BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,393,552	G→A	F172F (TTC→TTT)	queG ←	epoxyqueuosine reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,393,552	0	G	A	99.1%	358.0 / NA	115	F172F (TTC→TTT)	queG	epoxyqueuosine reductase
Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (48/65); minor base C (1/0); total (49/66)
Fisher's exact test for biased strand distribution p-value = 4.30e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01

Only 100 of 116 total aligned reads displayed.

GGGCAGATAAGCTGGCAGTCATCGCAACCGTAAATACGGTTTCCCATTAACGGTCGCAACTCTTCCGGGATCGCCCCTTCAAGTTCGATGGTGAGATAAGAGATACAGCGGCGAGCATCGACGGTATATGGCTCGACGATGGCACCGGTCGGGCAAATCGTCATACAGGCCACGCATTTGCCGCATCCTTCCTCGACTGGTTGATCCAC‑GGGCAGCGGAATATCGACCAGCAATTCGCCTAAAAAGAAGAACGAACCGGCCTCGCGATTGAGGATAAGTGAGTGCTTACCTGTCCAGCCGAGCCCAGCTTTTTCAGCTAACGGGCGCTCGAGAATAGGCGCAGAATCGACAAACGGTCTAAAATTCAGCGAAACACAATGTTGCTGAATCATCTCGCCCAGCTTTTTGAGTCGGTTGCGCAGAAGTTTGTGATAGTCACGGCCCAGCGCATAACGGCTAACATAGCCGAGTTTGGGGTTTTTCAGCGTG  >  NC_000913/4393307‑4393795

gggCAGATAAGCTGGCAGTCATCGCAACCGTAAATACGGTTTCCCATTAACGGTCGCAACTCTTCCGGGATCGCCCCTTCAAGTTCGATGGTGAGATAAGAGATACAGCGGCGAGCATCGACGGTATATGGCTCGACGATGGCACCGGTCGGGCAAATCGTCATACAGGCCACGCCTTTGCCGCATCCTTCCTCGACTGGTTGTTCCCC‑GGGCCGCGGAATATCGCCCCGCAATTCCCCTAAAaaaaagc                                                                                                                                                                                                                                                 >  2:679174/1‑249 (MQ=255)

   cAGATAAGCTGGCAGTCATCGCAACCGTAAATACGGTTTCCCATTAACGGTCGCAACTCTTCCGGGATCGCCCCTTCAAGTTCGATGGTGAGATAAGAGATACAGCGGCGAGCATCGACGGTATATGGCTCGACGATGGCACCGGTCGGGCAAATCGTCATACAGGCCACGCATTTGCCGCATCCTTCCTCGACTGGTTGATCCAC‑GGGCAGCGGAATATCGACCAGCAATTCGCCTAAAAAAAAgaacg                                                                                                                                                                                                                                              >  1:468457/1‑250 (MQ=255)

    aGATAAGCTGGCAGTCATCGCAACCGTAAATACGGTTTCCCATTAACGGTCGCAACTCTTCCGGGATCGCCCCTTCAAGTTCGATGGTGAGATAAGAGATACAGCGGCGAGCATCGACGGTATATGGCTCGACGATGGCACCGGTCGGGCAAATCGTCATACAGGCCACGCCTTTGCCGCATCCTTCCTCGACTGGTTGATCCAC‑GGGCAGCGGGATATCGACCAGCAATTCGCCTAAAAAAAAgaacga                                                                                                                                                                                                                                             >  2:543093/1‑250 (MQ=255)

        aaGCTTGCAGTCATCGCACCCGTAAATACGGTTTCCCATTAACGGGCGCAACGCTTCCGGGATCGCCCCTTCAAGTTCGATGGTGAGATAAGAGATACAGCTGCGAGCATCGACGGTATATGGCTCGACGATGGCACCGGTCGGGCAAATCGTCATACAGGCCACGCATTTGCCGCATCCTTCCTCGACTGGTAGATCCAC‑GGGCAGCGGAATATCGACCAGCAATTCGCCTAAAAAAAAGAACGAACCgg                                                                                                                                                                                                                                        <  2:632696/251‑1 (MQ=255)

        aaGCTGGCAGTCAGCGCAACCGTAAATACGGTTTCCCATTAACGGTCGCAACTCTTCCGGGAGCGCCCCGTCAAGGTCGATGGTGAGATAAGAGATACAGCGGCGAGCATCGACGGTATATGGCTCGACGATGGCACCGGTCGGGCAAATCGTCATACAGGCCACGCATTTGCCGCATCCTTCCTCGACTGGTTGATCCAC‑GGGCAGCGGAATATCGACCAGCAATTCGCCTAAAAAAAAGAACGAACCg                                                                                                                                                                                                                                         <  2:287309/250‑1 (MQ=255)

          gttGGCAGTAACCGCAACCGTAAATACGGTTTCCCATTAACGGTCGCACCTCTTCCGGGATCGCCCCTTCAGGTTCGATGGTGAGATAAGAGATACAGCGGCGAGAATCGACGGTATATGGCTCAACGATGGCACCGGTCGGGCAAATCGTCATCCAGGCCACGCATTGGCCGCATCCTTCCTCGACTGGTTAATCCCC‑GGGCAGGGGAATACCAACCACAAATTCCCCTAAAAA‑AAGAACGAACCGGCCt                                                                                                                                                                                                                                     <  2:204262/249‑1 (MQ=255)

           cTGGCAGTCATCGCAACCGTAAATACGGTTTCCCATTAACGGTCGCAACTCTTCCGGGATCGCCCCTTCAAGTTCGATGGTGAGATAAGAGATACAGCGGCGCGCATCGACGGTATATGGCTCGACGATGGCACCGGTCGGGCAAATCGTCATACAGGCCACGCATTTGCCGCATCCTTCCTCGACTGGTTGATCCAC‑GGGCACCGGAATATCGACCCACAATTCGCCTAAAAAAAAGAACGAACCGGccc                                                                                                                                                                                                                                     >  2:185547/1‑250 (MQ=255)

           cTGGCAGTCATCGCAACCGTAAATACGGTTTCCCATTAACGGTCGCAACTCTTCCGGGATCGCCCCTTCAAGTTCGATGGTGAGATAAGAGATACAGCGGCGAGCATCGACGGTATATGGCTCGACGATGGCACCGGTCGGGCAAATCGTCATACAGGCCACGCATTTGCCGCATCCTTCCTCGACTGGTTGATCCAC‑GGGCAGCGGAATATCGACCAGCAATTCGCCTAAAAAAAAGAACGAACCGGccc                                                                                                                                                                                                                                     >  2:965713/1‑250 (MQ=255)

               cAGTCATCGCACCCGTAAATACGGTTTCCCATTAACGGTCGCAACTCTTCCGGGATCGCCCCTTCAAGTTCGATGGTGAGATAAGAGATACAGCGGCGAGCATCGACGGGATATGGCTCGACGATGGCACCGATCGGGCAAATCGTCATACAGGCCACGCATTTGCCGCATCCTTCCTCGACTGGTTGATCCAC‑GGGCAGCGGAATATCGACCAGCAATTCGCCTAAAAAAAAGAACGAACCGGCCTcgcg                                                                                                                                                                                                                                 <  2:795415/251‑1 (MQ=255)

               cAGTCATCGCAACCGGAAATACGGTTTCCCATTAACGGCTGCAACTCTTCCGGGATCGCCCCTTCAAGGTCGATGGTGAGATAAGAGATACCGCGGCGAGCATCGACGGGATATGGCTCGACGATGGCACCGGTCGGGCAAATCGTCATACAGGCCACGCATTTGCCGCATCCTTCCTCGACTGGTTGATCCAC‑GGGCAGCGGAATATCGACCAGCAAATCGCCTAAAAAAAAGAACGAACCGGCCTcgcg                                                                                                                                                                                                                                 <  2:290512/251‑1 (MQ=255)

                   cATCGCAACCGTAAATACGGTTTCCCATTAACGGTCGCAACTCTTCCGGGATCGCCCCTTCAAGTTCGATGGTGAGATAAGAGATACAGCGGCGAGCATCGACGGTATATGGCTCGACGATGGCACCGGTCGGGCAAATCGTCATACAGGCCACGCATTTGCCGCATCCTTCCTCGACTGGTTGATCCAC‑GGGCAGCGGAATATCGACCAGCAATTCGCCTAAAAAAAAGAACGAACCGGCCTCGCGATTg                                                                                                                                                                                                                             <  1:389069/251‑1 (MQ=255)

