BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,629,921	C→T	E228K (GAG→AAG)	yhiI ←	putative membrane fusion protein YhiI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,629,921	0	C	T	98.1%	510.1 / ‑4.9	157	E228K (GAG→AAG)	yhiI	putative membrane fusion protein YhiI
Reads supporting (aligned to +/- strand): ref base C (2/1); new base T (85/68); total (87/70)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01

Only 100 of 157 total aligned reads displayed.

GGAACATCAGTTTCAGCCGTTCATCGCTGGTTTCGACGGTTTTTGGCG‑TGAACTGGGCGACACTGGCGACAAAA‑CTGATGGTTGCAG‑GAATACGCAGATCTGGCGCGGCATCGAGGATCAGCCGGGCTTCACCGCCCAGTTTCAGCGTGCCCGCCTGTTCGGTTGGCAGGAAGAAAGTCATATAGACGTCGCTGAGATCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTCGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCAATGCGCCGTTCAGTGGCTTGTGCCGCTTCGACGC‑GGGTTTGCG‑CCTGAATGATATTGGTGCGTGCCGCTTCTATAGCCGCTTTAGAAGCCGATACCTGAGCTTTCGCCGATTCCAGCGC‑AGCTCGGGCGCTCTCAGCGGCGGCGCGATCGTCATCCAGCTGTTGCGCAGAAATAGCCCC  >  NC_000913/3629675‑3630167

      cagttttCTGCCGTTCACCGCTGGTT‑CGGCGGTTTTTGGGGGTGAATGGGGCTACCGTGGCGACAAAA‑CTGTTGGTTGCAG‑GAATCCCCAGTTCTGGCGGGGCACCGAGGAGCAGCCGTGTTTCCCCGCCCAGTTTCAGCGTGCCCGCCTGTTCGGTTGGCAGGAAGAAAGTCTTATAGACGTCGCTGAGATCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGa                                                                                                                                                                                                                                                 <  2:853512/247‑1 (MQ=255)

       cAGTTTCAGCCGTTCATCGCTGGTTTCGACGGTTTTTGGCG‑TGAACTGGGCGCCACTGGCGACAAAA‑CTGATGGTTGCAG‑GAATACGCAGATCTGGCGCGGCATCGAGGATCAGCCGGGCTTCACCGACCAGTTTCAGCGTGCCCGCCTGTTCGGTTGCCAGGAAGCAAGTCATATAGCCGTCGCTGAGATCGACCATATTCAGCACCCGCCCGCCAGCCGCCAGCAATTCGCCTGGCTTGGCAAccgga                                                                                                                                                                                                                                                 >  2:775643/1‑247 (MQ=255)

          tttCAGCCGTTCATCGCTGGTTTCGACGGTTTTTGGCG‑TGAACTGGGCGACACTGGCGACAAAA‑CTGATGGTTGCAG‑GAATACGCAGATCTGGCGCGGCATCGAGGATCAGCCGGGCTTCACCGCCCAGTTTCAGCGTGCCCGCCTGTTCGGTTGGCAGGAAGAAAGTCATATAGACGTCGCTGAGATCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACt                                                                                                                                                                                                                                             >  1:85453/1‑251 (MQ=255)

             cAGCCGTTCATCGCTGGTTTCGACGGTTTTTGGCG‑TGAACTGGGCGACACTGGCGACAAAA‑CTGATGGTTGCAG‑GAATACGCAGATCTGGCGCGGCATCGAGGATCAGCCGGGCTTCACCGCCCAGTTTCAGCGTGCCCGCCTGTTCGGTTGGCAGGAAGAAAGTCATATAGACGTCGCTGAGATCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGcc                                                                                                                                                                                                                                          >  1:787293/1‑250 (MQ=255)

             cAGCCGTTCATCGCTGGTTTCGACGGTTTTTGGCG‑TGAACTGGGCGACACTGGCGACAAAA‑CTGATGGTTGCAG‑GAATACGCAGATCTGGCGCGGCATCGAGGATCAGCCGGGCTTCACCGCCCAGTTTCAGCGTGCCCGCCTGTTCGGTTGGCAGGAAGAAAGTCATATAGACGTCGCTGAGATCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGc                                                                                                                                                                                                                                           >  1:288823/1‑250 (MQ=255)

              aGCCGTTCATCGCTGGTTTCGACGGTTTTTGGCG‑TGAACTGGGCGACACTGGCGACAAAA‑CTGATGGTTGCAG‑GAATACGCAGATCTGGCGCGGCATCGAGGATCAGCCGGGCTTCACCGCCCAGTTTCAGCGTGCCCGCCTGTTCGGTTGGCAGGAAGAAAGTCATATAGACGTCGCTGAGATCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCAc                                                                                                                                                                                                                                         <  1:496386/251‑1 (MQ=255)

                  gTTCCTCGCTGGTTTCGACGGTTTTTGGCG‑TGAACTGGGCGACACTGGCGACAAAA‑CTGATGGTTGCAG‑GAATACGCAGATCTGGCGCGGCATCGAGGATCAGCCGGGCTTCACCGCCCAGTTTCAGCGTGCCCGCCTGTTCGGTTGGAAGGCATAAAGTCATATAGACGTCGCTGAGATCGACCCTATTCAGCCCCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATCCTGCAcgcgc                                                                                                                                                                                                                                     >  2:564845/1‑250 (MQ=255)

                    acATCGCTGGTTTCGAAGGTTTTTGGCG‑TGAACTGGGCGAAACTGGCGACACAA‑CTGAAGTTTGCAG‑GAATAAGCAGATCTGGCGCGGCATCGAGGATCAGCCGGGCTTCAACGCCCAGTTTCAGCGTGCCCGCCTGCTCGGTTGGCAGGAAGAAAGTCATATCGACGTCGCTGAGATCGCCCATATTCAGCACCCGACCGCCTGACGCCAGAACTTCGCCTGACTCGGCAACCCGATACTgaccgcggcc                                                                                                                                                                                                                                   >  2:850102/2‑242 (MQ=255)

                       gcGCTGGTTTCGACGGTTTTTGGCG‑TAAACGGGGCGACACGGGCGACAAAC‑CTGATGGTTGCAG‑GAATACGCAGATCGGGCGCGGCATCGGGTACCAGCCGGGCTTCCCCCCCCAGTTTAAGCGGGCCCGCCTGTTCGGTTGGCAGGAAGAAAGTCATATAGACGTCGCTGAGATCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGcgtccgtc                                                                                                                                                                                                                                <  1:533843/250‑1 (MQ=255)

