Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,707,947	C→A	intergenic (‑242/+669)	dppA ← / ← proK	dipeptide ABC transporter periplasmic binding protein/tRNA‑Pro

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,707,947	0	C	A	100.0%	205.8 / NA	64	intergenic (‑242/+669)	dppA/proK	dipeptide ABC transporter periplasmic binding protein/tRNA‑Pro
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (27/37);  total (27/37)

GCATTATTCTGCTCCAATTGTGATGTTTGTTGTTTTAACCCTTTGCAGTGGGTTGTCGCTGCCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACACACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACATTTGGTGATTAAGTTTTATGCATTTAATGAAAAAAATCTGAGGAAAAGGTGGATATCTGAGCGATTAATACCATGAACGCTAAAACGCACAGCGGAAAATGCCAGGGCCAGCCATAAGTAACGCAAAAAAAGATTTAAGCAAATATAAAAA  >  NC_000913/3707702‑3708192                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                      gCATTATTCTGCTCCAATTGTGATGTTTGTTGTTTTAACCCTTTGCAGTGGGTTGTCGCTGCCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGAAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTCCAATCTGTCAACAGATTTTGAAAACGTCAATACAGCCAACCGGGCTTTACACCCCCGGTGAGAATCCacaaacaa                                                                                                                                                                                                                                                   >  2:222646/1‑250 (MQ=255)    ttattCTGCTCCCATTGTGATGTTTGTTGTTTTATCCCTTTGCAGTGGGTTGTCGCTGCCGGACCTTTTTTGTTTCTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACCGTGCGGATCATTCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCAGGATTTACCCCAACGTTGAGAATTCACAAACAAAGa                                                                                                                                                                                                                                                <  2:73258/250‑1 (MQ=255)           tcTCCAATTGTGATGTGTGTTGTTTTAACCCTTTGCAGTGGGTTGTCGCTGCCTGACCTTTTTTTTTTTTTGCCCGGTCGGGGCAACGTTATGAGTTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTaaaaa                                                                                                                                                                                                                                         <  2:649731/249‑1 (MQ=255)           gCTCCAATTGTGATGTTTGTTGTTTTAACCCTTTGCAGTGGGTTGTCGCTGCCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACTTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTaaaaa                                                                                                                                                                                                                                         <  1:629058/250‑1 (MQ=255)              ccAATTGTGATGTTTGTTGTTTTAACCCTTTGCAGTGGGTTGTCGCTGCCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACCGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAAAATCCACAAACAATGATTAAAAAAACt                                                                                                                                                                                                                                     >  2:524207/1‑251 (MQ=255)              ccAATTGTGATGTTTGTTGTTTTAACCCTTTGCAGTGGGTTGTCGCTGCCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTaaaaaaa                                                                                                                                                                                                                                       <  1:402772/249‑1 (MQ=255)                 atcgtgATGTTTGTTGTTTTAAACCTTTGCAGTGGGTTGTCGCTGCCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTAAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCATCGTGACCTACAATCTGTCAACAGAATGAGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAAATTACAAACACTTc                                                                                                                                                                                                                                   >  1:471939/4‑250 (MQ=255)                 agtgtgATGTTTGTTGTTTTAACCCTTTGCAGTGGGGTGTCGCTGCCTGACCGTTTTTGGTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAAGGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCa                                                                                                                                                                                                                                  <  2:93899/249‑1 (MQ=255)                 aTTGTGATTTTTTTTGTTTTAACCCTTTGCAGTGGGTTGTCGCTGCCTGACCTTTTTTGTTTTTTGCCCGGTCGCGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCa                                                                                                                                                                                                                                  <  1:610506/251‑1 (MQ=255)                 aTTGTGATGTTTGTTGTTTTAACCCTTTGCAGTGGGTTGTCGCTGCCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTc                                                                                                                                                                                                                                   >  1:911389/1‑250 (MQ=255)                    gtgATGTTTGTTGTGTTAACCCTTTGCAGTGGGTGGGCGCTTCCTGACCTTTGTTGTTTTTTGCCCGTTCGGGTAAACGTTATGAGGTGGGGATGCAGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAAGCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCaaa                                                                                                                                                                                                                                <  1:437434/250‑1 (MQ=255)                         gtttggtGTTTTAACCCTTTGCAGTGGGTTGTCGCTGCCTGACCTTTTTTGTTTGTTGCCCGGTTGTGTCAACGTTATGAGGTGGGTATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACGTCAAACAGCTa                                                                                                                                                                                                                          <  2:584532/251‑1 (MQ=255)                           ttgttgTTTTAACCCTTTGAAGTGGGTTGTCGCTGCCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTAt                                                                                                                                                                                                                         <  2:604732/250‑1 (MQ=255)                                gttTTAACCCTTTGCAGTGGGTTGTCGCTGCCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAACCAAAGATTAAAAAAACTTCAACCAGCTATTTgca                                                                                                                                                                                                                    >  2:857890/1‑250 (MQ=255)                                gttTTAACCCTTTGCAGTGGGTTGTCGCTGCCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTgca                                                                                                                                                                                                                    <  2:982130/250‑1 (MQ=255)                                gttTTAACCCTTTGCAGTGGGTTGTCGCTGCCTGACCTTTTTTGTTGTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTgca                                                                                                                                                                                                                    <  1:915902/250‑1 (MQ=255)                                    tAACCCTTTGCAGTGGGTTGTCGCTGCCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGACTCCACAAACACAGATTCAAAAAACTTCAAACAGCTATTTgcagc                                                                                                                                                                                                                  >  2:826588/1‑248 (MQ=255)                                           ttGCAGTGGGTTGTCGCTGCCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAAttt                                                                                                                                                                                                        >  1:706200/1‑251 (MQ=255)                                                   ggTTGTCGCTGCCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACt                                                                                                                                                                                                >  2:12386/1‑251 (MQ=255)                                                    gTTGTCGCTGCCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTtg                                                                                                                                                                                                       >  1:560952/1‑243 (MQ=255)                                                    gTTGTCGCTGCCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTtg                                                                                                                                                                                                       <  2:560952/243‑1 (MQ=255)                                                         cGCTGCCTGACCTTTGTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAATCAGCTATTTGCAGCAGAGAAATTGGTGCTACTCCAAc                                                                                                                                                                                           <  1:669729/250‑1 (MQ=255)                                                               cTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTACAAACACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTAATCCAACATAAcc                                                                                                                                                                                     >  2:758438/1‑250 (MQ=255)                                                               cTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGAcc                                                                                                                                                                                     >  2:779992/1‑250 (MQ=255)                                                                 gACCTTTTTTGTTTTATGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGACCCTCGTGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGGGCTACTCCAACATGAcc                                                                                                                                                                                     >  2:815501/1‑248 (MQ=255)                                                                  aCCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGaa                                                                                                                                                                                 <  1:868297/251‑1 (MQ=255)                                                                   