BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	67,152	A→G	L396L (TTG→CTG)	araA ←	L‑arabinose isomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	67,152	0	A	G	98.0%	488.9 / ‑0.7	150	L396L (TTG→CTG)	araA	L‑arabinose isomerase
Reads supporting (aligned to +/- strand): ref base A (2/1); new base G (74/72); total (76/74)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.11e-01

Only 100 of 152 total aligned reads displayed.

TATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAATTGGCGCATATCGTTGAGGTTCAGTGCATGGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATGCAGTTAACCAGTAGACGGTAACGATCGCCGAGATCAATCAAGCTGGCGACAATCGCTGGGCCGGTTTGGGTATTGAAGATCAGGCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTGCGGCGAT‑CGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGACATCACCTTCATGATGCGAAGC  >  NC_000913/66906‑67396

tATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCt                                                                                                                                                                                                                                                    <  1:365829/250‑1 (MQ=255)

   caatcaCCGTGATTTCAATGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCg                                                                                                                                                                                                                                                >  1:977750/1‑251 (MQ=255)

   caataaCCGTGATGTCAATGTCGTGCCTCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCg                                                                                                                                                                                                                                                <  2:271955/251‑1 (MQ=255)

    aatcaCCGTGATT‑CAATGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGCTGAAGACGGTATGGGGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGGGTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGGTAACCAGCAGAAGGTAACGATCGCCGAGAGCAATCAGGCTGGCGAc                                                                                                                                                                                                                                              >  1:655792/1‑251 (MQ=255)

       caCCGTGATTTCAATGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACa                                                                                                                                                                                                                                             <  2:120580/250‑1 (MQ=255)

                 tCAATGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCCGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTggacc                                                                                                                                                                                                                                  >  1:525423/1‑248 (MQ=255)

                 tCAATGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTggacc                                                                                                                                                                                                                                  >  1:709733/1‑248 (MQ=255)

                 tCAATGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTggac                                                                                                                                                                                                                                   <  1:575496/250‑3 (MQ=255)

                  cAATGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTggacc                                                                                                                                                                                                                                  <  1:145590/250‑4 (MQ=255)

                  cAATGGCGTGCATCGCGGCGAACGGGCGCATATCGTTGAGGTTCAGCGCATGGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTggacc                                                                                                                                                                                                                                  <  2:342012/250‑4 (MQ=255)

                   aaTGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCgg                                                                                                                                                                                                                                >  1:955330/1‑251 (MQ=255)

                   aaTGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGGTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGGTACAGTTAACCAGCAGACGGGAACGGTCGCCGAGATCAATCAGGCTGGCGACAAACCCTGGACCgg                                                                                                                                                                                                                                >  2:310691/1‑251 (MQ=255)

                    aTGTCGTGCATCTCGGCAAACTGGCGAATATCGTTGAGGTTAAGCGCATGGCTGAAGACGGTATGGAGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATCCAGTTAACCAGCAGACGGTACAGATCGCCGAGATCACTCAGGCTGGCGCCACTCGCTGGACCggg                                                                                                                                                                                                                               >  2:630943/1‑250 (MQ=255)

                     ggTCGTGCATCTCGGCGAACTGGTGCATATCGTTGAGGTTCAGCGCATGGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGtt                                                                                                                                                                                                                              <  1:652843/250‑1 (MQ=255)

                      ggcGTGCATCGCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGtt                                                                                                                                                                                                                              <  2:709733/248‑1 (MQ=255)

                      gaggtgccttccGGCGAATTGGCGCATATCGTTGAGGTTCAGCGCATGGCTGAAGACGTTATGGTGCTCGTCGCCAGCGAGGATCCTCGCTTCGGAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCTCCGGCAGTTTCGGCAGGTAGTGCGGTGCTTTCACCTTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAAGCAGGCTGGCGACAATCGCTGGACCgggtt                                                                                                                                                                                                                             <  2:82073/240‑4 (MQ=255)

                        cGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCGTGGCTGAAGACGGTATGGTGAGCGCCACCAGCGAGGATACACGCTTAGGAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGCTACAGGTAACCAGCAGACGGTAACGATCGCCGCGATAAATCAGGATGGCGACAATCGCTGGACCGGTTTgg                                                                                                                                                                                                                           >  1:586180/1‑251 (MQ=255)

                         gggCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGAATGTCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTggg                                                                                                                                                                                                                          <  2:612003/249‑1 (MQ=255)

