BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	67,111	G→A	C409C (TGC→TGT)	araA ←	L‑arabinose isomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	67,111	0	G	A	96.0%	443.3 / NA	152	C409C (TGC→TGT)	araA	L‑arabinose isomerase
Reads supporting (aligned to +/- strand): ref base G (2/1); major base A (66/77); minor base C (5/1); total (73/79)
Fisher's exact test for biased strand distribution p-value = 1.03e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 152 total aligned reads displayed.

CCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGTGTGTCGTTATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAATTGGCGCATATCGTTGAGGTTCAGTGCATGGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGA‑TCCACGCTTCGGAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATGCAGTTAACCAGTAGACGGTAACGATCGCCGAGATCAATCAAGCTGGCGACAATCGCTGGGCCGGTTTGGGTATTGAAGATCAGGCGGGCAG‑GATCGTCCTTACCACCAATACCGAG‑ATGCTGAACGTCGAGGATCGGTTTCTCTTCTGCGGCGAT‑CGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCC  >  NC_000913/66865‑67356

ccAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGTATCGTTATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGA‑TCCACGCTTCGGAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAg                                                                                                                                                                                                                                                       <  1:981795/250‑1 (MQ=255)

ccAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGGGTATCGTTATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGA‑TCCACGCTTCGGAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAg                                                                                                                                                                                                                                                       <  1:691235/250‑1 (MQ=255)

  agcgcagcgcGTCTTTAAACGCTGGCAGGCGGGTATCGTTATCAATCCCCGGGCTTTCAATGTCGTGCCTCTCTGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATTGCTGAAGGAGGTTTGGATCGCGCCACCAGCCAGGGGTCC‑CGCTTTGGGAGCAGTTGGCCGATCCGGTTGCGCTTTTCACAGCGCATTTCCCACCGGCAGGTTCGGCAGGGAGGGCGGGGTTTTTTCCGTGTCGGTGCactgt                                                                                                                                                                                                                                                    >  2:963081/1‑247 (MQ=255)

   gcgcagcgcGTCTTTAAACGCTGGCAGGCGGGTATCGTTATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGCTGAAGACGGTATGGTGCGCGCCCCCAGCGAGGA‑TCCACGCTTCGGAAGCAGTTGGCAGAGCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTaa                                                                                                                                                                                                                                                   <  2:504026/251‑1 (MQ=255)

      cagcgcGTCTTTAAACGCTGGCAGGCGGGTATCGTTATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGA‑TCCACGCTTCGGAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCa                                                                                                                                                                                                                                                <  2:454029/251‑1 (MQ=255)

      cagcgcGTCTTTAAACGCTGGCAGGCGGGTATCGTTATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGA‑TCCACGCTTCGGAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCa                                                                                                                                                                                                                                                <  1:741446/251‑1 (MQ=255)

      cagcgcGTCTTTAAACGCTGGCAGGCGGGGATCGTTATCAATCACCGTGATTTCAATGTCGGGCATCTCGGCGAACTGGCGCCTATCGTTGAGGTTCAGCGCATGGCTGAAGACGGTATGGGGCGCGCCACCCGCGAGGG‑TCCACGCTTCGGAAGCAGTTGGCAGATCCGGGTGCGCTTTCCCCAGCGCCTTCGCCCCCGGCAGGTTCGGCAGGGGGGGCGGGGTTTTTACCCTGTCGGAACAGTTAAcc                                                                                                                                                                                                                                                 >  2:339706/1‑250 (MQ=255)

          gcgTCTTTAAACGCTGGCAGGCGGGTATCGTTATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGA‑TCCACGCTTCGGAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAgcag                                                                                                                                                                                                                                            <  1:652328/251‑4 (MQ=255)

                tgaaaCGCTGGCAGGCGGGTATCGTGATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGA‑TCCACGCTTCGGAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACgg                                                                                                                                                                                                                                        <  1:2236/247‑1 (MQ=255)

