BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	67,264	G→A	I358I (ATC→ATT)	araA ←	L‑arabinose isomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	67,264	0	G	A	97.5%	496.2 / 0.6	161	I358I (ATC→ATT)	araA	L‑arabinose isomerase
Reads supporting (aligned to +/- strand): ref base G (2/2); new base A (80/75); total (83/78)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.98e-01

Only 100 of 163 total aligned reads displayed.

CGGAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATGCAGTTAACCAGTAGACGGTAACGATCGCCGAGATCAATCAAGCTGGCGACAATCGCTGGGCCGGTTTGGGTATTGAAGATCAGGCGGGCAG‑GATCGTCCTTACCACCAATACCGAG‑ATGCTGAACGTCGAGGATCGGTTTCTCTTCTGCGGCGAT‑CGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGACATCACCTTCATGATGCGAAGCAGGGCGGCAGTTTTCCAGTCGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGACCAGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGT  >  NC_000913/67020‑67509

cGGAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAG‑GATCGTCCTTACCACCAATACCGAG‑ATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAAT‑CGAc                                                                                                                                                                                                                                                 >  1:987401/1‑251 (MQ=255)

cGGAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAG‑GATCGTCCTTACCACCAATACCGAG‑ATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAAT‑CGAc                                                                                                                                                                                                                                                 <  1:5616/251‑1 (MQ=255)

 ggAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCTCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAG‑GATCGTCCTTACCACCAATACCGAG‑ATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAAT‑CGACg                                                                                                                                                                                                                                                <  1:62/251‑1 (MQ=255)

  gAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGTGCAG‑GATCGTCCTTACCACCAATACCGAG‑ATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAAT‑CGACgg                                                                                                                                                                                                                                               <  1:100231/251‑1 (MQ=255)

      cAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAG‑GATCGTCCTTACCACCAATACCGAG‑ATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAAT‑CGACgagc                                                                                                                                                                                                                                             >  1:480552/1‑246 (MQ=255)

       aGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTTTCGATGCAGTTAACCAGTAGACGGTAACGGTCGCCGAGATCCATCCAGCTGGCGACAATCGCTGGGCCGGTTTGGGTATTTAAGATCCGGCGGG‑AGAGGTCGTCCTTACCCCCAATCCCGAGCAT‑CTGCACGTCGAGGGTCTGTTTCTCTTCTtcggcggaccaccggc                                                                                                                                                                                                                                              >  2:81136/1‑238 (MQ=255)

        gTTGGCAGATCCGGTTGCGCGTTCCACAGCGCAGTCGCCACCGGCATTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAG‑GATCGTCCTTACCACCAATACCGAG‑ATGCTGAACGTCGAGGAGCGGTTTCTCTTCTACGGCAAT‑CGACGGGCAGAc                                                                                                                                                                                                                                         <  2:629216/251‑1 (MQ=255)

           ggCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAG‑GATCGTCCTTACCACCAATACCGAG‑ATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAAT‑CGACGGGCAGACTTc                                                                                                                                                                                                                                      >  1:291182/1‑251 (MQ=255)

             cAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCCGTTTCGGCAGGGTGTGCGGTGTTTTAACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGCGATCAATCAGGCTGGCGACAATCCCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAG‑GATCGTCCTTACCACCAATACCGAG‑ATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAAT‑CGACCGGCCGGCTTccc                                                                                                                                                                                                                                    >  2:442615/1‑250 (MQ=255)

                 tCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAG‑GATCGTCCTTACCACCAATACCGAG‑ATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAAT‑CGACGGGCAGACTTCCAGCat                                                                                                                                                                                                                                >  1:371861/1‑251 (MQ=255)

                  ccGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAG‑GATCGTCCTTACCACCAATACCGAG‑ATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAAT‑CGACGGGCAGACTTCCAGCata                                                                                                                                                                                                                               <  1:245260/251‑1 (MQ=255)

                    ggTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCCCCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAG‑GATCGTCCTTACCACCCATACCGAG‑ATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGG‑AATACGACGGGCCGACTTCCCGCATATg                                                                                                                                                                                                                             >  2:434601/1‑251 (MQ=255)

