Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,084,903 T→G N307T (AAC→ACC)  fdoG ← formate dehydrogenase O subunit alpha

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,084,9030TG96.2% 434.2 / ‑2.0 133N307T (AAC→ACC) fdoGformate dehydrogenase O subunit alpha
Reads supporting (aligned to +/- strand):  ref base T (2/3);  new base G (61/67);  total (63/70)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01

Only 100 of 133 total aligned reads displayed.

TTTCAGGAACGCGTCTTTTGGCGTACCACAGATGTTTTCAACCACATCTGGCGTGTAGCGGGAAACGTGCTGTTTCAGCAAGTTCCACACGCAGCGCGGATGTTGCAGCGTGGTATCGCGTTTGGCGAAGCCGTTTTCGTCCAGT‑TCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGTTGGTATAGGCT‑TCGGTG‑TATTCGCGGTTGAATTTTTCATTGTTCAGCAG‑GTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCATAGTAGTCAGCTACCGCCGCCGT‑ACGCGTAAAGCGAGGATCGATCACAATCAGCTTCGCGCCGTTGTGAATTTTGGCTTCCATCGCCCAGCGGAACCCGACCGGGTGAGCTTCAGCGGCGTTACCGCCCATCACCACGACGAGGT  >  NC_000913/4084656‑4085152
                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                             
tttCAGGAACGCGTCTTTTGGCGTACCACAGATGTTTTCAACCACATCTGGCGTGTAGCGGGAAACGTGCTGTTTCAGCAAGTTCCACACGCAGCGCGGATGTTGCAGCGTGGTATCGCGTTTGGCGAAGCCGTTTTCGTCCAGT‑TCATACGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCCCGATCAGGCTGGCGGTgg                                                                                                                                                                                                                                                            >  2:305602/1‑251 (MQ=255)
   cAGGAACGCGTCTTTTGGCGTACCACAGATGTTGTCAACCACATCTGGCGTGTAGCGGGAATCGTGCTGTTTCAGCAAGTTCCACACGCAGCGCGGATGTTGCAGCGTGGTATCGCGTTTGGCGAAGCCGTTTTCGTCCAGT‑TCATAAGCCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCAGCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGGTGGta                                                                                                                                                                                                                                                          <  1:327759/250‑1 (MQ=255)
    aGGAACGCGTCTTTTGGCGTACCACAGATGTTTTCAACCACATCTGGCGTGTAGCGGGAAACGTGCTGTTTCAGCAAGTTCCACACGCAGCGCGGATGTTGCAGCGTGGTATCGCGTTTGGCGAAGCCGTTTTCGTCCAGT‑TCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGGTGGtata                                                                                                                                                                                                                                                        <  1:159996/251‑1 (MQ=255)
    aGGAACGCGTCTTGTTGCTTACCACAGATGTTTTCAACCCCACCTGGCGTGTAGCGGGAAACGGGCGGTTTCAGCAAGTTCCACCCGCAGCGCGGATGTTGCTGCGTGGTATCGCGTTTGGCGAAGCCGTTTTCGTCCAGT‑TCATAAGTCCAGGAGGATTTAGCGGACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGGTGGtata                                                                                                                                                                                                                                                        <  2:389152/251‑1 (MQ=255)
        aCGCGTCTTTTGGCGTACCACAGATGTTTTCAACCACATCTGGCGTGTAGCGGGAAACGTGCTGTTTCAGCAAGTTCCACACGCAGCGCGGATGTTGCAGCGTGGTATCGCGTTTGGCGAAGCCGTTTTCGTCCAGT‑TCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCCCGATCAGGCTGGAGGTGGTAAAGGCt                                                                                                                                                                                                                                                    >  2:681724/1‑251 (MQ=255)
         cgcgTCTTTTGGCGTACCACAGATGTTTTCAACCACATCTGGCGTGTAGCGGGAAACGTGCTGTTTCAGCAAGTTCCACACGCAGCGCGGATGTTGCAGCGTGGTATCGCGTTTGGCGAAGCCGTTTTCGTCCAGT‑TCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGGTGGTATAgggtt                                                                                                                                                                                                                                                   >  2:476867/1‑248 (MQ=255)
          gcgTCTTTTGGCGTACCCCAGATGTTTTCAACCACATCTGGCGTGTAGCGGGAAACGTGCTGTTTCAGCAAGTTCCACACGCAGCGCGGATGTTGCAGCGTTGTATCGCGTTTGGCGAAGCCGTTTTCGTCCAGT‑TCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGGTGGTATAGGCT‑Tc                                                                                                                                                                                                                                                 <  2:979263/251‑1 (MQ=255)
            gTCTTTTGGCGTACCACAGATGTTTTCAACCACATCTGGCGTGTAGCGGGAAACGTGCTGTTTCAGCAAGTTCCACACGCAGCGCGGATGTTGCAGCGTGGTATCGCGTTTGGCGAAGCCGTTTTCGTCCAGT‑TCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGGGGGTATAGGCT‑TCgg                                                                                                                                                                                                                                               >  1:394082/1‑251 (MQ=255)
             tCTTTTGGCGTACCACAGATGTTTTCAACCACATCTGGCGTGTAGCGGGAAACGTGCTGTTTCAGCAAGTTCCACACGCAGCGCGGATGTTGCAGCGTGGTATCGCGTTTGGCGAAGCCGTTTTCGTCCAGT‑TCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGGTGGTATAGGCT‑TCggg                                                                                                                                                                                                                                              >  1:136907/1‑250 (MQ=255)
              cTTTTGGCGTACCACAGATGTTTTCAACCACATCTGGCGTGTAGCGGGAAACGTGCTGTTTCAGCAAGTTCCACACGCAGCGCGGATGTTGCAGCGTGGTATCGCGTTTGGCGAAGCCGTTTTCGTCCAGT‑TCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGGTGGTATAGGCT‑TCGgtg                                                                                                                                                                                                                                             >  1:43279/1‑251 (MQ=255)
                tttGGCGTACCACAGATGTTTTCAACCACATCTGGCGTGTAGCGGGAAACGTGCTGTTTCAGCAAGTTCCACACGCAGCGCGGATGTTGCAGCGTGGTATCGCGTTTGGCGAAGCCGTTTTCGTCCAGT‑TCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGGTGGTATAGGCT‑TCGgtg‑t                                                                                                                                                                                                                                           <  1:186970/250‑1 (MQ=255)
