Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,392,997	G→A	V357V (GTC→GTT)	queG ←	epoxyqueuosine reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,392,997	0	G	A	97.9%	321.4 / ‑5.3	97	V357V (GTC→GTT)	queG	epoxyqueuosine reductase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (56/38);  minor base C (2/0);  total (58/39)
Fisher's exact test for biased strand distribution p-value = 5.17e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

CTGTAATCTTAATTTCAAAGAAACTTCGCACGGTGAATAGTATTTTTTTAACCTATTGATAGATAAGTTAAAAATTAAGATTCCGTTTTGTCGAGTCGATCACTTGACGATTTTATTCGTCTTGAATTGCAATGCGTTTTTATTTTTATTCACAAGCTGTGGATGAATCAGGCGTCACGCGGTAACCCTTTTTCAATCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCACTTCGACTAT‑GCACGCATTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGTGCTCACCTTTACGACTTTCCAGCGCTGTCAAAATCGTTTCATCCCAAGGGGCATTGCCTAATGCTACGGCGATATTACGCAGCCAACGCAGGTGACCAATACGACGAATCGCCGATCCTT‑CCGTGACTTTTAAAAACTTCTCTTCGCTCCAGGCGAATAACTCAATGAGTTCCG  >  NC_000913/4392761‑4393241                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                       cTGTAATCTTAATTTCAAAGAAACTTCGCACGGTGAATAGTATTTTTTTAACCTATTGATAGATAAGTTAAAAATTAAGATTCCGTTTTGTCGAGTCGATCACTTGACGATTTTATTCGTCTTGAATTGCAATGCGTTTTTATTTTTATTCACAAGCTGTGGATGAATCAGGCGTCACGCGGTAACCCTTTTTCAATCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCACTTCAACTAT‑GCACGCAtt                                                                                                                                                                                                                                         >  1:638584/1‑251 (MQ=255)   gTAATCTTAATTTCAAAGAAACTTCGCACGGTGAATAGTATTTTTTTAACCTATTGATAGAGAAGTTAAAAATTAAGATTCCGTTTTGTCGAGTTGATAACTTGACGATTTTATTCGTCTTGAATTGCAATGCGTTTTTATTTTTATTCACAAGCTGTGGATGAATCAGGCGTCACGCGGTAACCCTTTTTCAATCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCACTTCAACTAT‑GCACGCATTTc                                                                                                                                                                                                                                       <  2:224056/251‑1 (MQ=255)     aaTCTTAATTTCAAAGAAACTTCGCACGGTGAATAGTATTTTTTTAACCTATTGATAGATAAGTTAAAAATTAAGATTCCGTTTTGTCGAGTCGATCACTTGACGATTTTATTCGTCTTGAATTGCAATGCGTTTTTATTTTTATTCACAAGCTGTGGATGAATCAGGCGTCACGCGGTAACCCTTTTTCAATCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCACTTCAACTAT‑GCACGCATTTCg                                                                                                                                                                                                                                      <  1:957407/250‑1 (MQ=255)     aaTCCTAATTTCAAAGAAACTTCGCACGGTGAATAGTAGTTTTTTAACCTATTGATAGATAAGTTAAAAAGTAAAAGTCCGTTTTGTCGAGGCGAGCACTTGACGATTTGATTCGTCTTGAATTGCAATGCGTGTTTATTTTTATTCACAAGCTGTGGATGAATCAGGCGTCACGCGGTAACCCTTTTTCAATCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCTCTTCTACTAT‑TCACTCATTTCg                                                                                                                                                                                                                                      <  2:391410/250‑1 (MQ=255)         ttAATTTCAAAGAAACTTCGCACGGTGAATAGTATTTTTTTAACCTATTGATAGATAAGTTAAAAATTAAGCTTCCGTTTTGTCGACTCGCTCACTTGACGATTTTCTTCGTCTTGAATTGCAATGCGTTTTTATTTTTATTCACAAGCTGTGGATGAATCAGGCGTCACGCGGTAACCCTTTTTCAATCCCCCGACCCAGCCGCTGTTTTTTCGGCAATTGCACTTCCACTAT‑GCACGCATTTAGtctct                                                                                                                                                                                                                                 >  1:585022/1‑251 (MQ=255)               ccAAAGAAACTTCGCACGGTGAATAGTATTTTTTTAACCTATTGATAGATAAGTTAAAAATTAAGATTCCGTTTTGTCGAGGCGATCACTTGACGATTTTATTCGTCTTGAATTGCAATGCGTTTTTATTTTTATTCACAAGCTGTGGATGAATCAGGCGTCACGCGGTAGCCCTTTTTCAATCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCACTTCAACTAT‑GCTCGCATTTCGTCTCTCTAttt                                                                                                                                                                                                                           <  1:571197/250‑1 (MQ=255)                   aGAAACTTCGCACGGTGAATAGTATTTTTTTAACCTATTGATAGATAAGTTAAAAATTAAGATTCCGTTTTGTCGAGTCGATCACTTGACGATTTTATTCGTCTTGCATTGCAATGCGTTTTTATTTTTATTCACAAGCTGTGGATGAATCAGGCGTCACGCGGTAACCCTTTTTCAATCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCACTTCAACTAT‑GCACGCATTTCGTCTCTCTATTtgctg                                                                                                                                                                                                                       >  2:592356/1‑251 (MQ=255)                    gAAACTTCGCACGGTGAATAGTATTTTTTTAACCTATTGATAGATAAGTTAAAAATTAAGATTCCGTTTTGTCGAGTCGATCACTTGACGATTTTATTCGTCTTGAATTGCAATGCGTTTTTATTTTTATTCACAAGCTGTGGATGAATCAGGCGTCACGCGGTAACCCTTTTTCAATCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCACTTCAACTAT‑GCACGCATTTCGTCTCTCTATTtgctgc                                                                                                                                                                                                                      <  1:573893/251‑1 (MQ=255)                            ataCGGTGAATCGTATTTTTTTACCCTATTGATAGATAAGATAAAAATTAAGATTACGTTTTGTCGAGTCGATCACTTGAAGATTTTATTCGTCTTGAATTGCAATGCGTTTTTATTTTTATTCACAAGCTGTGGTTGAATCAGGAGTAACGCGGTAACCCTTTTTCAATCACCCGATCCAGACGCTGTTTTTTCGGCAATTGCACTTCAACTAT‑GCACGCATTTCGTCACTCTATTTGCTACCCAATctc                                                                                                                                                                                                              >  2:554945/3‑249 (MQ=255)                             cACGGTGAATAGTATTTTTTTAACCTATTGATAGATAAGTTAAAAATTAAGATTCCGTTTTGTCGAGTCGTTCACTTGACGAATTTATTCGACTTAAATTGCAATGCGTTTTTATTTTTATTCACAAGCTGTGGATGAATCAGGCGTCACGCGGTAACCCTTTTTCAATCACCCGCAACAGACGCTGTTTTTTCGGCAATGGCATTTCAACTAT‑GCACGCATTTCGTCTCTCTATTTGCTGCGCAATCGcc                                                                                                                                                                                                             >  2:697191/1‑251 (MQ=255)                                   