                  aaactcgggCAGGCGGGTATCTTTATCAATCACCTGGGTTTCAATGTCGTGCATCTCGGCGAATTGGCGCATATCGTTGAGGTTCAGCGCATGGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGA‑TCCACGCTTCGGAAGCAGTTGGCAGAGCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTaa                                                                                                                                                                                                                                     <  2:513033/243‑1 (MQ=255)

                  aaaCGCTGGCAGGCGGGTATCGTTATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGA‑TCCACGCTTCGGAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAAc                                                                                                                                                                                                                                    <  1:381576/251‑1 (MQ=255)

                  aaaCGCTGGCAGGCGGGGATCGGTATCAATCACCGAGATTTCAATGTCGTGCATCTCGGCGAACTGGCGCAAATCGTTGAGGTTCAGCGCATGGCTGAAGACGGTATGGTGCGCGCCACCAGCGGGGA‑TCCACGCTTCGGAAGCAGTTGGCAGATCCGGGTGCGCTTTCCCCAGCGCATTCGCCACCGGCAGGTTCGGCAGGGGGGGGGGGGGTTTTACCGTGTCGATAAAGGTAACCAGCAGAAGGTaa                                                                                                                                                                                                                                     >  1:389155/1‑250 (MQ=255)

                      gCTGGCAGGCGGGTATCGTTATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGA‑TCCACGCTTCGGAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATc                                                                                                                                                                                                                                <  1:285187/251‑1 (MQ=255)

                             ggcgggtatCGTTATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGA‑TCCACGCTTCGGAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGAc                                                                                                                                                                                                                                          <  1:611677/226‑1 (MQ=255)

                              gcgggtatCGTTATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGA‑TCCACGCTTCGGAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCg                                                                                                                                                                                                                            >  1:258063/8‑247 (MQ=255)

                               cgggtatCGTTATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGA‑TCCACGCTTCGGAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGatc                                                                                                                                                                                                                       <  1:404430/245‑1 (MQ=255)

                                    atCGTTATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGA‑TCCACGCTTCGGAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGatcaatc                                                                                                                                                                                                                   >  1:829869/2‑250 (MQ=255)

                                    atCGTTATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAACTGGCGCATATCGTCGAGGTTCAGCGCCTCGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGA‑TCCACGCTTCGGAAGCCGTTGGCAGATCCGGTTGCGATTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGCGCGGTGTTTTCACCGTATCGATACAGTTACACAACAGACGGTAACGATCGCCGAGatcaatc                                                                                                                                                                                                                   >  1:589125/2‑250 (MQ=255)

                                      cGTTATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGA‑TCCACGCTTCGGAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGCGTGCGGTGTTTTAACCGTGTCGGACCAGTTAACCAGCAGACGGAACCGGTCGCCATGatcaatcagg                                                                                                                                                                                                                >  2:829669/1‑249 (MQ=255)

                                      cGTTATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGA‑TCCACGCTTCGGAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGatcaatcagg                                                                                                                                                                                                                <  1:489726/251‑3 (MQ=255)

                                      cGTTATCAATCACCGTGATTTCAATGTCGTGCATCGCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGA‑TCCACGCTTCGGAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGagcaatcag                                                                                                                                                                                                                 <  2:391553/250‑2 (MQ=255)

                                         tATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGA‑TCCACGCTTCGGAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCt                                                                                                                                                                                                              <  1:365829/250‑1 (MQ=255)

                                             aatcaCCGTGATT‑CAATGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGCTGAAGACGGTATGGGGCGCGCCACCAGCGAGGA‑TCCACGCTTCGGAAGCAGTTGGCAGATCCGGGTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGGTAACCAGCAGAAGGTAACGATCGCCGAGAGCAATCAGGCTGGCGAc                                                                                                                                                                                                        >  1:655792/1‑251 (MQ=255)

                                                caCCGTGATTTCAATGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGA‑TCCACGCTTCGGAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACa                                                                                                                                                                                                       <  2:120580/250‑1 (MQ=255)