                    ggTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAG‑GATCGTCCTTACCACCAATACCGAG‑ATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAAT‑CGACGGGCAGACTTCCAGCATATg                                                                                                                                                                                                                             >  1:255667/1‑251 (MQ=255)

                      ttGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAG‑GATCGTCCTTACCACCAATACCGAG‑ATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAAT‑CGACGGGCAGACTTCCAGCATATggg                                                                                                                                                                                                                           >  1:103894/1‑251 (MQ=255)

                       tGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCCGTCGGGCAG‑GATCGTCCTTACCACCAATACCGAG‑ATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAAT‑CGACGGGCAGACTTCCTGCATATggg                                                                                                                                                                                                                           >  1:407969/1‑250 (MQ=255)

                       tGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAG‑GATCGTCCTTACCACCAATACCGAG‑ATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAAT‑CGACGGGCAGACTTCCAGCATATggg                                                                                                                                                                                                                           <  1:752694/250‑1 (MQ=255)

                                 acaGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAG‑GATCGTCCTTACCACCAATACCGAG‑ATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAAT‑CGACGGGCAGACTTCCAGCATATGGGAGCCGAGCAcc                                                                                                                                                                                                                <  1:339706/251‑1 (MQ=255)

                                      gcATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGGGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGGTCAATCAGGCTGGGGACAAACGCTGTCCCGGGTTGGGTATTGAAGCACAGTCGGGCAG‑GATCGTCCTTACCACCAATACCGAG‑GTGCTGTACGGCGAGGAGCGGTTTCTCTTCTACGTCAAT‑CGACGGGCCGACTTCCAGCATATGGGTGTCGAGCACCAgggc                                                                                                                                                                                                           >  2:246271/1‑249 (MQ=255)

                                          tCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAG‑GATCGTCCTTACCACCAATACCGAG‑ATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAAT‑CGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCAtt                                                                                                                                                                                                        <  1:55582/250‑1 (MQ=255)

                                           cGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAG‑GATCGTCCTTACCACCAATACCGAG‑ATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAAT‑CGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTa                                                                                                                                                                                                       <  2:475848/250‑1 (MQ=255)

                                             ccACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAG‑GATCGTCCTTACCACCAATACCGAG‑ATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAAT‑CGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCt                                                                                                                                                                                                    <  1:274558/251‑1 (MQ=255)

                                                  ggCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCCGTCGGGCCG‑GATCGTCCTTACCACCAATACCGAG‑ATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAAT‑CGACGGGCAGACTTCCCGCATATGGGGGCCGAGCACCGGGTCCTTACCTTtctc                                                                                                                                                                                               >  2:876194/1‑251 (MQ=255)

                                                    cAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAG‑GATCGTCCTTACCACCAATACCGAG‑ATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAAT‑CGACGGGCAGACTTCCAGCATATGGGAGCCGCGCACCAGGTCATTACCTTTCTCg                                                                                                                                                                                              >  1:703611/1‑250 (MQ=255)

                                                     aGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCAGTTTGGGTATTGAAGATCAGTCGGGCAG‑GATCGTCCTTACCCCCAATACCGAG‑ATGCTGAACGACGAGGATCGGTTTCTCTTCTACGGCAAT‑CGACGGGCAGACTTCCCGCATATGGGAGCCCAGCACCAGGGCATTCCCTTTCTCgc                                                                                                                                                                                             >  2:90047/1‑249 (MQ=255)

                                                      gTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAG‑GATCGTCCTTACCACCAATACCGAG‑ATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAAT‑CGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGa                                                                                                                                                                                             >  1:486413/1‑249 (MQ=255)

                                                                  aGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAG‑GATCGTCCTTACCACCAATACCGAG‑ATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAAT‑CGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAg                                                                                                                                                                               <  1:520113/251‑1 (MQ=255)

