Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,384,808	C→T	K305K (AAG→AAA)	yfbK ←	IPR002035/DUF3520 domain‑containing protein YfbK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,384,808	0	C	T	95.7%	266.2 / 0.3	95	K305K (AAG→AAA)	yfbK	IPR002035/DUF3520 domain‑containing protein YfbK
Reads supporting (aligned to +/- strand):  ref base C (0/4);  new base T (56/32);  total (56/39)
Fisher's exact test for biased strand distribution p-value = 2.11e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.67e-01

ATACTTTCTGCGCTTCAGAGAGGGTATCAATGTAGCTGTAGTTGCCGTTACCAACATCGGCAATTCGCACCATCATTGCCTCGTTGTAATTGCTATTCCCCACGCCAAACGTCGACAGAGTAACACCAGACTCCCGCTGTTTTTTGACCATTGATTCAATCGATTTTGGA‑TCGTCA‑ATGCCAACGTTAAAGTCACCGTCAGTGGC‑TAATAAAATG‑CGATTGATGCCGCCCTTAATAAACCCCTT‑C‑G‑T‑CGCCTGCTGATAAGCCAGTTCCAGCCCGGCACCGCCATTGGTACTGCCTTCGGCATCCAGCGAATCAATTGCGGCATTAATTTCCGCCTTATGACTCCCGGAGATAGAAGGCAATGCAATACGGGAGTCGCCAGCGTAGGTCACGATGGCAATGTTATCCTGCTCACGAAGTTCTTTGACCAATAATTTCAACGAAGACTGGATAAGTGGCAAACGTTCATCAGAAATCATTGAACCAGA  >  NC_000913/2384567‑2385053                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                          agaCTTTCTGCGCTTCAGAGAGGGTATCAATGTAGCTGTAGTTGCCGTTACCAACATCGGCAATTCGCACCATCATTGCCTCGTTGTAATTGCTATTCCCCACGCCAAACGTCGACAGAGTAACACCAGACTCCCGCTGTTTTTTGACCATTGATTCAATCGATTTTGGA‑TCGTCA‑ATGCCAACGTTAAAGTCACCGTCAGTGGC‑TAATAAAATG‑CGATTGATGCCGCCCTTAATAAACCCTTT‑C‑G‑T‑CGcc                                                                                                                                                                                                                                              <  1:110511/249‑1 (MQ=255) aTACTTTCTGCGCTTCAGAGAGGGTATCAATGTCGCTGTAGATGCCGTAACCAACATAGGCCATTAGCACCATCATTGCCTCGTTGTAATTGCTATTCCCCACGCCAAACGTCGACAGAGTAACACACGACTCCCGCAGTTTTTTGACCATTGATTCAATCACTTTTGGA‑TCGTCA‑ATGCCCACGTTCAAGTCACAGTCCGTGGC‑TAATAAAATG‑GGATTGATGCCGCCCTTAATAAACCCTTT‑C‑C‑T‑CGcc                                                                                                                                                                                                                                              >  2:178255/1‑251 (MQ=255) aTACTTTCTGCGCTTCAGAGAGGGTATCAATGTAGCTGTAGTTGCCGTTACCAACATCGGCAATTCGCACCATCATTGCCTCGTTGTAATTGCTATTCCCCACGCCAAACGTCGACAGAGTAACACCAGACTCCCGCTGTTTTTTGACCATTGATTCAATCGATTTTGGA‑TCGTCA‑ATGCCAACGTTAAAGTCACCGTCAGTGGC‑TAATAAAATG‑CGATTGATGCCGCCCTTAATAAACCCTGT‑C‑G‑T‑CGcc                                                                                                                                                                                                                                              <  2:475801/251‑1 (MQ=255)  tACTTTCTGCGCTTCAGAGAGGGTATCAATGTAGCTGTAGTTGCCGTTACCAACATCGGCAATTCGCACCATCATTGCCTCGTTGTAATTGCTATTCCCCACGCCAAACGTCGACAGAGTAACACCAGACTCCCGCTGTTTTTTGACCATTGATTCAATCGATTTTGGA‑TCGTCA‑ATGCCAACGTTAAAGTCACCGTCAGTGGC‑TAATAAAATG‑CGATTGATGCCGCCCTTAATAAACCCTTT‑C‑G‑T‑CGCct                                                                                                                                                                                                                                             >  1:691701/1‑251 (MQ=255)      ttCTGCGCTTCAGAGAGGGTATCAATGTAGCTGGAGTTGCCGTTACCAACAACGGCAATTCGCACCAACATTGCCTCGTTGTAATTGCTATTCCCCACGCCAAACGTCGACAGAGTAACACCAGACTCCCGCTGTTTTTTGACCATTGATTCAAGAGATTTTGGA‑TCGTCA‑ATGCCAACGTTAAAGTCACCGTCAGTGGC‑TAATAAAATG‑CGATGGATGCCGCCCTGAATAAACCCTTT‑C‑G‑T‑CGCctgct                                                                                                                                                                                                                                          <  2:108737/250‑1 (MQ=255)       tCTGCGCTTCAGAGAGGGTATCAATGTAGCTGTAGTTGCCGTTACCAACATCGGCAATTCGCACCATCATTGCCTCGTTGTAATTGCTATTCCCCACGCCAAACGTAGACAGAGTAACACCAGACTCCCGCTGTTTTTTGACCATTGATTCAATCGATTTTGGA‑TCGTCA‑ATGCCAACGTTAAAGTCACCGTCAATGGC‑TAATAAAATG‑CGATTGATGCCGCCCTTAATAAACCCTTT‑C‑G‑T‑CGCcttctg                                                                                                                                                                                                                                         >  2:29321/1‑250 (MQ=255)       tCTGCGCTTCAGAGAGGGTAGCAATGTAGCTGTAGTTGCCGTTACCAACATCGGCAATTCGCACCATCATTGCCTCGTTGTAATTGCTATTCCCCACGCCAAACGTCGACAGAGTAACACCAGACTCCCGCTGTTTTTTGACCATTGATTCAATCGATTTTGGA‑TCGTCA‑ATGCCAACGTTAAAGTCACCGTCAGTGGC‑TAATAAAATG‑CGATTGATGCCGCCCTTAATAAACCCTTT‑C‑G‑T‑CGCctgctg                                                                                                                                                                                                                                         <  2:468874/250‑1 (MQ=255)         cgcgcTTCAGAGAGGGTATCAATGTAGCTGTAGTTGCCGTTACCAACATCGGCAATTCGCACCATCATTGCCTCGTTGTAATTGCTATTCCCCACGCCAAACGTCGTCAGAGTAACACCAGACTCCCGCTGTTTTTTGACCATTGATTCAATCGATTTTGGA‑TCGTCA‑ATGCCAACGTTAAAGTCACCGTCAGTGAC‑TATTAACATG‑CGATAGTGGCCGCCCTTAATAAACCTTTt‑c‑g‑t‑cactggctgat                                                                                                                                                                                                                                       >  2:871235/2‑240 (MQ=255)          gcgcTTCAGAGAGGGGATCAATGTAGCTGTAGTTGCCGTTACCAACATCGGCAATTCGCACCATCATTGCCTCGTTGTAATTGCTATTCCCCACGCCAAACGTCGACAGAGTAACACCAGACTCCCGCTGTTTTTTGACCATTGATTCAATCGATTTTGGA‑TCGTCA‑ATGCCAACGTTAAAGTCACCGTCAGTGGC‑TAATAAAATG‑CGATTGATGCCGCCCTTAATAAACCCTTT‑C‑G‑T‑CGCCTGCTGATaa                                                                                                                                                                                                                                     <  1:834882/251‑1 (MQ=255)           cgcTTCAGAGAGGGTATCAATGTAGCTGTAGTTGCCGTTACCAACATCGGCAATTCGCACCATCATTGCCTCGTTGTAATTGCTATTCCCCACGCCAAACGTCGACAGAGTAACACCAGACTCCCGCTGTTTTTTGACCATTGATTCAATCGATTTTGGA‑TCGTCA‑ATGCCAACGTTAAAGTCACCGTCAGTGGC‑TAATAAAATG‑CGATTGATGCCGCCCTTAATAAACCCTTT‑C‑G‑T‑CGCCTGCTGATa                                                                                                                                                                                                                                      >  1:149929/1‑249 (MQ=255)             