                                                          tcattgtctttcATCCCGGCGCACTGGCGCATATCGTTGATGTCCAGCGCATGGCTGAAGATGGTATGGGTCGCGCCCCCATCGAGGA‑TCCACGCTTCGGAAGCAGTTGGAAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCCCCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGCCGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTggac                                                                                                                                                                                             <  2:751383/239‑3 (MQ=255)

                                                          tCAATGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGA‑TCCACGCTTCGGAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCCGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTggacc                                                                                                                                                                                            >  1:525423/1‑248 (MQ=255)

                                                          tCAATGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGA‑TCCACGCTTCGGAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTggacc                                                                                                                                                                                            >  1:709733/1‑248 (MQ=255)

                                                           cAATGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGA‑TCCACGCTTCGGAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTggacc                                                                                                                                                                                            <  1:145590/250‑4 (MQ=255)

                                                            aaTGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGA‑TCCACGCTTCGGAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCgg                                                                                                                                                                                          >  1:955330/1‑251 (MQ=255)

                                                             aTGTCGTGCATCTCGGCAAACTGGCGAATATCGTTGAGGTTAAGCGCATGGCTGAAGACGGTATGGAGCGCGCCACCAGCGAGGA‑TCCACGCTTCGGAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATCCAGTTAACCAGCAGACGGTACAGATCGCCGAGATCACTCAGGCTGGCGCCACTCGCTGGACCggg                                                                                                                                                                                         >  2:630943/1‑250 (MQ=255)

                                                               ggcGTGCATCGCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGA‑TCCACGCTTCGGAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGtt                                                                                                                                                                                        <  2:709733/248‑1 (MQ=255)

                                                                 cGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCGTGGCTGAAGACGGTATGGTGAGCGCCACCAGCGAGGA‑TACACGCTTAGGAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGCTACAGGTAACCAGCAGACGGTAACGATCGCCGCGATAAATCAGGATGGCGACAATCGCTGGACCGGTTTgg                                                                                                                                                                                     >  1:586180/1‑251 (MQ=255)

                                                                  gggCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGAATGTCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGA‑TCCACGCTTCGGAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTggg                                                                                                                                                                                    <  2:612003/249‑1 (MQ=255)

                                                                  gTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGA‑TCCACGCTTCGGAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTggg                                                                                                                                                                                    >  1:931356/1‑251 (MQ=255)

                                                                   ggCATCGCGGCGAAGTGGCGCATATCGTTGATGGTCAGCGCATGGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGA‑GCCTCGCTTCGGAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTGCGGCAGGGAGGGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGAGCGCCGAGAGCAATCAGGCTGGCGACAATCGCTGGATCGGTGTGGGt                                                                                                                                                                                   <  2:168354/250‑1 (MQ=255)

                                                                          cGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGA‑TCCACGCGTCGGAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCATGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAg                                                                                                                                                                            <  1:36119/251‑1 (MQ=255)

                                                                          cGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGCTGAAGACGGTATGGGGCGCGCCACCAGCGAGGA‑TCCACGCTTCGGAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGaa                                                                                                                                                                             <  2:369577/250‑1 (MQ=255)

                                                                           ggCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGA‑TCCACGCTTCGGAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGa                                                                                                                                                                              >  1:891850/1‑248 (MQ=255)

                                                                                actGGCGCATATCGTTGAGGGTCAGCGGATGGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGA‑TCCACGCATCGGAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTGCGGCAGGGAGTGCGTTGTTTTCACCGTGTCGATACAGTTACCCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAgt                                                                                                                                                                      <  1:892071/249‑2 (MQ=255)

                                                                                  tGGCACATATCGTTGAGGTTCAGCGCATGGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGA‑TCCACGCTTCGGAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGGGCGGTGTTTTCACCGTGGCGATACAGTTAACCAGCAGACGGTGACGATCGCCGAGAACAATCCGGCTTGCGACAATACCCGGACCGGATTGGGAATTGAAAATCAgacg                                                                                                                                                                    >  2:893266/1‑248 (MQ=255)

