BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,643,679	A→T	L209Q (CTG→CAG)	ydfU ←	protein YdfU

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,643,679	0	A	T	99.1%	324.9 / NA	106	L209Q (CTG→CAG)	ydfU	protein YdfU
Reads supporting (aligned to +/- strand): ref base A (0/1); new base T (59/46); total (59/47)
Fisher's exact test for biased strand distribution p-value = 4.43e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01

Only 100 of 106 total aligned reads displayed.

CCAATCAGATGATGCGGATCGTCGGCTGGCATACCGCAGCAAGCACACGGCTGTGTCTTAACCCAACGTGTGTATTTCTCCTTAACCCAGCGGCGACGTTTAGGCAGCTTCATGAAAGATTCCGGAGACTCTGGATCAACGG‑TGATGCTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCAGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCAGATTTTTTCCGCTGGTAATCCCAGCGAACGACGCGATACAGCCTCAGGTAGTGCATCCACCACCTGATTGCAGACCGCCCACCAGGATAATTCAGCCAAAGATAATTCCCGCTCCTGCGTACCGCTTATTGCGTGACGGATGACGTCAATCACCCATGCTGTCAGATTTTG‑TTGAGCAAGCAGCTCCAGTGATTCCGATGTCTGGT  >  NC_000913/1643433‑1643925

ccAATCAGATGATGCGGATCGTCGGCTGGCATACCGCAGCAAGCACACGGCTGTGTCTTAACCCAACGTGTGTATTTCTCCTTAACCCAGCGGCGACGTTTAGGCAGCTTCATGAAAGATTCCGGAGACTCTGGATCAACGG‑TGATGCTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGctc                                                                                                                                                                                                                                                     <  1:570987/251‑1 (MQ=255)

 cAATCAGATGATGCGGATCGTCGGCCGGCATACCGCAGCAAGCACACGGCTGTGTCTTAACCCAACGAGTGTATTTCTCCTTCACCCCGCGGCGACGTTTAGGCAGCTTCATGAAAAATTCCAGAGACTCTGGATCACAGG‑GGATGCTTACCACCGCCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGGCAGGCCACGGTGCAAGATTTTTTGTGGGTTGTTTCAATATGCTGGTGGCGGTCTGctct                                                                                                                                                                                                                                                    >  2:104751/1‑251 (MQ=255)

  aattcgatgatTCGGATTGTCGGCTGGCCTACCGCAGCAAGCATACGGCTGTGTCTTAACCCAACTTGTGTATTGCTCCTTAACCCAGCGGCGACGTTTAGGCAGCTTCATGAAAGATTCCGGAGACTCTGGATCAACGG‑TGATGCTTACCACCGCCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCtctc                                                                                                                                                                                                                                                   <  2:798947/246‑1 (MQ=255)

     cAGATGATGCGGATCGTCGGCTGGCATACCGCAGCAAGCACACGGCTGTGTCTTAACCCAACGTGTGTATTTCTCCTTAACCCAGCGGCGACGTTTAGGCAGCTTCATGAAAGATTCCGGAGACTCTGGATCAACGG‑TGATGCTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTccc                                                                                                                                                                                                                                                 <  1:503862/250‑1 (MQ=255)

      aGATGATGCGGATCGTCGGCTGGCATACCTCAGCAAGCACACGGCTGTGTCTTAACCCAACGTGTGTATTTCTCCTTAACCCAGCGGCGACGTTTAGGCAGCTTCATGAAAGATTCCGGAGACTCTGGATCAACGG‑TGATGCTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCgg                                                                                                                                                                                                                                               >  1:917727/1‑251 (MQ=255)

          gatgCGGATCGTCGGCTGGCATACCGCAGCAAGCACACGGCTGTGTCTTAACCCAACGTGTGTATTTCTCCTTAACCCAGCGGCGACGTTTAGGCAGCTTCATGAAAGATTCCGGAGACTCTGGATCAACGG‑TGATGCTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTTCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTAc                                                                                                                                                                                                                                            <  1:503161/250‑1 (MQ=255)

