BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	66,901	G→A	D479D (GAC→GAT)	araA ←	L‑arabinose isomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	66,901	0	G	A	98.4%	321.3 / NA	129	D479D (GAC→GAT)	araA	L‑arabinose isomerase
Reads supporting (aligned to +/- strand): ref base G (0/2); major base A (44/79); minor base C (2/0); total (48/81)
Fisher's exact test for biased strand distribution p-value = 1.34e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.39e-01

Only 100 of 129 total aligned reads displayed.

CTTTGCTTATCCGGCCTACAAAATCGCAGCGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCAGATAAGACGCGTCAGCGTCGCATCAGGCGTTACATACCGGATGCGGCTACTTAGCGACGAAACCCGTAATACACTTCGTTCCA‑GCGCAGCGCGTCTT‑TAAACGCTGGCAGGCGTGTGTCGTTATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAATTGGCGCATATCGTTGAGGTTCAGTGCATGGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGA‑TCCACGCTTCGGAA‑GCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATGCAGTTAACCAGTAGACGGTAACGATC  >  NC_000913/66660‑67137

cTTTGCTTATCCGGCCTACAAAATCGGAACGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCA‑GCGCAGCGCGTCTT‑TAAACGCTGGCAGGCGGGGATCGTTatc                                                                                                                                                                                                                                        >  1:372730/1‑250 (MQ=18)

  ttGCTGATCCGGCCTACTAAATCGGAACGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAAGCGCAGCGTGTAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTGCCA‑GCGCAGCGCGTCTT‑TAAACGCTGGCAGGCGGGTATCGTTatca                                                                                                                                                                                                                                       <  2:143570/249‑1 (MQ=18)

    gCTTATCCGGCCTACAAAATCGGAACGCGTAGGCCTGATAAGACGCGCCAGCGGCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCA‑GCGCAGCGCGTCTT‑TAAACGCTGGCAGGCGGGTATCGTTatcaatca                                                                                                                                                                                                                                   <  1:177175/251‑1 (MQ=21)

    gCTTAGCCGGCCTACAAAATCGGAACGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCA‑GCGCAGCGCGTCTT‑TAAACGCTGGCAGGCGGGTATCGTTatcaatca                                                                                                                                                                                                                                   <  1:600919/251‑1 (MQ=21)

      ttATCCGGCCTACAAAATCGGAACGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCA‑GCGCAGCGCGTCTT‑TAAACGCTGGCAGGCGGGGAACGTTATCAATCAcc                                                                                                                                                                                                                                 >  1:158268/1‑251 (MQ=18)

       tcaccgtccagcAAAATCGGAACGCGTAGGCCTGCTTAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATTCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTGAGCGACGAAATCCGTAATTCACTTCGTTCCA‑GCGCCGCGCGTCTT‑TAAACGCTGTCAGGCGGGTATCGTTATCAATCAc                                                                                                                                                                                                                                  <  2:67473/238‑1 (MQ=18)

         cccGGCCTACAAAATCGGAACGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCA‑GCGCAGCGCGTCTT‑TAAACGCTGGCAGGCGGGTATCGTTATCAATCACCGTg                                                                                                                                                                                                                              <  1:737221/250‑1 (MQ=21)

                 aCAAAATCGGAACGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCA‑GCGCAGCGCGTCTT‑TAAACGCTGGCAGGCGGGTATCGTTATCAATCACCGTGATTTCaa                                                                                                                                                                                                                       <  1:575701/250‑1 (MQ=21)

                            acgcgTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCA‑GCGCAGCGCGTCTT‑TAAACGCTGGCAGGCGGGTATCGTTATCAATCACCGTGATTTCAATGTCGTGCAtct                                                                                                                                                                                                           <  1:738691/250‑1 (MQ=25)

                              gcgTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCA‑GCGCAGCGCGTCTT‑TAAACGCTGGCAGGCGGGTATCGTTATCAATCACCGTGATTTCAATGTCGTGCATCTCg                                                                                                                                                                                                         <  1:172253/251‑1 (MQ=25)

                                  aGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCA‑GCGCAGCGCGTCTT‑TAAACGCTGGCAGGCGGGTATCGTTATCAATCACCGTGATTTCAATGTCGGGCCTCTCGGCg                                                                                                                                                                                                      >  1:611681/1‑250 (MQ=25)

                                        gtttaGACGAGCTAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCA‑GCGCAGCGCGTCTT‑TAAACGCTGGCAGGCGGGTATCGGTATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAACTGgc                                                                                                                                                                                               <  2:704827/247‑1 (MQ=25)

                                         aTAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCA‑GCGCAGCGCGTCTT‑TAAACGCTGGCAGGCGGGTATCGTTATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAACTGgcg                                                                                                                                                                                              <  1:47930/251‑1 (MQ=25)

                                         aTAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCA‑GCGCAGCGCGTCTT‑TAAACGCTGGCAGGCGGGTATCGTTATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAACTGgcg                                                                                                                                                                                              <  1:459363/251‑1 (MQ=25)

