BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,993,531	T→C	R793R (CGT→CGC)	cyaA →	adenylate cyclase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,993,531	0	T	C	97.1%	338.6 / NA	103	R793R (CGT→CGC)	cyaA	adenylate cyclase
Reads supporting (aligned to +/- strand): ref base T (1/2); new base C (42/57); total (43/60)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

Only 100 of 104 total aligned reads displayed.

CATAACAAACTGCACGGCCTGTCAGTGCAGGTTGAAACCAATCACGTCAAATTACCGGCGGTGGTGGACGGCTTTGCCAGCGAAGGGATCATCCAGTTCTTTTTCGAAGAAACGCAAGACGAGAATGGCTTTAATATCTACATTCTCGACGAAAGCAACCGGGTTGAGGTATATCACCA‑CTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGTTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATTGTG‑AAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATCACGATACGCCGCTATTACAGCAATATTTTTCGTGATGAACGTGCCGGAAAGCGAGGCTTATCCGGCATGCAATCTTAGCGGAAACTGACTGTTTCACCCGCCTGCTGCGT  >  NC_000913/3993286‑3993774

cATAACAAACTGCACGGCCTGTCAGTGCAGGTTGAAACCAATCACGTCAAATTACCGGCGGTGGTGGGCGGCTTTGCCCGCGAAGGGGTCATCCCGGTCTTTTTTGGAGAAAAGCAAGAAGAGAATGGGTTTAATATCTACATTCTCGGCGAAAACAACCGGGTTGAGGGATATCACCA‑CTGCGGAGGCAGCAAAGAGGAGCTGGGAAGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGcttttc                                                                                                                                                                                                                                                 >  1:359519/1‑249 (MQ=255)

 aTAACAAACTGCACGGCCTGTCAGTGCAGGTTGAAACCAATCACGTCAAATTACCGGCGGTGGGGGACGGCTTTGCCAGCGAAGGGATCATCCCGTTCTTTTTCGAAGAAACGCAAGACGAGAATGGCTTTAATATCTACATTCTCGACGAAAGCAAACGGGGTGAGGGATATCACCC‑CTGCGAAGGCAGCCAAGAGGAGCTGGGACGTGACGTCAGTCGCTTCTACTCGTCATCCCCTGCCCGCTTTAcc                                                                                                                                                                                                                                                >  1:660921/1‑251 (MQ=255)

     cAAACGGCACGGCCTGTCAGGTCAGGGTGAAACCAATCACGTCAAATTTCCGGCGGTGGGGGACGGCTTTGCCAGCGAAGGGATCATCCAGTTCTTTTTCGAAGAAACGCAAGACGAGAATGGCTTTAATATCTACATTCTCGACGAAAGCAACCGGGTTGAGGTATATCACCA‑CTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGCTTTACCTACg                                                                                                                                                                                                                                            <  2:698511/251‑1 (MQ=255)

        aCTGCACGGCCTGTCAGTGCAGGTTGAAACCAATCACGTCAAATTACCGGCGGTGGTGGACGGCTTTGCCAGCGAAGGGATCATCCAGTTCTTTTTCGAAGAAACGCAAGACGAGAATGGCTTTAATATCTACATTCTCGACGAAAGCAACCGGGTTGAGGTATATCACCA‑CTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGCTTTACCTACGGCt                                                                                                                                                                                                                                         <  2:325131/251‑1 (MQ=255)

         cggcacggccgggaaggggagggtGAAACCAATCACGTCAAATTACCGGCGGTGGTGGACGGCTTTGCCAGTGAAGGGATCATCAAGTTCTTTTTCGAAGAAACGCAAGACGAGAATGGCTTTAATATCTACATTCTCGACGAAAGCAACCGGGTTGAGGTATATCACCA‑CTTCGAAGGCAGCAAAGAGGATCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGATTTACCTACGGCTc                                                                                                                                                                                                                                        <  2:530068/228‑1 (MQ=255)

            cACGGCCTGTCAGTGCAGGTTGAAACCAATCACGTCAAATTACCGGCGGTGGTGGACGGCTTTGCCAGCGAAGGGATCATCCAGTTCTTTTTCGAAGAAACGCAAGACGAGAATGGCTTTAATATCTACATTCTCGACGAAAGCAACCGGGTTGAGGTATATCACCA‑CTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGCTTTACCTACGGCTCAAg                                                                                                                                                                                                                                     <  2:189808/251‑1 (MQ=255)

            cACGGCCTGTCAGTGCAGGTTGAAACCAATCACGTCAAATTACCGGCGGTGGGGGACGGCTTTGCCAGCGAAGGGATCATCCCGGTCTTTTTTGAAGAAACGCAAAAAGCGAATGGCTTTAATATCTACATTCTCGACGAAAGCAACCGGGGTTAGGGATATCACCA‑ATTCGAAGGCAGCCAAGAGGAGCTGGGACGTGACGTCAGTCGCTTCTACTCGTCCTCCCATGAACGCTTTACCTACgggccaa                                                                                                                                                                                                                                      >  1:880558/1‑245 (MQ=255)

