BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,396,373	C→T	T103T (ACC→ACT)	amiB →	N‑acetylmuramoyl‑L‑alanine amidase B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,396,373	0	C	T	98.2%	327.4 / NA	114	T103T (ACC→ACT)	amiB	N‑acetylmuramoyl‑L‑alanine amidase B
Reads supporting (aligned to +/- strand): ref base C (0/1); major base T (66/45); minor base G (0/2); total (66/48)
Fisher's exact test for biased strand distribution p-value = 1.71e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 114 total aligned reads displayed.

CCGCGACGCTCTCTGATATTCAGGTTTCTAACGGTAATCAACAGGCGCGGATAACGTTGAGTTTTATTGGCGATCCTGATTATGCGTTTAGCCATCAAAGCAAACGCACCGTGGCGCTCGATATCAAACAAACGGGCGTGATTCAGGGACTGCCGTTGTTGTT‑CAGCGGCAATAATCTGGTGAAGGCGATTCGCTCTGGAACGCCTAAAGATGCACAAACGCTACGG‑CTGGTGGTCGATCTTACCGAAAACGGTAAAACCGAAGCGGTGAAGCGGCAGAATGGCAGCAATTACACTGTCGTCT‑TTACGATTAACGCCGA‑TGTGCCGCCACCGCCTCCTCCGCCGCCCGTGG‑TTGCGAAACGCGTTGAAACGC‑CTGCGGTTGTCGCACCGCGCGTCAGCGAACCGGCGCGCAATCCGTTTAAAACGGAAAGTAACCGCACTACGGGTGTTATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGCG  >  NC_000913/4396129‑4396616

ccGCGACGCACTCTGATATTCAGGTTTCTAACGGTAATCAACAGGAGCGGATCACGTTGAGTTTTATTGGCGATCCTGATTATGCGTTTAGCCATCAAAGCAAACGCACCGTGGCGCTCGCTATCAAACAAACGGGCGGGATTCAGGGACTGCCGTTGTTGTT‑CAGCGGCAATAATCTGGTGAAGGCGATTCGCTCTGGAACGCCTAAAGATGCACAAACGCTACTG‑CTGGTGGTCGATCTTACTGAAAAc                                                                                                                                                                                                                                                   >  2:438718/1‑251 (MQ=255)

  gcgACGCTCTCTGATATTCAGGTTTCTAACGGTAATCAACAGGCGCGGATAACGTTGAGTTTTATTGGCGATCCTGATTATGCGTTTAGCCATCAAAGCAAACGCACCGTGGCGCTCGATATCAAACAAACGGGCGTGATTCAGGGACTGCCGTTGTTGTT‑CAGCGGCAATAATCTGGTGAAGGCGATTCGCTCTGGAACGCCTAAAGATGCACAAACGCTACGG‑CTGGTGGTCGATCTTACTGAAAACgg                                                                                                                                                                                                                                                 <  1:586577/251‑1 (MQ=255)

   cgACGCTCTCTGATATTCAGGTTTCTAACGGTAATCAACAGGCGCGGATAACGTTGAGTTTTATTGGCGATCCTGATTATGCGTTTAGCCATCAAAGCAAACGCACCGTGGCGCTCGATATCAAACAAACGGGCGTGATTCAGGGACTGCCGTTGTTGTT‑CAGCGGCAATAATCTGGTGAAGGCGATTCGCTCTGGAACGCCTAAAGATGCACAAACGCTACGG‑CTGGTGGTCGATCTTACTGAAAACGGt                                                                                                                                                                                                                                                >  1:369927/1‑251 (MQ=255)

              gATATTCAGGTTTCTAACGGTAATCAACAGGCGCGGATAACGTTGCGTTTTCTTGGCGATCCTGATTATGCGTTTAGCCATCAAAGCAAACGCCCCGTGGCGCTCGCTATCAACCAAACGGGCGTGATTCAGGGACTGCCGTTGTTGTT‑CAGCGGCAATCATCTGGTGAAGGCGATTCGATTAGGACCGCCTAAAGATGCCCAAACGATACGG‑CTGGTGGTCGTTTTTACTGACAACGTTAAAACCGag                                                                                                                                                                                                                                       >  2:916948/1‑248 (MQ=255)

              gATATTCAGGTTTCTAACGGTAATCAACAGGCGCGGATAACGTTGAGTTTTATTGGCGATCCTGATTATGCGTTTAGCCATCAAAGCAAACGCACCGTGGCGCTCGATATCAAACAAACGGGCGTGATTCAGGGACTGCCGTTGTTGTT‑CAGCGGCAATAATCTGGTGAAGGCGATTCGCTCTGGAACGCCTAAAGATGCACAAACGCTACGG‑CTGGTGGTCGATCTTACTGAAAACGGTAAAACCGAAGc                                                                                                                                                                                                                                     <  1:984772/251‑1 (MQ=255)

                         ttCTAACGGTAATCAACAGGCGCGGATAACGTTGAGTTTTATTGGCGATCCTGATTATGCGTTTAGCCATCAAAGCAAACGCACCGTGGCGCTCGATATCAAACAAACGGGCGTGATTCAGGGACTGCCGTTGTTGTT‑CAGCGGCAATAATCTGGTGAAGGCGATTCGCTCTGGAACGCCTAAAGATGCACAAACGCTACGG‑CTGGTGGTCGATCTTACTGAAAACGGTAAAACCGAAGCGGTGAAGCGGc                                                                                                                                                                                                                          >  1:844115/1‑251 (MQ=255)

                         ttCTAACGGTAATCAACAGGCGCGGATAACGTTGAGTTTTATTGGCGATCCTGATTATGCGTTTAGCCAGCAAAGCAAACGCACCGTGGCGCTCGATATCAAACAAACTGGCGTGATTCAGGGACTGCCGTTGTTGTT‑CAGCGGCAATAATCTGGTGAAGGCGATTCGCTCTGGAACGCCTAAAGATGCACAAACGCTACGG‑CTGGTGGTCGATCTTACTGAAAACGGTAAAACCGAATCGGTGAAGCgg                                                                                                                                                                                                                           <  1:723100/250‑1 (MQ=255)

                          tCTAACGGTAATCAACAGGCGCGGATAACGTTGAGTTTTATTGGCGATCCTGATTATGCGTTTAGCCATCAAAGCAAACGCACCGTGGCGCTCGATATCAAACAAACGGGCGTGATTCAGGGACTGCCGTTGTTGTT‑CAGCGGCAATAATCTGGTGAAGGCGATTCGCTCTGGAACGCCTAAAGATGCACAAACGCTACGG‑CTGGTGGTCGATCTTACTGAAAACGGTAAAACCGAAGCGGTGAAGCGGcc                                                                                                                                                                                                                         >  1:221910/1‑250 (MQ=255)

                             aaCGGTAATCAACAGGCGCGGATAACGTTGAGTTTTATTGGCGATCCTGATTATGCGTTTAGCCATCAAAGCAAACGCACCGTGGCGCTGGATATCAAACAAACGGGCGTGATTTAGGGACTGCCGTTGTTGTT‑CAGCGGCAATAATCTGGTGAAGGCGATTCGCTCTGGAACGCCTAAAGATGCACAAACGCTACGG‑CTGGTGGTCGATCTTACGGAAAGCGGTAAAACCGAAGCGGTGAAGCGGCAGa                                                                                                                                                                                                                       <  2:137176/250‑1 (MQ=255)

                             aaCGGTAATCAACAGGCGCGGATAACGTTGAGTTTTATTGGCGATCCTGATTATGCGTTTAGCCATCAAAGCAAACGCACCGTGGCGCTCGATATCAAACAAACGGGCGTGATTCAGGGACTGCCGTTGTTGTT‑CAGCGGCAATAATCTGGTGAAGGCGATTCGCTCTGGAACGCCTAAAGATGCACAAACGCTACGG‑CTGGTGGTCGATCTTACTGAAAACGGTAAAACCGAAGCGGTGAAGCGGCAGa                                                                                                                                                                                                                       >  1:413803/1‑250 (MQ=255)

                              aCGGTAATCAACAGGCGCGGATAACGATGAGTTTTATTGGCGATCCTGATTATGCGTTTAGCCATCAAAGCAAACGCACCGTGGCGCTCGCTATCAAACAAACGGGCGTGATTCAGGGACTGCCGTTGTTGTT‑CAGCGGCAATAATCTGGTGAAGGCGATTAGCTCTGGAACGCCTAAAGATGCACAAACGCTACGG‑CTGGTGGTCGATCTTCCTGAAAAAGGTAAACCCGAAGAGGAGAAggggacgaat                                                                                                                                                                                                                     >  2:863217/1‑242 (MQ=255)