                                         aTAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCA‑GCGCAGCGCGTCTT‑TAAACGCTGGCAGGCGGGTATCGTTATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAACTGgcg                                                                                                                                                                                              <  1:621931/251‑1 (MQ=25)

                                                  gcCAGCGTCGCATCAGGCGTTGAATGCCGGCTGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGAGTAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCA‑GCGCCGCGCGTCTT‑TAAACGCTGGCAGGCGGGGATCGTTATCAATCACCGTACTTTTCATGGCGTGCATATCGGCGGAATGGCGCATCTCGtt                                                                                                                                                                                     >  1:756626/1‑251 (MQ=18)

                                                  gcCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCA‑GCGCAGCGCGTCTT‑TAAACGCTGGCAGGCGGGTATCGTTATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAACTGGCGCATATCGtt                                                                                                                                                                                     <  1:693596/251‑1 (MQ=37)

                                                     ctcttcgcctcctgCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGCCGAAATCCGTAATACACTTCGTTCCA‑GCGCAGCGCGTCTT‑TAAACGCTGGCAGGCGGGTATCGTTTTCCATCCCCGTGATTTCCATGTCGTGCATCTCGGCGAACTGGCGCATATCCTTggg                                                                                                                                                                                  >  1:168354/14‑249 (MQ=25)

                                                      gCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCA‑GCGCAGCGCGTCTT‑TAAACGCTGGCAGGCGGGTATCGTTATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAgg                                                                                                                                                                                 <  1:414524/251‑1 (MQ=37)

                                                       cGTCGCATCTGGCGTTGAAGTCCGGATGCGCTTTGCGTTTCCGGTCTACCAAAGCGCAGCGTGTAGGGCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACATCTGATGCGACTACGTAGCGACGAAATCCGTAATACACTTCGTTCCA‑GCTCAGAGCGTCTT‑TAAACGCTGGCAGGCGGGTAGCGTTATCAATCACCGTGATTTCAATGTCGGGCATCTCGGCGAACTGGCGCATATCGTTGAGGt                                                                                                                                                                                <  2:208428/251‑1 (MQ=255)

                                                        gTCGCATCAGGCGTTGAATGCCGGATGCGCTTTTCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCA‑GCGCAGCGCGTCTT‑TAAACGCTGGCAGGCGGGGATCGTTATCAATCAACGTGATTTCAATGTCGTGCATCTCGGCGAAATGGCGCCAATCGTTGAGGtt                                                                                                                                                                               >  1:217463/1‑251 (MQ=25)

                                                            cATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCA‑GCGCAGCGCGTCTT‑TAAACGCTGGCAGGCGGGGATCGGTATAAATCACAGTGGTTTCAATGTAGTGCATCTCGGCGAAATGGGGCATATCGGTGAGGttaagg                                                                                                                                                                           >  1:844684/1‑247 (MQ=21)

                                                                      ttgaggCCGGATGCGCTTTGCGTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGTCAGCGTCGCATCATGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCA‑GCGCAGCGCGTCTT‑TAAACGCTGGCAGGCGGGTATCGTGATCAATCACCGTGATTTCAAGGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGCTg                                                                                                                                                                  <  2:440771/245‑1 (MQ=37)

                                                                        aaTGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGCCGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCA‑GCGCAGCGCGTCTT‑TAAACGCTGGCAGGCGGGGTTAGTTATAAAACACCGGGGTTTCAATGTTGTGTATCTCGGCGCACTGGCGCCTATCCTTTAGGTGACGTGCATGGccgcc                                                                                                                                                                >  2:601392/1‑246 (MQ=16)

                                                                         aTGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCA‑GCGCAGCGCGTCTT‑TAAACGCTGGCAGGCGGGGATCGTTATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGGTCAGCGCATGGCTGa                                                                                                                                                                 >  1:461821/1‑248 (MQ=37)

                                                                           gCCGGATGTGCTTTGCTTTTCCGGACTACAAAATCGCAGCGTGTAGGCTTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCA‑GCGCAGCGCGTCTT‑TAAACGCGGGCAGGCGGGTATCGTTATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGCTGAAGACg                                                                                                                                                            <  2:809136/251‑1 (MQ=255)

                                                                           gCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCA‑GCGCAGCGCGTCTT‑TAAACGCTGGCAGGCGGGTATCGTTATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGCTGAAGACg                                                                                                                                                            <  2:313327/251‑1 (MQ=38)

                                                                             cggatggtcTTTGATTATCCGGCCTACAAAAACGCAGCGGGTAGGCCCGATAAGACGCGGCAGCGTCGCATCAGTCGTTACACATCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCA‑GCGCAGCGCGTCTT‑TAAACGCTGGCAGGCGGGTAGCGTTATCAAGCACCGTGATTTTAATGTCGTGCATCTCGGCGAACTGTCGCATATCGTTGAGGTTCAGCGCATGGCTGAATACGGt                                                                                                                                                          <  2:687803/243‑1 (MQ=255)