                         gTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTggg                                                                                                                                                                                                                          >  1:931356/1‑251 (MQ=255)

                          ggCATCGCGGCGAAGTGGCGCATATCGTTGATGGTCAGCGCATGGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGAGCCTCGCTTCGGAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTGCGGCAGGGAGGGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGAGCGCCGAGAGCAATCAGGCTGGCGACAATCGCTGGATCGGTGTGGGt                                                                                                                                                                                                                         <  2:168354/250‑1 (MQ=255)

                               ctcGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGCTGAAGACGGTATGGCGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCCCCGGCAGTTTCGGCAGGGAGTGCGGGGCTTTCACCGTGTCGCTCCCGTTAACCAGCAGCCGGTTCCGAGCGCCGTGCTCCATCCGGCTGGCGACACTCGCTGGCCCGGtttccgagttgg                                                                                                                                                                                                                    >  2:185765/1‑242 (MQ=255)

                                 cGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGCTGAAGACGGTATGGGGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGaa                                                                                                                                                                                                                   <  2:369577/250‑1 (MQ=255)

                                       actGGCGCATATCGTTGAGGGTCAGCGGATGGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGATCCACGCATCGGAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTGCGGCAGGGAGTGCGTTGTTTTCACCGTGTCGATACAGTTACCCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAgt                                                                                                                                                                                                            <  1:892071/249‑2 (MQ=255)

                                        ctGGCGCATATCGTTGAGGTTCAGCGCATGGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAgtc                                                                                                                                                                                                           >  1:186121/2‑249 (MQ=255)

                                         tGGCACATATCGTTGAGGTTCAGCGCATGGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGGGCGGTGTTTTCACCGTGGCGATACAGTTAACCAGCAGACGGTGACGATCGCCGAGAACAATCCGGCTTGCGACAATACCCGGACCGGATTGGGAATTGAAAATCAgacg                                                                                                                                                                                                          >  2:893266/1‑248 (MQ=255)

                                               atatCGTTGAGGTTCAGCGCATGGCTGAAGACGGTATGGTGCGCGCCCCCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCACATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAgg                                                                                                                                                                                                    <  2:217463/251‑1 (MQ=255)

                                                tatCGTTGAGGTTCAGCGCATGGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGGGGGCGGTGGTTTCACCGGGTCGATACCGTTAACCAGCAGACGGTAACGATCGCCGGGGTCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGGATTGAAGATccatcgggcaggg                                                                                                                                                                                                   >  2:695448/1‑239 (MQ=255)

                                                        aGGTTCAGCGCATGGCTGAAGAGGGTATGGTGCGCGCCACCATCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATCCAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTc                                                                                                                                                                                              <  1:211415/248‑1 (MQ=255)

                                                              cgcgCATGGCTGAAGCCGGTATGGTGCGCGCCTCCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACTGGGTCGATACAGTTATCCAGCAGACGGTAACTATCGCCGAGATCAATCAGGCTGGCGACAATCTCTGGACCTGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTaccacc                                                                                                                                                                                     <  1:877953/248‑1 (MQ=255)

                                                                        tGAAGACGGTATGGTGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGGCCTTACCAACCATACCgcg                                                                                                                                                                            >  1:660992/1‑248 (MQ=255)

                                                                          aaGACGGTATGGTGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGCTCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGGTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATg                                                                                                                                                                         <  1:475929/251‑1 (MQ=255)

                                                                                gTATGGTGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAAc                                                                                                                                                                   <  1:930553/251‑1 (MQ=255)

                                                                                gTATGGTGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAAc                                                                                                                                                                   <  1:141894/251‑1 (MQ=255)

                                                                                  aTGGTGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCCGGGGGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGAACGCCGAGATCAATCAGGCTGGCGCCAATCGCTGGCCAGGTTTGGGTATTGAAGATCAATCGGGCAGGATAGTCCTTACCACCaaacacgaggttctgaacgt                                                                                                                                                                 >  2:835668/1‑233 (MQ=255)

                                                                                    ggTGCGCTGCACCATCGAGGAGCCCCGCTTCGGAAGCAGTTGGCAGATCCGGTTGCGCTTCCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGGTCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGAACATTCGGGCAGGATCGTCCTTACCGCCAATACCGAGATGCTGAACGTc                                                                                                                                                                <  2:844684/250‑1 (MQ=255)