                        cGCTGTTTTCGCCGGTTTTTGGCG‑TGAACGGGGCGACACTGGCGAAAAAA‑CTGATGGTTGCAG‑GAATACGCAGATCTGGCGCGGCATCGAGGATCAGCCGGGCTCCACCGCCCAGTTGCAGCGTGCCCGCCTGTTCGGTTGGCAGGAAGAAAGTCATATAGACGTCGCTGAGATCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCa                                                                                                                                                                                                                               <  1:716168/251‑1 (MQ=255)

                            ggTTTCGACGGTTTTTGGCG‑TGAACTGGGCGACTCTGGCGACAAAA‑CTGATGGTTGCAG‑GAATACGCAGATCTGGCGCGGCATCGAGGATCAGCGGGGCTTCACCGCCCAGTTTCAGCGTGCCCGCCTGTTCGGTTGGCAGGAAGAAAGTCATATAGACGTCGCTGAGATCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCAc                                                                                                                                                                                                                              <  1:617126/248‑1 (MQ=255)

                            ggTTTCGACGGTTTTTGGCG‑TGAACTGGGCGACACTGGCGACAAAA‑CTGGTGGTTGCAG‑GAATACGCAGATCTGGCGCGGCATCGAGGATCAGCCGGGCTTCACCGCCCAGTTTCAGCGTGCCCGCCTGTTCGGTTGGCAGGAAGAAAGTCATATAGACGTCGCTGAGATCGACCCTATTCAGCACCCGAACGCCTGCCGCCAGCCCTTCGCCTGGATTTGCAACCCGATACTGCACGCGTCCGTCAc                                                                                                                                                                                                                              >  2:617126/1‑248 (MQ=255)

                                cctgcGGTTTGTGGGG‑TGAACTGGGCGACACGGGCAACAAAC‑CTTAGTGTGGCAG‑GAATCCGGAGAGCGGGCGCGGCATCGAGGAGCAGCCGGGTTTCACCGCCTGTTTTCATCGTGCCCGCCGGTTCGGTTGGCAGGAAGAAAGTCATATAGACGTCGCTGAGATCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCgggg                                                                                                                                                                                                                        <  2:672256/246‑1 (MQ=255)

                                 ggACGGTTTTTGGGG‑GTAACTGGGGGACAAGGGCGAAAAAA‑CCTATGGTTTCAG‑GAATTCGCAGGCCCGGGCCGGCATCGAGGAACAGCCGGGCTTCCCCGCCCATTTTCAGGGTGCCCGCCTGTTCGGTTGGCAGGAAGAAAGTCATATAGACGTCGCTGAGATCGACCATATTCTGCACCCGTCCGCCTGCCGGCAGCGCCTCGCCTGGTTTGGCCACCCGATACTGCACGCGTCCGTCACGCGGGGCt                                                                                                                                                                                                                      <  1:155133/250‑1 (MQ=255)

                                  gACGTTTTTTGGCG‑TGAACTGGGCGACACTGGCGACAAAA‑CTGATGGTGGCAG‑GAATACGCAGATCAGGCGCGGCATCGAGGATCAGCCGGGCTTCCCCGCCCAGTTCCAGCGTGCCCGCCTGTTCGGTTGGCAGGAAGAAAGTCATATAGACGTCGCTGAGATCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCtt                                                                                                                                                                                                                     <  1:538863/251‑1 (MQ=255)

                                  gACGGTTTTTGGCG‑TGAACTGGGCGACACTGGCGACAAAA‑CTGATGGTTGCAG‑GAATACGCAGATCTGGCGCGGCATCGAGGATCAGCCGGGCTTCACCGCCCAGTTTCAGCGTGCCCGCCTGTTCGGTTGGCAGGAAGACAGTCATCTAGCCGTCGCTTAGATCGAGCATATTCAGCACCCGACCGCCTGCCGCCAGCACGTAGCCTGCCTTGGCCACCCGATGCTGCAcgcgaacggccagcggggcgt                                                                                                                                                                                                                     >  2:347062/1‑234 (MQ=255)

                                   ccGGTTTTGGGCG‑TAAACTGGGCCCCCCTGGC‑ACAAAAACTGATGGTTG‑AGTGAATACGCAGTTCGGGCGCGGCTTCGAGGATCACCCGGGCTTCACCCCCCAGTTTCACCTTCCCCGCCTGTTCGGTTGGCAGGAAGAAAGTCATATAGACGTCGCTGAGATCGACCATATTCAGCACCCGACCGCCTGCCGCCATCACTTCGCCGGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCttt                                                                                                                                                                                                                    <  1:887864/250‑1 (MQ=255)

                                        tttggggg‑TGAACGGGGCACCACGGGCGACAAAA‑CTGATGGTTGCAG‑GAATACGCAGATCTGGCGCGGCATCGAGGATCAGCCGGGCTTCACCGCCCAGTTCCAGCGTGCCCGCCTGTTCGGTTGGCAGGAAGAAAGTCATATAGACGTCGCTGAGATCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGt                                                                                                                                                                                                                <  1:72473/243‑1 (MQ=255)

                                           tGGCG‑TGAACTGGGCGACACTGGCGACAAAA‑CTGATGGTTGCAG‑GAATACGAAGATCGGGCGCGGCATCGAGGATCAGCCGGGCTTCACCGCCCAGTTTCAGCGTGCCCGCCTGTTCGGTTGGCAGGAAGAAAGTCATATAGACGTCGCTGAGATCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCg                                                                                                                                                                                                             <  1:142484/250‑1 (MQ=255)

                                                      tGGGCGACACTGGCGACAAAA‑CTGATGGTTGCAG‑GAATACGCAGATCTGGCGCGGCATCGAGGATCAGCCGGGCTTCACCGCCCAGTTTCAGCGTGCCCGCCTGTTCGGTTGGCAGGAAGAAAGTCATATAGACGTCGCTGAGATCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGat                                                                                                                                                                                                  >  1:653989/1‑251 (MQ=255)