               ggATCGCCGGCTGGCATACCGCAGCAAGCACACGGCTGTGTCTTAACCCAACGTGTGTATTTCCCCTTAACCCAGCGGCGACGTTTAGGCAGCTTCATGAAAGATTCCGGAGACTCTGGATCAACGG‑TGATGCTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCTTAAGGCAACGGTTCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTc                                                                                                                                                                                                                                      <  2:226471/251‑1 (MQ=255)

                 aTCGTCGGCTGGCATACCGCAGCAAGCACACGGCTGTGTCTTAACCCAACGTGTGTATTTCTCCTTAACCCAGCGGCGACGTTTAGGCAGCTTCATGAAAGATTCCGGAGACTCTGGATCAACGG‑TGATGCTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGc                                                                                                                                                                                                                                    >  1:306478/1‑251 (MQ=255)

                   cgtcgGCTGGCATACCGCAGCAAGCACACGGCTGTGTCTTAACCCAACGTGTGTATTTCTCCTTAACCCAGCGGCGACGTTTAGGCAGCTTCATGAAAGATTCCGGAGACTCTGGATCAACGG‑TGATGCTTACCACCGTCTTTTCCTGTGGAGATTTTTGTTGCTGGAGGGCGTACGGCAACGGTGAAAGATTTTTTGTGCGTTGTTTCAATATGCTGGGGGCGGGCTGCTCTCCCGGTACGATGTAGCtt                                                                                                                                                                                                                                  >  2:527264/1‑251 (MQ=255)

                    gtcgGCTGGCATACCGCAGCAAGCACACGGCTGTGTCTTAACCCAACGTGTGTATTTCTCCTTAACCCAGCGGCGACGTTTAGGCAGCTTCATGAAAGATTCCGGAGACTCTGGATCAACGG‑TGATGCTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCttt                                                                                                                                                                                                                                 >  1:859762/1‑251 (MQ=255)

                       ggCTGGCATACCGCAGCAAGCACACGGCTGTGTCTTAACCCAACGTGTGTATTTCTCCTTAACCCAGCGGCGACGTTTAGGCAGCTTCATGAAAGATTCCGGAGACTCTGGATCAACGG‑TGATGCTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTcgc                                                                                                                                                                                                                              >  1:631851/1‑251 (MQ=255)

                              agaCCGCAGCAAGCACACGGTGGTGTCTTAAACCAACGTGTGTATTTCTCCTTAACCCAGCGGCGACGTTTAGGCAGCGTCATGAAAGATTCCGGAGACTCTGGATCAACGG‑TGATGCTTACCACCGTCGTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATAGGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTacat                                                                                                                                                                                                                       <  2:146736/249‑2 (MQ=255)

                              aTACCGCAGCAAGCACACGGCTGTGTCTTAACCCAACGTGTGTATTTCTCCTTAACCCAGCGGCGCCGTTTAGGCAGCTTCATGAAAGATTCCGGAGACTCTGGATCAACGG‑TGATGCTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAATATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTTCTCTCCCGGTACGTtttcgctttcgctgttcac                                                                                                                                                                                                                       >  2:523346/1‑244 (MQ=255)

                                accgtagcaagccaacgtgtggttCTTAACCCAACGTGTGTATTGTTCCTTAACCCAGCTGGGACGTTGAGGCAGCTTCATGAAAGATGCCGGAGACTCTGGAGCAACGG‑TGATGCTTACCACCGTCTTTTCCTGTGGTGAGTTTGGTTGCTGGTGGGCGTAAGGCAACGTTGCAAGATTTGTTGTGCGTTGTTGCAATATGCTGGTGGCGGTAAGCGCTCCAGGTGCGATGGCGCCTTCGCGGCACAcc                                                                                                                                                                                                                      <  2:984219/227‑1 (MQ=255)

                                  cGCAGCAAGCACACGGCTGTGTCTTAACCCAACGTGTGTATATCTCCTTAACCCAGCGGCGACGTTTAGGCAGCTTCATGAAAGATTCCGGAGACTCTGGATCAACGG‑TGATGCTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCg                                                                                                                                                                                                                     >  1:362818/1‑249 (MQ=255)