                        cacccGTAAATGCGGTTTCCCATTAACGGGCGCAACTCTTCCGGGATCGCCCCTTCAAGTTCGAGGGTGAGATAAGAGATACAGCGGCGATCATCGACGGTATATGGCTCGACGATGGCACCGGTCGGGCAAATCGTCATACAGGCCACGCATTTGCCGCATCCTTCCTCGACTGGTTGATCCAC‑GGGCAGCGGAATATCGACCAGCAATTCGCCTAAAAAAAAGAACGAACCGGCCTCGCGATTGAGGAt                                                                                                                                                                                                                        <  2:571511/248‑1 (MQ=255)

                        cAACCGTACATACGGTTTCCCATTAACGGTCGCAACTCTTCCGGGATCGCCCCTTCAAGTTCGATGGTGAGATAAGAGATACAGCGGCGAGCATCGACGGTATATGGCTCGTCGATGGCACCGACCGGGCACATCGTCATACAGGCCACGCATTTGCCGCATCCTTCCTCGACTGGTTGATCCAC‑GGGCCGCGGAATATCCCCCAGCAATTCCCCTAAAAAACAGCACGACCCGGCCTCTCGTTTGAGGAt                                                                                                                                                                                                                        >  2:404079/1‑251 (MQ=255)

                          aCCGTAAATACGGTTTCCCATTAACGGTCGCAACTCTTCCGGGATCGCCCCTTCAAGTTCGATGGTGAGATAAGAGATACAGCGGCGAGCATCGACGGTATATGGCTCGACGATGGCACCGGTCGGGCAAATCGTCATACAGGCCACGCATTTGCCGCATCCTTCCTCGACTGGTTGATCCAC‑GGGCAGCGGAATATCGACCAGCAATTCGCCTAAAAAAAAGAACGAACCGGCCTCGCGATTGAGGATaa                                                                                                                                                                                                                      <  1:482515/251‑1 (MQ=255)

                            cGTAAATACGGTTTCCCATTAACGGTCGCAACTCTTCCGGGATCGCCCCTTCAAGTTCGATGGTGAGATAAGAGATACAGCGGCGAGCATCGACGGTATATGGCTCGACGATGGCACCGGTCGGGCAAATCGTCATACAGGCCACGCATTTGCCGCATCCTTCCTCGACTGGTTGATCCAC‑GGGCAGCGGAATATCGACCAGCAATTCGCCTAAAAAAAAGAACGAACCGGCCTCGCGATTGAGGATAagt                                                                                                                                                                                                                    <  1:347189/251‑1 (MQ=255)

                               aaaTACGGTTTCCCATTAACGGTCGCAACTCTTCCGGGATCGCCCCTTCAAGTGCGATGGTGAGAAAAGAGATACAGCGGCGAGCATCGACGGTATATGGCTCGACGATGGCACCGGTCGGGCAAATCGTCATACAGGCCACGCATTTGCCGCATCCTTCCTCGACTGGTTGATCCAC‑GGGCAGCGGAATATCGACCAGCAATTCGCTTAAAAAAAAGAACGAACCGGCCTCGCGATTGAGGATAagtgag                                                                                                                                                                                                                 <  2:715225/251‑1 (MQ=255)

                                       tttCCCATTAACGGTCGCAACTCTTCCGGGATCGCCCCTTCAAGTTCGATGGTGAGATAAGAGATACAGCGGCGAGCATCGACGGTATATGGCTCGACGATGGCACCGGTCGGGCAAATCGTCATACAGGCAACGCATTTGCCGCATCCTTCCTCGACTGGTTGATCCAC‑GGGCAGCGGAATATCGCCCAGCAATTCGCCTAAAAAAAAGAAAGAACCGGCCTCTCGATTGGGGGTAAGTGAGTGCTTaac                                                                                                                                                                                                         >  2:755821/1‑249 (MQ=255)

                                          cccATTAACGGTCGCAACTCTTCCGGGATCGCCCCTTCAAGGTCGATGGTGAGATAAGAGATACAGCGGCGAGCATCGACGGTATATGGCTCGACGATGGCACCGGTCGGGCAAATCGTCATACAGGCCACGCATTTGCCGCATCCTTCCTCGACTGGTTGATCCAC‑GGGCAGCGGAATATCGACCAGCAATTCGCCTAAAAAAAAGAACGAACCGGCCTCGCGATTGAGGATAAGTGAGTGCTTACCTg                                                                                                                                                                                                       <  2:164450/250‑1 (MQ=255)

                                                aaCGGTCGCAACTCTTCCGGGATCGCCCCTTCAAGTTCGATGGTGCGATAAGAGATACAGCGGCGAGCATCGACGGTATATGGCTCGACGATGGCACCGGTCGGGTAAATCGTCATACAGGCCACGCATTTGCCGCATCCTTCCTCGACTGGTTGATCCAC‑GGGCAGCGGAATATCGACCAGCAATTCGCCTAAAAAAAAGAACGAACCGGCCTCGCGATTGAGGATAAGTGAGTGCTTACCTGTCCAGcc                                                                                                                                                                                                <  2:607489/251‑1 (MQ=255)

                                                 actgTCGCAACTCTTCCGGGATAGCCCCTTCAAGTTCGATGGTGAGATAAGAGCTACAGCGGCGAGCATCGACGGTCTATGGCTCGCAGATGGCACCGGTCGGGCAAATCGGCATACAGGCCACGCATTTGCCGCTTCCTTCCTCGACTGTTTTATC‑ACAGGGCAGCGGAATATCGACCAGCAATTCGCCTAAAAAAAAGAACGACCAGTCCTAGCGATTGTGGATAGGTGAGTTATTACTTGTCCacccc                                                                                                                                                                                               >  2:956658/4‑247 (MQ=255)

                                                        cAACTCTTCCGGGATCGCCCCTTCAAGTTCGATGGTGAGATAAGAGATACAGCGGAGAGCATCGACGGTATATGGCTCGACGATGGCACCGGTCGGGCAAATCGTCATAAAGGCCACGCATTTGCCGCATCATTCCTCGACTGGTTGATCCAC‑GGGCAGCGGAATATCGACCAGCAATTCGCCTAAAAAAAAGAACGAACCGGCCTCGCGATTGAGGATAAGTGAGTGCTTACCTGTCCAGCCGAGccc                                                                                                                                                                                          >  1:324391/1‑249 (MQ=255)

                                                          aCTCTTCCGGGATCGCCCCTTCAAGTTCGATGGTGAGATAAGAGATACAGCGGCGAGCATCGACGGTATATGGCTCGACGATGGCACCGGTCGGGCAAATCGTCATACAGGCCACGCATTTGCCGCATCCTTCCTCGACTGGTTGATCCAC‑GGGCAGCGGAATATCGACCAGCAATTCGCCTAAAAAAAAGAACGAACCGGCCTCGCGATTGAGGATAAGTGAGTGCTTACCt                                                                                                                                                                                                        <  1:825207/233‑1 (MQ=255)

                                                          aCTCTTCCGGGATCGCCCCTTCAAGTTCGATGGTGAGATAAGAGATACAGCGGCGAGCATCGACGGTATATGGCTCGACGATGGCACCGGTCGGGCAAATCGTCATACAGGCCACGCATTTGCCGCATCCTTCCTCGACTGGTTGATCCAC‑GGGCAGCGGAATATCGACCAGCAATTCGCCTAAAAAAAAGAACGAACCGGCCTCGCGATTGAGGATAAGTGAGGGCTTACCt                                                                                                                                                                                                        >  2:825207/1‑233 (MQ=255)

                                                             ctTCCGGGATCGCCCCTTCAAGTTCGATGGTGAGATAAGAGATACAGCGGCGAGCATCGACGGTATATGGCTCGACGATGGCACCGGTCGGGCAAATCGTCATACAGGCCACGCATTTGCCGCATCCTTCCTCGACTGGTTGATCCAC‑GGGCAGCGGAATATCGACCAGCAATTCGCCTAAAAAAAAGAACGAACCGGCCTCGCGATTGAGGATAAGTGAGTGCTTACCTGTCCAGCCGAGCCCAGCtt                                                                                                                                                                                     <  1:359398/249‑1 (MQ=255)

