Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,926,292	C→T	intergenic (+96/‑16)	queF → / → ppnN	7‑cyano‑7‑deazaguanine reductase/nucleotide 5'‑monophosphate nucleosidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,926,292	0	C	T	97.6%	236.7 / ‑5.2	82	intergenic (+96/‑16)	queF/ppnN	7‑cyano‑7‑deazaguanine reductase/nucleotide 5'‑monophosphate nucleosidase
Reads supporting (aligned to +/- strand):  ref base C (0/2);  new base T (42/38);  total (42/40)
Fisher's exact test for biased strand distribution p-value = 2.35e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.16e-01

GAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGCGGTCTGGACATTAACCCGTGGCGCAGTAATAGCGATTTTGTCCCATCGACCACAAGACTGGTTCGGCAATAAATTTTTTCT‑CAATTTTGCGTGCTGGATTCACGCAGAAGGTTGTGAAAGGTCATCAGGC‑AGGGCTATTGTAATCAAAGGGAATGACGATATTCGTCCCATAAGGAGTTTT‑TCTTGATTACACATATTAGCCCGCTTGGCTCCATGGA‑TATGTTG‑TC‑GCAGCTGGAAGTGGATATGCTTAAACGCACCGCCAGCAGCGACCTCTATCAACTGTTTCGCAACTGTTCACTTGCCGTACTGAACTCCGGTAGTTTGACCGATAACAGCAAAGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAAC  >  NC_000913/2926047‑2926533                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                      gAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGCGGTCTGGACATTAACCCGTGGCGCAGTAATAGCGATTTTGTCCCATCGACCACAAGACTGGTTCGGCAATAAATTTTTTCT‑CAATTTTGCGTGCTGGATTCACGCAGAAGGTTGTGAAAGGTCATCAGGC‑AGGGCTATTGTAATCAAAGGGAATGACGATATTCGTCTCATaa                                                                                                                                                                                                                                                  <  1:237059/251‑1 (MQ=255) gAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGCGGTCTGGACATTAACCCGTGGCGCAGTAATAGCGATTTTGTCCCATCGACCACAAGACTGGTTCGGCAATAAATTTTTTCT‑CAATTTTGCGTGCTGGATTCACGCAGAAGGTTGTGAAAGGTCATCAGGC‑AGGGCTATTGTAATCAAAGGGAATGACGATATTCGTCTCATa                                                                                                                                                                                                                                                   >  2:258594/1‑250 (MQ=255)   aCGCATCTTTAATGACCTGTTACGCTTATGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGGCGTGGCGGTCTGGACATTAACCCGTGGCGCAGTAATAGCGATTTTGTCCCATCGACCACAAGACTGGTTCGGCAATAAATTTTTTCT‑CAATTTTGCGTGCTGGATTCACGCAGAAGGTTGTGAAAGGTCATCAGGC‑AGGGCTATTGTAATCAAAGGGAATGACGATATTCGTCTCATAAgg                                                                                                                                                                                                                                                <  2:658333/251‑1 (MQ=255)          tttAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGCGGTCTGGACATTAACCCGTGGCGCAGTAATAGCGATTTTGTCCCATCGACCACAAGACTGGTTCGGCAATAAATTTTTTCT‑CAATTTTGCGTGCTGGATTCACGCAGAAGGTTGTGAAAGGTCATCAGGC‑AGGGCTATTGTAATCAAAGGGAATGACGATATTCGTCTCATAAGGAGtttt‑t                                                                                                                                                                                                                                        >  1:525292/1‑251 (MQ=255)           ttAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGCGGTCTGGACATTAACCCGTGGCGCAGTAATAGCGATTATGTCCCATCGACCACAAGACTGGTTCGGCAATAAATTTTTTCT‑CAATTTTGCGTGCTGGATTCACGCAGAAGGTTGTGAAAGGTCATCAGGC‑AGGGCTATTGTAATCAAAGGGAATGACGATATTCGTCTCATAAGGAGtttt‑t                                                                                                                                                                                                                                        <  1:321474/250‑1 (MQ=255)            tAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGCGGTCTGGACATTAACCCGTGGCGCAGTAATAGCGATTTTGTCCCATCGACCACAAGACTGGTTCGGCAATAAATTTTTTCT‑CAATTTTGCGTGCTGGATTCACGCAGAAGGTTGTGAAAGGTCATCAGGC‑AGGGCTATTGTAATCAAAGGGAATGACGATATTCGTCTCATAAGGAGTTTT‑TCt                                                                                                                                                                                                                                      >  1:242488/1‑251 (MQ=255)              aTGACCTGTTACGCTTCTGCCAGCCAGAAAAATGGAGCGTTTACGCACGTTATACCCGTCGTGGCGGTCTGGACATTAACCCGTGGCGCAGTAATAGCGATTTTGTCCCATCGACCACAAGACTGGTTCGGCAATAAATTTTTTCT‑CAATTTTGCGTGCTGGATTCACGCAGAAGGTTGTGAAAGGTCATCAGGC‑AGGGCTATTGTAATCAAAGGGAATGACGATATTCGTCTCATAAGGAGTTTT‑TCTTg                                                                                                                                                                                                                                    <  1:928691/251‑1 (MQ=255)                 aCCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGCGGTCTGGACATTAACCCGTGGCGCAGTAATAGCGATTTTGTCCCATCGACCACAAGACTGGTTCGGCAATAAATTTTTTCT‑CAATTTTGCGTGCTGGATTCACGCAGAAGGTTGTGAAAGGTCATCAGGC‑AGGGCTATTGTAATCAAAGGGAATGACGATATTCGTCTCATAAGGAGTTTT‑TCTTGAtt                                                                                                                                                                                                                                 >  1:190928/1‑251 (MQ=255)                    tGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGCGGTCTGGACATTAACCCGTGGCGCAGTAATAGCGATTTTGTCCCATCGACCACAAGACTGGTTCGGCAATAAATTTTTTCT‑CAATTTTGCGTGCTGGATTCACGCAGAAGGTTGTGAAAGGTCATCAGGC‑AGGGCTATTGTAATCAAAGGGAATGACGATATTCGTCTCATAAGGAGTTTT‑TCTTGATTac                                                                                                                                                                                                                               >  1:466010/1‑250 (MQ=255)                       tACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGCGGTCTGGACATTAACCCGTGGCGCAGTAATAGCGATTTTGTCCCATCGACCACAAGACTGGTTCGGCAATAAATTTTTTCT‑CAATTTTGCGTGCTGGATTCACGCAGAAGGTTGTGAAAGGTCATCAGGC‑AGGGCTATTGTAATCAAAGGGAATGACGATATTCGTCTCATAAGGAGTTTT‑TCTTGATTACACat                                                                                                                                                                                                                           >  1:839721/1‑251 (MQ=255)                        aCGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGGCGTGGCGGTCTGGACATTAACCCGTGGCGCAGTAATAGCGATTTTGTCCCATCGACCACAAGACTGGTTCGGCAATAAATTTTTTCT‑CAATTTTGCGTGCTGGATTCACGCAGAAGGTTGTGAAAGGTCATCAGGC‑AGGGCTATTGTAATCAAAGGGAATGACGATATTCGTCTCATAAGGAGTTTT‑TCTTGATTACACata                                                                                                                                                                                                                          <  1:964546/251‑1 (MQ=255)                              cTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGCGGTCTGGACATTAACCCGTGGCGCAGTAATAGCGATTTTGTCCCATCGACCACAAGACTGGTTCGGCAATAAATTTTTTCT‑CAATTTTGCGTGCTTGATTCACGCAGAAGGTTGTGAAAGGACATCAGGC‑AGGGCTATTGTAATCAAAGGGAATGACGATATTCGTCTCATAAGGAGTTTT‑TCTTGATTACACATATTAGcc                                                                                                                                                                                                                    >  1:217205/1‑251 (MQ=255)                                 ccagccagAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGCGGTCTGGACATTAACCCGTGGCGCAGTAATAGCGATTTTGTCCCATCGACCACAAGACTGGTTCGGCAATAAATTTTTTCT‑CAATTTTGCGTGCTGGATTCACGCAGAAGGTTGTGAAAGGTCATCAGGC‑AGGGCTATTGTAATCAAAGGGAATGACGATATTCGTCTCATAAGGAGTTTT‑TCTTGATTACACATATTAGCCCGc                                                                                                                                                                                                                 <  1:554906/251‑1 (MQ=255)                                 ccagccagAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGCGGTCTGGACATTAAACCGTGGCGCAGTAATCGCGATTTTGTCCCATCGACCACAAGGCTGGTTCGGCAATAAATTTTTTCT‑CAATTTTGCGTGCTGGATTAACGGCGAAGGGTGGGGAAGGTCATCAGGC‑CGGGCTCTTGTAATCACAGGGAATGACGATATTTGTGTCATAAGGAGTTTT‑TCTTGATTACACATATTAGCCCGc                                                                                                                                                                                                                 >  2:108932/1‑251 (MQ=255)                                   agccagAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGCGGTCTGGACATTAACCCGTGGCGCAGTAATATCGATTTTGTCCCATCGACCACAAGACTGGTTCGGCAATAAATTTTTTCT‑CAATTTTGCGTGCTGGATTCACGCAGAAGGTTGTGAAAGGTCATCAGGC‑AGGGCTATTGTAATCAAAGGGAATGACGATATTCGTCTCATAAGGAGTTTT‑TCTTGATTACACATATTAGCCCGCtt                                                                                                                                                                                                               <  2:728340/251‑1 (MQ=255)                                   agccagAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGCGGTCTGGACATTAACCCGTGGCGCAGTAATAGCGATTTTGTCCCATCGACCACAAGACTGGTTCGGCAATAAATTTTTTCT‑CAATTTTGCGTGCTGGATTCACGCAGAAGGTTGTGAAAGGTCATCAGGC‑AGGGCTATTGTAATCAAAGGGAATGACGATATTCGTCTCATAAGGAGTTTT‑TCTTGATTACACATATTAGCCCGCtt                                                                                                                                                                                                               >  1:416062/1‑251 (MQ=255)                                     ccagAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGCGGTCTGGACATTAACCCGTGGCGCAGTAATAGCGATTTTGTCCCATCGACCACAAGACTGGTTCGGCAATAAATTTTTTCG‑CAATTTCGCGTGCTGGATTCACGCAGAAGGTTGTGAAAGGTCATCAGGC‑AGGGCTATTGTAATCAAAGGGAATGACGATATTCGTCTCATAAGGAGTTTT‑TCTTGATTACACATATTAGCCCGCTTgg                                                                                                                                                                                                             <  2:686730/251‑1 (MQ=255)                                        gAAAAATTGAGCGTTTACGCACGTTATTCCCTTCGGGGCGGTCTGGACATTAACCCGTGGCGCAGTAATAGCGATTTTGCCCCATCGACCACAAGACTGGTTCGGCAATAAATTTTTTCT‑CAATTTTGCGTGCTGGAGTCACGCAGAAGGTTGTGAAAGGTCATCAGGC‑AGGGCTATTGTAATCAAATGGAATGACGATATTCGTCTCATAAGGAGTTTT‑TCTTGATTACACATATTAGCCCGCTTGGc                                                                                                                                                                                                            <  2:849685/249‑1 (MQ=255)                                          aaaaTTGAGCGTTTACGCACGTTATACCCGTCGTGGCGGTCTGGACATTAACCCGTGGCGCAGTAATAGCGATTTTGTCCAATCGACCACAAGACTGGTTCGGCAATAAATTTTTTCT‑CAATTTTGCGTGCTGGATTCACGCAGAAGGTTGTGAAAGGTCATCAGGC‑AGGGCTATTGTAATCAAAGGGAATGAAGATATTAGTCTCATAAGGAGTTTT‑TCTTGATTACACATATTAGCCCGCTAGGCTcc                                                                                                                                                                                                         >  1:13046/1‑250 (MQ=255)                                                 