                                   actCAACAGGCGCGGATAACGTTGAGTTTTATTGGCGATCCTGATTATGCGTTTAGCCATCAAAGCAAACGCACCGTGGCGCTCGATATCAAACAAACGGGCGTGATTCAGGGACTGCCGTTGTTGTT‑CAGCGGCAATAATCTGGTGAAGGCGATTCGCTCTGGAACGCCTAAAGATGCACAAACGCTACGG‑CTGGTGGTCGATCTTACTGAAAACGGTAAAACCGAAGCGGTGAAGCGGCAGAATGgcag                                                                                                                                                                                                                <  2:161990/249‑1 (MQ=255)

                                   aaTCAACAGGCGCGGATAACGTTGAGTTTTATTGGCGATCCTGATTATGCGTTTAGCCATCAAAGCAAACGCACCGTGGCGCTCGATATCAAACAAACGGGCGTGATTCAGGGACTGCCGTTGTTGTT‑CAGCGGCAATAATCTGGTGAAGGCGATTCGCTCTGGAACGCCTAAAGATGCACAAACGCTACGG‑CTGGTGGTCGATCTTACTGAAAACGGTAAAACCGAAGCGGTGAAGCGGCAGAATGgca                                                                                                                                                                                                                 <  1:216797/250‑1 (MQ=255)

                                    aTCAACAGGCGCGGATAACGTTGAGTTTTATTGGCGATCCTGATTATGCGTTTAGCCATCAAAGCAAACGCACCGTGGCGCTCGATATCAAACAAACGGGCGTGATTCAGGGACTGCCGTTGTTGTT‑CAGCGGCAATAATCTGGTGAAGGCGATTCGCTCTGGAACGCCTAAAGATGCACAACCGCTACGG‑CTGGTGGTCGATCTTACTGGAAACGGTAAAACCGAAGCGGGGAAGCGGCAGAATGgc                                                                                                                                                                                                                  >  2:598862/1‑248 (MQ=255)

                                         cAGGCGCGGATAACGTTGAGTTTTATTGGCGATCCTGATTATGCGTTTAGCCATCAAAGCAAACGCACCGTGGCGCTCGATATCAAACAAACGGGCGTGATTCAGGGACTGCCGTTGTTGTT‑CAGCGGCAATAATCTGGTGAAGGCGATTCGCTCTGGAACGCCTAAAGATGCACAAACGCTACGG‑CTGGTGGTCGATCTTACTGAAAACGGTAAAACCGAAGCGGTGAAGCGGCAGAATGGCAGCAATTa                                                                                                                                                                                                          <  2:731206/251‑1 (MQ=255)

                                             cgcgGATAACGTTGAGTTATATTGGCGATCCTGATTATGCGTTTAGCCATCAAAGCAAACGCACCGTGGCGCTCGATATCAAACAAACGGGCGTGATTCAGGGACTGCCGTTGTTGTT‑CAGCGGCAATAATCTGGTGAAGGCGATTCGCTCTGGAACGCCTAAAGATGCACAAACGCTACGG‑CTGGTGGTCGGTCTTACTGAAAACGGTAAAACCGAAGCGGAGAAGAGGCCGAATGGCAGCAATTacaat                                                                                                                                                                                                      >  2:466742/1‑249 (MQ=255)

                                              gcgGATAACGTTGAGTTTTATTGGCGATCCTGATTATGCGTTTAGCCATCAAAGCAAACGCACCGTGGCGCTCGATATCAAACAAACGGGCGTGATTCAGGGACTGCCGTTGTTGTT‑CAGCGGCAATAATCTGGTGAAGGCGATTCGCTCTGGAACGCCTAAAGATGCACAAACGCTACGG‑CTGGTGGTCGATCTTACTGAAAACGGTAAAACCGAAGCGGTGAAGCGGCAGAATGGCAGCAATTACACTg                                                                                                                                                                                                     <  1:772357/251‑1 (MQ=255)

                                                 gATAACGTTGAGTTTTATTGGCGATCCTGATTATGCGTTTAGCCATCAAAGCAAACGCACCGTGGCGCTCGATATCAAACAAACGCGCGTGATTCAGGGCCTGCCGTTGTTGTT‑CAGCGGCAATAATCTGGTGAAGGCGATTCGCTCTGGAACGCCTAAAGATGCACAAACGCTACGG‑CTGGTGGTCGATCTTACTGAAAACGGTAAAACCGAAGCGGTGAAGCGGCAGAATGGCAGCAATTACACTgtcg                                                                                                                                                                                                  >  1:486914/1‑251 (MQ=255)

                                                         tGAGTTTTATTGGCGATCCTGATTATGCGTTTAGCCATCAAAGCAAACGCACCGTGGCGCTCGATATCAAACAAACGGGCGTGATTCAGGGACTGCCGTTGTTGTT‑CAGCGGCAATAATCTGGTGAAGGCGATTCGCTCTGGAACGCCTAAAGATGCAAAAACGCTACGG‑CTGGGGGTCGTGCTTACTGAAAACGGTAAAACCGAAGCGGGGAAGCGGCGGAATGGCAGCAATTaccatgtcggcattt                                                                                                                                                                                            >  2:737227/1‑236 (MQ=255)

                                                          gAGTTTTATTGGCGATCCTGATTATGCGTTTAGCCATCAAAGCAAACGCACCGTGGCGCTCGATATCAAACAAACGGGCGTGATTCAGGGACTGCCGTTGTTGTT‑CAGCGGCAATAATCTGGTGAAGGCGATTCGCTCTGGAACGCCTAAAGATGCCCAAACGCTACGG‑CTGGTGGTCGATCTTAATAAAAACGGTAAAACCGAAGCGGTGGGACGGCAGAAAGGCAACAATTACAATGTCGTCT‑TTACg                                                                                                                                                                                         >  2:673136/1‑250 (MQ=255)

                                                              tttATTGTCGATCCTGATTATGCGTTTAGCCAGCAAAGCAAACGCACCGTGGCGTGCGATAGCAAACAAACGGGCGGGATTCAGGGACTGCCGTTGTTGTT‑CAGCGGCAATAATCTGGTGAAGGCGATTCGCGCTGGAACGCCTAAAGATGCACAAACGCTACGG‑CTGGTGGTCGATCTTACTGAAAACGGTAAAACCGAAGCGGTGAAGCGGCAGAATGGCAGCAATTACACTGTCGTCT‑TTACGAttt                                                                                                                                                                                     <  2:538647/250‑2 (MQ=255)

                                                              tttATTGGCGATCCTGATTATGCGTTTAGCCATCAAAGCAAACGCACCGTGGCGCTCGATATCAAACAAACGGGCGTGATTCAGGGACTGCCGTTGTTGTT‑CAGCGGCAATAATCTGGTGAAGGCGATTCGCTCTGGAACGCCTAAAGATGCACAAACGCTACGG‑CTGGTGGTCGATCTTACTGAAAACGGTAAAACCGAAGCGGTGAAGCGGCAGAATGGCAGCAATTACACTGTCGGCT‑TTACGATTa                                                                                                                                                                                     <  2:644489/250‑1 (MQ=255)

                                                                   tGGCGATCCTGATTATGCGTTTAGCCATCAAAGCAAACGCACCGTGGCGCTCGATATCAAACAAACGGGCGTGATTCAGGGACTGCCGTTGTTGTT‑CAGCGGCAATAATCTGGTGAAGGCGATTCGCTCTGGAACGCCTAAAGATGCACAAACGCTACGG‑CTGGTGGTCGATCTTACTGAAAACGGTAAAACCGAAGCGGTGAAGCGGCAGAATGGCAGCAATTACACTGTCGTCT‑TTACGATTAACGcc                                                                                                                                                                                <  2:925149/250‑1 (MQ=255)

                                                                   tGGCGATCCTGATTATGCGTTTAGCCATCAAAGCAAACGCACCGTGGCGCTCGATATCAAACAAACGGGCGTGATTCAGGGACTGCCGTTGTTGTT‑CAGCGGCAATAATCTGGTGAAGGCGATTCGCTCTGGAACGCCTAAAGATGCACAAACGCTACGG‑CTGGTGGTCGATCTTACTGAAAACGGTAAAACCGAAGCGGTGAAGCGGCAGAATGGCAGCAATTACACTGTCGTCT‑TTACGATTAACGcc                                                                                                                                                                                <  1:18319/250‑1 (MQ=255)