                                    cagcaAGCACACGGCTGTGTCTTAACCCAACGTGTGTATTTCTCCTTAACCCAGCGGCGACGTTTAGGCAGCTTCATGAAAGATTCCGGAGACTCTGGATCAACGG‑TGATGCTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCa                                                                                                                                                                                                                 <  1:770924/251‑1 (MQ=255)

                                             cacGGCTGTGTCTTAACCCAACGTGTGTATTTCTCCTTAACCCAGCGGCGACGTTTAGGCAGCTTCATGAAAGATTCCGGAGACTCTGGATCAACGG‑AGATGCTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCACTATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCAGATTTTTTc                                                                                                                                                                                                        >  1:853079/1‑251 (MQ=255)

                                                ggCTGTGTCTTAACCCAACGTGTGTATTTCTCCTTAACCCAGCGGCGACGTTTAGGCAGCTTCATGAAAGATTCCGGAGACTCTGGATCAACGG‑TGATGCTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCAGATTTTTTCCGc                                                                                                                                                                                                     >  1:436238/1‑251 (MQ=255)

                                                 gCTGTGTCTTAACCCAACGTGTGTATTTCTCCTTAACCCAGCGGCGACGTTTAGGCAGCTTCATGAAAGATTCCGGAGACTCTGGATCAACGG‑TGATGCTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTCAGATGTCGCTTTCGCGGTACACCGAGCAGATTTTTTCCGCt                                                                                                                                                                                                    >  2:307176/1‑251 (MQ=255)

                                                  cTGTGTCTTAACCCAACGTGTGTATTTCTCCTTAACCCAGCGGCGACGTTTAGGCAGCTTCATGAAAGATTCCGGAGACTCTGGATCAACGG‑TGATGCTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCAGATTTTTTCCGCTg                                                                                                                                                                                                   >  1:269864/1‑251 (MQ=255)

                                                    gtgtCTTAACCCAACGTGTGTATTTCTCCTTAACCCAGCGGCGACGTTTCGGCAGCTTCATGAAAGATTCCGGAGACTCTGGATCAACGG‑CGATGCTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCACGATTTTTTGTGCGTTGTTTCACTATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCAGATTTTTTCCGCTGGt                                                                                                                                                                                                 >  1:329121/1‑251 (MQ=255)

                                                     tgtCTTAACCCAACGTGTGTATTTCTCCTTAACCCAGCGGCGACGTTTAGGCAGCTTAATGAAAGATTCCGGAGACTCTGGATCAACGG‑GGATGCTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCAGATTTTTTCCGCTGGt                                                                                                                                                                                                 >  1:571514/1‑250 (MQ=255)

                                                        cTTAACCCAACGTGTGTATTTCTCCTTAACCCAGCGGCGACGTTTAGGCAGCTTCATGAAAGATTCCGGAGACTCTGGATCAACGG‑TGATGCTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCAGATTTTTTCCGCTGGTAATc                                                                                                                                                                                             >  1:28507/1‑251 (MQ=255)

                                                          tAACCCAACGTGTGTATTTCTCCTTAACCCAGCGGCGACGTTTAGGCAGCTTCATGAAAGATTCCGGAGACTCTGGATCAACGG‑TGATGCTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCAGATTTTTTCCGCTGGTAATc                                                                                                                                                                                             <  1:698203/249‑1 (MQ=255)

                                                             cccAACGTGTGTATTTCTCCTTAACCCAGCGGCGACGTTTAGGCAGCTTCATGAAAGATTCCGGAGACTCTGGATCAACGG‑TGATGCTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCAGATTTTTTCCGCTGGTAATCCCAGc                                                                                                                                                                                        <  1:688325/251‑1 (MQ=255)

                                                              ccAACGTTTGTATTTCTCCTTAACCCAGAGGCGACGTTTAGGCAGCTTCATGAAAGATTCCGGAGACTCTGGATCAACGG‑TGAGGCTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCTTGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCATATTTTGTCCGCTGGTAATCCCAGCg                                                                                                                                                                                       <  2:488786/251‑1 (MQ=255)