                                                                    tGCGGTGTTTTCACCGTGTCGATACAGTTAACAAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAG‑GATCGTCCTTACCACCAATACCGAG‑ATGCTGAACGTCGAGGAACGGTTTCTCTTCTACGGCAAT‑CGACGGGCAGACTCCCAGCATATGGGAGCCGACCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGtc                                                                                                                                                                             >  2:963392/1‑251 (MQ=255)

                                                                        gtgtttgacccgtttcgagacAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAG‑GATCGTCCTTACCACCAATACCGAG‑ATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAAT‑CGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGtcctcc                                                                                                                                                                         <  2:931356/231‑1 (MQ=255)

                                                                          gtTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAG‑GATCGTCCTTACCACCAATACCGAG‑ATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAAT‑CGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGACATTACCTTTCTCGAAGGGATAGGTGTAGTCCTCCa                                                                                                                                                                        >  1:707645/1‑250 (MQ=255)

                                                                                 ccGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAG‑GATCGTCCTTACCACCAATACCGAG‑ATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAAT‑CGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAAGTCATTACCTTTCTCGAAGGGATAGGTGTAGTCCTCCATAAagg                                                                                                                                                                  >  2:837171/1‑249 (MQ=255)

                                                                                  cGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAG‑GATCGTCCTTACCACCAATACCGAG‑ATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAAT‑CGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAATCCTCCCTAAaggagg                                                                                                                                                               >  2:773408/1‑251 (MQ=255)

                                                                                    tgtCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAG‑GATCGTCCTTACCACCAATACCGAG‑ATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAAT‑CGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGCGGTg                                                                                                                                                             >  1:97405/1‑251 (MQ=255)

                                                                                     gtCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAG‑GATCGTCCTTACCACCAATACCGAG‑ATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAAT‑CGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGCGTAGGCCTCCATAAAGGAGGTgc                                                                                                                                                            >  1:123366/1‑251 (MQ=255)

                                                                                      tCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAG‑GATCGTCCTTACCACCAATACCGAG‑ATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAAT‑CGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGGGATAGGTGTAGTCCTCCATAAAGGAGGTgcc                                                                                                                                                           >  2:878783/1‑251 (MQ=255)

                                                                                               ttAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAG‑GATCGTCCTTACCACCAATACCGAG‑ATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAAT‑CGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGc                                                                                                                                                    >  1:178277/1‑249 (MQ=255)

                                                                                                tAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAG‑GATCGTCCTTACCACCAATACCGAG‑ATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAAT‑CGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGTTGCCGCCCTGCAg                                                                                                                                                  <  1:758493/250‑1 (MQ=255)

                                                                                                tAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAG‑GATCGTCCTTACCACCAATACCGAG‑ATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAAT‑CGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAg                                                                                                                                                  <  1:572939/250‑1 (MQ=255)

                                                                                                   ccAGCAGATGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAG‑GATCGTCCTTACCACCAATACCGAG‑ATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAAT‑CGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCg                                                                                                                                              <  1:512931/251‑1 (MQ=255)

                                                                                                    cagcaGACGGTAACGATCGCCGAGAGCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAG‑GATCGTCCTTACCACCAATACCGAG‑ATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAAT‑CGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCgg                                                                                                                                             <  2:599797/247‑1 (MQ=255)

                                                                                                    caacatgagggaacagtcgcatagaTCAATCAGGCTGGCGCAAATCGCTGGATCGGTTTGGGTATTGAAGATCAGTCTGGCAG‑GATCGTCCTTACCACCAATACCGAG‑ATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAAT‑CGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGAGAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCgg                                                                                                                                             <  2:300319/229‑1 (MQ=255)

                                                                                                     agcaGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAG‑GATCGTCCTTACCACCAATACCGAG‑ATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAAT‑CGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGt                                                                                                                                            <  1:434601/248‑1 (MQ=255)

                                                                                                      gcaGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAG‑GATCGTCCTTACCACCAATACCGAG‑ATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAAT‑CGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGtt                                                                                                                                           >  1:165893/3‑251 (MQ=255)