             ccGGCCTGTCAGTGCAGGCTGAAACCAATCACGTTAAATTACCGGCGGTGGTGGACGGCTTTGCCAGCGAAGGGATCATCCATTTCTTTTTCGAAGAAACGCAAGACGAGAATGGCTTTAATATCTACATTCTCGACGAAAGCAACCGGGTTGAGGTATATCACCA‑CTGCGAAGGCAGCAAAGAGGAGCTGGTACGTTACGTCAGTCGCGTCTACTCGTCATCGCATGACCGCTTTACCTACGGCTCAAGc                                                                                                                                                                                                                                    <  1:541146/250‑1 (MQ=255)

               ggCCTGTCAGTGCAGGTTGAAACCAATCACGTCAAATTACCGGCGGTGGTGGACGGCTTTGCCAGCGAAGGGATCATCCAGTTCTTTTTCGAAGAAACGCAAGACGAGAATGGCTTTAATATCTACATTCTCGACGAAAGCAACCGGGTTGAGGTATATCACCA‑CTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGCTTTACCTACGGCTCAAGCtt                                                                                                                                                                                                                                  <  1:551532/251‑1 (MQ=255)

               ggCCGGTCAGTGTGGGTTTAAACCAATCACGTCAAATTACCGGCGGTGGTGGACGGCTTTGCCAGCGAAGGGATCATCCAGTTCTTGTTCGAAGAAACGCAAGACGAGAATGGCTATAATATCTACATTCTCGACGAAAGCAACCGGGTTGAGGTATATCACCA‑CTTCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGCTTTACCTACGGCTCAAGCtt                                                                                                                                                                                                                                  <  2:172003/251‑1 (MQ=255)

                gCCTGTCAGTGCAGGTTGAAACCAACCACGTCAAATTACCGGCGGTGGTGGACGGCTTTGCCAGCGAAGGGATCATCCAGTTCTTTTTCGAAGAAACGCAATACGAGAATGGCTTTAATATCTCCATTCTCGACGAAAGCAACCGGGTTGAGGTATATCACCA‑CTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGCTTGACCTACGGCTCAAGCTtc                                                                                                                                                                                                                                 <  2:176380/251‑1 (MQ=255)

                    gTCAGTGCAGGTTGAAACCAATCACGTCAAATGACCGGCGGTGGTGGACGGCTTTGCCAGCGAAGGGATCATCCAGTTCTTTTTCGAAGAAACGCAAGACGAGAATGGCTTTAATATCTACATTCTCGACGAAAGCAACCGGGTTGAGGTATATCACCA‑CTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGCTTTACCTACGGCTCAAGCTtcatc                                                                                                                                                                                                                              <  1:426484/250‑1 (MQ=255)

                       aGTGCAGGTTGAAACCAATCCCGTCAAATTACCGGCGGTGGTGGACGGCTTTGCCAGCGAAGGGATCATCCAGTTCTTTTTCGAAGAAACGCAAGACGAGAATGGCTTTAATATCTACATTCTCGACGAAAGCAACCGGGTTGAGGTATATCACCA‑CTGCGAAGGCAGCAAAGAGGAGCTGTTACGTGACGTCAGTCTCTTCTACTCGTCATCGCATGACCGCTTTACCTACGGCTCAAGCTTCATCAACt                                                                                                                                                                                                                          <  1:652964/251‑1 (MQ=255)

                           cAGGTTGAAACCAATCACGTCAAATTACCGGCGGTGGTGGACGGCTTTGCCAGCGAAGGGATCATCCAGTTCTTTTTCGAAGAAACGCAAGACGAGAATGGCTTTAATATCTACATTCTCGACGAAAGCAACCGGGTTGAGGTATATCACCA‑CTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGCTTTACCTACGGCTCAAGCTTCATCAACTTCaa                                                                                                                                                                                                                      <  2:291719/251‑1 (MQ=255)

                           cAGGTTGAAACCAATCACGTCAAATTACCGGCGGTGGTGGACGGCTTTGCCAGCGAAGGGATCATCCAGTTCTTTTTCGAAGAAACGCAAGACGAGAATGGCTTTAATATCTACATGCTCGACGAAAGCAACCGGGTTGAGGTATATCACCA‑CTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGCTTTACCTACGGCTCAAGCTTCATCAACTTCaa                                                                                                                                                                                                                      <  2:771886/251‑1 (MQ=255)

                                tGAAACCAATCACGTCAAATTACCGGGGGTGGTGGACGGCTTTGCCAGCGAAGGGATCATCCAGTTCTTTTTCGAAGAAACGCAAGACGAGAATGGCTTTAATATCTACATTCTCGACGAAAGCAACCGGGTTGAGGTATATCACCA‑CTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGCTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGc                                                                                                                                                                                                                 >  2:335341/1‑251 (MQ=255)

                                 gAAACCAATCACGTCAAATTACCGGCGGTGGTGGACGGCTTTGCCAGCGAAGGGATCATCCAGTTCTTTTTCGAAGAAACGCAAGACGAGAATGGCTTTAATATCTACATTCTCGACGAAAGCAACCGGGTTGAGGTATATCACCA‑CTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGCTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGcc                                                                                                                                                                                                                <  2:149577/251‑1 (MQ=255)