                   ggCGTACCACAGATGTTTTCAACCACATCTGGCGTGTAGCGGGAAACGTGCTGTTTCAGCAAGTTCCACACGCAGCGCGGATGTTGCAGCGTGGTATCGCGTTTGGCGAAGCCGTTTTCGTCCAGT‑TCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGGTGGTATAGGCT‑TCGGTG‑TATTc                                                                                                                                                                                                                                       <  1:935736/251‑1 (MQ=255)
                   ggCGTACCACAGATGTTTTCAACCACATCTGGCGTGGAGCGGGAAACGTGCTGTTTCAGCAAGTTCCACACGCAGCGCGGATGTTGCAGCGTGGTATCGCGTTTGGCGAAGCCTTTTTCGTCCAGT‑TCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGGTGGTATAGGCT‑TCGGTG‑TATTc                                                                                                                                                                                                                                       <  2:342293/251‑1 (MQ=255)
                     cGTACCACAGATGTTTTCAACCACATCTGGCGTGTAGCGGGAAACGTGCTGTTTCAGCAAGTTCCACACGCAGCGCGGATGTTGCAGCGTGGTATCGCGTTTGGCGAAGCCGTTTTCGTCCAGT‑TCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGGTGGTATAGGCT‑TCGGTG‑TATTcgc                                                                                                                                                                                                                                     >  1:563620/1‑251 (MQ=255)
                         ccACAGATGTTTTCAACCACATCTGGCGTGTAGCGGGAAACGTGCTGTTTCAGCAAGTTCCACACGCAGCGCGGATGTTGCAGCGTGGTATCGCGTTTGGCGAAGCCGTTTTCGTCCAGT‑TCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGGTGGTATAGGCT‑TCGGTG‑TATTCGCGGtt                                                                                                                                                                                                                                 <  1:476867/251‑1 (MQ=255)
                          cacaGATGTTTTCAACCACATCTGGCGTGTAGCGGGAAACGTGCTGTTTCAGCAAGTTCCACACGCAGCGCGGATGTTGCAGCGTGGTATCGCGTTTGGCGAAGCCGTTTTCGTCCAGT‑TCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGGTGGTATAGGCG‑TCGGTG‑TATTCGCGGTTg                                                                                                                                                                                                                                <  1:251926/251‑1 (MQ=255)
                          cacaGATGTTGTCAACCACATCTGGCGTGTAGCGGGAAACGTGCTGTTTCAGCAAGTTCCACACGCAGCGCGGATGTTGCAGCGTGGTATCGCGTTTGGCGAAGCCGATTTCGTCCAGT‑TCATAAGTCCAGGAGGATTTATCGTACTTGCGGTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGGTGGGATAGGCT‑TCggggtcttctcgggtg                                                                                                                                                                                                                                 >  2:155575/1‑237 (MQ=255)
                              gATGTTTTCAACCACATCTGGCGTGTAGCGGGAAACGTGCTGTTTCAGCAAGTTCCACACGCAGCGCGGATGTTGCAGCGTGGTATCGCGTTTGGCGAAGCCGTTTTCGTCCAGT‑TCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGGTGGTATAGGCT‑TCGGTG‑TATTCGCGGTTGAAtt                                                                                                                                                                                                                            >  1:699853/1‑251 (MQ=255)
                                        aCCACATCTGGCGTGTAGCGGGAAACGTGCTGTTTCAGCAAGTTCCACACGCAGCGCGGATGTTGCAGCGTGGTATCGCGTTTGGCGAAGCCGTTTTCGTCCAGT‑TCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGGTGGTATAGGCT‑TCGGTG‑TATTCGCGGTTGAATTTTTCATTGtt                                                                                                                                                                                                                  <  1:92429/251‑1 (MQ=255)
                                         ccACATCTGGCGTGTAGCGGGAAACGTGCTGTTTCAGCAAGTTCCACACGCAGCGCGGATGTTGCAGCGTGGTATCGCGTTTGGCGAAGCCGTTTTCGGCCAGT‑TCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGGTGGTATAGGCT‑TCGGTG‑TATTCGCGGTTGAATTTTTCATTGTTc                                                                                                                                                                                                                 <  2:699853/251‑1 (MQ=255)
                                                gggCGTGGAGCGGGAAACGTGCTGTTTCAGCAAGTTCCACACGCAGCGTGGATGTTGCAGCGTGGTATCGCGTTTGGCGAAGCCGTTTTCGTCCAGG‑TCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGGTGGTATAGGCT‑TCGGTG‑TATTCGCGGTTGAATTTTTCATTGTTCAGCAG‑Gt                                                                                                                                                                                                         <  2:382784/250‑1 (MQ=255)
                                                    gtgtAGCGGGAAACGTGCTGTTTCAGCAAGTTCCACACGCAGCGCGGATGTTGCAGCGTGGTATCGCGTTTGGCGAAGCCGTTTTCGTCCAGT‑TCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTAACGCACGATCAGGCTGGCGGTGGTATAGGCT‑TCGGTG‑TATTCGCGGTTGAATTTTTCATTGTTCAGCAG‑GTACAg                                                                                                                                                                                                     <  1:425674/251‑1 (MQ=255)
                                                    gtgacgCGGGAAACGTGATGTTTCAGCCAGTTCCACACGCAGCGCGGATGTTGAAGCGTGGTATCGCGTATGGCGAAGCCGTTTTCGGCCAGT‑TCATCAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGAGTCGTAGCCGGTGAAAAGGCCATCTTCAAAGCCGTCATCCTCACGCACGATCAGGCTGGCGGGGGTAGAAGCT‑TCGGTG‑GATTCGCGGTTGAATTTTTCATTGTTcagacggtac                                                                                                                                                                                                        >  2:571161/6‑242 (MQ=255)
                                                            ggacaCGTGCTGTTTCAGCAAGTTCCACACGCAGCGCGGATGTTGCAGCGTGGTATCGCGTTTGGCGAAGACGTTTTCGTCCAGT‑TCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGGGGGTATAGGGT‑TCGGGG‑TATTCGCGGTTGGATTTTTCATTGGTCAGCAG‑Gtcaagcaaaacac                                                                                                                                                                                             >  2:184170/5‑239 (MQ=255)
