BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	67,171	G→A	G389G (GGC→GGT)	araA ←	L‑arabinose isomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	67,171	0	G	A	98.0%	465.0 / NA	154	G389G (GGC→GGT)	araA	L‑arabinose isomerase
Reads supporting (aligned to +/- strand): ref base G (2/1); major base A (76/72); minor base C (3/0); total (81/73)
Fisher's exact test for biased strand distribution p-value = 2.47e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 154 total aligned reads displayed.

AATGTCGTGCATCTCGGCGAATTGGCGCATATCGTTGAGGTTCAGTGCATGGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATGCAGTTAACCAGTAGACGGTAACGATCGCCGAGATCAATCAAGCTGGCGACAATCGCTGGGCCGGTTTGGGTATTGAAGATCAGGCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTGCGGCGAT‑CGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGACATCACCTTCATGATGCGAAGCAGGGCGGCAGTTTTCCAGT  >  NC_000913/66925‑67415

aaTGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCgg                                                                                                                                                                                                                                                   >  1:955330/1‑251 (MQ=255)

 aTGTCGTGCATCTCGGCAAACTGGCGAATATCGTTGAGGTTAAGCGCATGGCTGAAGACGGTATGGAGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATCCAGTTAACCAGCAGACGGTACAGATCGCCGAGATCACTCAGGCTGGCGCCACTCGCTGGACCggg                                                                                                                                                                                                                                                  >  2:630943/1‑250 (MQ=255)

  ggTCGTGCATCTCGGCGAACTGGTGCATATCGTTGAGGTTCAGCGCATGGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGtt                                                                                                                                                                                                                                                 <  1:652843/250‑1 (MQ=255)

   gaggtgccttccGGCGAATTGGCGCATATCGTTGAGGTTCAGCGCATGGCTGAAGACGTTATGGTGCTCGTCGCCAGCGAGGATCCTCGCTTCGGAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCTCCGGCAGTTTCGGCAGGTAGTGCGGTGCTTTCACCTTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAAGCAGGCTGGCGACAATCGCTGGACCgggtt                                                                                                                                                                                                                                                <  2:82073/240‑4 (MQ=255)

     cGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCGTGGCTGAAGACGGTATGGTGAGCGCCACCAGCGAGGATACACGCTTAGGAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGCTACAGGTAACCAGCAGACGGTAACGATCGCCGCGATAAATCAGGATGGCGACAATCGCTGGACCGGTTTgg                                                                                                                                                                                                                                              >  1:586180/1‑251 (MQ=255)

      gggCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGAATGTCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTggg                                                                                                                                                                                                                                             <  2:612003/249‑1 (MQ=255)

      gTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTggg                                                                                                                                                                                                                                             >  1:931356/1‑251 (MQ=255)

       ggCATCGCGGCGAAGTGGCGCATATCGTTGATGGTCAGCGCATGGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGAGCCTCGCTTCGGAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTGCGGCAGGGAGGGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGAGCGCCGAGAGCAATCAGGCTGGCGACAATCGCTGGATCGGTGTGGGt                                                                                                                                                                                                                                            <  2:168354/250‑1 (MQ=255)

            ctcGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGCTGAAGACGGTATGGCGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCCCCGGCAGTTTCGGCAGGGAGTGCGGGGCTTTCACCGTGTCGCTCCCGTTAACCAGCAGCCGGTTCCGAGCGCCGTGCTCCATCCGGCTGGCGACACTCGCTGGCCCGGtttccgagttgg                                                                                                                                                                                                                                       >  2:185765/1‑242 (MQ=255)

              cGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGATCCACGCGTCGGAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCATGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAg                                                                                                                                                                                                                                     <  1:36119/251‑1 (MQ=255)

              cGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGCTGAAGACGGTATGGGGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGaa                                                                                                                                                                                                                                      <  2:369577/250‑1 (MQ=255)

                    actGGCGCATATCGTTGAGGGTCAGCGGATGGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGATCCACGCATCGGAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTGCGGCAGGGAGTGCGTTGTTTTCACCGTGTCGATACAGTTACCCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAgt                                                                                                                                                                                                                               <  1:892071/249‑2 (MQ=255)

                         cgcATATCGTTGAGGTTCAGCGCATGGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGc                                                                                                                                                                                                                          <  1:595543/251‑1 (MQ=255)

                           cATATCGTTGAGGTTCAGCGCATGGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGGGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGCCAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGc                                                                                                                                                                                                                          >  1:151791/1‑249 (MQ=255)

                            atatCGTTGAGGTTCAGCGCATGGCTGAAGACGGTATGGTGCGCGCCCCCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCACATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAgg                                                                                                                                                                                                                       <  2:217463/251‑1 (MQ=255)