                                                                                                         gACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAG‑GATCGTCCTTACCACCAATACCGAG‑ATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAAT‑CGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGAc                                                                                                                                        <  1:310691/251‑1 (MQ=255)

                                                                                                            ggTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAG‑GATCGTCCTTACCACCAATACCGAG‑ATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAAT‑CGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGACATc                                                                                                                                     >  1:281574/1‑251 (MQ=255)

                                                                                                             gTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAG‑GATCGTCCTTACCACCAATACCGAG‑ATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAAT‑CGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGACATCa                                                                                                                                    <  1:695448/251‑1 (MQ=255)

                                                                                                               aaCGATCGCCGAGAGCAATCAGGCGGGCGACAAATGCTGGACAGGTTTGGGTAATGAAGATCAGTCGGGCAG‑GATCGTCCTTACCACCAATACCGAG‑ATGCTGAACGGCGAGGATCGGGTTCTCTTCTACGGCAAT‑CGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGAAATCAc                                                                                                                                   <  2:349276/250‑1 (MQ=255)

                                                                                                                aCGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAG‑GATCGTCCTTACCACCAATACCGAG‑ATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAAT‑CGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGACATCACCt                                                                                                                                 <  2:172407/251‑1 (MQ=255)

                                                                                                                   aTCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAG‑GATCGTCCTTTCCACCAATACCGAG‑ATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAAT‑CGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGACATCACCTTCa                                                                                                                              <  1:947662/251‑1 (MQ=255)

                                                                                                                        cGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAG‑GATCGTCCTTACCACCAATACCGAG‑ATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAAT‑CGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGACATCACCTTCatgatg                                                                                                                         >  1:327982/1‑251 (MQ=255)

                                                                                                                          agaTCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAG‑GATCGTCCTTACCACCAATACCGAG‑ATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAAT‑CGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGACATCACCTTCATGATGCg                                                                                                                       <  1:175604/251‑1 (MQ=255)

                                                                                                                           gaTCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAG‑GATCGTCCTTACCACCAATACCGAG‑ATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAAT‑CGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGACATCACCTTCATGATGCGa                                                                                                                      <  1:902378/251‑1 (MQ=255)

                                                                                                                                  caggCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAG‑GATCGTCCTTACCACCAATACCGAG‑ATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAAT‑CGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGACATCACCTTCATGATGCGAAGCAggg                                                                                                               <  2:702692/248‑1 (MQ=255)

                                                                                                                                      cTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAG‑GATCGTCCTTACCACCAATACCGAG‑ATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAAT‑CGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGACATCACCTTCATGATGCGAAGCAGggcggc                                                                                                           >  2:601325/1‑251 (MQ=255)

                                                                                                                                       gggCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAG‑GATCGTCCTTACCACCAATACCGAG‑ATGCTGAACGTCGAGGAGCGGTTTCTCTTCTACGGCAAT‑CGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGACATCACCTTCATGATGCGAAGCAGggcggcg                                                                                                          <  2:955330/250‑2 (MQ=255)

                                                                                                                                        ggCGACAATCGCTGGACCTGTTTGGGTATTGAAGATCAGTCGGGCAG‑GATCGTCCTTACCACCAATACCGAG‑ATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAAT‑CGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGACATCACCTTCATGATGCGAAGCAGggcggc                                                                                                           <  1:213804/249‑1 (MQ=255)

                                                                                                                                        ggCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAG‑GATCGTCCTTACCACCAATACCGAG‑ATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAAT‑CGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGACATCACCTTCATGCTGCGAAGCAGagcggcgg                                                                                                         >  1:645640/1‑249 (MQ=255)

                                                                                                                                         gCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAG‑GATCGTCCTTACCACCAATACCGAG‑ATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAAT‑CGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGACATCACCTTCATGATGCGAAGCAGggcggcggt                                                                                                        >  1:319718/1‑248 (MQ=255)

                                                                                                                                          cGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAG‑GATCGTCCTTACCACCAATACCGAG‑ATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAAT‑CGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGTTAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGACATCACCTTCATGATGCGAAGCAGggcggcgggt                                                                                                       >  2:427829/1‑247 (MQ=255)

