Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,823,162 C→T H255Y (CAC→TAC)  spoT → bifunctional (p)ppGpp synthase/hydrolase SpoT

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,823,1620CT95.2% 318.1 / 3.8 105H255Y (CAC→TAC) spoTbifunctional (p)ppGpp synthase/hydrolase SpoT
Reads supporting (aligned to +/- strand):  ref base C (2/3);  new base T (52/47);  total (54/51)
Fisher's exact test for biased strand distribution p-value = 6.70e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.52e-01

Only 100 of 105 total aligned reads displayed.

TTAGGTATCCACCACATTAAAACCGAACTCGAAGAGCTGGGTTTTGAGGCGCTGTATCCCAACCGTTATCGCGTAATCAAAGAAGTGGTGAAAGCCGCGCGCGGCAACCGTAAA‑GAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACT‑GCAAAATGGTGCTCAAAGAGCAGCGTTTT‑CACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGACCAGATGGCGGAGATGGGTGTT  >  NC_000913/3822916‑3823404
                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                  
ttAGGTATCCACCACATTAAAACCGAACTCGAAGAGCTGGGTTTTGAGGCGCTGTATCCCAACCGTTATCGCGTAATCAAAGAAGTGGTGAAAGCCGCGCGCGGCAACCGTAAA‑GAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACT‑GCAAAATGGTGCTCAAAGAGCAGCGTTTT‑TACt                                                                                                                                                                                                                                                 <  1:957629/250‑1 (MQ=255)
           ccacATTAAAACCGAACTCGACGAGCTGGGTTTTGAGGCGCTGTATCCCAACCGTTATCGCGTAATCAAAGAAGTGGTGAAAGCCGCGCGCGGCAACCGTAAA‑GAGATGATCCAGAAGATTCTTTCTGAAATCGCAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACT‑GCAAAATGGTGCTCAAAGAGCAGCGTTTT‑TACTCGATCATGGAc                                                                                                                                                                                                                                      >  2:875034/1‑250 (MQ=255)
               aTTAAAACCGAACTCGAAGAGCTGGGTTTTGAGGCGCTGTATCCCAACCGTTATCGCGTAATCAAAGAAGTGGTGAAAGCCGCGCGCGGCAACCGTAAA‑GAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACT‑GCAAAATGGTGCTCAAAGAGCAGCGTTTT‑TACTCGATCATGGACATCt                                                                                                                                                                                                                                  >  1:324455/1‑250 (MQ=255)
               aTTAAAACCGAACTCGAAGAGCTGGGTTTTGAGGCGCTGTATCCCAACCGTTATCGCGTAATCAAAGAAGTGGTGAAAGCCGCGCGCGGCAACCGTAAA‑GAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACT‑GCAAAATGGTGCTCAAAGAGCAGCGTTTT‑TACTCGATCATGGACATCTa                                                                                                                                                                                                                                 <  1:226509/251‑1 (MQ=255)
                 tAAAACCGAACTCGAAGAGCTGGGTTTTGAGGCGCTGTATCCCAACCGTTATCGCGTAATCAAAGAAGTGGTGAAAGCCGCGCGCGGCAACCGTAAA‑GAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACT‑GCAAAATGGTGCTCAAAGAGCAGCGTTTT‑TACTCGATCATGGACATCTAc                                                                                                                                                                                                                                <  2:612393/250‑1 (MQ=255)
                 tAAAACCGAACTCGAAGAGCGGGGTTTTGAGGCGCTGTATCCCAACCGTTATCGCGTAATCAAAGAAGTGGTGAAAGCCGCGCGCGGCAACCGTAAA‑GAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACT‑GCAAAATGGTGCTCAAAGAGCAGCGTTTT‑CACTCGATCATGGACATCTAc                                                                                                                                                                                                                                <  2:68646/250‑1 (MQ=255)
                    aaCCGAACTCGAAGCGCTGGGTTTTGAGGCGCTGTATCCCAACCGTTATCGCGTAATCAAAGAAGTGGTGAAAGCCGCGCGCGGCAACCGTAAA‑GAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACT‑GCAAAATGGTGCTCAAAGAGCAGCGTTTT‑TACTCGATCATGGACATCTACGCtt                                                                                                                                                                                                                            >  1:777617/1‑251 (MQ=255)
                       cGAACTCTAAGAGCTGGGTTTTGAGGCGCTGTATCCCAACCGTTATCGCGTAATCAAAGAAGTGGTGAAAGCCGCGCGCGGCAACCGTAAA‑GAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACT‑GCAAAATGGTGCTCAAAGAGCAGCGTTTT‑TACTCGATCATGGACATCTACGCTTTcc                                                                                                                                                                                                                         <  1:873582/251‑1 (MQ=255)
                            tCGAAGAGCTGGGTTTTGAGGCGCTGTATCCCAACCGTTATCGCGTAATCAAAGAAGTGGTGAAAGCCGCGCGCGGCTACCGTAAA‑GAGATGAACCAGAGGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGACGCGTCAGTGGTCGCGCGACGCATCTTTATTCGATTTACT‑GCAAAATGGTGCTCAAAGAGCAGCGTTTT‑TACTCGATCGTGGACATCTACGCTTTCCACGTg                                                                                                                                                                                                                    >  2:677260/1‑251 (MQ=255)
                              gAAGAGCTGTGTTTTGAGGCGCTGTATCCCAACCGTTATCGCGTAATCAAAGAAGTGGTGAAAGCCGCGCGCGGCAACCGTAAA‑GAGATGATCCAGAAGATTCGTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACT‑GCAAAATGGTGCTCAAAGAGCAGCGTTTT‑TACTCGATCATGGACATCTACGCTTTCCGCGTGAt                                                                                                                                                                                                                  <  2:143860/251‑1 (MQ=255)