                                                         gCGACACTGGCGACAAAA‑CTGATGGTTGCAG‑GAATAAGCAGATATGGCGCGGCATCGAGGATCAGCCGGGCTTCACCGCCCAGTTTCAGCGAGCCCGCCTGTTCGGTTAGCAGGAAGAAAGTCATATAGACGGCGCTGAGATCGACCAGATTCAGCACCCGACCGCCTGCCGACAGCACTTCGCATGGCTTGGCAACACGATTCGGCACGCGTCCGTCACGCGGGGCATACAGTTCGCTGTCATCGatgtc                                                                                                                                                                                               >  2:315659/1‑248 (MQ=255)

                                                          cGACACTGGCGACAAAA‑CTGATGGTTGCAG‑GAATACGCAGATCTGGCGCGGCATCGAGGATCAGCCGGGCTTCACCGCCCAGTTTCAGCGTGCCCGCCTGTTCGGTTGGCAGGAAGAAAGTCATATAGACGTCGCTGAGATCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCt                                                                                                                                                                                              >  1:322494/1‑251 (MQ=255)

                                                               cTGGCGACAAAA‑CTGATGGTTGCAG‑GAATACGCAGATCTGGCGCGGCATCGAGGATCAGCCGGGCTTCACCGCCCAGTTTCAGCGTGCCCGCCTGTTCGGTTGGCAGGAAGAAAGTCATATAGACGTCGCTGAGATCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGc                                                                                                                                                                                         >  1:930151/1‑251 (MQ=255)

                                                                  gCGACAAAA‑CTGATGGTTGCAG‑GAATACGCAGATCTGGCGCGGCATCGAGGATCAGCCGGGCTTCACCGCCCAGTTTCAGCGTGCCCGCCTGTTCGGTTGGCAGGAAGAAAGTCATATAGACGTCGCTGAGATCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCAAt                                                                                                                                                                                      >  1:583414/1‑251 (MQ=255)

                                                                   cGACAAAA‑CGGATGGTTGCAG‑GAATAAGCAGATCTGGCGCGGCATCGAGGATCAGCCGGGCTTCACCGCCCAGTTTCAGCGGGCCCGCCTGTTCGGTTGGCAGGACGAAAGTCATATAGACGTCGCTGAGATCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGACCGCGTCCGTCACGCGGGGCTTTCAGTTCGCAGTCATAGATATCTGCGGCAATg                                                                                                                                                                                     >  2:926740/1‑251 (MQ=255)

                                                                    gACAAAA‑CTGATGGTTGCAG‑GAATACGCAGATCTGGCGCGGCATCGAGGATCAACCGGGCTTCACCGCCCAGTTTCAGCGTGCCCGCCTGTTCGGTTGGCAGGAAGAAAGTCATATAGACGTCGCTGAGATCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCCATgc                                                                                                                                                                                    >  1:511449/1‑251 (MQ=255)

                                                                     aaaaaa‑CTGATGGTTGCAG‑GAATACGCAGATCGGGCGCGGCATCGAGGATCAGCCGGGCTTCACCGCCCAGTTTCAGCGTGCCCGCCTGTTCGGTTGGCAGGAAGAAAGTCATATAGACGTCGCTGAGATCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCAATgcg                                                                                                                                                                                   <  1:764442/249‑1 (MQ=255)

                                                                        aacactgagtgTTGCAG‑TAATCCGCAGATTGGGCGGGGCATGGAGGACCACCCGGGCTTCTCCGCCCATTTTCAGCGTGCCCGCCTGTTCGGGTGGCAGGAAGAAAGTCATATAGACGTCGCTGAGATCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCAGCTATATCTGCGGCAATGCGcc                                                                                                                                                                                 <  2:886904/241‑1 (MQ=255)

                                                                            cttaTGGTTGCAG‑GAATACGCAGATCTGGCGCGGCATCGAGGATCAGCCGGGCTTCACCGCCCAGTTTCAGCGTGCCCGCCTGTTCGGTTGGCAGGAAGAAAGTCATATAGACGTCGCTGAGATCGACCATATTCAGCACACGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGCGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCAATGCGCCGTTc                                                                                                                                                                             >  1:462989/4‑251 (MQ=255)

                                                                               aTGGTTGCAG‑GAATACGCAGATCTGGCGCGGCATCGCGGATCAGCCGGGCTTCACCGCCCAGTTTCAGCGTGCCCGCCTGTTCGGTTGGCAGGAAGAACGTCATATAGACGTCGCTGAGATCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCCCGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCAATGCGCCGTTCAGt                                                                                                                                                                          >  2:547893/1‑251 (MQ=255)

                                                                                  gTTGCAG‑GAATACGCAGATCTGGCGCGGCATCGAGGATCAGCCTGGCTTCACAGCCCAGTTTCAGCGTGCCCGCCTGTTCGGTTGGCAGGAAGAAAGTCATATAGACGTCGCTGAGATCTACCGTATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCTTCCGTCACGCGGGGCTTTCAGTTCGCTGGCATCGCTATCTGCGGCAATGCGCCGTTCAGTGGc                                                                                                                                                                       >  2:612234/1‑251 (MQ=255)

                                                                                    tGCAG‑GAATACGCAGATCTGGCGCGGCATCGAGGATCAGCCGGGCTTCACCGCCCAGTTTCAGCGTGCCCGCCTGTTCGGTTGGCAGGAAGAAAGTCATATAGACGTCGCTGAGATCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCAATGCGCCGTTCAGTGGCtt                                                                                                                                                                     >  2:388932/1‑251 (MQ=255)

                                                                                             tACGCAGATCTGGCGCGGCATCGAGGATCAGCCGGGCTTCACCGCCCAGTTTCAGCGTGCCCGCCTGTTCGGTTGGCAGGAAGAAAGTCATATAGACGTCGCTGAGATCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGCTACTGCACGCGTCCGTCACGAGGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCACTGCGCCGTTCAGTGGCTTGTGCCGCt                                                                                                                                                             >  2:210824/1‑251 (MQ=255)

                                                                                             tACGCAGATCTGGCGCGGCATCGAGGATCAGCCGGGCTTCACCGCCCAGTTTCAGCGTGCCCGCCTGTTCGGTTGGCAGGAAGAAAGTCATATAGACGTCGCTGAGATCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCAATGCGCCGTTCAGTGGCTTGTGCCGCt                                                                                                                                                             >  1:301936/1‑251 (MQ=255)