                                                              aaaCGGGCTGTTTCAGCACGTTCCACACGCATCGCGGATGTTGCAGCGTGGTATCGCGTTTGGCGACCCCGTTTTCGTCCGGT‑TCATAAGACCAGGAGGCTTTATCGTACTTGCGTTTTTCAGCGTAGTAGCCGGTGAACAGGCCATCTTAAAAGCCGTACTCCTCACGCACGATCAGGCTGGCGGGGGGATAGGCT‑TTGGGT‑TTTTCCGGGTTGATTTTTTCATTggtccgcacaaaatgcaatatccct                                                                                                                                                                                            >  2:87680/1‑227 (MQ=255)
                                                                aCGTGCTGTTTCAGCAAGTTCCACACGCAGCGCGGATGTTGCAGCGTGGTATCGCGTTTGGCGAAGCCGTTTTCGTCCAGT‑TCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGGTGGTATAGGCT‑TCGGTG‑TATTCGCGGTTGAATTTTTCATTGTTCAGCAG‑GTACAGCAATACGCCTGa                                                                                                                                                                                         <  1:289946/251‑1 (MQ=255)
                                                                aCGTGCTGTTTCAGCAAGTTCCACACGCAGCGCGGATGTTGCAGCGTGGTATCGCGTTTGGCGAAGCCGTTTTCGTCCAGT‑TCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGGTGGTATAGGCT‑TCGGTG‑TATTCGCGGTTGAATTTTTCATTGTTCAGCAG‑GTACAGCAATACGCCTGa                                                                                                                                                                                         <  1:336749/251‑1 (MQ=255)
                                                                   tGCTGTTTCAGCAAGTTCCACACGCAGCGCGGATGTTGCAGCGTGGTATCGCGTTTGGCGAAGCCGTTTTCGTCCAGT‑TCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGGTGGTATAGGCT‑TCTGTG‑TATTCGAGGTTGAATTTTTCATTGTTCAGCAG‑GTACAGCACTACGCCTGAc                                                                                                                                                                                        >  1:869375/1‑249 (MQ=255)
                                                                     cTGTTTCAGCAAGTTCCACACGCAGCGCGGATGTTGCAGCGTGGTATCGCGTTTGGCGAAGCCGTTTTCGTCCAGT‑TCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGGTGGGATAGGCT‑TCGGTG‑TATTCGCGGTTGAATTTTTCATTGTTAAGCAG‑GTACAGCAATACGCCTGACAgg                                                                                                                                                                                     >  1:603375/1‑250 (MQ=255)
                                                                      tGTTTCAGCAAGTTCCACACGCAGCGCGGATGTTGCAGCGTGGTATCGCGTTTGGCGAAGCCGTTTTCGTCCAGT‑TCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGGTGGTATAGGCT‑TCGGGG‑TATTCGCGGTTGAATTTTTCATTGTTCAGCCG‑GTACAGCAATACGCCTgcaagga                                                                                                                                                                                    >  2:119148/1‑244 (MQ=255)
                                                                          tCAGCAAGTTCCACACGCAGCGCGGATGTTGCAGCGTGGTATCGCGTTTGGCGAAGCCGTTTTCGTCCAGT‑TCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGGTGGTATAGGCT‑TCGGTG‑TATTCGCGGTTGAATTTTTCATTGTTCAGCAG‑GTACAGCAATACGCCTGACAGGAAAGc                                                                                                                                                                                <  2:232372/250‑1 (MQ=255)
                                                                            aGCAAGTTCCACACGCAGCGCGGATGTTGCAGCGTGGTATCGCGTTTGGCGAAGCCGTTTTCGTCCAGT‑TCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTT‑CCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGGTGGTATAGGCT‑TCGGTG‑TATTCGCGGTTGAATTTTTCCTTGTTCAGACG‑GGACAGCAACAACCCTGACCGGAAAGCAATg                                                                                                                                                                            >  2:79573/1‑251 (MQ=255)
                                                                               aaGTTCCACACGCAGCGCGGATGTTGCAGCGTGGTATCGCGTTTGGCGAAGCCGTTTTCGTCCAGT‑TCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGTTGGTATAGGCT‑TCGGTG‑TATTCGCGGTTGAATTTTTCATTGTTCAGCAG‑GTACAGCAATACGCCTGACAGGAAAg                                                                                                                                                                                 >  2:167552/1‑244 (MQ=255)
                                                                                aGTTCCACACGCAGCGCGGATGTTGCAGCGTGGTATCGCGTTTGGCGAAGCCGTTTTCGTCCAGT‑TCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGGTGGTATAGGCT‑TCGGTG‑TATTCGCGGTTGTATTTTTCATTGTTCAGCAG‑GTACAGCAATACGCCTGACAGGAAAGCAATggc                                                                                                                                                                          >  1:213168/1‑248 (MQ=255)
                                                                                   tCCACTCGCAGCGCGGCTGGTGCAGCTTGGTAGCTCGTTTGGCGAAGCCGTTTTCGTCCAGT‑TCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAATGCCGTAATCCTCACGCACGATCAGGCTGGCGGTGGTATAGGCT‑TCGGTG‑TATTCGCGGTTGAATTGTTCATTGTTCAGCAG‑GTACAGCAATACGCCTGACATGAAAGCAATGTCAGt                                                                                                                                                                       <  1:478951/250‑1 (MQ=255)
                                                                                   cccACACGCAGCGCGGATGTTGCTGCGTGGTATCGCGTTTGGCGAAGCCGTTTTCGTCCAGT‑TCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGGACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGGTGGTATAGGCT‑TCGGTG‑TATTCGCGGTTGAATTTTTCATTGTTCAGCAG‑GTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTa                                                                                                                                                                      <  2:188267/250‑1 (MQ=255)
                                                                                           cAGCGCGGATGTTGCAGCGTTGTATCGCGTTTGGCGAAGCCGTTTTCGTCCAGT‑TCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGGTGGTATAGGCT‑TCGGTG‑TATTCGCGGTTGAATTTTTCATTGTTCAGCAG‑GTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACg                                                                                                                                                              <  2:83476/251‑1 (MQ=255)
                                                                                              cgcgTATGTTGCAGCGTGGTATCGCGTTTGGCGAAGCCGTTTTCGTCCAGT‑TCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGGTGGTATAGGCT‑TCGGTG‑TATTCGCGGTTGAATTTTTCATTGTTCAGCAG‑GTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAAt                                                                                                                                                           <  1:876500/251‑1 (MQ=255)