                                                                                                                                            aCAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAG‑GATCGTCCTTACCACCAATACCGAG‑ATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAAT‑CGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGACATCACCTTCATGATGCGAAGCAGGGCGGCGGtttt                                                                                                     <  2:291182/251‑1 (MQ=255)

                                                                                                                                            aCAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAG‑GATCGTCCTTACCACCAATACCGAG‑ATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAAT‑CGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGACATCACCTTCATGATGCGAAGCAGGGCGGCGGtttt                                                                                                     <  1:856076/251‑1 (MQ=255)

                                                                                                                                                      gaccGGTTTGGGTATTGAAGATCAGTCGGGCAG‑GATCGTCCTTACCACCAATACCGAG‑ATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAAT‑CGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGACATCACCTTCATGATGCGAAGCAGGGCGGCGGTTTTCCAGTCGCCt                                                                                           >  1:140869/3‑251 (MQ=255)

                                                                                                                                                        ccGGTTTGGGTATTGAAGATCAGTCGGGCAG‑GATCGTCCTTACCACCAATACCGAG‑ATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAAT‑CGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCCGGTCATTACCTTTCTCGGAGTGCTAGGTGTAGTCCTCCATAAAGGAGTTGCCGCCCTGCAGACCGGTTGACATCACCTTCATGATGCGAAGAAGGGCGGCGGATTTCCCGACGCCTTc                                                                                         >  2:391962/1‑251 (MQ=255)

                                                                                                                                                        ccGGTTTGGGTATTGAAGATCAGTCGGGCAG‑GATCGTCCTTACCACCAATACCGAG‑ATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAAT‑CGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGACATCACCTTCATGATGCGAAGCAGGGCGGCGGTTTTCCAGTCGCCTTc                                                                                         <  1:200542/251‑1 (MQ=255)

                                                                                                                                                        ccGGTTTGGGTAGTGAAGATCAGGCGGGCAG‑GATCGTCCTTACCACCAATACCGAG‑ATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAAT‑CGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGATATCACCTTCAGGATGCGAAGCAGGGCGGCGGTTTTCCAGTCGCCTTc                                                                                         <  1:832022/251‑1 (MQ=255)

                                                                                                                                                          ggTTTGGGTATTGAAGATCAGTCGGGCAG‑GATCGTCCTTACCACCAATACCGAG‑ATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAAT‑CGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGCGGTGCCGCCCTGCAGACCGGTTGACATCACCTTCATGCTGCGAAGCAGGGCGGCGGTTTTCCAGGCGCCTTCGc                                                                                       >  2:939084/1‑251 (MQ=255)

                                                                                                                                                            tttGGGTATTGAAGATCAGTCGGGCAG‑GATCGTCCTTACCACCAATACCGAG‑ATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAAT‑CGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGACATCACCTTCATGATGCGAAGCAGGGCGGCGGTTTTCCAGTCGCCTTCGccc                                                                                     >  1:940751/1‑251 (MQ=255)

                                                                                                                                                                ggTATTGAAGAGCAGTCGGGCAG‑GAGCGTTCTTACCACCAATACCGAG‑ATGCTGAACGTCGAGGAGCGGTTTCTCTTCTACGGCAAT‑CGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCGGCAGACCGTGTGACATCACCTTCATGATGCGAAGCAGGGCGGCGGTTTTCCAGTCGCCTTCGCCCGCaa                                                                                 <  2:988079/251‑1 (MQ=255)

                                                                                                                                                                 gTATTGAAGATCAGTCGGGCAG‑GATCGTCCTTACCACCAATACCGAG‑ATGCTGAACGGCGAGGATCGGTTTCTCTTCTACGGCAAT‑CGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGACATCACCTTCATGATGCGAAGCAGggcggcggt                                                                                                        <  2:921659/227‑4 (MQ=255)