                                                                   ggATCGCCCCTCGAAGGTCGATGGTGAGATAAGAGATACAGCGGCGGGCATCGACGGAATAGGGCTCGACGATGGCACCGGTCGGGCAAATCGTCATACAGGCCACGCATTTGCCGCATCCTGCCTCGACTGGTTGATCCAC‑GGGCGGCGGAATATCGACCAGCAATTCGCCTAAAAAAAAGAACGAACCGGCCTCGCGATTGATGATAAGTGAGTGCTTACCTGTCCAGCCGAGCCCAGCTTTTTCAGCt                                                                                                                                                                             <  2:80812/251‑1 (MQ=255)

                                                                    gattgacccTTCAAGTTCGATGGTGAGATAAGAGATACAGCGGCGAGCATCGACGGTATATGGTTCGACTATGGCACCGGTCGGGCAAATCTGCATACAGGCCTCGCATTTGCCGCATCCTTCCTCGACTGGTTGATCCAC‑GGGCAGCGGAATATCGACCAGCAATTCGCCTAAAAAAAAGAACGAACCTGCCCCGCGATTGAGGATAAGTGAGTGCTTACCTGTCCAGCCGAGCCCAGCTTTTTCAGCTa                                                                                                                                                                            <  1:686611/245‑1 (MQ=255)

                                                                       cGCCCCTTCAAGTTCGATGGTGAGATAAGAGATACAGCGGCGAGCATCGACGGTATATGGCTCGACGATGGCACCGGTCGGGCAAATCGTCATACAGGCCACGCATTTGCCGCATCCTTCCTCGACTGGTTGATCCAC‑GGGCAGCGGAATATCGACCAGCAATTCGCCTAAAAAAAAGAACGAACCGGCCTCGCGATTGAGGATAAGTGAGTGCTTACCTGTCCAGCCGAGCCCAGCTTTTTCAGCTAAc                                                                                                                                                                          <  1:278424/250‑1 (MQ=255)

                                                                        gCCCATTCAAGTTCGATGGTGAGATAAGAGATACAGCGGCGAGCATCGACGGTATATGGCTCGACGATGGCACCGGTCGGGCAAATCGTCATACAGGCCACGCATTTGCCGCATCCTTCCTCGACTGGTTGATCCAC‑GGGCAGCGGAATATCGACCATCAATTCGCCTAAAAAAAAGAACGAACCGGCCTCGCGATTGAGGATAAGTGAGTGCTTACCTGTCCAGCCGAGCCCAGCTTTTTCAGCTAACgg                                                                                                                                                                        >  1:680914/1‑251 (MQ=255)

                                                                         ccccTTCAAGTTAGATGGTGAGATAAGAGATACAGCGGCGAGCATCGACGGTATATGGCTCGACGATGGCACCGGTCGGGCAAATCGTCATACAGGCCACGCATTTGCCGCATCCTTCCTCGACTGGTTGATCCAC‑GGGCAGCGGAATATCTACCAGCAATTCGCCTAAAAAAAAGAACGAACCGGCCTCGCGATTGAGGATAAGTGAGTGCTTACCTGTCCAGCCGAGCCCAGCTTTTTCAGCTAACggg                                                                                                                                                                       >  1:791564/1‑251 (MQ=255)

                                                                               cAAGTTCGATGGTGAGATAAGAGATACAGCGGCGAGCATCGACGGTATATGGCTCGACGATGGCACCGGTCGGGCAAATCGTCATACAGGCCACGCATTTGCCGCATCCTTCCTCGACTGGTTGATCCAC‑GGGCAGCGGAATATCGACCAGCAATTCGCCTAAAAAAAAGAACGAACCGGCCTCGCGATTGAGGATAAGTGAGTGCTTACCTGTCCAGCCGAGCCCAGCTTTTTCAGCTAACGGGCGCTCg                                                                                                                                                                 <  1:197242/251‑1 (MQ=255)

                                                                               cAAGGTCGATGGTGAGATAAGAGATACAGCGGCGAGCATCGACGGTATATGGCTCGACGATGGCACCGGTCGGGCAAATCGTCATACAGGCCACGCATTTGCCGCATCCTTCCTCGACTGGTTGATCCAC‑GGGCAGCGGAATATCGACCAGCAATTCGCCTAAAAAAAAGAACGAACCGGCCTCGCGATTGAGGATAAGTGAGTGCTTACCTGTCCAGCCGAGCCCAGCTTTTTCAGCTAACGGGCGCTc                                                                                                                                                                  <  2:409336/250‑1 (MQ=255)

                                                                                aaGTTCGATGGTGAGATAAGAGATACAGCGGCGAGCATCGACGGTATATGGCTCGACGATGGCACCGGTCGGGCAAATCGTCATACAGGCCACGCATTTGCCGCATCCTTCCTCGACTGGTTGATCCAC‑GGGCAGCGGAATATCGACCAGCAATTCGCCTAAAAAAAAGAACGAACCGGCCTCGCGATTGAGGATAAGTGAGTGCTTACCTGTCCAGCCGAGCCCAGCTTTTTCAGCTAACGGGCGCTCgg                                                                                                                                                                >  1:943633/1‑250 (MQ=255)

                                                                                 aGTTCGATGGTGAGATAAGAGATACAGCGGCGAGCATCGACGGTATATGGCTCGACGATGGCACCGGTCGGGCAAATCGTCATACAGGCCACGCATTTGCCGCATCCTTCCTCGACTGGTTGATCCAC‑GGGCAGCGGAATATCGACCAGCAATTCGCCTAAAAAAAAGAACGAACCGGCCTCGCGATTGAGGATAAGTGAGTGCTTACCTGTCCAGCCGAGCCCAGCTTTTTCAGCTAACGGGCGCTCg                                                                                                                                                                 >  2:331674/1‑249 (MQ=255)

                                                                                  gTTCGATGGTGAGATAAGAGATACAGCGGCGAGCATCGACGGTATATGGCTCGACGATGGCACCGGTCAGGCAAATCGTCATACAGGCCACGCATTTGCCGCATCCTTCCTCGACTGGTTGATCCAC‑GGGCAGCGGAATATCGACCAGCAATTCGCCTAAAAAAAAGAACGAACCGGCCTCGCGATTGAGGATAAGTGAATGCTTACCTGTCCATCCGAGCCAAGCTTTTTCACCTAACGGgcgcccg                                                                                                                                                                 >  1:884640/1‑245 (MQ=255)

                                                                                   ttCGATGGTGAGATAAGAGATACAGCGGCGAGCATCGAGGGTATATGGCTCGACGATGGCACCGGTCGGGAAAATCGTCATACAGGACACGCCTTTGCCGCATCCTTCCTCGACTGGTTGATCCAA‑GGGCAGCGGAATATCGACCAGCAATTCGCCTAAAAAAAAGACCGAACCGGCCTCGCGATTGAGGATACGAGAGTGATAACCTGTCAAGCCGAGCCCCGATTTTTGAGTTAAAGGGCGCTCgg                                                                                                                                                                >  2:597707/1‑247 (MQ=255)

                                                                                        tGGTGAGATAAGAGATACAGCGGCGAGCATCGACGGTATATGGCTCGACGATGGCACCGGTCGGGCAAATCGTCATACAGGCCACGCATTTGCCGCATCCTTCCTCGACTGGTTGATCCCC‑GGGCAGCGGAATATCGACCAGCAATTCGCCTAAAAAAAAGAACGAACCGGCCTCGCGATTGCGGATAAGTGAGTGCTTACCTGTCCAGCCGAGCCCAGCTTTTTCAGCTAACGGGCGCTCGAGAAtcggg                                                                                                                                                        >  1:631721/1‑247 (MQ=255)

                                                                                         ggTGAGATAAGAGATACAGCGGCGAGCATCGACGGTATATGGCTCGACGATGGCACCGGTCGGGCAAATCGTCATACAGGCCACGCATTTGCCGCATCCTTCCTCGACTGGTTGATCCAC‑GGGCAGCGGAATATCGACCAGCAATTCGCCTAAAAAAAAGAACGAACCGGCCTCGCGATTGAGGATAAGTGAGTGCTTACCTGTCCAGCCGAGCCCAGCTTTTTCAGCTAACGGGCGCTCGAGAATAGgcg                                                                                                                                                       >  1:103748/1‑251 (MQ=255)