                             tatCGTTGAGGTTCAGCGCATGGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGGGGGCGGTGGTTTCACCGGGTCGATACCGTTAACCAGCAGACGGTAACGATCGCCGGGGTCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGGATTGAAGATccatcgggcaggg                                                                                                                                                                                                                      >  2:695448/1‑239 (MQ=255)

                                     aGGTTCAGCGCATGGCTGAAGAGGGTATGGTGCGCGCCACCATCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATCCAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTc                                                                                                                                                                                                                 <  1:211415/248‑1 (MQ=255)

                                           cgcgCATGGCTGAAGCCGGTATGGTGCGCGCCTCCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACTGGGTCGATACAGTTATCCAGCAGACGGTAACTATCGCCGAGATCAATCAGGCTGGCGACAATCTCTGGACCTGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTaccacc                                                                                                                                                                                                        <  1:877953/248‑1 (MQ=255)

                                           agcgCATGGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTaccacc                                                                                                                                                                                                        >  1:397858/4‑251 (MQ=255)

                                                    cTGAAGACGGTATGGTGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCg                                                                                                                                                                                                 >  1:625742/1‑249 (MQ=255)

                                                     tGAAGACGGTATGGTGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGGCCTTACCAACCATACCgcg                                                                                                                                                                                               >  1:660992/1‑248 (MQ=255)

                                                     tGAAGACGGTATGGAACGCGCAACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCAGCAGGGGGTGCGGAGTTTTCCCCGTGTCGATACAGTTAACCCGCAGCCGGTAAAGATCGCCGAGATTAATCCGGCTGGCGACAATCGCTGGACCGGTTTGGGAACTGAAGATCCAGCGGACAAGAACGTCCTTCACACCaagactg                                                                                                                                                                                                 >  2:929622/1‑243 (MQ=255)

                                                       aaGACGGTATGGTGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGCTCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGGTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATg                                                                                                                                                                                            <  1:475929/251‑1 (MQ=255)

                                                             gTATGGTGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAAc                                                                                                                                                                                      <  1:141894/251‑1 (MQ=255)

                                                              aaTGGTGCGCGCCACCAGCGAGGATCCACGCTTCGGAATCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGGTTTCACCGTGTCGATACAGTTAAACCGCAGACAGTAACGAACGCCGACATCAAACAGGCTTGCGACAATCGCTGGACCGGTTTTGGAATTGCAATTCAAGCGGGCAGGATCGTCCTTACCACCAATAcccagcagctgtacg                                                                                                                                                                                     >  2:250635/2‑238 (MQ=255)

                                                               aTGGTGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCCGGGGGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGAACGCCGAGATCAATCAGGCTGGCGCCAATCGCTGGCCAGGTTTGGGTATTGAAGATCAATCGGGCAGGATAGTCCTTACCACCaaacacgaggttctgaacgt                                                                                                                                                                                    >  2:835668/1‑233 (MQ=255)

                                                                 ggTGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGAGCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCg                                                                                                                                                                                  <  2:306699/251‑1 (MQ=255)

                                                                  gggcgcgcCCCCAGCGGGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCGCCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGGCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGCCCTTACCACCAATACCGAGATGCTGAACGTCGa                                                                                                                                                                                 <  2:158268/249‑1 (MQ=255)

                                                                  gTGCGCGCCCCCAGGTAGGATCCACGCTTAGGAAGCAGTTGGCAGCTGCGGTTGCGCTGTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTGTTCACCGTGACGATACAGTGAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCGGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTTCTTATCACCAATTCCGAGATGCTGAACGTCGa                                                                                                                                                                                 <  2:1233/251‑1 (MQ=255)

                                                                      gcgcCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGAt                                                                                                                                                                             <  1:734619/251‑1 (MQ=255)

                                                                       cgcCACCAGCGAGGATCCACGCTTCGGCAGCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGGGTTTTCACCGTGTCGATCCAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGGGACAATCGGTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGGTGCTGAACGTAGAGGagg                                                                                                                                                                            >  2:629833/1‑249 (MQ=255)

                                                                                 gAGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCtctt                                                                                                                                                                  >  1:265357/1‑251 (MQ=255)

                                                                                      cccACGCTTCGGAAGCAGTTTGCAGTGCCGGTTGCGCTTTCCACAGCGCATGCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTTGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCtcttctacg                                                                                                                                                             <  2:332450/250‑4 (MQ=255)

                                                                                         aCGCTTCGGAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTacggca                                                                                                                                                          <  1:401323/251‑2 (MQ=255)