                                                                                              aCGCAGATCTGGCGCGGCATCGAGGATCAGCCGGGCTTCACCGCCCCGTTTCAGCGTGCCCGCCTGTTCGGTTGGCAGGAAGACAGTCATATAGACGTCGCTGAGATCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCAATGCGCCGTTCAGTGGCTTCTGCCGCtt                                                                                                                                                            >  2:985642/1‑251 (MQ=255)

                                                                                               cccctaccTGGGGCGGCATCGAGGATCAGCCGGGCTTCACCGCCCAGTTTCAGCGTGCCCGCCTGTTCGGTTGGCAGGAAGAAAGTCATATAGACGTCGCTGAGATCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTGCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCAATGCGCCGTTCAGTGGCTTGTGCCGCTTc                                                                                                                                                           <  1:392491/244‑1 (MQ=255)

                                                                                               cGCAGTTCGGGCGCGGCATCGGGGACCGGCCGGGCTTCCCCGCCCAGTTTCAGCGTGCCCGCCTGTTCGGTTGGCAGGAAGAAAGTCACATAGACGTCGCTGAGATCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCAATGCGCCGTTCAGTGGCTTGTGCCGCTTc                                                                                                                                                           <  1:396411/251‑1 (MQ=255)

                                                                                                  aGATCTGGCGCGGCATCGAGGATCAGCCGGGCTTCACCGCCCAGTTTCAGCGTGCCCGCCTGTTCGGTTGGCAGGAAGAAAGTCATATAGACGTCGCTGAGATCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCAATGCGCCGCTCAGGGGCtttggccgcctccgc                                                                                                                                                        >  2:835146/1‑238 (MQ=255)

                                                                                                      cTGGCGCGGCATCGAGGATCAGCCGGGCTTCACCGCCCAGTTTCAGCGTGCCCGCCTGTTCGGTTGGCAGGAAGAAAGTCATATAGACGTCGCTGAGATCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGCTATCTGCGGCACTGCGCCGTGCAGTGGCTTTGGCCGCTTCCAcggcg                                                                                                                                                    >  2:361853/1‑248 (MQ=255)

                                                                                                         gcccgGCATCGAGGATCAGCCGGGCTTCCCCGCCCATTTCCAGCGTGCCCGCCTGTTCGGTTGGCAGGAAGAAAGTCATATAGACGTCGCTGAGATCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCAATGCGACGTTCAGTGGCTTGTGCCGCTTCGACGC‑GGGtt                                                                                                                                                <  1:415648/248‑1 (MQ=255)

                                                                                                          cgcgGCATCGAGGATCAGCCGGGCTTCACCGCCCAGTTTCAGCGTGCCCGCCTGTTCGGTTGGCAGGAAGAAAGTCATATAGACGTCGCTGAGATCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCAATGCGCCGTTCAGTGGCTTGTGCCGCTTCGACGC‑GGGttt                                                                                                                                               <  1:781828/251‑1 (MQ=255)

                                                                                                            cgGCATCGAGGATCAGCCGGGCTTCACCGCCCAGTTTCAGCGTGCCCGCCTGTTCGGTTGGCAGGAAGCAAGTCATATAGACGTCGCTGAGATCGACCATATTCCGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGCTATCTGCGGCAATGCGCCGTTCCGTGGCTTGTGGCGCTTCGCCGC‑GGGTTTgt                                                                                                                                             >  2:776592/1‑250 (MQ=255)

                                                                                                            cgGCATCGAGGATCACCCGGGCTTCACCGCCCAGTTTCAGCGTGCCCGCCTGTTCGGCTGGCAGGAAGAAAGTCATATAGACGTCGCTGAGATCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCAGCGGCAATGCGCCGGTCAGTGGCTTTTACCGCCTCGACGCCGGGtttt                                                                                                                                              >  2:909560/1‑250 (MQ=255)

                                                                                                             ggcatcggggacgtgCCGGGCTTCCCCGCAAAGTTTCGGGGTGCCCGCCTGTTCGGTTGGCAGGAAGAAAGTAATATAGACGTCGCTGAGATCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCGTTCAGTTCGCTGGCATCGATATCTGCGGCAATGCGCCGTTCAGTGGCTTGTGCCGCTTCGACGC‑GGGTTTgcg                                                                                                                                            <  2:323562/237‑1 (MQ=255)

                                                                                                             ccttgcgcttttccgatcggtttCACCGCCCAGTTCCAGCGTGCCCGCCTGTTCGGTTGGCAGGAAGAAAGTCATATAGACGTCGCTGAGATCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCAATGCGCCGTACAGTGGCTTGTGCCGCTTCGACGC‑GGGTTTgcg                                                                                                                                            <  1:348299/230‑1 (MQ=255)

                                                                                                                aTCGAGGATCAGCCGGGCTTCACCGCCCAGTTTCAGCGTGCCCGCCTGTTCGGTTGGCAGGAAGAAAGTCATATAGACGTCGCTGAGATCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATAc                                                                                                                                                                                                                                              >  1:308785/1‑151 (MQ=255)

                                                                                                                     ggaccAGCCGGGCTCCACCGCCCAGTTTCAGCGTGCCCGCCTGTTCGGTTGGCAGGAAGAAAGTCATATAGACGTCGCTGAGATCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCAATGCGCCGTTCAGTGGCTTGTGCCGCTTCGACGC‑GGGTTTGCG‑CCTGAATg                                                                                                                                   <  1:45042/247‑1 (MQ=255)

                                                                                                                         cAGCCGGGCCCCACCCCCCAGTTGCGGCGTCCCCGCCTGTTCGGTTGGCAGGAAGAAAGTCATATAGACGTCGCTGAGATCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCTCTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCAATGCGCCGTTCAGTGGCTTGTGCCGCTTCGACGC‑GGGTTTGCG‑CCTGAATGatat                                                                                                                               <  1:212945/251‑1 (MQ=255)