gAATAGTATTTTTTTAACCTATTGATAGATAAGTTAAAAATTAAGATTCCGTTTTGTCGAGTCGATCACTTGACGCTTTTATTCGTCTTGAATTGCAATGCGTTTTTATTTTTATTCACAAGCTGTGGATGAATCAGGCGTCACGCGGTAACCCTTTTTCAATCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCACTTCAACTAT‑GCACGCATTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCt                                                                                                                                                                                                       >  1:39217/1‑251 (MQ=255)                                   gAATAGTATTTTTTTAACCTATTGATAGATAAGTTAAAAATTAAGATTCCGTTTTGTCGAGTCGATCACTTGACGATTTTATTCGTCTTGAATTGCAATGCGTTTTTATTTTTATTCACAAGCTGTGGATGAATCAGGCGTCACGCGGTAACCCTTTTTCAATCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCACTTCAACTAT‑GCACGCATTTCGTCTCTCTATTTGCTTCGCAATCGCCCACGCt                                                                                                                                                                                                       >  1:264780/1‑251 (MQ=255)                                         tATTTTTTTAACCTATTGATAGATAAGTTAAAAATTAAGATTCCGTTTTGTCGAGTCGATCACTTGACGATTTTATTCGTCTTGAATTGCAATGCGTTTTTATTTTTATTCACAAGCTGTGGATGAATCAGGCGTCACGCGGAAACCCTTTTTCAATCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCACTTCAACTAT‑GCACGCATTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGc                                                                                                                                                                                                 >  1:549618/1‑251 (MQ=255)                                          aTTTTTTTAACCTATTGATAGATAAGTTAAAAATTAAGATTCCGTTTTGTCGAGTCGATCACTTGACGATTTTATTCGTCTTGAATTGCAATGCGTTTTTATTTTTATTCACAAGCTGTGGATGAATCAGGCGTCACGCGGTAACCCTTTTTCAATCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCACTTCAACTAT‑GCACGCATTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCt                                                                                                                                                                                                >  1:315608/1‑251 (MQ=255)                                                     cTATTGATAGATAAGTTAAAAATTAAGATTCCGTTTTGTCGAGTCGATCACTTGACGATTTTATTCGTCTTGAATTGCAATGCGTTTTTATTTTTATTCACAAGCTGTGGATGAATCAGGCGTCACGCGGTAACCCTTTTTCAATCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCACTTCAACTAT‑GCACGCATTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGt                                                                                                                                                                                     >  1:597417/1‑251 (MQ=255)                                                       aTTGATAGATAAGTTAAAAATTAAGATTCCGTTTTGTCGAGTCGATCACTTGACGATTTTATTCGTCTTGAATTGCAATGCGTTTTTATTTTTATTCACAAGCTGTGGATGAATCAGGCGTCACGCGGTAACCCTTTTTCAATCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCACTTCAACTAT‑GCACGCATTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTgg                                                                                                                                                                                   >  1:809831/1‑251 (MQ=255)                                                               ataAGTTAAAAATTAAGATTCCGTTTTGTCGAGTCGATCACTTGACGATTTTATTCGTCTTGAATTGCAATGCGTTTTTATTTTTATTCACAAGCTGTGGATGAATCAGGCGTCACGCGGTAACCCTTTTTCAATCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCACTTCAACTAT‑GCACGCATTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGTGCTCAc                                                                                                                                                                           >  1:472220/1‑251 (MQ=255)                                                                ataGTTAAAAATTAAGATTCCGTTTTGTCGAGTCGATCACTTGACGATTTTATTCGTCTTGAATTGCAATGCGTTTTTATTTTTATTCACAAGCTGTGGATGAATCAGGCGTCACGCGGTAACCCTTTTTCAATCACCCGAACCAGACGCTGTTTTCTCGGCAATTGCACTTCAACTAT‑GCACGCATTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGTGCTCAcc                                                                                                                                                                          >  1:417592/3‑251 (MQ=255)                                                                    ttAAAAATTAAGATTCCGTTTTGTCGAGTTGATCACTTGACGATTTTATTCGTCTTGAATTGCAATGCGTTTTTATTTTTATTCACAAGCTGTGGATGAATCAGGCGTCACGCGGTAACCCTTTTTCAATCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCACTTCAACTAT‑GCACGCATTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGTGCTCACCttt                                                                                                                                                                       >  1:102676/1‑250 (MQ=255)                                                                     tAAAAATTAAGATTCCGTTTTGTCGAGTCGATCACTTGACGATTTTATTCGTCTTGAATTGCAATGCGTTTTTATTTTTATTCACAAGCTGTGGATGAATCAGGCGTCACGCGGTAACCCTTTTTCAATCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCACTTCAACTAT‑GCACGCATTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGTGCTCACCTTTAc                                                                                                                                                                     >  1:795415/1‑251 (MQ=255)                                                                      aaaaaTTAAGATTCCGTTTTGTCGAGTCGATCACTTGACGATTTTATTCGTCTTGAATTGCAATGCGTTTTTATTTTTATTCACAAGCTGTGGATGAATCAGGCGTCACGCGGTAACCCTTTTTCAATCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCACTTCAACTAT‑GCACGCATTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGTGCTCACCTTTAc                                                                                                                                                                     <  2:348778/250‑1 (MQ=255)                                                                                aTTCCGTTTTGTCGAGTCGATCACTTGACGATTTTATTCGTCTTGAATTGCAATGCGTTTTTATTTTTATTCACAAGCGGTGGATGAATCAGGCGTCACGCGGTAACCCTTTTTCAATCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCACTTCAACTAT‑GCACGCATTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGTGCTCACCTTTACGACTTTCCAgc                                                                                                                                                          <  2:392066/251‑1 (MQ=255)                                                                                  tCCGTTTTGTCGAGTCGATCACTTGACGATTTTATTCGTCTTGAATTGCAATGCGTTTTTATTTTTATTCACAAGCTGTGGATGAATCAGGCGTCACGCGGTAACCCTTTTTCAATAACCCGAACAAGACGCTGTTTTTTCGGCAATTGCACTTCAACTAT‑GCACGCATTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGTGCTCACCTTTACGACTTTCCAgcgc                                                                                                                                                        >  1:947772/1‑251 (MQ=255)                                                                                  gccGTTTTGTCGAGTCGATCACTTGACGATTTTATTCGTCTGGAATTGCAATGCGTTTTTATGTTTATTCACAAGCTGTGGATGAATCAGGCGTCACGCGGTAACCCTTTTTCAATCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCACTTCAACTAT‑GCACGCATTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGTGCTCACCTTTACGACTTTCCAgcg                                                                                                                                                         <  2:821250/249‑1 (MQ=255)                                                                                    cGTTTTGGCGAGTCGATCACTTGACGATTTTATTCGTCTTGAATTGCAATGCGTTTTTATTTTTATTCACAAGCTGTGGATGAATCAGGCGTCACGCGGTAACCCTTTTTCAATCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCACTTCAACTAT‑GCACGCAGTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGTGCTCACCTTTACGACTTTCCAGCGCTg                                                                                                                                                      <  2:800/251‑1 (MQ=255)                                                                                       tttggctagtctattcttggACGATTTTATTCGTCTTGAATTGCAATGCGGTTTTATTTTTATTCACAAGCTGTGGATGAATCAGGCGTCACGCGGTAACCCTGTTTCAATCACCCGAACCAGACGCTGTGTTTTCGGCAATTGCACTTCAACTAT‑GCACTCATTTCGTCTCTCTATTTGCTGCGCAATCGCCCACTCTATGGGCTTATCGAGAAGTGGGTGCGCACCTTTACGACTTTCCAGCGCTGTc                                                                                                                                                    <  2:910162/231‑1 (MQ=255)                                                                                         tGTCGAGTCGATCACTTGACGATTTTATTCGTCTTGAATTGCAATGCGTTTTTCTTTTTATTCACAAGCTGTGGATGAATCAGGCGTCACGCGGTAACCCTTTTTCAATCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCACTTCAACTAT‑GCACGCATTTCGTCTCTCTATTTGCTGCGCAATCGCCAACGCTATGTGCTCATCGAGAAGTGGTGGCTCACCTTTACGACTTTCCAGCGCTGTCaa                                                                                                                                                  >  2:274130/1‑250 (MQ=255)                                                                                             gagtcgaTCACTTGACGATTTTATTCGTCTTGAATTGCAATGAGTTTTTATTTTTATTCACAAGCTGTGGATGAATCAGGCGTCACGCGGTAACCCTTTTTCAATCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCACTTCAACTAT‑GCACGCATTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGTGCTCACCTTTACGACTTTCCAGCGCTGTCACAATCg                                                                                                                                             >  1:317998/1‑251 (MQ=255)                                                                                               gtcgaTCATATGACGATTTTATACGTCTTGAATTGCAAGGAGTTTTTATTTTTATTCACAAGCTGTGGATGAATCAGGCGTCACGCGGTACATCTTTTTCAATCACCCGAATCAGACGCTGTTTTTTCGGCAATTGCTCTTCAACTAT‑GCACGCATTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGTGCTCACCTTTACGACTTTCCAGCGCTGTCAAAATCGtt                                                                                                                                           <  2:282789/251‑1 (MQ=255)                                                                                                    tCACTTGACGATTTTATTCGTCTTGAATTGCAATGCGTTTTTATTTTTATTCACAAGCTGTGGATGAATCAGGCGTCACGCGGTAACCCTTTTTCAATCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCACTTCAACTAT‑GCACGCATTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGTGCTCACCTTTACGACTTTCCAGCGCTGTCAAAATCGTTTCATc                                                                                                                                      >  1:433891/1‑251 (MQ=255)                                                                                                     cccTTGACGATTTTATTCGTCTTGAATTGCAATGCGTTTTTATTTTTATTCACAAGCTGTGGATGAATCAGGCGTCACGCGGTAACCCTTTTTCAATCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCACTTCAACTAT‑GCACGCATTTCGTCTCTCTATTTGCTTCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGTGCTCACCTTTACGACTTTCCAGCGCTGTCAAAATCGTTTCATcc                                                                                                                                     <  2:264780/249‑1 (MQ=255)                                                                                                     cACTTGACGATTTTATGCGTCTTGAAGTGCAATGCGTTTTTATTTTTATTCACAAGCTGTGTATGAATCAGGCGTCACGCGGTAACCCTGTTTCAATCACCCGAACCAGTCGCTGTTTTTTCGGCAATTGCACTTCAACTAT‑GCACGCATTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGTGCTCACCTGTACGACTTTCCAGCGCTGTCAAAATCGTTTCATcc                                                                                                                                     <  2:39217/251‑1 (MQ=255)                                                                                                      aCTTGACGATTTTATTCGTCTTGAAGTGCAATGCGGTTTTATTTTTATTCACAAGCGTTGGATGAATCAGGCTTCACGCGGTAACCCTTTTTCAATCACCCGAACCAGACGCTGTTTGTTCGGCAATTGCACTTCAACTAT‑GCACGCATTTCGGCTCTCTATTTGCTGCGCAATCGCCCGCGCTATGTGCTCATCGAGAAGTGGGTGCTCACCGTTACGACTTTCCAGCGCTGTCAAAATCGTTTCAtacc                                                                                                                                    <  2:898677/251‑4 (MQ=255)                                                                                                       cTTGACGATTTTATTCGTCTTGAATTGCAATGCGTTTTTATTTTTATTCACAAGCTGTGGATGAATCAGGCGTCACGCGGTAACCCTTTTTCAATCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCACTTCAACTAT‑GCACGCATTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGTGCTCACCTTTACGACTTTCCAGCGCTGTCAAAATCGTTTCATccc                                                                                                                                    >  2:690486/1‑250 (MQ=255)                                                                                                          gACGATTTTATTCGTCTTGAATTGCAATGCGTTTTTATTTTTATTCACAAGCTGTGGATGAATCAGGCGTCACGCGGTAACCCTTTTTCAATCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCACTTCAACTAT‑GCACGCATTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGTGCTCACCTTTACGACTTTCCAGCGCTGGCAAAATCGTTTCATCCCAAg                                                                                                                                 <  2:271253/250‑1 (MQ=255)                                                                                                          