                                                                                          gggcaaTAAGAGATACAGCGGCGAGCATCGACGGTATATGGCTCGTCGATGGCACCGGGCGGGCAAATCGTCATACAGGCCACGCATGTGCCGAAGCCTGCCTCGACTGGTGGAGCCAC‑GGGCAGCGGAATATCGACCAGCAATTCGCCTAAAAAAAAGAACGAACCGGCCTCGCGAGTGAGGATAAGTGAGTGCTTACCTGTCCAGCCGAGCCCAGCTTTTTCAGCTAACGGGCGCTCGAGAATAGgcgc                                                                                                                                                      <  2:434221/246‑1 (MQ=255)

                                                                                               aTAAGAGGTACAGCGGCGAGCATCGACGGTATATGGCTCGACGATGGCACCGGTCGGGCAAATCGTCATACAGGCCACGCATTTGCCGCATCCTTCCTCGACTGGTTGATCCAC‑GGGCAGCGGAATATCGACCAGCAATTCGCCTAAAAAAAAGAACGAACCGGCCTCGCGATTGAGGATAAGTGAGTGCTTACCTGTCCAGCCGAGCCCAGCTTTTTCAGCTAACGGGCGCTCGAGAATAGGCGCAGAAt                                                                                                                                                 <  1:913573/251‑1 (MQ=255)

                                                                                                 aaGAGATACAGCGGCGAGCATCGACGGTATATGGCTCGACGATGGCACCGGTCGGGCAAATCGTCATACAGGCCACGCATTTGCCGCATCCTTCCTCGACTGGGTGATCCAC‑GGGCAGCGGAATATCGACCAGCAATTCGCCTAAAAAAAAGAACGAACCGGCCTCGCGATTGAGGATAAGTGAGTGCTTACCTGTCCAGCCGAGCCCAGCTTTTTCAGCTAACGGGCGCTCGAGAATAGGCGCAGAATc                                                                                                                                                <  2:605231/250‑1 (MQ=255)

                                                                                                  agagATACAGCGGCGAGCATCGACGGTATATGGCTCGACGATGGCACCGGTCGGGCAAATCGTCATACAGGCCACGCATTTGCCGCATCCTTCCTCGACTGGTTGATCCAC‑GGGCAGCGGAATATCGACCAGCAATTCGCCTAAAAAAAAGAACGAACCGGCCTCGCGATTGAGGATAAGTGAGTGCTTACCTGTCCAGCCGAGCCCAGCTTTTTCAGCTAACGGGCGCTCGAGAATAGGCGCAGAATCg                                                                                                                                               >  1:314887/1‑250 (MQ=255)

                                                                                                   gagaTACAGCGGCGAGCATCGACGGTATATGGCTCGACGATGGCACCGGTCGGGCAAATCGTCATACAGGCCACGCATTTGCCGCATCCTTCCTCGACTGGTTGATCCAC‑GGGCAGCGGAATATCGACCAGCAATTCGCCTAAAAAAAAGAACGAACCGGCCTCGCGATTGAGGATAAGTGAGTGCTTACCTGTCCAGCCGAGCCCAGCTTTTTCAGCTAACGGGCGCTCGAGAATAGGCGCAGAATCGAc                                                                                                                                             <  1:274130/251‑1 (MQ=255)

                                                                                                      aTACAGCGGCGAGCATCGACGGTATATGTCTCGACGATGGCACCGGTCGGGCAAATCGTCATACAGGCCACGCATTTGCCGCATCCTTCCTCGACTGGTTGATCCAC‑GGGCAGCGGAATATCGACCAGCAATTCGCCTAAAAAAAAGAACGAACCGGCCGCGCGATTGAGGAGAAGTGAGTGCTTACCTGTCTAGCCGAGCCCAGCTTTTTCAGCTAACGGGCGCTCGAGAATAGGCGCAGAATCGACaaa                                                                                                                                          <  1:623659/251‑1 (MQ=255)

                                                                                                       tACAGCGGCGAGCATCGACGGTATATGGCTCGACGATGGCACCGGTCGGGCAAATCGTCATACAGGCCACGCATTTGCCGCATCCTTCCTCGACTGGTTGATCCAC‑GGGCAGCGGAATATCGACCAGCAATTCGCCTAAAAAAAAGAACGAACCGGCCTCGCGATTGAGGATAAGTGAGTGCTTACCTGTCCAGCCGAGCCCAGCTTTTTCAGCTAACGGGCGCTCGAGAATAGGCGCAGAATCGACAAAc                                                                                                                                         >  1:486094/1‑251 (MQ=255)

                                                                                                        aCAGCGGCGAGCATCGACGGTATATGGCTCGACGATGGCACCGGTCGGGCAAATCGTCATACAGGCCACGCATTTGCCGCATCCTTCCTCGACTGGTTGATCCAC‑GGGCAGCGGAATATCGACCAGCAATTCGCCTAAAAAAAAGAACGAACCGGCCTCGCGATTGAGGATAAGTGAGTGCTTACCTGTCCAGCCGAGCCCAGCTTTTTCAGCTAACGGGCGCTCGAGAATAGGCGCAGAATCGACAAAc                                                                                                                                         <  1:697191/250‑1 (MQ=255)

                                                                                                        aCAGCGGCGAGCATCGACGGTATATGGCTCGACGATGGCACCGGTCGGGCAAATCGTCATACAGGCCACGCATTTGCCGCATCCTTCCTCGACTGGTTGATCCAC‑GGGCAGCGGAATATCGACCAGCAATTCGCCTAAAAAAAAGAACGAACCGGCCTCGCGATTGAGGATAAGTGAGTGCTTACCTGTCCAGCCGAGCCCAGCTTTTTCAGCTAACGGGCGCTCGAGAATAGGCGCAGAATCGACAAACg                                                                                                                                        <  1:760058/251‑1 (MQ=255)

                                                                                                        aCAGCGGCGAGCATCGACGGTATATGGCTCGACGATGGCACCGGTCGGGCAAATCGTCATACAGGCCACGCAATTGCCGCATCCTTCCTCGACAGGTTGATCCAC‑GGGCAGCGGAAAATCGACCAGCAATTCGCCTAAAAAAAAGAACGACACGGCCTCGCGGTTGAGGATAAGTGAGTGCTTACATGTCCAGCCGGGCCCAGCTTTTTCAGCTAACGGGCGCTCGGGACAAGGCGCAgcaaagacaag                                                                                                                                          >  2:545474/1‑239 (MQ=255)

                                                                                                          aGCGGCGAGCATAGACGGTATATGGCTCGACGATGGCACCGGTCGGGCAAATCGTCATACAGGCCACGCATTTGCCGCATCCTTCCTCGACTGGTTGATCCAC‑GGGCAGCGGAATATCGACCAGCAATTCGCCTAAAAAAAAGAACGAACCGGCCTCGCGATTGCGGATAAGTGCGTGCTTACCTGTCCAGCCGAGCCCAGCTTTTTCAGCTAACGGGCGCTCGGGAATAGGCGCAGAATCGACAAACGGt                                                                                                                                      >  2:717018/1‑251 (MQ=255)

                                                                                                           gcggcgAGCATCGACGGTATATGGCTCGACGATGGCACCGGTCGGGCAAATCGTCATACAGGCCACGCATTTGCCGCATCCTTCCTCGACTGGTTGATCCAC‑GGGCAGCGGAATATCGACCAGCAATTCGCCTAAAAAAAAGAACGAACCGGCCTCGCGATTGAGGATAAGTGAGTGCTTACCTGTCCAGCCGAGCCCAGCTTTTTCAGCTAACGGGCGCTCGAGAATAGGCGCAGAATCGACAAACGGTc                                                                                                                                     >  1:499239/1‑251 (MQ=255)

                                                                                                                gAGCATCGACGGTATATGGCTCGACGATGGCACCGGTCGGGCAAATCGTCATACAGGCCACGCATTTGCCGCATCATTCCTCGACTGGTTGATCCAC‑GGGCAGCGGAATATCGACCAGCAATTCGCCTAAAAAAAAGAACGAACCGGCCTCGCGATTGAGGATAAGTGAGTGCTTACCTGTCCAGCCGAGCCCAGCTTTTTCAGATAACGGGCGCTCGAGAAAAGGCGACGAATCGACACACGGTCTaaa                                                                                                                                 >  2:342715/1‑250 (MQ=255)

