245‑1 (MQ=255)

                                                                                                                                      ccTGCAGACCGGTTGACATCACCTTCATGATGCGAAGCAGGGCGGCGGTTTTCCAGTCGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGACCTGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGG‑TGAACGCGTGGAAGCCACCTTGTTCCAGGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCGTGGATTtgt                                                                                                             <  1:467220/251‑1 (MQ=255)

                                                                                                                                       cTGCAGACCGGTTGACATCACCTTCATGATGCGAAGCAGGGCGGCGGTTTTCCAGTCGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGACCTGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGG‑TGAACGCGTGGAAGCCACCTTGTTCCAGGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCGTGGATTtgtg                                                                                                            >  1:476503/1‑251 (MQ=255)

                                                                                                                                         gCAGACCGGTTGACATCACCTTCATGATGCGAAGCAGGGCGGCGGTTTTCCAGTCGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGACCTGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGG‑TGAACGCGTGGAAGCCACCTTGTTCCAGGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCGTGGATTtgtgtt                                                                                                          <  1:205441/251‑2 (MQ=255)

                                                                                                                                             cccGGTTGGCATCACCTTCATGATGCGAAGCAGGGCGGCGGTTTTCCAGTCGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGACCTGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGG‑TGAACGCGTGGAAGCCACCTTGTTCCAGGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCGTGGATTTGTGTTGCAg                                                                                                      <  2:199045/250‑1 (MQ=255)

                                                                                                                                              ccGGTTGACATCACCTTCATGATGCGAAGCAGGGCGGCGGTTTTCCAGTCGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGACCTGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGG‑TGAACGCGTGGAAGCCACCTTGTTCCAGGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCGTGGATTTGTGTTGCAgg                                                                                                     >  1:33650/1‑251 (MQ=255)

                                                                                                                                              ccGGTTGACATCACCTTCATGATGCGAAGCAGGGCGGCGGTTTTCCAGTCGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGACCTGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGG‑TGAACGCGTGGAAGCCACCTTGTTCCAGGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCGTGGATTTGTGTTGCAgg                                                                                                     >  1:270297/1‑251 (MQ=255)

                                                                                                                                                 gTTGACATCACCTTCATGGTGCGAAGCAGGGCGGCGGTTTTCCAGTAGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGAACGGCCCAACCTGGAAGCTGTTTCAGACCGGGCAAATCTTCAAAGGGGGTGG‑TGAAAGCGGGGAAGACACCTTGTTCCAGGAAAAGCTTCATCCCCAGCTAAATACGCGCCGTTTCCAGCAAGTTCTGTCGtttttcgacgtagctttgtggtgacggcgt                                                                                                  >  2:934079/1‑226 (MQ=255)

                                                                                                                                                     aCATCACCTTCATGATGCGAAGCAGGGCGGCGGTTTTCCAGTCGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGACCTGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGG‑TGAACGCGTGGAAGCCACCTTGTTCCAGGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCGTGGATTTGTTTTGCAGGCGTCATg                                                                                              >  2:969890/1‑251 (MQ=255)

                                                                                                                                                        tCACCTTCATGATGCGAAGCAGGACGGCGGTTTTCCAGTCGCCTTAGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGACCTGGAAGATGTTTCAGACCGTGCAAATCTTCAAAGGGGGTGG‑TGAACGCGTGGAAGCCACCTTGTTCCCGGAAACGCTTCATCCCCCGCTCAATACGCGCGTCTTCCCGCACGTTCTGACGTTTCTCGCCGTGGTTTTGTGTTGCAGGCGTCATgggg                                                                                           >  2:910305/1‑249 (MQ=255)

                                                                                                                                                         cACCTTCATTATGCGAAGCAGGGCGGCGGTTTTCCAGTCGCCGTCGCCCGCAAAGCTGTAACCCTGCTGCATCAGACGCGTTACGGCCAGACCTGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGG‑TGAACGCGTGGAAGCCACCTTGTTCCAGGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCGTGGATTTGTGTTGCAGGCGTCATGgtgt                                                                                          <  2:544822/251‑1 (MQ=255)