                                                                                    ggTGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGAGCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCg                                                                                                                                                               <  2:306699/251‑1 (MQ=255)

                                                                                     gggcgcgcCCCCAGCGGGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCGCCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGGCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGCCCTTACCACCAATACCGAGATGCTGAACGTCGa                                                                                                                                                              <  2:158268/249‑1 (MQ=255)

                                                                                     gTGCGCGCCCCCAGGTAGGATCCACGCTTAGGAAGCAGTTGGCAGCTGCGGTTGCGCTGTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTGTTCACCGTGACGATACAGTGAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCGGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTTCTTATCACCAATTCCGAGATGCTGAACGTCGa                                                                                                                                                              <  2:1233/251‑1 (MQ=255)

                                                                                      tGCGCGCCACCAGCGAGGATCCACGCTTAGGAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCCGACGGTAACGATCGACGCGATCACTCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGACCCATCGGGCCGGATCGTCCTTACCAACAATACCcagagcctggacatcg                                                                                                                                                               >  2:975729/1‑237 (MQ=255)

                                                                                          cgcCACCAGCGAGGATCCACGCTTCGGCAGCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGGGTTTTCACCGTGTCGATCCAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGGGACAATCGGTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGGTGCTGAACGTAGAGGagg                                                                                                                                                         >  2:629833/1‑249 (MQ=255)

                                                                                              cccggcgagtacccccGCTTCGGAAGGGGTTGGCAGGTCCGGGTGGGCTTCCCACAGCGCAGTGGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCTCCGTGTCGATACAGTTAACAAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGTGACAATCGCTGGACCGGTTTGGGTATTGAATATCAGTCGGGCAGGATCGTCCTTACCACCAATACTGAGATGCTGAACGTCGAGGATCGGtt                                                                                                                                                     <  2:537598/236‑1 (MQ=255)

                                                                                                    gAGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCtctt                                                                                                                                               >  1:265357/1‑251 (MQ=255)

                                                                                                        aTCCACGCTTCGGAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCtcttctac                                                                                                                                           >  1:837242/1‑249 (MQ=255)

                                                                                                            aCGCTTCGGAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTacggca                                                                                                                                       <  1:401323/251‑2 (MQ=255)

                                                                                                             cGCTTCGGAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGTTCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTacggca                                                                                                                                       >  1:134475/1‑249 (MQ=255)

                                                                                                             cGCTTCGGAAGAAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAACAGACGGTAACGATCGCCGCGAACAATCAGGCTGGCGACAATCGCTGGACCGGGTTGGGCATTGCAGATCAGGCGGGCAGGATCGTCCTTACCCCCAATACCGGGAGGGCGAAGGCCGAGGCTAGGTTTCTCTTCTacggca                                                                                                                                       >  2:502690/1‑249 (MQ=255)

                                                                                                              acTTCGGAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAAAGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGGCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGACGAGGATCGGTTTCTCTTCTacggcca                                                                                                                                      >  2:767148/2‑248 (MQ=255)

                                                                                                                  cGGAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAAT‑CGAc                                                                                                                                <  1:5616/251‑1 (MQ=255)

                                                                                                                   ggAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCTCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAAT‑CGACg                                                                                                                               <  1:62/251‑1 (MQ=255)

                                                                                                                    gAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGTGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAAT‑CGACgg                                                                                                                              <  1:100231/251‑1 (MQ=255)

                                                                                                                    gAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAAT‑CGACgg                                                                                                                              <  1:980760/251‑1 (MQ=255)

                                                                                                                     aaGCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGGGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTCCCACCACTACCGAGCTGCTGAACGTCGGGGATCGGGTTCTCTTTTACGGCAAT‑CGACggg                                                                                                                             >  2:273251/1‑251 (MQ=255)

                                                                                                                     aaGCAGATGGCAGATCCGGTTGAGCATTACACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACCGTTAACCAGCAGACGGACACGATAGCCGAGATCCATCAGGCTGGCGCCAATCGCCGGACAGGTTTGGGGATTGAAGATCCGAGGGGCAGGATGGACCTTACCACCAATACCGAGATGCTGAACGTCGGGGAGAGGTTTCtctttgacgaccaacgacggg                                                                                                                              >  2:937441/1‑234 (MQ=255)

                                                                                                                        cAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAAT‑CGACgagc                                                                                                                            >  1:480552/1‑246 (MQ=255)

