BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	66,898	T→G	T480T (ACA→ACC)	araA ←	L‑arabinose isomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	66,898	0	T	G	98.5%	398.5 / ‑1.7	129	T480T (ACA→ACC)	araA	L‑arabinose isomerase
Reads supporting (aligned to +/- strand): ref base T (0/2); new base G (48/79); total (48/81)
Fisher's exact test for biased strand distribution p-value = 5.29e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.91e-01

Only 100 of 130 total aligned reads displayed.

GGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCAGATAAGACGCGTCAGCGTCGCATCAGGCGTTACATACCGGATGCGGCTACTTAGCGACGAAACCCGTAATACACTTCGTTCCA‑GCGCAGCGCGTCTT‑TAAACGCTGGCAGGCGTGTGTCGTTATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAATTGGCGCATATCGTTGAGGTTCAGTGCAT‑GGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGA‑TCCACGCTTCGGAA‑GCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATGCAGTTAACCAGTAGACGGTAACGATC  >  NC_000913/66653‑67137

       cTTTGCTTATCCGGCCTACAAAATCGGAACGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCA‑GCGCAGCGCGTCTT‑TAAACGCTGGCAGGCGGGGATCGTTatc                                                                                                                                                                                                                                         >  1:372730/1‑250 (MQ=18)

           gCTTATCCGGCCTACAAAATCGGAACGCGTAGGCCTGATAAGACGCGCCAGCGGCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCA‑GCGCAGCGCGTCTT‑TAAACGCTGGCAGGCGGGTATCGTTatcaatca                                                                                                                                                                                                                                    <  1:177175/251‑1 (MQ=21)

           gCTTAGCCGGCCTACAAAATCGGAACGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCA‑GCGCAGCGCGTCTT‑TAAACGCTGGCAGGCGGGTATCGTTatcaatca                                                                                                                                                                                                                                    <  1:600919/251‑1 (MQ=21)

             ttATCCGGCCTACAAAATCGGAACGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCA‑GCGCAGCGCGTCTT‑TAAACGCTGGCAGGCGGGGAACGTTATCAATCAcc                                                                                                                                                                                                                                  >  1:158268/1‑251 (MQ=18)

              tcaccgtccagcAAAATCGGAACGCGTAGGCCTGCTTAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATTCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTGAGCGACGAAATCCGTAATTCACTTCGTTCCA‑GCGCCGCGCGTCTT‑TAAACGCTGTCAGGCGGGTATCGTTATCAATCAc                                                                                                                                                                                                                                   <  2:67473/238‑1 (MQ=18)

                        aCAAAATCGGAACGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCA‑GCGCAGCGCGTCTT‑TAAACGCTGGCAGGCGGGTATCGTTATCAATCACCGTGATTTCaa                                                                                                                                                                                                                        <  1:575701/250‑1 (MQ=21)

                                   acgcgTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCA‑GCGCAGCGCGTCTT‑TAAACGCTGGCAGGCGGGTATCGTTATCAATCACCGTGATTTCAATGTCGTGCAtct                                                                                                                                                                                                            <  1:738691/250‑1 (MQ=25)

                                     gcgTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCA‑GCGCAGCGCGTCTT‑TAAACGCTGGCAGGCGGGTATCGTTATCAATCACCGTGATTTCAATGTCGTGCATCTCg                                                                                                                                                                                                          <  1:172253/251‑1 (MQ=25)

                                         aGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCA‑GCGCAGCGCGTCTT‑TAAACGCTGGCAGGCGGGTATCGTTATCAATCACCGTGATTTCAATGTCGGGCCTCTCGGCg                                                                                                                                                                                                       >  1:611681/1‑250 (MQ=25)

                                               gtttaGACGAGCTAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCA‑GCGCAGCGCGTCTT‑TAAACGCTGGCAGGCGGGTATCGGTATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAACTGgc                                                                                                                                                                                                <  2:704827/247‑1 (MQ=25)

                                               gATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTACTACACTTCGTTCCA‑GCGCAGCGCGTCTT‑TAAACGCTGGCAGGCGGGTATCGTTATCATTCACCGTGATTTCAATGTCGTGCATCTCGGTGAACTGgc                                                                                                                                                                                                >  1:515350/1‑251 (MQ=25)

                                                aTAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCA‑GCGCAGCGCGTCTT‑TAAACGCTGGCAGGCGGGTATCGTTATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAACTGgcg                                                                                                                                                                                               <  1:47930/251‑1 (MQ=25)

                                                aTAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCA‑GCGCAGCGCGTCTT‑TAAACGCTGGCAGGCGGGTATCGTTATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAACTGgcg                                                                                                                                                                                               <  1:459363/251‑1 (MQ=25)

                                                         gcCAGCGTCGCATCAGGCGTTGAATGCCGGCTGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGAGTAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCA‑GCGCCGCGCGTCTT‑TAAACGCTGGCAGGCGGGGATCGTTATCAATCACCGTACTTTTCATGGCGTGCATATCGGCGGAATGGCGCATCTCGtt                                                                                                                                                                                      >  1:756626/1‑251 (MQ=18)