                                                                                                                                                         cACCTTCATGATGTGAAGCAGGTCGGCGGTTTTCCAGGCGTCTTCGCCCGCAAAGCCGTAACCCTGCGTCATCAGGCGCTGTACGGCCAGACCTGGAAGCTGTTGCAGACCTTGCAAATCTGCAAAGGTGGTGG‑TGAACGCGTGGAAGCCACCTTGTTCCAGGAAACGCTTCATCCCCAGCGCAATCCGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCGTGGATTTGTGTTGCAGGCGTCATGgtgt                                                                                          <  2:870951/251‑1 (MQ=255)

                                                                                                                                                           ccTTCATGATGCGAAGCAGGGCGGCGGTTTTCCAGTCGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGATGTACGGACAGACCTGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGG‑TGAACGCGTTGAAGCCCCCGTGTTCCAGGAAACGCTTTATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCGTGGATTTGTGTTGCAGGCGTCATGGTGTag                                                                                        <  1:827402/251‑1 (MQ=255)

                                                                                                                                                              tCATGATGCGAAGCAGGGCGGCTGTTTTCCATTCGCCTGCGCCCGCAAAGCCGTACCCCTGCTGCATCAGACGCTGTACGGCCAGACCTGGAAGCTGGTTCAGACCGTGCAAATCTTCAAAGGTGGTGG‑TTAACGCGTGGAAGCCACCTTGTTCCAGGAAACGCTTCATCCCCAGTTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCGTGGATTTGTGTTGCAGGCGGCATTGTGTagca                                                                                      <  2:389155/250‑1 (MQ=255)

                                                                                                                                                              tCATGATGCGAAGCAGGGCGGCGGTTTTCCAGGCGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGACCTGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGG‑TGAACGCGTGGAAGCCACCTTGTTCCAGGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTGCTGTCGTTTTTCGCCGTGGATTTGTGTTGCAGGCGTCATGGTGTagca                                                                                      <  2:181715/250‑1 (MQ=255)

                                                                                                                                                                atgatgCGAAGCAGGGCGGCGGTTTTCCAGTCGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGACCTGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGG‑TGAACGCGTGGAAGCCACCTTGTTCCAGGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCGTGGATTTGTGTTGCAGGCGTCATGGTGTAGCAGCt                                                                                   <  1:479146/251‑1 (MQ=255)

                                                                                                                                                                  gatgCGAAGCAGGGCGGCGGTTTTCCAGTCGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGACCTGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGG‑TGAACGCGTGGAAGCCACCTTGTTCCAGGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCGGGGCATTGTGTTGCAGGCGTCATGGTGTAGCAGCttt                                                                                 >  2:820275/1‑251 (MQ=255)

                                                                                                                                                                   atgCGAAGCAGGGCGGCGGTTTTCCAGTCGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGACCTGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGG‑TGAACGCGTGGAAGACACCTTGTTCCAGGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCGTGGATTTGTGTTGCAGGCGTCATGGTGTAGCAGCTTTc                                                                                >  1:625383/1‑251 (MQ=255)

                                                                                                                                                                           cAGGGCGGCGGTTTTCCAGTCGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGACCTGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGG‑TGAACGCGTGGAAGCCACCTTGTTCCAGGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCGTGGATTTGTGTTGCAGGCGTCATGGTGTAGCAGCTTTCGTACTCAt                                                                        <  2:486413/251‑1 (MQ=255)

                                                                                                                                                                            aGGGCGGCGGTTTTCCAGTCGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGACCTGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGG‑TGAACGCGTg                                                                                                                                                                                                     <  2:553778/125‑1 (MQ=255)

                                                                                                                                                                            aGGGCGGCGGTTTTCCAGTCGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGACCTGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGG‑TGAACGCGTg                                                                                                                                                                                                     >  1:553778/1‑125 (MQ=255)

                                                                                                                                                                             gggCGGCGGTTTTCCAGTCGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGACCTGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGG‑TGAACGCGTGGAAGCCACCTTGTTCCAGGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCGTGGATTTGTGTTGCAGGCGTCATGGTGTAGCAGCTTTCGTACTCATCg                                                                      <  1:607793/251‑1 (MQ=255)