                              gAAGAGCTGGGTTTTGAGGCGCTGTATCCCAACCGTTATCGCGTAATCAAAGAAGTGGTGAAAGCCGCGCGCGGCAACCGTAAA‑GAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACT‑GCAAAATGGTGCTCAAAGAGCAGCGTTTT‑TACTCGATCATGGACATCTACGCTTTCCGCGTGAt                                                                                                                                                                                                                  <  1:45050/251‑1 (MQ=255)
                              gAAGAGCTGGGTTTTGAGGCGCTGTATCCCAACCGTTATCGCGTAATCAAAGAAGTGGTGAAAGCCGCGCGCGGCAACCGTAAA‑GAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACT‑GCAAAATGGTGCTCAAAGAGCAGCGTTTT‑TACTCGATCATGGACATCTACGCTTTCCGCGTGAt                                                                                                                                                                                                                  >  1:705117/1‑251 (MQ=255)
                                   gcttggTTTTGTAGCGCTGAATCCCAACCGTTATCGCGTAATCAAAGAAGGGGTGACAGCCGCGCGCGGCA‑CCATAAAAGAGATGATCCAGAAGGTACTGTCTGAAATCGAAGGGCGTATACAGGACGCGAGAATACCGTGACGCGTCAGTGGTCGCGCGAAGCATCTTTATTCGATTTACT‑GCAAAATGGGTCTCAAAGCGCAGCGTTTT‑TACTCTATCATCGCCATCTaagattacctcgtgcttgtc                                                                                                                                                                                                              >  2:851151/5‑231 (MQ=255)
                                   gCTGGGTTTTGAGGCGCTGTATCCCAACCGTTATCGCGTAATCAAAGAAGTGGTGAAAGCCGCGCGCGGCAACCGTAAA‑GAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATAACGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACT‑GCAAAATGGTGCTCAAAGAGCAGCGTTTT‑TACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTc                                                                                                                                                                                                              >  1:102879/1‑250 (MQ=255)
                                       ggTTTTGAGGCGCTGTATCCCAACCGTTATCGCGTAATCAAAGAAGTGGTGAAAGCCGCGCGCGGCAACCGTAAA‑GAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACT‑GCAAAATGGTGCTCAAAGAGCAGCGTTTT‑TACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGa                                                                                                                                                                                                         <  1:638456/251‑1 (MQ=255)
                                        gttgtGAGGCTCTGTCACCCAACCGTTCACGCGTAATCAAAGTAGTGGGGAAAGCCGCGCGCGGCAACCGTAAA‑GAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGGTTGCCGGCAGCGGTAATACCGTGTCGTGGCAGTGGTCGCGAGAAGCATCTTTATTCGATCTACT‑GCAAAATGGTTCTCAAAGAGCAGCGTTTT‑TACTCGATCATGGACATCTACGCTTGACGCGTGATCTTCAATGAt                                                                                                                                                                                                        <  2:836677/247‑1 (MQ=255)
                                          tttGAGGCGCTGTATCCCAACCGTTATCGCGTAAACAAAGAAGTGGTGAAAGCCGCGCGCGGCAACCGTAAA‑GAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGTGAAGCATCTTTATTCGATTTACT‑GCAAAATGGTGCTCAAAGAGCAGCGTTTT‑TACTCGATCATGGACATCTACGCTTGCCGCGTGATCGTCAATGAtt                                                                                                                                                                                                       <  2:279915/250‑1 (MQ=255)
                                            tggggCGCTGTCTCCCAACCGTTACCGGGTAATCAAAGAAGTGGTGAAAGCCGCGCGCGGCAACCGTAAA‑GAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACT‑GCAAAATGGTGCTCAAAGAGCAGCGTTTT‑TACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCt                                                                                                                                                                                                     <  2:795428/247‑1 (MQ=255)
                                             gAGGCGCTGTATCCCAACCGTTATCGCGTAATCAAAGAAGTGGTGAAAGCCGCGCGCGGCAACCGTAAA‑GAGAAGAACCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGCGGTCGCGAGAAGCATCTTTATTCGATTTACT‑GCAAAGTGGTGCTCAAAGAGCAGCGTTTT‑TACTCGTTCATGGACCTCGCCGCTTTACGCGTGATCGTCAATGATTCTg                                                                                                                                                                                                    >  2:567144/1‑250 (MQ=255)
                                              atgctcTGTATCCCACCCGTTATCGCGTAGTCCAAGAAGGGGTGAAAGCCGCCCCCGGCAACCGTAAA‑GAGCTGATCCAGGAGCTTCTTTCTGAAATCGTAGGGCGTTTGCGGGAAGCGGGAATACCGGGCCGCGTAAGTGGTCGCGAGAAGCATCTTTATTCGATTTAAT‑GCAAAATGGTGCTCAAAAAGCAGCGATTT‑TACTCGATCATGGACATCTAAGCTTTCCGCGTGATCGTCAATTATTGTGac                                                                                                                                                                                                  >  2:367590/6‑251 (MQ=255)
                                                  gcTGTAGCCCAACCGTTATCGAGTAATAAAAGAAGTGGGGAAAGCCGCGCGCGGCAACCGTAAA‑GAGAGGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGGTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACT‑GCAAAATGGTGCTCAAAGAGCAGCGTTTT‑TACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCt                                                                                                                                                                                              <  2:33214/251‑1 (MQ=255)
                                                    tGTATCCCAACCGTTATCGCGTAATCAAAGAAGGGGTGAAAGCCGCGCGCGGCAACCGTAAA‑GAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACT‑GCAAAATGGTGCTCAAAGAGCAGCGTTTT‑TACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGt                                                                                                                                                                                            >  1:672688/1‑251 (MQ=255)
