Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,215,347	T→A	intergenic (+138/‑131)	metA → / → aceB	homoserine O‑succinyltransferase/malate synthase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,215,347	0	T	A	100.0%	170.8 / NA	50	intergenic (+138/‑131)	metA/aceB	homoserine O‑succinyltransferase/malate synthase A
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (21/29);  total (21/29)

GCGAGCTGGCGTAGTCACGGTAATTTACTGTTTACCAACTGGCTCAACTATTACG‑TCTACCAGATCACGCCATACGATCTACGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCGGGTGCCTTTTTTATTTCCGAAACGTACCTCAGCAGGTGAATAAATTTTATTCATATTGTTATCAACAAGTTATCAAGTATTTTTAATTAAAATGGAAATTGTTTTTGATTTTGCATTTTAAATGAGTAGTCTTAGTTGTGCTGAACGAAAAGAGCACAACGATCCTTCGTTCACAGTGGGGAAGTTTTCGGATCCATGACGAGGAGCTGCACGATGACTGAACAGGCAACAACAACCGATGAACTGGCTTTCACAAGGCCGTATGGCGAGCAGGAGAAGCAAATTCTTACTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTG  >  NC_000913/4215102‑4215588                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                  gcgatcTGGCGTCGTCAAGGTAATTTAGTGTTTACCAACTGGCTCAACTATTTCG‑TCTACCAGATCACGCCATACGATCTAAGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCTATTCAGCACCTTACCTCAGGCACCTTCGGGTGCTTTTTTTATTTTCGAAACGTACCTCAGCAGGTGAATAAATTTTATTCATATTGTTATCAACAAGTTATCAAGTATTTataatt                                                                                                                                                                                                                                              <  2:653234/251‑1 (MQ=255)        ggCGTAGTCACGGTAATTTACTGTTTACCAACTGGCTCAACTATTACG‑TCTACCAGATCACGCCATACGATCTACGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCGGGTGCCTTTTTTATTTCCGAAACGTACCTCAGCAGGTGAATAAATTTTATTCATATTGTTATCAACAAGTTATCAAGTATTTATAATTAAAAt                                                                                                                                                                                                                                         <  2:418301/249‑1 (MQ=255)                  cGGTAATTTACTGTTTACCAACTGGCTCAACTATTACG‑TCTACCAGATCACGCCATACGATCTACGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCGGGTGCCTTTTTTATTTCCGAAACGTACCTCAGCAGGTGAATAAATTTTATTCATATTGTTATCAACAAGTTATCAAGTATTTATAATTAAAATGGAAATTGtttt                                                                                                                                                                                                                             >  1:491010/1‑251 (MQ=255)                      aaTTTATTGTTTACCAACTGGCTCAACTATTACG‑TCTACCAGAACACGCCATACGAACTACGGCACATTAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCGGGTTCCTTTTTTATTTCCGAAACGTACCTCAGCAGGTGAATAAATTTTATTCATATTGTTATCAACAAGTTATCAAGTATTTATAATTAAAATGGAAATTGTTTTTGAt                                                                                                                                                                                                                         <  2:249500/251‑1 (MQ=255)                       aTTTACTGTTTACCAACTGGCTCAACTATTACG‑TCTACCAGATCACGCCATACGATCTACGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCGGGTGCCTTTTTTATTTCCGAAACGTACCTCAGCAGGTGAATAAATTTTATTCATATTGTTATCAACAAGTTATCAAGTATTTATAATTAAAATGGAATTTGTTTTTgttt                                                                                                                                                                                                                        >  2:272800/1‑248 (MQ=255)                           aCTGTATACCAACTTGCACAACTATTACG‑TCTACCAGCTCACGCCATACGATCAATGACACATGAATCCAACGCTGGATTAATATTATGTGATAGTCGATCGTTACGTGCTTCAACACCTTACCTCAGGCACCTTCGGGTGCCTTTTTTATTTCCGAAACGTCCCTCAGCAGGTGAAAAAATTTTATTCCTATTGTTATCAACCAGTTATCAGATATTTATAATTAAAATGGAAATTATTTTTGATTTTGc                                                                                                                                                                                                                    >  2:903711/1‑251 (MQ=255)                               tttACCAACTGGCTCAACTATTACG‑TCTACCAGATCACGCCATACGATCTACGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCGGGTGCCTTTTTTATTTCCGAAACGTACCTCAGCAGGTGAATAAATTTTATTCATATTGTTATCAACAAGTTATCAAGTATTTATAATTAAAATGGAAATTGTTTTTGATTTTGCAttt                                                                                                                                                                                                                >  1:433056/1‑251 (MQ=255)                                 tACCAACTGGCTCAACTAGTTCGATC‑ACCAGATCACGCCATACGATCTACTGCACATGAATCCATCGCTGGATTAATCTTCTTTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCGGGTTCCTTTTTTATTTCCGAAACGTACCTCAGCATGTGAATAAATTTTATTCATATTGTTATCAACAAGTTATCAAGTATTTATAATTAAAATGGAAATTGTTTTTGATTTTGCAtttt                                                                                                                                                                                                               <  2:341006/250‑1 (MQ=255)                                    cAACTGGCTCAACTATTACG‑TCTACCAGATCACGCCATACGATCTACTGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTAAGCACCTTACCTCAGTCACCTTCGGGTGCCTTTTTTATTTCCGAAACGTACCTCAGCAGGTGAATAAATTTTATTCATATTGTTATCAACAAGTTATCAAGTATTTATAATTAAAATGGAAATTGTTTTTGATTTTGCATTTTAAAt                                                                                                                                                                                                           <  1:424914/251‑1 (MQ=255)                                    cAACTGGCTCAACTATTACG‑TCTACCAGATCACGCCATACGATCTACGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCGGGTGCCTTTTTTATTTCCGAAACGTACCTCAGCAGGTGAATAAATTTTATTCATATTGTTATCAACAAGTTATCAAGTATTTATAATTAAAATGGAAATTGTTTTTGATTTTGCATTTTAAAt                                                                                                                                                                                                           >  1:370682/1‑251 (MQ=255)                                         ggCTCAACTATTACG‑TCTACCAGATCACGCCATACGATCTACGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCGGGTGCCTTTTTTATTTCCGCAACGTACCTCAGCAGGTGAATAAATTTTATTCATATTGTTATCAACAAGTTATCAAGTATTTATAATTAAAATGGAAATTGTTTTTGATTTTGCATTTTAAATGagt                                                                                                                                                                                                       >  2:941905/1‑250 (MQ=255)                                                  aTTACG‑TCTACCAGATCACGTCATACGATCTACGGCACATGAATCCAACGCGGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTTCCTCAGGCACCTTCGGGTGCCTTTTTTATTTCCGAAACGTACCTCAGCAGGTGAATAAATTTTATTCATATTGTTATCAACAAGTTATCAAGTATTTATAATTAAAATGGAAATTGTTTTTGATTTTGCATTTTAAATGAGTAGTCTTAGt                                                                                                                                                                                              <  2:945744/250‑1 (MQ=255)                                                    tACG‑TCTAACAGATCACGCCATCCGATCTACGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCGGGTGCCTTTTTTATTTCCGAAACGTACCTCAGCAGGTGAATAAATTTTATTCCTATTGTTATCAACCAGTTATCAAGTATTTATAATTAAAATGGAAATTTTTTTTGATTTTGCATTTTAAATGAGTAGTCTTAGTtgt                                                                                                                                                                                           >  2:90486/1‑251 (MQ=255)                                                                 agcaagCCATACGATCTACGGCACATGAATCCAACGCTGGAGTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCATGCACCTTCGGGTGCCTTTTTTATTTCCGAAACGTACCTCAGCAGGTGAATAAATTTTATTCATATTGTTATCAACAAGTTATGAAGTAGTTATAATTAAAATGGAAATTGTTTTTGATTTTGCATTTTAAATGAGTAGTCTTAGTTGGGCTGAACGaaaa                                                                                                                                                                               <  2:825839/246‑1 (MQ=255)                                                                        cATACGATCTACGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCGGGTGCCTTTTTTATTTCCGAAACGTACCTCAGCAGGTGAATAAATTTTATTCATATTGTTATCAACAAGTTATCAAGTATTTATAATTAAAATGGAAATTGTTTTTGATTTTGCATTTTAAATGAGTAGTCTTAGTTGTGCTGAACGAAAAGAGcaca                                                                                                                                                                        <  1:924684/251‑1 (MQ=255)                                                                         aTACGATCTACGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCGGGTGCCTTTTTTATTTCCGAAACGTACCTCAGCAGGTGAATAAATTTTATTCATATTGTTATCAACAAGTTATCAAGTATTTATAATTAAAATGGAAATTGTTTTTGATTTTGCATTTTAAATGAGTAGTCTTAGTTGTGCTGAACGAAAAGAGCACaa                                                                                                                                                                       <  1:462143/251‑1 (MQ=255)                                                                          tACGATCTACGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCGGGTGCCTTTTTTATTTCCGAAACGTACCTCAGCAGGTGAATAAATTTTATTCATATTGTTATCAACAAGTTATCAAGTATTTATAATTAAAATGGAAATTGTTTTTGATTTTGCATTTTAAATGAGTAGTCTTAGTTGTGCTGAACGAAAAGAGCACaa                                                                                                                                                                       <  2:343490/250‑1 (MQ=255)                                                                                  aCGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCGGGTGCCTTTTTTATTTCCGAAACGTACCTCAGCAGGTGAATAAATTTTATTCATATTGTTATCAACAAGTTATCAAGTATTTATAATTAAAATGGAAATTGTTTTTGATTTTGCATTTTAAATGAGTAGTCTTAGTTGTGCTGAACGAAAAGAGCACAACGATCCtt                                                                                                                                                               <  2:702715/250‑1 (MQ=255)                                                                                          tGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCGGGTGCCTTTTTTATTTCCGAAACGTACCTCAGCAGGTGAATAAATTTTATTCATATTGTTATCAACAAGTTATCAAGTATTTATAATTAAAATGGAAATTGTTTTTGATTTTGCATTTTAAATGAGTAGTCTTAGTTGTGCTGAACGAAAAGAGCACAACGATCCTTCGTTcac                                                                                                                                                        >  1:555985/1‑249 (MQ=255)                                                                                           gaaaccAACGCTGGATTTATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCTCCTTTCCTCAGGCACCTTCGGGTGCCTTTTTTATTTCCGAAACGTACCTCAGCAGGTGAATAAAATTTATTCATATTGTTATCAACAAGTTATCAAGTATTTATAATTAAAATGGAAATTTTTTGTGATTTTGCATTTTAAATGAGTAGTCTTAGTTGTGCTGCACGAAAAGAGCACAACGCTCCTTCGTTCACAGt                                                                                                                                                     <  2:791955/247‑1 (MQ=255)                                                                                                    gCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCGGGTGCCTTTTTTATTTCCGAAACGTACCTCAGCAGGTGAATAAATTTTATTCATATTGTTATCACCAAGTTATCAAGTATTTATAATTAAAATGGAAATTGTTTTTGATTTTGCATTTTAAATGAGTAGTCTTAGTTGTGCTTAACGAAAAGAGCACAACGATCCTTCGTTCACAGTGGGGAAGtt                                                                                                                                            >  1:236766/1‑251 (MQ=255)                                                                                                         attaatcgtcggttagattCGATCGTTATGTGATTCAGCACCTTATCTCAGTCACCTTCGGGGTCCTTTTTTATTGCCGCAACGTACCTCATCAGGTGAAGAAATTTTATTTATATTGTTATCAACAAGTTTTCAAGTATTTACTATTAAATTGGAAATTGTTTTTGATTTTGCATTTTAAATGAGTAGTCTGAGTTTTGCTGAACGAAAAGAGCACGACTATCCTACGTTCACAGTGGGAAAGTTTTCgg                                                                                                                                       <  2:764991/233‑1 (MQ=255)                                                                                                                    gtgATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCGGGTGCCTTTTTTATTTCCGAAACGTACCTCAGCAGGTGAATAAATTTTATTCATATTGTTATCAACAAGTTATCAAGTATTTATAATTAAAATGGAAATTGTTTTTGATTTTGCATTTTAAATGAGTAGTCTTAGTTGTGCTGAACGAAAAGAGCACAACGATCCTTCGTTCACAGTGGGGAAGTTTTCGGATCCATGAc                                                                                                                              <  1:386605/249‑1 (MQ=255)                                                                                                                       aTAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCGGGTGCCTTTTTTATTTCCGAAACGTACCTCAGCAGGTGAATAAATTTTATTCATATTGTTATCAACAAGTTATCAAGTATTTATAATTAAAATGGAAATTGTTTTTGATTTTGCATTTTAAATGAGTAGTCTTAGTTGTGATGAACGAAAAGAGCACAACGATCCTTCGTTCACAGTGGGGAAGTTTTCGGATCCATGACgagga                                                                                                                         <  1:466283/251‑1 (MQ=255)                                                                                                                                cGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCGGGTGCCTTTTTTATTTCCGAAACGTACCTCAGCAGGTGAATAAATTTTATTCATATTGTTATCAACAAGTTATCAAGTATTTATAATTAAAATGGAAATTGTTTTTGATTTTGCATTTTAAATGAGTAGTCTTAGTTGTGCTGAACGAAAAGAGCACAACGATCCTTCGTTCACAGTGGGGAAGTTTTCGGATCCATGACGAGGAGCTGCACg                                                                                                                 >  2:261419/1‑250 (MQ=255)                                                                                                                                cGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCGGGGGCCTTTTTTATTTCCGAAACGTACCTCAGCAGGTGAATAAATTTTATTCATATTGTTATCAACAAGTTATCAAGTATTTATAATTAAAATGGAAATTGTTTTTGATTTTGCATTTTAAATGCGTAGTCTTAGTTGTGCTGAACGAAAAAAGCACAACAATCCTTCGTTCACAGTGGGGAAGGTTTCGGATCCATGCCGAGGAGCTGCACg                                                                                                                 >  1:759881/1‑250 (MQ=255)                                                                                                                                 gTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCGGGTGCCTTTTTTATTTCCGAAACGTACCTCAGCAGGTGAATAAATTTTCTTCATATTGTTATCAACAAGTTATCAAGTATTTATAATTAAAATGGAAATTGTTTTTGATTTTGCATTTTAAATGATTAGTCTTAGTTGTTCTGAACGAAAAGAGCACACCGATCCTTCGTTCACAGTGGGGACGTTTCCGGATCCATGACGTGGAGCTGCACGAt                                                                                                               >  2:655601/1‑251 (MQ=255)                                                                                                                                             aGCACCTTACCTCAGGCACCTTCGGGTGCCTTTTTTATTTCCGAAACGTACCTCAGCAGGTGAATAAATTTTATTCATATTGTTCTCAACAAGTTATCAAGTATTTATAATTAAAATGGATATTGTTTTTGATTTTGCATTTTAAATGAGTAGTCTTAGTTGTGCTGAACGAAAAGCGCACAACGATCCTTCGTTCACAGTGGGGAAGTTTTCGGATCCATGACGAGGAGCTGCACGCTGACTGAACAGGc                                                                                                   >  2:102495/1‑251 (MQ=255)                                                                                                                                                 ccTTACCGCAGGCACCTTCGGGTGCCTTTTTTATTTCCGAAACGTACCTCAGCAGGTGAATAAATTTTATTCATATTGTTATCAACAAGTTATCAAGTATTTATAATTAAAATGGAAATTGTTTTTGATTTTGCATTTTAAATGAGTAGTCTTAGTTGTGCTGAACGAAAAGAGCACAACGATCCTTCGTTCACAGTGGGGAAGTTTTCGGATCCATGACGAGGAGCTGCACGATGACTGAACAGGcaaca                                                                                               <  1:90486/251‑1 (MQ=255)                                                                                                                                                         