                                                         gcCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCA‑GCGCAGCGCGTCTT‑TAAACGCTGGCAGGCGGGTATCGTTATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAACTGGCGCATATCGtt                                                                                                                                                                                      <  1:693596/251‑1 (MQ=37)

                                                            ctcttcgcctcctgCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGCCGAAATCCGTAATACACTTCGTTCCA‑GCGCAGCGCGTCTT‑TAAACGCTGGCAGGCGGGTATCGTTTTCCATCCCCGTGATTTCCATGTCGTGCATCTCGGCGAACTGGCGCATATCCTTggg                                                                                                                                                                                   >  1:168354/14‑249 (MQ=25)

                                                             gCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCA‑GCGCAGCGCGTCTT‑TAAACGCTGGCAGGCGGGTATCGTTATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAgg                                                                                                                                                                                  <  1:414524/251‑1 (MQ=37)

                                                              cGTCGCATCTGGCGTTGAAGTCCGGATGCGCTTTGCGTTTCCGGTCTACCAAAGCGCAGCGTGTAGGGCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACATCTGATGCGACTACGTAGCGACGAAATCCGTAATACACTTCGTTCCA‑GCTCAGAGCGTCTT‑TAAACGCTGGCAGGCGGGTAGCGTTATCAATCACCGTGATTTCAATGTCGGGCATCTCGGCGAACTGGCGCATATCGTTGAGGt                                                                                                                                                                                 <  2:208428/251‑1 (MQ=255)

                                                               gTCGCATCAGGCGTTGAATGCCGGATGCGCTTTTCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCA‑GCGCAGCGCGTCTT‑TAAACGCTGGCAGGCGGGGATCGTTATCAATCAACGTGATTTCAATGTCGTGCATCTCGGCGAAATGGCGCCAATCGTTGAGGtt                                                                                                                                                                                >  1:217463/1‑251 (MQ=25)

                                                                   cATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCA‑GCGCAGCGCGTCTT‑TAAACGCTGGCAGGCGGGGATCGGTATAAATCACAGTGGTTTCAATGTAGTGCATCTCGGCGAAATGGGGCATATCGGTGAGGttaagg                                                                                                                                                                            >  1:844684/1‑247 (MQ=21)

                                                                   cATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCA‑GCGCAGCGCGTCTT‑TAAACGCTGGCAGGCGGGGAACGTTATCAATCACCGGGATTTTTATATCGTGCATCTCGGCCGACTGGCGCATATTGTTGTGGttaggc                                                                                                                                                                            >  2:175604/1‑247 (MQ=21)

                                                                      cAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCA‑GCGCAGCGCGTCTT‑TAAACGCTGGCAGGCGGGTATCGTTATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCa                                                                                                                                                                         <  1:929328/251‑1 (MQ=37)

                                                                      cAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCA‑GCGCAGCGCGTCTT‑TAAACGCTGGCAGGCGGGGATCGTTATCAATCCCCGGGATTTCAATGTCGTGGATCTCCGCGAACTGGCGCATATCGTTGAGGTTCAgcgc                                                                                                                                                                          >  1:429794/1‑247 (MQ=25)

                                                                           gTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCA‑GCGCAGCGCGTCTT‑TAAACGCTGGCAGGCGGGGATCGTTATCCATCACCGTGATTTCAATGTCGTACATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAgcgccttgct                                                                                                                                                                    >  1:599797/1‑242 (MQ=37)

                                                                             ttgaggCCGGATGCGCTTTGCGTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGTCAGCGTCGCATCATGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCA‑GCGCAGCGCGTCTT‑TAAACGCTGGCAGGCGGGTATCGTGATCAATCACCGTGATTTCAAGGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCAT‑GGCTg                                                                                                                                                                  <  2:440771/245‑1 (MQ=37)

                                                                               aaTGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGCCGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCA‑GCGCAGCGCGTCTT‑TAAACGCTGGCAGGCGGGGTTAGTTATAAAACACCGGGGTTTCAATGTTGTGTATCTCGGCGCACTGGCGCCTATCCTTTAGGTGACGTGCAT‑GGccgcc                                                                                                                                                                >  2:601392/1‑246 (MQ=16)

                                                                                  gCCGGATGTGCTTTGCTTTTCCGGACTACAAAATCGCAGCGTGTAGGCTTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCA‑GCGCAGCGCGTCTT‑TAAACGCGGGCAGGCGGGTATCGTTATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCAT‑GGCTGAAGACg                                                                                                                                                            <  2:809136/251‑1 (MQ=255)

                                                                                  gCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCA‑GCGCAGCGCGTCTT‑TAAACGCTGGCAGGCGGGTATCGTTATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCAT‑GGCTGAAGACg                                                                                                                                                            <  2:313327/251‑1 (MQ=38)

                                                                                    cggatggtcTTTGATTATCCGGCCTACAAAAACGCAGCGGGTAGGCCCGATAAGACGCGGCAGCGTCGCATCAGTCGTTACACATCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCA‑GCGCAGCGCGTCTT‑TAAACGCTGGCAGGCGGGTAGCGTTATCAAGCACCGTGATTTTAATGTCGTGCATCTCGGCGAACTGTCGCATATCGTTGAGGTTCAGCGCAT‑GGCTGAATACGGt                                                                                                                                                          <  2:687803/243‑1 (MQ=255)