ccgtttttgttttttgCTCGTTCGGGTCAACGTGTTTAGTTGGCGATGCCGTACTAATTAACATCAGTGTAACATTGCGGATTCTCCATATAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAAATCGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGACTCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGATAAATTTGTGCTCCTCCAACATGACCAGAAc                                                                                                                                                                                <  2:212223/248‑1 (MQ=255)                                                                      tttttggttttggCCCGGTCGTGTCAACTTTATGAGGTGGGGATGCCGAACTAATTTACATCAGTGTAACTGTGCGGATCCGCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGAGTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATTTCCAGAACaa                                                                                                                                                                              <  2:737345/250‑1 (MQ=255)                                                                         ttgttttttgCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCa                                                                                                                                                                           <  2:24022/250‑1 (MQ=255)                                                                           gttttttgCCCGGTCGGGTCAACGTGATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAAAACGGCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAGACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGc                                                                                                                                                                         <  1:440374/250‑1 (MQ=255)                                                                                ttgCCCGGTCGGGTCAACGTTATTGGGTGGGGATGCCGTACTAATGAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAAGTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAat                                                                                                                                                                    <  2:952432/250‑1 (MQ=255)                                                                                 ggcacGGTAGGGTCAACGTTTGGAGGTGGGGATGCCGTACTAATTAACAGCAGTGTAACAGTGCGGATCCACCATAAAATGCACAACGTGACATACAATCTGTCAACAGAATGTGAAAACGTAAATACAGACAACCGGGATTTACACCAACGGTGAGAATTCATAAACAAAGAATAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAAGTTGTGCGACGCCAACATGATCAGAACAATCATCTTAatat                                                                                                                                                                  <  2:187288/247‑1 (MQ=255)                                                                                  gCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGAGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACAGGGATTTACACCAACGGTGAGAATACACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATAtt                                                                                                                                                                 >  1:86782/1‑251 (MQ=255)                                                                                      gggcGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAAGGCCCCGCGTGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGc                                                                                                                                                             <  2:86782/248‑1 (MQ=255)                                                                                         cGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTACAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCaa                                                                                                                                                           <  1:809877/250‑1 (MQ=255)                                                                                               aaCGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACATTTgg                                                                                                                                                    <  1:227034/251‑1 (MQ=255)                                                                                                            ggggATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACATTTGGTGATTAAGtttt                                                                                                                                        <  1:773375/250‑1 (MQ=255)                                                                                                                aTGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACATTTGGTGATTAAGTTTTATGCa                                                                                                                                   <  2:911389/251‑1 (MQ=255)                                                                                                                     gTACTAATTCACATCCGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGATTAATATTTAGCAACATTGGGTGATTAAGTTTTATGCATTTa                                                                                                                               >  2:404481/1‑250 (MQ=255)                                                                                                                     gTACTAAATAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGTTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATAGTTAGCAACATTTGGTGATTAAGTTTTATGCATTTaa                                                                                                                              <  2:567897/251‑1 (MQ=255)                                                                                                                                