Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,223,638	T→C	intergenic (‑10/‑190)	iclR ← / → metH	DNA‑binding transcriptional repressor IclR/cobalamin‑dependent methionine synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,223,638	0	T	C	100.0%	307.9 / NA	91	intergenic (‑10/‑190)	iclR/metH	DNA‑binding transcriptional repressor IclR/cobalamin‑dependent methionine synthase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (23/68);  total (23/68)

TCGCCAACCTGACGCACGAAACCCTGCTGTTGCATCGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAA‑TCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTC‑CAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCG‑TTTCGCGGGAATGGGTGCGACCATGACAGTCTCTTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATG‑AGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTG  >  NC_000913/4223396‑4223884                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                        tCGCCCACCGGACGCACGAAACCCTGCTGTTGCATCGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAA‑TCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTC‑CAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCG‑TTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCt                                                                                                                                                                                                                                                  <  2:282643/250‑1 (MQ=255)  cGCCAACCTGACGCACGAAACCCTGCTGTTGCATCGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAA‑TCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTC‑CAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCG‑TTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGt                                                                                                                                                                                                                                                <  1:247615/251‑1 (MQ=255)  cGCCAACCTGACGCACGAAACCCTGCTGTTGCATCGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAA‑TCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTC‑CAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCG‑TTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGt                                                                                                                                                                                                                                                <  1:46430/251‑1 (MQ=255)     cAACCTGACGCACGAAACCCTGCTGTTGCATCGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAA‑TCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTC‑CAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCG‑TTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATc                                                                                                                                                                                                                                             <  2:501430/251‑1 (MQ=255)      aaCCTGACGCACGAAACCCTGCTGTGGCATCGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAA‑TCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTC‑CAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCG‑TTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCg                                                                                                                                                                                                                                            <  2:178648/251‑1 (MQ=255)        ccTGACGCACGAAACCCTGCTGTTGCATCGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAA‑TCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTC‑CAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCG‑TTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTg                                                                                                                                                                                                                                          <  1:308747/251‑1 (MQ=255)          ggACGCGCGAAACCCTGCTTTGGCATCGTGGTTAGATGGCGGTGGGTCGTGGAAGTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAA‑TCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTC‑CAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCG‑TTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTTTATCGttga                                                                                                                                                                                                                                        <  2:233945/250‑4 (MQ=255)           gACGCACGAAACCCTGCTGTTGCATCGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAA‑TCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTC‑CAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCG‑TTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGaa                                                                                                                                                                                                                                       <  1:743580/251‑1 (MQ=255)             cGCACGAAACCCTGCTTTTGCATCGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCTGCTTGGTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAA‑TCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTC‑CAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCG‑TTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAAt                                                                                                                                                                                                                                     <  2:343430/251‑1 (MQ=255)               cACGAAACCCTGCTGTTGCATCGTGGTTAGCAGGCGGTGGGGCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAA‑TCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTC‑CAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCG‑TTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAAtca                                                                                                                                                                                                                                   <  2:789606/251‑1 (MQ=255)                ccgacaCCCTGCTGTTGCAGCGTGGTTTGCAGGCGGTGGGTCGTGGAATTGGGTAACCCTGCTTGTTGCGCCAGTTCCGTGAGTGCCGCACGGCCATTGGATTCGGCAA‑TCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTC‑CAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCG‑TTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAAtcat                                                                                                                                                                                                                                  <  2:798055/246‑1 (MQ=255)                 cGAAACCCTGCTGTTGCATCGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAA‑TCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTA‑CAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCG‑TTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAAtcatt                                                                                                                                                                                                                                 <  1:374418/251‑1 (MQ=255)                      cgctgcgggtGCATCGTGGTTAGAAGGCGGTGTGTCGTGGAATGGGGCAACCCTGATTGTTGCGCCTGTTCCGTGAGTGCCACTCTGCCATTGGATTCGTCCA‑TCCACTCCAGTAAGTTCAGGCCACGCGTTAAAGACTGAACCTGTC‑CAGTCGCTGGTGCGGTTGGATCGGCGGGTTTTCTGCCGCG‑TTTCGCGGGAATGGGTGCGACCAGGACAGTCTCCTTTTTCTGTATCGTGGAAAtcattttcat                                                                                                                                                                                                                            <  2:106065/242‑1 (MQ=255)                       ccTGCTGTTGCATCGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAA‑TCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTC‑CAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCG‑TTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAAtcattttcatt                                                                                                                                                                                                                           <  2:209930/251‑1 (MQ=255)                          gctgTTGCATCGTGGTTAGCAGGCGGTGGTGCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAA‑TCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTC‑CAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCG‑TTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAAtcattttcattt                                                                                                                                                                                                                          <  1:581553/249‑1 (MQ=255)                             gTTGCATCGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCGTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAA‑TCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTC‑CAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCT‑TTTCGCGGGAATGGGTGCGACCATCTCAGTCTCCTTTTTCTGTATctttgaaagaagtgaaaggtttatt                                                                                                                                                                                                                     <  1:72668/251‑25 (MQ=255)                               tGCATCGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAA‑TCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTC‑CAGTCGCTGGTGCGGTGGCAACGGCGGGGTTTCTTCCGCG‑TTTCGCGGGGAAGGGTGCGGACATGGCAGTCTCCTTTTTTTTTATCTTGGGAATCATTTTTATTTTTTTTGt                                                                                                                                                                                                                   >  1:893822/1‑251 (MQ=255)                                     gTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAA‑TCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTC‑CAGTCGCTGGTGCGGTGGCAACGGCGGGGTTTCTGCCGGG‑GGTCGGGGGGAAGGGGGGGACCAAGGCAGACTCCTTTTTTTGTATTGTGGAAATTATTTTCCttttttttttttactt                                                                                                                                                                                                             >  1:540245/1‑240 (MQ=255)                                       ggTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACACCGCTTGTTGCGCCAGTTACGAGAGTGCCACACTGCCATTGGATTCGGTAA‑TCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAATCTGTC‑CAGTCTCTGGTGCGTTGGCCACGGCGGGTTTTCGGCTTCG‑TTTCGCGGGAATGGGTGCTATCATCGCAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAAt                                                                                                                                                                                                           <  1:886483/251‑1 (MQ=255)                                       ggTTAGCAGGCGGTGGGTCGTGGAATGGGGTAACCCTGCTTGTAGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAA‑TCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTC‑CAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCG‑TTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAAt                                                                                                                                                                                                           <  2:244629/251‑1 (MQ=255)                                          tAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAA‑TCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTC‑CAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCG‑TTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGc                                                                                                                                                                                                         <  2:840045/250‑1 (MQ=255)                                                gttggggggcgcggAATTGGGTAACCCTGTTCGTCGCGCCAGTGCCTGGAGTGCCACATTGCCATTGGATTCGGCAA‑TCCACTCCAGGAATTTCAGGCCAAGCGGTAAAGACTGAACCTGTC‑CAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCG‑TTTCGCTGTAATGGTTGCGACCATTACAGTCTCCTTTTTCTGTATCGTGGAAATCAATTTCATTTTTATTGTTAGCTAATGCAATAGtt                                                                                                                                                                                                  <  2:179136/239‑1 (MQ=255)                                                gCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAA‑TCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTC‑CAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCG‑TTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGtt                                                                                                                                                                                                  <  1:734871/251‑1 (MQ=255)                                                   gTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAA‑TCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTC‑CAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCG‑TTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTact                                                                                                                                                                                               <  1:364728/251‑1 (MQ=255)                                                    ggggTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAA‑TCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTC‑CAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCG‑TTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTactg                                                                                                                                                                                              <  1:895611/250‑1 (MQ=255)                                                     gggTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAA‑TCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTC‑CAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCG‑TTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTactga                                                                                                                                                                                             <  1:1575/251‑1 (MQ=255)                                                          gTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAA‑TCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTC‑CAGTCGCTGGTGCGGTGGCAACGGCGGGGTTTCTTGCGCG‑TTTCTCGGGAAAGGGTGCGGCCATGAAAGGCTCCCTTTTTTGTTTCGTGGAAATCAATTTCATTTTTATTTTTTTCTAATGCCAAAGTTactgaaatg                                                                                                                                                                                         >  1:861330/1‑247 (MQ=255)                                                            ggAATTTGGTAACCCGGCTTGTGGCGCCAGTTCCGTGAGTTCCACACTGCCATTGGATTCGGCAA‑TCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTC‑CAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCG‑TTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGAt                                                                                                                                                                                       <  1:321581/250‑1 (MQ=255)                                                            ggAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAA‑TCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTC‑CAGTCGCTGGTGCGGTGGCAACGGCGGGGTTTTTGCCGCG‑TTTCGCGGGGATGGGTGGGACCCAGACAGTCTCCCTTTTTTTTATCGGGGGAATCCTTTTTATTTTTATTTTTAGATAATGCAATAATTACTTAAATGATc                                                                                                                                                                                      >  1:869890/1‑251 (MQ=255)                                                             gAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAA‑TCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTC‑CAGTCGCTGGTGCGGTGGCAACGGCGGGGTTTTTGCCGCGGTTTC‑CGGGGATGGGGGGGGCCACGACAGTCTCCTTTTTTTGTATTGTGGAAAACCTTTTCATTTTTTTTTTTTGCTAATGCAATAGTTACTGGACTGAttc                                                                                                                                                                                     >  1:595865/1‑249 (MQ=255)                                                             gAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAA‑TCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTC‑CAGTCGCTGGTGCGGTGGCAACGGAGGGTTTTCTGCCGCG‑TTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATcc                                                                                                                                                                                     <  1:354868/251‑1 (MQ=255)                                                                 tGGGTAACTCTGCTTGTTGCGCCAGATCCGTGAGTGCCACACTGCCATGTGATTCGGCAA‑TCCACTCCAGGAATTTCAGGCCACGCGTTAAAGACTGAACCTGTA‑CAGTCGCTGGTGCGGTGGCAACGGCGGTTTTTCTGCCGCG‑TTTCGCGGGAATGGGTTCGACCATGACAGTCTCCTTCTTCTGTATCGTGGAAATCATTTGCATGTTTATTGTTAGTTAATGAAATAGTTactgaactgaccctat                                                                                                                                                                                  <  2:991308/250‑7 (MQ=255)                                                                   ggTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAA‑TCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTC‑CAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCG‑TTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATG‑Ag                                                                                                                                                                              <  1:702218/251‑1 (MQ=255)                                                                       aCCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAA‑TCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTC‑CAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCG‑TTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATG‑AGTTaa                                                                                                                                                                          <  1:697716/251‑1 (MQ=255)                                                                           ggCTTGTTGCTCCCGTTCCGTGGATGCCCTACTGGCATTTGATTCGGCAA‑TCCTCTCTAGTAATGTCAGGCCACGCGTTAAAGACGGATCCTGGC‑CAGTCGCTGGTTCGGTGGCAACGGCGTGTTTTCTGTCTCG‑TTTCGCGTGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTTGAAATCATTTTCATTTTTATTGATAGCTAACGCAATAGTTACTGAACTGATCCGATG‑AGTTAAtttt                                                                                                                                                                      <  2:561575/251‑4 (MQ=255)                                                                               tgttgCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAA‑TCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTC‑CAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCG‑TTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATG‑AGTTAATGTTGaa                                                                                                                                                                   <  1:635801/250‑1 (MQ=255)                                                                               gtttgCGCCAGTGCCGTGAGTGCCACACTGCCATTGGATTCGGCAA‑TCCCCTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTC‑CAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCG‑TTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATG‑AGTTAATGTTGAAc                                                                                                                                                                  <  2:165888/249‑1 (MQ=255)                                                                                  tgCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAA‑TCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTC‑CAGTCGCTGGTGCGGTGGCAACGGCGGGGTTTTTGCCGCG‑TTTCTCGGGAATGGGGGCGGCCATGGAAGGCTCCTTTTTTTGTTTCGTGGGAATCATTTTCTTTTTTTTTGTTAGCTCATTCAAATGTTTCTGGACTTATCCG‑TGTAGTTTATGTTGAACaa                                                                                                                                                                >  1:42783/1‑250 (MQ=255)                                                                                      ccAGTTCCGTGAGGGCCACACGGCCATTGGATTCGGCAA‑TCCACTCCAGTAAGTTAAGGCCACGCGTTAAAGACTGAACCTGTA‑CAGTCGCTGGTGCGGGGGCATCGGCGGGTTTTCTGCCGCG‑TTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATG‑AGTTAATGTTGAACAAAtctc                                                                                                                                                           <  2:883187/251‑1 (MQ=255)                                                                                      ccAGTTCCGTACGTGCCACACTTCCATTGGATTCCGCAAGTCC‑CTCCAGTAATGTCAGGCCACGCGTTAAAGACTTAACCTGTC‑CCGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCG‑TTTCGCGGGAATGTGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATG‑AGTTAATGTTGAACAAAtctc                                                                                                                                                           <  2:540023/251‑1 (MQ=255)                                                                                             cGTGAGTGCCACCCTGCCATTGGTTGCGGCAA‑TGCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTC‑CAGGCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCG‑TTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATG‑AGTTAATGTTGAACAAATCTCATGTTGc                                                                                                                                                    <  2:180283/251‑1 (MQ=255)                                                                                              gTGAGTGCCACACTGCCATTGGATTCGGCAA‑TCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTC‑CAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCG‑TTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATG‑AGGTAATGTTGAACAAATCTCATGTTGCg                                                                                                                                                   <  1:868165/251‑1 (MQ=255)                                                                                                gAGTGCCACACTGCCATTGGATTCGGCAA‑TCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTC‑CAGTCGCTGGTGCGGTGGCAACGGCGGGGTTTTTGCCGCG‑GTTCGCTGGAATGGGGGCGGCCATGACCGTCTCCTTTTTTTGTATCGTGGAAATCATTTTCATTTTTTTTGTTTTCTTATGCCATAGTTTCTGAACTGATCCCATG‑AGAGAATGTTGTACAAATCTCCTGTTGcttg                                                                                                                                                 >  1:832335/1‑248 (MQ=255)                                                                                                      cccacTGCCATTGGAGTCGGCAA‑TCCACTCCAGTAAGTGCAAGCCACGCGTTAAAGACGGAACCTGTC‑CAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCG‑TGTCGCGGGAATGGGTGCTACCATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATACGATG‑AGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTc                                                                                                                                           <  2:574717/249‑1 (MQ=255)                                                                                                            gCCATTGGATTCGGCAA‑TCCACTCCAGTAATTTCATGCCACGCGTTAAAGACTGAACCTGTC‑CAGGCGCTGGTGCGGTGGCAACGGCGTGTTTTCTGCCGCG‑TTTCGCGGGAATTGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTTGAAATCAGTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACGGATCCGATG‑AGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCtttt                                                                                                                                     <  2:861330/251‑1 (MQ=255)                                                                                                                      tctgCAA‑GCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTC‑CAGTCGCTGGTGCGGTGGCAACTGCGGGTTTTCTGCCGCG‑TTGCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCGGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTTATGCAATAGTTACTGAACTGATCCGATG‑AGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGAt                                                                                                                            <  2:913293/247‑1 (MQ=255)                                                                                                                       cGGCAA‑TCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTC‑CAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCG‑TTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATG‑AGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATg                                                                                                                           <  1:428086/250‑1 (MQ=255)                                                                                                                        ggCAA‑TCCACTCCTGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTC‑CAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCG‑TTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTTGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATG‑AGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCTCTTTTACCACAGATGCg                                                                                                                         <  2:441572/251‑1 (MQ=255)                                                                                                                        ggCAA‑TCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTC‑CAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCG‑TTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATG‑AGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCg                                                                                                                         <  1:679239/251‑1 (MQ=255)                                                                                                                           catctcACTCCAGTAATTTCTGGTCACGCGTTAAAGTGTGAACC‑GTCTCAGTCGCTGGTGCGTTGTCAACGGCTGGTTTTCTGCCGCG‑TTTCGCGGGTATGTGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATG‑AGTTAATGTTGAACAAATCTCAGGTTGCGTGGTGTTCGCTTTTACCACAGACGCGtt                                                                                                                       <  2:642837/246‑1 (MQ=255)                                                                                                                             tacacctcctctAATTTCAGGCCACGCGTTAAAGACTGATCCTGTC‑CAGTCTCCGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCG‑TTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTTGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTTCTGAACTGATCCGATG‑AGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTCCCACAGATGCGttt                                                                                                                      <  2:175386/239‑1 (MQ=255)                                                                                                                               ccACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTC‑CAGTCGCTGGTGCGGTGGCAACGGCGGGGTTTCTGCCGCG‑TTTTGCGGGGATGGGGGCGACCATGACAGTCTCCTTTTTTTGTATCGTGGGAATCATTTTCATTTTTTTTTTTTGCTTATGCAATAGTTACTGAACTGATCCGATG‑AGTTAATGTTTAACAAATCTCATGTTTCGTGGTGGTCGCTTTTACCACAGATGCGTTTAtt                                                                                                                   >  1:506605/1‑250 (MQ=255)                                                                                                                                         aaTTTCAGGCCACGCGTTAAAGACTGAACCTGTC‑CAGTCGCGGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCG‑TTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATG‑AGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATgg                                                                                                          <  2:105337/250‑1 (MQ=255)                                                                                                                                               aGGCCTCGCGGTAAAGACTGACCCGGTC‑CAGTCGCGGGTTCGGTGGCATCGTCGGGTTTTCTGCCGCG‑TTTCGCGGGAATGGGTGCGTCCATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTGTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATG‑AGTTAATGGTGAACAAAGCTCATGTTGCGTGGTGGTCGCTTCTACCACAGATGCTTTTATGCCAGGATGGTttgttg                                                                                                   <  2:993039/251‑1 (MQ=255)                                                                                                                                               aGGCCACGCGTTAAAGACTGAACCTGTC‑CAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCG‑TTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATG‑AGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTttgttg                                                                                                   <  1:553550/251‑1 (MQ=255)                                                                                                                                               aGGCCACGCGTTAAAGACTGAACCTGTC‑CAATCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCG‑TTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATG‑AGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTttgttg                                                                                                   <  2:119958/251‑1 (MQ=255)                                                                                                                                                     cgcgTTAAAGACTGAACCTGTC‑CAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCG‑TTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATG‑AGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAAtttt                                                                                             <  1:972737/251‑1 (MQ=255)                                                                                                                                                        gTTAAAGACTGAACCTGTC‑CAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCG‑TTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTAt                                                                                                                                                                                                                      <  1:10941/128‑1 (MQ=255)                                                                                                                                                           aaaGGCTGAACCGGGC‑CAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCG‑TTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATG‑AGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTaa                                                                                       <  2:540811/251‑1 (MQ=255)                                                                                                                                                           aaaGACTGAACCTGTC‑CAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCG‑TTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATG‑AGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTttatta                                                                                        <  2:170057/250‑1 (MQ=255)                                                                                                                                                            aaGACTGAACCTGTC‑CAGTCGCTGGTGCGGTGGCAACGGCGGGGTTTCTGCCGCG‑GTTCGCGGGAATGGGGGCGACCCTGAAAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTTTTGTTATCTAATGCAAAAGTTACTGAACTGATCCGATG‑TGGTTATGTTTAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATTCCCGTATGGTTTGTTGGAtttttttttt                                                                                       >  1:862078/1‑245 (MQ=255)                                                                                                                                                             aGACTGAACCTGTC‑CAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCG‑TTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATG‑AGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAAt                                                                                     <  1:368613/251‑1 (MQ=255)                                                                                                                                                              gACTGAACCTGTC‑CATGCGCTGGTGCGGTGGCAACGGCGTGTTTTCGGCCGCG‑TTTCGCGGGAATGGTTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATG‑AGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGTTTGTACCACAGATGCGTTTATGCCAGTATGGTTTGTTTAATTTTTATTAAATc                                                                                    <  2:747767/251‑1 (MQ=255)                                                                                                                                                                        gTC‑CAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTTTGCCGCG‑TTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATG‑AGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGCTGAATTTTTATTAAATCTGGGTTGAg                                                                           <  2:706425/250‑1 (MQ=255)                                                                                                                                                                               gcGCTGGTGCTGTGGCCACGGCGGGTTTTCTGCCGCG‑TTTCGGGGGATTGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAATTATTTTCATTTTTATTGTTAGCTAATGCAATAGTTTCTGAACTGTTCCGATG‑AGTTAATGTTGTACAAATCTCATGTTGCGAGGTGGTCTCGTTTTCCACAGAGGCTTTTATGCCAGTATGGTTTGTTGAATTTTTATTATCTCGGGGTTGAGTGTGTct                                                                    <  2:590364/250‑2 (MQ=255)                                                                                                                                                                                        cGGTGGCTACGGCGGGTTTGCTGCCGCG‑TTTCGCGTGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGGATCGTGGAAATCATTTTCATTTGTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATG‑AGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCGTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAgt                                                           <  1:530698/251‑1 (MQ=255)                                                                                                                                                                                              cagggtcggGTTTTCTGTCGCG‑TTTCGCTGGAATGGGTGCGACCATGATAGTCTCCTTTTTCTGTATGGTGGAAATCATTTTCTTTTTTATTGTTAGCTAATGCAATAGTTTCTGAATTGATCCGATG‑AGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTGTACCACAGATGCGTTTATGCCAGGATGTTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGagc                                                     <  2:977224/245‑1 (MQ=255)                                                                                                                                                                                                  ggcggGTTTTCTGCCGCG‑TTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATG‑AGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGagcagc                                                  >  1:503606/1‑250 (MQ=255)                                                                                                                                                                                                     gggTTTTCTGCCGCG‑TTTCGCGGGAATGGGTGCGACCATGCCAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGATAATGCAATAGTTACTGAACTGATCCGATG‑AGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCACGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCATGTGTGAGCAGCaa                                                >  1:969471/1‑249 (MQ=255)                                                                                                                                                                                                         tttCTGCCGCG‑TTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCTATAGTTACTGAACTGATCCGATG‑AGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGTGGCAAGTGTGAGCAGCCAAGTgg                                           >  2:261564/1‑250 (MQ=255)                                                                                                                                                                                                          tgcTGCCGCG‑TTTCGCGGGAATGGGTGCGACTATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATG‑AGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGTCAGGATGGTTTGTTGAATTTTTCTTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGa                                          <  1:413360/248‑1 (MQ=255)                                                                                                                                                                                                           tCTGCCGCG‑TTTCGCGGTAATGGGTGCGACTATGACAGTTTCCTTTTTCTGTAACGTGGAAATCATTTTCATTTTTATTGTTAGCTAATTCAATAGTTACTGAACTGATCCGATG‑AGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCTTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGaa                                         <  2:519926/250‑1 (MQ=255)                                                                                                                                                                                                           tCTGCCGCG‑TTTCGCGGGAATGGGTGCGACCATGACAGGCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATG‑AGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGaa                                         <  2:506605/250‑1 (MQ=255)                                                                                                                                                                                                            cTGCCGCG‑TTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATG‑AGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGaac                                        >  2:84051/1‑250 (MQ=255)                                                                                                                                                                                                               ccGCG‑TTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATGTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATG‑AGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACt                                    <  1:820809/251‑1 (MQ=255)                                                                                                                                                                                                                cgcg‑TTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATG‑AGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAAttt                                                                                              <  2:951761/192‑1 (MQ=255)                                                                                                                                                                                                                   g‑TTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATG‑AGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACtgcgt                                >  1:337198/1‑251 (MQ=255)                                                                                                                                                                                                                          cgGGAATTGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATG‑AGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCGGTGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAg                          <  1:499358/251‑1 (MQ=255)                                                                                                                                                                                                                          cgGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATG‑AGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTgcgc                            >  1:979892/1‑249 (MQ=255)                                                                                                                                                                                                                           gggAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTCTCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCACTAGTTACTGAACTGATCCGATG‑AGTTACTGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACAACAGATGCGTTAATGCCAGTCTGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGAGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGTAAAAACTACGTGCGCAGt                         >  2:560278/1‑251 (MQ=255)                                                                                                                                                                                                                           gggAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATG‑AGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGt                         <  1:940590/251‑1 (MQ=255)                                                                                                                                                                                                                              aaTGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATG‑AGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTaa                      <  1:73400/251‑1 (MQ=255)                                                                                                                                                                                                                               aTGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAATCTTTTTAATTTTTATTGTTAGCTAATGCAATCGTTACTAAGCTGATCCGATG‑AGTTAATGTTGGACAAATCTCATGTTGCGAGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGATTTTTTATTAAATCTGGGTTGAGCGGGTCGGGAGCCAGTGAGAGTAGCAAGGTGGAACAACTGCGTGCGCAGGTaat                     >  2:282358/1‑250 (MQ=255)                                                                                                                                                                                                                               aTGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGCACTGATCCTATG‑AGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGACAGTGTGAGCAGAAAAGTGTAACAACGGCGTGCGCAGTTaa                      >  2:61135/1‑250 (MQ=255)                                                                                                                                                                                                                               aTGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATG‑AGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTaa                      >  2:166277/1‑250 (MQ=255)                                                                                                                                                                                                                                            tGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATG‑AGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGAGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTg        >  2:511655/1‑251 (MQ=255)                                                                                                                                                                                                                                             gtcAGTCTCCTTTTTCTTTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATG‑ATTTAATGTTGAACAAATCTCATGTTGCGTTGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTAGTCTgg       <  2:479666/249‑1 (MQ=255)                                                                                                                                                                                                                                             gACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGAGCCGATG‑AGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTGTACCACGGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTg                                                                                  <  1:558564/176‑1 (MQ=255)                                                                                                                                                                                                                                               cAGTCTCCTTTTTCTGTATCGTGGAAATCATCTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATG‑AGTTAATGTTGAACAAATCTCATGTTGCATGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGCGCGTGTAGGGAGCAAGTGTGAGCAGCAAAGTGGCACACCTGCGTGCGCAGTTAAATGAActaatttgggtg     >  2:980870/1‑240 (MQ=255)                                                                                                                                                                                                                                                 gTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATG‑AGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCt   >  1:901328/1‑251 (MQ=255)                                                                                                                                                                                                                                                  tctcCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTACTGCAATAGATACTGAACTGATCCGATG‑AGTTAATGTTGAACAAATCTCATGTTGCGTGGAGGTCGCTTTTACCACAGATGCGTATATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGTGCGTGTCGTGAGCCAGTGTGAGCAGCAAGGTGGAACAACTGCGTGCGCAGTTACATGAACGTCTTCTGGAGCTg  >  1:631028/1‑251 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                        TCGCCAACCTGACGCACGAAACCCTGCTGTTGCATCGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAA‑TCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTC‑CAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCG‑TTTCGCGGGAATGGGTGCGACCATGACAGTCTCTTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATG‑AGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTG  >  NC_000913/4223396‑4223884

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