                                                                                     ggATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCA‑GCGCAGCGCGTCTT‑TAAACGCTGGCAGGCGGGTATCGTTATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCAT‑GGCTGAAGACGGTa                                                                                                                                                         <  1:173395/251‑1 (MQ=38)

                                                                                       attagcTTTGGTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTCCTTAGCGACGAAATTCGTAATACACTTCGTTCCA‑GCGCAGCGCGTCTT‑TAAACGCTGGCAGGCGGGTATCGTTATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCAT‑GGCTGAAGACGGTATg                                                                                                                                                       <  2:327825/247‑1 (MQ=37)

                                                                                         gcgcTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCA‑GCGCAGCGCGTCTT‑TAAACGCTGGCAGGCGGGTATCGTTATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCAT‑GGCTGAAGACGGTATgg                                                                                                                                                      <  1:137351/250‑1 (MQ=38)

                                                                                          cgcTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTGCGTTCCA‑GCGCAGCGCGTCTT‑TAAACGCTGGCAGGCGGGTATCGTTATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCAT‑GGCTGAAGACGGTATGGTg                                                                                                                                                    <  1:218024/251‑1 (MQ=38)

                                                                                           gcTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCA‑GCGCAGCGCGTCTT‑TAAACGCTGGCAGGCGGGGATCGTTATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAACTGGGGCATATCGTTGAGGTTCAGCGCAT‑GGCCGAAGACggacttgtgg                                                                                                                                                   >  1:863966/1‑243 (MQ=37)

                                                                                           gcTTGGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCA‑GCGCAGCGCGTCTT‑TAAACGCTGGCAGGCGGGTATCGTTATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCAT‑GGCTGAAGACGGTATGGTgc                                                                                                                                                   <  2:372730/251‑1 (MQ=38)

                                                                                            cTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCA‑GCGCAGCGCGTCTT‑TAAACGCTGGCAGGCGGGGATCGGTATCAATCACCGTGGTTTCCATGTCGTGCATCTCGGCGAACTGGCGCGTATCGTTGTGGTTCAGCGCAT‑GGCTGAAGACGGTATTGTgcg                                                                                                                                                  >  1:24781/1‑251 (MQ=37)

                                                                                                    aTCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGTCAGGGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCA‑GCGCAGCGCGTCTT‑TAAACGCTGGCAGGCGGGTATCGTTATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCAT‑GGCTGAAGACGGTATGGTGCGCGccacca                                                                                                                                          <  2:810264/251‑1 (MQ=255)

                                                                                                        ggCCTAAAAAATCGTAGCGTGTAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTGACACACCTGATGCGACTACTCAGCGACGAATTCCGTAATACACTTCGTTCCA‑GCGCATCGCGTCTT‑TAAACGCTGGCAGGCGGGTAGCGTTATCAATCACCGGGATTTCAATGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCAT‑GGCTGAAGACGGTATGGTGCGCGCCACCAGCGa                                                                                                                                      <  2:285197/251‑1 (MQ=255)

                                                                                                         gCCTACAAAATCGCAGAGTGTAGGCCTGATAAGACGCGTAAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCA‑GCGCAGCGCGTCTT‑TAAACGCTGGCAGGCGGGCATCGTTTTCAATCACCGTGATTTCAAATTCGTGCATCTCGGCGAACTGGGGCGTAACGTTGAGGTTCCACGCAC‑GGCTTGAGACGGTATGGTGCGCGccccccgaggg                                                                                                                                     >  2:595543/1‑242 (MQ=255)

                                                                                                             aCAAAAGCGGAGCGTGTAGGCCTGATAAGCCGCGTCAGCAGAGCAGCAGGCGTTACACAACGGAGGCGACTACGTAGCGACGAAATCCGTAATACACTTCGTGCCA‑GCGCAGCGCGTCTT‑TAAACGCTGGCAGGCGGGTATCGTGATCAATCACCGTGATTTCAATGTCGTGCATCGCGGCGAACTGGCGAATATCGTTGAGGTTCAGCGCAG‑GGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGA‑Tc                                                                                                                                <  2:319158/251‑1 (MQ=255)

                                                                                                               aaaaACGCAGCGTGTAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCA‑GCGCAGCGCGTCTT‑TAAACGCTGGCAGGCGGGTATCGTTATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCAT‑GGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGA‑TCCa                                                                                                                              <  2:135556/251‑1 (MQ=255)

                                                                                                                aaaTCGCAGCGTGTAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGCTGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCA‑GCGCAGCGCGTCTT‑TAAACGCTGGCAGGCGGGGATCGTTTTCAATCACCGAGATTTATATGTCGGGCATCTCTGCGAACTGGCGCCTATCGTTGGGGGTCAGCGCAT‑GGCTTGAGACGGGATGGCGCGCGccccccgcgcgagtacac                                                                                                                              >  2:758493/1‑235 (MQ=255)