                                                                                                                              gCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAAT‑CGACGGGCAGACTTcc                                                                                                                    <  1:255382/251‑1 (MQ=255)

                                                                                                                               cAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCCGTTTCGGCAGGGTGTGCGGTGTTTTAACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGCGATCAATCAGGCTGGCGACAATCCCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAAT‑CGACCGGCCGGCTTccc                                                                                                                   >  2:442615/1‑250 (MQ=255)

                                                                                                                                   tCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAAT‑CGACGGGCAGACTTCCAGCat                                                                                                               >  1:371861/1‑251 (MQ=255)

                                                                                                                                    ccGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAAT‑CGACGGGCAGACTTCCAGCata                                                                                                              <  1:245260/251‑1 (MQ=255)

                                                                                                                                      ggTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCCCCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCCATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGG‑AATACGACGGGCCGACTTCCCGCATATg                                                                                                            >  2:434601/1‑251 (MQ=255)

                                                                                                                                        ttGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAAT‑CGACGGGCAGACTTCCAGCATATggg                                                                                                          >  1:103894/1‑251 (MQ=255)

                                                                                                                                         tGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAAT‑CGACGGGCAGACTTCCAGCATATggg                                                                                                          <  1:752694/250‑1 (MQ=255)

                                                                                                                                         tGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAAT‑CGACGGGCAGACTTCCAGCATATggg                                                                                                          >  1:602681/1‑250 (MQ=255)

                                                                                                                                         tGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTGCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAAT‑CGACGGGCAGACTTCCAGCATATggg                                                                                                          >  1:273665/1‑250 (MQ=255)

                                                                                                                                               ttCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGCGATGCTGAACGTCGCGGATCGGTTTCTCTTCTACGGCCAT‑CGACGGGCAGACTTCCAGCATATGGGTGCCg                                                                                                     >  2:479146/1‑249 (MQ=255)

                                                                                                                                                   acaGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAAT‑CGACGGGCAGACTTCCAGCATATGGGAGCCGAGCAcc                                                                                               <  1:339706/251‑1 (MQ=255)

                                                                                                                                                       cgcATCCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCCCCGTGTCGATACAGTTAACCAGCAGACGGGAACGAGCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATGCCGAGATGCTTAACGTCGAGGATCGGTTTTTCTTCTACGGCAAG‑CGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGt                                                                                           <  2:429794/251‑1 (MQ=255)

                                                                                                                                                        gcATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGGGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGGTCAATCAGGCTGGGGACAAACGCTGTCCCGGGTTGGGTATTGAAGCACAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGGTGCTGTACGGCGAGGAGCGGTTTCTCTTCTACGTCAAT‑CGACGGGCCGACTTCCAGCATATGGGTGTCGAGCACCAgggc                                                                                          >  2:246271/1‑249 (MQ=255)

                                                                                                                                                             cGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAAT‑CGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTa                                                                                      <  2:475848/250‑1 (MQ=255)

                                                                                                                                                                    ggCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCCGTCGGGCCGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAAT‑CGACGGGCAGACTTCCCGCATATGGGGGCCGAGCACCGGGTCCTTACCTTtctc                                                                              >  2:876194/1‑251 (MQ=255)

                                                                                                                                                                      cAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAAT‑CGACGGGCAGACTTCCAGCATATGGGAGCCGCGCACCAGGTCATTACCTTTCTCg                                                                             >  1:703611/1‑250 (MQ=255)

                                                                                                                                                                       aGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCAGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCCCCAATACCGAGATGCTGAACGACGAGGATCGGTTTCTCTTCTACGGCAAT‑CGACGGGCAGACTTCCCGCATATGGGAGCCCAGCACCAGGGCATTCCCTTTCTCgc                                                                            >  2:90047/1‑249 (MQ=255)

                                                                                                                                                                            cGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAAT‑CGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGAt                                                                      <  1:288070/251‑1 (MQ=255)

                                                                                                                                                                                aGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAAT‑CGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGgt                                                                  <  2:461821/251‑1 (MQ=255)

                                                                                                                                                                                    aGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAAT‑CGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAg                                                              <  1:520113/251‑1 (MQ=255)

                                                                                                                                                                                          gtgtttgacccgtttcgagacAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAAT‑CGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGtcctcc                                                        <  2:931356/231‑1 (MQ=255)

                                                                                                                                                                                            gtTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAAT‑CGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGACATTACCTTTCTCGAAGGGATAGGTGTAGTCCTCCa                                                       >  1:707645/1‑250 (MQ=255)