                                                     gTATCCCAACCGTTATCGCGTAATCAAAGAAGTGGTGAAAGCCGCGCGCGGCAACCGTAAA‑GAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACT‑GCAAAATGGTGCTCAAAGAGCAGCGTTTT‑TACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGtt                                                                                                                                                                                           <  1:949698/251‑1 (MQ=255)
                                                        tCCCAACCGGTATCGCGTAATCAAAGAAGTGGTGAAAGCCGCGCGCGGCAACCGTAAA‑GAGATGATCCAGAAGATTCGTTCTGAAATCGAAGGGCGGTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACT‑GCAAAATGGTGCTCAAAGAGCAGCGTTTT‑TACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTAt                                                                                                                                                                                         <  2:550861/250‑1 (MQ=255)
                                                          ccAACCGTTATAGCGTAATCAAAGAAGTGGTGAAAGCCGCGCGCGGCACCCGTAAA‑GAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACT‑GCAAAATGGTGCTCAAAAAGCAGCGTTTT‑TACTCGATCATGGCCATCTACGCTTTCCGCGTGATCGTCAATGGTTCTGACACCTGTTATcgc                                                                                                                                                                                      >  2:561011/1‑251 (MQ=255)
                                                           ccaCCGTTATCGCGTAATCAAAGAAGTGGTGAAAGCCGCGCGCGGCAATCGTAAA‑GAGATGATCCAGAATATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACT‑GCAAAATGGTGCTCAAAGAGCAGCGTTTT‑TACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATcgcg                                                                                                                                                                                     <  2:755511/249‑1 (MQ=255)
                                                           cAACCGTTATCGCGTAATCAAAGAAGTGGTGAAAGCCGCGCGCGGCAACCGTAAA‑GAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGACGAGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACT‑GCAAAATGGTGACCAAAGAGCAGCGTTTTTCA‑TCGATCATGGAAATCTACGCTTTCCGAGGGATCGACAATGATTCTGACACCTGTTATcgcg                                                                                                                                                                                     >  2:514753/1‑251 (MQ=255)
                                                             aCCGTTATCGCGTAATCAAAGAAGTGGTGAAAGCCGCGCGCGGCAACCGTAAA‑GAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACT‑GCAAAATGGTGCTCAAAGAGCAGCGTTTT‑TACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTg                                                                                                                                                                                   <  1:354667/251‑1 (MQ=255)
                                                                gTTATCGCGTAATCAAAGAAGTGGTGAAAGCCGCGCGCGGCAACCGTAAA‑GAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACT‑GCAAAATGGTGCTCAAAGAGCAGCGTTTT‑TACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTg                                                                                                                                                                                >  1:483796/1‑251 (MQ=255)
                                                                     cgcgTAATCAAAGAAGTGGTGAAAGCCGCGCGCGGCAACCGTAAA‑GAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACT‑GCAAAATGGTGCTCAAAGAGCAGCGTTTT‑CACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCa                                                                                                                                                                           <  1:216986/251‑1 (MQ=255)
                                                                             cAAAGAAGTGGTGAAAGCCGCGCGCGGCAACCGTAAA‑GAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACT‑GCAAAATGGTGCTCAAAGAGCAGCGTTTT‑TACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGcac                                                                                                                                                                    >  1:575726/1‑250 (MQ=255)
                                                                              aaaGAAGTGGTGAAAGCCGCGCGCGGCAACCGTAAA‑GAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACT‑GCAAAATGGTGCTCAAAGAGCAGCGTTTT‑TACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGcac                                                                                                                                                                    >  1:694271/1‑249 (MQ=255)
                                                                              aaaGAAGTGGTGAAAGCCGCGCGCGGCAACCGTAAA‑GAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACT‑GCAAAATGGTGCTCAAAGAGCAGCGTTTT‑TACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAg                                                                                                                                                                  <  1:222313/251‑1 (MQ=255)
                                                                                  aagTGGTGAAAGCCGCGCGCGGCAACCGTAAA‑GAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACT‑GCAAAATGGTGCTCAAAGAGCAGCGTTTT‑TACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTg                                                                                                                                                              <  1:731036/251‑1 (MQ=255)
                                                                                   agTGGTGAAAGCCGCTCGCGGCAACCGTAAA‑GAGATGATCCAGAAGATTCTTTCTGAAATCTAAGGGCGTGTGCATGAAGCGGGAATACCGTGCCGCGTCAGGGGTCGCGATAAGCATCTTTATTCGATTTACT‑GCAAAATGGTGCTCAAAGATCATCGTTTT‑GACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCTTTCTGGGCCAGATGCACAGCCTGt                                                                                                                                                             <  2:494044/251‑1 (MQ=255)