gACGATGTTATTCGTCTTGAATTGCAATGCGTTTTTATTGTTATTCACAAGCTGTGGATGAAGCATGCGTCACGCGGTAACCCTTTTTCAATCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCACTTCAACTAT‑GCACGCATTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGTGCTCACCTTTACGACTTTCCAGCGCTGTCAAAATCGTTTCATCCCAAg                                                                                                                                 <  2:310849/250‑1 (MQ=255)                                                                                                            cGATTTTATTCGTCTTGAATTGCAATGCGTTTTTATTTTTATTCACAAGCGGTGGATGAATCAGGCGTCACGCGGTAACCCTTTTTCAATCACCCGAACCAGACGCTTTTTTTTCGGCAATTGCACTTCAACTAT‑GCACGCATTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGTGCTCACCTTTACGACTTTCCAGCGCTGTCAAAATCGTTTCACCCCAAgggg                                                                                                                              <  2:728966/251‑1 (MQ=255)                                                                                                             gATTTTATTCGTCTTGAATTGCAATGCGTTTTTATTTTTATTCACAAGCTGTGGATGAATCAGGCGTCACGCGGTAACCCTTTTTCAATCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCACTTCAACTAT‑GCACGCATTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGTGCTCACCTTTACGACTTTCCAGCGCTGTCAAAATCGTTTCATCCCAAGGGGc                                                                                                                             <  1:28693/251‑1 (MQ=255)                                                                                                                   aTTCGGCTTGAATTGCAATGCGTTTTTATTTTTATTCACAAGCTGTGGAAGAATCAGGCGTCACGCGGTAACCCTTTTTCAATCACCCGAACCAGTCGCTGTTTTTTCGGCAATTGCACTTCAACTAT‑GCACGCATTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGTGCTCACCTTTACGACTTTCCAGCGCTGTCAAAATCGTTTCATCCCAAGGGGCATTGcc                                                                                                                       <  2:708765/251‑1 (MQ=255)                                                                                                                    ttCGTCTTGAATTGCAATGCGTTTTTATTTTTATTCACAAGCTGTGGATGAATCAGGCGTCACGCGGTAACCCTTTTTCAATCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCACTTCAACTAT‑GCACGCATTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGTGCTCACCTTTACGACTTTCCAGCGCTGTCAAAATCGTTTCATCCCAAGGGGCATTGCCt                                                                                                                      >  1:891233/1‑251 (MQ=255)                                                                                                                    ttCGTCTTGAATTGAAATGCGTTTTTATTTTTATTCACAAGCTGTGGATGAATCAGGCGTCACGCGGTAACCCTTTTTCAATCACCCGAACCAGACGCTGTTTTTTAGGCAATTGCACTTCAACTAT‑ACACGCATTTCGTCTCTCTCTTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGGGGGTGCTCACCTTTACGACTTTCCAGCGCTGTCAAAGTCGTATCATCCCACGGGTCATTTCCt                                                                                                                      >  2:904029/1‑251 (MQ=255)                                                                                                                         cTTGAATTGCAATGCGTTTTTATTTTTATTCACAAGCTGTGGATGCATCAGGCGTCACGCGGTAACCCTTTTTCAATCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCACTTCAACTAT‑GCACGCATTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGCGAAGTGGGTGCTCACCTTTACGACTTTCCCGCGCTGTCAAAATCGTTTCATCCCAAGGGGCATTGCCTAATGc                                                                                                                 >  2:387145/1‑251 (MQ=255)                                                                                                                             aaTTGCAATGCGTTTTTATTTTTATTCACAAGCTGTGGATGAATCAGGCGTCACGCGGTAACCCTTTTTCAATCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCACTTCAACTAT‑GCACGCATTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGTGCTCACCTTTACGACTTTCCAGCGCTGTCAAAATCGTTTCATCCCAAGGGGCATTGCCTAATGCTACg                                                                                                             >  1:757587/1‑251 (MQ=255)                                                                                                                              aTTGCAATGCTGTTTTATTTTTATTCACATGCTGTGGATGAATCCGGCGTCACGCGGTATCCCTTTTTCAATCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCACTTCAACTAT‑GCACGCATTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGTGCTCACCTTTACGACTTTCCAGCGCTGTCAAAATCGTTTCATCCCAAGGGGCATTGCCTAATGCTACgg                                                                                                            <  1:521983/251‑1 (MQ=255)                                                                                                                               ttGCAATGCGTTTTTATTTTTATTCACAAGCTGTGGATGAATCAGGCGTCACGCGGTAACCCTTTTTCAATCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCACTTCAACTAT‑GCACGCATTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGTGCTCACCTTTACGACTTTCCAGCGCTGTCAAAATCGTTTCATCCCAAGGGGCATTGCCTAATGCTACGGc                                                                                                           >  1:131918/1‑251 (MQ=255)                                                                                                                                ttcAATGCGTGTTTTTTGTTAGTCACAAGCTGTGGATGAATCAGGCTACACGCGGTTCCCCTTTTTTAATGACCCGAACCATACGCTGGTTTTTCGGCAATTGCACTCAAACGAT‑GCACGCATTTCGTCTGTCGATTTGGTTCGCAATCGCCCACGCTATGTGCTCATCGTGAAGTTGGTGCTCACCTTTACGGCTTTCCAGTGCTGGCAAAATCGTTTCATCCCAAGGGGCATTGCCTCATGCTACGGc                                                                                                           <  2:882991/248‑1 (MQ=255)                                                                                                                                tGCAATGCGTTTTTATTTTTATTCACAAGCTGTGGATGAATCAGGCTTCACTCGGTAACCCTTTTTCAATCACCCGAACCATACGCTGTTTTTTCGGCAATTGCACTTCAACTAT‑GCACGCATTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGTGCTCACCTTTACGACTTTCCAGCGCTGTCAAAATCGTTTCATCCCAAGGGGCATTGCCTAATGCTACGGc                                                                                                           <  2:433891/250‑1 (MQ=255)                                                                                                                                tGCAATGCGTTTTTATTTTTATTCACAAGCTGTGGATGAATCAGGCGTCACGCGGTAACCCTTTTTCAATCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCACTTCAACTAT‑GCACGCATTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGTGCTCACCTTTACGACTTTCCAGCGCTGTCAAAATCGTTTCATCCCAAGGGGCATTGCCTAATGCTACGGc                                                                                                           <  1:398932/250‑1 (MQ=255)                                                                                                                                   aaTGCGTTTTTATTTTTATTCACAAGCTGTGGATGAATCAGGCGTCACGCGGTAACCCTTTTTCAATCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCACTTCAACTAT‑GCACGCATTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGTGCTCACCTTTACGACTTTCCAGCGCTGTCAAAATCGTTTCATCCCAAGGGGCATTGCCTAATGCTACGGCGat                                                                                                        >  1:424980/1‑250 (MQ=255)                                                                                                                                    aTGCGTTTTTATTTTTATTCACAAGCTGTGGATGAATCAGGCGTCACGCGGTAACCCTTTTTCAATCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCACTTCAACTAT‑GCACGCATTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGTGCTCACCTTTACGACTTTCCAGCGCTGTCAAAATCGTTTCATCCCAAGGGGCATTGCCTAATGCTACGGCGatat                                                                                                      <  1:194680/251‑1 (MQ=255)                                                                                                                                       cGTTTTTATTTTTATTCACAAGCTGTGGATGAATCAGGCGTCACGCGGTAACCCTTTTTCAATCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCACTTCAACTAT‑GCACGCATTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGTGCTCACCTTTACGACTTTCCAGCGCTGTCAAAATCGTTTCATCCCAAGGGGCAt                                                                                                                           <  1:215561/227‑1 (MQ=255)                                                                                                                                       cGTTTTTATTTTTATTCACAAGCTGTGGATGAATCAGGCGTCACGCGGTAACCCTTTTTCAATCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCACTTCAACTAT‑GCACGCATTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGTGCTCACCTTTACGACTTTCCAGCGCTGTCAAAATCGTTTCATCCCAAGGGGCAt                                                                                                                           >  2:215561/1‑227 (MQ=255)                                                                                                                                         tttttatttttaTTCACAAGCTGTGGATGAATCAGGCGTCACGCGGTAACCCTTTTTCAATCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCACTTCAACTAT‑GCACGCATTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGACGTGGGTGCTCACCTTTACGACTTTCCAGCGCTGTCAACATCGTTTCATCCCAAGCGGCATTGCCTAAGCCTACGGcactattaagc                                                                                                 >  2:228525/1‑241 (MQ=255)                                                                                                                                             tatttttattCACAAGCTGTGGATGAATCAGGCGTCACGCGGTAACCCTTTTTCAATCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCACTTCAACTAT‑GCACGCATTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGTGCTCACCTTTACGACTTTCCAGCGCTGTCAAAATCGTTTCATCCCAAGGGGCATTGCCTAATGCTACGGCGATATTACGCAGcc                                                                                             >  2:504140/1‑251 (MQ=255)                                                                                                                                              atttttattCACAAGCTGTGGATGAATCAGGCGTCACGCGGTAACCCTTTTTCAATCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCACTTCAACTAT‑GCACGCATTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGTGCTCACCTTTACGACTTTCCAGCGCTGTCAAAATCGTTTCATCCCAAGGGGCATTGCCTAATGCTACGGCGATATTACGCAGcc                                                                                             <  1:172364/250‑1 (MQ=255)                                                                                                                                                ttttattCACAAGCTGTGGATGAATCAGGCGTCACGCGGTAACCCTTTTTCAATCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCACTTCAACTAT‑GCACGCATTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGTGCTCACCTTTACGACTTTCCAGCGCTGTCAAAATCGTTTCATCCCAAGGGGCATTGCCTAATGCTACGGCGATATTACGCAGCCAAc                                                                                          >  1:788575/1‑251 (MQ=255)                                                                                                                                                 tttattCACAAGCTGTGGATGAATCAGGCGTCACGCGGTAACCCTTTTTCAATCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCACTTCAACTAT‑GCACGCATTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGTGCTCACCTTTACGACTTTCCAGCGCTGTCAAAATCGTTTCATCCCAAGGGGCATTGCCTAATGCTACGGCGATATTACGCAGCCAACg                                                                                         >  1:605127/1‑251 (MQ=255)                                                                                                                                                  tttttgacatgatgtgGAAGAATCAGGCGTCACGCGGTAACCATTTTTCACTCACCCGAACAAGACGCTATTTTTTCGGCACTTGCACTTCAACTAT‑GCTCGCATTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGACGCAGGTGATCACCTTTACGACTCTCCAGCGCTGTCAAAATCGTTTCATCCCCAGGGGCATTGCCTAGTGCTACGGCGATAttcagccgccaactc                                                                                        >  2:623659/13‑238 (MQ=255)                                                                                                                                                  ttattCACAAGCTGTGGATGAATCAGGCGTCACGCGGTAACCCTTTTTCAATCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCACTTCAACTAT‑GCACGCATTTCG‑‑TCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGTGCTCACCTTTACGACTTTCCAGCGCTGTCAAAATCGTTTCATCCCACGGGGCATTGCCTAATGCTACGGCGATATTACGCAGCCAACGc                                                                                        >  1:530274/1‑249 (MQ=255)                                                                                                                                                   tattCACAAGCTGTGGCTAAATCAGGCATCACGCGGTAACCCTTTTTCAAGCACCCGCACCAGACGCTGTTTTTTCGGCAATTGCACTTCCACTATACCAC‑CCTTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCCATGTGATCATCGAGAAGGTGGAGCTCTCCTTTACGACTTTCCCGATCTGTCAAATTCGTTTAATCCCCAGGAGAATTGCCTAAAGATACGGTGATATTTCGCAGACAAcac                                                                                        >  2:686611/1‑248 (MQ=255)                                                                                                                                                    