                                                              caaaCGGGTGTATTTCTCCTTAACCCAGCGGCGACGTTTAGGCAGCTTCATGAAAGATTCCGGAGCCTCTGGATCAACGG‑TGATGCTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTCCGGAGTCGCTTTCGCGGTACACCGAGCAGATTTTTTCCGCTGGTAATCCCAGCg                                                                                                                                                                                       >  2:657412/3‑251 (MQ=255)

                                                                 aCGTGTGTATTTCTCCTTAACCCAGCGGCGACGTTTAGGCAGCTTCATGAAAGATTCCGGAGACTCTGGATCAACGG‑TGATGCTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCAGATTTTTTCCGCTGGTAATCCCAGCGAac                                                                                                                                                                                    <  1:673038/251‑1 (MQ=255)

                                                                     gtgtATTTCTCCTTAACCCAGAGGCGACGTTTTGGCAGCTTCATGAAAGATTCCGGAGACTCTGGATCAACGG‑TGATGCTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTATTTGTGCGTTGTTTCAATATGCTGGTGGCGGTATGCTCTCCAGGTACGATGTCGCTTTCGCGGTACAACGAGCAGCTTTTTTCCGCTGGTAAtacacgcgaatgacg                                                                                                                                                                                >  2:723857/1‑237 (MQ=255)

                                                                       gtATTTCTCCTTAACCCAGCGGCGACTGTTAGGCAGCTTCATGAAAGATTCCGGAGACTCTGGATCAACGG‑TGATGCTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCAGATTTTTTCCGCTTGTAATCCCAGCGAACGAcgc                                                                                                                                                                               <  2:789589/250‑1 (MQ=255)

                                                                              tcCTTAACCCAGCGGCGACGTTTAGGCAGCTTCATGAAAGATTCCGGAGACTCTGGATCAACGG‑TGATGCTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCAGATTTTTTCCGCTGGTAATCCCAGCGAACGACGCGATACAGc                                                                                                                                                                       >  1:280414/1‑251 (MQ=255)

                                                                                cTTAACCCAGCGGCGACGTTTAGGCAGCTTCATGAAAGATTCCGGAGACTCTGGATCAACGG‑TGATGCTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGGTTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCAGGTTTTTTCCGCTGGTAATCCCAGCGCACGACGCGATaaaccat                                                                                                                                                                     >  2:560680/1‑245 (MQ=255)

                                                                                cTTAACCCAGCGGCGACGTTTAGGCAGCTTCATGAAAGATTCCGGAGACTCTGGATCAACGG‑TGATGCTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGCTGTCGCTTTCGCGGTACACCGAGCAGATTTTTTCCGCTGGTAATCCCAGCGAACGACGCGATACAGCCt                                                                                                                                                                     >  2:328201/1‑251 (MQ=255)

                                                                                cTTAACCCAGCGGCGACGTTTAGGCAGCTTCATGAAAGATTCCGGAGACTCTGGATCAACGG‑TGATGCTTAACACCGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCAGATTTTTTCCGCTGGTAATCCCAGCGAACGACGCGATACAGccc                                                                                                                                                                     >  1:605216/1‑250 (MQ=255)

                                                                                   aaCCCAGCGGCGACGTTTAGGCAGCTTCATGAAAGAGTCCGGAGACTCTGGATCAACGG‑TGATGCTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCAGATTTTTTCCGCTGGTAATCCCAGCGAACGACGCGATACAGCCTCAg                                                                                                                                                                  <  2:15862/251‑1 (MQ=255)

                                                                                        aGCGGGGACGTTTAGGCAGCTTAATGAAAGATTCCGGAGACTCTGGATCAACGG‑TGATGCGTACCACCGTCTTTTCCTGTGGTGATTTTTGTGGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCAGATTTTGTCCGCTGGTAATCCCAGCGAACGACGAGATACAGCCTCAGGTAGt                                                                                                                                                             <  2:148422/251‑1 (MQ=255)