                                                                                       gtgAAAGCCGCGCGCGGCAACCGTAAA‑GAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACT‑GCAAAATGGTGCTCAAAGAGCAGCGTTTT‑TACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACa                                                                                                                                                          >  1:34569/1‑250 (MQ=255)
                                                                                         gAAAGCCGCGCGCGGCAACCGTAAA‑GAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTGTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACT‑GCAAAATGGTGCTCAAAGAGCAGCGTTTT‑TACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGc                                                                                                                                                       <  1:784583/251‑1 (MQ=255)
                                                                                          aaaGCCGGGCGCGGCAACCGTAAA‑GAGATGATCCAGAAGATTCTTTCGGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACT‑GCAAAATGGTGCTCAAAGAGCAGCGTTTT‑TACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGcc                                                                                                                                                      <  2:34569/251‑1 (MQ=255)
                                                                                                 cgcgcgGCAACCGTAAA‑GAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGGGCCGCGTCAGTGGGCGCGAGAAGCATCTTTATTCGATTTACT‑GCAAAATGGTGCTCAAAGAGCAGCGTTTT‑TACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCg                                                                                                                                               <  2:733340/251‑1 (MQ=255)
                                                                                                      ggCAACCGTAAA‑GAGATGAGCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACT‑GCAAAATGGTGCTCAAAGAGCATCGTTTT‑TACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCGGTGATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCcg                                                                                                                                          <  1:65124/251‑1 (MQ=255)
                                                                                                          aCCGTAAA‑GAGATGATCCAGAAGATGCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAAGCCCGTGCCGCGTCAGTGGTCGCGAGACGCATCGTTATTCGAGTTACT‑GCAAAATGGTGCCCAAAGAGCAGCGTTTT‑TACTCGAGCATGGACATCTACTCTTTCCGCTTGATCTTCAATGATTCTGACACCTGTTATCGCTTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTg                                                                                                                                      <  1:618375/251‑1 (MQ=255)
                                                                                                           ccGTAAA‑GAGATGATCCAGAAGATTCTTTCTGAAACCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACT‑GCAAAATGGTGCTCAAAGAGCAGCGTTTT‑TACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGa                                                                                                                                     <  2:47768/251‑1 (MQ=255)
                                                                                                                    agaTGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACT‑GCAAAATGGTGCTCAAAGAGCAGCGTTTT‑TACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACtat                                                                                                                             >  1:909427/1‑251 (MQ=255)
                                                                                                                        gatCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACT‑GCAAAATGGTGCTCAAAGAGCAGCGTTTT‑TACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCg                                                                                                                         <  1:983339/251‑1 (MQ=255)
                                                                                                                            cAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACT‑GCAAAATGGTGCTCAAAGAGCAGCGTTTT‑TACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACATGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCAt                                                                                                                     >  2:833231/1‑251 (MQ=255)
                                                                                                                            cAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGAGGTCGCGAGAAGCATCTTTATTCGATTTACT‑GCAAAATGGTGCTCAAAGAGCAGCGTTTT‑TACTCGATCATGGACATCTAAGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGACAGACTATATCGccct                                                                                                                     >  2:877151/1‑249 (MQ=255)
                                                                                                                               aagaTTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACT‑GCAAAATGGTGCTCAAAGAGCAGCGTTTT‑TACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTcc                                                                                                                  >  1:16713/1‑251 (MQ=255)