                                                                    ggCGAGCCTGATTATGCGTTTAGCCATCAAAGCAAACGCACCGTGGCGCTCGATTTCAAACAAACGTGCGTGATTCAGGGACTGCCGTTGTTGTT‑CAGCGGCAATAATCTGGTGAAGGCGATTCGCTCTGGAACGCCTAAAGATGCACAAACGCTACGG‑CTGGTGGTCGATCTTACTGAAAACGGTAAAACCGAAGCGGTGAAGCGGCAGAATGGCAGCAATTACACTGTCGTCT‑TTACGATTAACGCCg                                                                                                                                                                               <  1:857350/250‑1 (MQ=255)

                                                                       gATCCTGATTATGCGTTTAGCCATCAGAGCAAACGCACCGTGGCGCTCGATATCAAACAAACGGGCGAGATTCAGGGACTGCCGTTGTTGTT‑CAGCGGCAATAATCTGGTGAAGGCGATTCGCTCTGGAACGCCTAAAGATGCACAAACGCTACGG‑CTGGTGGTCGATCTTACTGAAAAAGGTAAAACCGAAGCGGTGCAGCGGCAGAATGGCATCAATTACACTGTAGTCT‑TTACGATTAACGCCGA‑tgg                                                                                                                                                                          >  2:137022/1‑250 (MQ=255)

                                                                        aTCCTGATTATGCGTTTAGCCATCAAAGCAAACGCACCGTGGCGCTCGATATCAAACAAACGGGCGTGATTCAGGGACTGCCGTTGTTGTT‑CAGCGGCAATAATCTGGTGAAGGCGATTCGCTCTGGAACGCCTAAAGATGCACAAACGCTACGG‑CTGGTGGTCGATCTTACTGAAAACGGTAAAACCGAAGCGGTGAAGCGGCAGAATGGCAGCAATTACACTGTCGTCT‑TTACGATTAACGCCGA‑tgtg                                                                                                                                                                         <  2:744526/251‑1 (MQ=255)

                                                                        aTCCTGATTATGCGTTTAGCCATCAAAGCAAACGCACCGTGGCGCTCGATATCAAACAAACGGGCGTGATTCAGGGACTGCCGTTGTTGTT‑CAGCGGCAATAATCTGGTGAAGGCGATTCGCTCTGGAACGCCTAAAGATGCACAAACGCTACGG‑CTGGTGGTCGATCTTACTGAAAACGGTAAAACCGAAGCGGTGAAGCGGCAGAATGGCAGCAATTACACTGTCGTCT‑TTACGATTAACGCCGA‑tgtg                                                                                                                                                                         >  1:933757/1‑251 (MQ=255)

                                                                        aTCCTGATTATGCGTTTAGCCATCAAAGCAAACGCACCGGGGCGCTCGATATCAAACAAACGGGCGTGATTCAGGGACTGCCGTTGTTGTG‑CAGCGGCAATAATCTGGTGAAGGCGATTCGCTCTGGAACGCCTAAAGATGCACAAACGCTACGG‑CTGGTGGTCGATCTTACTGAAAACGGTAAAACCGAAGCGGTGAAGCGGCAGAATGGCAGCAATTACACTGTCGTCT‑TTACGATTAACGCCGA‑tgtg                                                                                                                                                                         <  2:511727/251‑1 (MQ=255)

                                                                            tGATTATGCGTTTAGCCATCAAAGCAAACGCACCGTGGCGCTCGATATCAAACAAACGGGCGTGATTCAGGGACTGCCGTTGTTGTT‑CAGCGGCAATAATCTGGTGAAGGCGATTCGCTCTGGAACGCCTAAAGATGCACAAACGCTACGG‑CTGGTGGTCGATCTTACTGAAAACGGTAAAACCGAAGCGGTGAAGCGGCAGAATGGCAGCAATTACAATGTCGTCT‑TTACGATTAACGCCGA‑TGTgccgc                                                                                                                                                                     >  1:689910/1‑251 (MQ=255)

                                                                            tGATTATGCGTTTAGCCATCAAAGCAAACGCACCGTGGCGCTCGATATCAAACAAACGGGCGTGATTAAGGGACAGCCGTTGTTGTT‑CAGCGGCAATAATCTGGTGAAGGCGATTCGCTCTGGAACGCCTAAAGATGAACAAACGCTACGG‑CTGGTGGTCGATCTTACTGAAAACGGTAAAACCGAAGCGGTGAAGCGGCAGAATGCAAGAAATTACACTGTCAGCT‑TTACGATTAACGCCGA‑tggtccgc                                                                                                                                                                     >  2:675613/1‑245 (MQ=255)

                                                                                tATGCGTTTAGCCATCAAAGCAAACGCACCGTGGCGCTCGATATCAAACAAACGGGCGTGATTCAGGGACTGCCGTTGTTGTT‑CAGCGGCAATAATCTGGTGAAGGCGATTCGCTCTGGAACGCCTAAAGATGCACAAACGCTACGG‑CTGGTGGTCGATCTTACTGAAAACGGTAAAACCGAAGCGGTGAAGCGGCAGAATGGCAGCAATTACACTGTCGTCT‑TTACGATTAACGCCGA‑TGGGCCGCCAcc                                                                                                                                                                 >  2:353509/1‑251 (MQ=255)

                                                                                 aTGCGTTTAGCCATCAAAGCAAACGCACCGTGGCGCTCGATATCAAACAAACGGGCGTGATTCAGGGACTGCCGTTGTTGTT‑CAGCGGCAATAATCTGGTGAAGGCGATTCGCTCTGGAACGCCTAAAGATGCACAAACGCTACGG‑CTGGTGGTCGATCTTACTGAAAACGGTAAAACCGAAGCGGTGAAGCGGCAGAATGGCAGCAATTACACTGTCGTCT‑TTACGATTAACGCCGA‑TGTGCCGCCACCg                                                                                                                                                                >  1:58113/1‑251 (MQ=255)

                                                                                   ccgtgcaGCCATCAAAGCAAACGCGCCGTGGCGCGAGTTATCAAACAAACTGGCGTGATTCAGGGGCTGCCGTTGTTGTT‑CAGGGGCAATAATCTGGTGAATGCGATTCGCTCTGGAACGCCTAAAGATGCACAAACGCGACGG‑CTGGTGGTGGATCGTACTGAAAACGGTAAAACCGACGCGGTGAAGCGGCAGAATGTCAGCAATTACATTGTCGTCT‑TTACGATTATCGCCGA‑TGTGccgccgccgac                                                                                                                                                              <  2:134910/245‑3 (MQ=255)

                                                                                       ttAGCCATCAAAGCAAACGCACCGTGGCGCTCGATATCAAACAAACGGGCGTGATTCAGGGACTGCCGTTGTTGTT‑CAGCGGCAATAATCTGGTGAAGGCGATTCGCTCTGGAACGCCTAAAGATGCACAAACGCTACGG‑CTGGTGGTCGATCTTACTGAAAACGGTAAAACCGAAGCGGTGAAGCGGCAGAATGGCAGCAATTACACTGTCGTCT‑TTACGATTAACGCCGA‑TGTGCCGCCACCGcctcc                                                                                                                                                           <  1:517052/250‑1 (MQ=255)

                                                                                         aGCCATCAAAGCAAACGCACCGTGGCGCTCGATATCAAACAAACGGGCGTGATTCAGGGACTGCCGTGGTTGTT‑CAGCGGCAATAATCTGGTGAAGGCGATTCGCTCTGGAACGCCTAAAGATGCACGAACGCTACGG‑CTGGTGGTCGATCTTACTGAAAACGGTAAAACCGAAGCGGTGAAGCGGCAGAATGGCAGCAATTACACTGTCGTCT‑TTACGATTAACGCCGA‑TGTGCCGCCACCGCCtcctcc                                                                                                                                                        <  1:603569/251‑1 (MQ=255)

                                                                                            cATCAAAGCAAACGAACAGTGGCGCGCGATATCAAACAAACGGGCGTGAGTCAGGGACTGCCGTTTTTTTT‑CAGCGGCAATAATCTGGTGAAGGCGATGCGCTCTGGAACGCCTAAAGATGCACAAACGCGACGG‑CTGGTGGTCGATCTTACTGAAAACGGTAAAACCGAAGCGGTGAAGCGGCAGAATGGCAGCAATTACACTGTCGGCT‑TTACGATTAACGCCGA‑TGTTCCGCCGCCGCCTCCTccgcc                                                                                                                                                     <  2:453842/251‑1 (MQ=255)