cAGGCACCTTCGGGTGCCTTTTTTATTTCCGAAACGTACCTCAGCAGGTGAATAAATTTTATTCATATTGTTATCAACAAGTTATCAAGTATTTATAATTAAAATGGAAATTGTTTTTGATTTTGCATTTTAAATGAGTAGTCTTAGTTGTGCTGAACGAAAAGAGCACAACGATCCTTCGTTCACAGTGGGGAAGTTTTCGGATCCATGACGAGGAGCTGCACGATGACTGAACAGGCAACAACAACCg                                                                                        >  2:672857/1‑250 (MQ=255)                                                                                                                                                               ccTTCGGGTGCCTTTTTTATTTCCGAAACGTACCTCAGCAGGTGAATAAATTTTATTCATATTGTTATCAACAAGTTATCAAGTATTTATAATGAAAATGGAAATTGTTTTTGATTTTGCATTTTAAATGAGTAGTCTTAGTTGTGCTGAACGAAAAGAGCACAACGATCCTTCGTTCACAGTGGGGAAGTTTTCGGATCCATGACGAGGAGCTGCACGATGACTGAACAGGCAACAACAACCGATGAACt                                                                                 <  2:818046/251‑1 (MQ=255)                                                                                                                                                                  tCGGGTGCCTTTTTTATTTCCGAAACGTACCTCAGCAGGTGAATAAATTGTATTCATATTGTTATCAACAAGTTATCAAGTATTTATAATTAAAATGGAAATTGTTTTTGATTTTGCATTTTAAATGAGTAGTCTTAGTTGTGCTGAACGAAAAGAGCACAACGATCCTTCTGTCACAGTGGGGAAGTTTTCGGATCCATGACGAGGAGCTGCACGATGACTGAACAGGCAACAACAACCGATGAACTgg                                                                               <  1:926019/250‑1 (MQ=255)                                                                                                                                                                        gCCTTTTTTATTTCCGAAACGTACCTCAGCAGGTGAATAAATTTTATTCATATTGTTATCAACAAGTTATCAAGTATTTATAATTAAAATGGAAATTGTTTTTGATTTTGCATTTTAAATGAGTAGTCTTAGTTGTGCTGAACGAAAAGAGCACAACGATCCTTCGTTCACAGTGGGGAAGTTTTCGGATCCATGACGAGGAGCTGCACGATGACTGAACAGGCAACAACAACCGATGAACTGGCTTTcac                                                                        <  2:468787/251‑1 (MQ=255)                                                                                                                                                                          cTTTTTTATTTCCGAAACGTACCTCAGCAGGTGAATAAATTTTATTCATATTGTTATCAACAAGTTATCAAGTATTTATAATTAAAATGGAAATTGTTTTTGATTTTGCATTTTAAATGAGTAGTCTTAGTTGTGCTGAACGAAAAGAGCACAACGATCCTTCGTTCACAGTGGGGAAGTTTTCGGATCCATGACGAGGAGCTGCACGTTGCCTGAACAGGCAACAACAACCGATGAACTGGCTTTcaca                                                                       >  2:364478/1‑250 (MQ=255)                                                                                                                                                                            tttttATTTCCGAAACGTACCTCAGCAGGTGAATAAATTTTATTCATATTGTTATCAACAAGTTATCAAGTATTTATAATTAAAATGGAAATTGTTTTTGATTTTGCATTTTAAATGAGTAGTCTTAGTTGTGCTGAACGAAAAGAGCACAACGATCCTTCGTTCACAGTGGGGAAGTTTTCGGATCCATGACGAGGAGCTGCACGATGACTGAACAGGCAACAACAACCGATGAACTGGCTTTCACAAg                                                                     <  2:126913/250‑1 (MQ=255)                                                                                                                                                                             ttttATTTCCGAAACGTACCGCAGCAGGTGAATAAATTTTATTCATATTGTTATCAACAAGTTATCAAGTATTTATAATTAAAATGGAAATTGTTTTTGATTTTGCATTTTAAATGAGTAGTCTTAGTTGTGCTGAACGAAAAGAGCACAACGATCCTTCGTTCACAGTGGAGAAGTTTTCGGATCAATGACGAGGAGCTGCACGATGACTGAACAGGCAACAACAAACGATGAACTGGCTTACGCAAGGc                                                                   >  2:804054/1‑251 (MQ=255)                                                                                                                                                                                tATTTCCGAAACGTACCTCAGCAGGTGAATAAATTTTATTCATATTGTTATCAACAAGTTATCAAGTATTTATAATTAAAATGGAAATTGTTTTTGATTTTGCATTTTAAATGAGTAGTCTTAGTTGTGCTGAACGAAAAGAGCACAACGATCCTTCGTTCACAGTGGGGAAGTTTTCGGATCCATGACGAGGAGCTGCACGATGACTGAACAGGCAACAACAACCGATGAACTGGCTTTCACAAGGCCGt                                                                >  1:383328/1‑251 (MQ=255)                                                                                                                                                                                     ccGAAACGTACCTCAGCAGGTGAATAAATTTTATTCATATTGTTATCAACAAGTTATCAAGTATTTATAATTAAAATGGAAATTGTTTTTGATTTTGCATTTTAAATGAGTAGTCTTAGTTGTGCTGAACGAAAAGAGCACAACGATCCTTCGTTCACAGTGGGGAAGTTTTCGGATCCATGACGAGGAGCTGCACGATGACTGAACAGGCAACAACAACCGATGAACTGGCTTTCACAAGGCCGTATGGc                                                           <  1:30571/251‑1 (MQ=255)                                                                                                                                                                                          aCGTACCTCAGCAGGTGAATAAATTTTATTCATATTGTTATCAACAAGTTATCAAGTATTTATAATTAAAATGGAAATTGTTTTTGATTTTGCATTTTAAATGAGTAGTCTTAGTTGTGCTGAACGAAAAGAGCACAACGATCCTTCGTTCACAGTGGGGAAGTTTTCGGATCCATGACGAGGAGCTGCACGATGACTGAACAGGCAACAACAACCGATGAACTGGCTTTCACAAGGCCGTATGGCGAGc                                                       >  1:178167/1‑250 (MQ=255)                                                                                                                                                                                           ccttcctcagcaaggaaaTAAATTTTATTCATATTGTTATCAAAAAGTTATCAAGTATTTATAATTAAAATGGAAATTGTTTTTTATTTTTCATTGTAAATGAGTAGTCTTAGTTTTGCTGAACGAAAAGAGCACAACGATCCTTCGTTCACAGTGGGGAAGTTTGCGGATCCATGACGAGGAGCTGCACGATGACTGAACAGGCAACAACAACCGATGAACTGGCTTTCACAAGGCCGTATGGCGAGCAg                                                     <  2:914490/235‑1 (MQ=255)                                                                                                                                                                                                  cagcagGTGAATAAATTTTAGTCATATTGTTATCAACAAGTTATCAAGTATTTATAATTAAAATGGAAATTGTTTTTGATTTTGCATTTTAAATGAGTAGTCTTAGTTGTGCTGAACGAAAAGAGCACAACGATCCTTCGTTCACAGTGGGGAAGTTTTCGGATCCATGACGAGGAGCTGCACGATGACTGAACAGGCAACAACAACCGATGAACTGGCTTTCACAAGGCCGTATGGCGAGCAGGAGaa                                                <  2:748730/249‑1 (MQ=255)                                                                                                                                                                                                     cagGTGAATAAATTTTATTCATATTGTTATCAACAAGTTATCAAGTATTTATAATTAAAATGGAAATTGTTTTTGATTTTGCATTTTAAATGAGTAGTCTTAGTTGTGCTGAACGAAAAGAGCACAACGATCCTTCGTTCACAGTGGGGAAGTTTTCGGATCCATGACGAGGAGCTGCACGATGACTGAACAGGCAACAACAACCGATGAACTGGCTTTCACAAGGCCGTATGGCGAGCAGGAGAAGCaa                                            >  1:306568/1‑250 (MQ=255)                                                                                                                                                                                                                  tttATTCATATTGTTATCAACAAGTTATCAAGTATTTATAATTAAAATGGAAATTGTTTTTGATTTTGCATTTTAAATGAGTAGTCTTAGTTGTGCTGAACGAAAAGAGCACAACGATCCTTCGTTCACAGTGGGGAAGTTTTCGGATCCATGACGAGGAGCTGCACGATGACTGAACAGGCAACAACAACCGATGAACTGGCTTTCACAAGGCCGTATGGCGAGCAGGAGAAGCAAATTCTTACTGCCg                               <  1:164801/250‑1 (MQ=255)                                                                                                                                                                                                                     aTTCATATTGTTATCAACAAGTTATCAAGTATTTATAATTAAAATGGAAATTGTTTTTGATTTTGCATTTTAAATGAGTAGTCTTAGTTGTGCTGAACGAAAAGAGCACAACGATCCTTCGTTCACAGTGGGGAAGTTTTCGGATCCATGACGAGGAGCTGCACGATGACTGAACAGGCAACAACAACCGATGAACTGGCTTTCACAAGGCCGTATGGCGAGCAGGAGAAGCAAATTCTTACTGCCGAAGc                           <  1:655601/251‑1 (MQ=255)                                                                                                                                                                                                                            