                                                                                          cggcgACGTTTAGGCAGCTTCATGAAAGATTCCGGAGATTCTGGATCAACGG‑TGATGCTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGCAACGTTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCAGATTTTTTCCGCTGGTAATCCCAGCGAACGACGCGATACAGCCTCAGGTAGTGc                                                                                                                                                           <  2:663822/251‑1 (MQ=255)

                                                                                           ggcgACGTTTAGGCAGCTTCATGAAAGATTCCGGAGACTCTGGATCAACGG‑TGATGCTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCAGATTTTTTCCGCTGGTAATCCCAGCGAACGACGCGATACAGCCTCAGGTAGTGc                                                                                                                                                           >  1:649877/1‑250 (MQ=255)

                                                                                           ggcgACGTTGAGGCAGCTTCATGAAAGATTCCGGAGACTCTGGATCAACGG‑TGATGCTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCAGATTTTTTCCGCTGGTAATCCCAGCGAACGACGCGATACAGCCTCAGGTAGTGc                                                                                                                                                           >  1:850310/1‑250 (MQ=255)

                                                                                             cgACGTTTAGGCAGCTTCATGAAAGATTCCGGAGACTCTGGATCAACGG‑TGATGCTTACCACCGTCTTTTCATGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGCCACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCAGCTTTTTTCCGCTGGTACTCCCAGCGAACGACGCGAGACAGCCTCAGGGAGTGCATc                                                                                                                                                        >  2:621229/1‑251 (MQ=255)

                                                                                              gACGTTTAGGCAGCTTCATGAAAGATTCCGGAGACTCTGGCTCAAAGG‑TGATGCTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTAGCGGTCCACCGAGCAGATTTTTTCCGCTGGTAATCCCAGCGAACGACGCGATACAGCCTCAGGAAGTGCTTcc                                                                                                                                                       >  2:841172/1‑251 (MQ=255)

                                                                                               aCGTTTAGGCAGCTTCATGAAAGATTCCGGAGACTCTGGATCAACGG‑TGATGCTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCAGATTTTTTCCGCTGGTAATCCCAGCGAACGACGCGATACAGCCTCAGGTAGTGCATccc                                                                                                                                                      >  1:758123/1‑250 (MQ=255)

                                                                                               aCGTTTAGGCAGCTTCATGAAAGATTCCGGAGACTCTGGATCAACGG‑TGATGCTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCAGATTTTTTCCGCTGGTAATCCCAGCGAACGACGCGATACAGCCTCAGGTAGTGCATcca                                                                                                                                                      <  1:275118/251‑1 (MQ=255)

                                                                                                cGTTTAGGCAGCTTCATGAAAGATTCCGGAGACTCTGGATCAACGG‑TGATGCTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTGAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCAGATTTTTTCCGCTGGTAATCCCAGCGAACGACGCGATACAGCCTCAGGTAGTGCATccac                                                                                                                                                     >  1:706679/1‑251 (MQ=255)

                                                                                                 gTTTAGGCAGCTTCATGAAAGATTCCGGAGACTCTGGATCAACGG‑TGATGCTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCAGATTTTTTCCGCTGGTAATCCCAGCGAACGACGCGATACAGCCTCAGGTAGTGCATccacc                                                                                                                                                    >  1:130123/1‑251 (MQ=255)

                                                                                                         aGCTTCATGAAAGATTCCGGAGACTCTGGATCAACGG‑TGATGCTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGCTTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGCTGTCGCTTTCGCGGTACACCGAGCAGATTTTTTCCGCTGGTAATCCCAGCGAACGACGCGATACAGCCTCAGGAAGTTCCTCCACCACCTGAtt                                                                                                                                            >  2:74701/1‑251 (MQ=255)

                                                                                                                 gAAAGATTCCGGAGACTCTGGATCAACGG‑TGATGCTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCAGATTTTTTCCGCTGGTAATCCCAGCGAACGACGCGATACAGCCTCAGGTAGTGCATCCACCACCTGATTGCAGAcc                                                                                                                                     <  1:300977/250‑1 (MQ=255)