                                                                              ggATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCA‑GCGCAGCGCGTCTT‑TAAACGCTGGCAGGCGGGTATCGTTATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGCTGAAGACGGTa                                                                                                                                                         <  1:173395/251‑1 (MQ=38)

                                                                                  gcgcTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCA‑GCGCAGCGCGTCTT‑TAAACGCTGGCAGGCGGGTATCGTTATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGCTGAAGACGGTATgg                                                                                                                                                      <  1:137351/250‑1 (MQ=38)

                                                                                   cgcTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTGCGTTCCA‑GCGCAGCGCGTCTT‑TAAACGCTGGCAGGCGGGTATCGTTATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGCTGAAGACGGTATGGTg                                                                                                                                                    <  1:218024/251‑1 (MQ=38)

                                                                                            tATCCGGCCTACAAAATCTCAGCGTGTAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCA‑GCGCAGCGCGTCTT‑TAAACGCTGGCAGGCGGGTATCGTTATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGCTGAAGACGGTATGGTGCGCGcca                                                                                                                                             <  1:77961/249‑1 (MQ=255)

                                                                                             aTCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGTCAGGGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCA‑GCGCAGCGCGTCTT‑TAAACGCTGGCAGGCGGGTATCGTTATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGCTGAAGACGGTATGGTGCGCGccacca                                                                                                                                          <  2:810264/251‑1 (MQ=255)

                                                                                                  gCCTACAAAATCGCAGAGTGTAGGCCTGATAAGACGCGTAAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCA‑GCGCAGCGCGTCTT‑TAAACGCTGGCAGGCGGGCATCGTTTTCAATCACCGTGATTTCAAATTCGTGCATCTCGGCGAACTGGGGCGTAACGTTGAGGTTCCACGCACGGCTTGAGACGGTATGGTGCGCGccccccgaggg                                                                                                                                     >  2:595543/1‑242 (MQ=255)

                                                                                                   ccTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCA‑GCGCAGCGCGTCTT‑TAAACGCTGGCAGGCGGGTATCGTTATCAATCCCCGTGATTTCAATGTCGTGCATCTCGGCGAACTGGCTCATATCGTTGAGGGTCCGAGCATGGCTGCAGACGGTATGGTGCGCGCCCCCCGCGAgg                                                                                                                                    >  2:347618/1‑251 (MQ=255)

                                                                                                      aCAAAAGCGGAGCGTGTAGGCCTGATAAGCCGCGTCAGCAGAGCAGCAGGCGTTACACAACGGAGGCGACTACGTAGCGACGAAATCCGTAATACACTTCGTGCCA‑GCGCAGCGCGTCTT‑TAAACGCTGGCAGGCGGGTATCGTGATCAATCACCGTGATTTCAATGTCGTGCATCGCGGCGAACTGGCGAATATCGTTGAGGTTCAGCGCAGGGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGA‑Tc                                                                                                                                <  2:319158/251‑1 (MQ=255)

                                                                                                        aaaaACGCAGCGTGTAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCA‑GCGCAGCGCGTCTT‑TAAACGCTGGCAGGCGGGTATCGTTATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGA‑TCCa                                                                                                                              <  2:135556/251‑1 (MQ=255)

                                                                                                         aaaTCGCCGCGTGTAGGCCTGATAAGACGCGACAGCGTCGCAACAGGCGTTACGCACCTGATGCGACTACGTAGCGACGAAATCCGTAATACACTTCGTTCCA‑TCGCAGCGCGTCTT‑TAAACGCTTGCAGGCGGGTATCGGTGTCAATCACCGTGATTGCAAGGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGCTGAAGACGGTATGGTGCGCGCCACCAGCGATGA‑TCCAc                                                                                                                             <  2:205031/251‑1 (MQ=255)

                                                                                                         aaaTCGCAGCGTGTAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGCTGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCA‑GCGCAGCGCGTCTT‑TAAACGCTGGCAGGCGGGGATCGTTTTCAATCACCGAGATTTATATGTCGGGCATCTCTGCGAACTGGCGCCTATCGTTGGGGGTCAGCGCATGGCTTGAGACGGGATGGCGCGCGccccccgcgcgagtacac                                                                                                                              >  2:758493/1‑235 (MQ=255)

                                                                                                          aaTCGCAGCGTGTAGGCCTGATAAGCCGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCA‑GCGCAGCGCGTCTT‑TAAACGCTGGCAGGCGGGGATTGTTATCAATCACCGTGATTTTAATGTCGTGACTCCCGTGGAACTGGCGCATATCGATGGGGGTCAGCGCACGGCTGAAGACGGTATGGGgcgccacccccacgaggagccccc                                                                                                                             >  2:512931/1‑231 (MQ=255)

                                                                                                             cGCAGCGTGTAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACCCTTCGTTCCA‑GCGCATCGCGTCTA‑TAAACGCTGGCAGGCTGGTATCGTTATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGATGTTCAGCGCATGGCTGAAGACGGTTTGGTGCGCGCCACCAGCGAGGA‑TCCACGCtt                                                                                                                         <  1:584342/251‑1 (MQ=255)