agcgtgttcGCACGGTAGACCCTTCGTGGCGGTCTGGACATTATCCCGTTTCGTAGTAATAGCGATTTTGTCCCATCGTCCACAAGACTGGTTCGGCAATAAAGTTTTTCT‑CAATTTTTCGTGCTGGATTCACGCAGAAGGTTGTGAAAGGTCATCAGGC‑AGGTCTATTGTAATCAAAGGGAATGACGATATTCGTCTCATAAGGAGTTTT‑TCTTGATTACACATATTAGCCCGCTTGGCTCCAGGGa‑tat                                                                                                                                                                                                <  2:749871/243‑1 (MQ=255)                                                      ttACGCACGTTATACCCGTCGTGGCGGTCTGGACAATACCCCGTGGCGCAGTAATAACGATTTTGTCCCCTCGACCACAAGACTGGTTCGGCAATAAATTTTTTCT‑CAATTTTGCGAGCTGGATTCACGCAGAAGGTTGTGAAAGGTCATCCGGCCAGGG‑TATTGTTATCAAAAGGGATGACGATATTCGTCTCATAAGGAGTTTT‑TTTTGATTACACATATTAGCCCGCTTGGCTCCATgggtttgtttt                                                                                                                                                                                            >  2:388649/1‑242 (MQ=255)                                                        aCGCACGTTATACCCGTCGTGGCGGTCTGGACCTTAACCCGTGGCGCAGTAATAGCGATTTTGTCCCATCGCCCACAAGACTGGTTCGGCAATAAATTTTTTCT‑CAATTTTGCGTGCTGGATTCACGCAGAAGGTTGTGAAAGGTCATCAGGC‑AGGGCTATTGTAATCAAAGGGAATGACGATATTCGTCTCATAAGGAGTTTT‑TCTTGATTACACATATTAGCCCGCTTGGCTCCCTGGG‑TAtgtttttg                                                                                                                                                                                         >  2:498600/1‑247 (MQ=255)                                                        aCGCACGTTATACCCGTCGTGGCGGTCTGGACATTAACCCGTGGCGCAGTAATAGCGATTTTGTCCCATCGACCACAAGACTGGTTCGGCAATAAATTTTTTCT‑CAATTTTGCGTGCTGGATTCACGCAGAAGGTTGTGAAAGGTCATCAGGC‑AGGGCTATTGTAATCAAAGGGAATGACGATATTCGTCTCATAAGGAGTTTT‑TCTTGATTACACATATTAGCCCGCTTGGCTCCATGGA‑TAtgttg‑t                                                                                                                                                                                          <  1:498600/249‑1 (MQ=255)                                                            aCGTTATACCCGTCGTGGCGGTCTGGACATTAACCCGTGGCGCAGTAATAGCGATTTTGTCCCATCGACCACAAGACTGGTTCGGCAATAAATTTTTTCT‑CAATTTTGCGTGCTGGATTCACGCAGAAGGTTGTGAAAGGTCATCAGGC‑AGGGCTATTGTAATCAAAGGGAATGACGATATTCGTCTCATAAGGAGTTTT‑TCTTGATTACACATATTAGCCCGCTGGGCTCCATGGA‑TATGTTG‑TC‑GCAGc                                                                                                                                                                                   <  1:307572/251‑1 (MQ=255)                                                                tattccctgcgtGGCGGTCTGGACATTAACCCTGGGCGCAGTAATAGCGATTTTGTCCCATCGACCACAAGACTGGTTCGGCAATAAATTTTTTCT‑CAATTTTGCGTGCTGGATTCACGCAGAAGGTTGTGAAAGGTCATCAGGC‑AGGGCTATTGTAATCAAAGGGAATGACGATATTCGTCTCATAAGGAGTTTT‑TCGTGATTACACATATTAGCCCGCTTGGCTCCATGGA‑TATGTTG‑TC‑GCAGCTgg                                                                                                                                                                                <  2:402883/241‑1 (MQ=255)                                                                 ataCCCGTCGTGGCGGTCTGGACATTAACCCGTGGCGCAGTAATAGCGATTTTGTCCCATCGACCACAAGACTGGTTCGGCAATAAATTTTTTCT‑CAATTTTGCGTGCTGGATTCACGCAGAAGGTTGTGAAAGGTCATCAGGC‑AGGGCTATTGTAATCAAAGGGAATGACGATATTCGTCTCATAAGGAGTTTT‑TCTTGATTACACATATTAGCCCGCTTGGCTCCATGGA‑TATGTTG‑TC‑GCAGCTGGa                                                                                                                                                                               >  1:630021/1‑250 (MQ=255)                                                                     ccGTCGTGGCGGTCTGGACATTAACCCGTGGCGCAGTAATAGCGATTTTGTCCCATCGACCACAAGACTGGTTCGGCAATAAATTTTTTCT‑CAATTTTGCGTGCTGGATTCACGCAGAAGGTTGTGAAAGGTCATCAGGC‑AGGGCTATTGTAATCAAAGGGAATGACGATATTCGTCTCATAAGGAGTTTT‑TCTTGATTACACATATTAGCCCGCTTGGCTCCATGGA‑TATGTTG‑TC‑GCAGCTGGAAGTgg                                                                                                                                                                          <  2:698460/251‑1 (MQ=255)                                                                       gtcgtGGCGGGCTGGACATTAACCCGTGGCGCAGTAATAGCGATTTTGTCCCATCGACCACAAGACTGGTTCGGCAATAAATTTTTTCT‑CAATTTTGCGTGCTGGATTCACGCAGAAGGTTGTGAAAGGTCATCAGGC‑AGGGCTATTGTAATCAAAGGGAATGACGATATTCGTCTCATAAGGAGTTTT‑TCTTGATTACACATATTAGCCCGCTTGGCTCCATGGA‑TATGTTG‑TC‑GCAGCTGGAAGTGGat                                                                                                                                                                        <  2:230135/251‑1 (MQ=255)                                                                       ggcgtGGCGGTCTGGACATTAACCCGTGGCGCAGTAATAGCGATTTTGTCCCATCGACCACAAGACTGGTTCGGCAATAAATTTTTTCT‑CAATTTTGCGTGCTGGATTCACGCAGAAGGTTGTGAAAGGTCATCAGGC‑AGGGCTATTGTAATCAAAGGGAATGACGATATTCGTCTCATAAGGAGTTTT‑TCTTGATTACACATATTAGCCCGCTTGGCTCCATGGA‑TATGTTG‑TC‑GCAGCTGGAAGTGGat                                                                                                                                                                        <  1:613989/249‑1 (MQ=255)                                                                              cGGTCTGGACATTAACCCGTGGCGCAGTAATAGCGATTTTGTCCCATCGACCACAAGACTGGTTCGGCAATAAATTTTTTCT‑CAATTTTGCGTGCTGGATTCACGCAGAAGGTTGTGAAAGGTCATCAGGC‑AGGGCTATTGTAATCAAAGGGAATGACGATATTCGTCTCATAAGGAGTTTT‑TCTTGATTACACATATTAGCCCGCTTGGCTCCATGGA‑TATGTTG‑TC‑GCAGCTGGAAGTGGATATGCTTa                                                                                                                                                                 <  1:240972/251‑1 (MQ=255)                                                                               ggTCTGGTCATTAACCCTTGGAGCAGTAATAGCGATTTTGGCCCATCGACCACAAGACTGGTTCGGCAAGAAATTTTTTCT‑CAATTTTGCGTGCTGGATTCACGCAGAAGGTTGTGAAAGGTCATCAGGC‑AGGGCTATTGTAATCAAAGGGAATGACGATATTCGTCTCATAAGGAGTTTG‑TCTTGATTACACATATTAGCCCGCTTGGCTCCATGGA‑TATGTTG‑TC‑GCAGCTGTAAGTGGATATGCTTaa                                                                                                                                                                <  2:463421/251‑1 (MQ=255)                                                                                    ggACATTAACCCGTGGCGCAGTAATAGCGATTTTGTCCCATCGACCACAAGACTGGTTCGGCAATAAATTTTTTCT‑CAATTTTGCGTGCTGGATTCACGCAGAAGGTTGTGAAAGGTCATCAGGC‑AGGGCTATTGTAATCAAAGGGAATGACGATATTCGTCTCATAAGGAGTTTT‑TCTTGATTACACATATTAGCCCGCTTGGCTCCATGGA‑TATGTTG‑TC‑GCAGCTGGAAGTGGATATGCTTAAACGc                                                                                                                                                            >  1:127987/1‑250 (MQ=255)                                                                                                 tGGCGCAGTAATAGCGATTTTGTCCCATCGACCACAAGACTGGTTCGGAAATAAATTTTTTCT‑CAATTTTGCGTGCTGGATTCACGCAGAAGGTTGTGAAAGGTCATCAGGC‑AGGGCTATTGTAATCAAAGGGAATGACGATATTCGTCTCATAAGGAGTTTT‑TCTTGATTACACATATTAGCCCGCTTGGCTCCATGGA‑TATGTTG‑TC‑GCAGCTGGAAGTGGATATGCTTAAACGCACCGCCAGCAGCg                                                                                                                                               >  2:672225/1‑250 (MQ=255)                                                                                                    cgctctaaaagccaTTTTGTCCCAGAGACCACAAGACTGGTTCTGCAATAAATTTTTTCT‑AAATTTGGCGTGCTGGATTCACGCAGAAGGTTGTGAAAGGTCATCAGGC‑GGGGCTATTTTAATCAAAGGGAATGACGATATTCGTCTCATAAGGAGTTGT‑TCGTGATTACACATATTAGCCCGCTTGGCTCCATGGA‑CATTTGG‑TC‑GAAGCTTGAAGTGGATATGCTTAAACGCACCGCCAGCAGCGTCct                                                                                                                                           <  2:182437/238‑1 (MQ=255)                                                                                                     gcAGTAATAGCGATTTTGTCCCATCGACCACAAGACTGGTTCGGCAATAAATTTTTTCT‑CAATTTTGCGTGCTGGATTCACGCAGAAGGTTGTGAAAGGTCATCAGGC‑AGGGCTATTGTAATCAAAGGGAATGACGATATTCGTCTCATAAGGAGTTTT‑TCTTGATTACACATATTAGCCCGCTTGGCTCCATGGA‑TATGTTG‑TC‑GCAGCTGGAAGTGGATATGCTTAACAGCACCGCCAGCAGCGACctc                                                                                                                                          >  1:543243/1‑251 (MQ=255)                                                                                                               cgtttttGTCCCATGGCCCACAAGATTGGTTCGGCAATAAATTTTTTCT‑CAATTGTGCGTTCTGGATTCACGCAGAAGTGTGTTAAAGTTCTTCAGGC‑AGGGCTATTGTAATCAAAGGGAATGACGATATTCGTCTCATAAGGAGTTTT‑TTTTGAGTACTCATATTAGCCTGCTTGGCTCCATTGA‑TATGTTG‑TC‑GCAGCTGGAAGTGTATATGCTTAAACGCACCGCCAGCAGCGACCTCTATCAACTGt                                                                                                                                <  2:594683/248‑1 (MQ=255)                                                                                                                  ttttGTCCCATCGACCACAAGACTGGTTCGGCAATAAATTTTTTCT‑CAATTTTGCGTGCTGGATTCACGCAGAAGGTTGTGAAAGGTCATCAGGC‑AGGGCTATTGTAATCAAAGGGAATGACGATATTCGTCTCATAAGGAGTTTT‑TCTTGATTACACATATTAGCCCGCTTGGCTCCATGGA‑TATGTTG‑TC‑GCAGCTGGAAGTGGATATGCTTAAACGCACCGCCAGCAGCGACCTCTATCAACTGTTTc                                                                                                                             >  1:177483/1‑251 (MQ=255)                                                                                                                       tCCCATCGACCACAAGACTGGTTCGGCAATAAATTTTTTCT‑CAATTTTGCGTGCTGGATTCACGCAGAAGGTTGTGACAGGTCATCAGGC‑AGGGCTATTGTAATCAAAGGGAATGACGATATTCGTCTCATAAGGAGTTTT‑TCTTGATTACACATATTAGCCCGCTTGGCTCCATGGA‑TATGTTG‑TC‑GCAGCTGGAAGTGGATATGCTTAAACGCACCGCCAGCAGCGACCTCTATCAACTGTTTCGCAAc                                                                                                                        >  1:934112/1‑251 (MQ=255)                                                                                                                         ccATCGACCACAAGACTGGTTCGGCAATAAATTTTTTCT‑CAATTTTTCGTGCTGGATTCACGCAGACGGTTGTGAAAGGTCATCAGGC‑AGGGCTATTGTAATCAAAGGGAATGACGATATTCTTCTCATAAGGAGTTTT‑TCTTGATTACACATATTAGCCCGCTTGGCTCCATGGA‑TATGTTG‑TC‑GCTGCTGGAAGTGGATATGCTTAAACGCCCCGCCAGCAGCGCCCTCTATCAACTGTTTCGCAACTg                                                                                                                      >  2:235626/1‑251 (MQ=255)                                                                                                                          cATCGACCACAAGACTGGTTCGGCAATAAATTTTTTCT‑CAATTTTGCGTGCTGGATTCACGCAGAAGGTTGTGAAAGGTCATCAGGC‑AGGGCTATTGTAATCAAAGGGAATGACGATATTCGTCTCATAAGGAGTTTT‑TCTTGATTACACATATTAGCCCGCTTGGCTCCATGGA‑TATGTTG‑TC‑GCAGCTGGAAGTGGATATGCTTAAACGCACCGCCAGCAGCGACCTCTATCAACTGTTTCGCAACTGt                                                                                                                     >  1:837656/1‑251 (MQ=255)                                                                                                                           