                                                                                               cAAAGCAAACGCACCGTGGCGCTCGATATCAAACAAACGGGCGTGATTCAGGGACTGCCGTTGTTGTT‑CAGCGGCAATAATCTGGTGAAGGCGATTCGCTCTGGAACGCCTAAAGATGCACAAACGCTACGG‑CTGGTGGTCGATCTTACTGAAAACGGTAAAACCGAAGCGGTGAAGCGGCAGAATGGCAGCAATTACACTGTCGTCT‑TTACGATTAACGCCGA‑TGTGCCGCCACCGCCTCCTCCgccgcc                                                                                                                                                  >  1:64977/1‑251 (MQ=255)

                                                                                                   gCAAACGCACCGTGGCGCTCGATATCAAACAAACGGGCGTGATTCAGGGACTGCCGTTGTTGTT‑CAGCGGCAATAATCTGGTGAAGGCGATTCGCTCTGGAACGCCTAAAGATGCACAAACGCTACGG‑CTGGTGGTCGATCTTACTGAAAACGGTAAAACCGAAGCGGTGAAGCGGCAGAATGGCAGCAATTACACTGTCGTCT‑TTAAGATTAACGCCGA‑TGTGCCGCCACCGCCTCCTCCCACGCCCGTg                                                                                                                                              >  2:372902/1‑251 (MQ=255)

                                                                                                    cAAACGCACCGTGGCGCTCGATATCAAACAAACGGGCGTGATTCAGGGACTGCCGTTTTTGTT‑CAGCGGCAATAATCTGGTGAAGGCGATTCGCTCTGGAACGCCTAAAGATGCACAAACGCTACGG‑CTGGTGGTCGATCTTACTGAAAACGGTAAAACCGAAGCGGTGAAGCGGCAGAATGGCAGCAATTACACTGTCGTCT‑TTACGATTAACGCCGA‑TGTGCCGCCACCGCCTCCTCCGCCGCCCGTgg                                                                                                                                             <  1:748335/251‑1 (MQ=255)

                                                                                                    cAAACGCACCGTGGCGCTCGATATCAAACAAACGGGCGTGATTCAGGGACTGCCGTTGTTGTT‑CAGCGGCAATAATCTGGTGAAGGCGATTCGCTCTGGAACGCCTAAAGATGCACAAACGCTACGG‑CTGGTGGTCGATCTTACTGAAAACGGTAAAACCGAAGCGGTGAAGCGGCAGAATGGCAGCAATTACACTGTCGTCT‑TTACGATTAACGCCGA‑TGTGCCGCCACCGCCTCCTCCGCCGCCCGTgg                                                                                                                                             >  1:536964/1‑251 (MQ=255)

                                                                                                     aaaCGCACCGAGGCGCTCGATATCAAACACAAGGGCGTGATTCAGGGACTGCCGTTGTTGTT‑CAGCGACCATAATCTGGTGAAGGCGATTCGCTCTGGAACGCCTAAAGATGCACAAACGCTACGG‑CTGGAGGTCGCTCTTACTGAAGACGGTAAAACCGAAGCGGTGAAGCGGCAGAATGGCAGCCCTTACACTGGCGTCT‑TTACGATTAACGCCGA‑TGTGCCGCCACCGCCTCCTCCGCCGCCCTTGG‑t                                                                                                                                           >  2:407329/1‑251 (MQ=255)

                                                                                                       aCGCACCGTGGCGCTCGATATCAAACAAACGGGCGTGATTCAGGGACTGCCGTTGTTGTT‑CAGCGGCAATAATCTGGTGAAGGCGATTCGCTCTGGAACGCCTAAAGATGCACAAACGCTACGG‑CTGGTGGTCGATCTTACTGAAAACGGTAAAACCGAAGCGGTGAAGCGGCAGAATGGCAGCAATTACACTGTCGTCT‑TTACGATTAACGCCGA‑TGGGCCGCCACCGCCTCCCCCGCCGCCCGTgggtt                                                                                                                                          >  2:103186/1‑248 (MQ=255)

                                                                                                         gCACCGTGGCGCTCGATATCAAACAAACGGGCGTGATTCAGGGACTGCCGTTGTTGTT‑CAGCGGCAATAATCTGGTGAAGGCGATTCGCTCTGGAACGCCTAAAGATGCACAAACGCTACGG‑CTGGTGGTCGATCTTACTGAAAACGGTAAAACCGAAGCGGTGAAGCGGCAGAATGGCAGCAATTACACTGTCGTCT‑TTACGATTAACGCCGA‑TGTGCCGCCACCGCCTCCTCCGCCCCCCGTGG‑GTGCg                                                                                                                                       >  2:308518/1‑251 (MQ=255)

                                                                                                              gTGGCGCACGATATCATACAAACGGGGGTGTTTCAGGGCCTGCCGTTGTTGTT‑CAGCGGCAATAAGCTGGTGAAGGCGATTCGCTCTGGACCGCCTAAAGATGCACAAACGCTACGG‑CTGGTGGTCGATCTTACTGAAAACGGTAAACCCGAAGCGGTGAAGCGGCAGAATGGCAGCAATCACACGGTCGTCT‑TTACGATTAACGCCGA‑TGTGCCGCCACCGCCTCCTCCGCCGCCCGTGG‑TTGCGAAAcg                                                                                                                                  <  2:899045/251‑1 (MQ=255)

                                                                                                               tGGCGCTCGATATCAAACAAACGGGCGTGATTCAGGGACTGCCGTTGTTGTT‑CAGCGGCAATAATCTGGTGAAGGCGATTCGCTCTGGAACGCCTAAAGATGCACAAACGCTACGG‑CTGGTGGTCGATCTTACTGAAAACGGTAAAACCGAAGCGGTGAAGCGGCAGAATGGCAGCAATTACACTGTCGTCT‑TTACGATTAACGCCGA‑TGTGCCGCCACCGCCTCCTCCGCCGCCCGTGG‑TTGCGAAAcgc                                                                                                                                 >  1:367217/1‑251 (MQ=255)

                                                                                                               tGGCGCTCGATATCAAACAAACGGGCGTGATTCAGGGACTGCCGTTGTTGTT‑CAGCGGCAATAATCTGGTGAAGGCGATTCACTCTGGAACGCCTAAAGATGCACAAACGCTACGG‑CTGGTGGTCGATCTTACTGAAAACGGTAAAACCGAAGCGGTGAAGCGGCAGAATGGCAGCAATTACAAATTCGTCT‑TTACGATTAACGCCGG‑GTTGCCGCCCCCGCCTCCTCCGCCGCCAGCGG‑CTGCGAACcgc                                                                                                                                 >  2:827798/1‑251 (MQ=255)

                                                                                                                   gcTCGATATCAAACAAACGGGCGTGATTCAGGGACTGCCGTTGTTGTT‑CAGCGGCAATAATCTGGTGAAGGCGATTCGCTCTGGAACGCCTAAAGATGCACAAACGCTACGG‑CTGGTGGTCGATCTTACTGAAAACGGTAAAACCGAAGCGGTGAAGCGGCAGAATGGCAGCAATTACACTGTCGTCT‑TTACGATTAACGCCGA‑TGTGCCACCACCGCCTCCTCCGCCGCCCGTGG‑TTGCGAAACGCGTTg                                                                                                                             >  1:880462/1‑251 (MQ=255)

                                                                                                                      cGATATCAAACAAACGGGCGTGATTCAGGGACTGCCGTTGTTGTT‑CAGCGGCAATAATCTGGTGAAGGCGATTCGCTCTGGAACGCCTAAAGATGCACAAACGCTACGG‑CTGGTGGTCGATCTTACTGAAAACGGTAAAACCGAAGCGGTGAAGCGGCAGAATGGCAGCAATTACACTGTCGTCT‑TTACGATTAACGCCGA‑TGTGCCGCCACCGCCTCCTCCGCCGCCCGTGG‑TTGCGAAACGCGTTGaa                                                                                                                           >  1:499977/1‑250 (MQ=255)

                                                                                                                         tatCGAACAAACGGGCGTGATTCAGGGACTGACGTTGTTGTT‑CAGCGGCAATAATCTGGTGAAGGCGATTCGCTCTGGAACGCCTAAAGATGCACAAACGCTACGG‑CTGGGGGTCGATCTTACTGAAAACGGTAAAACCGAAACGGTGAAGCGGCAGAATGGCAGCAATTACACTGTCGTCT‑TTCCGATTAACGCCGA‑TGGGCCGCCACCGCCACCTCCGCCGCCCCTGG‑TTTCGCAAAGCGTTgcaccac                                                                                                                       >  2:916433/1‑245 (MQ=255)