                                                                                                                      cAGCGTGTAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCA‑GCGCAGCGCGTCTT‑TAAACGCTGGCAGGCGGGTATCGTTATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCAT‑GGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGA‑TCCACGCTTCg                                                                                                                       <  1:6759/251‑1 (MQ=255)

                                                                                                                       aGCGTGTATGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGACATCCGTAATACACTTCGTTCCA‑GCGCAGCGCGTCTT‑TAAACGCTGGCAGGCGGGGATCGTTATAAATAACCGTGATTTCAATGTCGGGCAACCCGGCGAACATGCGCCTATTGTTGAGGTTCAGCGACT‑GGCCGAAGAAGGACTGGTTCGCGCCACCAGCCAGGacacacgccttcg                                                                                                                       >  2:167525/1‑239 (MQ=255)

                                                                                                                       aGCGTGTAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGCTGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCA‑GCGCAGCGCGTCTT‑TAAACGCTGGCAGACGGGTCTCGTTATCAATCACCGTGATTTACATGTCGTGCAACTCGGCGAACTGGAGCATGACGTTGATGGTCAG‑GCCTAGGCTGAAGAATGTATGGGTCGAGCCACACGCGAGGA‑TCCACGccttgg                                                                                                                      >  2:213804/1‑246 (MQ=255)

                                                                                                                        gCGTGTAGGCCTGATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCA‑GCGCAGCGCGTCTT‑TAAACGCTGGCAGGCGGGTATCGTTATCAAACACCGTGATTTCAATGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCAT‑GGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGA‑TCCACGCTTCgg                                                                                                                      >  1:449839/1‑250 (MQ=255)

                                                                                                                                gcctgATAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCA‑GCGCAGCGCGTCTT‑TAAACGCTGGCAGGCGGGTATCGTTATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCAT‑GGCTGAAGACGGTATGGGGCGCGCCACCAGCGAGGA‑TCCACGCTTCGGAA‑GCAGTTg                                                                                                            >  1:504026/5‑251 (MQ=255)

                                                                                                                                     aTAAGACGCGTCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCA‑GCGCAGCGCGTCTT‑TAAACGCTGGCAGGCGGGTATCGTTATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGCACTGGCGCATATCGTTGAGGTTAAGCGCAT‑GGCTGAAGACGGTATGGTGCGCGCCACCCGCGAGGA‑TCCACGCTTCGGAA‑GCAGTTGGc                                                                                                          >  1:629216/1‑248 (MQ=255)

                                                                                                                                             cgTCAGCGTCGCATCAGGCGGTACTCATCTGATTCGTCTTCTTAGCGGCGAAAGCCGTAATTCACTTCGTTCCA‑GCTCATCGCGTCTT‑TAAACGCTGGCAGGCGGGTATCGTTATCAATCACCGTTATTTCAATGTCGTGCATCTCGGCGAACTGGCGCGTATCGTTGAGGTTCAGCTCAT‑GGCTGAAGACGGTTTGGTGCGCGCCACCCGCGAGGA‑TCCACGCTTCGGAA‑GCAGTTGGCAGATCCGGTTg                                                                                               <  2:353220/251‑1 (MQ=255)

                                                                                                                                               tCAGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCA‑GCGCAGCGCGTCTT‑TAAACGCTGGCAGGCGGGTATCGTTATCAATCACCGTGATTTCCATTTCGTGCCTCTCGGCGGACTGGCGCCTATCGTTGAGGTTCAGCGCAT‑GGCTGAAGGCGGTATGGTGCGGGCCACCAGCGGGGA‑TCCCCCCTTCGGAA‑GCAGTTGGCCGATCCGGTTgtg                                                                                             >  2:520113/1‑249 (MQ=255)

                                                                                                                                                 aGCGTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCA‑GCGCAGCGCGTCTT‑TAAACGCTGGCAGGCGGGTATCGTTATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCAT‑GGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGA‑TCCACGCTTCGGAA‑GCAGTTGGCAGATCCGGTTGCGCt                                                                                           <  1:269609/251‑1 (MQ=255)

                                                                                                                                                    gTCGCATCAGGCGTTACACACCTGATGCGACTACTTAGCGCCGAAATCCGTAATACACTTCGTTCCA‑GCGCAGCGCGTCTT‑TAAACGCTGGCAGGCGGGGATCGTTATCAATCCCCGTGCTTTCAATGTCGTGCATCTCGGGGAACTGGCGCATATCGTTGAGGTTCCGCGCAT‑GGCTGAAGACGGTATGGTTCGCGACACACGCGAGGA‑TCCACGCTTCGGAA‑GAAGTTGGCAGATCCGGTTGCGCtttt                                                                                        >  1:645861/1‑250 (MQ=255)

                                                                                                                                                       gCATTAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCA‑GCGCAGCGCGTCTT‑TAAACGCTGGCAGGCGGGTATCGTTATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCAT‑GGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGA‑TCCACGCTTCGGAA‑GCAGTTGGCAGATCCGGTTGCGCTTTCcac                                                                                     <  1:717794/251‑1 (MQ=255)