                                      cAATCACGTCAAATTACCGGCGGTGGTGGACGGCTTTGCCAGCGAAGGGATCATCCAGTGCTGTTTAGAAGAAACGCAAGACGAGAATGGCGTTAATATCTACATTCTCGACGAAAGCAACCGGGTTGAGGTATATCCCCA‑CTGCGAAGGCAGCAAAGAGGAGCTGGTACGGGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGCTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGt                                                                                                                                                                                                           <  1:48037/251‑1 (MQ=255)

                                          cACGTCAAATTACCGGCGGTGGGGGACGGCTTTGCCAGCGAAGGGATCATCCAGTTCTTTTTCGAAGAAACGCAAGACGAGAATGGCTTTAATATCTACATTCTCGACGAAAGCAACCGGGTTGAGGTATATCACCC‑CTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGCTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCt                                                                                                                                                                                                        <  2:485554/250‑1 (MQ=255)

                                            cGTCAAATTACCGGCGGTGGTGGACGGCTTTGCCAGCGAAGGGATCATCCAGTTCTTTTTCGAAGAAACGCAAGACGAGAATGGCTTTAATATCTACATTCTCGACGAAAGCAACCGGGTTGAGGTATATCACCA‑CTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGCTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATc                                                                                                                                                                                                     <  2:311917/251‑1 (MQ=255)

                                               cAAATTACCGGCGGTGGTGGACGGCTTTGCCAGCGAAGGGATCATCCAGTTCTTTTTCGAAGAAACGCAAGACGAGAATGGCTTTAATATCTACATTCTCGACGAAAGCAACCGGGTTGAGGTATATCACCA‑CTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGCTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAg                                                                                                                                                                                                   <  1:183360/250‑1 (MQ=255)

                                                aaaTTTCCGGCGGTGGTGGACGGCTGTGCCAGCGAAGGGATCACCCAGTTCTGTGTCGAAGAAACGCAAGACGAGAATGGCTTTAATATCTGCATTCTCTACGAAAGCAACCGGGTTTATGTATATCACCA‑CTTCGAAGGCAGCAAAGAGGATCTGGTACGTGCCGTAAGTCGCTTCTACTCGTCATCGCATCACCTCTTGACCAACGTCTCAAGCGTCATCAACATAAACCTGCCGCAGTTCTATCAGAt                                                                                                                                                                                                 <  2:142563/251‑1 (MQ=255)

                                                         gcggTGGTGGACGGGTTTGCCAGCGAAGGGATCATCCAGTTCTTTTTCGAAGAAACGCAAGACGAGAATGGCTTTAATATCTACATTCTCGACGAAAGCAACCTGGTTGAGGTATATCACCA‑CTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGCTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATTGTG‑AAGGt                                                                                                                                                                                       <  2:416968/251‑1 (MQ=255)

                                                         gcggTGGTGGACGGCTTTGCCAGCGAAGGGATCATCCAGTTCTTTTTCGAAGAAACGCAAGACGAGAATGGCTTTAATATCTACATTCTCGACGAAAGCAACCGGGTTGAGGTATATCACCA‑CTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGCTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATTGTG‑AAGGt                                                                                                                                                                                       <  2:417000/251‑1 (MQ=255)

                                                         gcggTGGTGGACGGCTTGGCCAGCGAAGGGATCATCCATTTCTGTGTCGAAGAAACGCAAGACGAGAATGGCTTTAATATCTACATGCTCGACGAAAGCAACCGGGTTGAGGTATAGCACCA‑CTTCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCTTCATCGCATGACCGCTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCTGATTGTG‑AAGGt                                                                                                                                                                                       <  2:956947/251‑1 (MQ=255)

                                                          cggTGGTGGACGGCTTTGCCAGCGAAGGTATCATCCAGTTCTTTTTCGAAGAAACGCAAGACGAGAATGGCTTTAATATCTACATTCGCGACGAAAGCAACCGGGTTGAGGTATAGCACCA‑CTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGCTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATTGTG‑AAGGtt                                                                                                                                                                                      <  1:859242/251‑1 (MQ=255)

                                                           ggtggtGGACGGCTTTGCCAGCGAAGGGATCATCCAGTTCTTTTTCGAAGAAACGCAAGCCGAGAATGGCTTTAATATCTACATTCTCGACGACAGCAACCGGGTTGAGGTATATCCCCA‑CTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTAATCGCATGACCGCTTTACCTACGGCTCAAGCTTCATCCACTTCAACCTGCCGCAGTTCTATCAGATTGTG‑AAGGTTg                                                                                                                                                                                     >  1:564949/1‑251 (MQ=255)

                                                           ggtggtGGACGGCTTTGCCAGCGAAGGGATCATCCAGTTCTTTTTCGAAGAAACGCAAGACGAGAATGGCTTTAATATCTACATTCTCGACGAAAGCAACCGGGTTGAGGTATATCACAA‑CTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGCTTTACCTACGGCTCAAACTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATTGTG‑AAGGTTg                                                                                                                                                                                     >  2:311974/1‑251 (MQ=255)

                                                            gtggtgGACGGCTTTGCCAGCGAAGGGATCATCCAGTTCTTTTTCGAAGAAACGCAAGACGAGAATGGCTTTAATATCTACATTCTCGACGAAAGCAACCGGGTTGAGGTATATCACCA‑CTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGCTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATTGTG‑AAGGTTg                                                                                                                                                                                     >  1:232809/1‑250 (MQ=255)