                                                                                                                          atCAAACAAACGGGCGTGATTCAGGGACTGCCGTTGTTGTT‑CAGCGGCAATAATCTGGTGAAGGCGATTCGCTCTGGAACGCCTAAAGATGCACAAACGCTACGG‑CTGGTGGTCGATCTTACTGAAAACGGTAAAACCGAAGCGGTGAAGCGGCAGAATGGCAGCAATTACACTGTCGTCT‑TTACGATTAACGCCGA‑TGTGCCGCCACCGCCTCCTCCGCCGCCCGTGG‑TTGCGAAACGCGTTGAAACGc‑c                                                                                                                     >  1:657738/1‑251 (MQ=255)

                                                                                                                          atCAAACAAACGGGCGTGATTCAGGGACTGCCGTTGTTGTT‑CAGCGGCAATAATCTGGTGAAGGCGATGCGCTCTGGAACGCCTAAAGATGCACAAACGCTACGG‑CTGGTGGTCGATCTTACTGAAAACGGTAAAACCGAAGCGGTGAAGCGGCAGAATGGCAGCAATTACACTGTCTTCT‑TTACGATTAACGCCGA‑TGTGCCGCCACCGCCTCCTCCGCCGCCCGTGG‑TTGCGAAACGCGTTGAAACGc                                                                                                                       <  1:994232/250‑1 (MQ=255)

                                                                                                                             aaacaaacGGGCGTGATTCAGGGACTGCCGTTGTTGTT‑CAGCGGCAATAATCTGGTGAAGGCGATTCGCTCTGGAACGACTAAAGATGCACAAACGCTACGG‑CTGGTGGTCGATCTTACTGAAAACGGTAAAACCGAAGCGGTGAAGCGGAAGGATGGAAGAACTTACACTGTCGTCT‑TTGCGATTAACGCCGA‑TGTGCAGCCCACGCCTCCTCCGCCGCCCGCGG‑TTGCGAAACGCGTTGAAACGC‑ATGc                                                                                                                  >  2:250017/1‑251 (MQ=255)

                                                                                                                                caaacGGGCGTGTTTCAGTGCCTGCCGTT‑TTGTTACAGCGTCAATAAACTGGTGAAGTCGATTCGCTCTGGAACGCCTAAAGATGCACAAACGCTCCGG‑CTGGTGGTCGATCTTCCTGAAACCGGTAAAACCGAAGCGGTGAAGCGGCAGAATGCCAGCAATTTCACTGTCGTCT‑TTACGATTAACGCCGA‑TGTGCCGCCTCCGCCTCCTCCGCCGCCCGTGG‑TTGCGAAACGCGTTGAAACGC‑CTGCGGt                                                                                                               <  2:729849/251‑1 (MQ=255)

                                                                                                                                caaacGGGCGTGATTCAGGGACTGCCGTTGTTGTT‑CAGCGGCAATAATCTGGTGAAGGCGATTCGCTCTGGAACGCCTAAAGATGCACAACCGCTACGG‑CTGGTGGTCGATCTTACTGAAAACGGTAAAACCGAAGCGGTGAAGCGGCAGAATGGCAGCAATTAAACTGTCGTCT‑TTACGATTAACGCCGA‑TGTGCCGCCACCGCCTCCTCCGCCGCCCGTGGGTTG‑GCAACGCGTTGAAACGC‑CTGCggg                                                                                                               >  2:793737/1‑250 (MQ=255)

                                                                                                                                caaacGGGCGGGATTCAGGGACTGCCGGTGTTGTT‑CAGCGGCAATAATCTGGTGAAAGAGATTCGCTCTGGAACGCCTAAAGATGCACAAACGCTACGG‑CTGGTGAACGATCTTACTGAAAAAGGTAAAACCGAAGCGGTGGAGCGGCAGAATGGCAGCAATTACACTGTCGGCT‑TTACGATTACCACCGG‑TGTTCCGCCCCCCCCCCCTCCTCCGCCCGCGG‑TTGCTAAACGAGTTGAAACAC‑Cttcg                                                                                                                 >  2:177479/1‑246 (MQ=255)

                                                                                                                                aaaccggtcGTGATTCAGGGACTGCCGTTGTTGTT‑CAGCGTCAATAATTGGGTTAAGGCGTTTCGCTCTGGAACGCTTAAAGATGCACAAACGCTTCGG‑CTGGTGGTCGATCTTACTGAAAACGGTAAAACCGAAGCGGTGAAGCGGCATAATGGCAGCAATTCCACTGTCGTCT‑TTACGATTAACGCCGA‑TGGGCCGCCTCCGCCTCCGCCGCCGCCCGTGG‑TTGCGAAACGCGTTGAAACGC‑CTGCGGt                                                                                                               <  2:458521/243‑1 (MQ=255)

                                                                                                                                 aaacGGGCGTGATTCAGGGACTGCCGTTGTTGTT‑CAGCGGCAATAATCTGGTGAAGGCGATTCGCTCTGGAACGCCTAAAGATGCACAAACGCTACGG‑CTGGTGGTCGATCTTACTGAAAACGGTAAAACCGAAGCGGTGAAGCGGCAGAATGGCAGCAATTACACTGTCGTCT‑TTACGATTAACGCCGA‑TGTGCCGCCACCGCCTCCTCCGCCGCCCGTGG‑TTGGGAAACGCGTTGAAACGC‑CTGCGGtt                                                                                                              >  2:55959/1‑251 (MQ=255)

                                                                                                                                    cGGGCGTGATTCAGGGACTGCCGTTGTTGTT‑CAGCGGCAATAATCTGGTGAAGGCGATTCGCTCTGGAACGCCTAAAGATGCACAAACGCTACGG‑CTGGTGGTCGATCTTACTGAAAACGGTAAAACCGAAGCGGTGAAGCGGCAGAATGGCAGCAATTACACTGTCGTCT‑TTACGATTAACGCCGA‑TGTGCCGCCACCGCCTCCTCCGCCGCCCGTGG‑TTGCGAAACGCGTTGAAACGC‑CTGCGGTTGTc                                                                                                           <  1:762318/251‑1 (MQ=255)

                                                                                                                                       gCGTGATTCAGGGACTGCCGTTGTTGTT‑CAGCGGCAATAATCTGGTGAAGGCGATTCGCTCTGGAACGCCTAAAGATGCACAAACGCTACGG‑CTGGTGGTCGATCTTACTGAAAACGGTAAAACCGAAGCGGTGAAGCGGCAGAATGGCAGCAATTACACTGTCGTCT‑TTACGATTAACGCCGA‑TGTGCCGCCACCGCCTCCTCCGCCGCCCGTGG‑TTGCGAAACGCGTTGAAACGC‑CTGCGGTTGTCGc                                                                                                         >  1:280768/1‑250 (MQ=255)

                                                                                                                                        cGTGATTCAGGGACTGCCGTTGTTGTT‑CAGCGGCAATAATCTGGTGAAGGCGATTCGCTCTGGAACGCCTAAAGATGCACAAACGCTACGG‑CTGGTGGTCGATCTTACTGAAAACGGTAAAACCGAAGCGGTGAAGCGGCAGAATGGCAGCAATTACACTGGCGTCT‑TTACGATTAACGCCGACTG‑GCCGCCCCCGCCTCCTCCGCCGCCCGGGG‑TTGCGAAACGCGTTGAAACGC‑CCGCGGGTGTCGCAc                                                                                                       >  2:7318/1‑251 (MQ=255)

                                                                                                                                               cAGGGACTGCCGTTGTTGTT‑CAGCGGCAATAATCTGGTGAAGGCGATTCGCTCTGGAACGCCTAAAGATGCACAAACGCTACGG‑CTGGTGGTCGATCTTACTGAAAACGGTAAAACCGAAGCGGTGAAGCGGCAGAATGGCAGCAATTACACTGTCGTCT‑TTACGATTAACGCCGA‑TGTGCCGCCACCGCCTCCTCCGCCGCCCGTGG‑TTGCGAAACGCGTTGAAACGC‑CTGCGGTTGTCGCACCGCGCGt                                                                                                >  1:782835/1‑251 (MQ=255)

                                                                                                                                                      tGCCGTTGTTGTT‑CAGCGGCACTAATCTGGTGAAGGCGATTCGATCTGGAACGCCTAAAGATGCACAAACGCTACGG‑CTGGAGGTCGCTCTTACTGACAACGGTACAACCGAAGCGGCGAAGCGGCAGAAAGGCAGCAATTTCACTATCGTCT‑TTACGATTAACGCCGC‑TGAGCCCCCACCGCCTCCTCCGCCGCCCGCGG‑TTTCGAAAAGCGTTGAACAGCTCT‑CGGGTGTCGCGCCGCGCGTCacgga                                                                                          >  2:421298/1‑246 (MQ=255)