                                                                                                                                                       gCATCAGGCGTTACACACCTGAGGCGATTAATTAGCGACGAAATCCGTAATACCCTTCGTTCCC‑GCGCAGCGAGGCTT‑TAAACGCTGGCAGGCGGGTATCGTTATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCAT‑GGCTGAAGACGGTATGGTGCGCGCCTCCAGCGAGGA‑TCCACGCTTCGGAA‑GCAGTTGTCAGATCCGGTTGCGCTTTCcac                                                                                     <  2:108475/251‑1 (MQ=255)

                                                                                                                                                        cATCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCA‑GCGCAGCGCGTCTT‑TAAACGCTGGCAGGCGGGTATCGTTATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGAAT‑GGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGA‑TCCACGCTTCGGAA‑GCAGTTGGCAGATCCGGTTGCGCTTTCcaca                                                                                    <  1:347618/251‑1 (MQ=255)

                                                                                                                                                         aTCAGGCGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCA‑GCGCAGCGCGTCTT‑TAAACGCTGGCAGGCGGGTATCGTTTTCAATCACCGCGGTTTCAATGTCGTGCCTCTCGGCGAAATGGCGCCTATCGCTTAGGTTTAGCGCAT‑GGCTGAAGACGGTAAGGGGCGCGCCCCCAGCGAGGA‑TCCACCCTTCGGAA‑GAAGTTGGCAGATCCCGATGCGCTTTcccccc                                                                                   >  2:200542/1‑247 (MQ=255)

                                                                                                                                                              gCGTTACACCCCTGATGCGTCCACTTAGCGACGAAATCCGTAATACACTTCGTTCCA‑GCGCAGCGCGTCTT‑TAAACGCTGGCAGGCGGGTATCGTTATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCAT‑GGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGA‑TCCACGCTTCGGAA‑GCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCAt                                                                              <  2:519981/251‑1 (MQ=255)

                                                                                                                                                              gCGTTACACACCTGATGCGACTTCTTAGCGACGAAATCCGTAATACACTTCGTTCCA‑GCGCAGCGCGCCTT‑TAAACGCTGGCAGGCGGGTATCGTTATCAATCACCGTGATTTCCATGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGGTCAGCGCAT‑GGCTTAAGACGGTATGGTGCGCGCCACCAGCGAGGA‑TCCACGCTTCGGAA‑GCAGTTTGCAGATCCGGTTGCGCTTTCCACAGCGCAt                                                                              <  2:104429/251‑1 (MQ=255)

                                                                                                                                                               cGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCA‑GCGCAGCGCGTCTT‑TAAACGCTGGCAGGCGGGTATCGTTATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCAT‑GGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGA‑TCCACGCTTCGGAA‑GCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCAtt                                                                             <  2:167406/251‑1 (MQ=255)

                                                                                                                                                               cGTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCA‑GCGCAGCGCGTCTT‑TAAACGCTGGCAGGCGGGTATCGTTATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGAGCAT‑GGCTGAAGACGGTATGGGGCGCGGCACCAGCGAGGA‑TCCACGCTTCGGAA‑GCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCAtt                                                                             >  1:938169/1‑251 (MQ=255)

                                                                                                                                                                gTTACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCA‑GCGCAGCGCGTCTT‑TAAACGCTGGCAGGCGGGTATCGTTATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAAATGGCGCATATCGTTGAGGTTCAGCGCAT‑GGCTGAAGACGGGATTGGGCGCGCCACCAGCGAGGA‑TCCACGCTTCGGAA‑GCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTc                                                                            >  1:868891/1‑251 (MQ=255)

                                                                                                                                                                 ttACACACCTGATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCA‑GCGCAGCGCGTCTT‑TAAACGCTGGCAGGCGGGTTTCGTTATCAATCACCGTGATTTCAATGTCTTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCCACGCCT‑GGCTGAAGACGGTATGGTGCGCGCCCCCCGCGGGGA‑TCCACGCTTCGGAA‑GCCGTTTGCAGATTCGGTTGCGCCTTCCACAGCGCATTct                                                                           >  1:349276/1‑250 (MQ=255)

                                                                                                                                                                   acacacctgATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCA‑GCGCAGCGCGTCTT‑TAAACGCTGGCAGGCGGGTATCGTTATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCAT‑GGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGA‑TCCACGCTTCGGAA‑GCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGcc                                                                         <  1:921060/243‑1 (MQ=255)

                                                                                                                                                                        cctgATGCGACTACTTCGCGACGAAAGCCGTAATCCACTTCGTTCCA‑GCGCAGCGCGTCTT‑TAAACGCTGGCAGGCGGGTATCGTTATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCAT‑GGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGA‑TCCACGCTTCGGAA‑GCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCgg                                                                    <  2:632705/248‑1 (MQ=255)

                                                                                                                                                                           gATGCGACTACTTAGCGACGAAATCCGTAATACACTTCGTTCCA‑GCGCAGCGCGTCTT‑TAAACGCTGGCAGGCGGGTATCGTTATCAATCACCGTGATTTCAATGTCGTGCACCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCAT‑GGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGTA‑TCCACGCTTCGGAA‑GCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAg                                                                 <  2:406851/251‑1 (MQ=255)

