Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,987,499	C→A	intergenic (+71/+8)	ftnB → / ← yecJ	putative ferritin‑like protein/DUF2766 domain‑containing protein YecJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,987,499	0	C	A	100.0%	181.9 / NA	63	intergenic (+71/+8)	ftnB/yecJ	putative ferritin‑like protein/DUF2766 domain‑containing protein YecJ
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (35/28);  total (35/28)

AACTGAATGATGATTCAACCGTCAATTTCCTGCGCGATCTGGAAAAAGAACAGCAGCATGATGGTCTGTTGCTGCAAACCATTCTTGATGAAGTGCGCAGTGCGAAACTTGCGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCAATAAGCGCGATGGCTTTTTGGATTGCCCGCATGGTGACCGGGTCTGCGGCGGCGGGATGGTTAGTAAAGTCGATATTCTTCAGCTGACTGGACATTTTTTCACGAACTTCAACGGGCGCGATTACATCGAGAACATCCAGAATTTGTTTGATAACCAACTGGCAAGCAACCACATCAGAAACCAATTCCTGATCGGCATT  >  NC_000913/1987253‑1987743                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                     aaCTGAATGATGATTCAACCGTCAATTTCCTGCGCGATCTGGAAAAAGAACAGCAGCATGATGGTCTGTTGCTGCAAACCATTCTTGATGAAGTGCGCAGTGCGAAACTTGCGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGAGGtt                                                                                                                                                                                                                                                  >  1:477885/1‑251 (MQ=255)     gAATGATGATTCAATCGTCAATTTCCTGCGCGATCTGGAAAAAGAACAGCAGCATGATGGGCTGTTGCTGCAAACCATTCTTGATGAAGTGCGCAGTGCGAAACTTGCGGGTATGTGCCCTGTGCAGACCGACCAACATTTTCTGAATGTCGTGTCACACCAGCTGCAGTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGAGGtttt                                                                                                                                                                                                                                                <  1:680947/249‑1 (MQ=255)             atTCAACCGTCAATTTCCTGCGCGATCTGGAAAAAGAACAGCAGCATGATGGTCTGTTGCTGCAAACCATTCTTGATGAAGTGCGCAGTGCGAAACTTGCGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGAGGTTTTTTTAATGGGa                                                                                                                                                                                                                                      <  1:701876/251‑1 (MQ=255)               tCAACCGTCAATTTCCTGCGCGATCTGGAAAAAGAACAGCAGCATGATGGTCTGTTGCTGCAAACCATTCTTGATGAAGTGCGCAGTGCGAAACTTGCGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGAGGTTTTTTTAATGGGAt                                                                                                                                                                                                                                     <  1:929794/250‑1 (MQ=255)               tCAACCGTCAATTTCCTGCGCGATCTGGAAAAAGAACAGCAGCATGATGGTCTGTTGCTGCAAACCATTCTTGATGAAGTGCGCAGTGCGAAACTTGCGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCAGCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGAGGTTTTTTTAATGGGAt                                                                                                                                                                                                                                     <  1:112763/250‑1 (MQ=255)                cAACCGTCAATTTCCTGCGCGATCTGGAAAAAGAACAGCAGCATGATGGTCTTTTGCTGCAAACTATTCGTGATGAAGTGCGCAGTGCGAATCTTGCGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATGGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGAGGTTTTTTTAATGGGATTc                                                                                                                                                                                                                                   <  2:36254/251‑1 (MQ=255)                       cAATTTCCTGCGAGATCTGGAAAAAGAACAGCAGCATGATGGTCTGTTGCTGCAAACCATTCTTGATGAAGTGCGCAGTGCGAAACTTGCGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGAGGTTTTTTTAATGGGATTCACCCtgt                                                                                                                                                                                                                            >  1:853685/1‑251 (MQ=255)                                gcgcGATCTGGAAAAAGAACAGCAGCATGATGGTCTGTTGATGCAAACCATTCTTGATGAAGTGCGCAGTGCGAAACTTGCGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGAGGTTTTTTTAATGGGATTCACCCTGTGGGGTaaa                                                                                                                                                                                                                    >  2:97779/1‑250 (MQ=255)                                        tGGAAAAAGAACAGCAGCATGATGGTCTGTTGCTGCAAACCATTCTTGATGAAGTGCGCAGTGCGAAACTTGCGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGAGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTc                                                                                                                                                                                                           >  1:811415/1‑251 (MQ=255)                                         ggAAAAAGAACAGCAGCATGATGGTCTGTTGATGCAAACCATTCTTGATGAAGTGCGCAGTGCGAAACTTGCGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGAGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCa                                                                                                                                                                                                          <  1:2763/251‑1 (MQ=255)                                             aaaGAACAGCAGCATGATGGTCTGTTGCTGCAAACCATTCTTGATGAAGTGCGCAGTGCGAAACTTGCGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGAGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCAATa                                                                                                                                                                                                       >  2:730901/1‑250 (MQ=255)                                              aaGAACAGCAGCATTATGGTCTGATGCTGCAATCCATGCGTGAGGAAGGGCGCAGTGCGAAACTTGCGGGTATGTGCCCCGTGCAGACCGACCAACATGTTCTGAATGGCGTGTCACACCAGTTGCATTGATCATCATCTGCGCTAATGCATTGCGCCGATGAAGGGTTTGAGAAACCGCTGCCTCATCTGTGTGAAGCAGAGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCAATAAg                                                                                                                                                                                                     <  1:82197/251‑1 (MQ=255)                                              aaGAACAGCAGCATGATGGTCTGTTGCTGCAAACCATTCTTGATGAAGTGCGCAGTGCGAAACTTGCGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGAGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCAATa                                                                                                                                                                                                       >  2:26562/1‑249 (MQ=255)                                                gAACAGCAGCATGATGGTCTGTTGCTGCAAACCATTCTTGATGAAGTGCGCAGTGCGAAACTTGCGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGAGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCAATAAgcg                                                                                                                                                                                                   >  1:170987/1‑251 (MQ=255)                                                    agcagcATGATGGTCTGTTGCTGCAACCCATTCTTGATGAAGTGCGCAGTGCGAAACTTGCGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCAGCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGAGGTGTTTTTAATGTGATTCACCCTGTGGGGTAAACTTGAGTGCAATAAGCGCGAt                                                                                                                                                                                               <  2:883509/251‑1 (MQ=255)                                                       agcaTGATGGTCTGTTGCTGCAAACCATTCTTGATGAAGTGCGCAGTGCGAAACTTGCGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGAGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCAATAAGCGCGATGGc                                                                                                                                                                                            <  2:658346/251‑1 (MQ=255)                                                        gcaTGATGGTCTGTTGCTGCAAACCATTCTTGATGAAGTGCGCAGTGCGAAACTTGCGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGAGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCAATAAGCGCGATGGCt                                                                                                                                                                                           <  1:634236/251‑1 (MQ=255)                                                         caTGATGGTCTGTTGCTGCAAACCATTCTTGATGAAGTGCGCAGTGCGAAACTTGCGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGAGGTTTTTTTAATGGGATTCACCCTGTGGGGTACACTTGAGTTCAATAAGCGCGATGGCtt                                                                                                                                                                                          >  2:300933/1‑251 (MQ=255)                                                          atgatgGTCTGTTGCTGCAAACCATTCTTGATGAAGTGCGCAGTGCGAAACTTGCGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATCGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGAGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCAATAAGCGCGATGGCttt                                                                                                                                                                                         >  1:434694/1‑251 (MQ=255)                                                              tgGTCTGTTGCTGCAAACCATTCTTGATGAAGTGCGCAGTGCGAAACTTGCGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGCAGCAGAGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCAATAAGCGCGATGGCTTTTTgg                                                                                                                                                                                     >  2:852293/1‑251 (MQ=255)                                                                 tCTGTTGCTGCAAACCATTCTTGATGAAGTGCGCAGTGCGAAACTTGCGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGAGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCAATAAGCGCGATGGCTTTTTGGAtt                                                                                                                                                                                  >  2:14724/1‑251 (MQ=255)                                                                          