                                                                                                                                                        ccGTTGTTGTT‑CAGCGGCAATAATCTGGGGAAGGCGATTCGCTCTGGAACGCCTAAAGATGCACAAACGCTACGG‑CTGGTGGTCGATCTTACTGAAAACGGTAAAACCGAAGCGGGGAAGCGGCAGAATGGCAGCAATTACACTGTCGTCT‑TTACGATTAACGCCGA‑TGTGCCGCCACCGCCTCCTCCGCCGCCCGTGG‑TTGCGAAACGCGTTGAAACGC‑CTGCGGTTGTCGCACCGCGCGTCAGCGAAct                                                                                       <  2:818125/251‑2 (MQ=255)

                                                                                                                                                                gtt‑CAGCGGCAATAATCTGGTGAAGGCGATTCGCTCTGGAACGCCTAAAGATGCACAAACGCTACGG‑CTGGTGGTCGATCTTACTGAAAACGGTAAAACCGAAGCGGTGAAGCGGCAGAATGGCAGCAATTACACTGTCGTCT‑TTACGATTAACGCCGA‑TGTGCCGCCACCGCCTCCTCCGCCGCCCGTGG‑TTGCGAAACGCGTTGAAACGC‑CTGCGGTTGTCGCACCGCGCGTCAGCGAACCGgcgcgc                                                                                >  1:261936/1‑250 (MQ=255)

                                                                                                                                                                    cAGCGGCAATAATCTGGTGAAGGCGATTCGCTCTGGAACGCCTAAAGATGCACAAACGCTACGG‑CTGGTGGTCGATCTTACTGAAAACGGTAAAACCGAAGCGGTGAAGCGGCAGAATGGCAGCAATTACACTGTCGTCT‑TTACGATTAACGCCGA‑TGTGCCGCCACCGCCTCCTCCGCCGCCCGTGG‑TTGCGAAACGCGTTGAAACGC‑CTGCGGTTGTCGCACCGCGCGTCAGCGAACCGGCGCGCAATc                                                                            >  1:112977/1‑251 (MQ=255)

                                                                                                                                                                      gCGGCAATAATCTGGTGAAGGCGATTCGCTCTGGAACGCCTAAAGATGCACAAACGCTACGG‑CTGGTGGTCGATCTTACTGAAAACGGTAAAACCGAAGCGGTGAAGCGGCAGAATGGCAGCAATTACACTGTCGTCT‑TTACGATTAACGCCGA‑TGTGCCGCCACCGCCTCCTCCGCCGCCCGTGG‑TTGCGAAACGCGTTGAAACGC‑CTGCGGTTGTCGCACCGCGCGTCAGCGAACCGGCGCGCAATCCg                                                                          <  1:846920/251‑1 (MQ=255)

                                                                                                                                                                      gCGGCAATAATCCGGTGAAGGCGATTCGCTCTGGAACGCCTAAAGACGCACAAACGCTACGG‑CTGGTGGTCGATCTTACGGAAAACGGTAAAACCGAAGCGGTGAAGCGGCAGAATGGCAGCATTTACCCTGTCGTCT‑TTACGATTAACGCCGA‑TGTTCCGCCACCGCCTCCTCCGCCGCCCGTGG‑TTGCGAAACGCGTTGAAACGC‑CTGCGGTTGTCGCACCGCGCGTCAGCGAACCGGCGCGCAATCCg                                                                          <  1:753722/251‑1 (MQ=255)

                                                                                                                                                                       cGGCAATAATCTGGTGAAGGCGATTCGCTCTGGAACGCCTAAAGATGCACAAACGCTACGG‑CTGGTGGTCGATCTTACTGAAAACGGTAAAACCGAAGCGGTGAAGCGGCAGAATGGCAGCAATTACACTGTCGTCT‑TTACGATTAACGCCGA‑TGTGCCGCCACCGCCTCCTCCGCCGCCCGTGG‑TTGCGAAACGCGTTGAAACGC‑CTGCGGTTGTCGCACCGCGCGTCAGCGAACCGGCGCGCAATCCGt                                                                         <  1:365525/251‑1 (MQ=255)

                                                                                                                                                                             taatCTGGTGAAGGCGATTCGCTCTGGAACGCCTAAAGATGCACAAACGCTACGG‑CTGGTGGTCGATCTTACTGAAAACGGTAAAACCGAAGCGGTGAAGCGGCAGAATGGCAGCAATTACACTGTCGTCT‑TTACGATTAACGCCGA‑TGTGCCGCCACCGCCTCCTCCGCCGCCCGTGG‑TTGCGAAACGCGTTGAAACGC‑CTGCGGTTGTCGCACCGCGCGTCAGCGAACCGGCGCGCAATCCGTTTaaac                                                                   >  1:931351/1‑250 (MQ=255)

                                                                                                                                                                              aatCTGGTGAAGGCGATTCGCTCTGGAACGCCTAAAGATGCACAAACGCTACGG‑CTGGTGGTCGATCTTACTGAAAACGGTAAAACCGAAGCGGTGAAGCGGCAGAATGGCAGCAATTACACTGTCGTCT‑TTACGATTAACGCCGA‑TGTGCCGCCACCGCCTCCTCCGCCGCCCGTGG‑TTGCGAAACGCGTTGAAACGC‑CTGCGGTTGTCGCACCGCGCGTCAGCGAACCGGCGCGCAATCCGTTTAAAAc                                                                  >  1:621106/1‑251 (MQ=255)

                                                                                                                                                                                 cTGGTGAAGGCGATTCGCTCTGGAACGCCTAAAGATGCACAAACGCTACGG‑CTGGTGGTCGATCTTACTGAAAACGGTAAAACCGAAGCGGTGAAGCGGCAGAATGGCAGCAATTACACTGTCGTCT‑TTACGATTAACGCCGA‑TGTGCCGCCACCGCCTCCTCCGCCGCCCGTGG‑TTGCGAAACGCGTTGAAACGC‑CTGCGGTTGTCGCACCGCGCGTCAGCGAACCGGCGCGCAATCCGTTTAAAACgg                                                                >  1:70025/1‑250 (MQ=255)

                                                                                                                                                                                   ggTGAAGGCGATTCGCTCTGGAACGCCTCAAGATGCACAAACGCTACGG‑CTGGTGGTCGTTCTTACTGAAAACGGTAAAACCTAAGCGGTGAAGCGGCAGAATGGCAGCAATTACACTGTCGTCT‑TTACGATTAACGCCGA‑TGTGCCGCCACCGCCTCCTCCGCCGCCCGTGG‑TTGCGAAACGCGTTGCAACGC‑CTGCGGTTGTCGCACCCCGCGTCAGCGAACCGGCGCGCAATCCGTTTAAAACGGaa                                                              >  1:751822/1‑250 (MQ=255)

                                                                                                                                                                                      gAAGGCGATTCGCTCTGGAACGCCTAAAGATGCACAAACGCTACGG‑CTGGTGGTCGATCTTACTGAAAACGGTAAAACCGAAGCGGTGAAGCGGCAGAATGGCAGCAATTACACTGTCGTCT‑TTACGATTAACGCCGA‑TGTGCCGCCACCGCCTCCTCCGCCGCCCGTGG‑TTGCGAAACGCGTTGAAACGC‑CTGCGGTTGTCGCACCGCGCGTCAGCGAACCGGCGCGCAATCCGTTTAAAACGGAAAGt                                                           >  1:564139/1‑250 (MQ=255)

                                                                                                                                                                                        aggtgattccccctGGAACTCCGAAAGTTGCACAAACGCTAGGG‑CTGGTGGTCGATCTTTCTGAAAACGATAAAACCGAAGCGGTGAAGCGGCAGAATGGCAGCAATTCCACGGTCGTCT‑TTCCGATTAACGCCGA‑TGGGCCGCCACCGCCTCCTCCGCCGCCCGGGG‑TTGCGAAACGCGGTGAAACGC‑CTGCGGTTGTCGCACCGCGCGTCAGCGAACCGGCGCGCAATCCGTTTAAAACGGAAAGTAAc                                                        <  2:533329/239‑1 (MQ=255)

                                                                                                                                                                                         ggCGAGTCGCTCGGGAAAGCCTAAAGAGGCAAGAACGTTTCGG‑CTGGTGGGCTATCTTACCGAAACCGGTAAAACCGAAGCGGCGATGCGGTAGAATGGCAGCAATCACACTGTCG‑CTGTGACGATTAACGCCGA‑TGTGCCGCCACCGCCTCCTCCGCCGCCCGTGG‑TTGCGAAACGCTTGGAAACGC‑CTGCGGTTGTCGCACCGCGCGTCAGCGAACTGGCGCGCAATCCGTTTAAAACGGAAAGTAAcc                                                       <  2:303316/251‑1 (MQ=255)