cTTCAGAGAGGGTATCAATGTAGCTGTAGTTGCCGTTACCAACATCGGCAATTCGCACCATCATTGCCTCGTTGTAATTGCTATTCCCCACGCCAAACGTCGACAGAGTAACACCAGACTCCCGCTGTTTTTTGACCATTGATTCAATCGATTTTGGA‑TCGTCA‑ATGCCAACGTTAAAGTCACCGTCAGTGGC‑TAATAAAATG‑CGATTGATGCCGCCCTTAATAAACCCTTT‑C‑G‑T‑CGCCTGCTGATAAGcc                                                                                                                                                                                                                                  >  1:686559/1‑251 (MQ=255)              ttCAGAGAGGGTATCAATGTAGCTGTAGTTGCCGTTACCAACATCGGCAATTCGCACCATCATTGCCTCGTTGTAATTGCTATTCCCCACGCCAAACGTCGACAGAGTAACACCAGACTCCCGCTGTTTTTTGACCATTGATTCAATCGATTTTGGA‑TCGTCA‑ATGCCAACGTTAAAGTCACCGTCAGTGGC‑TAATAAAATG‑CGATTGATGCCGCCCTTAATAAACCCTTT‑C‑G‑T‑CGCCTGCTGATAAGcc                                                                                                                                                                                                                                  >  2:332878/1‑250 (MQ=255)               tCAGAGAGGGTATCAATGTAGCTGTAGTTGCCGTTACCAACATCGGCAATTCGCACCATCATTGCCTCGTTGTAATTGCTATTCCCCACGCCAAACGTCGACAGAGTAACACCAGACTCCCGCTGTTTTTTGACCATTGATTCAATCGATTTTGGA‑TCGTCA‑ATGCCAACGTTAAAGTCACCGTCAGTGGC‑TAATAAAATG‑CGATTGATGCCGCCCTTAATAAACCCTTT‑C‑G‑T‑CGCCTGCTGATAAGcc                                                                                                                                                                                                                                  >  1:494966/1‑249 (MQ=255)                cAGAGAGGGGATCAATGTAGCTGTAGTTGCCGTTACCAACATCGGCAATTCGCACCATCATTGCCTCGTTGTAATTGCTATTCCCCACGCCAAACGTCGACCGAGTAACACCAGAATCCCGCTGTTTTTTGCCCATTGATTCAATCGATTTTGGA‑TCGTCG‑CTGCCAACGTTAAAGTCACCGTCAGTGGC‑TAATAAAATG‑CGATTGATGCCGCCCTTAATAACACCTTT‑A‑G‑T‑CGCCTGCTGCTAAGCCAGt                                                                                                                                                                                                                               >  2:837211/1‑251 (MQ=255)                 agagagGGTATCAATGTAGCTGTAGTTGCCGTTACCAACATCGGCAATTCGCACCATCATTGCCTCGTTGTAATTGCTATTCCCCACGCCAAACGTCGACAGAGTAACACCAGACTCCCGCTGTTTTTTGACCATTGATTCAATCGATTTTGGA‑TCGTCA‑ATGCCAACGTTAAAGTCACCGTCAGTGGC‑TAATAAAATG‑CGATTGATGCCGCCCTTAATAAACCCTTT‑C‑G‑T‑CGCCTGCTGATAAGCCAGtt                                                                                                                                                                                                                              >  1:829783/1‑251 (MQ=255)                 agagagGGTATAAATGTAGCTGTAGTTGCCGTTACCAACATCGGCAATTCGCACCATCATTGCCTCGTTGTAATTGCTATTCCCCACGCCAAACGTCGACAGAGTAACACCAGACTCCCGCTGTTTTTTGACCATTGATTCAATCGATTTTGGA‑TCGTCA‑ATGCCAACGTTAAAGTCACCGTCAGTGGC‑TAATAAAATG‑CGATTGATGCCGCCCTTAATAAACCCTTT‑C‑G‑T‑CGCCTGCTGATAAGCCAGtt                                                                                                                                                                                                                              <  2:683556/251‑1 (MQ=255)                  gagagGGTATCAATGTAGCTGTAGTTGCCGTTACCAACATCGGCAATTCGCACCATCATTGCCTCGTTGTAATTGCTATTCCCCACGCCAAACGTCGGCAGAGTAACACCAGACTCCCGCTGTTTTTTGACCATTGATTCAATCGATTTTGGA‑TCGTCA‑ATGCCAACGTTAAAGTCACCGTCAGTGGC‑TAATAAAATG‑CGATTGATGCCGCCCTTAATAAACCCTTT‑C‑G‑T‑CGCCTGCTGATAAGCCAGTTc                                                                                                                                                                                                                             >  1:617362/1‑251 (MQ=255)                  gagagGGTATCAATGTAGCTGTAGTTGCCGTTACCAACATCGGCAATTCGCACCATCATTGCCTCGTTGTAATTGCTATTCCCCACGCCAAACGTCGACAGAGTAACACCAGACTCCCGCTGTTTTTTGACCATTGATTCAATCGATTTTGGA‑TCGTCA‑ATGCCAACGTTAAAGTCACCGTCAGTGGC‑TAATAAAATG‑CGATTGATGCCGCCCTTAATAAACCCTTT‑C‑G‑T‑CGCCTGCTGATAAGCCAGTTc                                                                                                                                                                                                                             >  2:372828/1‑251 (MQ=255)                    gagGGTATCAATGTAGCTGTAGTTGCCGTTACCAACATCGGCAATTCGCACCATCATTGCCTCGTTGTAATTGCTATTCCCCACGCCAAACGTCGACAGAGTAACACCAGACTCCCGCTGTTTTTTGACCATTGATTCAATCGATTTTGGA‑TCGTCA‑ATGCCAACGTTAAAGTCACCGTCGGTGGC‑TAATAAAATG‑CGATTGATGCCGCCCTTAATAAACCCTTT‑C‑G‑T‑CGCCTGCTGATAAGCCAGTTCCa                                                                                                                                                                                                                           <  1:538470/251‑1 (MQ=255)                          aTCAATGTAGCTGTAGTTGCCGTTACCAACATCGGCAATTCGCACCATCATTGCCTCGTTGTAATTGCTATTCCCCACGCCAAACGTCGACAGAGTAACACCAGACTCCCGCTGTTTTTTGACCATTGATTCAATCGATTTTTGA‑TCGTCA‑GTGCCAACGTTAAATTCACCGTCAGTGGC‑TAATAAAATG‑CGATTGATGCCGCCCTTAATAAACCCTTT‑C‑G‑T‑CGCCTGCTGATAAGCCAGTTCCAGCCCg                                                                                                                                                                                                                      <  1:40473/250‑1 (MQ=255)                          aTCAATGTAGCTGTAGTTGCCGGTACCAACAGCGGCAATTCGCACCATCATTGCCTCGTTGTAATTGCTATTCCCCCCGCCAAACGTCGACAGAGTAACACCAGACTCCCGCTGTTTTTTGACCATTGATTCAATCGATTTTGGA‑TCGTCA‑ATGCCAACGTTAAAGTCACCGTCAGTGGC‑TAATAAAATG‑CGATTGATGCCGCCCTTAATAAACCCTTT‑C‑G‑T‑CGCCTGCTGATAAGCCAGTTCCAGCCCg                                                                                                                                                                                                                      <  1:13039/250‑1 (MQ=255)                            cAATGTAGCTGTAGTTGCCGTTACCAACATCGGCAATTCGCACCATCATTGCCTCGTTGTAATTGCTATTCCCCACGCCAAACGTCGACAGAGTAACACCAGACTCCCGCTGTTTTTTGACCATTGATTCAATCGATTTTGGA‑TCGTCA‑ATGCCAACGTTAAAGTCACCGTCAGTGGC‑TAATAAAATG‑CGATTGATGCCGCCCTTAATAAACCCTTT‑C‑G‑T‑CGCCTGCTGATAAGCCAGTTCCAGCCCGGc                                                                                                                                                                                                                    >  2:4597/1‑250 (MQ=255)                                