                                                                                                                               aagaTTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACT‑GCAAAATGGTGCTCAAAGAGCAGCGTTTT‑TACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACATGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTcc                                                                                                                  >  1:668202/1‑251 (MQ=255)
                                                                                                                                       ttcggAAATCGAAGGGGGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTTGTCGCGAGAAGCATCTTTATTCGATTTACT‑GCAAAATGGTGCTCAAAGAGCAGCGTTTT‑TACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTTTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGc                                                                                                            <  2:293560/245‑1 (MQ=255)
                                                                                                                                       tgtgaaaaTCGAATGGTGGTTGTAGGAAGCGGGAATACCGGGCCGCGGCAGTGGGCGCGAGAAGCATCTTTATTCGATTTACT‑GCAAAATGGGGCTCAAAGGGCAGCGTTTT‑TACTCGATCATGGACATCTCCGCTTTCCGCGTTAGCGTCAATGATTCTGACCCCTGTTACCGCGTGCTGGGCCAGATGCACAGCCTGTACGAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCg                                                                                                           <  2:581133/245‑1 (MQ=255)
                                                                                                                                         ctGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACT‑GCAAAATGGTGCTCAAAGAGCAGCGTTTT‑TACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAAc                                                                                                        >  1:507388/1‑251 (MQ=255)
                                                                                                                                          tGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACT‑GCAAAATGGTGCTCAAAGAGCAGCGTTTT‑TACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACg                                                                                                       >  1:151034/1‑251 (MQ=255)
                                                                                                                                           gAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACT‑GCAAAATGGTGCTCAAAGAGCAGCGTTTT‑TACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACgg                                                                                                      >  1:570698/1‑251 (MQ=255)
                                                                                                                                           gAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACT‑GCAAAATGGTGCTCAAAGAGCAGCGTTTT‑TACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACgg                                                                                                      >  1:450079/1‑251 (MQ=255)
                                                                                                                                           gAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACT‑GCAAAATGGTGCTCAAAGAGCAGCGTTTT‑TACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACg                                                                                                       <  1:297945/250‑1 (MQ=255)
                                                                                                                                           gAAATCGAAGGGCGGTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACT‑GCAAAATGGTGCTCAAAGAGCAGCGTTTT‑TACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCGGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACg                                                                                                       <  1:590626/250‑1 (MQ=255)
                                                                                                                                            aaaTCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACT‑GCAAAATGGTGCTCAAAGAGCAGCGTTTT‑TACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGc                                                                                                     <  1:38733/251‑1 (MQ=255)
                                                                                                                                                  aacggCGTTTGCAGGAAGCTGGAGTACCGTGCCTCGTCAGTGGTCGCGAGAAGCATCTTTTTGCGATTTACG‑GCAAAATGGTGCTCAAAGAGCAGCGTTTT‑TACTCTATCATGTACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACTCCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGTCTATATCGCCATTCCTAAAGCGAACGGCTATCAg                                                                                               <  1:546566/248‑1 (MQ=255)
                                                                                                                                                  aaGGGCATTTGCAGGAAGCGGGAAGCCCGTGCCGAGTCAGGGGTCTCGAGAAGCAGCTTTATTCGATTTACT‑GCAAAATTGTGCTCAAATAGCCGCGTTTT‑TACTCTATCATGGACATCTCCGCTTTCCGCGGGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCTCAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAg                                                                                               <  2:766674/251‑1 (MQ=255)
                                                                                                                                                       cGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACT‑GCAAAATGGTGCTCAAAGAGCAGCGTTTT‑TACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCttt                                                                                          >  2:331693/1‑251 (MQ=255)
                                                                                                                                                        gTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACT‑GCAAAATGGTGCTCAAAGAGCAGCGTTTT‑TACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCCGTCTTTg                                                                                         >  2:307260/1‑251 (MQ=255)