                                                                                          cGCTTCGGAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGTTCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTacggca                                                                                                                                                          >  1:134475/1‑249 (MQ=255)

                                                                                          cGCTTCGGAAGAAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAACAGACGGTAACGATCGCCGCGAACAATCAGGCTGGCGACAATCGCTGGACCGGGTTGGGCATTGCAGATCAGGCGGGCAGGATCGTCCTTACCCCCAATACCGGGAGGGCGAAGGCCGAGGCTAGGTTTCTCTTCTacggca                                                                                                                                                          >  2:502690/1‑249 (MQ=255)

                                                                                           acTTCGGAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAAAGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGGCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGACGAGGATCGGTTTCTCTTCTacggcca                                                                                                                                                         >  2:767148/2‑248 (MQ=255)

                                                                                               cGGAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAAT‑CGAc                                                                                                                                                   >  1:987401/1‑251 (MQ=255)

                                                                                               cGGAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAAT‑CGAc                                                                                                                                                   <  1:5616/251‑1 (MQ=255)

                                                                                                ggAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCTCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAAT‑CGACg                                                                                                                                                  <  1:62/251‑1 (MQ=255)

                                                                                                 gAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGTGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAAT‑CGACgg                                                                                                                                                 <  1:100231/251‑1 (MQ=255)

                                                                                                  aaGCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGGGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTCCCACCACTACCGAGCTGCTGAACGTCGGGGATCGGGTTCTCTTTTACGGCAAT‑CGACggg                                                                                                                                                >  2:273251/1‑251 (MQ=255)

                                                                                                  aaGCAGATGGCAGATCCGGTTGAGCATTACACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACCGTTAACCAGCAGACGGACACGATAGCCGAGATCCATCAGGCTGGCGCCAATCGCCGGACAGGTTTGGGGATTGAAGATCCGAGGGGCAGGATGGACCTTACCACCAATACCGAGATGCTGAACGTCGGGGAGAGGTTTCtctttgacgaccaacgacggg                                                                                                                                                 >  2:937441/1‑234 (MQ=255)

                                                                                                    gCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAAT‑CGACGGGCa                                                                                                                                              <  1:934583/251‑1 (MQ=255)

                                                                                                          ggCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAAT‑CGACGGGCAGACTTc                                                                                                                                        >  1:291182/1‑251 (MQ=255)

                                                                                                            cAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCCGTTTCGGCAGGGTGTGCGGTGTTTTAACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGCGATCAATCAGGCTGGCGACAATCCCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAAT‑CGACCGGCCGGCTTccc                                                                                                                                      >  2:442615/1‑250 (MQ=255)

                                                                                                                 ccGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAAT‑CGACGGGCAGACTTCCAGCata                                                                                                                                 <  1:245260/251‑1 (MQ=255)

                                                                                                                  cGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAAT‑CGACGGGCAGACTTCCAGCatat                                                                                                                                <  1:167525/251‑1 (MQ=255)

                                                                                                                   ggTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCCCCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCCATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGG‑AATACGACGGGCCGACTTCCCGCATATg                                                                                                                               >  2:434601/1‑251 (MQ=255)

                                                                                                                   ggTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAAT‑CGACGGGCAGACTTCCAGCATATg                                                                                                                               >  1:255667/1‑251 (MQ=255)

                                                                                                                     ttGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAAT‑CGACGGGCAGACTTCCAGCATATggg                                                                                                                             >  1:103894/1‑251 (MQ=255)

                                                                                                                        cgcTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAAT‑CGACGGGCAGACTTCCAGCATATGGGAGc                                                                                                                          <  1:804269/251‑1 (MQ=255)

                                                                                                                                    cgcATCCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCCCCGTGTCGATACAGTTAACCAGCAGACGGGAACGAGCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATGCCGAGATGCTTAACGTCGAGGATCGGTTTTTCTTCTACGGCAAG‑CGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGt                                                                                                              <  2:429794/251‑1 (MQ=255)

                                                                                                                                      cATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGCGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAAT‑CGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTc                                                                                                             >  1:523458/1‑250 (MQ=255)

                                                                                                                                        ttCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAAT‑CGACGGGCAGCCTTCCAGCATATGGGAGCCGAGCACCAGGTCAtt                                                                                                          >  1:181715/1‑251 (MQ=255)

                                                                                                                                          cGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAAT‑CGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTa                                                                                                         <  2:475848/250‑1 (MQ=255)

                                                                                                                                            ccACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAAT‑CGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCt                                                                                                      <  1:274558/251‑1 (MQ=255)