                                                                                                                                                                               gtggcggttgtCCAGTCCCCGTCGCCCGCAAAGCCGGAACCCTGCTGCATCAGACGGTGTACGGCTAGACCTTGAAGCGGTTTCAGACCGAGCAAATCTTCAAAGGTGGTGG‑TTGACGCGTGGAAGCCACCTTGTTCCAGAAAACGCTTCATCCCCAGCTCAATACGCGCCGCTACCGGCACGTTCTGTCGTGTTTCTCCTTGGATGTGTGTTGCAGTCGTCATGGGGTAGCAGCTTTCGTATTCATTGAc                                                                    <  2:659178/241‑1 (MQ=255)

                                                                                                                                                                                 ggcggTTTGCCAGGCGTCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGACCTGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGG‑TGAACGCGTGGAAGCCACCTTGTTCCAGGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCGTGGATTTGTGTTGCAGGCGTCATGGTGTAGCAGCTTTCGGACTCATCGACCa                                                                  <  1:250635/247‑1 (MQ=255)

                                                                                                                                                                                 ggcggTTTGCCAGGCGCCTTCGCCCGCAAAGCCGTAACCCTTCTGCAACAGACGCTGTACGGCCAGACCTGGAAGCTGTTTCAGACCGTGCAAATCTTCCAAGGTGGTTG‑TGAACGCGGGGAAGCCACCTAGTTCCAGGAAACGCTTCATTCCCAGCTCAATACTCTCCGCTTCCAGCACGTTCTGTCGGTTTTCGCCTTGGATTTGTGTTGCATGCTTCATTGTGTAGCAGCATTCGTACTCATCGAcct                                                                  <  2:361458/247‑2 (MQ=255)

                                                                                                                                                                                   aggTTTTCCAGTCGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGACCTGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGG‑TGAACGCGTGGACGCCACCTTGTTCCAGGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCGTGGATTTGTGTTGCAGGCGTCATGGTATAGCCGCTTTCGTACTCATCGACCAgc                                                                >  2:669066/3‑251 (MQ=255)

                                                                                                                                                                                       tttCCAGTCGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGACCTGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGG‑TGAACGCGTGGAAGCCACCTTGTTCCAGGTAACGCTTCATCCCCAGCTCAATACGCTCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCGTGGATTTGTGTTGCAGGCGGCATGGTGTAGCCGCTTTCGTACTACTCGACCAGCGCGt                                                            >  2:788888/1‑251 (MQ=255)

                                                                                                                                                                                       tttCCAGTCGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGACCTGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGG‑TGAACGCGTGGAAGCCACCTTGTTCCAGGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCGTGGATTTGTGTTGCAGGCGTCATGGTGTAGCAGCTTTCGTACTCATCGACCAGCGCGt                                                            >  1:425255/1‑251 (MQ=255)

                                                                                                                                                                                       tttCCAGTCGCCTACGCCCGCAAAGCCGTAAACCTGCTGCATCAGACGCTGTACGGCCAGACCTGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGG‑TGAACGCGTGGAAGCCACCTTGTTCCAGGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCGTGGATTTGTGTTGCAGGCGTCATGGTGTAGCAGCTTTCGTACTCATCGACCAGCGCGt                                                            >  1:270864/1‑251 (MQ=255)

                                                                                                                                                                                         tCCAGTCGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCCTCAGACGCTGTACGGCCAGACCTGGAAGCTGTTTCAGGCCGTGCAAATCTTCAAAGGTGGTGG‑TGAACGCGTGGAAGCCACCTTGTTCCAGGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCAGTGGATTTGTGTTGCAGGCGTCATGGTGTAGCAGCTTTCGTACTCATCGActatcgagtt                                                           >  1:950063/1‑241 (MQ=255)

                                                                                                                                                                                          ccAGTCGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGCCCTGTAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGG‑TGAACGCGTGGAAGCCAACTTGTTCCAGGAAACGCTTCATCCCCAGCTCATTACGCGCCGCTTCCAGCACGTTATGTCGTTTTTCTCCGTCGATTTGTGTTGCTGGCGTCATGTTGTAGCAGCTTTCTGACTCATAGACCAGCGCGTTa                                                          >  2:400089/1‑250 (MQ=255)