                                                                                                                 aGCATCGACGGTATATGGCTCGACGATGGCACCGGTCGGGCAAATCGTCATACAGGCCACGCATTTGCCGCATCCTTCCTCGACTGGTTGATCCAC‑GGGCAGCGGAATATCGACCAGCAATTCGCCTAAAAAAAAGAACGAACCGGCCTCGCGATTGAGGATAAGTGAGTGCTTACCTGTCCAGCCGAGCCCAGCTTTTTCAGCTAACGGGCGCTCGAGAATAGGCGCAGAATCGACAAACGGTCTAAAAt                                                                                                                               <  1:543093/251‑1 (MQ=255)

                                                                                                                    aTCGACGTTATATGGCTCGACGATGGCACCGGTCGGGCAAATCGTCATACAGGCCACGCATTTGCAGCATCCTTCCTCGACTGGTTGATCCAC‑GGGCAGCGGAATATCGACCAGCAATTCGCCTAAAAAAAAGAACGAACCGGCCTCGCGATTGAGGATAAGTGAGGGCTTACCTGTCCAGCCTAGCCCAGCTTTTTCAGCTAAAGGTCGCAGGAGGATAGGGGCAGATGCGACAAACgggataaattta                                                                                                                             >  2:747378/1‑240 (MQ=255)

                                                                                                                    aTCGACGGTATATGGCTCGACGATGGCACCGGTCGGGCAAATCGTCATACAGGCCACGCATTTGCCGCATCCTTCCTCGACTGGTTGATCCAC‑GGGCAGCGGAATATCGACCAGCAATTCGCCTAAAAAAAAGAACGAACCGGCCTCGCGATTGAGGATAAGTGAGTGCTTACCTGTCCAGCCGAGCCCAGCTTTTTCAGCTAACGGGCGCTCGAGAATAGGCGCAGAATCGACAAACGTTCTAAAATTc                                                                                                                             >  1:678422/1‑250 (MQ=255)

                                                                                                                      cGACGGTATATGGCTCGACGATGGCACCGGTCGGGCAAATCGTCATACAGGCCGCGCATTTGCCGCATCCTTCCTCGACTGGTTGATCCAC‑GGGCAGCGGAATATCGACCAGCAATTCGCCTAAAAAAAAGAACGAACCGGCCTCGCGATTGAGGATAAGTGAGTGCTTACCTGTCCAGCCGAGCCCAGCTTTTTCAGCTAACGGGCGCTCGAGAATAGGCGCAGAATCGACAAACGGTCTAAAATTCAg                                                                                                                           <  1:554945/250‑1 (MQ=255)

                                                                                                                      cGACGGTATATGGCTCGACGATGGCACCGGTCGGGCAAATCGTCATACAGGCCACGCATTTGCCGCATCCTTCCTCGACTGGTTGATCCAC‑GGGCAGCGGAATATCGACCAGCAATTCGCCTAAAAAAAAGAACGAACCgg                                                                                                                                                                                                                                        >  2:47746/1‑141 (MQ=255)

                                                                                                                      cGACGGTATATGGCTCGACGATGGCACCGGTCGGGCAAAGCGTCATACAGGCCACGCATTTGCCGCATCCTTCCTCGACTGGTTGATCCAC‑GGGCAGCGGAATATCGACCAGCAATTCGCCTAAAAAAAAGAACGAACCgg                                                                                                                                                                                                                                        <  1:47746/141‑1 (MQ=255)

                                                                                                                       aaCGGTATATGGCTAGCCGATGGCACCGGTCGGGCAAATCGGCATACAGGCCACGCATTTGCAGCATCCTTCCTCGACTGGTTGATCCAC‑GGGCAGCGGAATATCGAACAACAATTCGCCTAAAAAAAAGAACGAACCGGCCTCGCGAGTGGGGATAAGTGAGTGCTTACCTGTACAGCCGAGCCCAGATTTTTCATCTAACGGGCGCTCGCGAATAGGCGCAGAATCTACAAACGTTATAAAATTCAGCg                                                                                                                         >  2:602103/2‑251 (MQ=255)

                                                                                                                             atatGGCTCGACGATGGCACCGGTCGGGCAAATCGTCATACAGGCCACGCATTTGCCGCATCCTTCCTCGACTGGTTGATCCAC‑GGGCAGCGGAATATCGACCAGCAATTCGCCTAAAAAAAAGAACGAACCGGCCTCGCGATTGAGGATAAGAGAGTGCTTACCTGTCCAGCCGAGCCCAGCTTTTTCAGCTAACGGGCGCTCGAGAATAGGCGCAGAATCGAAACACGGACTAAAATTCAGCGAAacc                                                                                                                    >  2:425897/1‑249 (MQ=255)

                                                                                                                               atGGCTCGACGATGGCACCGGTCGGGCAAATCGTCATACAGGCCACGCATTTGCCGCATCCTTCCTCGACTGGTTGATCCAC‑GGGCAGCGGAATATCGACCAGCAATTCGCCTAAAAAAAAGAACGAACCGGCCTCGCGATTGAGGATAAGTGAGTGCTTACCTGTCCAGCCGAGCCCAGCTTTTTCAGCTAACGGGCGCTCGAGAATAGGCGCAGAATCGACAAACGGTCTAAAATTCAGCGAAACACaa                                                                                                                 <  1:99554/251‑1 (MQ=255)

                                                                                                                                  gCTCGACGATGGCACCGGTCGGGCAAATCGTCATACAGGCCACGCATTTGCCGCATCCTTCCTCGACTGGTTGATCCAC‑GGGCAGCGGAATATCGACCAGCAATTCGCCTAAAAAAAAGAACGAACCGGCCTCGCGATTGAGGATAAGTGAGTGCTTACCTGTCCAGCCGAGCCCAGCTTTTTCAGCTAACGGGCGCTCGAGAATAGGCGCAGAATCGACAAACGGTCTAAAATTCAGCGAAACACAATGt                                                                                                              <  1:637703/251‑1 (MQ=255)

                                                                                                                                    ccgacgaTGGCACCGGTCGGGCAAATCGTCATACAGGCCACGCATTTGCCGCATCCGTCCTCGACGGGTTGATCCAC‑GGGCAGCGGAATATCGACCAGCAATTCGCCTAAAAAAAAGAACGAACCGGCCTCGCGATTGAGGATAAGTGAGTGCTTACCTGTCCAGCCGAGCCCAGCTTTTTCAGCTAACGGGCGCTCGAGAATAGGCGCAGAATCGACAAACGGTCTAAAATTCAGCGAAACACAATGTTg                                                                                                            <  1:884587/250‑1 (MQ=255)

                                                                                                                                     cgacgaTGGCCCCGGGCGGGCAAAGCGTCATACAGGCCACGCATTTGCCGCATCCTTCCTCGGCTGGTTGATCCAC‑GGGCAGCGGAATATCGACCAGCAATTCGCCTAAAAAAAAGAACGAACCGGCCTCGCGATTGAGGATAAGTGAGTGCTTACCTGTCCAGCCGAGCCCAGCTTTTTCAGCTAACGGGCGCTCGAGAATAGGCGCAGAATCGACAAACGGTCTAAAATTCAGCGAAACACAATGTTGc                                                                                                           <  2:709583/251‑1 (MQ=255)

                                                                                                                                       acgaTGGCACGGGTAGGGCAAATCGTCATACAGGCCACGCATTTGCCGAATCCTTCCTCGAATGGTTGCTCCAC‑GGGCAGCGGAATATAGACCAGCAATTCGCCTAAAAAAAAGAACGAACCGGCCTCGCGATTGAGGATAAGTGAGTGATTACCTATTCAGCAGAGCCCAGCTTTTTCAGCTAACGGGCGCTCGAGAAAAGTAGCAGCATAGACGAACGGACTAACATTCAGCGAGAcacacaattgctg                                                                                                         >  2:34504/1‑242 (MQ=255)