                                                                                                                                                                                           cGATGCGCTCGGGACCGCCTAAAGATGCACAAACGCTACGG‑CTGGTGGTAGATCATACTTAAAACGTTAAATCCGAAGCGGGGATGCGGCATAATGGCCGCAATTTCCCTTTCTTCT‑TTACGATTAACGCCGA‑TGCGCCGCCACCGGCTCCTCCGCCGCCCGTGG‑TGGCGAAACGCGTTGAAACGC‑CGGCGGTTGTCGCACCGCGCGTCAGCGAACCTGCGCGCAATCCGGTTAAAACGGAAAGTAACCGc                                                     <  2:807238/251‑1 (MQ=255)

                                                                                                                                                                                            gATTCGCTCTGGAACGCCTAAAGATGCACAAACGCTACGG‑CTGGTGGTCGATCTTACTGAAAACGGTAAAACCGAAGCGGTGAAGCGGCAGAATGGCAGCAATTACACTGTCGTCT‑TTACGATTAACGCCGA‑TGTGCCGCCACCGCCTCCTCCGCCGCCCGTGG‑TTGCGAAACGCGTTGAAACGC‑CTGCGGTTGTCGCACCGCGCGTCAGCGAACCGGCGCGCAATCCGTTTAAAACGGAAAGTAACCGc                                                     >  1:790619/1‑250 (MQ=255)

                                                                                                                                                                                              ttCGCTCTGGAACGCCTAAAGATGCACAAACGCTACGG‑CTGGTGGTCGATCTTACTGAAAACGGTAAAACCGAAGCGGTGAATCGGCAGAATGGCAGCAATTACACTGTCGTCT‑TTACGATTAACGCCGA‑TGTGCCGCCACCGCCTCCTCCGCCGCCCGTGG‑TTGCGAAACGCGTTGAAACTC‑CTGCGGTTGTCGCACCGCGCGTCAGCGAACCGGCGCGCAATCCGTTTAAAACGGAAAGTAACCGCACt                                                  >  1:363712/1‑251 (MQ=255)

                                                                                                                                                                                                cGCTCTGGAACGCCTAAAGATGCACAAACGCTACGG‑CTGGTGGTCGATCTTACTGAAAACGGTAAAACCGAAGCGGTGAAGCGGCAGAATGGCAGCAATTACACTGTCGTCT‑TTACGATTAACGCCGA‑TGTGCCGCCACCGCCTCCTCCGCCGCCCGTGG‑TTGCGAAACGCGTTGAAACGC‑CTGCGGTTGTCGCACCGCGCGTCAGCGAACCGGCGCGCAATCCGTTTAAAACGGAAAGTAACCGCACTAc                                                >  1:469033/1‑251 (MQ=255)

                                                                                                                                                                                                cGCTCTGGAACGACTAACGATGCACAAACGCTACGG‑ATGGCGGTCGCTCTTACTGAAAACGGTAAAACCGAAGCGGAGAAGCGGAAGAATGGCAGCAATTACACAGTCGGCA‑TTACGATTAACGACGA‑TGGGCCGCCACCGCCTCCTCCGCCGCCCGTGG‑TTGCGACACGCGTTGAAACGC‑CTGCGGTTGTAGCACCGCGCGTCAGCGAACCGGCGCGCACTCAGTTTAAAACGGAAAAGAACCGCCCTa                                                 >  2:861930/1‑250 (MQ=255)

                                                                                                                                                                                                 gCTCTGGAACGCCTAAAGATGCACAAACGCTACGG‑CTGGCGGTCGATCTTACTGAAAACGGTAAAACCGAAGCGGTGAAGCGGCAGAATGGCAGCAATTACACTGTCGTCT‑TTCCGATTAACGCCGG‑TGTGCCGCCACCGCCTCCTCCGCCGCCCGTGG‑TTGCGAAACGCGTTGAAACGC‑CTGCGGTTGACGCACCGCGCGTCAGCGAAACGGCGCGACATCCGTTTAAAACGGAAAAGAACCGCACTa                                                 >  2:635083/1‑249 (MQ=255)

                                                                                                                                                                                                    ctGGAACGCCTAAAGATGCACAAACGCTACGG‑CTGGTGGTCGATCTTACTGAAAACGGTAAAACCGAAGCGGTGAAGCGGCAGAATGGCAGCAATTACACTGTCGTCT‑TTACGATTAACGCCGA‑TGTGCCCCCACCGCCTCCTCCGCCGCCCGTGG‑GTGCGAATCGCGTTGAAACGC‑CTGCGGTTGTCGCACCGCGCGTCAGCGAACCGGCGCGCAATCCGGTTAAAACGGAAAGTAACCGCACTACgg                                              <  1:916948/249‑1 (MQ=255)

                                                                                                                                                                                                      ggcaCGCCTTAAGTTGCACAAGCGCTAAGG‑TTTGTGGTCGATCTTACTGAAAACGGTAAAACCGAAGCGGTGAAGCGGCATAATGGCACCAATTCCCCTGTCGTCT‑TTACGATTAACGCCGA‑TTTGCCGCCACCGCCTCCTCCGCCGCCCGTGG‑TTGCGAAACGCGTTGAAACGC‑CTGCGGTTGTCGCACCGCGCGTCAGCGAACCGGCGCGCAATCCGTTTAAAACGGAAAGTAACCGCACTACGGgtg                                           <  2:315118/247‑1 (MQ=255)

                                                                                                                                                                                                      ggAACGCCTAAAGATGCACAAACGCTACGG‑CTGGTGGTCGATCTTACTGAAAACGGTAAAACCGAAGCGGTGAAGCGGCAGAATGGCAGCAATTACACTGTCGTCT‑TTACGATTAACGCCGA‑TGTGCCACCACCGCCTCCTCCGCCGCCCGTGG‑TTGCGAAACGCGTTGAAACGC‑CTGCGGTTGTCGCACCGCGCGTCAGCGAACCGGCGCGCAATCCGTTTAAAACGGAAAGTAACCGCACTACGGgtg                                           <  1:466742/250‑1 (MQ=255)

                                                                                                                                                                                                       gAACGCCTAAAGATGCACAAACGCTACGG‑CTGGTGGTCGATCTTACTGAAAACGGTAAAACCGAAGCGGTGAAGCGGCAGAATGGCAGCAATTACACTGTCGTCT‑TTACGATTAACGCCGA‑TGTGCCGCCACCGCCTCCTCCGCCGCCCGTGG‑TTGCGAAACGCGTTGAAACGC‑CTGCGGTTGTCGCACCGCGCGTCAGCGAACCGACGCGCAATCCGTTTAAAACGGAAAGAAACCGCACTACGGGTGtt                                         >  1:521077/1‑251 (MQ=255)

                                                                                                                                                                                                           gCCTAAATATGCACAAACGCTACGG‑CTGGTGGTCGATCTTACTGAAACCGGGGAAGCCGAAGTGGTGACGCGGCAGACTGGGAGCTTTTGCCTTGTCTTCT‑TTGCGATTACAGCCGT‑TGGACCGCCACCGGCTCCTCCGCCGCCTGTGG‑TTGCGAAACGCGTTGAATCGG‑CTGCGGTGGTCGCACCGTGCGTCAGCGAACCGGCGCGCAACACGTTTAAAACGGAAAGTAACCGCACTACGGGTGGTAtga                                     <  2:701105/251‑3 (MQ=255)

                                                                                                                                                                                                            ccTAAAGATGCACAAACGCTACGG‑CTGGTGGTCGATCTTACTGAAAACGGTAAAACCGAAGCGGTGAAGCGGCAGAATGGCAGCAATTACACTGTCGTCT‑TTACGATTAACGCCGA‑TGTGCCGCCACCGCCTCCTCCGCCGCCCGTGG‑TTGCGAAACGCGTTGAAACGC‑CTGCGGTTGTCGCACCGCGCGTCAGCGAACCGGCGCGCAATCCGTTTAAAACGGAAAGTAACCGCACTACGGGTGTTATcag                                    <  1:55959/251‑1 (MQ=255)

                                                                                                                                                                                                               aaaGATGCACAAACGCTACGG‑CTGGTGGTCGATCTTACTGAAAACGGTAAAACCGAAGCGGTGAAGCGGCAGAATGGCAGCAATTACACTGTCGTCT‑TTACGATTAACGCCGA‑TGTGCCGCCACCGCCTCCTCCGCCGCCCGTGG‑TTGCGAAACGCGTTGAAACGC‑CTGCGGTTGTCGCACCGCGCGTCAGCGAACCGGCGCGCAATCCGTTTAAAACGGAAAGTAACCGCACTACGGGTGTTATcagcag                                 <  1:137022/251‑1 (MQ=255)