                                                                                                                                                        gTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACT‑GCAAAATGGTGCTCAAAGAGCAGCGTTTT‑TACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGAGGCACAGCCTGTACAAGCCGCGGCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTg                                                                                         <  1:112342/251‑1 (MQ=255)
                                                                                                                                                        gTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACT‑GCAAAATGGTGCTCAAAGAGCAGCGTTTT‑CACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGaaa                                                                                                                                   <  1:125873/209‑1 (MQ=255)
                                                                                                                                                        gTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACT‑GCAAAATGGTGCTCAAAGAGCAGCGTTTT‑CACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGaaa                                                                                                                                   >  2:125873/1‑209 (MQ=255)
                                                                                                                                                             aaGGAAGCGGGAATACCGTGCCGCGCCAGGGGTCGCGAGAAACATCTTTATTCGATTTACT‑GAAAAATGGTGCTCAAAGAGCAGCGGTTT‑TACCAGATCATGGCCATCTACGCTTTCCGCGGTCTCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGTTGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTGGCacac                                                                                    <  2:745747/250‑1 (MQ=255)
                                                                                                                                                              aGGAAGCGGGAATACAGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACT‑GCAAAATGGTGCTCAAAGAGCAGCGTTTT‑TACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGCCTATATCGCCATTCCCAAAGCGAACGGCTATCAGTCTTTGCACAcc                                                                                   >  2:214127/1‑251 (MQ=255)
                                                                                                                                                               ggAAGCGGGAATACCGTGGCGGGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACT‑GAAAAATGGTGCTAAAAGAGCAGCGTTTT‑TACTCGATCATGGACATATACGCTTTCCGCGTGATCGTCAAGGATTCTGACCCCTGTTATCGCGTGCTGGGCCAGATGCAAAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCt                                                                                  <  2:345573/251‑1 (MQ=255)
                                                                                                                                                                 aaGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATGCGATTTACT‑GCAAAATGGTGCTCAAAGAGCAGCGTTTT‑TACTCGATCATGGACATCTACGCTTTCCGCGTGAGCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCg                                                                                <  2:186174/251‑1 (MQ=255)
                                                                                                                                                                      ggAATACCGTTCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACT‑GCAAAATGGTGCTCAAAGAGCAGCGTTTT‑TACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCgatgat                                                                           <  1:177251/251‑1 (MQ=255)
                                                                                                                                                                            cctggccgcttgaGTGGTCGCTAGAAGCATCTTTACTCGTTTTACT‑GCAAAATTGGGCTCAAAGAGCAGCTTTTT‑TACTCGGTCATTGGTTTCTGCGCTTTCCGCGTGAGCTTCATTGAGTCTGCCCCCTGTCAGCGCGTGCTGGGCCAGATGCACAGCTTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGTCTATCAGTCTTTGCACCCCTCGATGATCGGctc                                                                     <  2:802089/239‑3 (MQ=255)
                                                                                                                                                                                      tCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACT‑GCAAAATGGTGCTCAAAGAGCAGCGTTTT‑TACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGaa                                                                                                         >  2:126033/1‑205 (MQ=255)
                                                                                                                                                                                      tCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACT‑GCAAAATGGTGCTCAAAGAGCAGCGTTTT‑TACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGaa                                                                                                         <  1:126033/205‑1 (MQ=255)
                                                                                                                                                                                         ggggTCGCGAGAAGCATCTTTATTCGATTTACT‑GCAAAATGGTGCTCAAAGAGCAGCGTTTT‑TACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCg                                                        <  1:523919/249‑1 (MQ=255)