                                                                                              cgcgGATGTTGCAGCGTGGTATCGCGTTTGGCGAAGCCGTTTTCGTCCAGT‑TCATAAGTCCAGGAGGAGTTATCGTACTGTCGTTTTTCCGCGCCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGAGCAGGCTGGCGGTGGTATAGGCT‑TCGGTG‑TATTCGCGGTTGAATTTTTCATTGTGCAGCAG‑GGACAGCAATACGCCTGACAGGAAAGCAATTTCAGTACCGGAACGAAt                                                                                                                                                           <  2:451742/251‑1 (MQ=255)
                                                                                              cgcgGATGTTGCAGCGTGGTATCGCGTTCGGCGAAGCCGTTTTCGTCCAGT‑TCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGGGGGGATAGGCT‑TCGGTG‑AATTCGCGGTTGGATTTTTCATTGTTCAGCAG‑GAACAGCAATACGCCTGACAGGAAAGCCAAGTCAGTACCGGAACGaa                                                                                                                                                            >  2:883039/1‑250 (MQ=255)
                                                                                                 ggATGTTGCAGCGTGGTATCGCGTTTGGCGAAGCCGTTTTCGTCCAGT‑TCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGGTGGTATAGGCT‑TCGGTG‑TATTCGCGGTTGGATTTTTCATTTTTCAGCAG‑GGACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAAAAgg                                                                                                                                                        >  2:55345/1‑251 (MQ=255)
                                                                                                   aTGTTGCAGCGTGGTATCGCGTTTGGCGAAGCCGTTTTCGTCCAGT‑TCATAAGTCCAGGAGGATTTAGCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGGTGGTATAGGCT‑TCGGTG‑TATTCGCGGTTGAATTTTTCATTGTTCAGCAG‑GTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAgggg                                                                                                                                                      <  1:616149/251‑1 (MQ=255)
                                                                                                       tGCAGCGTGGTATCGCGTTTGGCGAAGACGTTTTCGTCCAGT‑TCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGACTGGCGGTGGTATAGGCT‑TAGGTG‑GATTCGCGGTTGAATTTTTCATTGGTCAGCAG‑GTAAACAAATCCACCGTACAGGGAAACAATGTCAGTACAGGAACGGAAAggggtat                                                                                                                                                   >  2:889099/1‑247 (MQ=255)
                                                                                                        gCAGCGTGGTATCGCGTTTGGCGAAGCCGTTTTCGTCCAGT‑TCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGGTGGTATAGGCT‑TCGGTG‑TATTCGCGGTTGAATTTTTCATTGTTCAGCAG‑GTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCAtag                                                                                                                                                 <  2:283375/251‑1 (MQ=255)
                                                                                                        gCAGCGTGGTATCGCGTTTGGCGAAGCCGTTTTCGTCCAGT‑TCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGGTGGTATAGGCT‑TCGGTG‑TATTCGCGGTTGAATTTTTCATTGTTCAGCAG‑GTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCAtag                                                                                                                                                 <  1:163561/251‑1 (MQ=255)
                                                                                                        gCAGCGTGGTATCGCGTTTGGCGAAGCCGTTTTCGTCCAGT‑TCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGGTGGGATAGGCT‑TCGGTG‑TATTCGCGGTTGAATTTTTCATTGTTCAGCAG‑GTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCAtag                                                                                                                                                 <  2:984201/251‑1 (MQ=255)
                                                                                                         cAGCGTGGTATCGCGTTTGGCGAAGCCGTTTTCGTCCAGT‑TCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGGTGGTATAGGCT‑TCGGTG‑TATTCGCGGTTGAATTTTTCATTGTTCAGAAG‑GTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCAtagg                                                                                                                                                >  1:540737/1‑250 (MQ=255)
                                                                                                               ggTATCGCGTGTGGCGAAGCCGTTTTCGTCCAGT‑TCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGGTGGTAAAGGAT‑TCGGTG‑TATTCGCGGTTGAATTTTTCCTTGGTTAGCAG‑GGACAGCAACACGGCTGAAAGGAAAGCAATGTCAGTTCCGGAACGACTAGGGGCAtaagaatc                                                                                                                                            >  2:687580/1‑243 (MQ=255)
                                                                                                                    cgcgTTTTGCGAAGGCGTTTTCGTTCAGT‑TCATAAGTCCAGGAGGATTTATCGTTCTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGGTGGTATAGGCT‑TCGGTG‑TATTCGCGGTTGAATTTTTCATTGTTCAGCAG‑GTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCATAGTAGTCAGCTAc                                                                                                                                      <  2:874803/250‑1 (MQ=255)
                                                                                                                    cgcgTTTGGCGAAGCCGTTTTCGTCCAGT‑TCATAGGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGGTGGTATAGGCT‑TCGGTG‑TATTCGCGGTTGAATTTTTCATTGTTCAGCAG‑GTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCATAGTAGTCAGCTAcc                                                                                                                                     <  2:540946/251‑1 (MQ=255)
                                                                                                                    cgcgTTTGGCGAAGCCGTTTTCGTCCAGT‑TCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGGTGGTATAGGCT‑TCGGGG‑TATTCGCGGTTGAATTTTTCATTGTTCAACAC‑GTACGGCAATACGCCTGACAGGAAAGAAATGTCAGGACCGGAACGAATGGGGGGATAGGAGTCAgataac                                                                                                                                     >  2:888704/1‑246 (MQ=255)