gTAGCTGTAGTTGCCGTTACCAACATCGGCAATTCGCACCATCATTGCCTCGTTGTAATTGCTATTCCCCACGCCAAACGTCGACAGAGTAACACCAGACTCCCGCTGTTTTTTGACCATTGATTCAATCGATTTTGGA‑TCGTCA‑ATGCCAACGTTAAAGTCACCGTCAGTGGC‑TAATAAAATG‑CGATTGATGCCGCCCTTAATAAACCCTTT‑C‑G‑T‑CGCCTGCTGATAAGCCAGTTCCAGCCCGGCACCg                                                                                                                                                                                                                <  1:215233/250‑1 (MQ=255)                                     tGTAGTTGCCGTTACCAACATCGGCAATTCGCACCATCATTGCCTCGTTGTAATTGCTATTCCCCACGCCAAACGTCGACAGAGTAACACCAGACTCCCGCTGTTTTTTGACCATTGATTCAATCGATTTTGGA‑TCGTCA‑ATGCCAACGTTAAAGTCACCGTCAGTGGC‑TAATAAAATG‑CGATTGATGCCGCCCTTAATAAACCCTTT‑C‑G‑T‑CGCCTGCTGATAAGCCAGTTCCAGCCCggacccgcccttg                                                                                                                                                                                                          >  2:106628/1‑240 (MQ=255)                                         gTTGCCGTTACCAACATCGGCAATTCGCACCATCATTGCCTCGTTGTAATTGCTATTCCCCACGCCAAACGTCGACAGAGTAACACCAGACTCCCGCTGTTTTTTGACCATTGATTCAATCGATTTTGGA‑TCGTCA‑ATGCCAACGTTAAAGTCACCGTCAGTGGC‑TAATAAAATG‑CGATTGATGCCGCCCTTAATAAACCCTTT‑C‑G‑T‑CGCCTGCTGATAAGCCAGTTCCAGCCCGGCACCGCCATTGGTAc                                                                                                                                                                                                      >  1:705475/1‑251 (MQ=255)                                                  aCCAACATCGGCAATTCGCACCATCATTGCCTCGTTGTAATTGCTATTCCCCACGCCAAACGTCGACAGAGTAACACCAGACTCCCGCTGTTTTTTGACCATTGATTCAATCGATTTTGGA‑TCGTCA‑ATGCCAACGTTAAAGTCACCGTCAGTGGC‑TAATAAAATG‑CGATTGATGCCGCCCTTAATAAACCCTTT‑C‑G‑T‑CGCCTGCTGATAAGCCAGTTCCAGCCCGGCACCGCCATTGGTACTGCCTTCgg                                                                                                                                                                                             >  1:28551/1‑251 (MQ=255)                                                   ccAACATCGGCAATTCGCACCATCATTGCCTCGTTGTAATTGCTATTCCCCACGCCAAACGTCGACAGAGTAACACCAGACTCCCGCTGTTTTTTGACCATTGATTCAATCGATTTTGGA‑TCGTCA‑ATGCCAACGTTAAAGTCACCGTCAGTGGC‑TAATAAAATG‑CGATTGATGCCGCCCTTAATAAACCCTTT‑C‑G‑T‑CGCCTGCTGATAAGCCAGTTCCAGCCCGGCACCGCCATTGGTACTGCCTTCGGc                                                                                                                                                                                            >  1:659780/1‑251 (MQ=255)                                                      aCATCGGCAATTCGCACCATCATTGCCTCGTTGTAATTGCTATTCCCCACGCCAAACGTCGACAGAGTAACACCAGACTCCCGCTGTTTTTTGACCATTGATTCAATCGATTTTGGA‑TCGTCA‑ATGCCAACGTTAAAGTCACCGTCAGTGGC‑TAATAAAATG‑CGATTGATGCCGCCCTTAATAAACCCTTT‑C‑G‑T‑CGCCTGCTGATAAGCCCGTTCCAGCCCGGCACCGCCATTGGTACTGCCTTCGGCATc                                                                                                                                                                                         >  2:291884/1‑251 (MQ=255)                                                             cAATTCGCACCATCATTGCCTCGTTGTAATTGCTATTCCCCACGCCAAACGTCGACAGAGTAACACCAGACTCCCGCTGTTTTTTGACCATTGATTCAATCGATTTTGGA‑TCGTCA‑ATGCCAACGTTAAAGTCACCGTCAGTGGC‑TAATAAAATG‑CGATTGATGCCGCCCTTAATAAACCCTTT‑C‑G‑T‑CGCCTGCTGATAAGCCAGTTCCAGCCCGGCACCGCCATTGGTACTGCCTTCGGCATCCAGCGa                                                                                                                                                                                   >  1:529619/1‑250 (MQ=255)                                                                 tctcactaccatggCCTCGTTGTAATTGCTATTCCCCACGCCAATCGTCCACATAGTAACACCAGCCCCCCGCTGTGTTTGTACCATTTATTAAACCTATTTGGGT‑TCGTAA‑ATGCCAACTTTAAAGTCACCG‑CGATGGCCTAATAAAGTG‑CGTTTGAGGCCGCCCTGAATAAACCC‑TT‑C‑C‑TGCGCTTGCGGATAAGCTCGTCCCATCCCGGCCCCGCCATTGGTACTGCCTTCGTCATCCAGCTAATCa                                                                                                                                                                               <  2:648369/237‑1 (MQ=255)                                                                  cGCACCATCATTGCCTCGTTGTAATTGCTATTCCCCACGCCAAACGTCGACAGAGTAACACCAGACTCCCGCTGTTTTTTGACCATTGATTCAATCGATTTTGAA‑TCGTCC‑ATGCCAACGTTAAAGTCACCGTCAGTGGC‑TAATAAAATG‑CGATTGATGCCGCCCTTAATAAAACCTTT‑C‑G‑T‑CGACAGCTGATAAGCCAGTTCCAGCCCGGAACCGCCATTGGTACTGCCTTCGGAATCCAGCGAATCAAt                                                                                                                                                                             >  1:878970/1‑251 (MQ=255)                                                                   gCACCATCATTGCCTCGTTGTAATTGCTATTCCCCACGCCAAACGTCGACAGAGTAACACCAGACTCCCGCTGTTTTTTGACCATTGATTCAATCGATTTTGGA‑TCGTCA‑ATGCCAACGTTAAAGTCACCGTCAGTGGC‑TAATAAAATG‑CGATTGATGCCGCCCTTAATAAACCCTTT‑C‑G‑T‑CGCCTGCTGATAAGCCAGTTCCAGCCCGGCACCGCCATTGGTACTGCCTTCGGCATCCAGCGAATCAAtt                                                                                                                                                                            >  2:260254/1‑251 (MQ=255)                                                                      acatcatTGCCTCGTTGTAATTGCTATTCCCCACGCCACACGTCGACAGAGTAACACCAGACTCCCGCTGTTTTTTGACCATTGATTCAATCGATTTTGGA‑TCGTCA‑ATGCCAACGTTAAAGTCACCGTCAGTGGC‑TAATAAAATG‑CGATTGATGCCGCCCTTAATAAACCCTTT‑C‑G‑T‑CGCCTGCTGATAAGCCAGTTCCAGCCCAGCACCGCCATTGGTCCTTCCTTCGACATCCAGCGAATCCATTGCg                                                                                                                                                                         >  2:536163/2‑251 (MQ=255)                                                                         tcatTGCCTCGTTGTAATTGCTATTCCCCACGCCAAACGTCGACAGAGTAACACCAGACTCCCGCTGTTTTTTGACCATTGATTCAATCGATTTTGGA‑TCGTCA‑ATGCCAACGTTAAAGTCACCGTCAGTGGC‑TAATAAAATG‑CGATTGATGCCGCCCTTAATAAACCCTTT‑C‑G‑T‑CGCCTGCTGATAAGCCAGTTCCAGCCCGGCACCGCCATTGGTACTGCCTTCGGCATCCAGCGAATCAATTGCGGc                                                                                                                                                                       >  1:265008/1‑250 (MQ=255)                                                                          catgcccTCGTTGTAATTGCTATTCCCCTCGCCAAACGTCGACAGAGTAACACCAGACTCCCGCTTTTTTTTGCCCATTGATTCAATCGATTTTGGA‑TCGTCA‑ATGCCAACGTTAAAGTCACCGTCAGTGGC‑TAATAAAATG‑CGATTGATGCCGCCCTTAATAACCCCTTT‑C‑G‑T‑CGCCTGCTGATAAGCCAGTCCCAGCCCGGCACCGCCATTGGTACTGCCTTCGGCATCCAGCGAATCAATTGCGGCAt                                                                                                                                                                     <  1:81821/246‑1 (MQ=255)                                                                            ttGCCTCGTTGTAATTGCTATTCCCAACGCCAAACGTCGACAGAGTAACACCAGGCTCCCGCTGTTTTTTGACCATTGATTCAATCGATTTTGGA‑TCGTCA‑ATGCCAACGTTAAAATCACCGTCAGTGGC‑TAATAAAAGG‑CGATTGATGCCGCCCTTAATAAACCCTTT‑C‑G‑T‑CGCATGCTGATAAGACAGTTCCAGCCCGGCCCCGCCATTGGAACTGCCTCCGGCAGCCCGCGAATACATTGCGGCAttc                                                                                                                                                                   >  2:284912/1‑250 (MQ=255)                                                                            ttGCCCCGTTTTAATTGCTATTCCCCACGCCAAACGTCGACAGAGAAACACGAGACTCCCGCTGTTTTTTGACCATTGATTCAATCGATGTTGGA‑TCGTCA‑ATGCCAGCGTTAAAGTCACCGTCGGTGGC‑AAATAAAATG‑CGATTGAGGCCGCCCTTAATAACCCCTTT‑C‑G‑T‑CGCCTGCTGATAAGCCATTTCCAGCCCGGCGCCGCCATTGGTACTGCCTTCGGCATCCAGCGAATCAATTGCGGCAtt                                                                                                                                                                    <  1:370266/250‑1 (MQ=255)                                                                                  cGTTGTAATTGCTATTCCCCACGCCAAACGTCGACAGAGTAACCCCAGACTCCCGCTGTTTTTTGACCATTGATTCAATCGATTTTGGA‑TCGTCA‑ATGCCAACGTTAAAGTCACCGTCAGTGGC‑TAATAAAATG‑CGATTGATGCCGCCCTTAATAAACCCTTT‑C‑G‑T‑CGCCTGCTGATAAGCCAGTTCCAGCCCGGCACCGCCATTGGTACTGCCTTCGGCATCCAGCGAATCAATTGCGGCATTAATTTc                                                                                                                                                              <  1:298512/250‑1 (MQ=255)                                                                                              tATTCCCCACGCCAAACGTCGACAGAGTAACACCAGACTCCCGCTGTTTTTTGACCATTGATTCAATCGATTTTGGA‑TCGTCA‑ATGCCAACGTTAAAGTCAACGTCAGTGGC‑TAATAAAATG‑CGATTGATGCCGCCCTTAATAAACCCTTT‑C‑G‑T‑CGCCTGCTGATAAGCCAGTTCCAGCCCGGCACCGCCATTGGTACTGCCTTCGGCATCCAGCGAATCAATTGCGGCATTAATTTCCGCCTTATGACTc                                                                                                                                                 >  1:885130/1‑251 (MQ=255)                                                                                                 tCCCCACGCCAAACGTCGACAGAGTAACACCAGACTCCCGCTGTTTTTTGACCATTGATTCAATCGATTTTGGA‑TCGTCA‑ATGCCAACGTTAAAGTCACCGTCAGTGGC‑TAATAAAATG‑CGATTGATGCCGCCCTTAATAACCCCTTT‑C‑G‑T‑CGCCTGCTGATACGCCAGTTCCAGCCCGGCACCGCCATTGGTACTGCCTTCGGCATCCAGCGAATCAATTGCGGCCTTAATTTCCGCCttctgcatcccg                                                                                                                                              >  2:503660/1‑241 (MQ=255)                                                                                                       cgccaaccttcacaggagTAACACAAGACTCCCTCTTTTTTTTGACCATTTATTCATTCGATTTGGAA‑ACTTCA‑ATGCCAACGTTAAAGTCACCGCCAGGGGC‑TAATAAATTG‑CGATTGATGCCGCCCCTAATAAACCCTTT‑C‑G‑T‑CGCTTGCTAATAAGCCAGTTCCAGCCCGGCACCGCCATTGGTACTGCCTTCGGCATCCAGCGAATCAATTGCGGCATTAATTTCCGCCTTATGACTCCCGGAGATa                                                                                                                                        <  2:149193/236‑1 (MQ=255)                                                                                                         ccAAACGTCGACAGAGTAACACCAGACTCCCGCTGTTTTTTGACCATTGATTCAATCGATTTTGGA‑TCGTCA‑ATGCCAACGTTAAAGTCACCGTCAGTGGC‑TAATAAAATG‑CGATTGATGCCGCCCTTAATAAACCCTTT‑C‑G‑T‑CGCCTGCTGATAAGCCAGTTCCAGCCCGGCACCGCCATTGGTACTGCCTTCGGCATCCAGCGAATCAATTGCGGCATTAATTTCCGCCTTATGACTCCCGGAGAt                                                                                                                                         >  1:790204/1‑248 (MQ=255)                                                                                                          cAAACGTCGACAGAGTAACACCAGACTCCCGCTGTTTTTTGACCATTGATTCAATCGATTTTGGA‑TCGTCA‑ATGCCAACGTTAAAGTCACCGTCAGTGGC‑TAATAAAATG‑CGATTGATGCCGCCCTTAATAAACCCTTT‑C‑G‑T‑CGCCTGCTGATAAGCCAGTTCCAGCCCGGCACCGCCATTTGTACTGCCTTCGGCATCCAGCGAATCAATTGCGGCATTAATTTCCGCCTTATGACTCCCGGAGATAGa                                                                                                                                      >  1:473073/1‑250 (MQ=255)                                                                                                           atacgccgaaagattaacccctgccTCCCTCTGTTTTTTGACCTTTGATTAAACCGATTGTGGA‑CGGTCA‑ATGCCAACTTTAAATTCACCGTCAGTGGT‑TAATAAAAGG‑CGATTGAGGCCCCCCTTAATAATCCCTTC‑C‑G‑T‑CGCCTGCTGAAAGGCCATTTCCAGCCGGTCACCGCCATTGGTACTGCCTTCGGCATCCAGCGAATCAATTGCGGCATTAATTTCCGCCTTATGACTCCCGTATATAGAAg                                                                                                                                    <  2:915765/227‑1 (MQ=255)                                                                                                               gttgatagagTAACACCAGCCTCCCGTTGTTTTTTGCCCATTGATTCACTCGTTTTTGGA‑TCGTCA‑ATGCAAACGTTAAATTCCCCGTCAGTGGC‑TAATAAAATG‑CGATTGATGCCGCCCTTACTAAACCCTTT‑C‑G‑T‑CGCCTTCTGATAAGCCAGTTCCAGCCCGGCACCGCCATTGGTACTGCCTTCGGCATCCAGCGAATCAATTGCTGCATTAATTTCCGCCTTATGACTCCCGGAGATAGAAGgcaa                                                                                                                                <  1:648691/245‑1 (MQ=255)                                                                                                                   acatataaaccccaaacccccGTTGTTTTTTGACCATTTATTAAATCGATTGTGGA‑TGGCTA‑ATGCCAACGTGAAATTCACCGTCAGTGGC‑TAATAAAATG‑CGATTGAGGCCGCCCTAAATAACCCCTTT‑C‑G‑T‑CGCCGGCGGAAAAGCCTGTTCCAGCCCGGCACCGCCATTGGTACTGCCTTCGGCATCCAGCGAATCAATTGCGGCATTAATTTCCGCCTTATGACTCCCGGAGATAGAAGgcaatgca                                                                                                                            <  2:426012/233‑1 (MQ=255)                                                                                                                     agagTAACACCAGACTCCCGCTGTTTTTTGACCATTGATTCAATCGATTTTGGA‑TCGTCA‑ATGCCAACGTTAAAGTCACCGTCAGTGGC‑TAATAAAATG‑CGATTGATGCCGCCCTTAATAAACCCTTT‑C‑G‑T‑CGCCTGCTGATAAGCCAGTTCCAGCCCGGCACCGCCATTGGTACTGCCTTCGGCATCCGGCGAATCAATTGCGGCATTAATTTCCGCCTTATGACTCCCGGAGATAGAAGgcaatgcaa                                                                                                                           >  1:520126/1‑250 (MQ=255)                                                                                                                      gaggaaCACCAGACTCCAGCTGTTTTTTGACCATTGATTCAATCGATTTTGGA‑TCGTCA‑ATGCCAACGTTAAAGTCACCGTCAGTGGC‑TAATAAAATG‑CGATTGATGCCGCCCTTAATAAACCCTTT‑C‑G‑T‑CGCCTGCTGAAAAGCCAGTTCCAGCACGGCACCGCCATTGGTACTGCCTTCGGCATCCAGCGAATCAATTGCGGCATTAATTTACGCCTTATGACTCCTGGAGAAAGAAGgcaatgcaat                                                                                                                          >  2:514175/5‑250 (MQ=255)                                                                                                                      gagTAACACGAGACTCCCGCTGTTTTTTCACCCTTGATTCAATCGATTTTCGA‑TCGTCA‑ATGCCAACGTTAATGTCACCGTCAGTGGA‑TAATAAAATG‑CGATTGATGCCGCCCTTAATAAACCCTTT‑C‑G‑T‑CGCCTGCTGATAAGCCAGTTCCAGCCCGGCACCGCCATTGGTCCTGCCTTCGGCATCCAGCGACTCAATTGCGGCATTAATTTCCGCCTTATGACTCCCGGAGATAGAAGgcaatgcaat                                                                                                                          >  1:368492/1‑250 (MQ=255)                                                                                                                       agTAACACCAGACTCCCGCTGTTTTTTGACCATTGATTCAATCGATTTTGGA‑TCGTCA‑ATGCCAACGTTAAAGTCACCGTCAGTGGC‑TAATAAAATG‑CGATTTATGCCGCCCTTAATAAACCCTTT‑C‑G‑T‑CGCCTGCTGATAAGCCAGTTCCAGCCCGGCACCGCCATTGGTACTGCCTTCGGCATC‑AGCGAATCAATTGCGGCATTAATTTCCGCCTTATGACTCCCGGAGATAGAAGGCAATGCAATACg                                                                                                                       <  1:567977/251‑1 (MQ=255)                                                                                                                            cccagaatacccGTTGTTTTTTGACTATGTATTCAATC‑ATTTTGGAGTC‑TCACATGCAACCGTTAAAGTCACTGTCTGTGGC‑TAATAAAATG‑CTATTGCGCCCTCCCTCAATAACCCCGTC‑C‑G‑C‑CGCCTGCTGATAAGCATTTTCCGCCCCGCCACCGCCATTGGTACTGCCTTCGGCATCCAGCGAATCAATTGCGGCATTAATTTTCGCCTTATGACTCCCGGAGATAGAAGGCAATGCAATACGTGAg                                                                                                                   <  2:617362/242‑1 (MQ=255)                                                                                                                                aGACGCCCGCTGTTTTTTGACCATTGATTCAATCGATTTTGGA‑TCATCA‑ATGCCAACGTTAAAGTCACCGTCAGTGGC‑TAATAAAATG‑CGATTGATGCCGCCCTTAATAAAACCTTT‑C‑G‑T‑CGCCTGCTGATAAGCCAGTTCCAGCCCGGCACCGCCATTGGTACTGCCTTCGGCATCCAGCTAATCAATTGAGGCATTACTTTCAGCATTATGACTCCCGGAGATAGAAAGAACAGCAATACggggttcac                                                                                                               >  2:776272/1‑245 (MQ=255)                                                                                                                                    gcccGTTGTTTTTGGCCCATTGATTCAATGGATGTTGGA‑GCGTCA‑GTGCTAACGTTAAAGTCTCCTTCAGGGGC‑TAATAAAATG‑CGTTTGAGACCGCCCTTAATAA‑CCCCTTGC‑G‑C‑CGCCGTCTGATAAGCCAGTTCCGGCCCTGCACCGCCATTGGTACTGCCTTCGGCATCCAGCGAATCAATTGCGGCATTAATTTCCGCCTTATGACTCCCGGAGATAGAAGGCAATGCAATACGGGAGTCGCCAGc                                                                                                           <  1:911344/250‑1 (MQ=255)                                                                                                                                            ttttttGACCATTGCTTCAATCGATTTTGGA‑TCGTCA‑ATGCCCACGTTAAAGTCACCGTCAGTGGC‑TAATAAAATG‑CGATTGATGCCGCCCTTAATAAACCCTTT‑C‑G‑T‑CGCCTGCTGATAAGCCAGTTCCAGCCCGGCACCGCCATTGGTACTGCCTTCGGCATCCAGCGAATCAATTTCGGCATTCATTTCCGCCTTATGACTCCCGGGGATAGATGGCAATGCAATACGGGGGTCGCCAGCGTCGGTc                                                                                                    >  2:102311/1‑250 (MQ=255)                                                                                                                                              ttttGACCATTGATTCAATCGATTTTGGC‑TCGTCA‑ATGCCAACGTTAAAGTCACCGTCAGTGGC‑TAATAAAATG‑CGATTGATGCCGCCCTTAATAAACCCTTT‑C‑G‑T‑CGCCTGCTGATAAGCCAGTTCCAGCCCGGCACCGCCATTGGTACTGCCTTCGGCATCCAGCGAATCAATTGCGGCATTAATTTCCGCCTTATGACTCCCGGAGATAGAAGGCAATGCAATACGGGAGTCGCCAGCGTAGGTCACg                                                                                                 >  2:592122/1‑251 (MQ=255)                                                                                                                                               tttGACCACTGATTCAATCGATTTTGGT‑TCGTCA‑ATGCCAACTTTAAAGTAACCGTCAGTGGT‑TAATAAA‑TGTCGATGGAGGCCGCCCTTAATAACCCTTTT‑C‑G‑T‑CGCCTGCTGATAAGCCGTTTCCAGCCCGGCACCGCCATTGGTACTGCCTTCGGCATCCAGCGAATCAATTGCGGCATTAATTTCCGCCTTATGCCTCCCGGAGATAGAAGGCAATGCAATACGGGAGTCGCCAGCGTAGGTCACg                                                                                                 <  2:698439/250‑1 (MQ=255)                                                                                                                                                 tGACCATTGATTCAATCGATTTTGGA‑TCGTCA‑ATGCCAACGTTAAAGTCACCGTCAGTGGC‑TAATAAAATG‑CGATTGATGCCGCCCTTAATAAACCCTTT‑C‑G‑T‑CGCCTGCTGATAAGCCAGTTCCAGCCCGGCACCGCCATTGGTACTGCCTTCGGCATCCAGCGAATCAATTGCGGCATTAATTTCCGCCTTATGACTCCCGGAGATAGAAGGCAATGCAATACGGGAGTCGCCAGCGTAGGTCACGATg                                                                                              >  1:83377/1‑251 (MQ=255)                                                                                                                                                       atttatcaaagtgttttgggaccGTCA‑ATGATAACTTTAAAGACCCCGGCAGTTGC‑AAATAAAAGG‑CGATGGATGCCGCCCTTAATACACCC‑TT‑C‑GGC‑CGCCGGCTAATAAGCCATTTCCAGCCCGGCACCGCCATTGGTACTGCCTTCGGCATCCAGCGAATCAATTGCGGCATTAATTTCCGCCTTATGACTCCCGGAGATAGAAGGCAATGCAATACGGGAGTCGCCAGCGTAGGTCACGATGGCAAt                                                                                         <  2:686559/229‑1 (MQ=255)                                                                                                                                                        tGATTCAATCGATTTTGGA‑TCGTCA‑ATGCCAACGTTAAAGTCACCGTCAGTGGC‑TAATAAAATG‑CGATTGATGCCGCCCTTAATAAACCCTTT‑C‑G‑T‑CGCCTGCTGATAAGCCAGTTCCAGCCCGGCACCGCCATTGGTACTGCCTTCGGCATCCAGCGAATCAATTGCGGCATTAATTTCCGCCTTATGACTCCCGGAGATAGAAGGCAATGCAATACGGGAGTCGCCAGCGTAGGTCACGATGGCAATGt                                                                                       >  1:636182/1‑251 (MQ=255)                                                                                                                                                            