                                                                                                                                                                   aTTGAAGATCAGTCGGGCAG‑GATCGTCCTTACCACCAATACCGAG‑ATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAAT‑CGACGGGCAGACTTCCAGCATATGGGAGCCGAGCATCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTTACATCACCTTCATGATGCGAAGCAGGGCGGCGGTTTTCCAGTCGCCTTCGCCCGCAAAGc                                                                              <  2:837242/251‑1 (MQ=255)

                                                                                                                                                                      gAAGATCAGTCGGGCAG‑GATCGTCCTTACCACCAATACCGAG‑ATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAAT‑CGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGACATCACCTTCATGATGCGAAGCAGGGCGGCGGTTTTCCAGTCGCCTTCGCCCGCAAAGCCGt                                                                           >  1:971548/1‑251 (MQ=255)

                                                                                                                                                                               tcGGGCAG‑GATCGTCCTTACCACCAATACCGAG‑ATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAAT‑CGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGACATCACCTTCATGATGCGAAGCAGGGCGGCGGTTTTCCAGTCGCCTTCGCCCGCAAAGCCGTAACCctgct                                                                  >  1:249732/2‑251 (MQ=255)

                                                                                                                                                                                 gggCAG‑GATCGTCCTTACCACCAATACCGAG‑ATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAAT‑CGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGACATCACCTTCATGATGCGAAGCAGGGCGGCGGTTTTCCAGTCGCCTTCGCCCGCAAAGCCGTAACCCtgctgc                                                                <  1:893266/251‑1 (MQ=255)

                                                                                                                                                                                   gCAG‑GATCTTCCTTACCACCAATACCGAG‑ATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAAT‑CGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGACATCACCTTCATGATGCGAAGCAGGGCGGCGGTTTTCCAGTCGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCAt                                                              <  2:560790/251‑1 (MQ=255)

                                                                                                                                                                                         aTCGTCCTTACCACCAATACCGAG‑ATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAAT‑CGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAACGGAGGTGCCGCCCTGCAGACCGGTTGACATCACCTTCATGATGCGAAGCAGGGCGGCGGTTTTCCAGTCGCCTTCGCCCGCAAAACCGTAACCCTGCCGCATCAGa                                                          >  2:491378/1‑250 (MQ=255)

                                                                                                                                                                                            gTCCTTACCACCAATACCGAG‑ATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAAT‑CGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGACATCACCTTCATGATGCGAAGCAGGGCGGCGGTTTTCCAGTCGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCt                                                      >  1:544822/1‑251 (MQ=255)

                                                                                                                                                                                              acTTACCACCAATACCGAG‑ATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAAT‑CGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTACATAAAGGAGGTGCAGCACTGCAGACCGGTTGACATCACCTTCATGATGCGAAGCAGGGCGGCGGCTTTCCAGGCGCCTTCGCCCGCAAAGCCGCAACCCTTCCGCATCataccctct                                                    >  2:714060/2‑243 (MQ=255)

                                                                                                                                                                                                 taacaccaATACCGAG‑ATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAAT‑CGACGGGCAGTATTCCAGCATATGGGAGCCGAGAACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGACATCACCTTCATGATGCGAAGCAGGGCGGCGGTTTTCCAGTCGCCTTCGCCCGCAAAGCCGTAACCCGGCTGCATCAGACGCTGTa                                                   <  2:515350/246‑1 (MQ=255)

                                                                                                                                                                                                  accaccAATACCGAG‑ATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAAT‑CGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGACATCACCTTCATGATGCGAAGCAGGGCGGCGGTTTTCCAGTCGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACgg                                                >  1:347692/1‑251 (MQ=255)

                                                                                                                                                                                                  accaccAATACCGAG‑ATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAAT‑CGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGACATCACCTTCATGATGCGAAGCAGGGCGGCGGTTTTCCAGTCGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACgg                                                >  1:848015/1‑251 (MQ=255)

                                                                                                                                                                                                  accaccAACACCGAG‑ATGCGGAACGTCGAGGATCGGTTTCTCTTCTACGGCAAG‑CGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGACATCACCTTCATGATGCGAAGCAGGGCGGCGGTTTTCCAGTCGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACgg                                                <  2:645861/251‑1 (MQ=255)