                                                                                                                    cgcgTTTGGCGAAGCCGTTTTCGTCCAGT‑TCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATACTCACGCACGATCAGGCTGGGGGTGGTATAGGCT‑TAGGGG‑TATTAGCGGTGGAAATTTTCATTGGTCAGAAG‑GGACAGCAAAAGGACTGACAGGAAAGAAATGGCAGGAACGGGACGaaaaaggggaaaggagtcaggcaa                                                                                                                                      >  2:836143/1‑228 (MQ=255)
                                                                                                                           ggCGAAGCCGTTTTCGTCCAGT‑TCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGGTGGTATAGGCT‑TCGGTG‑TATTCGCGGTTGAATTTTTCATTGTTCAGCAG‑GTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCATAGTAGTCAGCTAccgccgccg                                                                                                                              >  1:25660/1‑251 (MQ=255)
                                                                                                                              gAAGCCGTTTTCTTCC‑GTGTCTTAAGTCCAGGAGGTTTTATCGTACTTTGGTTTTTCCGCGTCGTAGCCGGTGACCAGGCCATCTTCAAAGCCGTAATCCTCATGCACGTTCAGGCGGGCGGTGGTATAGGCT‑GCGGTG‑TATTCGCGGTTTAATTTTTCATTGTTCAGCAG‑GTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCATAGTAGTCAGCTCCCGCCGCCGT‑Ac                                                                                                                          <  2:329578/251‑1 (MQ=255)
                                                                                                                               aaGCCGTTTTCGTCCAGT‑TCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGGTGGTATAGGCT‑TCGGTG‑TATTCGCGGTTGAATTTTTCATTGTTCAGCAG‑GTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCATAGTAGTCAGCTACCGCCGCCGT‑Acg                                                                                                                         <  1:812490/251‑1 (MQ=255)
                                                                                                                                    gTTTTCGTCCAGT‑TCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGGAGGTATAGGCT‑TCGGTG‑TATTCGCGGTTGAATTTTTCATTGTTCAGCAG‑GTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCATAGTAGTCAGCTACCGCCGCCGT‑ACGCGTa                                                                                                                     >  1:56044/1‑250 (MQ=255)
                                                                                                                                       ttCGTCCAGT‑TCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGGTGGTATAGGCT‑TCGGTG‑TATTCGAGGTTGAATTTTTCATTGTTCAGCAG‑GTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCATAGTAGTCAGCTACCGCCGCCGT‑ACGCGTAAAGc                                                                                                                 >  1:70286/1‑251 (MQ=255)
                                                                                                                                                  tcatatgcccAGGGGGTTTTAGCGTACTTGCGTTTTTCCGCGGCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGGTGGTATAGGCT‑TCGGTG‑TATTCGCGGTTGAATTTTTCATTGTTCAGCAG‑GTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTCCCGGAAGGAAGAGGGGCATAGTAGTCAGCTACCGCCGCCGT‑ACGCGTAAAGCGAGgatcga                                                                                                        <  2:171745/242‑1 (MQ=255)
                                                                                                                                                     aaaGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGGTGGTATAGGCT‑TCGGTG‑TATTCGCGGTTGAATTTTTCATTGTTCAGCAG‑GTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCATAGTAGTCAGCTACCGCCGCCGT‑ACGCGTAAAGCGAGGATCGATcac                                                                                                    <  1:95379/250‑1 (MQ=255)
                                                                                                                                                      aaGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGGTGGTATAGGCT‑TCGGTG‑TATTCGCGGTTGAATTTTTCATTGTTCAGCAG‑GTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCATAGTAGTCAGCTACCGCCGCCGT‑ACGCGTAAAGCGAGGATCGATcac                                                                                                    >  1:446699/1‑250 (MQ=255)
                                                                                                                                                      aaGTCCAGGAGGATTTAGCGTACTGGCGTTTTACCGCGTCGTAGCCGGTGAACAGGCCACCTTCAAAGCCGTAATCCTCACGCACGATTAGGCTGTCGGTGGTATAGTCT‑TCGGTT‑TATTCGCGGTTGAATTTTTCATTGTTCAGCAG‑GTACAGCAATACGCCTGACGGGAAATCAATGTCTGTACCGCAACTAATAGGGGCATAGTAGTCAGCTACCGCCGCCGT‑ACGCGTAAAGCGAGGATCGATcaca                                                                                                   <  1:112528/251‑1 (MQ=255)
                                                                                                                                                        gTCCAGTATGATTTTTCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCTATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGGTGGTATAGGCT‑TCGGTG‑TATTCGCGGTTGAATTTTTCATTGTTCAGCAG‑GTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCATAGTAGTCAGCTACCTCCGCCGT‑ACGCGTAAAGCGAGGATCGATCACAAt                                                                                                 <  1:244267/251‑1 (MQ=255)
                                                                                                                                                         tCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGGTGGTATAGGCT‑TCGGTG‑TATTCGCGGTTGAATTTTTCATTGTTCAGCAG‑GTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCATAGTAGTCAGCTACCGCCGCCGT‑ACGCGTAAAGCGAGga                                                                                                            <  2:382335/239‑1 (MQ=255)
                                                                                                                                                         tCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGGTGGTATAGGCT‑TCGGTG‑TATTCGCGGTTGAATTTTTCATTGTTCAGCAG‑GTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCATAGTAGTCAGCTACCGCCGCCGT‑ACGCGTAAAGCGAGga                                                                                                            >  1:382335/1‑239 (MQ=255)