                                                                                                                           gCCGGGCTTCACCGCCCAGTTTCAGCTTGCCCGCCTGTTCGGTTGGCAGGAAGAAAGTCATATAGACGTCGCTGAGATCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCAATGCGCCGTTCAGTGGCTTGTGCCGCTTCGACGC‑GGGTTTGCG‑CCTGAATGATATTg                                                                                                                             <  1:831943/251‑1 (MQ=255)

                                                                                                                              ggacTTCACCGCCCAGATTCAGAGTGACCGCCTGTTCGGTTGGCAGGAAGAAAGTCATATAGACGTCGCTGAGATCGACCATATTCAGCACCCGACCGCATGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCAATGCGCCGTTCAGTGGCTTGTGGCGCTTCGACGC‑AGGTTTGGG‑CCCGGATGTTATTGgtg                                                                                                                          >  2:187503/4‑251 (MQ=255)

                                                                                                                                  ttCACCGCCCAGTTTCAGCGTGCCCGCCTGTTCGGTTGGCAGGAAGAAAGTCATATAGACGTCGCTGAGATCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCAATGCGCCGTTCAGTGGCTTGTGCCGCTTCGACGC‑GGGTTTGCG‑CCTGAATGGTATTGgtgcgtg                                                                                                                      >  1:170605/1‑251 (MQ=255)

                                                                                                                                      ccGCCCAGTTTCAGCGTGCCCGCCTGTTCGGTTGGCAGGAAGAAAGTCATATAGACGTCGCTGAGATCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTCGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCAATGCGCCGTTCAGTGGCTTTTGCCGCTTCGACCC‑GGGGTTTCG‑CCCGCATGATATTGGTGCGTGACGc                                                                                                                  >  2:649883/1‑251 (MQ=255)

                                                                                                                                               ttCAGCGGGCCCGCCTGTTCGGTTGGCAGGAAGAAAGTCATATAGACGTCGCTGAGATCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCAATGCGCCGTTCAGTGGCTTGTGCCGCTTCGACGC‑GGGTTTGCG‑CCTGAATGATATTGGTGCGTGCCGCTTCTATAg                                                                                                          <  1:190703/250‑1 (MQ=255)

                                                                                                                                                tCAGCGTGCCCGCCTGTTCGGTTGGCAGGAAGAAAGTCATATAGACGTCGCTGAGATCGACCATATTCAGCACCCGACCGCCTGCCGCCCGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCAATGCGCCGTTCAGTGGCTTTTGCCGCTTCGACGC‑GGGTTTTCG‑CCTGAATGGTTTTGGTGCGTGCCggttttttagac                                                                                                        >  2:640162/1‑240 (MQ=255)

                                                                                                                                                    cGTGCCCGCCTGTTCGGTTGGCAGGAAGAAAGTCATATAGACGTCGCTGAGATCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCAATGCGCCGTTCAGTGGCTTGTGCCGCTTCGACGC‑GGGTTTGCG‑CCGGGACGATATTGgggcggggcgcctctacatgcccct                                                                                                    >  2:176868/1‑231 (MQ=255)

                                                                                                                                                        cccGCCTGTTCGGTTGGCAGGAAGAAAGTCATATAGACGTCGCTGAGATCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCAATGCGCCGTTCAGTGGCTTGTGCCGCTTAGACGC‑GGGTTTGCG‑CCTGAATGATATTGGTGCGTGCCGCTTCTATAGCCGCTTTAGa                                                                                                <  1:649578/251‑1 (MQ=255)

                                                                                                                                                         ccGCCTGTTCGGTTGGCAGGAAGAAAGTCATATAGACGTCGCTGAGATCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCAATGCGCCGTTCAGTGGCTTGTGCCGCTTCGACGC‑GGGTTTGCG‑CCTGAATGATATTGGTGCGTGCCGCTTCTATAGCCGCTTTAGaa                                                                                               <  1:605065/251‑1 (MQ=255)

                                                                                                                                                           gccggtgcGGTGGGCAGGAAGAAAGTCATATAGTCGTCGCGGAGATCGACCATATGCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCAATGCGCCGTTCAGTGGCTTGTGCCGCTTCGACGC‑GGGTTTGCG‑CCTGAATGATATTGGTGCGTGCCGCTTCTATAGCCGCTTTAGAAGc                                                                                             <  2:790807/244‑1 (MQ=255)

                                                                                                                                                            ccTGTTCGGTTGGCAGGAAGAAAGTCATATAGACGTCGCTGAGATCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCAATGCGCCGTTCAGTGGCTTGTGCCGCTTCGACGC‑GGGTTTGCG‑CCTGAATGATATTGGTGCGTGCCGCTTCTATAGCCGCTTTAGAAGcc                                                                                            <  1:511742/251‑1 (MQ=255)

                                                                                                                                                            ccTGTGCGGTTGGCAGGAAGAAAGTAATATAGACGTCTCTGAGAACGACAATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCAATGCGCCGTTCAGTGGCTTGTGCCGCTTCGACGC‑GGGTTTGCG‑CCTGAATGATATTGGTGCGTGCCGCTTCTATAGCCGCTTTAGAAGcc                                                                                            <  2:790683/251‑1 (MQ=255)

                                                                                                                                                              tGTTCGGTTGGCAGGAAGAAAGTCATATAGACGGCGCTGAGATCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCAATGCGCCGTTCAGTGGCTTGTGCCGCTTCTGCGC‑GGGTTTTCGGCCGGGATGAT‑TTGGTGGGGGCTGCTTCTATAGCCGCTTTTGAAGCCgg                                                                                          >  2:305953/1‑250 (MQ=255)

                                                                                                                                                               gTTCGGTTGGCAGGAAGAAAGTCATATAGACGTCGCTGAGATCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCAATGCGCCGTTCAGTGGCTTGTGCCGCTTCGACGC‑GGGTTTGCG‑CCTGAATGATATTGGTGCGTGCCGCTTCTATAGCCGCTTTAGAAGCCGa                                                                                          <  1:909417/250‑1 (MQ=255)

                                                                                                                                                               gTTCGGTTGGCAGGAAGAAAGTCATATAGACGTCGCTGAGATCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCAATGCGCCGTTCAGTGGCTTGTGCCGCTTCGACGC‑GGGTTTGCG‑CCTGAATGATATTGGTGCGTGCCGCTTCTATAGCCGCTTTAGAAGCCGAt                                                                                         >  1:875338/1‑251 (MQ=255)