                                                                                                                                              aCCGGCAGTTTCGGCAGGGAGGTCGGTGTTTTCACCGTTTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGCGATCAATCAGGCTGGCGACAATCGCTGGACCGGGTTGGGTATTGAAGATCAGTCGGGCAGGCTCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGATTCTCTTCTACGGCAAT‑CGCCGGGCAGAATTCCAGCATATGGGGGCCGAGCACCCGGTCATTACCttt                                                                                                    >  2:413107/1‑251 (MQ=255)

                                                                                                                                                 ggCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCCGTCGGGCCGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAAT‑CGACGGGCAGACTTCCCGCATATGGGGGCCGAGCACCGGGTCCTTACCTTtctc                                                                                                 >  2:876194/1‑251 (MQ=255)

                                                                                                                                                   cAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAAT‑CGACGGGCAGACTTCCAGCATATGGGAGCCGCGCACCAGGTCATTACCTTTCTCg                                                                                                >  1:703611/1‑250 (MQ=255)

                                                                                                                                                    aGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCAGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCCCCAATACCGAGATGCTGAACGACGAGGATCGGTTTCTCTTCTACGGCAAT‑CGACGGGCAGACTTCCCGCATATGGGAGCCCAGCACCAGGGCATTCCCTTTCTCgc                                                                                               >  2:90047/1‑249 (MQ=255)

                                                                                                                                                     gTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAAT‑CGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGa                                                                                               >  1:486413/1‑249 (MQ=255)

                                                                                                                                                        tCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAAT‑CGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTg                                                                                           <  1:853322/250‑1 (MQ=255)

                                                                                                                                                             aGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAAT‑CGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGgt                                                                                     <  2:461821/251‑1 (MQ=255)

                                                                                                                                                             aGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAAGCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCGTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTGCTACGGCAAT‑CGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGgt                                                                                     <  1:47169/251‑1 (MQ=255)

                                                                                                                                                                   tGCGGTGTTTTCACCGTGTCGATACAGTTAACAAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGAACGGTTTCTCTTCTACGGCAAT‑CGACGGGCAGACTCCCAGCATATGGGAGCCGACCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGtc                                                                               >  2:963392/1‑251 (MQ=255)

                                                                                                                                                                     cGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGAAGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGGATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGGTCGGTTTCTCTTCTACGGCAAT‑CGACGGGCAGAATTACAGCATCTGGGAGCCGAGCACCAGGTCATTACCTTTCGCGACGTGATAGGTGTAGacct                                                                             >  2:566812/1‑251 (MQ=255)

                                                                                                                                                                         gtTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAAT‑CGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGACATTACCTTTCTCGAAGGGATAGGTGTAGTCCTCCa                                                                          >  1:707645/1‑250 (MQ=255)

                                                                                                                                                                                ccGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAAT‑CGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAAGTCATTACCTTTCTCGAAGGGATAGGTGTAGTCCTCCATAAagg                                                                    >  2:837171/1‑249 (MQ=255)

                                                                                                                                                                                ccGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAAGCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAAT‑CGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAaggag                                                                  <  1:325819/251‑1 (MQ=255)

                                                                                                                                                                                 cGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAAT‑CGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAATCCTCCCTAAaggagg                                                                 >  2:773408/1‑251 (MQ=255)

                                                                                                                                                                                   tgtCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAAT‑CGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGCGGTg                                                               >  1:97405/1‑251 (MQ=255)

                                                                                                                                                                                    gtCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAAT‑CGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGCGTAGGCCTCCATAAAGGAGGTgc                                                              >  1:123366/1‑251 (MQ=255)

                                                                                                                                                                                     tCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAAT‑CGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGGGATAGGTGTAGTCCTCCATAAAGGAGGTgcc                                                             >  2:878783/1‑251 (MQ=255)

                                                                                                                                                                                              ttAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAAT‑CGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGc                                                      >  1:178277/1‑249 (MQ=255)

                                                                                                                                                                                               tAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAAT‑CGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGTTGCCGCCCTGCAg                                                    <  1:758493/250‑1 (MQ=255)

                                                                                                                                                                                                 aCCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAAT‑CGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGAcc                                                 <  1:410002/251‑1 (MQ=255)

                                                                                                                                                                                                  ccAGCAGATGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAAT‑CGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCg                                                <  1:512931/251‑1 (MQ=255)

                                                                                                                                                                                                   cagcaGACGGTAACGATCGCCGAGAGCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAAT‑CGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCgg                                               <  2:599797/247‑1 (MQ=255)

                                                                                                                                                                                                   caacatgagggaacagtcgcatagaTCAATCAGGCTGGCGCAAATCGCTGGATCGGTTTGGGTATTGAAGATCAGTCTGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAAT‑CGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGAGAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCgg                                               <  2:300319/229‑1 (MQ=255)