                                                                                        atatCGTTGAGGTTCAGCGCATGGCTGAAGACGGTATGGTGCGCGCCCCCAGCGAGGA‑TCCACGCTTCGGAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCACATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAg‑g                                                                                                                                                             <  2:217463/251‑1 (MQ=255)

                                                                                              ttGAGGTTCAGCGCATGGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGA‑TCCACGCTTCGGAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGTGCGCGGTGTTTTCACCGTGTCGATCCCGTTAACCAGCAGACGGTAACGATCGCCGCGATCAATCAGGCCGGCGAACATCGCTGGACCGGTTTGGGTAATGAAGATCAAGCGGGGAg‑gcacggc                                                                                                                                                       >  2:362259/1‑245 (MQ=255)

                                                                                                 aGGTTCAGCGCATGGCTGAAGAGGGTATGGTGCGCGCCACCATCGAGGA‑TCCACGCTTCGGAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATCCAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAG‑GATCGTc                                                                                                                                                       <  1:211415/248‑1 (MQ=255)

                                                                                                       cgcgCATGGCTGAAGCCGGTATGGTGCGCGCCTCCAGCGAGGA‑TCCACGCTTCGGAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACTGGGTCGATACAGTTATCCAGCAGACGGTAACTATCGCCGAGATCAATCAGGCTGGCGACAATCTCTGGACCTGTTTGGGTATTGAAGATCAGTCGGGCAG‑GATCGTCCTTaccacc                                                                                                                                              <  1:877953/248‑1 (MQ=255)

                                                                                                       agcgCATGGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGA‑TCCACGCTTCGGAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAG‑GATCGTCCTTaccacc                                                                                                                                              >  1:397858/4‑251 (MQ=255)

                                                                                                                cTGAAGACGGTATGGTGCGCGCCACCAGCGAGGA‑TCCACGCTTCGGAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAG‑GATCGTCCTTACCACCAATACCg                                                                                                                                       >  1:625742/1‑249 (MQ=255)

                                                                                                                  gAAGACGGTATGGTGCGCGCCACCAGCGAGGA‑TCCACGCTTCGGAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTGCGGCAGGGAGTGCGGGGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCACACAGGCTGGCGCCAATCGCTGGACCGGATTGGGGATTGAAGATCAGTCGGGCAG‑GAGCGTCCTTaccacctcaccccaggt                                                                                                                                   >  2:172271/1‑240 (MQ=255)

                                                                                                                   aaGACGGTATGGTGCGCGCCACCAGCGAGGA‑TCCACGCTTCGGAAGCAGTTGGCAGCTCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGGTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAG‑GATCGTCCTTACCACCAATACCGAG‑ATg                                                                                                                                 <  1:475929/251‑1 (MQ=255)

                                                                                                                     gACGGTATGGGGCGCGCCACCAGCGAGGA‑TCCACGCTTCGGAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGAGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGGATTGAAGATCAGTCGGGCAG‑GGTCGTCCTTACCACCAATACCGAG‑ATGCt                                                                                                                               >  1:659178/1‑251 (MQ=255)

                                                                                                                         gTATGGTGCGCGCCACCAGCGAGGA‑TCCACGCTTCGGAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAG‑GATCGTCCTTACCACCAATACCGAG‑ATGCTGAAc                                                                                                                           <  1:141894/251‑1 (MQ=255)

                                                                                                                         gTATGGTGCGCGCCACCAGCGAGGA‑TCCACGCTTCGGAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAG‑GATCGTCCTTACCACCAATACCGAG‑ATGCTGAAc                                                                                                                           <  1:930553/251‑1 (MQ=255)