                                                                                                                                                                 tCGGTTGGCAGGAAGAAAGTCATATAGACGTCGCTGAGATCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCAATGCGCCGTTCAGTGGCTTGTGCCGCTTCGACGC‑GGGTTTGCG‑CCTGAATGATATTGGTGCGTGCCGCTTCTATAGCCGCTTTAGAAGCCGATAc                                                                                       >  1:859404/1‑251 (MQ=255)

                                                                                                                                                                  cGGTTGGCAGGACGAAAGTCATATAGACGTCGCGGAGATCGACCAAATCCAGCACCCGGCCGCCGGCCGCCAGCGCTCCGCCTGGCGTGGCAACCCGATACTGCACGCGTCCGCCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCAATGCGCCGTTCAGTGGCTTGGGCCGCTTCGACGC‑GGGTTTGCG‑CCTGAATGAAATTGGTGCGTGCCGCTTCTATAGCCGCTTTAGAAGCCGATAc                                                                                       <  1:893331/250‑1 (MQ=255)

                                                                                                                                                                  cGGTTGGCAGGAAGAAAGTCATATAGACGTCGCTGAGATCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCAATGCGCCGTTCAGTGGCTTGTGCCGCTTCGACGC‑GGGTTTGCG‑CCTGGATGATATTGGGGCGTGCCGCTTCTATAGCCCCTTTAGAACCCGATAcc                                                                                      >  1:735621/1‑251 (MQ=255)

                                                                                                                                                                  cGGTTGGCAGGAAGAAAGTCATATAGACGTCGCTGAGATCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCAATGCGCCGTTCAGTGGCTTGTGCCGCTTCGACGC‑GGGTTTGCG‑CCTGAATGATATTGGTGCGTGCCGCTTCTATAGCCGCTTTAGAAGCCGATAcc                                                                                      <  1:198086/251‑1 (MQ=255)

                                                                                                                                                                   ggTTGGCAGGAAGAAAGTCATATAGACGTCGCTGAGATCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCAATGCGCCGTTCAGTGGCTTGTGCCGCTTCGACGC‑GGGTTTGCG‑CCTGAATGATATTGGTGCGTGCCGCTTCTATAGCCGCTTTAGAAGCCGATACCt                                                                                     <  1:821530/251‑1 (MQ=255)

                                                                                                                                                                       ggCAGGAAGAAAGTCATATAGACGTCGCTGAGATCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCCTGGCAACCCGGTACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGCTATCTGCGGCAATGCGCCGTTCAGTGGCTTGTGCCGCTTCGCCGC‑GCGCTTGCG‑GCTGTATGGTCTTGGTGCGGGCCGCTTCTCTGACCGCTTTAGAAGCCGATAcccgcgc                                                                                 >  1:460555/1‑246 (MQ=255)

                                                                                                                                                                         cagaaagaaAGTCATATAGACGGCGCTGAGATCGACCATATTCAGCCCCCGCCCGCCTGCCGCCAGCGCGTCGCCTGGCTCGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCAATGCGCCGTTCAGTGGCTTGTGCCGCTTCGACGC‑GGGTTTGCG‑CCTGAATGATAGTGGTGCGTGCCGCTTCTATAGCCGCTTTAGAAGCCGATACCTGAGCtt                                                                               <  1:850102/247‑1 (MQ=255)

                                                                                                                                                                          aGGAAGAAAGTCATATAGACGTCGCTGAGATCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCAATGCGCCGTTCAGTGGCTTGTGCCGCTTCGACGC‑GGGGTTGCG‑CCTGGATGGTATTGGTGCGTGCCGCTTCTATAGCCGCTTTAGAAGCCGATACCTGAGGttt                                                                              >  1:638057/1‑251 (MQ=255)

                                                                                                                                                                           ggAAGAAAGTCATATAGACGTCGCTGAGATCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCAATGCGCCGTTCAGTGGCTTGTGCCGCTTCGACGC‑GGGTTTGCG‑CCTGAATGATATTGGTGCGTGCCGCTTCTATAGCCGCTTTAGAAGCCGATACCTGAGCTTTc                                                                             >  1:412230/1‑251 (MQ=255)

                                                                                                                                                                                   gtcagttagcctccgctgctaTCGTCCAAATTCAGACCCCGCCCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACGGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCAATGCGCCGTTCAGTGGCTTGTGCCGCTTCGACGC‑GGGTTTGCG‑CCTGAATGATATTGGTGCGTGCCGCTTCTATAGCCGCTTTAGAAGCCGATACCTGAGCTTTCGCCGATTc                                                                     <  2:245861/231‑1 (MQ=255)

                                                                                                                                                                                   gTCATATAGACGTCGCTGAGATCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCAATGCGCCGTTCAGTGGCTTGTGCCGCTTCGACGC‑GGGTTTGCG‑CCTGAATGATATTGGTGCGTGCCGCTTCTATAGCCGCTTTAGAAGCCGATACCTGAGCTTTCGCCGATTc                                                                     <  1:688561/251‑1 (MQ=255)

                                                                                                                                                                                    tCATATAGACGTCGCTGAGATCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCAATGCGCCGTTCAGTGGCTTGGGCCGCTTCGACGC‑GGGGTTGCG‑CCCGAATGATATTTGTGCGGGCCGCTTCTTTAGCCGCTTTAGAAGCCGATACCTGAGGTTTCGcccgttcc                                                                    >  2:214226/1‑245 (MQ=255)

                                                                                                                                                                                           gACGTAGCTGAGATCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCAATGCGCCGTTCAGTGGCTTTTGCCGCTTCGAAGC‑GGGTTTGGG‑GCTGAATGGTATTGGTTCGTGACGATTCTATTGGCGCTTTTGAAGCCGATTACTGAGTTTTTGTCGATTTCAgcaccg                                                             >  2:435325/1‑247 (MQ=255)

                                                                                                                                                                                                cGCTGAGATCGACCATATTCAGCGCCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTACGGCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCAATGCGCCGTTCATTGGCTTGTGCCGCGGCGACGC‑GGGTTTGCG‑CCTGAATGATATTGGTGCGTGCCGCTTCGATAGCCGCTTTAGAAGCCGATACCTGAGCTTTCGCCGATTCCAGCGC‑AGCTCgg                                                       <  1:122438/251‑1 (MQ=255)