                                                                                                                         ccGGAGACTCTGGATCAACGG‑TGATGCTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCAGATTTTTTCCGCTGGTAATCCCAGCGAACGACGCGATACAGCCTCAGGTAGTGCATCCACCACCTGATTGCAGACCGCccacca                                                                                                                             <  1:556742/250‑1 (MQ=255)

                                                                                                                          cGGAGACTCTGGATCAACGG‑TGATGCTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCAGATTTTTTCCGCTGGTAATCCCAGCGAACGACGCGATACAGCCTCAGGTAGTGCATCCACCACCTGATTGCAGACCGCCCACCAgg                                                                                                                           >  2:962468/1‑251 (MQ=255)

                                                                                                                            gcgaCTCTGGATCAACGG‑TGATGCTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGCTGTCGCTTTCGCGGTACACCGAGCAGATTTTTTCCGCTGGTAATCCCAGCGAACGACGCGATACAGCCTCAGGTAGTGCATCCACCACCTGATTGCAGACCGCCCACCAGGAt                                                                                                                         >  2:656916/3‑251 (MQ=255)

                                                                                                                            gagaCGCTGGATCACCGGGTG‑TGCTTACCACCGTCTTTTCCTGTGGTGATTTTTGGTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCAGATTTTTTCCGCTGGTAATCCCAGCGAACGACGCGATACAGCCTCAGGTAGTGCATCCACCACCTGATTCCAGACCGCCCACCAGGAt                                                                                                                         <  2:480761/251‑1 (MQ=255)

                                                                                                                                ctctGGATCAACGG‑TGATGCTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGCAAAGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTccc                                                                                                                                                                                                                                                 >  1:74423/1‑127 (MQ=255)

                                                                                                                                  ctGGATCAACGG‑TGATGCTTACCACCGTCTTTTCCTGAGGTGATTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGAGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTACCGGTACGATGTCGCTTTCGCGGTACACCGAGCAGATTTTTTCCGCGGGTAATCCCAGCGACCGCCCCGATACAGCCTCCGGTAGTGCATCCCCCACCTGATTGCAGACCGCCCCCCAGGATAATTcc                                                                                                                   >  2:599160/1‑250 (MQ=255)

                                                                                                                                     gATCAACGG‑TGATGCTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCAGATTTTTTCCGCTGGt                                                                                                                                                                                                 <  2:528426/170‑1 (MQ=255)

                                                                                                                                     gATCAACGG‑TGATGCTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCAGATTTTTTCCGCTGGt                                                                                                                                                                                                 >  1:528426/1‑170 (MQ=255)

                                                                                                                                      aTCAACGG‑TGATGCTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCAGATTTTTTCCGCTGGTAATCCCAGCGAACGACGCGATACAGCCTCAGGTAGTGCATCCACCACCTGATTGCAGACCGCCCACCAGGATAATTCAGcc                                                                                                                >  1:258987/1‑250 (MQ=255)

                                                                                                                                        cAACGG‑TGATGCTTACCACCGTCTTTTCCTGTGGTGCTTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCAGATTTTTTCCGCTGGTAATCCCAGCGAACGACGCGATACAGCCTCAGGTAGTGCCTCCACCACCTGATTGCAGACCGCCCACCAGGAAAATTCAGCCaa                                                                                                              >  2:728386/1‑250 (MQ=255)

                                                                                                                                            gg‑TGATGCTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGTTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCAGATTTTTTCCGCTGGTAATCCCAGCGAACGACGCGATACAGCCTCAGGTAGTGCATCCACCACCTGATTGCAGACCGCCCACCAGGATAATTCCGCCAAAGAt                                                                                                          >  1:374353/1‑250 (MQ=255)

                                                                                                                                            cggggATGCTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGGAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCAGATTTTTTCCGCTGGTAACCCCAGCGAACGACGCGATACAGCCTCAGGTAGTGCATCCACCACCTGATTGCAGACCGCCCACCAGGATAATTCAGCCAAAGAt                                                                                                          <  2:53979/247‑1 (MQ=255)