                                                                                                              gCAGCGTGTAGGCCTGATAATACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCA‑GCGCAGCGCGTCTT‑TAAACGCTGGCAGGCGGGTATCGTTATCAATCACCGTGATTTCAATGTCGTGCATCCCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGA‑TCCACGCTTc                                                                                                                        <  1:911505/251‑1 (MQ=255)

                                                                                                              gCAGCGTGTAGGCCAGATAAGACGCGTCAGCGTCGCATCAGGCGTTACATACCGGATGCGGCTACTTAGCGACGAAACCCGTAATACACTTCGTTCCA‑GCGCAGCGCGTCTT‑TAAACGCTGGCAGGCGTGTGTCGTTATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAATTGGCGCATATCGTTGAGGTTCAGTGCATGGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGA‑TCCACGCTTc                                                                                                                        <  1:496030/251‑1 (MQ=255)

                                                                                                                aGCGTGTATGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGACATCCGTAATACACTTCGTTCCA‑GCGCAGCGCGTCTT‑TAAACGCTGGCAGGCGGGGATCGTTATAAATAACCGTGATTTCAATGTCGGGCAACCCGGCGAACATGCGCCTATTGTTGAGGTTCAGCGACTGGCCGAAGAAGGACTGGTTCGCGCCACCAGCCAGGacacacgccttcg                                                                                                                       >  2:167525/1‑239 (MQ=255)

                                                                                                                 gCGTGTAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCA‑GCGCAGCGCGTCTT‑TAAACGCTGGCAGGCGGGTATCGTTATCAAACACCGTGATTTCAATGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGA‑TCCACGCTTCgg                                                                                                                      >  1:449839/1‑250 (MQ=255)

                                                                                                                         gcctgATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCA‑GCGCAGCGCGTCTT‑TAAACGCTGGCAGGCGGGTATCGTTATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGCTGAAGACGGTATGGGGCGCGCCACCAGCGAGGA‑TCCACGCTTCGGAA‑GCAGTTg                                                                                                            >  1:504026/5‑251 (MQ=255)

                                                                                                                          cctgATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCA‑GCGCAGCGCGTCTT‑TAAACGCTGGCAGGCGGGTATCGTTATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATAGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGA‑TCCACGCTTCGGAA‑GCAGTTgg                                                                                                           <  1:241907/248‑1 (MQ=255)

                                                                                                                              aTAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCA‑GCGCAGCGCGTCTT‑TAAACGCTGGCAGGCGGGTATCGTTATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGCTGAAGACGGTATGGGGCGCGCCACCAGCGAGGA‑TCCACGCTTCGGAA‑GCAGTTGGc                                                                                                          >  1:702692/1‑248 (MQ=255)

                                                                                                                                      cgTCAGCGTCGCATCAGGCGGTACTCATCTGATTCGTCTTCTTAGCGGCGAAAGCCGTAATTCACTTCGTTCCA‑GCTCATCGCGTCTT‑TAAACGCTGGCAGGCGGGTATCGTTATCAATCACCGTTATTTCAATGTCGTGCATCTCGGCGAACTGGCGCGTATCGTTGAGGTTCAGCTCATGGCTGAAGACGGTTTGGTGCGCGCCACCCGCGAGGA‑TCCACGCTTCGGAA‑GCAGTTGGCAGATCCGGTTg                                                                                               <  2:353220/251‑1 (MQ=255)

                                                                                                                                        tCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCA‑GCGCAGCGCGTCTT‑TAAACGCTGGCAGGCGGGTATCGTTATCAATCACCGTGATTTCCATTTCGTGCCTCTCGGCGGACTGGCGCCTATCGTTGAGGTTCAGCGCATGGCTGAAGGCGGTATGGTGCGGGCCACCAGCGGGGA‑TCCCCCCTTCGGAA‑GCAGTTGGCCGATCCGGTTgtg                                                                                             >  2:520113/1‑249 (MQ=255)

                                                                                                                                          aGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCA‑GCGCAGCGCGTCTT‑TAAACGCTGGCAGGCGGGTATCGTTATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGA‑TCCACGCTTCGGAA‑GCAGTTGGCAGATCCGGTTGCGCt                                                                                           <  1:269609/251‑1 (MQ=255)

                                                                                                                                             gTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGCCGAAATCCGTAATACACTTCGTTCCA‑GCGCAGCGCGTCTT‑TAAACGCTGGCAGGCGGGGATCGTTATCAATCCCCGTGCTTTCAATGTCGTGCATCTCGGGGAACTGGCGCATATCGTTGAGGTTCCGCGCATGGCTGAAGACGGTATGGTTCGCGACACACGCGAGGA‑TCCACGCTTCGGAA‑GAAGTTGGCAGATCCGGTTGCGCtttt                                                                                        >  1:645861/1‑250 (MQ=255)