                                                                                                                                                                                                   ccaccaATACCGAG‑ATGCTGAACGTCGAGGCTCGGTTTCTCTTCTACGGCAAT‑CGACGGGCCGACTTCCATCATATGGTTGCCGTGCACCATGTCATCACCTCTCTCGAAGTGATAGGTGTAGTACTCCATAAAGGAGGGGCCGCCCTGCCGACCGGTTGACATCACCGTCATGATGCGAAGCCGCGCGGCGGTTTTCCAGTCGCCTTCTCCCGCATAGCCTTAACCCTGCTGCATCAGACGCTgcactg                                                <  2:586180/250‑6 (MQ=255)

                                                                                                                                                                                                     accaATACCGAG‑ATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAAT‑CGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGACATCACCTTCATGATGCGAAGCAGGGCGGCGGGTTTCCAGTCGCCTTCGCCCGCAAAGCCGTAACCCTGCCGCATCAGCCGCTGTACGGccc                                             >  2:46091/1‑250 (MQ=255)

                                                                                                                                                                                                      ccaATACCGAG‑ATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAAT‑CGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGACATCACCTTCATGATGCGAAGCAGGGCGGCGGTTTTCCAGTCGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTCCGGcc                                              >  1:626106/1‑249 (MQ=255)

                                                                                                                                                                                                       caATACCGAG‑ATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAAT‑CGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGACATCACCTTCATGATGCGAAGCAGGGCGGCGGTTTTCCAGTCGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGa                                           <  2:258063/251‑1 (MQ=255)

                                                                                                                                                                                                         aTACCGAG‑ATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAAT‑CGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGACATCACCTTCATGATGCGAAGCAGGGCGGCGGTTTTCCAGTCGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGAcc                                         <  1:486716/251‑1 (MQ=255)

                                                                                                                                                                                                              gag‑aTGCTGACCGTCGAGGATCGGTTTCTCTTCTACGGCAAT‑CGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGACATCACCTTCATGCTGCGAAGCAGGACGGCGGTTTTCCAGTCGCCTACGCCCGCAAAGCCGTAAACCTGCTGCATCAGACGCTGGACGGCCAGACCTGGa                                     >  1:952201/1‑250 (MQ=255)

                                                                                                                                                                                                              gag‑aTGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAAT‑CGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGACATCACCTTCATGATGCGAAGCAGGGCGGCGGTTTTCCAGTCGCCTTCGccc                                                                                     <  1:533096/202‑1 (MQ=255)

                                                                                                                                                                                                               ag‑aTGCTGAACGTCGCGGATCGGTTTCTCTTCTACGGCAAT‑CGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGACATCACCTTCATGATGCGAAACAGGGCGGCGGTTTTCCAGTCGCCTTCCCCCGCACAGCCGTAACCCTGCTGCATCAGACGCTGTCCGGCCAGACCTGGaac                                   >  1:183667/1‑250 (MQ=255)

                                                                                                                                                                                                                   tgctgcACGTCGAGGATCGGTTTCTCTTCTACGGCAAT‑CGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGGGATAGGTGTAGTCCTCCCTAACGGAGGTGCCGCCCTGCAGACCGGTTGACATCATCTTCATGATGCGAGGCAGGACGGCGGGTTTCCAGGCGCCCTTTCCCGCACAGCCACAACCCGACTGCTTCAGACCCTGTACGGCCatgccgggcggatg                                >  2:197150/1‑238 (MQ=255)

                                                                                                                                                                                                                      ggAACGTCGAGGATCGGTTTCTCTTCTACGGCAAT‑CGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGACATCACCTTCATGATGCGAAGCAGGGCGGCGGTTTTCCAGTCGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGACCTGGAAGCTGttt                             <  2:255667/250‑1 (MQ=255)