                                                                                                                                                                             ggcgacttactAGCGACGAAATCCCTAATACACTTTGGTCCAAGC‑CAGGGCGGCTT‑TAAACGCTGGCAGGCGGGTAGCGTTATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCAT‑GGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGA‑TCCACGCTGCGGAA‑GCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGtt                                                               <  2:954197/241‑1 (MQ=255)

                                                                                                                                                                               cgacTACTTAGCGACGAAATCCGTAATACACTTCGTTCCA‑GCGCAGCGCGTCTT‑TAAACGCTGGCAGGCGGGTATCGTTATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCAT‑GGCTGTAGACGGTATGGGGCGCGCCACCAGCGAGGA‑TCCACGCTTCGGAA‑GCAGTTGGGAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAggttt                                                             >  1:704961/4‑247 (MQ=255)

                                                                                                                                                                               cgacTACTGAGCGACTAAATCCGTAATACACTTCGTTCCA‑GCGCAGCGCGTCTT‑TAAACGCTGGCAGGCGGGTATCGTTAGCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCAT‑GGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGA‑TCCACGCTTCGGAA‑GCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTc                                                             <  2:611681/248‑1 (MQ=255)

                                                                                                                                                                                gacTACTTAGCGACGAAATCCGTAATACACTTCGTTCCA‑GCGCAGCGCGTCTT‑TAAACGCTGGCAGGCGGGTCTCGTTATCCATCACCGGGATTTCAATGTCGAGCATCCCGGCGAACTGGCGCATCTCGGTGAGGGTAAGCGCAT‑GGCTGAAGACGGTATGGTGCGCGCCCCCAACGGGGG‑TCCACCCTTCGGAACGGAGT‑GGCAGGTCCGGGTGGGCTTTCCACCGCGCCATCGCCACCAGCaacttcg                                                            >  2:486716/3‑245 (MQ=255)

                                                                                                                                                                                gacTACTTAGCGACGAAATCCGTAATACACTTCGTTCCA‑GCGCAGCGCGTCTT‑TAAACGCTGGCAGGCGGGTATCGTTATCAATCACCCTGATTTCCATGTCTCGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCAT‑AGCGGAAGACGGTATGGTGCGCGCCCCCCGCGAGGA‑TCCACGCTTAGGAA‑GCCGTTGGGAGATCCGGTTGCGCCTTCCACCGCGCCTTctccccaggccgagttc                                                            >  2:29398/3‑235 (MQ=255)

                                                                                                                                                                                 actacgtaacgtcgaatacctgaaTACACTTCGTTCCA‑GCACAGC‑CGTTTTGTAAGCGCTGGCAGGTGGGTATCGTTATCAATCTCCGTGATTTCAATGGCTAGCATCTCGGCTAACTGGCGCGTATCGTTGACGTGCAGCGCTT‑GGTTGAAGACGGGAGTGTGCTCGCCACCAGCGAGGA‑TCCACGCTTCGGAA‑GCAGTGGGCATAGCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGTCACTTTCgt                                                           <  2:397312/229‑2 (MQ=255)

                                                                                                                                                                                 acTACTTAGCGACGAAATCCGTAATACACTTCGTTCCA‑GCGCAGCGCGTCTT‑TAAACGCTGGCAGGCGGGTATCGTTATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCAT‑GGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGA‑TCCACGCTTCGGAA‑GCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCgg                                                           <  1:408356/250‑1 (MQ=255)

                                                                                                                                                                                 acTACTTAGCGACGAAATCCGTAATACACTTCGTTCCA‑GCGCAGCGCGTCTT‑TAAACGCTGGCAGGCGGGGATCGTTTTCAATCACCGGGGTTTCCATGTCGTGCCTCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGTGCAT‑GGCTGCAGACGGTATGGTGTCCGCCACCGGCGGAGT‑GCCCCGCCTCGGGA‑GGAGTTGGCAGAGCCCGTTGCGCTTTCCACCGCCCATTCTCCCCCGGccgattccc                                                           >  2:274558/2‑243 (MQ=255)

                                                                                                                                                                                    aCTTAGCGACGAAATCCGTAATACACTTCGTTCCA‑GCGCAGCGCGTCTT‑TAAACGCTGGCAGGCGGGTATCGTTATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCAT‑GGCTGAAGACGGTATGGTGCGCGCAACCAGCGAGGA‑TCCACGCTTCGGAA‑GCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCa                                                         <  1:668344/250‑1 (MQ=255)

                                                                                                                                                                                     cTTAGCGACGAAATCCGTAATACACTTCGTTCCA‑GCGCAGCGCGTCTT‑TAAACGCTGGCAGGCGGGGATCGTTATCAATCACCGTGGTTTCAATGTCGTGCCTCTCGGCGAACTGGCGAATATCGTTGAGGTTCAGAGCGT‑GGCTGGAGACGGTAGGGGGCGCGCCACCAGCGAGGA‑TCCACGCTTCGGAA‑ACAGTTGGCAGATCCGGTTGCGCATTCCACAGCGCCATCGCCACAGGCCGTTTCGGcgcg                                                       >  1:295358/1‑248 (MQ=255)