                                                                                                                                                ccgggtgggCAAATCGTCATACAGGCCACGCATTTGCCGCCCCCTTCCTCGACTGGTTGAACCGC‑GGGCAGCGGAATATCGACCAGCAATTCGCCTAAAAAAAAGAACGAACCGGCCTCGCGATTGAGGATAAGTGAGTGCTTACCTGTCCAGCCGAGCCCAGCTTTTTCAGCTAACGGGCGCTCGAGAATAGGCGCAGAATCGACAAACGGTCTAAAATTCAGCGAAACACAATGTTGCTGAATCAtctc                                                                                                <  2:246850/245‑1 (MQ=255)

                                                                                                                                                ccGGTCGGGCAAATCGTCATACAGGCCACGCATTTGCCGCATCCTTCCTCGACTGGTTGATCCAC‑GGGCAGCGGAATATCGACCAGCAATTCGCCTAAAAAAAAGAACGAACCGGCCTCGCGATTGAGGATAAGTGAGTGCTTACCTGTCCAGCCGAGCCCAGCTTTTTCAGCTAACGGGCGCTCGAGAATAGGCGCAGAATCGACAAACGGTCTAAAATTCAGCGAAACACAATGTTGCTGAATCAtctc                                                                                                <  1:627491/251‑1 (MQ=255)

                                                                                                                                                 cgttCGGGCAAATCGGCAGACAGGCCACGCATGTGCCGCATCCTTTCTCGACTGGTTGATCCAC‑TGGCAGCGGAATATCGACCAGCAAGTCGCCTAAAAAAAAGAACGAACCGGCCTCGCGATTGAGGATAAGTGAGTGCTTACCGGTCCAGCCGAGCCCAGCTTTTTCAGCTAACGGGCGCTCGAGAATAGGCGCAGAATCGACAAACGGTCTAAAATTCAGCGAAACACAATGTTGCTGAATCATCTCg                                                                                               <  2:891233/248‑1 (MQ=255)

                                                                                                                                                 cGGTCGGGCAAATCGTCATACAGGCCACGCATTTGCCGCATCCTTCCTCGACTGGTTGATCCAC‑GGGCAGCGGAATATCGACCAGCAATTCGCCTAAAAAAAAGAACGAACCGGCCTCGCGATTGAGGATAAGTGAGTGCTTACCTGTCCAGCCGAGCCCAGCTTTTTCAGCTAACGGGCGCTCGAGAATAGGCGCAGAATCGACAAACGGTCTAAAATTCAGCGAAACACAATGTTGCTGAATCATCTCg                                                                                               <  1:353120/251‑1 (MQ=255)

                                                                                                                                                 cGGTCGGGCAAATCGTCATACAGGCCACGCATTTGCCGCATCCTTCCTCGACTGGTTGATCCAC‑GGGCAGCGGAATATCGACCAGCAATTCGCCTAAAAAAAAGAACGAACCGGCCTCGCGATTGAGGATAAGTGAGTGCTTACCTGTCCAGCCGAGCCCAGCTTTTTCAGCTAACGGGCGCTCGAGAATAGGCGCAGAATCGACAAACGGTCTAAAATTCAGCGAAACACAATGTTGCTGAATCATCTCg                                                                                               <  1:418444/251‑1 (MQ=255)

                                                                                                                                                   gTCGGGCAAATCGTCATACAGGCCACGCATTTGCCGCATCCTTCCTCGACTGGTTGATCCAC‑GGGCAGCGGAATATCGACCAGCAATTCGCCTAAAAAAAAGAACGAACCGGCCTCGCGATTGAGGATAAGTGAGTGCTTACCTGTCCAGCCGAGCCCAGCTTTTTCAGCTAACGGGCGCTCGAGAATAGGCGCAGAATCGACAAACGGTCTAAAATTCAGCGAAACACAATGTTGCTGAATCATCTCGcc                                                                                             >  2:259149/1‑251 (MQ=255)

                                                                                                                                                   gTCGGGCAAATCGTCATACAGGCCACGCATTTGCCGCATCCTTCCTCGACTGGTTGATCCAC‑GGGCAGCGGAATATCGACCAGCAATTCGCCTAAAAAAAAGAACGAACCGGCCTCGCGATTGAGGATAAGTGAGTGCTTACCTGTCCAGCCGAGCCCAGCTTTTTCAGCTAACGGGCGCTCGAGAATAGGCGCAGAAGCGACAAACGGTCTAAAATTCAGCGAAACACAATGTTGCTGAATCATCTCGcc                                                                                             <  1:88263/251‑1 (MQ=255)

                                                                                                                                                        gCAAATCGTCATACAGGCCACGCATTTGCCGCATCCTTCCTCGACTGGTTGATCCAC‑GGGCAGCGGAATATCGACCAGCAATTCGCCTAAAAAAAAGAACGAACCGGCCTCGCGATTGAGGATAAGTGAGTGCTTACCTGTCCAGCCGAGCCCAGCTTTTTCAGCTAACGGGCGCTCGAGAATAGGCGCAGAATCGACAAACGGTCTAAAATTCAGCGAAACACAATGTTGCTGAATCATCTCGCCCAGCt                                                                                        <  2:708217/251‑1 (MQ=255)

                                                                                                                                                         cAAATCGTCATACAGGCCACGCATTTGCCGCATCCTTCCTCGACTGGTTGATCCAC‑GGGCAGCGGAATATCGACCAGCAATTCGCCTAAAAAAAAGAACGAACCGGCCTCGCGATTGAGGATAAGTGAGTGCTTACCTGTCCAGCCGAGCCCAGCTTTTTCAGCTAACGGGCGCTCGAGAATAGGCGCAGAATCGACAAACGGTCTAAAATTCAGCGAAACACAATGTTGCTGAATCATCTCGCCCAGCtt                                                                                       <  1:190000/251‑1 (MQ=255)

                                                                                                                                                          aaaTCGTCATACAGGCCACGCATTTGCCGCATCCTTCCTCGCCTGGTTGATCCAC‑GGGCAGCGGAATATCGACCAGCAATTCGCCTAAAAACAAGAACGAACCGGCCTCGCGATTGAGGCTACGTGAGTGCTTACCTGTCCAGCCGAGCCCAGCTTTTTCAGCTAACGGGCGCTCGAGAATAGGCGCCGAATCGCCAAACGGTCAAAAATTCAGCGAAACACAATGTTGCTGAATCCTCTCGCCCAGCttt                                                                                      >  2:489288/1‑251 (MQ=255)

                                                                                                                                                          aaaTCGTCATACAGGCCACGCATTTGCCGCATCCTTCCTCGACTGGTTGATCCAC‑GGGCAGCGGAATATCGACCAGCAATTCGCCTAAAAAAAAGAACGAACCGGCCTCGCGATTGAGGATAAGTGAGTGCTTACCTGTCCAGCCGAGCCCAGCTTTTTCAGCTAACGGGCGCTCGAGAATAGGCGCAGAATCGACAAACGGTCTAAAATTCAGCGAAACACAATGTTGCTGAATCATCTCGCCCAGCttt                                                                                      >  1:18320/1‑251 (MQ=255)

                                                                                                                                                            aTCGTCATACAGGCCACGCATTTGCCGCATCCTTCCTCGACTGGTTGATCCAC‑GGGCAGCGGAATATCGACCAGCAATTCGCCTAAAAAAAAGAACGAACCGGCCTCGCGATTGAGGATAAGTGAGTGCTTACCTGTCCAGCCGAGCCCAGCTTTTTCAGCTAACGGGCGCTCGAGAATAGGCGCAGAATCGACAAACGGTCTAAAATTCAGCGAAACACAATGTTGCTGAATCATCTCGCCCAGCttttt                                                                                    >  1:754731/1‑251 (MQ=255)

                                                                                                                                                                tCATACAGGCCACGCATTTGCCGCATCCTTCATCGACTGGTTGATCCAC‑GGGCAGCGGAATATAGACAAGCAATTCGCCTAAAAAAAAGAACGAAACGGCATCGCGATTGGGGATAGGTAAGTGATTACACGTCAAGCCGAGACCAGCTTTTTCAGCTAACGGGAGCTCGAGAATAGGCGCAGAATCGCAAAAAGGTATAAAATTACGAGAAACACAATGTTGCTGAATCATCCAGCCCAGCTATTTGatg                                                                                >  2:523852/1‑249 (MQ=255)