                                                                                                                                                                                         gTGGTCGCGAGAAGCATCTTTATTCGATTTACT‑GCAAAATGGTGCTCAAAGAGCAGCGTTTT‑TACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGTTCGGCCCGCACGGTGTGCCg                                                        >  1:77833/1‑251 (MQ=255)
                                                                                                                                                                                                cgAGAAGCATCTTTATTCGATTTACT‑GCAAAATGGTGCTCAAAGAGCAGCGTTTT‑TACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGCTTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAACAGCGAACGGCTATAAGTCTTTGCACCCCTCGATGATCGGCCCGCACGGTGTGACGGTTGAgg                                                 >  2:922477/1‑251 (MQ=255)
                                                                                                                                                                                                   gaAGCATCTTTATTCGATTTACT‑GCAAAATGGTGCTCAAAGAGCAGCGTTTT‑TACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTcc                                              >  2:100404/1‑251 (MQ=255)
                                                                                                                                                                                                   aaaGCATCTTTATTCGATTTACT‑GCAAAATGGTGCTCAAAGAGCAGCGTTTT‑TACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTcc                                              <  1:30974/250‑1 (MQ=255)
                                                                                                                                                                                                     aGCATCTTTATTCGATTTACT‑GCAAAATGGTGCTCAAAGAGCAGCGTTTT‑TACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCAAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAg                                            <  1:325814/251‑1 (MQ=255)
                                                                                                                                                                                                      gCATCTTTATCCGATTTACT‑GCAAAATGGTGCTCAAAGAGCAGCGTTTT‑TACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTCCAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGa                                           <  2:327600/251‑1 (MQ=255)
                                                                                                                                                                                                          cTTTATTCGATTTACT‑GCAAAATGGTGCTCAAAGAGCAGCGTTTT‑TACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGCTGCACAGCCTGTACACGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCCTTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCg                                       >  2:873329/1‑251 (MQ=255)
                                                                                                                                                                                                          cTTTATTCGATTTACT‑GCAAAATGGTGCTCAAAGAGCAGCGTTTT‑TACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCg                                       >  1:80039/1‑251 (MQ=255)
                                                                                                                                                                                                            ttATTCGATTTACT‑GCAAAATGGTGCTCAAAGAGCAGCGTTTT‑TACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGTCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGt                                      >  1:328438/1‑250 (MQ=255)
                                                                                                                                                                                                             tATTCGATT‑ACTTGCAAAATGGTGCTAAAAGAGCAGCTTTTG‑TACCCGACCTTTGACATCTCCGCTTTCCGCGGGTTCGTCAATGATTCTGACACTTGTGTTCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGGCTTTGCACACCTCGATGATCGGCCCGCACGGTGGGCCGGTTGAGGTCCAGATCCGTa                                     <  2:709215/250‑1 (MQ=255)
                                                                                                                                                                                                                 cGATTTACT‑GCAAAATGGTGCTCAAAGAGCAGCGTTTT‑TACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGa                                 >  1:188570/1‑250 (MQ=255)
                                                                                                                                                                                                                  gATTTACT‑GCAAAATGGTGCTCAAAGAGCAGCGTTTT‑TACTCGCTCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCTCGTGAAAGACTCTATCGCCATTCCAAAAACGAACGGCTATAAGTCTTTGCACACCTCGACGATAGGCCCGCACGGGGTGCCGGTTGAGGTCCCGATCCGTAcagc                                 >  2:549055/1‑246 (MQ=255)
                                                                                                                                                                                                                  actttACT‑GCAAAATGGTGCTCAAAGAGCAGCGTTTT‑TACTCGATCATGGACATCTACGCTTTCCGAGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTCCAAGACGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAACAGAGAACGGCTATCAGTCTATGCACACCCCAATGATCGGCCCGCACGGTGAGCCGGATTAGGTCCAGATCCGTACCGa                                 >  2:718905/3‑249 (MQ=255)
                                                                                                                                                                                                                    tttACT‑GCAAAATGGTGCTCAAAGAGCAGCGTTTT‑TACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGa                                 >  2:563914/1‑247 (MQ=255)