                                                                                                                                                                                                cGCTGAGATCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCAATGCGCCGTTCAGTGGCTTGTGCCGCTTCGACGC‑GGGTTTGCG‑CCTGAATGATATTGGTGCGTGCCGCTTCTATAGCCGCTTTAGAAGCCGGTACCTGAGCTTTCGCCGATTCCAGCGC‑AGCTCgg                                                       >  1:13259/1‑251 (MQ=255)

                                                                                                                                                                                                    gagaTCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGCTACTGCACGCGACCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCAATGCGCCGTACAGTGGCTTGTGCCGCTTCGACGC‑GGGGTTGCG‑CACGAATTATATTGGGGCCGGCCGCTTCTATTACCGATTTAGAAGCCGGTACCTGAGCTTTcccccagtcacaccccacgcaggcgc                                                    >  2:126748/1‑226 (MQ=255)

                                                                                                                                                                                                        tCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCAATGCGCCGTTCAGTGGCTTGTGCCGCTTCGACGC‑GGGTTTGCG‑CCTGAATGATATTGGTGCGTGCCGCTTCTATAGCCGCTTTAGAAGCCGATACCTGAGCTTTCGCCGa                                                                        <  1:277206/227‑1 (MQ=255)

                                                                                                                                                                                                         cGACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCAATGCGCCGTTCAGTGGCTTGTGCCGCTTCGACGC‑GGGTTTGCG‑CCTGAATGATATTGGTGCGTGCCGCTTCTATAGCCGCTTTAGAAGCCGATACCTGAGCTTTCGCCGATTCCAGCGC‑AGCTCGGGCGCTCTCa                                              <  1:369327/251‑1 (MQ=255)

                                                                                                                                                                                                            ccATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCAATGCGCCGTTCAGTGGCTTGTGCCGCTTCGACGC‑GGGGTTGCG‑CCTGAATGATATTGGTGCGTGCCGCTTCTATAGCCGCTTTTGAAGCCGCTACCTGAGCTTTCTCCGATTCCAGCGC‑AGCTCGGGCGCtctccacg                                           >  2:114105/1‑247 (MQ=255)

                                                                                                                                                                                                               tatTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCAATGCGCCGTTCAGTGGCTTGTGCCGCTTCGACGC‑GGGTTTTCG‑CCTGAATGGTATTTGTGCGTGCCGCTTATATAGCCGCTTTTGAAGCCGGTACCTGAGCTTTTCCCGTTTCCAGCCC‑AGCGCAGGCCCTCGCAgcgggg                                        >  2:864593/1‑249 (MQ=255)

                                                                                                                                                                                                                 ttCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCAATGCGCCGTTCAGTGGCTTGTGCCGCTTCGACGC‑GGGTTTGCG‑CCTGAATGATATTTGTGCGTGCCGCTTCTATAGCCGCTTTAGAAGCCGATACCTGGGCTTTCGCCGATTCCAGCGC‑AGCTCGGGGGCTCTCAgcggcggc                                      >  1:106737/1‑251 (MQ=255)

                                                                                                                                                                                                                   cAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCAATGCGCCGTTCAGTGGCTTGTGCCGCTTCGACGC‑GGGTTTGCG‑CCTGAATGATATTGGTGCGTGCCGCTTCTATAGCCGCTTTAGAAGCCGATACCTGAGCTTTCGCCGATTCCAGCGC‑AGCTCGGGCGCTCTCAGCGGCGgcgc                                    <  1:223320/251‑1 (MQ=255)

                                                                                                                                                                                                                    aGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCAATGCGCCGTTCAGTGGCTTGTGCCGCTTCGACGC‑GGGTTTGCG‑CCTGAATGATATTGGTGCGTGCCGCTTCTATAGCCGCTTTAGAAGCCGATACCTGAGCTTTCGCCGATTCCAGCGC‑AGCTCGGGCGCTCTCAGCGGCGGcgcg                                   >  1:45280/1‑251 (MQ=255)

                                                                                                                                                                                                                       cctcGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCAATGCGCCGTTCAGTGGCTTGTGCCGCTTCGACGC‑GGGTTTGCG‑CCTGAATGATATTGGTGCGTGCCGTTTCTATAGCCGCTTTAGAAGCCGATACCTGAGCTTTCGCCGATTCCAGCGC‑AGCTCGGGCGCTCTCAGCGGCGGCGCGATc                                <  1:612234/248‑1 (MQ=255)

                                                                                                                                                                                                                       ccccgcccgCCTGCCGCCAGCACTTCCCCTGGCGTGGCACCCCGAGTCTGCACGCGTCCGTCCCGGGGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCAATGCGCCGTTCAGTGGCTTGTGCCGCTTCGACGC‑GGGTTTGCG‑CCTGAATGATATTGGTGCGTGCCGCTTCTAGAGCCGCGGTAGAAGCCGATACCGGAGCGTTCGCCGATTCCAGCGC‑AGCTCGGGCGCTCGCAGCGGCGGCGCGATc                                <  2:45464/250‑1 (MQ=255)

                                                                                                                                                                                                                          cGACCGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCAATGCGCCGTTCAGTGGCTTGTGCCGCCTCGACGC‑GGGGTTGCG‑GCTGAATGGTATTGGTGCGGGCCGCTTCTCTAGACGCTTTTGAAACCGTGTCCTGTGCTTTTGCCGATTCCACCGCCAGC‑CGGGCGCTCTCCGCGGCGGGGCGacagcc                             >  2:266411/1‑246 (MQ=255)

                                                                                                                                                                                                                             ccGCCTGCCGCCAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCAATGCGCCGTTCAGTGGCTTGTGCCGCTTCGACGC‑GGGTTTGCG‑CCTGAATGATATTGGTGCGTGCCGCTTCTATAGCCGCTTTAGAAGCCGATACCTGAGCTTTCGCCGATTCCAGCGC‑AGCTCGGGCGCTCTCAGCGGCGGCGCGATCGTCATc                          <  1:157764/251‑1 (MQ=255)

                                                                                                                                                                                                                                   gccgccAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCAATGCGCCGTTCAGTGGCTTGTGCCGCTTCGACGC‑GGGTTTGCG‑CCTGAATGATATTGGTGCGTGCCGCTTCTATAGCCGCTTTAGAAGCCGATACCTGAGCTTTCGCCGATTCCAGCGC‑AGCTCGGGCGCTCTCAGCGGCGGCGCGATCGTCATCCAGc                      <  1:638322/249‑1 (MQ=255)