attCACAAGCTGTGGATGAATCAGGCGTCACGCGGTAACCCTTTTTCACTCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCACTTCAACTAT‑GCACGCATTTCGTCTATCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGTGCTCACCTTTACGACTTTCCAGCGCTGTCAAAATCGTTTCATCCCACGGGGCATTGCCTAATGCTACGGCGATATTACGCAGCCACCGc                                                                                        >  1:416216/1‑249 (MQ=255)                                                                                                                                                     ttCACAAGCTGTGGATGAATCAGGCGTCACGCGGTAACCCTTTTTCAATCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCACTTCAACTAT‑GCACGCATTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGTGCTCACCTTTACGACTTTCCAGCGCTGTCAAAATCGTTTCATCCCAAGGGGCATTGCCTAATGCTACGGCGATATTACGCAGCCAACGCAgg                                                                                     >  1:710688/1‑251 (MQ=255)                                                                                                                                                        acaAGCTGTGGATGAATCAGGCGTCACGCGGTAACCCTTTTTCAATCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCACTTCAACTAT‑GCACGCATTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGTGCTCACCTTTACGACTTTCCAGCGCTGTCAAAATCGTTTCATCCCAAGGGGCATTGCCTAATGCTACGGCGATATTACGCAGCCAACGCAGGTGa                                                                                  <  1:497799/251‑1 (MQ=255)                                                                                                                                                              tgtgGATGAATCAGGCGTCACGCGGTAACCCTTTTTCAATCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCACTTCAACTAT‑GCACGCATTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGTGCTCACCTTTACGACTTTCCAGCGCTGTCAAAATCGTTTCATCCCAAGGGGCATTGCCTAATGCTACGGCGATATTACGCAGCCAACGCAGGTGACCAAt                                                                             >  1:204262/1‑250 (MQ=255)                                                                                                                                                               gtgGATGAATCAGGCGTCACGCGGTAACCCTTTTTCAATCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCACTTCAACTAT‑GCACGCATTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGTGCTCACCTTTACGACTTTCCAGCGCTGTCAAAATCGTTTCATCCCAAGGGGCATTGCCTAATGCTACGGCGATATTACGCAGCCACCGCAGGTGACCAATac                                                                           >  1:368662/1‑251 (MQ=255)                                                                                                                                                                     gAATCAGGCGTCACGCGGTAACCCTTTTTCAATCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCACTTCAACTAT‑GCACGCATTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGTGCTCACCTTTACGACTTTCCAGCGCTGTCAAAATCGTTTCATCCCAAGGGGCATTGCCTAATGCTACGGCGATATTACGCAGCCAACGCAGGTGACCAATAcgacga                                                                      >  1:42655/1‑250 (MQ=255)                                                                                                                                                                     gAATCAGGCGTCACGCGGTAACCCTTTTTCAATCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCACTTCAACTAT‑GCACGCATTTCGTCTCTCTATTTGCTGCGCAATCGCC‑ACGCTATGTGCTCATCGAGAAGTGGGTGCTCACCTTTACGACTTTCCAGCGCTGTCAAAATCGTTTCATCCCAAGGGGCATTGCCTAATGCTACGGCGATATTACGCAGCCAACGCAGGTGACCAATACGACGAAt                                                                    >  1:465993/1‑251 (MQ=255)                                                                                                                                                                         cAGGCGTCACGCGGTACCCCTTTTTCAATCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCACTTCAAATAT‑GCACGAATTTAGTATCTATATTTGCTGCGCAATCGCCCCCGATATGTGCTCATCTAGAAGTGGGTGCTCACCTTTACGACTTTCCAGAGCTGTCACAATGGTTTCATCCCAAGGGGCATTGCCTAATGCTACGGAGATATTACGCAGCCACAGCAGGTGACCAATAAGACGAAtagc                                                                 >  2:63696/1‑248 (MQ=255)                                                                                                                                                                                cACGCGGTAACCCTTTTTCAATCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCACTTCAACTAT‑GCACGCATTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGTGCTCACCTTTACGACTTTCCAGCGCTGTCAAAATCGTTTCATCCCAAGGGGCATTGCCTAATGCTACGGCGATATTACGCAGCCAACGCAGGTGACCAATACGACGAATCGCCGAttct                                                          <  1:389531/251‑4 (MQ=255)                                                                                                                                                                                     ggTAACCCTTTTTCAATCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCACTTCAACTAT‑GCACGCATTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCAGCGAGAAGTGGGTGCTCACCTTTACGACTTTCCAGCGCTGTCAAAATCGTTTCATCCCAAGGGGCATTGCCTAATGCTACGGCGATATTACGCAGCCAACGCAGGTGACCAATACGACGAATCGCCGATCCTT‑CCGt                                                    <  1:58608/251‑1 (MQ=255)                                                                                                                                                                                      gTAACCCTTTTTCAATCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCACTTCAACTAT‑GCACGCATTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGTGCTCACCTTTACGACTTTCCAGCGCTGTCAAAATCGTTTCATCCCAAGGGGCATTGCCTAATGCTACGGCGATATTACGCAGCCAACGCAGGTGACCAATACGACGCATCGCCGATCCTT‑CCGTg                                                   >  1:676282/1‑251 (MQ=255)                                                                                                                                                                                       tAACCCTTTTTCAATCACCCGAACCAGAAGCTGTTTTTTCGGCAATTGCACTTCAACTAT‑GCACGCATTTCGTCTCTCTATTTGCTGCGCACTCGCCCACGCTATGTGCTCATCGAGAAGTGGGTGCTCACCTTTACGACTTTCCAGCGCTGTCAAAATCGTTTCATCCCAAGGGGCATTGCCTAATGCTACGGCGATATTACGCAGCCAACGCAGGGGACCAATACGACGAATCGCCGATCCTT‑CCGTg                                                   >  2:600742/1‑250 (MQ=255)                                                                                                                                                                                          