catcacggtAACATTGCGGATCCTACATAAAATGTCCCTCGTTACCTACAATCTGTCAACAGAAGGTGAAAACGTCAATACAGCCAATCTTGATTTACGCCAACGGTGAGAATCCACAAACAAATATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCGACTCCAATATGACCAGAACAATCAGCTTAATATTTAGCAACATTTGGTGATTAAGTTTTATGCATTTAATGAAAAAAATc                                                                                                                   <  2:873887/244‑1 (MQ=255)                                                                                                                                          aCAGGGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACATTTGGTGATTAAGTTTTATGCATTTAATGAAAAAAATCTGAGGAAAAg                                                                                                         <  2:802123/251‑1 (MQ=255)                                                                                                                                               gCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACATTTGGTGATTAAGTTTTATGCATTTAATGAAAAAAAGCTGAGGAAAAGGTgg                                                                                                     <  2:363666/250‑1 (MQ=255)                                                                                                                                                cGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATAAGCTTAATATTTAGCAACATTTGGTGATTAAGTTTTATGCATTTAATGAAACAAATCTGAGGAAAAGGTGGat                                                                                                   >  1:938241/1‑251 (MQ=255)                                                                                                                                                 ggATCCTCCATAAAATGCCCCTCGTGTCCTACAATCTGTCAACAGAATGTGAAAACGGCAATACAGCCAACCGGGATTTACACCAACGGTTAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACATTTGGTGATTAAGTTTTATGCATTTAATGAAAAAAATCTGAGGAAAAGGTGGata                                                                                                  <  1:917383/251‑1 (MQ=255)                                                                                                                                                                  ccccTCGTGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACATTTGGTGATTAAGTTTTATGCATTTAATGAAAAAAATCTGAGGAAAAGGTGGATATCTGAGCGATTAATAcc                                                                                 >  2:784138/1‑251 (MQ=255)                                                                                                                                                                  ccccTCGTGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACATTTGGTGATTAAGTTTTATGCATTTAATGAAAAAAATCTGAGGAAAAGGTGGATATATGAGCGATTAATAcc                                                                                 >  1:888673/1‑251 (MQ=255)                                                                                                                                                                   cccTCGTGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACATTTGGTGATTAAGTTTTATGCATTTAATGAAAAAAATCTGAGGAAAAGGTGGATATCTGAGCGATTAATAcc                                                                                 >  1:537372/1‑250 (MQ=255)                                                                                                                                                                        gTGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAAATTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACATTTGGTGATTAAGTTTTATGCATTTAATGAAAAAAATCTGAGGAAAAGGTGGATATCTGAGCGATTATTACAATGaa                                                                            >  2:30206/1‑250 (MQ=255)                                                                                                                                                                                      gTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACATTTGGTGATTAAGTTTTATGCATTTAATGAAAAAAATCTGAGGAAAAGGTGGATATCTGAGCGATTAATACCATGAACGCTAAAACGcac                                                               <  1:815501/249‑1 (MQ=255)                                                                                                                                                                                        caccAGACTGTGAAAACGTCAATACCGCCAAACGGGATTTACACCAACGCTGAGAATCCACCAACAAAGCTTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGAACAGAACAATCAGCTTAATATTTAGCAACATTTGGAGATTAAGTTTTATGCATTTAATGAAAAAAATCTGAGGAAAAGGGGGATATCTGAGCGATTAATACCATGAACGCAAAAACGCACAGCg                                                           >  2:980985/4‑251 (MQ=255)                                                                                                                                                                                        cAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACATTTGGTGATTAAGTTTTATGCATTTAATGAAAAAAATCTGAGGAAAAGGTGGATATCTGAGCGATTAATACCATGAACGCTAAAACGCACAGCg                                                           >  1:156340/1‑251 (MQ=255)                                                                                                                                                                                            aGAATGTGAAAACGACAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACATTTGGTGATTAAGTTTTATGCATTTAATGAAAAAAATCTGAGGAAAAGGTGGATATCTGAGCGATTAATACCATGAACGCTAAAACGCACAGCGGaaa                                                       <  2:715833/251‑1 (MQ=255)                                                                                                                                                                                               aTGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCAACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACATTTGGTGATTAAGTTTTATGCATTTAATGAAAAAAATCTGAGGAAAAGGTGGATATCTGAGCGATTAATACCATGAACGCTAAAACGCCCAGCGGAAAATg                                                    >  2:690117/1‑251 (MQ=255)                                                                                                                                                                                                  tgAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATATGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACATTTGGTGATTAAGTTTTATGCATTTAATGAAAAAAATCTGCGGTAAAGGTGGAAATCTGAGCGCTTAATACCATGAACGCTAAACCGCACAGCGGAAAATGcc                                                  >  2:981106/1‑250 (MQ=255)                                                                                                                                                                                                       atgTCAATACAGCCAACAGGAAGTTACACCAACGGTGAGAATCCACAAGCAAAGATTTAAAAAACTTCAAAAAGCTATTTGCCGCAGAGAAATTTGTGCTACGACAACATGACCAGAACAATCAGCTTAATAATTAGCAACATTTGGTGATTAAGGTGTATGCAATTAATGAAAAAAATCTGAGGAAAAGGTGGATAGCTGTGCGATTAATACCATGTACGCTAAAACGCACAGCTGAAAATGTCAGGgcc                                            <  2:701337/249‑1 (MQ=255)                                                                                                                                                                                                               aCAGCCAACCGGGATTTACACCCACGGTGAGAATCCACAAACCAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGCACAAGCAGCTTAATATATAGCAACATTTGGAGATTAAGTTTTCTGCATTTAATGAAAAAAATCTGCGGAAAAGGAGGATATCTGAGCGATTAATACCATGAACGCTAAAACGCACAGCGTAAAATGCCAAGACCAGCCATa                                     >  1:541505/1‑250 (MQ=255)                                                                                                                                                                                                                cAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACATTTGGTGATTAAGTTTTATGCATTTAATGAAAAAAATCTGAGGAAAAGGTGGATATCTGAGCGATTAATACCATGAACGCTAAAACGCACAGCGGAAAATGCCAGGGCCAGCCATa                                     >  1:250837/1‑249 (MQ=255)                                                                                                                                                                                                                    cAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACATTTGGTGATTAAGTTTTATGCATTTAATGAAAAAAATCTGAGGAAAAGGTGGATATCTGAGCGATTAATACCATGAACGCTAAAACGCACAGCGGAAAATGCCAGGGCCAGCCATAAGTAAc                               >  1:375228/1‑251 (MQ=255)                                                                                                                                                                                                                      aCCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACATTTGGTGATTAAGTTTTATGCATTTAATGAAAAAAATCTGAGGAAAAGGTGGATATCTGAGCGATTAATACCATGAACGCTAAAACGCACAGCGGAAAATGCCAGGGCCAGCCATAAGTAACGc                             <  1:222646/251‑1 (MQ=255)                                                                                                                                                                                                                          ggATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACATTTGGTGATTAAGTTTTATGCATTTAATGAAAAAAATCTGAGGAAAAGGTGGATATCTGAGCGATTAATACCATGAACGCTAAAACGCACAGCGGAAAATGCCAGGGCCAGCCATAAGTAACGCaaaa                         <  1:354196/251‑1 (MQ=255)                                                                                                                                                                                                                                           gagaATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACATTTGGTGATTAAGTTTTATGCATTTAATGAAAAAAATCTGAGGAAAAGGTGGATATCTGAGCGATTAATACCATGAACGCTAAAACGCACAGCGGAAAATGCCAGGGCCAGCCATAAGTAACGCAAAAAAAGATTTAAGCAAat         <  1:770869/250‑1 (MQ=255)                                                                                                                                                                                                                                             gaATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACATTTGGTGATTAAGTTTTATGCATTTAATGAAAAAAATCTGAGGAAAAGGTGGATATCTGAGCGATTAATACCATGAACGCTAAAACGCACAGCGGAAAATGCCAGGGCCAGCCATAAGTAACGCAAAAAAAGCTTTAAGCAAatat       >  1:547294/1‑250 (MQ=255)                                                                                                                                                                                                                                              aaTCCACAAACAAAGATTAAAAAAAATTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACATTTGGTGATTAAGTTTTATGCATTTAATGAAAAAAATCTGAGGAAAAGGTGGATATCTGAGCGATTAATACCATGAACGCTAAAACGCACAGCGGAAAATGCCAGGGCCAGCCATAAGTAACGCAAAAAAAGATTTAAGCAAAtata      >  2:417780/1‑250 (MQ=255)                                                                                                                                                                                                                                                 ccACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACATTTGGTGATTAAGTTTTATGCATTTAATGAAAAAAATCTGAGGAAAAGGTGGATATCTGAGCTATTAATACCATGAACGCTAAAACGCACAGCGGAAAATGCCAGGGCCAGCCATAAGTAACGCAAAAAAAGATTTAAGCAAATATaaaaa  <  1:587432/251‑1 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                      GCATTATTCTGCTCCAATTGTGATGTTTGTTGTTTTAACCCTTTGCAGTGGGTTGTCGCTGCCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACACACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACATTTGGTGATTAAGTTTTATGCATTTAATGAAAAAAATCTGAGGAAAAGGTGGATATCTGAGCGATTAATACCATGAACGCTAAAACGCACAGCGGAAAATGCCAGGGCCAGCCATAAGTAACGCAAAAAAAGATTTAAGCAAATATAAAAA  >  NC_000913/3707702‑3708192

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