                                                                                                                                                           cAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGGTGGTATAGGCT‑TCGGTG‑TATTCGCGGTTGAATTTTTCATTGTTCAGCAG‑GTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCATAGTAGTCAGCTACCGCCGCCGT‑ACGCGTAAAGCGAGggtcgatccccatc                                                                                                >  1:976725/1‑243 (MQ=255)
                                                                                                                                                              gaggaTTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGGTGGTATAGGCT‑TCGGTG‑TATTCGCGGTTGAATTTTTCATTGTTCAGCAG‑GTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCATAGTAGTCAGCTACCGCCGCCGT‑ACGCGTAAAGCGAGGATCGATCACAATCAGCtt                                                                                           >  1:821048/1‑251 (MQ=255)
                                                                                                                                                                 gaTTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGGTGGTATAGGCT‑TCGGTG‑TATTCGCGGTTGAATTTTTCATTGTTCAGCAG‑GTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCATAGTAGTCAGCTACCGCCGCCGT‑ACGCGTAAAGCGAGGATCGATACCAATCAGCTTcgc                                                                                        >  1:47515/1‑251 (MQ=255)
                                                                                                                                                                  aTTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGGTGGTATAGGCT‑TCGGTG‑TATTCGCGGTTGAATTTTTCATTGTTCAGCAG‑GTCCAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCATAGTAGTCAGCAACCGCCGCCGT‑ACGCGTAAAGCGAGGATCGATCACAATCAGCTTcgcg                                                                                       >  1:636979/1‑251 (MQ=255)
                                                                                                                                                                  aTTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGGTGGTATAGGCT‑TCGGTG‑TATTCGCGGTTGAATTTTTCATTGTTCAGCAG‑GTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCATAGTAGTCAGCTACCGCCGCCGT‑ACGCGTAAAGCGAGGATCGATCACAATCAGCTTcgcg                                                                                       >  1:219066/1‑251 (MQ=255)
                                                                                                                                                                                cGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGGTGGTATAGGCT‑TCGGTG‑TATTCGCGGTTGAATTTTTCATTGTTCAGCAG‑GTACAGCAATACGCCTGACAGGAAAGCAAGGGCAGTACCGGAACGAATAGGGGCATAGTAGTCAGCTACCGCCGCCGT‑ACGCGTAAAGCGAGGATCGATCACAATCAGCTTCGCGCCGTTGTGAAtttt                                                                         <  2:56044/251‑1 (MQ=255)
                                                                                                                                                                                 gTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGGTGGTATAGGCT‑TCGGTG‑TATTCCCGGTTGAATTTTTCATTGTTCAGCAG‑GGACAGCAATACGCCTGACAGGAAAGCAATGTCCATACCGGAACGAATAGGGGCATAGAAGGCAACTACCGCCGCCGT‑ACGCGTAAAGCGAGGATCGTTCACAATCCACTTCGCGCCGTTGGTAATTTTg                                                                        >  2:822807/1‑251 (MQ=255)
                                                                                                                                                                                   ttttCCGCGTCGTAGCCGGTGAACAGGCCCTCTTCAAAGCCGTACTCCTCACGCACGATCAGGCTGGCGGTCGTATAGGCT‑TCGGTG‑TATTCGCGGTTGAATTTTTCATTGTTCCGCAG‑GTACAGCAATACGCCTGACAGGCAAGCAATGTCAGTACCGGACCGGATAGGGGCCTAGTAGTCCGCTACCGCCCCCGTCAC‑CGACAAGCGCGGATCGCTCACACTCAGCTTCGCTCCGTGGTGAATTTTGGc                                                                      >  2:697652/1‑251 (MQ=255)
                                                                                                                                                                                   ttttCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCCGGCTGGCGGTGGTATAGGCT‑TCGGTG‑TATTCGCGGGTGAATTTTTCATTGTTCAGCAG‑GTACCGCAATACGCCTGACAGGGAAGCAATGTCCGTACCGGGACGAATAGGGGCATAGTAGACAACTACCGCCGCCGG‑ACTCGTCAAGCGAGGAGCGCTCCCAATCACCTTCGCGCCGTTGTGAAtttttgc                                                                      >  2:302759/1‑248 (MQ=255)
                                                                                                                                                                                      tCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGGTGGTATAGGCT‑TCGGTG‑TATTCGCGGTTGAATTTTTCATTGTTCAGCAG‑GTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCATAGTAGTCAGCTACCGCCGCCGT‑ACGCGTAAAGCGAGGATCGATCACAATCAGCTTCGCGCCGTTGTGAATTTTGGCtt                                                                    <  1:888704/250‑1 (MQ=255)
                                                                                                                                                                                        cgcgTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGGTGGTATAGGCT‑TCGGTG‑TATTCGCGGTTGAATTTTTCATTGTTCAGCAG‑GTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCATAGTAGTCAGCTACCGCCGCCGT‑ACGCGTAAAGCGAGGATCGATCACAATCAGCTTCGCGCCGTTGTGAATTTTGGCTTCCa                                                                 <  1:491687/251‑1 (MQ=255)
                                                                                                                                                                                          ggtcgtAGCCGGTTATTAGGCCATCTTCAAAGCCGTAATCCTCACGTCCGATCATGCTGGCGGTGGTATATGCT‑TCGGTG‑TATTCGCGGTTGAATTTTTCATTGTTCAGCAG‑GTACAGCAATACGCCTGTCAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCATTGTAGTCAGCTACCGCTGTCGT‑ACGCGTAAAGCGAGGATCGATCACAATCAGCTTCGCGCCGTTGTGAATTTTGGCTTCCATc                                                               <  1:680277/250‑1 (MQ=255)
                                                                                                                                                                                          cgtcgtAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATAAGGCTGGCGGTGGTATAGGAT‑TCGGGG‑TATTCGAGGGTGAATTTTTCATTGTTCAGAAG‑GTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCATAGTAGTAAGCTACCGCCGCCGT‑ACGCGTAAAGCGAGGGACGATCACAATCAGCATCGCGCCCTTGTGAATTTTGGCTTACATc                                                               >  1:957419/1‑251 (MQ=255)