                                                                                                                                                 aagCTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCGGGTGGGCGTAAGGCAACGGTGCAAGATTTTTGGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCAGATTTTTTCCGCTGGTAATCCCAGCGAACGACGCGATACAGCCTCAGGTAGTGCATCCACCACCTGATTGCAGACCGCCCACCAGGATAATTCAGCCAAAGATAATTc                                                                                                     <  2:725238/249‑1 (MQ=255)

                                                                                                                                                 aTGCTTACCACCGTCTTTTCCGGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCAGATTTTTTCCGCTGGTAATCCCAGCGAACGACGCGATACAGCCTCAGGTAGTGCATCCACCACCTGATTGCAGACCGCCCACCAGGATAATTCAGCCAAAGATAATTc                                                                                                     <  2:584135/251‑1 (MQ=255)

                                                                                                                                                   gCTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTCCGATGTCGCTTTCGCGGTACACCGAGCAGATTTTTTCCGCTGGTAATCCCAGCGAACGACGCGATACAGCCTCAGGTAGTGCATCCACCACCTGATTGCAGACCGCCCACCAGGATAATTCAGCACAAGATAATTccc                                                                                                   >  1:477218/1‑251 (MQ=255)

                                                                                                                                                    cTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCAGATTTTTTCCGCTGGTAATCCCAGCGAAAGACGCGATACAGCCTCATGAAGTGCATCCACCCCCTGATTGCAGACCGCCCACCCGGATATATCAGCCAAAGATAATTCCCg                                                                                                  >  2:320321/1‑251 (MQ=255)

                                                                                                                                                       accaccGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCAGATTTTTTCCGCTGGTAATCCCAGCGAACGACGCGATACAGCCTCAGGTAGTGCATCCACCACCTGATTGCAGACCGCCCACCAGGATAATTCAGCCAAAGATAATTCCCGCTc                                                                                               <  1:452122/251‑1 (MQ=255)

                                                                                                                                                          cccggcttgtCCTGTGGTGATTTTCGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCAGATTTTTCCCGCTGGTAATCCCAGCGAACGACGCGATACAGCCTCAGGTAGTGCATCCACCACCTGATTGCAGACCGCCCACCAGGATAATTCAGCCAAAGATAATTCCCGCTCCTg                                                                                            <  1:428904/242‑1 (MQ=255)

                                                                                                                                                           ccGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTGAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCAGATTTTTTCCGCTGGTAATCCCAGCGAACGACGCGATACAGCCTCAGGTAGTGCATCCACCACCTGATTGCAGACCGCCCACCAGGATAATTCAGCCAAAGATAATTCCCGCTCCTGc                                                                                           <  2:706679/251‑1 (MQ=255)

                                                                                                                                                           ccGTCTTTACCTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGGTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCAGATTTTTTCCGCTGGTAATCCCAGCGAACGACGCGATACAGCCTCAGGTAGGGCATCCACCACCTTATTGCAGACCGCCCACCAGGATAATTCAGACAAAGATAATTCCCGCTCCTGc                                                                                           >  2:514005/1‑251 (MQ=255)

                                                                                                                                                                  ttCCTGTGGTGATTTTTTTTGCTGGTGGGGGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCAGATTTTTTCCGCTGGTAATCCCAGCGAACGACGCGATACAGCCTCAGGTAGTGCATCCACCACCTGATTGCAGACCGCCCACCAGGATAATTCAGCCAAAGATAATTCCCGCTCCTGCGTACCg                                                                                     <  2:27534/250‑1 (MQ=255)

                                                                                                                                                                     cTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCAGATTTTTTCCGCTGGTAATCCCAGCGAACGACGCGATACAGCCTCAGGTAGTGCATCCACCACCTGATTGCAGACCGCCCACCAGGATAATTCAGCCAAAGATAATTCCCGCTCCTGCGTACCGCtt                                                                                  >  1:577927/1‑250 (MQ=255)