cccTTTTTCCATCAACCGAACCAGACGCTGTTTTTTCGGCAATTGCACTTCAACTAT‑GCACGCATTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGCGAAGTGGGTGCTCACCTTTACGACTTTCCAGCGCTGTCAAAATCGTTTCATCCCACGGGGCCTTGCCTAATGCTACGGCGATCTTACGCAGCCAACGCAGGTGACCAATACGACGAATCGCCGACACTT‑CCGTGACtt                                               >  2:449574/1‑251 (MQ=255)                                                                                                                                                                                           ccTTTTTCAATCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCACTTCAACTAT‑GCACGCATTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGTGCTCACCTTTACGACTTTCCAGCGCTGTCAAAATCGTTTCATCCCAAGGGGCATTGCCTAATGCTACGGCGATATTACGCAGCCAACGCAGGTGACCAATACGACGAATCGCCGATCCTT‑CCGTGACttt                                              <  1:271449/251‑1 (MQ=255)                                                                                                                                                                                              ctttCAAGCACCCTATCCAGATGTTGTCTTTACGGTAATTGAACTTCAACTAT‑GCTCGCATTTCGTCTCTCTATTTGCTGCTCAATCGCCCACGCTAAGTGCTCATCGATAAGTTGGTGCTCACCTTTACGACTTTCCAGCGCGGTCAAAATCGTTTCATCCCAAGTGGCATTGCCTAATGCTACGTCGATATTACGCAGCCAACGCAGGTGACCAATTCGACGAATCGCCGATCCTT‑CCGTGACtttt                                             <  2:724662/248‑1 (MQ=255)                                                                                                                                                                                                  cAATCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCACTTCAACTAT‑GCACGCATTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGTGCTCACCTTTACGACTTTCCAGCGCTGTCAAAATCGTTTCATCCCAAGGGGCATTGCCTAATGCTACGGCGATATTACGCAGCCAACGCAGGTGACCAATACGACGAATCGCCGATCCTT‑CCGTGACTTTTAAAAAc                                       >  2:239349/1‑251 (MQ=255)                                                                                                                                                                                                      cACCCGAACCAGACGCTGTTTTTTCGGCAATTGCACTTCAACTAT‑GCACGCATTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGTGCTCACCTTTACGACTTTCCAGCGCTGTCAAAATCGTTTCATCCCAAGGGGCATTGCCTAATGCTACGGCGATATTACGCAGCCAACGCAGGTGACCAATACGACGAATCGCCGATCCTT‑CCGTGACTTTTAAAAACTtct                                   >  1:408574/1‑251 (MQ=255)                                                                                                                                                                                                       aCCCGAACCAGACGCTGTTTTTTCGGCAATTGCACTTCAACTAT‑GCACGCATTTCGTCTCTCTATTTGATGCGCAATAGCCCACGCTATGTGCTCATCGAGAAGTGGGTGCTCACCTTTACGACTTTCCAGCGCTGTCAAAATCGTTTCATCCCAAGGGGCATTGCCTAATGCTACGGCGATATTACGCAGCCAACGCAGGAGACCAATACGACGAATCGCCGATCCTT‑CAGTGACTTTTAAAAACTtctc                                  >  1:671698/1‑251 (MQ=255)                                                                                                                                                                                                          cGAACCAGACGCTGTTTTTTCGGCAATTGCACTTCAACTAT‑GCACGCATTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGTGCTCACCTTTACGACTTTCCAGCGCTGTCAAAATCGTTTCATCCCAAGGGGCATTGCCTAATGCTACGGCGATATTACGCAGCCAACGCAGGTGACCAATACGACGAATCGCCGATCCTT‑CCGTGACTTTTAAAAACTTCTCTTc                               <  1:690486/251‑1 (MQ=255)                                                                                                                                                                                                           gAACCAGTCGCTGTTTTTTCGGCAAGTGCACTTCAACTAT‑GCACGCATTTCGTTTCTCTATTTGCTGCGCAATCGCCCACGCTAGGTGCGCATCGAGaa                                                                                                                                                                                       <  2:976868/99‑1 (MQ=255)                                                                                                                                                                                                           gAACCAGACGCTGTTTTTTCGGCAATTGCACTTCAACTAT‑GCACGCATTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGaa                                                                                                                                                                                       >  1:976868/1‑99 (MQ=255)                                                                                                                                                                                                              ccAGACGCTGTTTTTTCGGCAATTGCACTTCAACTAT‑GCACGCATTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGTGCTCACCTTTACGACTTTCCAGCGCTGTCAAAATCGTTTCATCCCAAGGGGCATTGCCTAATGCTACGGCGATATTACGCAGCCAACGCAGGTGACCAATACGACGAATCGCCGATCCTT‑CCGTGACTTTTAAAAACTTCTCTTCGCTc                           <  2:235322/251‑1 (MQ=255)                                                                                                                                                                                                               cAGACGCTGTTTTTTCGGCAATTGCACTTCAACTAT‑GCACGCATTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGTGCTCACCTTTACGACTTTCCAGCGCTGTCAAAATCGTTTCATCCCAAGGGGCATTGCCTAATGCTACGGCGATATTACGCAGCCAACGCAGGTGACCAATACGACGAATCGCCGATCCTT‑CCGTGACTTTTAAAAACTTCTCTTCGCTcc                          >  1:607489/1‑251 (MQ=255)                                                                                                                                                                                                                     cTGTTTTTTCGGCAATTGCACTTCAACTAT‑GCACGCATTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGTGCTCACCTTTACGACTTTCCAGCGCTGTCAAAATCGTTTCATCCCAAGGGGCATTGCCTAATGCTACGGCGATATTACGCAGCCAACGCAGGTGACCAATACGACGAATCGCCGATCCTT‑CCGTGACTTTTAAAAACTTCTCTTCGCTCCAGGCg                     >  1:670472/1‑250 (MQ=255)                                                                                                                                                                                                                        ttttttCGGCAATTGCACTTCAACTAT‑GCACGCATTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGTGCTCACCTTTACGACTTTCCAGCGCTGTCAAAATCGTTTCATCCCAAGGGGCATTGCCTAATGCTACGGCGATATTACGCAGCCAACGCAGGTGACCAATACGACGAATCGCCGATCCTT‑CCGTGACTTTTAAAAACTTCTCTTCGCTCCAGGCGAAt                  >  1:389693/1‑250 (MQ=255)                                                                                                                                                                                                                         