                                                                                                                                                gCATTAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCA‑GCGCAGCGCGTCTT‑TAAACGCTGGCAGGCGGGTATCGTTATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGA‑TCCACGCTTCGGAA‑GCAGTTGGCAGATCCGGTTGCGCTTTCcac                                                                                     <  1:717794/251‑1 (MQ=255)

                                                                                                                                                 cATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCA‑GCGCAGCGCGTCTT‑TAAACGCTGGCAGGCGGGTATCGTTATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGAATGGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGA‑TCCACGCTTCGGAA‑GCAGTTGGCAGATCCGGTTGCGCTTTCcaca                                                                                    <  1:347618/251‑1 (MQ=255)

                                                                                                                                                  aTCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCA‑GCGCAGCGCGTCTT‑TAAACGCTGGCAGGCGGGTATCGTTTTCAATCACCGCGGTTTCAATGTCGTGCCTCTCGGCGAAATGGCGCCTATCGCTTAGGTTTAGCGCATGGCTGAAGACGGTAAGGGGCGCGCCCCCAGCGAGGA‑TCCACCCTTCGGAA‑GAAGTTGGCAGATCCCGATGCGCTTTcccccc                                                                                   >  2:200542/1‑247 (MQ=255)

                                                                                                                                                       gCGTTACACCCCTGATGCGTCCACTTAGCGACGAAATCCGTAATACACTTCGTTCCA‑GCGCAGCGCGTCTT‑TAAACGCTGGCAGGCGGGTATCGTTATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGA‑TCCACGCTTCGGAA‑GCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCAt                                                                              <  2:519981/251‑1 (MQ=255)

                                                                                                                                                       gCGTTACACACCTGATGCGACTTCTTAGCGACGAAATCCGTAATACACTTCGTTCCA‑GCGCAGCGCGCCTT‑TAAACGCTGGCAGGCGGGTATCGTTATCAATCACCGTGATTTCCATGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGGTCAGCGCATGGCTTAAGACGGTATGGTGCGCGCCACCAGCGAGGA‑TCCACGCTTCGGAA‑GCAGTTTGCAGATCCGGTTGCGCTTTCCACAGCGCAt                                                                              <  2:104429/251‑1 (MQ=255)

                                                                                                                                                        cGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCA‑GCGCAGCGCGTCTT‑TAAACGCTGGCAGGCGGGTATCGTTATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGA‑TCCACGCTTCGGAA‑GCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCAtt                                                                             <  2:167406/251‑1 (MQ=255)

                                                                                                                                                        cGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCA‑GCGCAGCGCGTCTT‑TAAACGCTGGCAGGCGGGTATCGTTATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGAGCATGGCTGAAGACGGTATGGGGCGCGGCACCAGCGAGGA‑TCCACGCTTCGGAA‑GCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCAtt                                                                             >  1:938169/1‑251 (MQ=255)

                                                                                                                                                         gTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCA‑GCGCAGCGCGTCTT‑TAAACGCTGGCAGGCGGGTATCGTTATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAAATGGCGCATATCGTTGAGGTTCAGCGCATGGCTGAAGACGGGATTGGGCGCGCCACCAGCGAGGA‑TCCACGCTTCGGAA‑GCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTc                                                                            >  1:868891/1‑251 (MQ=255)

                                                                                                                                                          ttACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCA‑GCGCAGCGCGTCTT‑TAAACGCTGGCAGGCGGGTTTCGTTATCAATCACCGTGATTTCAATGTCTTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCCACGCCTGGCTGAAGACGGTATGGTGCGCGCCCCCCGCGGGGA‑TCCACGCTTCGGAA‑GCCGTTTGCAGATTCGGTTGCGCCTTCCACAGCGCATTct                                                                           >  1:349276/1‑250 (MQ=255)

                                                                                                                                                            acacacctgATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCA‑GCGCAGCGCGTCTT‑TAAACGCTGGCAGGCGGGTATCGTTATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGA‑TCCACGCTTCGGAA‑GCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGcc                                                                         <  1:921060/243‑1 (MQ=255)

                                                                                                                                                                 cctgATGCGACTACTTCGCGACGAAAGCCGTAATCCACTTCGTTCCA‑GCGCAGCGCGTCTT‑TAAACGCTGGCAGGCGGGTATCGTTATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGA‑TCCACGCTTCGGAA‑GCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCgg                                                                    <  2:632705/248‑1 (MQ=255)

                                                                                                                                                                    gATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCA‑GCGCAGCGCGTCTT‑TAAACGCTGGCAGGCGGGTATCGTTATCAATCACCGTGATTTCAATGTCGTGCACCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGTA‑TCCACGCTTCGGAA‑GCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAg                                                                 <  2:406851/251‑1 (MQ=255)

                                                                                                                                                                      ggcgacttactAGCGACGAAATCCCTAATACACTTTGGTCCAAGC‑CAGGGCGGCTT‑TAAACGCTGGCAGGCGGGTAGCGTTATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGA‑TCCACGCTGCGGAA‑GCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGtt                                                               <  2:954197/241‑1 (MQ=255)