                                                                                                                                                                 cctACAGGCCACGCATTTGCCGCATCCTTCCTCGACTGGTTGATCCAC‑GGGCAGCGGAATATCGACCAGCAATTCGCCTAAAAAAAAGAACGAACCGGCCTCGCGATTGAGGATAAGTGAGTGCTTACCTGTCCAGCCGAGCCCAGCTTTTTCAGCTAACGGGCGCTCGAGAATAGGCGCAGAATCGACAAACGGTCTAAAATTCAGCGAAACACAATGTTGCTGAATCATCTCGCCCAGCTTTTTGAGTc                                                                               <  1:753169/249‑1 (MQ=255)

                                                                                                                                                                    aCAGGCCACGCATTTGCCGCATCCTTCCTCGACTGGTTGATCCAC‑GGGCAGCGGAATATCGACCAGCAATTCGCCTAAAAAAAAGAACGAACCGGCCTCGCGATTGAGGATAAGTGAGTGCTTACCTGTCCAGCCGAGCCCAGCTTTTTCAGCTAACGGGCGCTCGAGAATAGGCGCAGAATCGACAAACGGTCTAAAATTCAGCGAAACACAATGTTGCTGAATCATCTCGCCCAGCTTTTTGAGTCGGt                                                                            >  1:577646/1‑251 (MQ=255)

                                                                                                                                                                          cACGCATTTGCCGCATCCTTACTCGACTGGTTGATCCAC‑GGGCAGCGGAATATCGACCAGCAATTCGCCTAAAAAAAAGAACGAACCGGCCTCGCGATTGAGGATAAGTGAGTGCTTACCTGTCCAGCCGAGCCCAGCTTTTTCAGCTAACGGGCGCTCGAGAATAGGCGCAGAATCGACAAACGGTCTAAAATTCAGCGAAACACAATGTTGCTGAATCATCTCGCCCAGCTTTTTGAGTCGGATgcgc                                                                       >  2:944215/1‑250 (MQ=255)

                                                                                                                                                                              cATTTGCCGCATCCTTCCTCGACTGGTTGATCCAC‑GGGCAGCGGAATATCGACCAGCAATTCGCCTAAAAAAAAGAACGAACCGGCCTCGCGATTGAGGATAAGTGAGTGCTTACCTGTCCAGCCGAGCCCAGCTTTTTCAGCTAACGGGCGCTCGAGAATAGGCGCAGAATCGACAAACGGTCTAAAATTCAGCGAAACACAATGTTGCTGAATCATCTCGCCCAGCTTTTTGAGTCGGTTGCGCAGAAg                                                                  <  1:228525/251‑1 (MQ=255)

                                                                                                                                                                               aTTTGCCGCATCCTTCCACGACTGGTTGATCCAC‑GGGCAGCGGAATATCGACCAGCAATTCGCCTAAAAAAAAGAACGAACCGGCCTCGCGATTGAGGATAAGTGATTGCTTACCTGGCCAGCCGAGCCCAGCTTTTTCAGCTAACGGGCGCTCGAGAATAGGCGCAGAATCGACAAACGGTCTAAAATTCAGCGAAACACAATGTTGCTTAAGCATCTCGCCCAGCTTTTTTAGTCGGTTGCGCAGAAGt                                                                 <  1:383499/251‑1 (MQ=255)

                                                                                                                                                                                       cATCCTTCCTCGACTGGTTGATCCAC‑GGGCAGCGGAATATCGACCAGCAATTCGCCTAAAAAAAAGAACGAACCGGCCTCGCGATTGAGGATAAGTGAGTGCTTACCTGTCCAGCCGAGCCCAGCTTTTTCAGCTAACGGGCGCTCGAGAATAGGCGCAGAATCGACAAACGGTCTAAAATTCAGCGAAACACAATGTTGCTGAATCATCTCGCCCAGCTTTTTGAGTCGGTTGCGCAGAAGTTTGTGATa                                                         <  1:778322/251‑1 (MQ=255)

                                                                                                                                                                                          ccttcctCGACTGGTTGATCCAC‑GGGCAGCGGAATATCGACCAGCAATTCGCCTAAAAAAAAGAACGAACCGGCCTCGCGATTGAGGATAAGTGAGTGCTTACCTGTCCAGCCGAGCCCAGCTTTTTCAGCTAACGGGCGCTCGAGAATAGGCGCAGAATCGACAAACGGTCTAAAATTCAGCGAAACACAATGTTGCTGAATCATCTCGCCCAGCTTTTTGAGTCGGTTGCGCAGAAGTTTGTGATAGt                                                       <  1:933548/250‑1 (MQ=255)

                                                                                                                                                                                          acttcctCGGCTGGGTGACCCAC‑GGGCAGCGGAATATCGTCCAGCAATTCGCCTAAAAAAAAGAACGAACCGGCCTCCCGATTGAGGATAAGTGAGTGCTTACCTGTCCAGCCGAGCCCAGCTTTTGCAGCTAACGGGCGCTCGAGAATAGGCGCAGAATCGACAAACGGTCTAAAATTCAGCGAAACACAATGTTGCTGAATCATCTCGCCCAGCTTTTTGAGTCGTTTGCGCAGAAGTGTGTGATAGt                                                       <  2:738800/249‑1 (MQ=255)

                                                                                                                                                                                              cctCGACTGGTTGATCCAC‑GGGCAGCGGAATATCGACCAGCAATTCGCCTAAAAAAAAGAACGAACCGGCCTCGCGATTGAGGATAAGTGAGTGCTTACCTGTCCAGCCGAGCCCAGCTTTTTCAGCTAACGGGCGCTCGAGAATAGGCGCAGAATCGACAAACGGTCTAAAATTCAGCGAAACACAATGTTGCTGAATCATCTCGCCCAGCTTTTTGAGTCGGGTGCGCAGAAGTTTGTGATAGTCACg                                                   <  1:694584/250‑1 (MQ=255)

                                                                                                                                                                                                      ggTTGATCCAC‑GGGCAGCGGAATATCGACCAGCAATTCGCCTAAAAAAAAGAACGAACCGGCCTCGCGATTGAGGATAAGTGAGTGCTTACCTGTCCAGCCGAGCCCAGCTTTTTCAGCTAACGGGCGCTCGAGAATAGGCGCAGAATCGACAAACGGTCTAAAATTCAGCGAAACACAATGTTGCTGAATCATCTCGCCCAGCTTTTTGAGTCGGTTGCGCAGAAGTTTGTGATAGTCACGGCCCAgcg                                           <  1:511011/250‑1 (MQ=255)

                                                                                                                                                                                                         agacccAC‑TGGCAGCGGAATAACGACCAGCAATTCGCTGAAAAAAAAGAACGAACCGGCCGCGCGATTGAGGATAAGTGAGTGCTTACCTGTCCAGCCGAGCCCAGCTTTTTCAGCTAACGGGCGCTCGAGAATAGGCGCAGAATCGACAAACGGTCTAAAATTCAGCGAAACACAATGTTGCTGAATCATCTCGCCCAGCTTTTTGAGTCGGTTGCGCAGAAGTTTGTGATAGTCACGGCCCAGCGCAt                                        <  2:16530/246‑1 (MQ=255)

                                                                                                                                                                                                               aC‑GGGCAGCGGAATATCGACCAGCAATTCGCCTAAAAAAAAGAACGAACCGGCCTCGCGATTGAGGATAAGTGAGTGCTTACCTGTCCAGCCGAGCCCAGCTTTTTCAGCTAACGGGCGCTCGAGAATAGGCGCAGAATCGACAAACGGTCTAAAATTCAGCGAAACACAATGTTGCTGAATCATCTCGCCCAGCTTTTTGAGACGGATGCGCAGAAGTTTGTGATAGTCACGGCCCAGAGCATAAAGGCt                                 >  2:327598/1‑251 (MQ=255)

                                                                                                                                                                                                                    ccAGCGGAATATCGCCCTGCACTTCGACTAAAAAAAAGAACGAACCCGCCTCGCGATTGTGGATAAGTGAGTGCTTACCGGTCCAGCCGAGCCCAGCTTATTCAGCTAACGGGCGCTCGAGAATAGGCGCAGAATCGACAAACGGTCTAAAATTCAGCGAAACACAAGGTTGGTGAATCACCTCGCCCAGCGTTTTGAGTCGGTTGCGCAGAAGGTTGTGATAGTCTCGGCCCAGCGCATAACGGCTAACa                             <  2:389693/250‑1 (MQ=255)