aacgcccACAATATTGGTTCGGCAATAAATTTTT‑CTGCAATTTGGCGTGCTGGTTTCCCGCAGAAGTTTGTAAAAGGTTATCAGGC‑AGGGTTATTTTAATCAAAGGGAATGACGATTTTCTTTCCTTAAGGAGTTTT‑TTTTGATTACACATATTATCCCACTTGGCTCC‑TGGAATATTTTG‑TC‑TCAGTTGGAAGTGGATATGCTTAAACGAACCGCTAGCAGCGACCTCTATCAACTGTTTCGCAACTGtt                                                                                                                    <  2:383195/246‑1 (MQ=255)                                                                                                                           aTCGACCACAAGACTGGTTCGGCAATAAATTTTTTCT‑CAATTTTGCGTGCTGGATTCACGCAGAAGGTTGTGAAAGGTCATCAGGC‑AGGGCTATTGTAATCAAAGGGAATGACGATATTCGTCTCATAAGGAGTTTT‑TCTTGATTACACATATTAGCCCGCTTGGCTCCATGGA‑TATGTTG‑TC‑GCAGCTGGAAGTGGATATGCTTAAACGCACCGCCAGCAGCGACCTCTATCAACTGTTTCGCAACTGtt                                                                                                                    >  1:494020/1‑251 (MQ=255)                                                                                                                                          ggTTCGGCAATAAATTTTTTCT‑CAATTTTGCGTGCTGGATTCACGCAGAAGGTTGTGAAAGGTCATCAGGC‑AGGGCTATTGTAATCAAAGGGAATGACGATATTCGTCTCATAAGGAGTTTT‑TCTTGATTACACATATTAGCCCGCTTGGCTCCATGGA‑TATGTTG‑TC‑GCAGCTGGAAGTGGATATGCTTAAACGCACCGCCAGCAGCGACCTCTATCAACTGTTTCGCAACTGTTCACTTGCCGTACTg                                                                                                      >  1:575489/1‑250 (MQ=255)                                                                                                                                           gTTCGGCAATAAATTTTTTCT‑CAATTTTGCGTGCTGGATTCACGCAGAAGGTTGTGAAAGGTCATCAGGA‑AGGGCTATTGTAATCAAAGGGAATGACGATATTCGTCTCATAAGGAGTTTT‑TCTTGATTACACATATTAGCCCGCTTGGCTCCATGGA‑TATGTTG‑TC‑GCAGCTGGAAGTGGATATGCTTAAACGCACCGCCAGCAGCGACCTCTATCAACTGTTTCGCAACTGTTCACTTGCCGTACTGa                                                                                                     >  1:115211/1‑250 (MQ=255)                                                                                                                                                     aaaTTTTTTCT‑CAATTTTGCGTGCTGTATTCACGCAGAAGGTTGTGAAAGTTCATCAGGC‑AGGGCTATTGTAATCAAAGGGAATGACGATATTCGTCTCATAAGGATTTTT‑TCTTGATTACACATATTAGCCCGTTTGGCTCCATGGA‑TATGTTG‑TC‑GCAGCTGGAAGTGGATATGCTTAAACGCACCGCCAGCAGCGACCTCTATCAACTGTTTCGCAACTGTTCTCTTGCCGTACTGAACTCCGGTAGt                                                                                          <  1:860643/251‑1 (MQ=255)                                                                                                                                                      aaTTTTTTCT‑CAATTGTGCGTGCTGGATTCACGCAGAAGGTTGTGAATGGTCTTCAGGC‑AGGGCTATTGTAATCAAAGGGAATGACGATATGCGTCTCATAAGGAGTTTT‑TCTTGATTACACATCTTATCCCTCTTGGCTCCATGGA‑TATGTGG‑TC‑GCAGCTGGAAGAGGATATGCTTAAACGCCCCGCCAGCAGCGACCTCTATCAACTGTTTCGCAACTGTTCACTTGCCGTACTGAACTCCGGTAGtt                                                                                         <  2:702622/251‑1 (MQ=255)                                                                                                                                                       aTTTTTTCT‑CAATTTTGCGTGCTGGATTCACGCCGAAGGTTGTGAAAGGTCATCAGGC‑AGGGCTATTGTAATCAAAGGGAATGACGATATTCGTCTCATAAGGAGTTTT‑TCTTGATTACACATATTAGCCCGCTTGGCTCCATGGA‑TATGTTG‑TC‑GCAGCTGGAAGTGGATATGCTTAAACGCACCGCCAGCAGCGACCTCTATCAACTGTTTCGCAACTGTTCACTTGCCGTACTGAACTCCGGTAGttt                                                                                        >  2:579346/1‑251 (MQ=255)                                                                                                                                                       aTTTTTTCT‑CAATTTTGCGTGCTGGATTCACGCAGAAGGTTGTGAAAGGTCATCAGGC‑AGGGCTATTGTAATCAAAGGGAATGACGATATTCGTCTCATAAGGAGTTTT‑TCTTGATTACACATATTAGCCCGCTTGGCTCCATGGA‑TATGTTG‑TC‑GCAGCTGGAAGTGGATATGCTTAAACGCACCGCCAGCAGCGACCTCTATCAACTGTTTCGCAACTGTTCACTTGCCGTACTGAACTCCGGTAGttt                                                                                        >  1:487392/1‑251 (MQ=255)                                                                                                                                                          ttttCT‑CAATTTTGCGTGCTGGATTCACGCAGAAGGTTGTGAAAGGTCATCAGGC‑AGGGCTATTGTAATCAAAGGGAATGACGATATTCGTCTCATAAGGAGTTTT‑TCTTGATTACACATATTAGCCCGCTTGGCTCCATGGA‑TATGTTG‑TC‑GCAGCTGGAAGTGGATATGCTTAAACGCACCGCCAGCAGCGACCTCTATCAACTGTTTCGCAACTGTTCACTTGCCGTCCTGACCTCCGGTAGTTTGAc                                                                                     >  2:367031/1‑251 (MQ=255)                                                                                                                                                            ttCT‑CAATTTTGCGTGCTGGATTCACGCAGAAGGTTGTGAAAGGTCATCAGGC‑AGGGCTATTGTAATCAAAGGGAATGACGATATTCGTCTCATAAGGAGTTTT‑TCTTGATTACACATATTAGCCCGCTTGGCTCCATGGA‑TATGTTG‑TC‑GCAGCTGGAAGTGGATATGCTTAAACGCACCGCCAGCAGCGACCTCTATCAACTGTTTCGCAACTGTTCACTTGCCGTACTGAACTCCGGTAGTTTGAcc                                                                                    <  1:258594/250‑1 (MQ=255)                                                                                                                                                                     tttGGGTGCTGGATTCACGCAGAAGGTTTTGAAAGGTCATCAGGA‑AGGGCTATTGTAACCAAAGGGAATGACGTTATTCGTCTTATAAGGAGTTTT‑TCTTGATTACACATATTAGCCCGCTTGGCTCCATGGA‑TATGTTG‑TC‑GCAGCTGGAAGTGGATATGCTTAAACGCACCGCCAGCAGCGACCTCTATCAACTGTTTCGCAACTGTTCACTTGCCGTACTGAACTCCGGTAGTTTGACCGATAACAg                                                                            <  2:525292/250‑1 (MQ=255)                                                                                                                                                                         