                                                                                                                                                                                      ttAGCGACGAAATCCGTAATACACTTCGTTCCA‑GCGCAGCGCGTCTT‑TAAACGATGGCAGGCGGGGATCGCTAACAATCACCGCGACTTCAATGACCAGCATCTCGGAGAACCGGCGAATCAAGTTGAGGTTCAGCACAT‑GGATGAAGACGGTAAGGTGCGGGCCACCAGCGAGGA‑TCCACGATTCGGAA‑GGCGTTGGCAGAACCGGTTCCGGTTTCCACAGCGCATTCGCCCACGGGAGTTTCGGCaagg                                                      >  1:467742/1‑248 (MQ=255)

                                                                                                                                                                                              gaAACCCGTAATACACTTCGTTCCA‑GCGCAGCGCGTCTT‑TAAACGCTGGCAGGCGTGTGTCGTTATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAATTGGCGCATATCGTTGAGGTTCAGTGCAT‑GGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGA‑TCCACGCTTCGGAA‑GCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGgt                                              <  1:36424/251‑1 (MQ=255)

                                                                                                                                                                                               aaatccGTAATACACTTCGTTCCA‑GCGCAGCGCGTCTT‑TAAACGCTGGCAGGCGGGTATCGTTATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCAT‑GGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGA‑TCCACGCTTCGGAA‑GCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGgtg                                             <  1:708766/247‑1 (MQ=255)

                                                                                                                                                                                                aatccGTAATACACTTCGTTCCA‑GCGCAGCGCGTCTT‑TAAACGCTGGCAGTCTGGTATCGTTATCAATCACCGTGATTTCAATGTCGTGCATCGCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCAT‑GGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGA‑TCCACGCTTCGGAA‑GCAGTTGGCAGATCCGGTTGCGTTTTCCACAGCGTATTCGCCACCGGCAGTTTCGGCATGTAGTGCGgtgt                                            <  1:204740/248‑1 (MQ=255)

                                                                                                                                                                                                aatccGTAATACACTTCGTTCCA‑GCGCAGCGCGTCTT‑TAAACGCTGGCAGGCGGGTATCGTTATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCAT‑GGCTGAAGACGGTATGGTGCGCGCCACCCGCGAGGA‑TCCACGCTTCGGAA‑GCCGTTGGCAGATCCGGTTGCGCTTTCCCCAGCGCCTTCGCCACCGGCAGTTTCGGCAGGGAGTGCggggt                                            >  1:391553/4‑248 (MQ=255)

                                                                                                                                                                                                  cccGTAATACACTTCGTTCCA‑GCGCAGCGCGTCTT‑TAAACGCTGGCAGGCGGGTATCGTTATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCAT‑GGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGA‑TCCACGCTTCGGAA‑GCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGttt                                          <  2:495778/251‑1 (MQ=255)

                                                                                                                                                                                                        aTACACTTCGTTCCA‑GCGCAGCGCGTCTT‑TAAACGCTGGCAGGCGGGTATCGTTATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCAT‑GGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGA‑TCCACGCTTCGGAA‑GCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCg                                    <  1:480321/251‑1 (MQ=255)

                                                                                                                                                                                                         tACACTTCGTTCCA‑GCGCAGCGCGTCTT‑TAAACGCTGGCAGGCGGGTATCGTTATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCAT‑GGCTGAAGACGGTATGGGGCGCGCCACCAGCGAGGA‑TCCACGCTTCGGGA‑GCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCCGTTTCGGCCGGGGGTGCGGTGTTTTCACCgg                                   >  1:332450/1‑250 (MQ=255)

                                                                                                                                                                                                            ccTTCGTTCCA‑GCGCAGCGCGTCTT‑TAAACGCTGTCAGGCGGGTATCGTTATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCAT‑GGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGA‑TCCACGCTTCGGAA‑GCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTc                                <  2:95149/250‑1 (MQ=255)

                                                                                                                                                                                                            acTTCGTTCCA‑GCGCAGCGCGTCTT‑TAAACGCTGGCAGGCGGGTATCGTTATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCAT‑GGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGA‑TCCACGCTTCGGAA‑GCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTc                                <  1:412330/251‑1 (MQ=255)

                                                                                                                                                                                                                    ccA‑GCGCAGCGCGTCTT‑TAAACGCTGGCAGGCGGGTATCGTTATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCAT‑GGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGA‑TCCACGCTTCGGAA‑GCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAg                         <  1:691235/250‑1 (MQ=255)

                                                                                                                                                                                                                      a‑gcgcagcgcGTCTT‑TAAACGCTGGCAGGCGGGTATCGTTATCAATCCCCGGGCTTTCAATGTCGTGCCTCTCTGCGAACTGGCGCATATCGTTGAGGTTCAGCGCAT‑TGCTGAAGGAGGTTTGGATCGCGCCACCAGCCAGGGGTCC‑CGCTTTGGGA‑GCAGTTGGCCGATCCGGTTGCGCTTTTCACAGCGCATTTCCCACCGGCAGGTTCGGCAGGGAGGGCGGGGTTTTTTCCGTGTCGGTGCactgt                      >  2:963081/1‑247 (MQ=255)