                                                            gtggtgGACGGCTTTGCCAGCGAAGGGATCATCCAGTTCTTTTTCGAAGAAACGCAAGACGAGAATGGCTTTAATATCTACATTCTCGACGAAAGCAACCGGGTTGAGGTATATCACCA‑CTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGCTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATTGTG‑AAGGTTGa                                                                                                                                                                                    <  1:911784/251‑1 (MQ=255)

                                                               gtggACGGCTTTGCCAGCGAAGGGATCATCCAGTTCTTTTTCGAAGAAACGCAAGACGAGAATGGCTTTAATATCTACATTCTCGACGAAAGCAACCGGGTTGAGGTATATCACCA‑CTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGCTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATTGTG‑AAGGTTGATgg                                                                                                                                                                                 <  1:335341/251‑1 (MQ=255)

                                                                 ggACGGCTTTGCCAGCGAAGGGATCATCCAGTTCTTTTTCGAAGAAACGCAAGACGAGAATGGCTTTAATATCTACATTCTCGACGAAAGCAACCGGGTTGAGGTATATCACCA‑CTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGCTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATTGTG‑AAGGTTGATGGTc                                                                                                                                                                               <  1:706720/251‑1 (MQ=255)

                                                                 ggACGGCTTTGCCAGCGAAGGGATCATCCAGTTCTTTTTCGAAGAAACGCAAGACGAGAATGGCTTTAATATCTACATTCTCGACGAAAGCAACCGGGTTGAGGTATATCACCA‑CTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGCTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATTGTG‑AAGGTTGATGGTc                                                                                                                                                                               <  1:360788/251‑1 (MQ=255)

                                                                  gACGGCTTTGCCAGCGAAGGGATCATCCAGTTCTTTTTCGAAGAAACGCAAGACGAGAATGGCTTTAATATCTACATTCTCGACGAAAGCAACCGGGTTGAGGTATATCACCA‑CTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGCTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCCGTTCTATCAGATTGTG‑AAGGTTGATGGTCg                                                                                                                                                                              >  2:283772/1‑251 (MQ=255)

                                                                   aCGGCTGTGCCAGCGAAGTGATCATCCAGTTCTTTTTCGAAGAAACGCAAGACGAGAATGGCTTTAATATCTACATTCTCGACGAAAGCAACCGGGTTGAGGTATATCACCA‑CTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGCTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATTGTG‑AAGGTTGATGGTCGt                                                                                                                                                                             <  2:706234/251‑1 (MQ=255)

                                                                          tgacAGCGAAGGGATCATCCAGTTCTCTTTCGAAGAAACGCAAGACGAGAATGGCTTTAATATCTACATTCTCGACGAAAGCAACCGGGTTGAGGTATATCACCA‑CTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGCTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATTGTG‑AAGGTTGATGGTCGTGAACAg                                                                                                                                                                       <  2:668927/247‑1 (MQ=255)

                                                                                    gggATCATCCAGTTATTTTTCGAAGAAACGCAAGAAGAGAATGGCTTTAATATCTACAATCTCGACGAAAGCAACCGGGTTGAGGTATATC‑CCAGCTGCTAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCTCATGACCGCTTTACCTACGGCTTTAGTTTCACCAACTTCAACCTGCCGCAGTTCTATCAGATTGTG‑AAGGTTGATGGTCGTGAACAGGTGAttccgtt                                                                                                                                                            <  1:892981/251‑1 (MQ=255)

                                                                                         catcCAGTTCTTTTTCGAAGAAACGCAAGACGAGAATGGCTTTAATATCTACATTCTCGACGAAAGCAACCGGGTTGAGGTATATCACCA‑CTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGCTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATTGTG‑AAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGc                                                                                                                                                        >  1:81790/1‑250 (MQ=255)

                                                                                            ccAGTTCTTTTTCGAAGAAACGCAAGACGAGAATGGCTTTAATATCTACATTCGCGACGAAAGCAACCGGGTTGAGGTATATCACCA‑CTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGCTTTACCGACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATTGTG‑AAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGcaca                                                                                                                                                     <  1:937449/250‑1 (MQ=255)

                                                                                               gTTCTTTTTCGAAGAAACGCAAGACGACAATGGCTTTAATATCCACATTCTCGACGAAAGCAACCGGGTTGAGGTATATCACCA‑CTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATAGCATGACCGCTTTACCTACGGCTCACGCTTCATCAACTTCAACCTGCCGCCGTTCTATCAGCTTGTG‑AAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAAt                                                                                                                                                 >  1:376357/1‑251 (MQ=255)

                                                                                                     tatCGAAGAAACGAAAGACGAGAAGTGCTATAAAAGCACCAGTCACGACGAAAGCAACCAGGTTGAGGTATATCACCC‑CTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCTCTTCTACTCGTCATCGCATGTCCTCTTTACCTACGGCTCAAGCTTTATCAACTTCAACCTGCCGCAGTTCTATCAGACTGTG‑AAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTaac                                                                                                                                            <  2:28168/248‑3 (MQ=255)

                                                                                                       tCGAAGAAACGCAAGACGAGAATGGCTTTAATATCTACATTCTCGACGAAAGCAACCGGGTTGAGGTATATCACCA‑CTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGCTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTAGCAGATTGTG‑AAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCgg                                                                                                                                          <  1:901693/250‑1 (MQ=255)