                                                                                                                                                                                                                     cagtggAATATCGGCCAGCAATTAGCCTAAAAAAAAGAACGAACCGGACTCGCGATTGAGAATAAGTGTGTGCTTACCTGTCCAGCAGAGCCCAGATTTTTCAGCTAACGGGCGCTCGCGAATAGGCGCAGAATCGACAAAAGGTCTAAAATTCAGCGAAACACAATGTTGCTGAATCATCTCGCCCCGCTTTTTGAGACGGTTGCTCTGAAGTTTTTGTAAATCACGGACCAGCGCCTAACGGcacccac                            >  2:883654/5‑245 (MQ=255)

                                                                                                                                                                                                                       gCGGAATATCGACCAGCAATTCGCCTAAAAAAAAGAACGAACCGGCCTCGCGATTGAGGATAAGTGAGTGCTTACCTGTCCAGCCGAGCACAGCTTTTTCAGCTAACGGGCGCTCGAGAATAGGCGCAGAATCGACAAACGGTCTAAACTTCAGCGAAACACAATGTTGCTGAATCATCTAGCCCAGCTTTTTGAGGCGGCTGCACAGAAGATTGTGTTAGTCACGGCCAAGCGCATAACGGCTAACAtcg                          >  1:199732/1‑249 (MQ=255)

                                                                                                                                                                                                                        cGGAATATCGACCAGCAATTCGCCTAAAAAAAAGAACGAACCGGCCTCGCGATTGAGGATAAGTGAGTGCTTACCTGTCCAGCCGAGCCCAGCTTTTTCAGCTAACGGGCGCTCGAGAATAGGCGCAGAATCGACAAACGGTCTAAAATTCAGCGAAACACAATGTTGCTGAATCATCTCGCCCAGCTTTTTGAGTCGGTTGCGCAGAAGTTTGTGATAGTCACGGCCCAGCGCATAACGGCTAACATAGc                         <  1:751040/251‑1 (MQ=255)

                                                                                                                                                                                                                         ggAATATCGACCAGCAATTCGCCTAAAAAAAAGAACGAACCGGCCTCGCGATTGAGGATAAGTGAGTGCTTACCTGTCCAGCCGAGCCCAGCTTTTTCAGCTAACGGGCGCTCGAGAATAGGCGCAGAATCGACAAACGGTCTAAAATTCAGCGAAACACAATGTTGCTGAATCATCTCGCCCAGCTTTTTGAGTCGGTTGCGCAGAAGTTTGTGATAGTCACGGCCCAGCGCATAACGGCTAACATAGcc                        <  1:185547/251‑1 (MQ=255)

                                                                                                                                                                                                                          gAATATCGACCATCAAGTCGCCTAAAAAAAAGAACGAACCGGCCTCGCGATTGAGGATAAGTGAGTGCTTACCTGTCCAGCCGAGCCCAGCTTTTTCAGCTAACGGGCGCTCGAGAATAGGCGCAGAATCGACAAACGGTCTAAAATTCAGCGAAACACAATGTTGCTGAATCATCTCGCCCAGCTTTTTGAGTCGGTTGCGCAGAAGTTTGTGATAGTCACGGCCCAGCGCATAACGGCTAACATAGCCg                       <  1:1196/251‑1 (MQ=255)

                                                                                                                                                                                                                                  aCCAGCAATTCGCATAAAAAAAAGAACGACACGGCCTCGCGATTGAGGATAAGTCAGTGCTTACCTGTCCAGCCGCGCCCAGCTTGTTCAGCTAACGGGCGCTCGAGAATAGTCGCATACTCGACAAACGGTCTAAAATTCAGCGAAACACAAGGTTGCTGAATCATCTCGCCCAGCTTTTTGAGTCGGTTGCGCAGAAGTTTGTGATATTCACGGCCCAGCGCATAACGTCTAACATAGCCGAGTTTggg               <  2:465993/251‑1 (MQ=255)

                                                                                                                                                                                                                                       cAATTCGCCTAAAAAAAAGAACGAACCGGCCTCGCGATTGAGGATAAGTGAGTGCTTACCTGTCCAGCCGAGCCCAGCTTTTTCAGCTAACGGGCGCTCGAGAATAGGCGCAGAATCGACAAACGGTCTAAAATTCAGCGAAACACAATGTTGCTGAATCATCTCGCCCAGCTTTTTGAGTCGGTTGCGCAGAAGTTTGTGATAGTCACGGCCCAGCGCATAACGGCTAACATAGCCGAGTTTGGGGtttt          >  1:187093/1‑251 (MQ=255)

                                                                                                                                                                                                                                         aTTCGCCTAAAAAAAAGAACGAACCGGCCTCGCGATTGAGGATAAGTGAGTGCTTACCTGTCCAGCCGAGCCCAGCTTTTTCAGCTAACGGGCGCTCGAGAATAGGCGCAGAATCGACAAACGGTCTAAAATTCAGCGAAACACAATGTTGCTGAATCATCTCGCCCAGCTTTTTGAGTCGGTTGCGCAGAAGTTTGTGATAGTCACGGCCCAGCGCATAACGGCTAACATAGCCGAGTTTGGGGTTTTTc        >  1:851294/1‑251 (MQ=255)

                                                                                                                                                                                                                                           tCGCCTAAAAAAAAGAACGAACCGGCCTCGCGATTGAGGATAAGTGAGTGCTTACCTGTCCAGCCGAGCCCAGCTTTTTCAGCTAACGGGCGCTCGAGAATAGGCGCAGAATCGACAAACGGTCTAAAATTCAGCGAAACACAATGTTGCTGAATCATCTCGCCCAGCTTTTTGAGTCGGTTGCGCAGAAGTTTGTGATAGTCACGGCCCAGCGCATAACGGCTAACATAGCCGGGTTTGGGGtttttt        >  2:297358/1‑248 (MQ=255)

                                                                                                                                                                                                                                              ccTAAAAAAAAGAACGAACCGGCCTCGCTATTGAGGAATAGTGAGTGCTTACTTGTCCAGCCGAGCCCAGCTTTTTCAGCTAACGGGCGCTCGAGAATAGGCGCAGAATCGACAAACGTTCTAAAATTCAGCGAAACACAATGTTGCTGAATCATCTCGCCCAGCTTTTTGAGTCGGTTGCGCAGAAGTTTGTGATAGTCACGGCCCAGCGCATAACGGCTAACATAGCCGAGTTTGGGGTTTTTCAGCGt   <  2:861581/251‑1 (MQ=255)

                                                                                                                                                                                                                                               cTAAAAAAAAGAACGAACCGGCCTCGCGATTGAGGATAAGTGAGTGCTTACCTGTCCAGCCGAGCCCAGCTTTTTCAGCTAACGGGCGCTCGAGAATAGGCGCAGAATCGACAAACGGTCTAAAATTCAGCGAAACACAATGTTGCTGAATCATCTCGCCCAGCTTTTTGAGTCGGTTGCGCAGAAGTTTGTGATAGTCACGGCCCAGCGCATAACGGCTAACACAGCCGGGGTTGGGGTTTTTCAGAGTg  >  1:504252/1‑251 (MQ=255)

GGGCAGATAAGCTGGCAGTCATCGCAACCGTAAATACGGTTTCCCATTAACGGTCGCAACTCTTCCGGGATCGCCCCTTCAAGTTCGATGGTGAGATAAGAGATACAGCGGCGAGCATCGACGGTATATGGCTCGACGATGGCACCGGTCGGGCAAATCGTCATACAGGCCACGCATTTGCCGCATCCTTCCTCGACTGGTTGATCCAC‑GGGCAGCGGAATATCGACCAGCAATTCGCCTAAAAAGAAGAACGAACCGGCCTCGCGATTGAGGATAAGTGAGTGCTTACCTGTCCAGCCGAGCCCAGCTTTTTCAGCTAACGGGCGCTCGAGAATAGGCGCAGAATCGACAAACGGTCTAAAATTCAGCGAAACACAATGTTGCTGAATCATCTCGCCCAGCTTTTTGAGTCGGTTGCGCAGAAGTTTGTGATAGTCACGGCCCAGCGCATAACGGCTAACATAGCCGAGTTTGGGGTTTTTCAGCGTG  >  NC_000913/4393307‑4393795

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`