                                                                                                                                                                                                                     ttACT‑GAAAAATGGTGCTCAAAGAGCAGCGTTTG‑TACTCGATCATGGACATCGACGCTTTCCGCGTGATCGGCAATAATTCTGACACCTGTTATCGCGTGCTGGGCCAGAGGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTTCCGGTTGAGGTCCAGATCCGTACCGAAGat                             <  2:112715/250‑1 (MQ=255)
                                                                                                                                                                                                                      tACT‑GCAAAATGGTGCTCAAAGAGCAGCGTTTT‑TACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCCTTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTACAGATCCGTACCGAAGatat                           >  2:215963/1‑251 (MQ=255)
                                                                                                                                                                                                                            cAAAATGGTGCTCAAAGAGCAGCGTTTT‑TACTCTATCAGGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTACCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACgg                                                                                                      <  2:845189/171‑1 (MQ=255)
                                                                                                                                                                                                                            cAAAATGGTGCTCAAAGAGCAGCGTTTT‑TACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACgg                                                                                                      >  1:845189/1‑171 (MQ=255)
                                                                                                                                                                                                                            cAAAATGGTGCTCAAAGAGCAGCGTTTT‑TACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGACTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAACAGCGACCGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGACCCGAGCCGTACCGAAGCTATGGAcc                      >  2:129926/1‑251 (MQ=255)
                                                                                                                                                                                                                                 tGGTGCTCAAAGAGCAGCGTTTT‑TACTCGATCATGGACATCTAAGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCAAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAACGACTATATCGCCATTCCAAAAGCGATCGGCTATCAGTCTTTTCACACCTCGATGATCGGCACGCACGGTGTGCCGGTTGAGGTCCACATCCGTAccaaggtaaaggccagaatg                 >  1:564519/1‑233 (MQ=255)
                                                                                                                                                                                                                                     gatCAAAGAGCAGCGTATT‑TAATCGAACATGGCCATCTACGCTTTCCGCGTGATCGACAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACACGCCGCGTCAGGGCCGCGTGAAAGACAATATCGCACTTCCCAAAGAGAACGGCTATCAGTCTGTGCCCACCTAAATTCTCGGCACACACGGTGTGCCGGTTGCGGTACAGTACAGAACCGACGATATGGAACAGATGGCgg             >  2:748906/3‑251 (MQ=255)
                                                                                                                                                                                                                                         aaaGAGCAGCGTTTT‑TACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGCCCAGATggaggcgat         >  2:577456/1‑244 (MQ=255)
                                                                                                                                                                                                                                         aaaGAGCAGCGTTTT‑TACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGACCAGATGGCgg             <  1:577456/247‑1 (MQ=255)
                                                                                                                                                                                                                                              gcagcGTTTT‑TACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGACCAGATGGCGGGGATGGgtg    >  1:83604/1‑251 (MQ=255)
                                                                                                                                                                                                                                               cagcGTTTT‑TACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGACCAGATGGCGGAGATGGgtgt   >  1:542487/1‑251 (MQ=255)
                                                                                                                                                                                                                                               cagcGTTTT‑TACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGACCAGATGGCGGAGAGGGgtgt   >  2:495953/1‑251 (MQ=255)
                                                                                                                                                                                                                                                agcGTTTT‑TACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGACCAGATGGCGGAGATGGGTGtt  >  1:366448/1‑251 (MQ=255)
                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                  
TTAGGTATCCACCACATTAAAACCGAACTCGAAGAGCTGGGTTTTGAGGCGCTGTATCCCAACCGTTATCGCGTAATCAAAGAAGTGGTGAAAGCCGCGCGCGGCAACCGTAAA‑GAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACT‑GCAAAATGGTGCTCAAAGAGCAGCGTTTT‑CACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGACCAGATGGCGGAGATGGGTGTT  >  NC_000913/3822916‑3823404

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