                                                                                                                          aaTGGTGCGCGCCACCAGCGAGGA‑TCCACGCTTCGGAATCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGGTTTCACCGTGTCGATACAGTTAAACCGCAGACAGTAACGAACGCCGACATCAAACAGGCTTGCGACAATCGCTGGACCGGTTTTGGAATTGCAATTCAAGCGGGCAG‑GATCGTCCTTACCACCAATAcccagcagctgtacg                                                                                                                           >  2:250635/2‑238 (MQ=255)

                                                                                                                           aTGGTGCGCGCCACCAGCGAGGA‑TCCACGCTTCGGAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCCGGGGGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGAACGCCGAGATCAATCAGGCTGGCGCCAATCGCTGGCCAGGTTTGGGTATTGAAGATCAATCGGGCAG‑GATAGTCCTTACCACCaaacacgaggttctgaacgt                                                                                                                          >  2:835668/1‑233 (MQ=255)

                                                                                                                             ggTGCGCTGCACCATCGAGGA‑GCCCCGCTTCGGAAGCAGTTGGCAGATCCGGTTGCGCTTCCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGGTCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGAACATTCGGGCAG‑GATCGTCCTTACCGCCAATACCGAG‑ATGCTGAACGTc                                                                                                                        <  2:844684/250‑1 (MQ=255)

                                                                                                                             ggTGCGCGCCACCAGCGAGGA‑TCCACGCTTCGGAAGCAGTTGGCAGAGCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAG‑GATCGTCCTTACCACCAATACCGAG‑ATGCTGAACGTCg                                                                                                                       <  2:306699/251‑1 (MQ=255)

                                                                                                                              gTGCGCGCCCCCAGGTAGGA‑TCCACGCTTAGGAAGCAGTTGGCAGCTGCGGTTGCGCTGTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTGTTCACCGTGACGATACAGTGAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCGGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAG‑GATCGTTCTTATCACCAATTCCGAG‑ATGCTGAACGTCGa                                                                                                                      <  2:1233/251‑1 (MQ=255)

                                                                                                                              gTGCGCGCCACCAGCGAGGA‑TCCACGCTTCGGAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGCGTTTTCACCGTGTCGATACAGTTAACCATCAGAAGGTAACGATCGCCGAGATCCATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGAGCAGTCGGGCGG‑GGTCGTCCTTACCACCAAAACCGCG‑ATGCAGTACGTCgg                                                                                                                      >  2:467220/1‑250 (MQ=255)

                                                                                                                               tGCGCGCCACCAGCGAGGA‑TCCACGCTTAGGAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCCGACGGTAACGATCGACGCGATCACTCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGACCCATCGGGCCG‑GATCGTCCTTACCAACAATACCcag‑agcctggacatcg                                                                                                                       >  2:975729/1‑237 (MQ=255)

                                                                                                                                   cgcCACCAGCGAGGA‑TCCACGCTTCGGAAGCAGTTGGCGGATCCGGTTGCGTTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGCTCAGTCggg                                                                                                                                                                  <  2:675938/204‑1 (MQ=255)

                                                                                                                                   cgcCACCAGCGAGGA‑TCCACGCTTCGGAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACAATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGATTCGGTATTGAAGCTCAGTCggg                                                                                                                                                                  >  1:675938/1‑204 (MQ=255)

                                                                                                                                        cccggcgagtacccccGCTTCGGAAGGGGTTGGCAGGTCCGGGTGGGCTTCCCACAGCGCAGTGGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCTCCGTGTCGATACAGTTAACAAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGTGACAATCGCTGGACCGGTTTGGGTATTGAATATCAGTCGGGCAG‑GATCGTCCTTACCACCAATACTGAG‑ATGCTGAACGTCGAGGATCGGtt                                                                                                             <  2:537598/236‑1 (MQ=255)

                                                                                                                                             gAGGA‑TCCACGCTTCGGAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAG‑GATCGTCCTTACCACCAATACCGAG‑ATGCTGAACGTCGAGGATCGGTTTCtctt                                                                                                       >  1:265357/1‑251 (MQ=255)