                                                                                                                                                                                                               aaaGATGCACAAACGCGACGGTCGGGTG‑TCGATCTTACTGAAAACGGTAAAACCGAAGCGGTGAAGCGGCAGAATGGCAGCATTTCCACTGTCGTCT‑TTACGATTAACGCCGA‑TGTGCCGCCACCGCCTCCTCCGCCGCCCGTGG‑TTGCGAAACGCGTTGAAACGC‑CTGCGGTTGTCGCACCGCGCGTCAGCGAACCGGCGCGCAATCCGTTTAAAACGGAAAGTAACCGCACTACGGGTGTTATcagc                                   <  2:867775/249‑1 (MQ=255)

                                                                                                                                                                                                                       acaACCGCTATGG‑CTGGTGGTCGACCTTACTGGAAACGTTAAACCCGAAGCGGTGAAGCGGCAGAATGGCAGCAATTACACTTTCGTCT‑TTACGATTAACGCCGA‑TGTGCCGCCACCGCCTCCTCCGCCGCCCGTGG‑TTGCGAAACGCGTTGAAACGC‑CTGCGGTTGTCGCACCGCGCGTCAGCGAACCGGCGCGCAATCCGTTTAAAACGGAAAGTAACCGCACTACGGGTGTTATCAGCAGTAATACgg                         <  2:298895/251‑1 (MQ=255)

                                                                                                                                                                                                                        caAACGCTACGG‑CTGGTGGTCGATCTTACTGAAAACGGTAAAACCGAAGCGGTGAAGCGGCAGAATGGCAGCAATTACACTGTCGTCT‑TTACGATTAACGCCGA‑TGTGCCGCCACCGCCTCCTCCGCCGCCCGTGG‑TTGCGAAACGCGTTGAAACGC‑CTGCGGTTGTCGCACCGCGCGTCAGCGAACCGGCGCGCAATCCGTTTAAAACGGAAAGTAACCGCACTACGGGTGTTATCAGCAGTAATACGGt                        <  1:484275/251‑1 (MQ=255)

                                                                                                                                                                                                                         aaaCGCTACGG‑CTGGTGGTCGATCTTACTGAAAACGGTAAAACCGAAGCGGTGAAGCGGCAGAATGGCAGCAATTACACTGTCGTCT‑TTACGATTAACGCCGA‑TGTGCCGCCACCGCCTCCTCCGCCGCCCGTGG‑TTGCGAAACGCGTTGAAACGC‑CTGCGGTTGTCGCACCGCGCGTCAGCGAACCGGCGCGCAATCCGTTTAAAACGGAAAGTAACCGCACTACGGGTGTTATCAGCAGTAATACGGt                        >  1:173198/1‑250 (MQ=255)

                                                                                                                                                                                                                                 cGG‑CTGGTGGGCGATCTTACTGAAAACGGTAAAACCGAAGCGGTGAAGCGGCAGAATGGCAGCAATTACCCTGTCGTCT‑TTACGATTAACGCCGA‑TGTGCCGCCCCCGCCTCCTCCGCCGCCCGTGG‑TTGCGAAACGCGTTGAAACGC‑CTGCGGTTGTCGCACCGCGCGTCAGCGAACCGGCGCGCAATCCGTTTAAAACGGAAAGTAACCGCACTACGGGTGTTATCAGCAGTAATACGGTAACGCGTcc               <  2:423626/251‑1 (MQ=255)

                                                                                                                                                                                                                                       ggtggtCGATCTTACTGAAAACGGTAAAACCGAAGCGGTGAAGCGGCAGAATGGCAGCAATTACACTGTCGTCT‑TTACGATTAACGCCGA‑TGTGCCGCCACCGCCTCCTCCGCCGCCCGTGG‑TTGCGAAACGCGTTGAAACGC‑CTGCGGTTGTCGCACCGCGCGTCAGCGAACCGGCGCGCAATCCGTTTAAAACGGAAAGTAACCGCACTACGGGTGTTATCAGCAGTAATACGGTAACGCGTCCGGCAg          <  1:391127/251‑1 (MQ=255)

                                                                                                                                                                                                                                       gggggacGATCTTAATGAAAACGTTAAAACCGAAGCGGGGAAGCGGCAGAATGGCAGCAATTACACTGTCGTCT‑TTAAGATTAACGCCGA‑TGTGCCGCCCCCGCCTCCTCCGCCGCCCGTGG‑TTGCGAAACGCGTTGAAACGC‑CTGCGGTTGTCGCACCGCGCGTCAGCGAACCGGCGCGCAATCCGTTTAAAACGGAAAGTAACCGCACTACGGGTGTTATCAGCAGTAATACGGTAACGCGTCCGGCAg          <  2:828954/245‑1 (MQ=255)

                                                                                                                                                                                                                                          ggtCGATCTTACTGAAAACGGTAAAACCGAAGCGGTGAAGCGGCAGAATGGCAGCAATTACACTGTCGTCT‑TTACGATTAACGCCGA‑TGTGCCGCCACCGCCTCCTCCGCCGCCCGTGG‑TTGCGAAACGCGTTGAAACGC‑CTGCGGTTGTCGCACCGCGCGTCAGCGAACCGGCGCGCAATCCGTTTAAAACGGAAAGTAACCGCACTACGGGTGTTATCAGCAGTAATACGGTAACGCGTCCGGCAgcgc       <  1:32577/251‑1 (MQ=255)

                                                                                                                                                                                                                                          ggtCGATCTTACTGAAAACGGTAAAACCGAAGCGGTGAAGCGGCAGAATGGCAGCAATTACACTGTAGTCT‑TTACGATTAACGCCGA‑TGTGCCGCCACCGCCTCCTCCGCCGCCCGTGG‑TTGCGAAACGCGTTGACACGC‑CTTCGGTTGTCGCACCGCGCGTCAGCGAACCGGCGCGCAATCCGTTTAAAACGGAAAGTAACCGCACTAAGGGCGTTATCAGCAGTAATCCGCTAACGCGTCCGGccgggc       >  2:760540/1‑246 (MQ=255)

                                                                                                                                                                                                                                               aTCTTACTGAAAACGGTAAAACCGAAGCGGTGAAGCGGCAGAATGGCAGCAATTACACTGTCGTCT‑TTACGATTAACGCCGA‑TGTGCCGCCACCGCCTCCTCCGCCGCCCGTGG‑TTGCGAAACGCGTTGAAACGC‑CTGCGGTTGTCGCACCGCGCGTCAGCGAACCGGCGCGCAATCCGTTTAAAACGGAAAGTAACCGCACTACGGGTGTTATCAGCAGTAATACGGTAACGCGTCCGGCGGcgcgcgcg  >  1:787390/1‑251 (MQ=255)

                                                                                                                                                                                                                                                tCTTACTGAAAACGGTAAAACCGAAGCGGTGAAGCGGCAGAATGGCAGCAATTACACTGTCGTCT‑TTACGATTAACGCCGA‑TGTGCCGCCACCGCCTCCTCCGCCGCCCGTGG‑TTGCGAAACGCGTTGAAACGC‑CTGCGGTTGTCGCACCGCGCGTCAGCGAACCGGCGCGCAATCCGTTTAAAACGGAAAGTAACCGCACTACGGGTGTTATCAGCAGTAATACGGTAACGCGTCCGGCAGcgcgcgcg  >  1:683127/1‑250 (MQ=255)

CCGCGACGCTCTCTGATATTCAGGTTTCTAACGGTAATCAACAGGCGCGGATAACGTTGAGTTTTATTGGCGATCCTGATTATGCGTTTAGCCATCAAAGCAAACGCACCGTGGCGCTCGATATCAAACAAACGGGCGTGATTCAGGGACTGCCGTTGTTGTT‑CAGCGGCAATAATCTGGTGAAGGCGATTCGCTCTGGAACGCCTAAAGATGCACAAACGCTACGG‑CTGGTGGTCGATCTTACCGAAAACGGTAAAACCGAAGCGGTGAAGCGGCAGAATGGCAGCAATTACACTGTCGTCT‑TTACGATTAACGCCGA‑TGTGCCGCCACCGCCTCCTCCGCCGCCCGTGG‑TTGCGAAACGCGTTGAAACGC‑CTGCGGTTGTCGCACCGCGCGTCAGCGAACCGGCGCGCAATCCGTTTAAAACGGAAAGTAACCGCACTACGGGTGTTATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGCG  >  NC_000913/4396129‑4396616

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`