                                                                                                                                                                        cgacTACTTAGCGACGAAATCCGTAATACACTTCGTTCCA‑GCGCAGCGCGTCTT‑TAAACGCTGGCAGGCGGGTATCGTTATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGTGGTTCAGCGCATGGCTGGAGGCGGGATGGTGCGCGCCCCCAGCGAGGA‑TCCACGCCTCGGAA‑GCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCCTTTGCCACCGGCAGtttt                                                             >  2:255382/4‑250 (MQ=255)

                                                                                                                                                                        cgacTACTGAGCGACTAAATCCGTAATACACTTCGTTCCA‑GCGCAGCGCGTCTT‑TAAACGCTGGCAGGCGGGTATCGTTAGCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGA‑TCCACGCTTCGGAA‑GCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTc                                                             <  2:611681/248‑1 (MQ=255)

                                                                                                                                                                         gacTACTTAGCGACGAAATCCGTAATACACTTCGTTCCA‑GCGCAGCGCGTCTT‑TAAACGCTGGCAGGCGGGTCTCGTTATCCATCACCGGGATTTCAATGTCGAGCATCCCGGCGAACTGGCGCATCTCGGTGAGGGTAAGCGCATGGCTGAAGACGGTATGGTGCGCGCCCCCAACGGGGG‑TCCACCCTTCGGAACGGAGT‑GGCAGGTCCGGGTGGGCTTTCCACCGCGCCATCGCCACCAGCaacttcg                                                            >  2:486716/3‑245 (MQ=255)

                                                                                                                                                                         gacTACTTAGCGACGAAATCCGTAATACACTTCGTTCCA‑GCGCAGCGCGTCTT‑TAAACGCTGGCAGGCGGGTATCGTTATCAATCACCCTGATTTCCATGTCTCGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATAGCGGAAGACGGTATGGTGCGCGCCCCCCGCGAGGA‑TCCACGCTTAGGAA‑GCCGTTGGGAGATCCGGTTGCGCCTTCCACCGCGCCTTctccccaggccgagttc                                                            >  2:29398/3‑235 (MQ=255)

                                                                                                                                                                          actacgtaacgtcgaatacctgaaTACACTTCGTTCCA‑GCACAGC‑CGTTTTGTAAGCGCTGGCAGGTGGGTATCGTTATCAATCTCCGTGATTTCAATGGCTAGCATCTCGGCTAACTGGCGCGTATCGTTGACGTGCAGCGCTTGGTTGAAGACGGGAGTGTGCTCGCCACCAGCGAGGA‑TCCACGCTTCGGAA‑GCAGTGGGCATAGCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGTCACTTTCgt                                                           <  2:397312/229‑2 (MQ=255)

                                                                                                                                                                          acTACTTAGCGACGAAATCCGTAATACACTTCGTTCCA‑GCGCAGCGCGTCTT‑TAAACGCTGGCAGGCGGGTATCGTTATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGA‑TCCACGCTTCGGAA‑GCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCgg                                                           <  1:408356/250‑1 (MQ=255)

                                                                                                                                                                          acTACTTAGCGACGAAATCCGTAATACACTTCGTTCCA‑GCGCAGCGCGTCTT‑TAAACGCTGGCAGGCGGGGATCGTTTTCAATCACCGGGGTTTCCATGTCGTGCCTCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGTGCATGGCTGCAGACGGTATGGTGTCCGCCACCGGCGGAGT‑GCCCCGCCTCGGGA‑GGAGTTGGCAGAGCCCGTTGCGCTTTCCACCGCCCATTCTCCCCCGGccgattccc                                                           >  2:274558/2‑243 (MQ=255)

                                                                                                                                                                             aCTTAGCGACGAAATCCGTAATACACTTCGTTCCA‑GCGCAGCGCGTCTT‑TAAACGCTGGCAGGCGGGTATCGTTATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGCTGAAGACGGTATGGTGCGCGCAACCAGCGAGGA‑TCCACGCTTCGGAA‑GCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCa                                                         <  1:668344/250‑1 (MQ=255)

                                                                                                                                                                              cTTAGCGACGAAATCCGTAATACACTTCGTTCCA‑GCGCAGCGCGTCTT‑TAAACGCTGGCAGGCGGGGATCGTTATCAATCACCGTGGTTTCAATGTCGTGCCTCTCGGCGAACTGGCGAATATCGTTGAGGTTCAGAGCGTGGCTGGAGACGGTAGGGGGCGCGCCACCAGCGAGGA‑TCCACGCTTCGGAA‑ACAGTTGGCAGATCCGGTTGCGCATTCCACAGCGCCATCGCCACAGGCCGTTTCGGcgcg                                                       >  1:295358/1‑248 (MQ=255)