                                                                                                                                                 a‑TCCACGCTTCGGAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAG‑GATCGTCCTTACCACCAATACCGAG‑ATGCTGAACGTCGAGGATCGGTTTCtcttctac                                                                                                   >  1:837242/1‑249 (MQ=255)

                                                                                                                                                   cccACGCTTCGGAAGCAGTTTGCAGTGCCGGTTGCGCTTTCCACAGCGCATGCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTTGACCGGTTTGGGTATTGAAGATCAGTCGGGCAG‑GATCGTCCTTACCACCAATACCGAG‑ATGCTGAACGTCGAGGATCGGTTTCtcttctacg                                                                                                  <  2:332450/250‑4 (MQ=255)

                                                                                                                                                      aCGCTTCGGAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAG‑GATCGTCCTTACCACCAATACCGAG‑ATGCTGAACGTCGAGGATCGGTTTCTCTTCTacggca                                                                                               <  1:401323/251‑2 (MQ=255)

                                                                                                                                                       cGCTTCGGAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGTTCAGTCGGGCAG‑GATCGTCCTTACCACCAATACCGAG‑ATGCTGAACGTCGAGGATCGGTTTCTCTTCTacggca                                                                                               >  1:134475/1‑249 (MQ=255)

                                                                                                                                                       cGCTTCGGAAGAAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAACAGACGGTAACGATCGCCGCGAACAATCAGGCTGGCGACAATCGCTGGACCGGGTTGGGCATTGCAGATCAGGCGGGCAG‑GATCGTCCTTACCCCCAATACCGGG‑AGGGCGAAGGCCGAGGCTAGGTTTCTCTTCTacggca                                                                                               >  2:502690/1‑249 (MQ=255)

                                                                                                                                                            cGGAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAG‑GATCGTCCTTACCACCAATACCGAG‑ATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAAT‑CGAc                                                                                        <  1:5616/251‑1 (MQ=255)

                                                                                                                                                              gAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGCGATCAATCAGGCTGGCGCCAATCGCTGGACCGGTTTGGGTATTGACGATCAGTCGGGCAG‑GATCGTCCTTACCACCAATACCGCG‑ATGCTGAACGTCGAGGGGCGGTTTCTCTTCTacggcaaccgaccg                                                                                       >  2:205441/1‑242 (MQ=255)

                                                                                                                                                              gAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGTGCAG‑GATCGTCCTTACCACCAATACCGAG‑ATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAAT‑CGACgg                                                                                      <  1:100231/251‑1 (MQ=255)

                                                                                                                                                              gAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAG‑GATCGTCCTTACCACCAATACCGAG‑ATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAAT‑CGACgg                                                                                      <  1:980760/251‑1 (MQ=255)

                                                                                                                                                               aaGCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGGGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAG‑GATCGTCCTTCCCACCACTACCGAG‑CTGCTGAACGTCGGGGATCGGGTTCTCTTTTACGGCAAT‑CGACggg                                                                                     >  2:273251/1‑251 (MQ=255)

                                                                                                                                                               aaGCAGATGGCAGATCCGGTTGAGCATTACACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACCGTTAACCAGCAGACGGACACGATAGCCGAGATCCATCAGGCTGGCGCCAATCGCCGGACAGGTTTGGGGATTGAAGATCCGAGGGGCAG‑GATGGACCTTACCACCAATACCGAG‑ATGCTGAACGTCGGGGAGAGGTTTCtctttgacgaccaacgacggg                                                                                      >  2:937441/1‑234 (MQ=255)

                                                                                                                                                                   aGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTTTCGATGCAGTTAACCAGTAGACGGTAACGGTCGCCGAGATCCATCCAGCTGGCGACAATCGCTGGGCCGGTTTGGGTATTTAAGATCCGGCGGG‑AGAGGTCGTCCTTACCCCCAATCCCGAGCAT‑CTGCACGTCGAGGGTCTGTTTCTCTTCTtcggcggaccaccggc                                                                                     >  2:81136/1‑238 (MQ=255)