                                                                                                        cGAAGAAACGCAAGACGAGAATGGCTTTAATATCTACATTCTCGACGAAAGCAACCGGGTTGAGGTATATCACCA‑CTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGCTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATTGTG‑AAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGt                                                                                                                                         <  1:133618/250‑1 (MQ=255)

                                                                                                        cGAAGAAACCCAAGACGGGAATGGCTTTAATATCTACATTCTCGACGAAAGCAACCGGGGTGAGGTATATCTCCA‑CTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGCTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATTGTG‑AAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTa                                                                                                                                        <  2:741225/251‑1 (MQ=255)

                                                                                                         gaagaaACGCAAGACGAGAATGGCTTTAATATCTACATTCTCGACGAAAGCAACCGGGTTGAGGTATATCACCA‑CTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGCTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATTGTG‑AAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTa                                                                                                                                        >  1:317753/1‑250 (MQ=255)

                                                                                                            gaaACGCAAGACGAGAATGGCTTTAATATCTACATTCCCGACGAAAGCAACCGGGTTGAGGTATATCACCA‑CTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGCTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATTGTG‑AAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACAt                                                                                                                                    <  1:570712/251‑1 (MQ=255)

                                                                                                              aaCGCAAGACGAGAACGGCTTTAATATCTACATTCTCGACGAAAGCAACCGGGTTGAGGTATATCAACA‑CTGCGAAGGCCGCAAAGAGGAGCAGGTACGTGACGTCAGCCGCTTCTACTCGTCATCGCATGACCGCTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGTTTGTG‑AAGGTTGATGGTCGTGAACAGGTCATTCCGTTCCGAACAAAATATATCGGTAACAttc                                                                                                                                  >  2:568622/1‑249 (MQ=255)

                                                                                                               aCGCAAGACGAGAATGGCTTTAATATCTACATTCTCGACGAAAGCAACCGGGTTGAGGTATATCACCA‑CTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGCTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATTGTG‑AAGGTTGATGGTCGTGCACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATgcc                                                                                                                                 >  1:565204/1‑251 (MQ=255)

                                                                                                               aCGCAAGACGAGAATGGCTTTAATATCTACATTCTCGACGAAAGCAACCGGGTTGAGGTATATCACCA‑CTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGCTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATTGTG‑AAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATgcc                                                                                                                                 >  1:103459/1‑251 (MQ=255)

                                                                                                               aCGCAAGACGAGAATGGCTTTAATATCTACATTCTCGACGAAAGCAACCGGGTTGAGGTATATCACCA‑CTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGCTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTACCAGATTGTG‑AAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATgcc                                                                                                                                 <  2:466585/251‑1 (MQ=255)

                                                                                                               aCGCAAGACGAGAATGGCTTTAATATCTACATTCTCGACGAAAGCAACCGGGTTGAGGTATATCACCA‑CTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCATCATCGCATGACCGCTTTACCTACGGCTCAATCTTCATCAACTTCAACCTGCCGCCGTTCTATCAGATTTTG‑CAGGTTGATGGTCGTGAACTGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATgcc                                                                                                                                 >  1:851100/1‑251 (MQ=255)

                                                                                                                    aGACGAGAATGGCGTTAATATCTACATTCTCGACGAAAGCAACCGGGTTGAGGTATATCACCA‑CTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGCTTTACCTACGGCTCAAGCTTCATCAACTTCCACCTGCCACTGTTCTGTCCGAT‑GTGGAAGGTTGTTGGTCATGACCAGGTGATTCCATTTCGCACAAAATCTATctcataaatggcgcc                                                                                                                              >  2:16394/1‑235 (MQ=255)

                                                                                                                                 tttAATATCTACATTCTCGACGAAAGCAACCGGGTTGAGGTATATCACCA‑CTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGCTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATTGTG‑AAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATc                                                                                                                >  1:915956/1‑250 (MQ=255)

                                                                                                                                  ttAATATCTACATGCCCGACGAAAGCAACCGGGTTGAGGTATATCACCA‑CTGCGAAGGCACCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGCTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATTGTG‑AAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATc                                                                                                                <  2:231298/249‑1 (MQ=255)

                                                                                                                                     atatCTACATTCTCGACGAAAGCAACCGGGTTGAGGTATATCACCA‑CTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGCTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATTGTG‑AAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATCACGAt                                                                                                           <  1:283772/251‑1 (MQ=255)

                                                                                                                                     atatCTACATTCCCGACGAAAGCAACCGGGTTGAGGTATATCACCA‑CTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGCTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATTGTG‑AAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATCACGAt                                                                                                           <  1:427098/251‑1 (MQ=255)

                                                                                                                                           aCATTCTCGACGAAAGCAACCGGGTTGAGGTATATCACCA‑CTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGCTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATTGTG‑AAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATCACGATAcgccg                                                                                                     <  1:570615/251‑1 (MQ=255)

                                                                                                                                           aCATTCTCGACGAAAGCAACCGGGTTGAGGTATATCACCA‑CTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCAGCGCATGACCGCTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATTGTG‑AAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATCACGATAcgc                                                                                                       <  1:691732/249‑1 (MQ=255)