                                                                                                                                                                                             cgtAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGGTGGTATAGGCT‑TCGGTG‑TATTCGCGGTTGAATTTTTCATTGTTCAGCAG‑GTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCATAGTAGTCAGCTACCGCCGCCGT‑ACGCGTAAAGCGAGGATCGATCACAATCAGCTTCGCGCCGTTGTGAATTTTGGCTTCCATCGcc                                                            <  1:513503/251‑1 (MQ=255)
                                                                                                                                                                                              gtAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGGTGGTATAGGCT‑TCGGTG‑TATTCGCGGTTGAATTTTTCATTGTTCAGCAG‑GTACAGCAATCCGCCTGACAGGAAAGCAATGTCAGTAACGGAACGAATAGGGGCATAGTAGTCAGCTACCGCCGCCGT‑ACGCGTAAAGCGAGGATCGATCACAATCCGCTTCGCGCCGTTGTGAATTTTGGCTTCCATCGccc                                                           >  1:955345/1‑251 (MQ=255)
                                                                                                                                                                                              gtAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGGTGGTATAGGCT‑TCGGTG‑TATTCGCGGTTGAATTTTTCATTGTTCAGCAG‑GTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCATAGTAGTCAGCTACCGCCGCCGT‑ACGCGTAAAGCGAGGATCGATCACAATCAGCTTCGCGCCGTTGTGAATTTTGGCTTCCATCGccc                                                           >  1:776880/1‑251 (MQ=255)
                                                                                                                                                                                                aGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGGTGGTATAGGCT‑TCGGTG‑TATTCGCGGTTGAATTTTTCATTGTTCAGCAG‑GTACAGCAATACGCCTGACAGGGAAGCAATGTCAGTACCGGAACGAATAGGGGCATAGTAGTCAGCTACCGCCCCCGT‑ACGCGTAAAGCGAGGGTCGATCACAATCCGCTTCGCGCCGGTGTGAATTTTGGCTTCCCTCGccc                                                           >  2:337201/1‑249 (MQ=255)
                                                                                                                                                                                                     gTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGGTGGTATAGGCT‑TGGGTG‑TATTCGCGGTTGAATTTTTCATTGTTCAGCAG‑GTAAAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCATAGTAGTCAGCTACCGCCGCCGT‑ACGCGTAAAGCGAGGATCGATCACCATCAGCTTCGCGCCGTTGTGAATTTTGGCTTCCATCGCCCAGCGGaa                                                    <  1:681724/251‑1 (MQ=255)
                                                                                                                                                                                                       gAACAGGCCGTCTTCAAAGACGTAATCATCACGCTCGATCAGGCTGGCGGTGGTATAGGCT‑TCGGTG‑TATTCGCGGTTGAATTTTTCATTGTTCAGATG‑GTACAGCATTACGCATGACAGGAAAGCAATGTCAGTACCGGAACTAATAGGGTCATAGTAGTCAGCTTCCGCCGCCGT‑ACGCGTAAAGCGAGGATCGATCACAATCAGCTTCGCGCCGTTGTGAATTTTGGCTTCCATCGCCCAGCGGAAcc                                                  <  2:653872/251‑1 (MQ=255)
                                                                                                                                                                                                       gAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGGTGGTATAGGCT‑TCGGTG‑TATTCGCGGTTGAATTTTTCATTGGTCAGCAG‑GTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCATAGTAGTCAGCTACCGCCGCCGT‑ACGCGTAAAGCGAGGATCGATCACAATCAGCTTCGCGCCGTTGTGAATTTTGGCTTCCATCGCCCAGCGGAAcc                                                  >  2:50514/1‑251 (MQ=255)
                                                                                                                                                                                                       gAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATAAGGCTGGCGGTGGTATAGGCT‑TCGGGG‑TATTCGCGGTTGAATTTTTCATTGTTCAGCAG‑GTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACAAATAGGGGCATAGTAGTCAGCTACCGCCGCCGT‑ACGCGTAAAGCGAGGATCGATCACAACCAGCTTCGCGCCGTTGTGAATTTTGGCTTCCATCGCCCAGCGGAAcc                                                  <  1:732741/251‑1 (MQ=255)
                                                                                                                                                                                                                  cTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGGTGGTATAGGCT‑TCGGTG‑TATTCGCGGTTGAATTTTTCATTGTTCAGCAG‑GTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCATAGTAGTCAGATACCGCCGCCGT‑ACGCGTAAAGCGAGGATCGATCACAAACAGCTTCGCGCCGTTGGGAGTTTTGGCTTCCATCGCCCAACGGAACCCGaaccggtgg                                       >  2:640127/1‑243 (MQ=255)
                                                                                                                                                                                                                      aaatccgttactcTCACGCACAATCAGGCTGGGGTTGTTATAGGCT‑ACGGGT‑TATGCGCGGTTAATTTTTTCATTGTGCAGCAG‑ATACAACAATACGCCTAACAGGAAAGCAATGCCAGTCCCGTAACAAAGAGGGGCATAGTAGCCAGCTACCGCCGCCGT‑ACGCGTAAAGCTAGTATCGATCACAAGCAGCTCCGCGCCGTTGTGAATTTTGGCTTCCATCGCCCAGCCGAACCCGACCGGGTGAGCta                                   <  2:80285/239‑2 (MQ=255)
                                                                                                                                                                                                                         gCCGTAATCCTCACGCACGATCAGGCTGGCGGTGGTATAGGCT‑TCGGTG‑TATTCGCGGTTGAATTTTTCATTGTTCAGCAG‑GTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCATAGTAGTCAGCTACCGCCGCCGT‑ACGCGTAAAGCGAGGATCGATCACAATCAGCTTCGCGCCGTTGTGAATTTTGGCTTCCATCGCCCAGCGGAACCCGACCGGGTGAGCTTc                                  >  1:81251/1‑249 (MQ=255)
                                                                                                                                                                                                                          ccgtattctatactgacggtcgggCTGGCGGTGGTATAGGCTATCG‑TG‑TATTCTGGTTTGAATTTTTTATGGTCCATCAGTGTA‑ATCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGTGCATATTAGTCAGCTACCGCCGCCGT‑ACGCGTAAAGCGAGGATCGATCACAATCAGCTTCGCGCCGTTGTGAATTTTGGCTTCCATCGCCCAGCGGAACCCGACCGGGTGAGCTTCAgc                               <  2:530860/229‑1 (MQ=255)