                                                                                                                                                                    gTTGGCAGATCCGGTTGCGCGTTCCACAGCGCAGTCGCCACCGGCATTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAG‑GATCGTCCTTACCACCAATACCGAG‑ATGCTGAACGTCGAGGAGCGGTTTCTCTTCTACGGCAAT‑CGACGGGCAGAc                                                                                <  2:629216/251‑1 (MQ=255)

                                                                                                                                                                       ggCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAG‑GATCGTCCTTACCACCAATACCGAG‑ATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAAT‑CGACGGGCAGACTTc                                                                             >  1:291182/1‑251 (MQ=255)

                                                                                                                                                                        gCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAG‑GATCGTCCTTACCACCAATACCGAG‑ATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAAT‑CGACGGGCAGACTTcc                                                                            <  1:255382/251‑1 (MQ=255)

                                                                                                                                                                         cAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCCGTTTCGGCAGGGTGTGCGGTGTTTTAACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGCGATCAATCAGGCTGGCGACAATCCCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAG‑GATCGTCCTTACCACCAATACCGAG‑ATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAAT‑CGACCGGCCGGCTTccc                                                                           >  2:442615/1‑250 (MQ=255)

                                                                                                                                                                              ccGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAG‑GATCGTCCTTACCACCAATACCGAG‑ATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAAT‑CGACGGGCAGACTTCCAGCata                                                                      <  1:245260/251‑1 (MQ=255)

                                                                                                                                                                               cGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAG‑GATCGTCCTTACCACCAATACCGAG‑ATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAAT‑CGACGGGCAGACTTCCAGCatat                                                                     <  1:167525/251‑1 (MQ=255)

                                                                                                                                                                                ggTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCCCCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAG‑GATCGTCCTTACCACCCATACCGAG‑ATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGG‑AATACGACGGGCCGACTTCCCGCATATg                                                                    >  2:434601/1‑251 (MQ=255)

                                                                                                                                                                                  ttGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAG‑GATCGTCCTTACCACCAATACCGAG‑ATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAAT‑CGACGGGCAGACTTCCAGCATATggg                                                                  >  1:103894/1‑251 (MQ=255)

                                                                                                                                                                                   tGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAG‑GATCGTCCTTACCACCAATACCGAG‑ATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAAT‑CGACGGGCAGACTTCCAGCATATggg                                                                  >  1:602681/1‑250 (MQ=255)

                                                                                                                                                                                   tGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAG‑GATCGTCCTTACCACCAATACCGAG‑ATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAAT‑CGACGGGCAGACTTCCAGCATATggg                                                                  <  1:752694/250‑1 (MQ=255)

                                                                                                                                                                                                  gcATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGGGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGGTCAATCAGGCTGGGGACAAACGCTGTCCCGGGTTGGGTATTGAAGCACAGTCGGGCAG‑GATCGTCCTTACCACCAATACCGAG‑GTGCTGTACGGCGAGGAGCGGTTTCTCTTCTACGTCAAT‑CGACGGGCCGACTTCCAGCATATGGGTGTCGAGCACCAgggc                                                  >  2:246271/1‑249 (MQ=255)

                                                                                                                                                                                                   cATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAG‑GATCGTCCTTACCACCAATACCGCG‑ATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAAT‑CGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTc                                                  >  1:523458/1‑250 (MQ=255)

                                                                                                                                                                                                     ttCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAG‑GATCGTCCTTACCACCAATACCGAG‑ATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAAT‑CGACGGGCAGCCTTCCAGCATATGGGAGCCGAGCACCAGGTCAtt                                               >  1:181715/1‑251 (MQ=255)

                                                                                                                                                                                                      tCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAG‑GATCGTCCTTACCACCAATACCGAG‑ATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAAT‑CGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCAtt                                               <  1:55582/250‑1 (MQ=255)

                                                                                                                                                                                                       cGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAG‑GATCGTCCTTACCACCAATACCGAG‑ATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAAT‑CGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTa                                              <  2:475848/250‑1 (MQ=255)