                                                                                                                                               tctcGACGAAAGCAACCGGGTTGAGGTATATCACCA‑CTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGCTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATTGTG‑AAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATCACGATACGCCGCTa                                                                                                  <  1:199820/250‑1 (MQ=255)

                                                                                                                                                    acgaAAGCAACCGGGTTGAGGTATATCACCA‑CTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGCTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATTGTG‑AAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATCACGATACGCCGCTATTAc                                                                                              >  1:112339/1‑249 (MQ=255)

                                                                                                                                                       aaaGCAACCGGGTTGAGGTATATCACCA‑CTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGCTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATTGTG‑AAGGTTGATGGACGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATCACGATACGCCGCTATTACAgaa                                                                                          >  1:858271/1‑248 (MQ=255)

                                                                                                                                                       aaaGCAACCGGGTTGAGGTATATCACCA‑CTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCAACGCATGACCGCTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATTGAG‑AAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATCACGATACGCCGCTATTACAGCa                                                                                          >  2:750444/1‑250 (MQ=255)

                                                                                                                                                           cAACCGGGTTGAGGTATATCACCA‑CTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGCTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATTGTG‑AAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATCACGATACGCCGCTATTACAGCAATAtt                                                                                     >  1:536673/1‑251 (MQ=255)

                                                                                                                                                               cGGGTTGAGGTATATCACCT‑CTGCGTAGGCAGCAAAGTGGTGCTGGTACGTGGCGTCAGTCGTTTCTACTCGTCATCGCATGACCGCTTTACCTCCGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATTGGT‑AAGGTTGATGTTCGTGAACAGTTGATTCCGTTCCGCACAATATCTATCGGTAACATGACGCCTGCCAATCAGGATCACGATTCGTCGCTATTACAGCAATATTTTTc                                                                                 <  2:613034/251‑1 (MQ=255)

                                                                                                                                                               cGGGTTGAGGTATATCACCA‑CTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGCTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATTGTG‑AAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATCACGATACGCCGCTATTACAGCAATATTTTTc                                                                                 <  1:651736/251‑1 (MQ=255)

                                                                                                                                                                gggTTGAGGTATATCACCA‑CTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGCTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATTGTG‑AAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATCACGATACGCCGCTATTACAGCAATATTTTTCg                                                                                >  1:301054/1‑251 (MQ=255)

                                                                                                                                                                gggTTGAGGTATATCACCA‑CTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGGCAGTCGCTTCTACTCGTCATCGCATGACCGCTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATTGTG‑AAGGTTGATGGTCGTGAACAGGTGATTCCGTTACGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATCACGATACGCCGCTATTACAGCAATATTTTTCg                                                                                >  1:76450/1‑251 (MQ=255)

                                                                                                                                                                  gTTGAGGTATATCACCA‑CTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGCTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATTGTG‑AAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATCACGATACGCCGCTATTACAGCAATATTTTTCGtg                                                                              >  1:357503/1‑251 (MQ=255)

                                                                                                                                                                          atatCACCA‑CTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGCTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATTGTG‑AAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATCACGATACGCCGCTATTACAGCAATATTTTTCGTGATGAACg                                                                       <  1:78561/250‑1 (MQ=255)

                                                                                                                                                                             tCACCA‑CTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGCTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATTGTG‑AAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATCACGATCCGCCGCTATTACCGCAATATTTTTCGTGATGAACGGGcc                                                                   >  2:457810/1‑251 (MQ=255)

                                                                                                                                                                               ccca‑cTGCGAAGGCAGCAAAGAGGAGCGGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGCTTTACCTACGGCTCAAGCTTCATCAACTGCAACCTGCCGCAGTTCTATCAGATTGTG‑AAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATCACGATACGCCGCTATTACAGCAATATTTTTCGTGATGAACGTGCCgg                                                                 <  2:370752/250‑1 (MQ=255)

                                                                                                                                                                               acca‑cTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGCTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATTGTG‑AAGGTTGATGGTCGTGAACAGGTGAttccgttcc                                                                                                                                                          <  1:561077/162‑1 (MQ=255)

                                                                                                                                                                               acca‑cTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGCTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATTGTG‑AAGGTTGATGGTCGTGAACAGGTGAttccgttcc                                                                                                                                                          >  2:561077/1‑162 (MQ=255)

                                                                                                                                                                                     ggCGAAGGCAGCAAAGAGGAGCTGGTATGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGCTTTACCTACGGCTCAAGCTTAATCAACTTCAACCTGCCGCAGTTCTATCAGATTGTG‑AAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATCACGATACGCCGCTATTACAGCAATATTTTTCGTGATGAACGTGCCGGAAAGc                                                            <  2:660921/250‑1 (MQ=255)

                                                                                                                                                                                      gCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGCTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATTGTG‑AAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATCACGATACGCCGCTATTACAGCAATATTTTTCGTGATGAACGTGCCGGAAAGCg                                                           >  2:349642/1‑251 (MQ=255)

                                                                                                                                                                                         aaGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGCTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATTGTG‑AAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATCACGATACGCCGCTATTACAGCAATATTTTTCGTGATGAACGTGCCGGAAAGCGAgg                                                        >  1:281631/1‑251 (MQ=255)