                                                                                                                                                                                                                                 ccTCACGCACGATCAGGCTGGCGGTGGTATAGGCT‑TCGGTG‑TATTCGCGGTTGAATTTTTCATTGGTCAGCAG‑GTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCATAGTAGTCAGCTACCGCCGCCGT‑ACGCGTAAAGCGAGGATCGATCACAATCAGCTTCGCGCCGTTGTGAATTTTGGCTTCCATCGCCCAGCGGAACCCGACCGGGTGAGCTTCAGCGGCGtt                         >  1:768739/1‑250 (MQ=255)
                                                                                                                                                                                                                                  cTCACGCACGATCAGGCTGGCGGTGGGATAGGCC‑TCGGGGGTAT‑CGCGGGTGCATTTTTCATTTTTCTGCAG‑GGGCAGCAATACGCCTAACAGGAAAGCAATGGCAGTACCGGAACGAATAGGGGCATAGTAGTCAGCTACCGCCGCCGT‑TCGCCTAAAGCGAGGATCGGTCACAATCAGCTTCGAGCCGTTGGGCATATTGGCTTCCCTCGCCCCGCGGAACCCGCCCGGGGGGGCGTCAGAGGCGTTAc                       >  2:783524/1‑251 (MQ=255)
                                                                                                                                                                                                                                   ccccgcccgttCAGGCTGGCGGTGTTATAGGTC‑TCGGTG‑TATTCGGGGTTGAATTTTTCATTGTTCAGCAG‑GTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCATAGTAGCCAGCTACCGCCGCCGT‑ACGCGTAAAGCGAGGATCGATCACAATCAGCTTCGCGCCGTTGTGAATTTTGGCTTCCATCGCCCAGCGGAACCCGACCGGGTGAGCTTCAGCGGCGTTAcc                      <  2:43279/241‑1 (MQ=255)
                                                                                                                                                                                                                                   ccacgcacaATCAGGCTGGCGGTGGTATAGGCT‑TCGGTG‑TATCCTCGGTGGAATTTTTCATGGTTCAGGAG‑GTACAGCAATACGCCTGACAGGAAAGCAATGTAAGTACCGGAAATAATAGGGGCATGTTAGTCAGCTTCCGCCGCCTT‑ACGCGTAAAGCTAGGATCGATCACAATCAGCTTCGCGCCGTTGTGTATTCTGGCTTCCATCGCCCAGCGGAACCCGACCGGGTGAGCTTCAGCGGCGTTAcc                      <  2:958469/250‑1 (MQ=255)
                                                                                                                                                                                                                                    cacgccctaTCAGGCTGGCGGTGGTATAGGCT‑TCGGTG‑TATTCGCGGTTGAATTTTTCATTGTTCAGCAG‑GTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCATAGTAGTCAGCTCCCGCCGCCGT‑ACGCGTAAAGCGAGGATCGATCACAATCAGCTTCGCGCCGTTGTGAATTTTGGCTTCCATCGCCCAGCGGAACCCGACCGGGTGAGCTTCAGCGGCGTTACCg                     <  2:845750/243‑1 (MQ=255)
                                                                                                                                                                                                                                     acgcacgATCAGGCTGGCGGTGGTATAGGCT‑TCGGTG‑TATTCGCGGTTGAATTTTTCATTGTTCAGCAG‑GTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCATAGTAGTCAGCTACCGCCGCCGT‑ACGCGTAAAGCGAGGATCGATCACAATCAGCTTCGCGCCGTTGTGAATTTTGGCTTCCATCGCCCAGCGGAACCCGACCGGGTGAGCTTCAGCGGCGTTACCGc                    <  1:79573/251‑1 (MQ=255)
                                                                                                                                                                                                                                       gcacgATCAGGCTGGCGGTGGTATAGGCT‑TCGGTG‑TATTCGCGGTTGAATTTTTCCTTGTTCCGCAG‑GTACAGCAATACGCCTGACCGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCATAGTAGTCAGCTACCGCCGCCGT‑ACGCGTACAGCGAGGATCGATCACAATCAGCTTCGCGCCGTTGTGAATTTTGGCTTCCATCGCCCAGCGGGACCCGACCGGGTGCGCTTCAACGGCGTTACCGccc                  >  1:92931/1‑251 (MQ=255)
                                                                                                                                                                                                                                       gcacgATCAGGCTGGCGGTGGTATAGGCT‑TCGGTG‑TATTCGCGGTTGAATTTTTCATTGTTCAGCAG‑GTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCATAGTAGTCAGCTACCGCCGCCGT‑ACGCGTAAAGCGAGGATCGATCACAATCAGCTTCGCGCCGTTGTGAATTTTGGCTTCCATCGCCCAGCGGAACCCGACCGGGTGAGCTTCAGCGGCGTTACCGccc                  >  1:655796/1‑251 (MQ=255)
                                                                                                                                                                                                                                            aTCAGGCTGGCGGTGGTATAGGCT‑TCGGTG‑TATTCGCGGTTGAATTTTTCATTGTTCAGCAG‑GTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCATAGTAGTCAGCTACCGCCGCCGT‑ACGCGTAAAGCGAGGATCGATCACAATAAGCTTCGCGCCGTTGTGAATTTTGGCTTCCATCGCCCAGCGGAACCCGACCGGGTGCGCTTCAGAGGCGTTACCGCCCATcac             >  1:975764/1‑251 (MQ=255)
                                                                                                                                                                                                                                                ggCTGGCGGTGGTATAGGCT‑TCGGTG‑TATTCGCGGTTGAATTTTTCATTGTTCAGCAG‑GTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCATAGTAGTCAGCTACCGCCGCCGT‑ACGCGTAAAGCGAGGATCGATCACAATCAGCTTCGCGCCGTTGTGAATTTTGGCTTCCATCGCCCAGCGGAACCCGACCGGGTGAGCTTCAGCGGCGTTACCGCCCATCACCacg         >  1:355734/1‑251 (MQ=255)
                                                                                                                                                                                                                                                       gTTGGTATAGGCT‑ACGGTG‑TATTTGCGGTTGAATTTTTCATTGTTCAGCAG‑GTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACTAATAGGGGCATAGTAGTCAGCTACCGCCGCCGT‑ACGCGTAAAGCGAGGAGCGATCACAATCAGCTTCGCGCCGTTGTGAATTTTGGCTTCCATCGCCCAGCGGAACCCGACCGGGTGAGCTTCAGCGGCGTTTCCGCCCATCACCACGACGAGGt  <  1:588504/251‑1 (MQ=255)
                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                             
TTTCAGGAACGCGTCTTTTGGCGTACCACAGATGTTTTCAACCACATCTGGCGTGTAGCGGGAAACGTGCTGTTTCAGCAAGTTCCACACGCAGCGCGGATGTTGCAGCGTGGTATCGCGTTTGGCGAAGCCGTTTTCGTCCAGT‑TCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGTTGGTATAGGCT‑TCGGTG‑TATTCGCGGTTGAATTTTTCATTGTTCAGCAG‑GTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCATAGTAGTCAGCTACCGCCGCCGT‑ACGCGTAAAGCGAGGATCGATCACAATCAGCTTCGCGCCGTTGTGAATTTTGGCTTCCATCGCCCAGCGGAACCCGACCGGGTGAGCTTCAGCGGCGTTACCGCCCATCACCACGACGAGGT  >  NC_000913/4084656‑4085152

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