tCAATCGATTTTGGA‑TCGTCA‑ATGCCAACGTTAAAGTCCCCGTCATTGGC‑TAATAAAATG‑CGATTGATGCCTACCTTAATAACCCCTTT‑C‑G‑T‑CGCCTGCTGATAAGCCAGTTCCAGCCCGCCACCGCCATTGGTACTGCCTTCGGCATCCAGCGAATCAATTGCGGCATTAATTTCCGCCTTATGACTCCCGGAGATAGAAGGCAATGCAATACGGGAGTCGCCAGCGTAGGTCACGATGGCAATGTTAt                                                                                    <  1:444848/250‑1 (MQ=255)                                                                                                                                                             cAATCGTTTTTGGA‑TCGTCA‑ATGCCAACGTTAAAGTCCCCTTCAGTGGC‑TAATAAAATG‑CGATTGATGCCGCCCTAAAAAAACCCTTT‑C‑T‑T‑CCCCTGCTGATAAGCCATTTCCAGCCCGGCACCGCCATTGGTACTGCCTTCGGCATCCAGCGAATCAATTGCGGCATTAATTTCCGCCTTATGACTCCCGGAGATAGAAGGCAATGCAATACGGGAGTCGCCAGCGTAGGTCACGATGGCAATGTTATc                                                                                   <  1:688330/250‑1 (MQ=255)                                                                                                                                                                  gATTTTGGC‑ACGTCA‑TAGCCAATGTAAAAGCCCCCGTCAGTGGC‑TAATAAAATG‑CGATGGATGCCGCCCTTAATAAACCCTTG‑C‑G‑T‑CGCCTGCTGATAAGCAAGTTCCAGCCCGGCACCGCCATTGGTACTGCCTTCGGCATCCAGCGAATCAATTGCGGCATTAATTTCCGCCTTATGACTCCCGGAGATAGAAGGCAATGCAATACGGGAGTCGCCAGCGTAGGTCACGATGGCAATGTTATCCTGCTc                                                                             <  2:348093/251‑1 (MQ=255)                                                                                                                                                                   aTTTTGGA‑TCGTCA‑ATGCCAACGTTAAAGTCACCGTCAGTGGC‑TAATAAAATG‑CGATTGATGCCGCCCTTAATAAACCCTTT‑C‑G‑T‑CGCCTGCTGATAAGCCAGTTCCAGCCCGGCACCGCCATTGGTACTGCCTTCGGCATCCAGCGAATCAATTGCGGCATTAATTTCCGCCTTATGACTCCCGGAGATAGAAGGCAATGCAATACGGGAGTCGCCAGCGTAGGTCACGATGGCAATGTTATCCTGCTc                                                                             >  1:428261/1‑250 (MQ=255)                                                                                                                                                                    ttttGGA‑TCGTCA‑ATGCCAACGTTAAAGTCACCGTCAGTGGC‑TAATAAAATG‑CGATTGATGCCGCCCTTAATAAACCCTTT‑C‑G‑T‑CGCCTGCTGATAAGCCAGTTCCAGCCCGGCAACGCCATTGGTACTGCCTTCGGCATCCAGCGAATCAATTGCGGCATTAATTTCCGCCTTATGACTCCCGGAGATAGAAGGCAATGCAATACGGGAGTCGCCAGCGTAGGTCACGTTGGCAATGTTATCCTGCTCAc                                                                           >  1:621759/1‑251 (MQ=255)                                                                                                                                                                       tGGA‑TCGTCA‑ATGCCAACGTTAAAGTCACCGTCAGTGGC‑TAATAAAATG‑CGATTGATGCCGCCCTTAATAAACCCTTT‑C‑G‑T‑CGCCTGCTGATAAGCCAGTTCCAGCCCGGCACCGCCATTGGTACTGCCTTCGGCATCCAGCGAATCACTTGCGGCATTAATTTCCGCCTTATGACTCCCGGAGATAGAAGGCAATGCAATAAGGGAGTCGCCAGCGTAGGTCACGATGTCAATGTTATCCTGCTCACGa                                                                         >  2:346509/1‑250 (MQ=255)                                                                                                                                                                       gggA‑TCGTCA‑ATGCAAACGTTAAAGCCACCTTCAGTGGC‑AAATAAAATG‑CGATTGATCCCGCCCGTAATAAACCCTGT‑C‑G‑G‑CGCCTGCTGATAAGCCAGTTCCAGCCCGGCACCGCCATTGGTACTGCCTTCGGCATCCAGCGAATCAATTGCGGCATTAATTTCCGCCTTATGACTCCCGGAGATAGAAGGCAATGCAATACGGGAGTCGCCAGCGTAGGTCACGATGGCAATGTTATCCTGCTCACGaa                                                                        <  2:149929/250‑1 (MQ=255)                                                                                                                                                                          a‑TCGTCA‑ATGCCAACTTTAAAGTCACCGTCAGTGGC‑TAATAAAATG‑CGATTGAGGCCGCCCTTAATAAACCCTTT‑C‑G‑T‑CGCCTGCTGATAAGCCAGTTCCAGCCCGGCACCGCCATTGGTACTGCCTTCGGCATCCAGCGAATCAATTGCGGCATTAATTTCCGCCTTATGACTCCCGGAGATAGAAGGCAATGCAATACGGGAGTCGCCAGCGTAGGTCACGATGGCAATGTTATCCTGCTCACGAAGtt                                                                     <  1:104579/251‑1 (MQ=255)                                                                                                                                                                              gTCA‑ATGCCAACGTTAAAGTCACCGTCAGTGGC‑TAATAAAATG‑CGATTGATGCCGCCCTTAATAAACCCTTT‑C‑G‑T‑CGCCTGCTGATAAGCCAGTTCCAGCCCGGCACCGCCATTGGTACTGCCTTCGGCATCCAGCGAATCAATTGCGGCATTAATTTCCGCCTTATGACTCCCGGAGATAGAAGGCAATGCAATACGGGAGTCGCCAGCGTAGGTCACGATGGCAATGTTATCCTGCTCACGAAGTTCtt                                                                  >  1:42568/1‑251 (MQ=255)                                                                                                                                                                                aa‑aTGCCAACTTTAAAGTAACTGTCTGTGGA‑TAATAAAATG‑CGATTGTTGCCCCCCTTAAAAAACCTTTT‑C‑G‑T‑TGCCTGCTGATAAGCCAGTTCCAGCCCGGCACCGCCATTGGTTCTGCCTTCGGCATCCAGCGAATCAATTGCGGCATTAATTTCCGCCTTATGACTCCCGGAGATAGAAGGCAATGCAATACGGGAGTCGCCAGCGTAGGTCACGATGGCATTGTTATCCTGCTCACGAAGTTCTTTg                                                                <  1:143726/250‑1 (MQ=255)                                                                                                                                                                                            ttAAAGTCACCGTCAGTGGC‑TAATAAAATG‑CGATTGATACCGCCATTAATAAACCCTTT‑C‑G‑T‑CGCCTGCTGATAAGCCAGTTCCAGCCCGGCACCGCCATTGGTACTGCCTTCGGCATCCAGCGAATCAATTGCGGCATTAATTTCCGCCTTATGACTCCCGGAGATAGAAGGCAATGCAATACGGGAGTCGCCAGCGTAGGTCACGATGGCAATGTTATCCTGCTCACGAAGTTCTTTGACCAATAAttt                                                     >  1:873873/1‑251 (MQ=255)                                                                                                                                                                                                aGTCACCGTCAGTGGC‑TAATAAAATG‑CGATTGATGCCGCCCTTAATAAACCCTTT‑C‑G‑T‑CGCCTGCTGATAAGCCAGTTCCAGCCCGGCACCGCCATTGGTACTGCCTTCGGCATCCAGCGAATCAATTGCGGCATTAATTTCCGCCTTATGACTCCCGGAGATAGAAGGCAATGCAATACGGGAGTCGCCAGCGTAGGTCACGATGGCAATGTTATCCTGCTCACGAAGTTCTTTGACCAATAATTTCAAc                                                 >  1:75339/1‑251 (MQ=255)                                                                                                                                                                                                   