                                                                                                                                                                                 aGCGACGAAATCCTGAATACACGTCGTTCCA‑GCGCAGCGCGTCTT‑TAAACGCTTGCAGGCGGGTATCGTTATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGA‑TCCACGCTTCGGAA‑GCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGa                                                     <  2:296435/250‑1 (MQ=255)

                                                                                                                                                                                       gaAATCCGTAATACACTTCGTTCCA‑GCGCAGCGCGTCTT‑TAAACGCTGGCAGGCGGGTATCGTTATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGA‑TCCACGCTTCGGAA‑GCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGgt                                              <  1:420463/251‑1 (MQ=255)

                                                                                                                                                                                        aaatccGTAATACACTTCGTTCCA‑GCGCAGCGCGTCTT‑TAAACGCTGGCAGGCGGGTATCGTTATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGA‑TCCACGCTTCGGAA‑GCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGgtg                                             <  1:708766/247‑1 (MQ=255)

                                                                                                                                                                                         aatccGTAATACACTTCGTTCCA‑GCGCAGCGCGTCTT‑TAAACGCTGGCAGTCTGGTATCGTTATCAATCACCGTGATTTCAATGTCGTGCATCGCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGA‑TCCACGCTTCGGAA‑GCAGTTGGCAGATCCGGTTGCGTTTTCCACAGCGTATTCGCCACCGGCAGTTTCGGCATGTAGTGCGgtgt                                            <  1:204740/248‑1 (MQ=255)

                                                                                                                                                                                          atccGTAATACACTTCGTTCCA‑GCGCAGCGCGTCTT‑TAAACGCTGGCAGGCGGGTATCGTTATCAATCACCGTGGTTTTAATGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGCTGAAGACGGTATGGTGCGCGCCACAAGCGAGGA‑TCCACGCTTCGGAC‑GCAGTTGGAAGATCCGGTTGCGCTTTTCACAGCGCATTCGCCACCGGCAGATTAGGCAGGGAggtgggtggt                                           >  1:361458/3‑242 (MQ=255)

                                                                                                                                                                                           cccGTAATACACTTCGTTCCA‑GCGCAGCGCGTCTT‑TAAACGCTGGCAGGCGGGTATCGTTATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGA‑TCCACGCTTCGGAA‑GCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGttt                                          <  2:495778/251‑1 (MQ=255)

                                                                                                                                                                                                 aTACACTTCGTTCCA‑GCGCAGCGCGTCTT‑TAAACGCTGGCAGGCGGGTATCGTTATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGA‑TCCACGCTTCGGAA‑GCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCg                                    <  1:480321/251‑1 (MQ=255)

                                                                                                                                                                                                  tACACTTCGTTCCA‑GCGCAGCGCGTCTT‑TAAACGCTGGCAGGCGGGTATCGTTATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGCTGAAGACGGTATGGGGCGCGCCACCAGCGAGGA‑TCCACGCTTCGGGA‑GCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCCGTTTCGGCCGGGGGTGCGGTGTTTTCACCgg                                   >  1:332450/1‑250 (MQ=255)

                                                                                                                                                                                                     ccTTCGTTCCA‑GCGCAGCGCGTCTT‑TAAACGCTGTCAGGCGGGTATCGTTATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGA‑TCCACGCTTCGGAA‑GCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTc                                <  2:95149/250‑1 (MQ=255)

                                                                                                                                                                                                     acTTCGTTCCA‑GCGCAGCGCGTCTT‑TAAACGCTGGCAGGCGGGTATCGTTATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGA‑TCCACGCTTCGGAA‑GCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTc                                <  1:412330/251‑1 (MQ=255)

                                                                                                                                                                                                       ttCGTTCCA‑GCGCAGCGCGTCTT‑TAAACGCTGGCAGGCGGGTATCGTTATCAATCACCGGGATTTCAATGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGCTGAAGACGGGATGGGGCGCGCCCACAGCGAGGA‑TCCACGCTTCGGAA‑GCAGTTGGCAGATCCGGTTTCGCTTTCCCCAGCGCATTCGCCACCGGCCGTTTTGGCAGGGGGTGGGGGGTTTTCAcccgggcgg                              >  2:401323/1‑244 (MQ=255)

                                                                                                                                                                                                          gTTCCA‑GCGCAGCGCGTCTT‑TAAACGCGGGCAGGCGGGTATCGTTATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGA‑TCCACGCTTCGGAA‑GCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGAtac                           <  1:662150/251‑3 (MQ=255)

                                                                                                                                                                                                             ccA‑GCGCAGCGCGTCTT‑TAAACGCTGGCAGGCGGGTATCGTTATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGA‑TCCACGCTTCGGAA‑GCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAg                         <  1:691235/250‑1 (MQ=255)

                                                                                                                                                                                                             ccA‑GCGCAGCGCGTCTT‑TAAACGCTGGCAGGCGGGTATCGTTATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGA‑TCCACGCTTCGGAA‑GCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAg                         <  1:981795/250‑1 (MQ=255)