                                                                                                                                                                                                    agcaGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAAT‑CGCCGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGt                                              >  1:661679/4‑251 (MQ=255)

                                                                                                                                                                                                     gcaGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAAT‑CGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGtt                                             >  1:165893/3‑251 (MQ=255)

                                                                                                                                                                                                      caGACGGTAACGATCGCCGAGATCAATCAGTCTGGCGACAAGCGCTGGACCGGTTTGCGTATTGACGATCAGTCGGGCAGGATCGTCCTTACCACCTATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAAT‑CGACGGGCAGCCTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTg                                            <  1:535077/250‑1 (MQ=255)

                                                                                                                                                                                                        gACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAAT‑CGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGAc                                          <  1:310691/251‑1 (MQ=255)

                                                                                                                                                                                                           ggTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAAT‑CGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGACATc                                       >  1:281574/1‑251 (MQ=255)

                                                                                                                                                                                                            gTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAAT‑CGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGACATCa                                      <  1:695448/251‑1 (MQ=255)

                                                                                                                                                                                                              aaCGATCGCCGAGAGCAATCAGGCGGGCGACAAATGCTGGACAGGTTTGGGTAATGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGGCGAGGATCGGGTTCTCTTCTACGGCAAT‑CGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGAAATCAc                                     <  2:349276/250‑1 (MQ=255)

                                                                                                                                                                                                               aCGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAAT‑CGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGACATCACCt                                   <  2:172407/251‑1 (MQ=255)

                                                                                                                                                                                                                 gATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAAT‑CGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGACATCACCTTc                                 >  1:560790/1‑251 (MQ=255)

                                                                                                                                                                                                                     gCCGAGATCAATCAAGCTGGCGACAATCGCTGGGCCGGTTTGGGTATTGAAGATCAGGCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTGCGGCGAT‑CGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGGTAGGTGTAGTCCTCACTAAAGGAGGGGCCGCCCTGCAGACCGGTTGACATCACCTTCatg                              >  2:493901/1‑250 (MQ=255)

                                                                                                                                                                                                                       cGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAAT‑CGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGACATCACCTTCatgatg                           >  1:327982/1‑251 (MQ=255)

                                                                                                                                                                                                                          gaTCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAAT‑CGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGACATCACCTTCATGATGCGa                        <  1:902378/251‑1 (MQ=255)

                                                                                                                                                                                                                             caatcagGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAAT‑CGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGGGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGACATCACCTTCATGATGCGAAGc                     >  1:342528/1‑251 (MQ=255)

                                                                                                                                                                                                                              aagcagggtGGCGAAAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGGCGAGGATCGGTTTCTCTTCTACGGCAAT‑CGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCGTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGGGCCGCCCTGCAGACCGGTTGACATCACCTTCATGATGCGAAGCa                    <  2:938169/243‑1 (MQ=255)

                                                                                                                                                                                                                                 caggCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAAT‑CGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGACATCACCTTCATGATGCGAAGCAggg                 <  2:702692/248‑1 (MQ=255)

                                                                                                                                                                                                                                 caggCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGACCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAAT‑CGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGACATCACCTTCATGATGCGAAGCAggg                 <  2:977750/248‑1 (MQ=255)

                                                                                                                                                                                                                                      gggCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGAGCGGTTTCTCTTCTACGGCAAT‑CGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGACATCACCTTCATGATGCGAAGCAGggcggcg            <  2:955330/250‑2 (MQ=255)

                                                                                                                                                                                                                                       ggCGACAATCGCTGGACCTGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAAT‑CGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGACATCACCTTCATGATGCGAAGCAGggcggc             <  1:213804/249‑1 (MQ=255)

                                                                                                                                                                                                                                           aCAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAAT‑CGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGACATCACCTTCATGATGCGAAGCAGGGCGGCGGtttt       <  1:856076/251‑1 (MQ=255)

                                                                                                                                                                                                                                                cGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAAT‑CGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGACATCACCTTCCTGATGCGAAGACGGGCGGCGGTTTTcccgt  >  2:607793/1‑248 (MQ=255)

AATGTCGTGCATCTCGGCGAATTGGCGCATATCGTTGAGGTTCAGTGCATGGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATGCAGTTAACCAGTAGACGGTAACGATCGCCGAGATCAATCAAGCTGGCGACAATCGCTGGGCCGGTTTGGGTATTGAAGATCAGGCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTGCGGCGAT‑CGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGACATCACCTTCATGATGCGAAGCAGGGCGGCAGTTTTCCAGT  >  NC_000913/66925‑67415

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`