cGTGCTGGATTCACGCAGAAGGTTGTGAAAGGTCATCAGGC‑AGGGCTATTGTAATCAAAGGGAATGACGATATTCGTCTCATAAGGAGTTTT‑TCTTGATTACACATATTAGCCCGCTTGGCTCCATGGA‑TATGTTG‑TC‑GCAGCTGGAAGGGGAGATGCTTAAACGCACCGCCAGCAGCGACCTCTATCAACTGTTTCGCAACTGTTCACTTGCCGTACTGAACTCCGGTAGTTTGACCGATAACAGCAAAg                                                                       <  1:50277/251‑1 (MQ=255)                                                                                                                                                                           tGCTGGATTCACGCAGAAGGTTGTGAAAGGTCATCAGGC‑AGGGCTATTGTAATCAAAGGGAATGACGATATTCGTCTCATAAGGAGTTTT‑TCTTGATTACACATATTAGCCCGCTTGGCTCCATGGA‑TATGTTG‑TC‑GCAGCTGGAAGTGGATATGCTTAAACGCACCGCCAGCAGCGACCTCTATCAACTGTTTCGCAACTGTTCACTTGCCGTACTGAACTCCGGTAGTTTGACCGATAACAGCAAAGa                                                                      >  2:797823/1‑250 (MQ=255)                                                                                                                                                                           tGCTGGATTCACGCAGAAGGTTGTGAAAGGTCATCAGGC‑AGGGCTATTGTAATCAAAGGGAATGACGATATTCGTCTCATAAGGAGTTTT‑TCTTGATTACACATATTAGCCCGCTTGGCTCCATGGA‑TATGTTG‑TC‑GCAGCTGGAAGTGGATATGCTTAAACGCACCGCCAGCAGCGACCTCTATCAACTGTTTCGCAACTGTTCACTTGCCGGACTGAACTCCGGTAGTTTGACCGATAACAGCAAAGa                                                                      >  2:591754/1‑250 (MQ=255)                                                                                                                                                                             cTGGATTCACGCAGAAGGTTGTGAAAGGTCATCAGGC‑AGGGCTATTGTAATCAAAGGGAATGACGATATTCGTCTCATAAGGAGTTTT‑TCTTGATTACACATATTAGCCCGCTTGGCTCCATGGA‑TATGTTG‑TC‑GCAGCTGGAAGTGGATATGCTTAAACGCACCGCCAGCAGCGACCTCTATCAACTGTTTCGCAACTGTTCACTTGCCGTACTGAACTCCGGTAGTTTGACCGATAACAGCAAAGAAtt                                                                   >  2:45335/1‑251 (MQ=255)                                                                                                                                                                                    cACGCAGACGGTTGTGACAGGTCATCAGGC‑AGGGCTATTGTCATCCAAGGGAATGACGATATTCGTATCATAAGGAGTTTT‑TCTTGATTACACATATTAGCCCGCTTGGCTCCATGGA‑TATGTTG‑TC‑GCAGCTGGAAGTGGCTATGCTTTAACGCACCGCCAGCAGCGACCTCTATCACATGTTTCGCAACTGTTCACTTGCCGTAATGACCTCCGGTAGTTTGACCGATAACAGCAAAGCATTGCTGtct                                                            >  2:950158/1‑251 (MQ=255)                                                                                                                                                                                     aCGCAGAAGGTTGTGAAAGGTCATCAGGC‑AGGGCTATTGTAATCAAAGGGAATGACGATAATCGTATCATAAGGAGTTTT‑TCTTGATTACACATATTAGCACGCTTGGCTCCATGGA‑TATGTTG‑TC‑GCAGCTGGAAGTGGATATGCATAAACGCACCGCCAGCAGCGACCTATATCAAAGGTTTCGCAACTGTTCACTAGACGTACTGAACTCCGGTAGTTTGACCGATAAAAGCAAAGACTTGCTGtctc                                                           >  2:783970/1‑251 (MQ=255)                                                                                                                                                                                              gTTGTGAAAGGTCATCAGGC‑AGGGCTATTGTAATCAAAGGGAATGACGATATTCGTCTCATAAGGAGTTTT‑TCTTGATTACACATATTAGCCCGCTTGGCTCCATGGA‑TCTGTTG‑TC‑GCAGCTGGAAGTGGATATGCTTAAACGCACCGCCAGCAGCGACCTCTATCAACTGTTTCGCAACTGTTCACTTGCCGTACTGAACTCCGGTAGTTTGACCGATAACAGCAAAGAATTGCTGTCTCGTTTTGaa                                                   >  1:952793/1‑250 (MQ=255)                                                                                                                                                                                                 gtgAAAGGTCATCAGGC‑AGGGCTATTGTAATCAAAGGGAATGACGATATTCGTCTCATAAGGAGTTTT‑TCTTGATTACACATATTAGCCCGCTTGGCTCCATGGA‑TATGTTG‑TC‑GCAGCTGGAAGTGGATATGCTTAAACGCACCGCCAGCAGCGACCTCTATCAACTGTTTCGCAACTGTTCACTTGCCGTACTGAACTCCGGTAGTTTGACCGATAACAGCAAAGAATTGCTGTCTCGTTTTGAAAAtt                                               >  1:171948/1‑251 (MQ=255)                                                                                                                                                                                                    aaaGTTCATCATGC‑AGGTCTATTGTAATCAAAGGGAATGACGATTTTCGTCTAATAAGGAGTTTT‑TCTTGATTACACATATTAGCCCGCTTGGCCCCATGGA‑TATGTGG‑TC‑GCAGCTGGAAGTGGATATGCTTAAACGCACCGCCAGCAGCGACCTCTATCAACTGTTTCGCAACTGTTCACTTGCCGTACTGAACTCCGGTAGTTTGACCGATAACAGCAAAGAATTGCTGTCTCGTTTTGAAAATTTCg                                            <  2:494020/251‑1 (MQ=255)                                                                                                                                                                                                      agatcatcaGGC‑AGTGCAATTGTAGTCAAAGGTAAGGAGGAGATTCGCCTCATAAGGATTTTT‑TCTTGATTACGCATATGAGCCCGCTGGACCACACGGA‑TA‑CTTG‑TCCGCAGCTGGAAGTGGATAGGCAAAAAGGCACCGCCAGCAGCGACCTCTATCAACTGTTTCGCAACTGTTCACTTGCCGTACTGATCTACGGTAGTTTGACCGATAACAGCAAAGAATTGCTGTCTCGTTTTGAAAATTTCGat                                          <  2:258450/248‑1 (MQ=255)                                                                                                                                                                                                       ggTCATCAGGC‑AGGGCTATTGTAATCAAAGGGAATGACGATATTCGTCTCATAAGGAGTTTT‑TCTTGATTACACATATTAGCCCGCTTGGCTCCATGGA‑TATGTTG‑TC‑GCAGCTGGAAGTGGATATGCTTAAACGCACCGCCAGCAGCGACCTCTATCAACTGTTTCGCAACTGTTCACTTGCCGTACTGACCTCCGGTAGTTTGACCGATAACAGCAAAGAATTGCTGTCTCGTTTTGAAAATTTCGat                                          >  2:71965/1‑250 (MQ=255)                                                                                                                                                                                                       ggTCATCAAGC‑GGGGGTATTGAAATAAAAGATAATGACGATTTGCGTCTCTCACGGAGTTTT‑TATTTATTACAAATATTAGCCCTCTTGGATCCATGGA‑TATGTTG‑TC‑TCAGCGGGAATTGGATATGCTAAAACGCACCGCCAGCAGCGACCTCTATCAGCTGTTACGTAACTGTTCACTTGACGTACTGAACTCCGGTAGTTTGACCGATAACAGCAAAGAATTGCTGTCTCTTTTTGAAAATTTCGata                                         <  2:234020/251‑1 (MQ=255)                                                                                                                                                                                                        gTCATCAGGC‑AGGGCTATTGTAATCAAAGGGAATGACGATATTCGTCTCATAAGGAGTTTT‑TCTTGATTACACATATTAGCCCGCTTGGCTCCATGGA‑TATGTTG‑TC‑GCAGCTGGAAGTGGATATGCTTAAACGCACCGCCAGCAGCGACCTCTATCAACTGTTTCGCAACTGTTCACTTGCCGTACTGAACTCCGGTAGTTTGACCGATAACAGCAAAGAATTGCTGTCTCGTTTTGAAAATTTCGatat                                        >  1:735284/1‑251 (MQ=255)                                                                                                                                                                                                          catcaGGC‑AGGGCTATTGTAATCAAAGGGAATGACGATATTCGTCTCATAAGGAGTTTT‑TCTTGATTACACATATTCGCCCGCTTGGCTCCATGGA‑TATGTTG‑TC‑GCAGCTGGACGTGGATATGCTTAAACGCACCGCCAGCAGCGACCTCTATCAACTGTTTCGCAACTGTTCACTTGCCGTACTGAACTCCGGTAGTTTGACCGATAACAGCAAAGAATTGCTGTCTCGTTTTGAAAATTTCGTTAtt                                       >  1:970824/1‑250 (MQ=255)                                                                                                                                                                                                               ggc‑aggTCTATTGTAATCAAAGGGAATGACGATATTCGTCCCATAAGGAGTTTT‑TCTTGATTACCCATTTTAGCCCGCTTGGCTCCATGGA‑TATGTTG‑GC‑GCAGCTGGAAGGGGATATGCTTAAACGCACCGCCAGCAGCGACCTCTATCAACTGTTTCGCAACTGTTCACTTGCCGTACTGAACTCCGGTAGTTTGACCGATAACAGCAAAGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTc                                 <  1:507696/251‑1 (MQ=255)                                                                                                                                                                                                               ggc‑aggGCTATTGTAATCAAAGGGAATGACGATATTGGTCTCATAAGGAGTTTT‑TCTTGATTACACATATTAGCCCGCTTGGCTCCATGGA‑TATGTTG‑TC‑GCAGCTGGAAGTGGATATGCTTAAACGCACCGCCAGCAGCGACCTCTATCAACTGTTTCGCAACTGTTCACTTGCCGTACTGAACTCCGGTAGTTTGACCGATACCAGCAAAAAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTc                                 >  2:929045/1‑251 (MQ=255)                                                                                                                                                                                                                   aggGCTATTGTAATCAAAGGGAATGACGATATTCGTCTCATAAGGAGTTTT‑TCTTGATTACACATATTAGCCCGCTTGGCTCCATGGA‑TATGTTG‑TC‑GCAGCTGGAAGTGGATATGCTTAAACGCACCGCCAGCAGCGACCTCTATCAACTGTTTCGCAACTGTTCACTTGCCGTACTGCACTCCGGTAGTTTGACCGATAACAGCAAAGTATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTg                              >  2:638457/1‑251 (MQ=255)                                                                                                                                                                                                                    gggCTACTGTAATCAAAGGGCATGACGATATTCGTCTCATAAGGAGCTTT‑TCTTGATTACACATATTAGCCCGCTTGGCTCCATGGA‑TATGTTG‑TC‑GCAGCTGGAAGTGGATATGCTTAAACGCACCGCCAGCAGCGACCTATATCAACTGTTTCGCAACTGTTCACTTGCCGTACTGAACTCCGGTAGTTTGACCGATACCAGCAAAGAATTGTTGTCTCGTTTTGAACATTTCGCTATTaaagtactgc                             >  2:465906/1‑243 (MQ=255)                                                                                                                                                                                                                        tATTGTAATCAAAGGGAATGACGATATTCGTCTCATAAGGAGTTTT‑TCTTGATTACACATATTAGCCCGCTTGGCTCCATGGA‑TATGTTG‑TC‑GCAGCTGGAAGTGGATATGCTTAAACGCACCGCCAGCAGCGACCTCTATCAACTGTTTCGCAACTGTTCACTTGACGTACTGAACTCCGGTAGTTTGACCGATAACAGCAAAGAATTGCTGGCCCGTTTTGAAAATTTCGATATTAACGTCTTGCTCCg                         >  2:628986/1‑251 (MQ=255)                                                                                                                                                                                                                           tGTAATCAAAGGGAATGACTATATTCGTCTCATAAGGAGTTTT‑TCGTGATCACACATATTAGCCCGCTTGGCTCCATGGA‑TATGTTG‑TC‑GCAGCTGGAAGGGGATATGGTTAAGCGCTCCGCCAGCAGCGACCTCTATCAACTGTTTCGCAACTGTTCACTTGCCGTACTGAACTCCGGTAGTTTGACCGATAACAGCAAAGAATTGCGTTCTCGTTGTGAAAATTTCGATATTAACTTCTTGCGCCGTg                       <  2:952793/250‑1 (MQ=255)                                                                                                                                                                                                                           tGTAATCAAAGGGAATGACGATATTCGTCTCATAAGGAGTTTT‑TCTTGATTACACATATTAGCCCGCTTGGCTCCATGTA‑TA‑GTTGGTC‑GCTGCTGGAAGTGTATATGCTGAAACGCACCGCCAGCAGCGACCTCTATCAACTGTTTCGCAACTGTTCACTTGCCGTACTGAACTCCGGTAGTTTGACCGATAACAGCAAAGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTg                       <  1:614282/250‑1 (MQ=255)                                                                                                                                                                                                                           