                                                                                                                                                                                                                       gaactgcgtggaactgTTTCCCTTCTGCGGCCAG‑CGACGGGCAGCGTTCCAACACATGGTAGCTGAGCACCAGGTCATGTCTTTTCTCGATGTTATAGGGTTAGTCCGCCATAAAGGAGGTGCCGCCCTGCAGACCGTTTGTCATCACCGTCCTGATGCGAAGCAGGGCGGCGGTTTTCCAGTCGCCTTCGCCCGCAAAGCCGTATCCCTGCTTCAGCAGACTCTGTACGGCCAGTCCTGGAAGCTGttt                             <  2:467742/235‑1 (MQ=255)

                                                                                                                                                                                                                       gAACGTCGAGGATCGGTTTCTCTTCTACGGCAAT‑CGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGACATCACCTTCATGATGCGAAGCAGGGCGGCGGTTTTCCAGTCGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGACCTGGAAGCTGTTTc                            <  1:442615/251‑1 (MQ=255)

                                                                                                                                                                                                                       gAACGTCGAGGATCGGTTTCTCGTCTACGGCAAG‑CGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGACATCACCTTCATGATGCGAAGCAGGGCGGCGGTTTTCCAGTCGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGACCTGGAAGCTGtt                              <  1:934292/249‑1 (MQ=255)

                                                                                                                                                                                                                       gAACGGCGAGGATCGGTTTCTCTTCTACGGCAAT‑CGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGACATCACCTTCATGATGCGAAGCAGGGCGGCGGTTTTCCAGTCGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGACCTGGAAGCTGtt                              <  1:601392/249‑1 (MQ=255)

                                                                                                                                                                                                                        aaCGGCGAGGATCGGGTTCGTTTCTACGGCAAT‑CGACGGGCAGACTTCCAGCATATGGGTGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGACATCACCTTCATGATGCGAAGCAGGGCGGCGGTTTTCCAGTCGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGACCTGGAAGCTGTTTCa                           <  2:151791/251‑1 (MQ=255)

                                                                                                                                                                                                                           gTCGAGGATCGGTTTCTCTTCTGCGGCGAT‑CGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGgt                                                                                                                                                                                   <  2:423340/96‑1 (MQ=255)

                                                                                                                                                                                                                           gTCGAGGATCGGTTTCTCTTCTACGGCAAT‑CGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGACATCACCTTCATGATGCGAAGCAGGGCGGCGGTTTTCCAGTCGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGACCTGGAAGCTGTTTCAGAc                        <  1:413107/251‑1 (MQ=255)

                                                                                                                                                                                                                             cGAGGATCGGTTTCTCTTCTACGGCAAT‑CGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGACGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGACATCACCTTCATGATGCGAAGCAGGGCGGCGGTTTTCCAGTCGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCCGACGCTGTACGGCCAGACCTGGAAGCTGTTTCAGACCg                      >  2:112799/1‑251 (MQ=255)

                                                                                                                                                                                                                                 gATCGGTTTCTCTTCTACGGCAAT‑CGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGACATCACCTTCATGATGCGAAGCAGGGCGGCGGTTTTCCAGTCGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGACCTGGAAGCTGTTTCAGACCg                      >  1:99529/1‑247 (MQ=255)

                                                                                                                                                                                                                                             ttctACGGCAAT‑CGCCGGGCAGACTTCCAGCATAGGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGATGACATCACCTTCATGATGCGAAGCAGGGCGGCGGTTTTCCAGGCGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGCCCTGGCAGCTGTTTCCGCCCATACAAATCTTCaacgg      >  2:147973/1‑248 (MQ=255)

CGGAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATGCAGTTAACCAGTAGACGGTAACGATCGCCGAGATCAATCAAGCTGGCGACAATCGCTGGGCCGGTTTGGGTATTGAAGATCAGGCGGGCAG‑GATCGTCCTTACCACCAATACCGAG‑ATGCTGAACGTCGAGGATCGGTTTCTCTTCTGCGGCGAT‑CGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGACATCACCTTCATGATGCGAAGCAGGGCGGCAGTTTTCCAGTCGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGACCAGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGT  >  NC_000913/67020‑67509

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`