                                                                                                                                                                                                                      a‑gcgcagcgcGTCTT‑TAAACGCTGGCAGGCGGGTATCGTTATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCAT‑GGCTGAAGACGGTATGGTGCGCGCCATCAGCGAGGA‑TCCACGCTTCGGAA‑GCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTa                      <  1:551869/251‑1 (MQ=255)

                                                                                                                                                                                                                        gcgcagcgcGTCTT‑TAAACGCTGGCAGGCGGGTATCGTTATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCAT‑GGCTGAAGACGGTATGGTGCGCGCCCCCAGCGAGGA‑TCCACGCTTCGGAA‑GCAGTTGGCAGAGCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTaa                     <  2:504026/251‑1 (MQ=255)

                                                                                                                                                                                                                           cagcgcGTCTT‑TAAACGCTGGCAGGCGGGTATCGTTATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCAT‑GGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGA‑TCCACGCTTCGGAA‑GCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCa                  <  2:454029/251‑1 (MQ=255)

                                                                                                                                                                                                                           cagcgcGTCTT‑TAAACGCTGGCAGGCGGGTATCGTTATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCAT‑GGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGA‑TCCACGCTTCGGAA‑GCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCa                  <  1:741446/251‑1 (MQ=255)

                                                                                                                                                                                                                           cagcgcGTCTT‑TAAACGCTGGCAGGCGGGGATCGTTATCAATCACCGTGATTTCAATGTCGGGCATCTCGGCGAACTGGCGCCTATCGTTGAGGTTCAGCGCAT‑GGCTGAAGACGGTATGGGGCGCGCCACCCGCGAGGG‑TCCACGCTTCGGAA‑GCAGTTGGCAGATCCGGGTGCGCTTTCCCCAGCGCCTTCGCCCCCGGCAGGTTCGGCAGGGGGGGCGGGGTTTTTACCCTGTCGGAACAGTTAAcc                   >  2:339706/1‑250 (MQ=255)

                                                                                                                                                                                                                               gcgTCTT‑TAAACGCTGGCAGGCGGGTATCGTTATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCAT‑GGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGA‑TCCACGCTTCGGAA‑GCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAgcag              <  1:652328/251‑4 (MQ=255)

                                                                                                                                                                                                                                      tgaaaCGCTGGCAGGCGGGTATCGTGATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCAT‑GGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGA‑TCCACGCTTCGGAA‑GCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACgg          <  1:2236/247‑1 (MQ=255)

                                                                                                                                                                                                                                        aaactcgggCAGGCGGGTATCTTTATCAATCACCTGGGTTTCAATGTCGTGCATCTCGGCGAATTGGCGCATATCGTTGAGGTTCAGCGCAT‑GGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGA‑TCCACGCTTCGGAA‑GCAGTTGGCAGAGCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTaa       <  2:513033/243‑1 (MQ=255)

                                                                                                                                                                                                                                        aaaCGCTGGCAGGCGGGTATCGTTATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCAT‑GGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGA‑TCCACGCTTCGGAA‑GCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAAc      <  1:381576/251‑1 (MQ=255)

                                                                                                                                                                                                                                           cGCTGGCAGGCGGGTATCGTTATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCAT‑GGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGA‑TCCACGCTTCGGAA‑GCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGAt   <  1:878655/251‑1 (MQ=255)

                                                                                                                                                                                                                                            gCTGGCAGGTGGGTATCGTTATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCAT‑GGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGA‑TCCACGCTTCGGAA‑GCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATc  <  2:233832/251‑1 (MQ=255)

                                                                                                                                                                                                                                            gCTGGCAGGCGGGTATCTGTAGCAATCACCGGGATTTCAATGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCAT‑GGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGA‑TCCACGCTTCGGAA‑GCAGTGGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATc  <  2:511647/251‑1 (MQ=255)

                                                                                                                                                                                                                                            gCTGGCAGGCGGGTATCGTTATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAACTGGCGCATATCGTTGAGGTTCAGCGCAT‑GGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGA‑TCCACGCTTCGGAA‑GCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATc  <  1:285187/251‑1 (MQ=255)

GGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCAGATAAGACGCGTCAGCGTCGCATCAGGCGTTACATACCGGATGCGGCTACTTAGCGACGAAACCCGTAATACACTTCGTTCCA‑GCGCAGCGCGTCTT‑TAAACGCTGGCAGGCGTGTGTCGTTATCAATCACCGTGATTTCAATGTCGTGCATCTCGGCGAATTGGCGCATATCGTTGAGGTTCAGTGCAT‑GGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGA‑TCCACGCTTCGGAA‑GCAGTTGGCAGATCCGGTTGCGCTTTCCACAGCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATGCAGTTAACCAGTAGACGGTAACGATC  >  NC_000913/66653‑67137

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`