tGTAATCAAAGGGAATGACGATATTCGTCTCATAAGGAGTTTT‑TCTTGATTACACATATTAGCCCGCTTGGCTCCATGGA‑TATGTGG‑TC‑GCAGCTGGAAGTGGATATGCTTAAACGCACCGCCAGCAGCGACCTCTATCAACTGTTTCGCAACTGTTCACTTGCCGTACTGAACTCCGGTAGTTTGACCGATAACAGCAAAGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTg                       <  1:918416/250‑1 (MQ=255)                                                                                                                                                                                                                              aataaaaGGGAATGACGATATTCGGCTCATAGTGAGTTTT‑TTTTGATTACACATATTAGCCCGCTTGGCTCCATGGA‑TATGTTG‑TC‑GCAGCTGGAAGTGGATATGCTTAAACGCACCGCCAGCAGCGACCTCTATCAACTGTTTCGCAACTGTTCACTTGCCGTACTGAACTCCGGTAGTTTGACCGATAACAGCAAAGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAAc                    <  2:127987/246‑1 (MQ=255)                                                                                                                                                                                                                              aaTCAAAGGAAATGACGATTTTCGTCTAATAAGGGTTTTT‑TCTTGATTACACATTTTAGCCCGCTTGGCTCCAGGGA‑TATGTGG‑TC‑GCAGCTGGAAGTGGATAGGCTTAAACGCACCGCCAGCAGCGACCTCTATCAACTGTTTCGCAACTGTTCACTTGCCGTACTGAACTCCGGTAGTTTGACCGATAACAGCAAAGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAAcg                   <  2:839721/251‑1 (MQ=255)                                                                                                                                                                                                                                tCAAAGGGAATGTCGATATTCGACTCATAAGGAGTTTA‑TCTTGATTACACGAAATAGCACGCTTGGCTACATGGA‑TAAGTTG‑TA‑GCCGCTGGAAGAGAAAATGCTAAAACGCACCGCAAGCAGCGACATCTATCAACTGTTGCGCAACTGTTCACTTGCCGTACTGAACTCCGGTAGTTTGACCGATAACAGCAAAGAATTGCTGTCTCGTTTTGAAAATTTAGATATTAACGACTTGAGCAGTGAAcgcg                 >  2:621455/1‑251 (MQ=255)                                                                                                                                                                                                                                aaaaaGGGAATGTCTATATTCGGCTAATAAG‑TGTTTTGTCTTGATTCCATATATTAGCCCTCTTGGCCCCTGGGA‑TTGGTAG‑TC‑GCAGTTGGAATTGGATATGCTTAAACGCACCGCCATCAGCGACCTCTATCAACTGTTTTGCAACTGTTCACTTGCCGTACTGAACGCCGGTAGTTTGACCGATAACAGCAAAGAATGGCTGTCTCGTTTTGAAAATTTCGATAGTAACGTCTTGCGCCGTGAAcgcg                 <  2:599497/249‑1 (MQ=255)                                                                                                                                                                                                                                 cAAAGGGAATGACGATATTTGTCTCATAAGGAGTTTT‑TCTTGCTGACACATATTAGCCCTCTTGGCTCCATGGA‑TAGGTTG‑TC‑GCAGCTGGAAGTGGTTATGCTTAAACGCACCGCCAGCAGCGACCTCTATCAACTGTTTCGCAACTGTTCACTTGCCGTACTGAACTCCGGTAGTTTGACCGATAACAGCAAAGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACgcgg                <  1:851231/251‑1 (MQ=255)                                                                                                                                                                                                                                  aaaGGTAATGACTATGTGCGTCTCAAAAGGAGTTTT‑TTCTGAATACACATATTGCCCCGCTTGCCCCCATGAA‑TGTGTGT‑TC‑GCAGCTGGAAGTGGATATGACTAATCGCACCGCCAGCAGCGACCTCTATCAACTGTTTCGCAACTGTTCACTTGCCGTACTGAACACCGGTAGTTTGACCGATAACAGCAAAGAATTGCTGTCTCGTGTTGAAAATTGCGATATTAACGTCTTGCGCCGTGAACgcggc               <  2:487392/251‑1 (MQ=255)                                                                                                                                                                                                                                       gAATGACGATATTCGTCTCATAAGGAGTTTT‑TCTTGATTACACATATTAGCCCGCTTGGCTCCATGGA‑TATGTTG‑TC‑GCTGCTGGAAGTGGATATGCTTAAACGCACCGCCAGCAGCGACCTCTATCAACTGTTACGCAACTGTTCACTTGCCGTACTGAACTCCGGTAGTTTGACCGATAACAGCAAAGAATTGCTGTCTAGTTTTGAAAGTTACGATATAAAGGTCTTGCGCCGTGAACGCGGCGTaa           >  2:774433/1‑250 (MQ=255)                                                                                                                                                                                                                                               atatTCTTCTCAAAAGGAGTTTT‑TCTTGATTACACATATTAGCCCGCTTGGCTCCATGGA‑TATGTTG‑TC‑GCAGCTGGAAGTGGATATGCTTAAACGCTCCGCCAGCAGCGACCTCTATCAACTGTTTCGCAACTGTTCACTTGCCGTACTGAACTCCGGTAGTTTGACCGATAACAGCAAAGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGaa   <  1:149402/250‑1 (MQ=255)                                                                                                                                                                                                                                               atatTCGTCTCATAAGGATTTTT‑TCTTGATTACACATATTAGCCCGCTTGGCTCCCTGGA‑TATGTTG‑TC‑GCAGCTGGAAGTGGATATGCTTAAACGCACCGCCAGCAGCGACCTCTATCAACTGTTTCGCAACTGTTCACTTGCCGTACTGAACTCCGGTAGTTTGACCGATAACAGCAAAGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAAc  <  1:108932/251‑1 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                      GAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGCGGTCTGGACATTAACCCGTGGCGCAGTAATAGCGATTTTGTCCCATCGACCACAAGACTGGTTCGGCAATAAATTTTTTCT‑CAATTTTGCGTGCTGGATTCACGCAGAAGGTTGTGAAAGGTCATCAGGC‑AGGGCTATTGTAATCAAAGGGAATGACGATATTCGTCCCATAAGGAGTTTT‑TCTTGATTACACATATTAGCCCGCTTGGCTCCATGGA‑TATGTTG‑TC‑GCAGCTGGAAGTGGATATGCTTAAACGCACCGCCAGCAGCGACCTCTATCAACTGTTTCGCAACTGTTCACTTGCCGTACTGAACTCCGGTAGTTTGACCGATAACAGCAAAGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAAC  >  NC_000913/2926047‑2926533

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