ttGTTATCAACAAGTTATCAAGTATTTATAATTAAAATGGAAATTGTTTTTGATTTTGCATTTTAAATGAGTAGTCTTAGTTGTGCTGAACGAAAAGAGCACAACGATCCTTCGTTCACAGTGGGGAAGTTTTCGGATCCATGACGAGGAGCTGCACGATGACTGAACAGGCAACAACAACCGATGAACTGGCTTTCACAAGGCCGTATGGCGATCAGGAGCAGCAAATTCTTACTGCAGAAGCGGTAGa                     >  1:921504/1‑250 (MQ=255)                                                                                                                                                                                                                              gttagcaactagtaaacAAGTATTTATAATTAAAATGGAAATTGTTTTTGATTGTGCAGTTTAAATGAGTAGTCTTAGTTGTGATTAACGAAAAGAGCACCACGATCCTTCGTTGACAGTGGGGAAGTTTTCGTATCCATGACGAGGAGCTGCACGATGACTGAACAGGCAACAACAACCGATGAACTTGCTTTCACAAGTCCGTATGGCGAGCAGGAGAAGCAAATTCTTACTGCCGAAGTGGTAGAAt                   <  2:36592/234‑1 (MQ=255)                                                                                                                                                                                                                                tATCAACAAGTTATCAAGTATTTATAATTAAAATGGAAATTGTTTTTGATTTTGCATTTTAAATGAGTAGTCTTAGTTGTGCTGAACGAAAAGAGCACAACGATCCTTCGTTCACAGTGGGGAAGTTTTCGGATCCATGACGAGGAGCTGCACGATGACTGAACAGGCAACAACAACCGATGAACTGGCTTTCACAAGGCCGTATGGCGAGCAGGAGAAGCAAATTCTTACTGCCGAAGCGGTAGAAttt                 <  1:756182/250‑1 (MQ=255)                                                                                                                                                                                                                                 aTCAACAATTTATCAAGTATTTATAATTAAAATGGAAATTTGTTTTGATGTTTCATTGTAAATGAGTAGTCTTAGTTTTGCTGAACGAAAAGAGCACATCGATCCTTCGTTCACAGTGGGGAAGTTTTCGGATCCATGACGAGGAGCTTCACGATGACTGAACAGGCAACAACAACCGATGAACTGGCTTTCACAAGGCCGTATGGCGAGCAGGAGAATCAAATTCTTACTTCCGAAGCGGTATAATTTc                <  2:1648/250‑1 (MQ=255)                                                                                                                                                                                                                                   aaacaaGTTAACAAGTATTTATAATTACAATGGAAATTGTTTTTGATTTTGCATTTTAAATGAGTAGTCTTAGTTGTGCTGAACGAAAAGAGCACAACGATCCTTCGTTCACAGTGGGGAAGTTTTCGGATCCATGACGAGGAGCTGCACGATGACTGAACAGGCAACAACAACCGATGAACTGGCTTTCACAAGGCCGTATGGCGAGCAGGAGAAGCAAATTCTTACTGCCGAAGCGGTAGAATTTctga             <  2:677906/250‑1 (MQ=255)                                                                                                                                                                                                                                           tATCAAGCATTTATAATTAAAATGGAAATTGTTTTTGATTTTGCATTTTAAATGAGTAGTCTTAGTTGTGCTGAACGAAAAGAGCACAACGATCCTTCGTTCACAGTGGGGAAGTTTTCGGATCCATGACGAGGAGCTGCACGATGACTGAACAGGCAACAACAACCGATGAACTGGCTTTCACAAGGCCGTATGGCGAGCAGGAGAAGCAAATTCTTACTGCCGAAGCGGTAGAATTTCTGACTGAGCTg     >  1:702111/1‑251 (MQ=255)                                                                                                                                                                                                                                               aaGTATTTATAATTAAAAGGGAAATTGTTTGTGAGTTTTCAGGTTAAATTAGGATCCGTAGTTGGGCTGAACGAAAAGAGCACAATGATCCTTCGTTCACAGTGGGGAAGTTTTCGGCTCCATGACGAGGAGCTGCCCGATGCCTGAACATGCAACAACAACCGATGAACTGGCTTTCACAAGGCCGTATGGCGAGCAGGAGAAGCAAATTCTTACTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTg  <  2:545945/250‑1 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                  GCGAGCTGGCGTAGTCACGGTAATTTACTGTTTACCAACTGGCTCAACTATTACG‑TCTACCAGATCACGCCATACGATCTACGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCGGGTGCCTTTTTTATTTCCGAAACGTACCTCAGCAGGTGAATAAATTTTATTCATATTGTTATCAACAAGTTATCAAGTATTTTTAATTAAAATGGAAATTGTTTTTGATTTTGCATTTTAAATGAGTAGTCTTAGTTGTGCTGAACGAAAAGAGCACAACGATCCTTCGTTCACAGTGGGGAAGTTTTCGGATCCATGACGAGGAGCTGCACGATGACTGAACAGGCAACAACAACCGATGAACTGGCTTTCACAAGGCCGTATGGCGAGCAGGAGAAGCAAATTCTTACTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTG  >  NC_000913/4215102‑4215588

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