                                                                                                                                                                                           ccgTCGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTGCGGCCAGACCTGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGG‑TGAACGCGTGGAAGCCACCTTGTTCCAGGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTTTGTCGTTTTTCGCCGTGGATTTGTGTTGCAGGCGTCATGGTGTAGCAGCTTTCGTACTCATCGACCAGCGCGTTAAc                                                        <  2:703611/249‑1 (MQ=255)

                                                                                                                                                                                               cGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGACCTGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGG‑TGAACGCGTGGAAGCCACCTTGTTCCAGGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCGTGGATTTGTGTTGCAGGCGTCATGGTGTAGCAGCTTTCGTACTCATCGACCAGCGCGTTAACATCg                                                    <  1:939084/251‑1 (MQ=255)

                                                                                                                                                                                                gCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGACCTGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGG‑TGAACGCGTGGAAGCCACCTTGTTCCAGGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCGTGGATTTGTGTTGCAGGCGTCATGGTGTAGCAGCTTTc                                                                                <  1:664714/222‑1 (MQ=255)

                                                                                                                                                                                                 ccTTAGCCCGCAAAGCCGGAACCCTGCTGCATCAGACGCTGTACGGCAAGACATGGAAGCTGTGTCAGACCGGGCAAATCTTCAAAGGTGGTGG‑TGAACGCGTGGAAGCCACCTTGTTCCAGGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCGTGGATTTGTGTTGCAGGCGTCATGGTGTAGCAGCTTTCGTACTCATCGACCAGCGCGTTAACATCGct                                                  <  2:249732/251‑2 (MQ=255)

                                                                                                                                                                                                   ttCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGCCCTGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGG‑TGAACGCGTGGAAGCCACCTTGTTCCAGGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCGTGGATTTGTGTTGCAGGCGTCATGGTGTAGCAGCTTTCGTACTCCTCGACCAGCCCGTTAACATCGCCGt                                                >  2:263194/1‑251 (MQ=255)

                                                                                                                                                                                                    tCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGACCTGGAAGCTGTTGCAGACCGTGCAAATCTTCAAAGGTGGTGG‑TGAACGCGTGGAAGCCACCTTGTTCCAGGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTGCCAGCACGTTCTGTCGTTTTTCGCCGTGGATTTGTGTTGCAGGCGTCATGGTGTAGCAGCTTTCGTACTCATCGACCAGCGCGTTAACATCGCCg                                                 <  2:327982/249‑1 (MQ=255)

                                                                                                                                                                                                          gCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGACCTGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGG‑TGAACGCGTGGAACCCCCCTTGTTCCAGGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCGTGGATTTGTGTTGCAGGCGTCATGGTGTAGCAGCTTTCGTACTCATCGACCAGCGCGTTACCATCGCCGTCGCTGAt                                         >  1:954250/1‑251 (MQ=255)

                                                                                                                                                                                                            aaaGCCGTATCCCTGCTTCATCAGACGCTGTCCGGCCAGTCCTGTAAGCGGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGG‑TGAACGCGTGGAAGCCTCCTTGTTCCAGGAAACGCTTCATCCCCAGCGCAATACGCTCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCGTGGATTTGTGTTGCAGGCGTCATGGTGTAGCAGCTTTCGTACGCATCGACCAGCGCGTTAACATCGCCGTCGCTGATgg                                       <  2:571504/251‑1 (MQ=255)

                                                                                                                                                                                                               gCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGACCTGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGG‑TGAACGCGTGGAAGCCACCTTGTTCCAGGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCGTGGATTTGTGTTGCAGGCGTCATGGTGTAGCAGCTTTCGTACTCATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGt                                    >  1:285940/1‑251 (MQ=255)