                                                                                                                                                                                            gcagcaAGGGGGAGCTGGTACGTGTCGTCAGGCGCTTCTACTCGTCATCGCATGACCGCCTTGCCTCCGTCTCAAGCATCATCAACTTCGTCCTGCCGCAGTTCTATCAGATTGTG‑AAGGTTGATTGTCGTGAGCAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATCACGATACGCCGCGATTACAGCAACATTTTTCGTGATGAACGGGCCGTAATGCGAGGCtt                                                     <  2:575464/251‑1 (MQ=255)

                                                                                                                                                                                             cagcaAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGCTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATTGTG‑AAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATCACGATACGCCGCTATTACAGCAATATTTTTCGTGATGAACGTGCCGGAAAGCGAGGCtt                                                     >  1:15102/1‑250 (MQ=255)

                                                                                                                                                                                              agcaAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGCTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATTGTG‑AAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATCACGATACGCCGCTATTACAGCAATATTTTTCGTGATGAACGTGCCGGAAAGCGAGGCTTAt                                                   >  1:502215/1‑251 (MQ=255)

                                                                                                                                                                                               gcaAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGCTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATTGTG‑AAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATCACGATACGCCGCTATTACAGCAATATTTTTCGTGATGAACGTGCCGGAAAGCGAGGCTTATc                                                  <  2:359519/251‑1 (MQ=255)

                                                                                                                                                                                                 aaaGATGATCTGGTACGGGACGTCAGCCGTTTCTACTACTCCACGAATGGCCGTTTTAACTACGGTTCAAGTTGCATCAGATTCAACTTGCCGCAGTTCTATCAGATTGTG‑AAGGTTGAAGGTCGTGAAAAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGTCTGCCAATCTGGATCAAGATACGCCGCTATTACAGCAATATTGTTCGTTATGAACCTGCCGGAAAGCGAGGCTTATCCg                                                <  2:732641/251‑1 (MQ=255)

                                                                                                                                                                                                            ggTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGCTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATTGTG‑AAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATCACGATACGCCGCTATTACAGCAATATTTTTCGTGATGAACGTGCCGGAAAGCGAGGCTTATCCGGCATGCAATc                                      <  1:707511/250‑1 (MQ=255)

                                                                                                                                                                                                             gTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGCTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATTGTG‑AAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATCACGATACGCCGCTATTACAGCAATATTTTTCGTGATGAACGTGCCGGAAAGCGAGGCTTATCCGGCATGCAATCtt                                    >  2:416315/1‑251 (MQ=255)

                                                                                                                                                                                                               ccgttaCGTCAGTCGCTGTAAATCGTCATCGCATGCACGCGTTACCTAAGGCTCAAGCTTCATCAACTTCATCCTGCCGCAGGTCTATAAGATTGTG‑AAGGTTGATGGTCGTGAACAGTTGATTCCGTTCCGCTCAAAATCTATCGGTAACATGTCGCCTGCCAATCAGGATCACGATACGCCGCTATTACAGCAATATTTTTCGTGATGAACGTGCCGGAAAGCGAGGCTTATCCGGCATGCAATCTTAg                                  <  2:802312/246‑1 (MQ=255)

                                                                                                                                                                                                                   gACGTCAGTCGCTTCTACTCGTCATCGCATGACCGCTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATTGTG‑AAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATCACGATACGCCGCTATTACAGCAATATTTTTCGTGATGAACGTGCCGGAAAGCGAGGCTTATCCGGCATGCAATCTTAGCgg                               <  1:501223/250‑1 (MQ=255)

                                                                                                                                                                                                                     cGTCAGTCGCTTCTACTCGTCATCGCATGACCGCTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATTGTG‑AAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTA‑CATGCCGCCTGCCAATCAGGATCACGATACGCCGCTATTACAGCAATATTTTTCGTGATGAACGTGCCGGAAAGCGAGGCTTATCCGGCATGCAATCTTAGCGGaacc                           >  1:772760/1‑249 (MQ=255)

                                                                                                                                                                                                                      gTCAGTCGCTTCTACTCGTCATCGCATGACCGCTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATTGTG‑AAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATCACGATACGCCGCTATTACAGCAATATTTTTCGTGATGAACGTGCCGGAAAGCGAGGCTTATCCGGCATGCAATCTTAGCGGAAac                           <  1:374684/251‑1 (MQ=255)

                                                                                                                                                                                                                       tCAGTCGCTTCTACTCGTCATCGCATGACCGCTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATTGTG‑AAGTTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATCACGATACGCCGCTATTACAGCAATATTTTTCGTGATGAACGTGCCGGAAAGCGAGGCTTATCCGGCATGCAATCTTAGCGGAAact                          >  1:21312/1‑251 (MQ=255)

                                                                                                                                                                                                                         aGTCGCTTCTACTCGTCATCGCATGACCGCTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATTGTG‑AAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATCACGATACGCCGCTATTACAGCAATATTTTTCGTGATGAACGTGCCGGAAAGCGAGGCTTATACGGAAGTCAATCTTAGCGGAAactg                         >  2:524854/1‑250 (MQ=255)