gcAAACCATTCTTGATGAAGTGCGCAGTGCGAAACTTGCGGGAATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGAGGTTTTTTTAATGGGATTCACCCTGTGGGGAAAACTTGAGTTCAATACGCGCGTTGGCTTTTTGGATTGCCCGCATg                                                                                                                                                                         >  1:784305/1‑251 (MQ=255)                                                                              aCCATTCTTGATGAAGTGCGCAGTGCGACACTTGCGGGTATGTGCTCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATAGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGAGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCAATAATCGCGATGTCTTTTTGGATTgacagcctggat                                                                                                                                                                      >  2:166529/1‑239 (MQ=255)                                                                                cATTCTTGATGAAGTGCGCAGTGCGAAACTTGCGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGAGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCAATAAGCGCGATGGCTTTTTGGATTGCCCGCATGGTGAcc                                                                                                                                                                   >  1:195886/1‑251 (MQ=255)                                                                                       gatgaAGTGCGCAGTGCGAAACTTGCGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGAGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCAATAAGCGCGATGGCTTTTTGGATTGCCCGCATGGTGACCGGGTCTg                                                                                                                                                            <  1:161083/251‑1 (MQ=255)                                                                                                    gTGCTAAACTTGCGGGTATGTGCTCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGAGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCAATAAGCGCGATGGCTTTTTGGATTGCCCGCATGGTGACCGGGTCTGCGGCGGCGGGATg                                                                                                                                               <  2:145822/251‑1 (MQ=255)                                                                                                         aaactaacGGGTATGTAACCTGTGCAGAACGACCAACATGTTCTGAATGTAGAGTCACACCAGCTGCATTGCTCATCATCGGCGCTAATGCATTGCGCCGCTGAAGGTTATGCGAAACCGCTGCATCATCTGTTTGAAGCAGAGGTTTTTTTAATGGGATTCACACTGTGGGGAAAAGTTGAGTTCAATAAGCGCGATGGCTTTTTGGATTGACCGCATGGTGACCGGGTCTGCGGCGGCGGGGTGGttgg                                                                                                                                          >  2:443365/8‑249 (MQ=255)                                                                                                          aaCTTTCGGGTATGTGAACTGTGCAATCAGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCCTTGCGTCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGAGGTTTTTTTACTGGGATTCCCCCTGTTGGGTAACCTTGTGTTCAATAAGAGCGATTGCTTTTTGTATTCCCCGCATGGTGCCCGGTTCTGCTGCAGCGGGCTGGTTatt                                                                                                                                         >  2:921497/1‑249 (MQ=255)                                                                                                          aaCTTGCGGGTATGTCCCCTGTGCAGACCGCCCAACACGTTCTGAATGTCGTGTCAGACCAGCTGCATTGATCATCATCGGAGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGAGGTTTTTTTAATGGGATTCACCCTGTGGGGTACACTTGAGTTCAATAAGCGCGATGGCTTTTTGGATTGCCCGAATGGCGACAGGGTCTGTGGAGGCGGGATgggt                                                                                                                                            >  2:12138/1‑246 (MQ=255)                                                                                                          aaCTTGAGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGACGCAGAGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTAAGTTCAATAAGCGCGCTGGCTTTTTGGATTGCCCGCATGGTGACCGGGTCTACGGCGGCGGGATGGTTAGt                                                                                                                                         >  2:921507/1‑251 (MQ=255)                                                                                                           aCTTGCGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGAGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCAATAAGCGCGATGGCTTTTTGGATTGCCCGCATGGTGACCGGGTCTGCGGCGGCGGGATGGTTAgg                                                                                                                                         >  1:645499/1‑249 (MQ=255)                                                                                                               gCGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGAGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCAATAAGCGCGATGGCTTTTTGGATTGCCCGCATGGGGACCGGGTCTGCGGCGGCGGGATGGTTAGTAAAGt                                                                                                                                    <  2:421094/251‑1 (MQ=255)                                                                                                                   gTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGAGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCAATAAGCGCGATGGCTTTTTGGATTGCCCGCATgg                                                                                                                                                                        <  1:197403/211‑1 (MQ=255)                                                                                                                   gTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGAGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCAATAAGCGCGATGGCTTTTTGGATTGCCCGCATgg                                                                                                                                                                        >  2:197403/1‑211 (MQ=255)                                                                                                                          cccTGTTCAGACCGACCAACATGTTCTGAATTTCGTTTCCCCCCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTGTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGAGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCAATAAGCGCGATGGCTTTTTGGATTGCCCGCATGGTGACCGGGTCTGCGGCGGCGGGATGGTTAGTAAAGTCGATAttcttc                                                                                                                         <  2:455452/251‑1 (MQ=255)                                                                                                                              gtgCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGAGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCAATAAGCGCGATGGCTTTTTGGATTGCCCGCATGGTGACCGGGTCTGCGGCGGCGGGATGGTTAGTAAAGTCGATATTCTTCAGCt                                                                                                                     >  1:655131/1‑251 (MQ=255)                                                                                                                                   gaccgaccACCATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGAGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCAATAAGCGCGATGGCTTTTTGGATTGCCCGCATGGTGACCGGGTCTGCGGCGGCGGGGTGGTTAGTAAAGTCGATATTCTTCAgctggctg                                                                                                                >  2:647376/1‑251 (MQ=255)                                                                                                                                    accgaccAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGAGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCAATAAGCGCGATGGCTTTTTGGATTGCCCGCATGGTGACCGGGTCTGCGGCGGCGGGATGGTTAGTAAAGTCGATATTCTTCAGCTGACTgg                                                                                                               <  1:647376/251‑1 (MQ=255)                                                                                                                                    accgaccAACATGTTCTGAATGTCGTGGCACACCAGCTTCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCGCATCTGTTTGAAGCAGAGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCAATAAGCGCGATGGCTTTTTGGATTGCCCGCATGGTGACCGGGTCTGCGGCGGCGGGATGGTTAGTAAATTCGATATTCTTCAGCTGACTgg                                                                                                               <  2:768675/251‑1 (MQ=255)                                                                                                                                      cgaccAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGAGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCAATAAGCGCGATGGCTTTTTGGATTGCCCGCATGGTGACCGGGTCTGCGGCGGCGGGATGGTTAGTAAAGTCGATATTCTTCAGCTGACTGGAc                                                                                                             <  1:735162/251‑1 (MQ=255)                                                                                                                                             cATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGAGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCACTAAGCGCGATGGCTTTTTGGATTGCCCGCATGGTGACCGGGTCTGCGGCGGCGGGATGGTTAGTAAAGTCGATATTCTTCAGCTGACTGGACAtttttt                                                                                                      >  1:45447/1‑251 (MQ=255)                                                                                                                                                gTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGAGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGCGTTCAATAAGCGCGATGGCTTTTTGGATTGCCCGCATGGTGACCGGGTCTGCGGCGGCGGGATGGTTAGGAAAGTCGATATTCTTCAGCTGCCTGGACATTTTTTCAc                                                                                                   >  2:727404/1‑251 (MQ=255)                                                                                                                                                         gTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGAGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTAAATAAGCGCGATGGCTTTTTGGATTGCCCGCATGGTGACCGGGTCTGCGGCAGCGGGATGGTTAGTAAAGTCGATATTCTTCAGCTGACTGGACATTTTTTCCCGAACTTCa                                                                                           >  1:955984/1‑250 (MQ=255)                                                                                                                                                             tgtCACACCCACTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGCTGACGGTTTTGAGACACCGCTGCCTCCTCTGTTTGAAGCAGAGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCAATAAGCGCGATGGCTTTTTGGATTGCCCGCATGGTGACCGGGTCTGCGGCGGCGGGATGGTTAGTCAAGTCGATATTCTTCAGCTGACTGGACATTTTTTCaagacctacaccggg                                                                                      >  2:961802/1‑237 (MQ=255)                                                                                                                                                                     cAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGAGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCAATAAGCGCGATGGCTTTTTGGATTGCCCGCATGGTGACCGGGTCTGCGGCGGCGGGATGGTTAGTAAAGTCGATATTCTTCAGCTGACTGGACATTTTTTCACGAACTTCAACGGGCGCGAtt                                                                               >  1:163607/1‑250 (MQ=255)                                                                                                                                                                        cTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGAGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCAATAAGCGCGATGGCTTTTTGGATTGCCCGCATGGTGACCGGGTCTGCGGCGGCGGGATGGTTAGTAAAGTCGATATTCTTCAGCTGACTGGACATTTTTTCACGAACTTCAACGGGCGCGATTACAt                                                                           >  1:648457/1‑251 (MQ=255)                                                                                                                                                                         tGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGAGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCAATAAGCGCGATGGCTTTTTGGATTGCCCGCATGGTGACCGGGTCTGCGGCGGCGGGGTGGTTAGTAAAGGCGATATTCTTCAGCTGGCTGGACATTTTTTCACGAACTTCAACGGGCGCGATTACATc                                                                          >  2:195465/1‑251 (MQ=255)                                                                                                                                                                          gCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGAGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCAATAAGCGCGATGGCTTTTTGGATTGCCCGCATGGTGACCGGGTCTGCGGCGGCGGGATGGTTAGTAAAGTCGATATTCTTCAGCTGACTGGACATTTTTTCACGAACTTCAACGGGCGCGATTACATCg                                                                         <  1:14724/251‑1 (MQ=255)                                                                                                                                                                           cATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGAGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCAATAAGCGCGATGGCTTTTTGGATTGCCCGCATGGTGACCGGGTCTGCGGCGGCGGGATGGTTAGTAAAGTCGATATTCTTCAGCTGACTGGACATTTTTTCACGAACTTCAACGGGCGCGAGTACATCg                                                                         >  1:94562/1‑250 (MQ=255)                                                                                                                                                                               gATCATCATCGGCGCTAATGCAGTGCGACTATGAATTTTTTGAAAAACCGCTGCCTCATCTGTGTGAAGCATAGGTTTTTTTAATGGTATTCACCCTGTGGGGTAAACTTGAGTTCAATAAGCGCGATGTCTTTTTGGATTGCCCGCATGGTGACCGGGTCTGCTGCGGCGGGATGGTTAGTAAAGTCGATATTCTTCAGCTGACTGGACATTTTTTCACGAACTTCAACGGGCGCGATTACATCGAGaa                                                                     <  2:687994/250‑1 (MQ=255)                                                                                                                                                                                          gcgcTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGAGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCAATAAGCGCGATGGCTTTTTGGATTGCCCGCATGGTGACCGGGTCTGCGGCGGCGGGATGGTTAGTAAAGTCGATATTCTTCAGCTGACTGGACATTTTTTCACGAACTTCAACGGGCGCGATTACATCGAGAACATCCAGAAttt                                                         <  1:119902/251‑1 (MQ=255)                                                                                                                                                                                               gaTGCATGGCGCCGAGGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGAGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCAATAAGCGCGATGGCTTGTTGGATTGCCCGCATGGTGACCGGGTCTGCGGCGGCGGGATGGTTAGTAAAGTCGATATTCTTCAGCTGACTGGACATTTTTTCACGAACTTCAACGGGCGCGATTACATCGAGAACATCCAGAAtttgttt                                                     <  2:477885/249‑1 (MQ=255)                                                                                                                                                                                                aTGCATTGCGCCGATGAAGGTTTTGAGCAAACGCTGCCTCATCTGTTTGAAGCAGAGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCAATAAGCGCGATGGCTTTTTGGATTGCACGCATGGTGACCGGGTCTGCGGCGGCGGGATGGTTAGTAAAGTCGATATTCTTCAGCTGACTGGACATTTTTTCACGACCTTCAACGGGCGCGATTACATCGAGAACCTTCAGAAtttgtttg                                                    >  2:136271/1‑250 (MQ=255)                                                                                                                                                                                                aTGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGAGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCAATAAGCGCGATGGCTTTTTGGATTGCCCGCATGGTGACCGGGTCTGCGACGGCGGGATGGTTAGTAAAGTCGATATTCTTCACCTGCCGGGACATTTTTTCACGAACTTCACCGGGCGCGATTACATCGCGAACATCCAGAAtttgtttgt                                                   >  2:94820/1‑250 (MQ=255)                                                                                                                                                                                                   cATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGAGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCAATAAGCGCGATGGCTTTTTGGATTGCCCGCATGGTGACCGGGTCTGCGGCGGCGGGATGGTTAGTAAAGTCGATATTCTTCAGCTGACTGGACATTTTTTCACGAACTTCAACGGGCGCGATTACATCGAGAACATCCAGAATTTGTTTGATa                                                 >  1:287764/1‑250 (MQ=255)                                                                                                                                                                                                        cgcCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGAGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCAATAAGCGCGATGGCTTTTTGGATTGCCCGCATGGTGACCGGGTCTGCGGCGGCGGGATGGTTAGTAAAGTCGATATTCTTCAGCTGACTGGACATTTTTTCACGAACTTCAACGGGCGCGATTACATCGAGAACATCCAGAATTTGTTTGATAACCAAc                                           <  1:613257/251‑1 (MQ=255)                                                                                                                                                                                                          ccGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGAGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCAATAAGCGCGATGGCTTTTTGGATTGCCCGCATGGTGACCGGGTCTGCGGCGGCGGGATGGTTAGTAAAGTCGATATTCTTCAGCTGACTGGACATTTTTTCACGAACTTCAACGGGCGCGATTACATCGAGAACATCCAGAATTTGTTTGATAACCAACTg                                         <  2:65839/251‑1 (MQ=255)                                                                                                                                                                                                                  ggTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGAGGTTTTTTTAATTGGATTCACCCTGGGGGGTAAACTTGAGTTCAATAAGCGCGATGGCTTTTTGGATTGCCCGCATGGTGACCGGGTCTGCGGCGGCGGGATGGTTAGTAAAGTCGATATTCTTCAGCTGACTGGACATTTTTTCACGAACTTCAACGGGCGCGATTACATCGAGAACATCCAGAATTTGGTTGATAACCAACTGgcaagcaa                                 <  1:751732/251‑1 (MQ=255)                                                                                                                                                                                                                     tttGAGAAACCGCTGCCTCATCTGTTTGAAGCAGAGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCAATAAGCGCGATGGCTTTTTGGATTGCCCGCATGGTGACCGGGTCTGCGGCGGCGGGATGGTTAGTAAAGTCGATATTCTTCAGCTGACTGGACATTTTTTCACGAACTTCAACGGGCGCGATTACATCGAGAACATCCAGAATTTGTTTGATAACCAACTGGCAAGCAAcc                               <  1:300933/250‑1 (MQ=255)                                                                                                                                                                                                                          gaAACCGCTGCCTCATCTGTTTGAAGCAGAGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCAATAAGCGCGATGGCTTTTTGGATTGCCCGCATGGTGACCGGGTCTGCGGCGGCGGGATGGTTAGTAAAGTCGATATTCTTCAGCTGACTGGACATTTTTTCACGAACTTCAACGGGCGCGATTACATCGAGAACATCCAGAATTTGTTTGATAACCAACTGGCAAGCAACCACATc                          >  1:17336/1‑250 (MQ=255)                                                                                                                                                                                                                                            gTTTGAAGCAGAGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCAATAAGCGCGATGGCTTTTTGGATTGCCCGCATGGTGACCGGGTCTGCGGCGGCGGGATGGTTAGTAAAGTCGATATTCTTCAGCTGACTGGACATTTTTTCACGAACTTCAACGGGCGCGATTACATCGAGAACATCCAGAATTTGTTTGATAACCAACTGGCAAGCAACCACATCAGAAACCAATTCCTg           <  1:802084/247‑1 (MQ=255)                                                                                                                                                                                                                                               gtaaGCAGAGGTTTTTTTAATTGGATTCACCCTGGTGGGTAAACTTGAGTTCAATAAGTGCGATGGCTTTTTGGATTGCCCTCATGGTGACCGGGTCTGCGGCGGCGGGATGGTTAGTAAAGTCGATATTCTTCAGCGGACTGGACATTTTTTCACGAACTTCAACTGGCGCGATTACATCGAGAACATCCAGAATTTGTTTGATAACCAACTGGCAAGCAACCACATCAGAATCCAATTCCTGATCgtc     <  2:589001/248‑3 (MQ=255)                                                                                                                                                                                                                                                 aaGCAGAGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCAATAAGCGCGATGGCTTTTTGGATTGCCCGCATGGTGACCGGGTCTGCGGCGGCGGGATGGTTAGTAAAGTCGATATTCTTCAGCTGACTGGACATTTTTTCACGAACTTCAACGGGCGCGATTACATCGAGAACATCCAGAATTTGTTTGATAACCAACTGGCAAGCAACCACATCAGAAACCAATTCCTGATCGGCAtt  >  1:410549/1‑251 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                     AACTGAATGATGATTCAACCGTCAATTTCCTGCGCGATCTGGAAAAAGAACAGCAGCATGATGGTCTGTTGCTGCAAACCATTCTTGATGAAGTGCGCAGTGCGAAACTTGCGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCAATAAGCGCGATGGCTTTTTGGATTGCCCGCATGGTGACCGGGTCTGCGGCGGCGGGATGGTTAGTAAAGTCGATATTCTTCAGCTGACTGGACATTTTTTCACGAACTTCAACGGGCGCGATTACATCGAGAACATCCAGAATTTGTTTGATAACCAACTGGCAAGCAACCACATCAGAAACCAATTCCTGATCGGCATT  >  NC_000913/1987253‑1987743

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