                                                                                                                                                                                                                ccGTAACCCTGCTGCATCAGACGATGTACAGCCAGACCTGGAAGCTGTTTCCGACCGTGCAAATCTTCAAAGGTGGTGG‑TGAACGCGTGGAAGCCACCTTGTTCCAGGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTGGTTCTTCGCCGTGGATTTGTGGTGCAGGCGTCATGGTGTAGCAGCTTTCGTACTCATCGACCAGCGCGTTAACATCGCCggggctgagtgggtt                                   >  2:988942/1‑237 (MQ=255)

                                                                                                                                                                                                                    aaCCCTGCTGCATCAGACGCTGATCGGCCAGACCTGGAAGCTGTTTCAGACCGTGCCAATCTTGAAAGGTGGTGG‑TGAACGCGTGGAAGCCACCTTGTTCCAGGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGGCGTTTTTCGCCGTGGATTTGTGTTGCAGGCGTCATGGTGTAGCAGCGTTCGTACTCATCGACCAGCGCGTTAACATCGCCGTCGCTGAGGGAGTTcac                                <  2:891850/250‑1 (MQ=255)

                                                                                                                                                                                                                       ccTGCTGCATCAGACGCTGTACGGCCAGACCTGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGG‑TGAACGCGTGGAAGCCACCTTGTTCCAGGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCGTGGCTTTGTGTTGCAGGCGTCATGGTGTAGCAGCTTTCGTACTCATCGGCCCGCGCGTTAACATCGCCGTCGCTGTTGGGTTTCaccacc                            >  2:483391/1‑251 (MQ=255)

                                                                                                                                                                                                                             gcATCAGACGCTGTACGGCCAGACCAGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGG‑TGAACGCGTGGAAGCCACCTTGTTCCAGGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCGTGGATTTGTGTTGCAGGCGTCATGGTGTAGCAGCTTTCGTACTCATCGACCACCGCGTTAACATCGCCTTCGCTGATGGAGTTCACCACCTGCAcc                      >  2:170986/1‑251 (MQ=255)

                                                                                                                                                                                                                              cATCAGACGCTGTACGGCCAGACCTGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGG‑TGAACGCGTGGAAGCCACCTTGTTCCAGTAAACGCTTCATCGCCATCTCAATACTCGCCGCGTCCAGCACGTTCTGTCGTTTTTCGCCGTGGATTTGTGTTGCGGGCGTCATGGTGTAGCAGCTTTTGTACTCCTCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCa                     <  1:474669/251‑1 (MQ=255)

                                                                                                                                                                                                                                tCAGACGCTGTACGGCCAGACCTGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGG‑TGAACGCGTGGAAGCCACCTTGTTCCAGGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCGTGGATTTGTGTTGCAGGCGTCATGGTGTAGCAGCTTTCGTACTCATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCAcc                      >  2:297267/1‑248 (MQ=255)

                                                                                                                                                                                                                                   gACGCTGTACGGCCAGACCTGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGG‑TGAACGCGTGGAAGCCACCTTGTTCCAGGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCGTGGATTTGTGTTGCAGGCGTCATGGTGTAGCAGCTTTCGTACTCATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCg                <  1:592336/251‑1 (MQ=255)

                                                                                                                                                                                                                                   gACGCTGTACGGCCAGACCTGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGG‑TGAACGCGTGGAAGCCACCTTGTTCCAGGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCGTGGATTTGTGTTGCAGGCGTCATGGTGTAGCAGCTTTCGTACTCATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCg                <  1:246271/251‑1 (MQ=255)

                                                                                                                                                                                                                                     cGCTGTACGGCCAGACCTGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGG‑TGAACGCGTGGAAGCCACCTTGTTCCAGGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCGTGGATTTGTGTTGCAGGCGTCATGGTGTAGCAGCTTTCGTACTCATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGcc              >  1:613907/1‑251 (MQ=255)

GAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTGCGGCGATCGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGACATCACCTTCATGATGCGAAGCAGGGCGGCAGTTTTCCAGTCGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGACCAGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGG‑TGAACGCGTGGAAGCCACCTTGTTCCAGGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTTGCCGTGGATTTGTGTGGCAGGCGTCATGGTGTAGCAGCTTTCGTACTCATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGGT  >  NC_000913/67225‑67716

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`