tttttCGGCAATTGCACTTCAACTAT‑GCACGCATTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGTGCTCACCTTTACGACTTTCCAGCGCTGTCAAAATCGTTTCATCCCAAGGGGCATTGCCTAATGCTACGGCGATATTACGCAGCCAACGCAGGTGACCAATACGACGAATCGCCGATCCTT‑CCGTGACTTTTACAAACTTCTCTTCGCTCCAGGCGAATa                 >  2:677106/1‑250 (MQ=255)                                                                                                                                                                                                                         tttttCGGCAATTGCACTTCAACTAT‑GCACGCATTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGTGCTCACCTTTACGACTTTCCAGCGCTGTCAAAATCGTTTCATCCCAAGGGGCATTGCCTAATGCTACGGCGATATTACGCAGCCAACGCAGGTGACCAATACGACGAATCGCCGATCCTT‑CCGTGACTTTTAAAAACTTCTCTTCGCTCCAGGCGAATa                 >  2:198616/1‑250 (MQ=255)                                                                                                                                                                                                                             tCGGCAATTGCACTTCAACTAT‑GCACGCATTTCGTCTCTCTATTTGCTGCGCACTCGCCCACGCTATGTGCTCATCGCGAAGTGGGTGCTCACCTTTACGACTTTCCAGCGCTGTCAAAATCGTTTCATCCCAAGGGGCATTGCCTAATGCTACGGCGATATTACGCAGCCAACGCAGGTGACCAATACGACGAATCGCCGCTCCTT‑CCGTGACTTTTAAAACCTTCTCTTCGCTCCAGGCGAATACCTc             >  2:347189/1‑250 (MQ=255)                                                                                                                                                                                                                             tCGGCAATTGCACTTCAACTAT‑GCACGCATTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGTGCTCACCTTTACGACTTTCCAGCGCTGTCAAAATCGTTTCATCCCAAGGGGCATTGCCTAAGCCTCCGGCGTTATTACACGGCAAACGCAGGTGCCCAATCAGACGACTCGCCG‑TCCTTTCCGTAATTTTTAAAACCTTCTCTTccatacggccgaataattc             >  2:347174/1‑232 (MQ=255)                                                                                                                                                                                                                              cGGCAATTGCACTTCAACTAT‑GCACGCATTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGTGCTCACCTTTACGACTTTCCAGCGCTGTCAAAATCGTTTCATCCCAAGGGGCATTGCCTAATGCTACGGCGATATTACGCAGCCAACGCAGGTGACCAATACGCCGAATCGCCGATCCTT‑CCGTGCCTTTTAAAAACTTCTCTTCGCTCCAGGCGAATAACTCa            >  2:359398/1‑250 (MQ=255)                                                                                                                                                                                                                              cGGCAATTGCACTTCAACTAT‑GCACGCATTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGTGCTCACCTTTACGACTTTCCAGCGCTGTCAAAATCGTTTCATCCCAAGGGGCATTGCCTAATGCTACGGCGATATTACGCAGCCAACGCAGGTGACCAATACGACGAATCGCCGATCCTT‑CCGTGACTTTTAAAAACTTCTCTTCGCTCCAGGCGAATAACTCa            >  1:827693/1‑250 (MQ=255)                                                                                                                                                                                                                              cGGCAATTGCACCGCAACTAT‑GCCCGCATTTCGTCTCTCTAGTTGCTGCCCAATCGCCCACGCGATTGGCGCATCGAGAAGTGGGTGATCACCTTTACGACATTCCAGCGTTGTCAAAATCGGTTCATCCCAAGGGGCATTGCCTAATGCTACGGCGATATTACTCAGCCAACGCAGGTGACCAATACGACGAATCGCCGATCCTT‑CCGTGACTTTGAAAAACTGCTCTTCGCTCCAGGCGAATAACTCaa           <  2:585022/251‑1 (MQ=255)                                                                                                                                                                                                                                 cAATTGCACTTCAACTAT‑GCACGCATTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGTGCTCACCTTTACGACTTTCCAGCGCTGTCAAAATCGTTTCATCCCAAGGGGCATTGCCTACTGCTACGGCGATATTACGCAGCCAACGCAGGTGACCAATACGACGAATCGCCGATCCTT‑CCGTGACTTTTAAAAACTTCTCTCCGCTCCAGGCGAATAACTCAATg         >  2:19181/1‑250 (MQ=255)                                                                                                                                                                                                                                  aaTTGCACTTCAACTAT‑GCACGCATTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGTGCTCACCTTTACGACTTTCCAGCGCTGTCAAAATCGTTTCATCCCAAGGGGCATTGCCTAATGCTACGGCGATATTACGCAGCCAACGCAGGTGACCAATACGACGAATCGCCGATCCTT‑CCGTGACTTTTAAAAACTTCTCTTCGCTCCAGGCGAATAACTCAATGAg       <  1:285676/251‑1 (MQ=255)                                                                                                                                                                                                                                     tGCACTTCAACTAT‑GCACGCATTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGTGCTCACCTTTACGACTTTCCAGCGCTGTCAAAATCGTTTCATCCCAAGGGGCATTGCCTAATGCTACGGCGATATTACGCAGCCAACGCAGGTGACCAATACGACGAATCGCCGATCCTT‑CCGTGACTTTTAAAAACTTCTCTTCGCTCCAGGCGAATAACTCAATGAGtt     <  1:673297/250‑1 (MQ=255)                                                                                                                                                                                                                                       cACTTCAACTAT‑GCACGCATTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGTGCTCACCTTTACGACTTTCCAGCGCTGTCAAAATCGTTTCATCCCAAGGGGCATTGCCTAATGCTACGGCGATATTACGCAGCCAACGCAGGTGACCAATACGACGAATCGCCGATCCTT‑CCGTGACTTTTTAAAACTTCTCTTCGCTCCAGGCGAATAACTCAATGAGTTCCg  <  2:417592/251‑1 (MQ=255)                                                                                                                                                                                                                                       cACTTCAACGAT‑GCCCGCATTTCGTCGCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGTGCTCACCGTTACGACTTTCCAGCGCTGTCAAAATCGTTTCATCCCAAGGGGCATTGCCTAATGCTACGGCGATATTACGCAGCCAACGCAGGTGACCAATACGACGAATCGCCGATCCTT‑CCGTGACTTTTAAAAACTTCTCTTCGCTCCAGGCGAATAACTCAATGAGTTCCg  <  2:472220/251‑1 (MQ=255)                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                       CTGTAATCTTAATTTCAAAGAAACTTCGCACGGTGAATAGTATTTTTTTAACCTATTGATAGATAAGTTAAAAATTAAGATTCCGTTTTGTCGAGTCGATCACTTGACGATTTTATTCGTCTTGAATTGCAATGCGTTTTTATTTTTATTCACAAGCTGTGGATGAATCAGGCGTCACGCGGTAACCCTTTTTCAATCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCACTTCGACTAT‑GCACGCATTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGTGCTCACCTTTACGACTTTCCAGCGCTGTCAAAATCGTTTCATCCCAAGGGGCATTGCCTAATGCTACGGCGATATTACGCAGCCAACGCAGGTGACCAATACGACGAATCGCCGATCCTT‑CCGTGACTTTTAAAAACTTCTCTTCGCTCCAGGCGAATAACTCAATGAGTTCCG  >  NC_000913/4392761‑4393241

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