                                                                                                                                                                                                   ccGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAAGCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAAT‑CGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAaggag                                               <  1:325819/251‑1 (MQ=255)

                                                                                                                                                                                                    cGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAAT‑CGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAATCCTCCCTAAaggagg                                              >  2:773408/1‑251 (MQ=255)

                                                                                                                                                                                                       gtCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAAT‑CGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGCGTAGGCCTCCATAAAGGAGGTgc                                           >  1:123366/1‑251 (MQ=255)

                                                                                                                                                                                                                 ttAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAAT‑CGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGc                                   >  1:178277/1‑249 (MQ=255)

                                                                                                                                                                                                                  tAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAAT‑CGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGTTGCCGCCCTGCAg                                 <  1:758493/250‑1 (MQ=255)

                                                                                                                                                                                                                    aCCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAAT‑CGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGAcc                              <  1:410002/251‑1 (MQ=255)

                                                                                                                                                                                                                     ccAGCAGATGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAAT‑CGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCg                             <  1:512931/251‑1 (MQ=255)

                                                                                                                                                                                                                      cagcaGACGGTAACGATCGCCGAGAGCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAAT‑CGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCgg                            <  2:599797/247‑1 (MQ=255)

                                                                                                                                                                                                                       agcaGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAAT‑CGCCGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGt                           >  1:661679/4‑251 (MQ=255)

                                                                                                                                                                                                                       agcaGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAAT‑CGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGt                           <  1:434601/248‑1 (MQ=255)

                                                                                                                                                                                                                       agcaGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAAT‑CGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGt                           >  1:163676/4‑251 (MQ=255)

                                                                                                                                                                                                                        gcaGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAAT‑CGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGtt                          >  1:165893/3‑251 (MQ=255)

                                                                                                                                                                                                                         caGACGGTAACGATCGCCGAGATCAATCAGTCTGGCGACAAGCGCTGGACCGGTTTGCGTATTGACGATCAGTCGGGCAGGATCGTCCTTACCACCTATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAAT‑CGACGGGCAGCCTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTg                         <  1:535077/250‑1 (MQ=255)

                                                                                                                                                                                                                           gACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAAT‑CGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGAc                       <  1:310691/251‑1 (MQ=255)

                                                                                                                                                                                                                              ggTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAAT‑CGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGACATc                    >  1:281574/1‑251 (MQ=255)

                                                                                                                                                                                                                               gTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAAT‑CGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGACATCa                   <  1:695448/251‑1 (MQ=255)

                                                                                                                                                                                                                                 aaCGATCGCCGAGAGCAATCAGGCGGGCGACAAATGCTGGACAGGTTTGGGTAATGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGGCGAGGATCGGGTTCTCTTCTACGGCAAT‑CGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGAAATCAc                  <  2:349276/250‑1 (MQ=255)

                                                                                                                                                                                                                                  aCGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAAT‑CGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGACATCACCt                <  2:172407/251‑1 (MQ=255)

                                                                                                                                                                                                                                    gATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAAT‑CGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGACATCACCTTc              >  1:560790/1‑251 (MQ=255)

                                                                                                                                                                                                                                     aTCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTTCCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAAT‑CGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGACATCACCTTCa             <  1:947662/251‑1 (MQ=255)

                                                                                                                                                                                                                                            agaTCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAAT‑CGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGACATCACCTTCATGATGCg      <  1:175604/251‑1 (MQ=255)

                                                                                                                                                                                                                                             gaTCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAAT‑CGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGACATCACCTTCATGATGCGa     <  1:902378/251‑1 (MQ=255)

                                                                                                                                                                                                                                                caatcagGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAAT‑CGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGGGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGACATCACCTTCATGATGCGAAGc  >  1:342528/1‑251 (MQ=255)

                                                                                                                                                                                                                                                  atcagGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAAT‑CGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGACATCACCTTCATGATGCGAAGc  >  1:870951/1‑249 (MQ=255)

TATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAATTGGCGCATATCGTTGAGGTTCAGTGCATGGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATGCAGTTAACCAGTAGACGGTAACGATCGCCGAGATCAATCAAGCTGGCGACAATCGCTGGGCCGGTTTGGGTATTGAAGATCAGGCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTGCGGCGAT‑CGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGACATCACCTTCATGATGCGAAGC  >  NC_000913/66906‑67396

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`