                                                                                                                                                                                                                                   gccgccAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCAATGCGCCGTTCAGTGGCTTGTGCCGCTTCGACGC‑GGGTTTGCG‑CCTGAATGATATTGGTGCGTGCCGCTTCTATAGCCGCTTTAGAAGCCGATACCTGAGCTTTCGCCGATTCCAGCGC‑AGCTCGGGCGCTCTCAGCGGCGGCGCGATCGTCATCCAGCTg                    <  1:672302/251‑1 (MQ=255)

                                                                                                                                                                                                                                   gccgccAGCACTTCGCCGGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCAATGCGCCGTTCAGTGGCTTGTGCCGCTTCGACGC‑GGGTTTGCG‑CCTGAATGATATTGGTGCGTGCCGCTTCTATAGCCGCTTTAGAAGCCGATACCTGAGCTTTCGCCGATTCCAGCGC‑AGCTCGGGCGCTCTCAGCGGCGGCGCGATCGTCATCCAGCTg                    <  1:301374/251‑1 (MQ=255)

                                                                                                                                                                                                                                    ccgccAGCACTTCGCCTGGCTTGGCAACCCTATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCAATGCGCCGTTCAGTGGCTTGTGCCGCTTCGACGC‑GGGTTTGCG‑CCTGAATGATATTGGTGCGTGCCGCTTCTATAGCCGCTTTAGAAGCCGATACCTGAGCTTTCGCCGATTCCAGCGC‑AGCTCGGGCGCTCTCAGCGGCGGCGCGATCGTCATCCAGCTGt                   <  1:988393/251‑1 (MQ=255)

                                                                                                                                                                                                                                      gccAGCACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCAGTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCAATGCGCCGTTCAGTGGCTTGTGCCGCCTCGACGC‑GGGTTTGCG‑CCTGAATGATATTGGTGCGTGCCGCTTCTATAGCCGCTTTAGAAGACCATACCTGGGCTTTCGCCGATTCCAGCGC‑AACTCGGGCGCTCTCAGCGGCGGAGCGGTCGTCATCCaacggttg                 >  1:329712/1‑244 (MQ=255)

                                                                                                                                                                                                                                           cACTTCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCAATGCGCCGTTCAGTGGCTTGTGCCGCTTCGACGC‑GGGTTTGCG‑CCTGAATGATATTGGTGCGTGCCGCTTCTATAGCCGCTTTAGAAGCCGATACCTGAGCTTTCGCCGATTCCAGCGC‑AGCTCGGGCGCTCTCAGCGGCGGCGCGATCGTCATCCAGCTGTTGCGCAg            <  1:781172/251‑1 (MQ=255)

                                                                                                                                                                                                                                             catCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCCCGCGGGGCTTTCAGTGCGCTGTCATCGATATCTGCGGCAATGCGCCGTTCAGTGGCTTTTGCCGCTTCGACGC‑GGGTTTGCG‑CATGAATGATATTGGTGCGTGCCGCTTCTATAGCCGCTTTAGAAGCCGATACCTGAGCTTTCGCCGATTCCAGCGC‑AGCTCGGGCGCTCTCAGCGGCGGCGCGATCGTCATCCAGCTGTTGCGCAGaa          <  1:921862/249‑1 (MQ=255)

                                                                                                                                                                                                                                               tCGCCTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCAATGCGCCGTTCAGTGGCTTGTGCCGCTTCGACGC‑GGGTTTGCG‑CCTGAATGATATTGGTGCGTGCCGCTTCTATAGCCGCTTTAGAAGCCGATACCTGAGCTTTCGCCGATTCCAGCGC‑AGCTCGGGCGCTCTCAGCGGCGGCGCGATCGTCATCCAGCTGTTGCGCAGaaa         <  1:42234/250‑1 (MQ=255)

                                                                                                                                                                                                                                                  ccTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCAATGCGCCGTTCAGTGGCTTGTTGCGCCTCGAACC‑GGGGTTCCG‑CCTGAATGCTATTGGGGCGTGACGCTTCTATAGCCCCATTGGAAGCCGCACCCTTGGCTTTCGCCCATTCCAGCGC‑CGCCCCGGCGCTCTCAGCGGCGGCGCGATCGCCATCCAGCTGtttctccgagatagc     >  2:470187/1‑238 (MQ=255)

                                                                                                                                                                                                                                                   cTGGCTTGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCAATGCGCCGTTCAGTGGCTTGTGCCGCTTCGACGC‑GGGGTTGCG‑CCTGTGTGATATTTGGGCGGGCCGCTTCTCTAGCCGCCTTAGAAGACGATACCTGGGCTTTCCCCGATTCCAGCGC‑CGCTCGAGCGCTCTCAGCCGCCTCGCGATCGGCCACCAACTGTTGCGGAGAAATAGcc    >  2:269545/1‑251 (MQ=255)

GGAACATCAGTTTCAGCCGTTCATCGCTGGTTTCGACGGTTTTTGGCG‑TGAACTGGGCGACACTGGCGACAAAA‑CTGATGGTTGCAG‑GAATACGCAGATCTGGCGCGGCATCGAGGATCAGCCGGGCTTCACCGCCCAGTTTCAGCGTGCCCGCCTGTTCGGTTGGCAGGAAGAAAGTCATATAGACGTCGCTGAGATCGACCATATTCAGCACCCGACCGCCTGCCGCCAGCACTTCGCCTGGCTCGGCAACCCGATACTGCACGCGTCCGTCACGCGGGGCTTTCAGTTCGCTGTCATCGATATCTGCGGCAATGCGCCGTTCAGTGGCTTGTGCCGCTTCGACGC‑GGGTTTGCG‑CCTGAATGATATTGGTGCGTGCCGCTTCTATAGCCGCTTTAGAAGCCGATACCTGAGCTTTCGCCGATTCCAGCGC‑AGCTCGGGCGCTCTCAGCGGCGGCGCGATCGTCATCCAGCTGTTGCGCAGAAATAGCCCC  >  NC_000913/3629675‑3630167

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`