cACCGTCAGTGGC‑TAATAAAATG‑CGATTGATGCCGCCCTTAATAAACCCTTT‑C‑G‑T‑CGCCTGCTGATAAGCCAGTTCCAGCCCGGCACCGCCATTGGTACTGCCTTCGGCATCCAGCGAATCAATTGCGGCATTAATTTCCGCCTTATGACTCCCGGAGATAGAAGGCAATGCAATACGGGAGTCGCCAGCGTAGGTCACGATGGCAATGTTATCCTGCTCACGAAGTTCTTTGACCAATAATTTCAACGa                                               <  1:844616/250‑1 (MQ=255)                                                                                                                                                                                                    cccGTCAGTGGC‑TAATAAAATG‑CGATTGATGCCGCCCTTAATAAACCCTTT‑C‑G‑T‑CGCCTGCTGATAAGCCAGTCCCAGCCCGGCACCGCCATTGGTACTGCCTTCGGCATCCAGCGAATCAATTGCGGCATTAATTTCCGCCTTATGACTCCCGGAGATAGAAGGCAATGCAATACGGGAGTCGCCAGCGTAGGTCACGATGGCAATGTTATCCTGCTCACGAAGTTCTTTGACCAATAATTTCAACGAAg                                             <  1:291884/250‑1 (MQ=255)                                                                                                                                                                                                      cGTCAGTGTC‑TAATAAAAAG‑CGATTGATGCCGCCCTTAATAACCCCTTT‑C‑G‑C‑CGCCTGCTGATAAGCCAGTTCCCGCCCGGCACCGCCATTGGTACTGCCTTCGGCATCCAGCGAATCAATTGCGGCATTAATTTCCGCCTTATGACTCCCGGAGATAGAAGGCAATGCAATACGGGAGTCGCCAGCGTAGGTCACGATGGCAATGTTATCCTGCTCACGAAGTTCTTTGACCAATAATTTCAACGAAGAc                                           <  1:786235/251‑1 (MQ=255)                                                                                                                                                                                                      cGTCAGTGGC‑TAATAAAATG‑CGATTGATGCCGCCCTTAATAAACCCTTT‑C‑G‑T‑CGCCTGCTGATAAGCCAGTTCCAGCCCGGCACCGCCATTGGTACTGCCTTCGGCATCCAGCGAATCAATTGCGGCATTAATTTCCGCCTTATGACTCCCGGAGATAGAAGGCAATGCAATACGGGAGTCGCCAGCGTAGGTCACGATGGCAATGTTATCCTGCTCACGAAGTTCTTTGACCAATAATTTCAACGAAGAc                                           >  1:812502/1‑251 (MQ=255)                                                                                                                                                                                                       gTCAGTGGC‑TAATAAAATG‑CGATTGAGGCCGCCCTTAA‑AAACCCCTTTC‑G‑T‑CGCCTGCTGATAAGCCAGTTCCAGCCCTGCACCGCCATTTGTACTGCCTTCGGCATCCAGCGAATCAATTGCGGCATTAATTTCCGCCTTATGACTCCCGGAGATAGAAGGCAATGCAATACGGGAGTCGCCAGCGTAGGTCACGATGGCAATGTTATCCTGCTCACGAAGTTCTTTGACCAATAATTTCAACGAAGACt                                          <  2:473073/251‑1 (MQ=255)                                                                                                                                                                                                        tCAGTGGC‑TAATAAAATG‑CGATTGATGCCGCCCTTAATAAACGCTTT‑C‑G‑T‑CGCCTGCTGATAAGCCAGTTCCAGCCCGGCACCGCCATTGGTACTGCCTTCGGCATCCAGCGAATCAATTGCGGCATTAATTTCCGCCTTATGACTCCCGGAGATAGAAGGCAATGCAATACGGGAGTCGCCAGCGTAGGTCACGTTGGCAATGTTATCCTGCTCACGAAGGTCTTTGCCCAATAATTTCAACGAAGACTg                                         >  1:769233/1‑251 (MQ=255)                                                                                                                                                                                                        ccAGTGGC‑TAAAAAAATG‑CGATTGATGCCGCCCTTAATAA‑CCCCTTGC‑G‑T‑CGCCTGCTGATAAGCCAGTTCCAGCCCGGCACCGCCATTGGTACTGCCTTCGGCATCCAGCGAATCAATTGCGGCATTAATTTCCGCCTTATGACTCCCGGAGATAGAAGGCAATGCAATACGGGAGTCGCCAGCGTAGGTCACGATGGCAATGTTATCCTGCTCACGAAGTTCTTTGACCAATAATTTCAACGAAGACTg                                         <  2:83377/250‑1 (MQ=255)                                                                                                                                                                                                         cAGTGTC‑TAATAAAAGG‑CGATTGATCCCCCCCTTAATAACCCCTTT‑C‑G‑C‑CGCCTGCGGATAAGCCATTTCCAGCCCGGCACCGCCATTGGTACTGCCTTCGGCATCCAGCGAATCAATTGCGGCATTAATTTCCGCCTTATGACTCCCGGAGATAGAAGGCAATGCAATACGGGAGTCGCCAGCGTAGGTCACGATGGCAATGTTATCCTGCTCACGAAGTTCTTTGACCAGTAATTTCAACGAAGACTg                                         <  1:906484/250‑1 (MQ=255)                                                                                                                                                                                                          aGTGGC‑TAATAAAATG‑CGATTGATGCCGCCCTTAATAAACCCTTT‑C‑G‑T‑CGCCTGCTGATAAGCCAGTTCCAGCCCGGCACCGCCATTGGTACTGCCTTCGGCATCCAGCGAATCAATTGCGGCATTAATTTCCGCCTTATGACTCCCGGAGATAGAAGGCAATGCAATACGGGAGTCGCCAGCGTAGGTCACGATGGCAATGTTATCCTGCTCACGAAGTTCTTTGACCAATAATTTCAACGAAGACTgg                                        >  1:246309/1‑250 (MQ=255)                                                                                                                                                                                                              gC‑TAATAAAATG‑CGATTGATGCCGCCCTTAATAAACCCTTT‑C‑G‑T‑CGCCTGCTGATAAGCCAGTTCCAGCCCGGCACCGCCATTGGTACTGCCTTCGGCATCCAGCGAATCAATTGCGGCATTAATTTCCGCCTTATGACTCCCGGAGATAGAAGGCAATGCAATACGGGAGTCGCCAGCGTAGGTCACGATGGCAATGTTATCCTGCTCACGAAGTTCTTTGCCCAATACTTTCAACGAAGACTGGATa                                     >  1:920540/1‑249 (MQ=255)                                                                                                                                                                                                                   ataaAATG‑CGATTGATGCCGCCCTTAATAAACCCTTT‑C‑G‑T‑CGCCTGCTGATAAGCCAGTTCCAGCCCGGCACCGCCATTGGTACTGCCTTCGGCATCCAGCGAATCAATTGCGGCATTAATTTCCGCCTTATGACTCCCGGAGATAGAAGGCAATGCAATACGGGAGTCGCCAGCGTAGGTCACGATGGCAATGTTATCCTGCTCACGAAGTTCTTTGACCAATAATTTCAACGAAGACTGGATAAGTGGc                               >  1:656579/1‑251 (MQ=255)                                                                                                                                                                                                                       aacg‑CGATTGAGGCCGCCCTTAATAAACCCTTT‑C‑G‑T‑CGCCTGCTGATAAGCCATTTCCAGCCCGCCTCCGCCATTGGTACTGCCTTCGGCATCCAGCGAATCAATTGCGGCATTAATTTCCGCCGTATGACTCCCGGAGATAGAAGGCAATGCAATACGGGAGTCGCCAGCGTAGGTCACGATGGCAATGTTATCCTGCTCACGAAGTTCTTTGACCAATAATTTCAACGAAGACTGGATAAGTGGCAAAc                           <  1:608741/248‑1 (MQ=255)                                                                                                                                                                                                                       aaTG‑CGATTGATGCCGCCCTTAATAAACCCTTT‑C‑G‑T‑CGCCTGCTGATAAGCCAGTTCCAGCCCGGCACCGCCATTGGTACTGCCTTCGGCATCCAGCGAATCAATTGCGGCATTAATTTACGCCTTATGACTACCGGAGATAGAAGGCAATGCACTACGGGAGTCGCCAGCGTAGGTCACGATGCCAATGTTATCCTGCTCACGAAGTTCTTTGACCAATAATTTCAACGAAGACTGGATAAGTGGCAAAc                           >  2:987244/1‑251 (MQ=255)                                                                                                                                                                                                                          g‑CGATTGATGCCGCCCTTAATAAACCCTTT‑C‑G‑T‑CGCCTGCTGATAAGCCAGTTCCAGCCCGGCACCGCCATTGGTACTGCCTTCGGCATCCAGCGAATCAATTGCGGCATTAATTTCCGCCTTATGACTCCCGGAGATAGAAGGCAATGCAATACGGGAGTCGCCAGCGTAGGTCACGATGGCAATGTTATCCTGCTCACGAAGTTCTTTGACCAATAATTTCAACGAAGACTGGATAAGTGGCAAACGtt                        >  1:288645/1‑251 (MQ=255)                                                                                                                                                                                                                            cGATTGCTGCCGCCCTTAATAAACCCTTT‑C‑G‑T‑CGCCTGCTGATAAGCCAGTTCCAGCCCGGCACCGCCATTGGTACTGCCTTCGGCATCCAGCGAATCAATTGCGGCATTAATTTCCGCCTTATGACTCCCGGCGATAGAAGGCAATGCAATACGGGAGTCGCCAGCGTAGGTCACGATGGCAATGTTATCCTGATCACGAAGTTCTTTGACCAATAATTTCAACGAAGACTGGATAAGTGGCAAACGTtc                       >  2:657667/1‑251 (MQ=255)                                                                                                                                                                                                                             gATTGATGCCGCCCTTAATAAACCCTTT‑C‑G‑T‑CGCCTGCTGATAAGCCAGTTCCAGCCCGGCACCGCCATTGGTACTGCCTTCGGCATCCAGCGAATCAATTGCGGCATTAATTTCCGCCTTATGACTCCCGGAGATAGAAGGCAATGCAATACGGGAGTCGCCAGCGTAGGTCACGATGGCAATGTTATCCTGCTCACGAAGTTCTTTGACCAATAATTTCAACGAAGACTGGATAAGTGGCAAACGTtc                       >  1:756793/1‑250 (MQ=255)                                                                                                                                                                                                                                 gATGCCGCCCTTAATAA‑CCCCTT‑CCG‑T‑CGCCTGCTGATAAGCCAGTTCCAGCCCGGCACCGCCATTGGTACTGCCTTCGGCATCCAGCGAATCAATTGCGGCATTAATTTCCGCCTTATGACTCCCGGAGATAGAAGGCAATGCAATACGGGAGTCGCCAGCGTAGGTCACGATGGCAATGTTATCCTGCTCACGAAGTTCTTTGACCAATAATTTCAACGAAGACTGGATAAGTGGCAAACGTTCATCAg                  <  1:328348/251‑1 (MQ=255)                                                                                                                                                                                                                                  aTGCCGCCCTTAATAAACCCTTT‑A‑G‑T‑CGCCTGCTGATAAGCCAGTTCCAGCCCGGCACCGCCATTGGTACTGCCTTCGGCATCCAGCGAATCAATTGCGGCATTAATTTCCGCCTTATGACTCCCGGAGATAGAAGGCAATGCAATACGGGAGTCGCCAGCGTAGGTCACGATGGCAATGTTATCCTGCTCACGAAGTTCTTTGACCAATAATTTCAACGAAGACTGGATAAGAGGCAAACGTtcatc                    >  1:664331/1‑248 (MQ=255)                                                                                                                                                                                                                                     ccgccCTTAATAAACCCTTT‑C‑G‑T‑CGCCTGCTGATAAGCCAGTTCCAGCCCGGCACCGCCATTGGTACTGCCTTCGGCATCCAGCGAATCAATTGCGGCATTAATTTCCGCCTTATGACTCCCGGAGATAGAAGGCAATGCAATACGGGAGTCGCCAGCGTAGGTCACGATGGCAATGTTATCCTGCTCACGAAGTTCTTTGACCAATAATTTCAACGAAGACTGGATAAGTGGCAAACGTTCATCAGAAAt              >  1:805514/1‑251 (MQ=255)                                                                                                                                                                                                                                      cgccCTTAATAAACCCTTT‑C‑T‑C‑CGCCTGCTGATAAGCCAGTTCCAGCCCGGCACCGCCATTGGTACTGCCTTCGGCATCCAGCGAATCAATTGCGGCATTAATTTCCGCCTTATGACTCCCGGAGATAGAAGGCAATGCAATACGGGAGTCGCCAGCGTAGGTCACGATGGCAATGTTATCCTGCTCACGAAGTTCTTTGACCAATAATTTCAACGAAGACTGGATAAGTGGCAAACGTTCATCAGAAATc             <  1:804898/251‑1 (MQ=255)                                                                                                                                                                                                                                         ccTTAATAAACCCTTT‑C‑G‑T‑CGCCTGCTGATAAGCCAGTTCCAGCCCGGCACCGCCATTGGTACTGCCTTCGGCATCCAGCGAATCAATTGCGGCATTAATTTCCGCCTTATGACTCCCGGAGATAGAAGGCAATGCAATACGGGAGTCGCCAGCGTAGGTCACGATGGCAATGTTATCCTGCTCACGAAGTTCTTTGACCAATAATTTCAACGAAGACTGGATAAGTGGCAAACGTTCATCAGAAATCAtt          >  1:989305/1‑251 (MQ=255)                                                                                                                                                                                                                                         ccTTAATAAACCCTTT‑C‑G‑T‑CGCCTGCTGATAAGCCAGTTCCAGCCCGGCACCGCCATTGGTACTGCCTTCGGCATCCAGCGAATCAATTGCGGCATTAATTTCCGCCTTATGACTCCCGGAGATAGAAGGCAATGCAATACGGGAGTCGCCAGCGTAGGTCACGATGGCAATGTTATCCTGCTCACGAAGTTCTTTGACCAATAATTTCAACGAAGACTGGATAAGTGGCAAACGTTCATCAGAAATCAtt          >  1:873161/1‑251 (MQ=255)                                                                                                                                                                                                                                            taataaACCCTTT‑C‑G‑T‑CGCCTGCTGATAAGCCAGTTCCAGCCCGGCACCGCCATTGGTACTGCCTTCGGCATCCAGCGAATCAATTGCGGCATTAATTTCCGCCTTATGACTCCCGGAGATAGAAGGCAATGCAATACGGGAGTCGCCAGCGTAGGTCACGATGGCAATGTTATCCTGCTCACGAAGTTCTTTGACCAATAATTTCAACGAAGACTGGATAAGTGGCAAACGTTCATCAGAAATCATTg         <  1:208921/249‑1 (MQ=255)                                                                                                                                                                                                                                                  aCCCTTT‑C‑G‑T‑CGCCTGCTGATAAGCCAGTTCCAGCCCGGCACCGCCATTGGTACTGCCTTCGGCATCCAGCGAATCAATTGCGGCATTAATTTCCGCCTTATGACTCCCGGAGATAGAAGGCAATGCAATACGGGAGTCGCCAGCGTAGGTCACGATGGCAATGTTATCCTGCTCACGAAGTTCTTTGACCAATAATTTCAACGAAGACTGGATAAGTGGCAAACGTTCATCAGAAATCATTGAACCAGa  >  1:47415/1‑250 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                          ATACTTTCTGCGCTTCAGAGAGGGTATCAATGTAGCTGTAGTTGCCGTTACCAACATCGGCAATTCGCACCATCATTGCCTCGTTGTAATTGCTATTCCCCACGCCAAACGTCGACAGAGTAACACCAGACTCCCGCTGTTTTTTGACCATTGATTCAATCGATTTTGGA‑TCGTCA‑ATGCCAACGTTAAAGTCACCGTCAGTGGC‑TAATAAAATG‑CGATTGATGCCGCCCTTAATAAACCCCTT‑C‑G‑T‑CGCCTGCTGATAAGCCAGTTCCAGCCCGGCACCGCCATTGGTACTGCCTTCGGCATCCAGCGAATCAATTGCGGCATTAATTTCCGCCTTATGACTCCCGGAGATAGAAGGCAATGCAATACGGGAGTCGCCAGCGTAGGTCACGATGGCAATGTTATCCTGCTCACGAAGTTCTTTGACCAATAATTTCAACGAAGACTGGATAAGTGGCAAACGTTCATCAGAAATCATTGAACCAGA  >  NC_000913/2384567‑2385053

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