                                                                                                                                                                                                               a‑gcgcagcgcGTCTT‑TAAACGCTGGCAGGCGGGTATCGTTATCAATCCCCGGGCTTTCAATGTCGTGCCTCTCTGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATTGCTGAAGGAGGTTTGGATCGCGCCACCAGCCAGGGGTCC‑CGCTTTGGGA‑GCAGTTGGCCGATCCGGTTGCGCTTTTCACAGCGCATTTCCCACCGGCAGGTTCGGCAGGGAGGGCGGGGTTTTTTCCGTGTCGGTGCactgt                      >  2:963081/1‑247 (MQ=255)

                                                                                                                                                                                                               a‑gcgcagcgcGTCTT‑TAAACGCTGGCAGGCGGGTATCGTTATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGCTGAAGACGGTATGGTGCGCGCCATCAGCGAGGA‑TCCACGCTTCGGAA‑GCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTa                      <  1:551869/251‑1 (MQ=255)

                                                                                                                                                                                                                 gcgcagcgcGTCTT‑TAAACGCTGGCAGGCGGGTATCGTTATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGCTGAAGACGGTATGGTGCGCGCCCCCAGCGAGGA‑TCCACGCTTCGGAA‑GCAGTTGGCAGAGCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTaa                     <  2:504026/251‑1 (MQ=255)

                                                                                                                                                                                                                    cagcgcGTCTT‑TAAACGCTGGCAGGCGGGGATCGTTATCAATCACCGTGATTTCAATGTCGGGCATCTCGGCGAACTGGCGCCTATCGTTGAGGTTCAGCGCATGGCTGAAGACGGTATGGGGCGCGCCACCCGCGAGGG‑TCCACGCTTCGGAA‑GCAGTTGGCAGATCCGGGTGCGCTTTCCCCAGCGCCTTCGCCCCCGGCAGGTTCGGCAGGGGGGGCGGGGTTTTTACCCTGTCGGAACAGTTAAcc                   >  2:339706/1‑250 (MQ=255)

                                                                                                                                                                                                                        gcgTCTT‑TAAACGCTGGCAGGCGGGTATCGTTATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGA‑TCCACGCTTCGGAA‑GCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAgcag              <  1:652328/251‑4 (MQ=255)

                                                                                                                                                                                                                               tgaaaCGCTGGCAGGCGGGTATCGTGATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGA‑TCCACGCTTCGGAA‑GCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACgg          <  1:2236/247‑1 (MQ=255)

                                                                                                                                                                                                                                 aaactcgggCAGGCGGGTATCTTTATCAATCACCTGGGTTTCAATGTCGTGCATCTCGGCGAATTGGCGCATATCGTTGAGGTTCAGCGCATGGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGA‑TCCACGCTTCGGAA‑GCAGTTGGCAGAGCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTaa       <  2:513033/243‑1 (MQ=255)

                                                                                                                                                                                                                                 aaaCGCTGGCAGGCGGGTATCGTTATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGA‑TCCACGCTTCGGAA‑GCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAAc      <  1:381576/251‑1 (MQ=255)

                                                                                                                                                                                                                                 aaaCGCTGGCAGGCGGGGATCGGTATCAATCACCGAGATTTCAATGTCGTGCATCTCGGCGAACTGGCGCAAATCGTTGAGGTTCAGCGCATGGCTGAAGACGGTATGGTGCGCGCCACCAGCGGGGA‑TCCACGCTTCGGAA‑GCAGTTGGCAGATCCGGGTGCGCTTTCCCCAGCGCATTCGCCACCGGCAGGTTCGGCAGGGGGGGGGGGGGTTTTACCGTGTCGATAAAGGTAACCAGCAGAAGGTaa       >  1:389155/1‑250 (MQ=255)

                                                                                                                                                                                                                                    cGCTGGCAGGCGGGTATCGTTATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGA‑TCCACGCTTCGGAA‑GCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGAt   <  1:878655/251‑1 (MQ=255)

                                                                                                                                                                                                                                     gCTGGCAGGTGGGTATCGTTATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGA‑TCCACGCTTCGGAA‑GCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATc  <  2:233832/251‑1 (MQ=255)

                                                                                                                                                                                                                                     gCTGGCAGGCGGGTATCGTTATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCATGGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGA‑TCCACGCTTCGGAA‑GCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATc  <  1:285187/251‑1 (MQ=255)

CTTTGCTTATCCGGCCTACAAAATCGCAGCGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCAGATAAGACGCGTCAGCGTCGCATCAGGCGTTACATACCGGATGCGGCTACTTAGCGACGAAACCCGTAATACACTTCGTTCCA‑GCGCAGCGCGTCTT‑TAAACGCTGGCAGGCGTGTGTCGTTATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAATTGGCGCATATCGTTGAGGTTCAGTGCATGGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGA‑TCCACGCTTCGGAA‑GCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATGCAGTTAACCAGTAGACGGTAACGATC  >  NC_000913/66660‑67137

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`