                                                                                                                                                                                                         ccACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAG‑GATCGTCCTTACCACCAATACCGAG‑ATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAAT‑CGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCt                                           <  1:274558/251‑1 (MQ=255)

                                                                                                                                                                                                           aCCGGCAGTTTCGGCAGGGAGGTCGGTGTTTTCACCGTTTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGCGATCAATCAGGCTGGCGACAATCGCTGGACCGGGTTGGGTATTGAAGATCAGTCGGGCAG‑GCTCGTCCTTACCACCAATACCGAG‑ATGCTGAACGTCGAGGATCGGATTCTCTTCTACGGCAAT‑CGCCGGGCAGAATTCCAGCATATGGGGGCCGAGCACCCGGTCATTACCttt                                         >  2:413107/1‑251 (MQ=255)

                                                                                                                                                                                                              ggCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCCGTCGGGCCG‑GATCGTCCTTACCACCAATACCGAG‑ATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAAT‑CGACGGGCAGACTTCCCGCATATGGGGGCCGAGCACCGGGTCCTTACCTTtctc                                      >  2:876194/1‑251 (MQ=255)

                                                                                                                                                                                                                 aGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCAGTTTGGGTATTGAAGATCAGTCGGGCAG‑GATCGTCCTTACCCCCAATACCGAG‑ATGCTGAACGACGAGGATCGGTTTCTCTTCTACGGCAAT‑CGACGGGCAGACTTCCCGCATATGGGAGCCCAGCACCAGGGCATTCCCTTTCTCgc                                    >  2:90047/1‑249 (MQ=255)

                                                                                                                                                                                                                  gTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAG‑GATCGTCCTTACCACCAATACCGAG‑ATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAAT‑CGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGa                                    >  1:486413/1‑249 (MQ=255)

                                                                                                                                                                                                                          aGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAAGCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAG‑GATCGTCCGTACCACCAATACCGAG‑ATGCTGAACGTCGAGGATCGGTTTCTCTGCTACGGCAAT‑CGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGgt                          <  1:47169/251‑1 (MQ=255)

                                                                                                                                                                                                                                      gtTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAG‑GATCGTCCTTACCACCAATACCGAG‑ATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAAT‑CGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGACATTACCTTTCTCGAAGGGATAGGTGTAGTCCTCCa               >  1:707645/1‑250 (MQ=255)

                                                                                                                                                                                                                                             ccGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAAGCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAG‑GATCGTCCTTACCACCAATACCGAG‑ATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAAT‑CGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAaggag       <  1:325819/251‑1 (MQ=255)

                                                                                                                                                                                                                                              cGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAG‑GATCGTCCTTACCACCAATACCGAG‑ATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAAT‑CGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAATCCTCCCTAAaggagg      >  2:773408/1‑251 (MQ=255)

                                                                                                                                                                                                                                                tgtCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAG‑GATCGTCCTTACCACCAATACCGAG‑ATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAAT‑CGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGCGGTg    >  1:97405/1‑251 (MQ=255)

                                                                                                                                                                                                                                                 gtCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAG‑GATCGTCCTTACCACCAATACCGAG‑ATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAAT‑CGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGCGTAGGCCTCCATAAAGGAGGTgc   >  1:123366/1‑251 (MQ=255)

CCAGCGCAGCGCGTCTTTAAACGCTGGCAGGCGTGTGTCGTTATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAATTGGCGCATATCGTTGAGGTTCAGTGCATGGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGA‑TCCACGCTTCGGAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATGCAGTTAACCAGTAGACGGTAACGATCGCCGAGATCAATCAAGCTGGCGACAATCGCTGGGCCGGTTTGGGTATTGAAGATCAGGCGGGCAG‑GATCGTCCTTACCACCAATACCGAG‑ATGCTGAACGTCGAGGATCGGTTTCTCTTCTGCGGCGAT‑CGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCC  >  NC_000913/66865‑67356

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`