                                                                                                                                                                                                                          gTCGCTTCTACTCGTCATCGCATGACCGTTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATTGTG‑AAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATCACGATACGCCGCTATTACAGCAATATTTTTCGTGATGAACGTGCCGGAAAGCGAGGCTTATCCGGCATGCAATCTTAGCGGAAcctgac                       >  2:393959/1‑251 (MQ=255)

                                                                                                                                                                                                                           tCGCTTCTACTCGTCATCGCATGACCGCTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATTGTG‑AAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATCACGATACGCCGCTATTACAGCAATATTTTTCGTGATGAACGTGCCGGAAAGCGAGGCTTATCCGGCATGCAATCTTAGCGGAAactgact                      >  2:895466/1‑251 (MQ=255)

                                                                                                                                                                                                                            cGCTTCTACTCGTCATCGCATGACCGCTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATTGTG‑AAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATCACGATACGCCGCTATTACAGCAATATTTTTCGTGATGAACGTGCCGGAAAGCGAGGCTTATCCGGCATGCAATCTTAGCGGAAactgactg                     <  1:649936/251‑1 (MQ=255)

                                                                                                                                                                                                                            cGCTTCTACTCGTCATCGCATGACCGCTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATTGAG‑CAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTACCATGCCGCCTGCCAATCAGGATCACGATACGCCGCTATTACAGCAATATTTTTCGTGATGAACGTGCCGGAAAGCGAGGCTTATCCGGAATACAATATTACCGGCAactgaccg                     >  1:932825/1‑249 (MQ=255)

                                                                                                                                                                                                                            cGCTCCTACTCGTCACCGCATGACCGCTTTACCTACGGCTCAAGCTTCATCAACTGCAACCTGCCGCAGTTCTATCAGATTGTG‑AAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATCACGATACGCCGCTATTACAGCAATATTTTTCGTGATGAACGTGCCGGAAAGCGAGGCTTATCCGGCATGCAATCTTAGCGGAAactgact                      <  2:162835/250‑1 (MQ=255)

                                                                                                                                                                                                                               ttCTACTCGTCATCGCATGACCGCTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATTGTG‑AAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATCACGATACGCCGCTATTACAGCAATATTTTTCGTGATGAACGTGCCGGAAAGCGAGGCTTATCCGGCATGCAATCTTAGCGGAAACTGACTGttt                  >  1:178390/1‑251 (MQ=255)

                                                                                                                                                                                                                                    cTCGTCATCGCATGACCGCTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATTGTG‑AAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATCACGATACGCCGCTATTACAGCAATATTTTTCGTGATGAACGTGCCGGAAAGCGAGGCTTATCCGGCCTGCAATCTTAGCGGAAACTGACTGTTTCAccc             >  2:767741/1‑251 (MQ=255)

                                                                                                                                                                                                                                      cGTCATCGCATGACCGCTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATTGTG‑AAGGTTGCTGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATCACGATACGCCGCTATTACAGCAATATTTTTCGTGATGAACGTGCCGGAAAGCGAGGCTTATCCGGCATGCAATCTTAGCGGAAACTGACTGATTCAACCGc           >  1:131565/1‑251 (MQ=255)

                                                                                                                                                                                                                                       gTCATCGCATGACCGCTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATTGTG‑AAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATCACGATACGCCGCTATTACAGCAATATTTTTCGTGATGAACGTGCCGGAAAGCGAGGCTTATCCGGCATGCAATCTTAGCGGAAACTGACTGTTTCACCCGcc          >  1:298191/1‑251 (MQ=255)

                                                                                                                                                                                                                                         cagcGCATGACCGTTTTACCTACGTCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATTGTG‑AAGGTTGATGGTAGTGAACAGTTGATTCCGTTCCGCACAAAATCTATCGGTATCATGCCGCCTGCCAATCAGGATCACGATACGCCGCTATTACAGCAATATTTTTCGTGATGAACGTGCCGGAAAGCGAGGCTTATCCGGAATGCAATCTTAGCGGAAACTGACTGTTTCACCCGCctg        <  1:444759/248‑1 (MQ=255)

                                                                                                                                                                                                                                               aTGACCGCTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATTGTG‑AAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATCACGATACGCCGCTATTACAGCAATATTTTTCGTGATGAACGTGCCGGAAAGCGAGGCTTATCCGGCATGCAATCTTAGCGGAAACTGACTGTTTCACCCGCCTGCTGCGt  >  1:293933/1‑251 (MQ=255)

CATAACAAACTGCACGGCCTGTCAGTGCAGGTTGAAACCAATCACGTCAAATTACCGGCGGTGGTGGACGGCTTTGCCAGCGAAGGGATCATCCAGTTCTTTTTCGAAGAAACGCAAGACGAGAATGGCTTTAATATCTACATTCTCGACGAAAGCAACCGGGTTGAGGTATATCACCA‑CTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCAGTCGCTTCTACTCGTCATCGCATGACCGTTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATTGTG‑AAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATCACGATACGCCGCTATTACAGCAATATTTTTCGTGATGAACGTGCCGGAAAGCGAGGCTTATCCGGCATGCAATCTTAGCGGAAACTGACTGTTTCACCCGCCTGCTGCGT  >  NC_000913/3993286‑3993774

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`