                                                                                                                                                                            gATTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCAGATTTTTTCCGCTGGTAATCCCAGCGAACGACGCGATACAGCCTCAGGTAGTGCATCCACCACCTGATTGCAGACCGCCCACCAGGATAATTCAGCCAAAGATAATTCCCGCTCCTGCGTACCGCTTATTGCGtt                                                                          >  2:498160/1‑250 (MQ=255)

                                                                                                                                                                                tttGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCAGATTTTTTCCGCTGGTAATCCCAGCGAACGACGCGATACAGCCTCAGGTAGTGCATCCACCACCTGATTGCAGACCGCCCACCAGGATAATTCAGCCAAAGATAATTCCCGCTCCTGCGTACCGCTTATTGCGTGACgg                                                                      >  1:705930/1‑251 (MQ=255)

                                                                                                                                                                                    ttgCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCAGATTTTTTCCGCTGGTAATCCCAGCGAACGACGCGATACAGCCTCAGGTAGTGCATCCACCACCTGATTGCAGACCGCCCACCAGGATAATTCAGCCAAAGATAATTCCCGCTCCTGCGTACCGCTTATTGCGTGACGGATgc                                                                  >  1:516245/1‑250 (MQ=255)

                                                                                                                                                                                     tgCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCAGATTTTTTCCGCTGGTAATCCCAGCGAACGACGCGATACAGCCTCAGGTAGTGCATCCACCACCTGATTGCAGACCGCCCACCAGGATAATTCAGCCAAAGATAATTCCCGCTCCTGCGTACCGCTTATTGCGTGACGGATGa                                                                  <  1:261682/250‑1 (MQ=255)

                                                                                                                                                                                     ggCTGGGGGGCGTAAGGCAACGGTGCAATATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTTCTCTCCCGTGACGATGTCGCTGTCGCGGTCCACCGAGCAGATTTTTTCCGCTGGTAATCCCAGCGAACGACTCTATCCAGCCTCAGGTATTGCATCCACCACCTTATTGCAGACCTCCCACCAGGATAATTCAGCCAAATATAATTCCCGCTCCTGCGTACCGCTTATTGCGTGACGGATGAc                                                                 <  2:329121/250‑1 (MQ=255)

                                                                                                                                                                                       cTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCAGATTTTTTCCGCTGGTAATCCCAGCGAACGACGCGATACAGCCTCAGGTAGTGCATCCACCACCTGATTGCAGACCGCCCACCAGGATAATTCAGCCAAAGATAATTCCCGCTCCTGCGTACCGCTTATTGCGTGACGGATGACGt                                                               >  1:822013/1‑251 (MQ=255)

                                                                                                                                                                                         ggtggGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCAGATTTTTTCCGCTGGTAATCCCAGCGAACGACGCGATACAGCCTCAGGTAGTGCATCCACCACCTGATTGCAGACCGCCCACCAGGATAATTCAGCCAAAGATAATTCCCGCTCCTGCGTACCGCTTATTGCGTGACGGATGACGTCa                                                             <  1:74701/251‑1 (MQ=255)

                                                                                                                                                                                          gggtgCGTAAGGCAACGTTGCAAGATTTCTTGTGCGTTGTCTCAAGAGGCTGGCGGCGGGCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCAGATTTTTTCCGCTGGTAATCCCAGCGACCGACGCGATACAGCCTCAGGTAGTGCATCCACCTCCTGATTGCAGACCGCCCACCAGTATAATTCAGCCAAAGATAATTCCCGCTCCTGCGTACCGCTTATTGCGTGACGGATGACGTCaa                                                            <  2:356216/247‑1 (MQ=255)

                                                                                                                                                                                           tggGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGATCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCAGATTTTTTCCGCTGGTAATCCCAGCGAACGACGCGATACAGCCTCAGGTAGTGCATCCACCACCTGATTGCAGACCGCCCACCAGGATAATTCAGCCAAAGATAATTCCCGCTCCTGCGTACCGCTTATTGCGTGACGGATGACGTCAAt                                                           >  2:928009/1‑251 (MQ=255)

                                                                                                                                                                                            gggCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCAGATTTTTTCCGCTGGTAATCCCAGCGAACGACGCGATACAGCCTCAGGTAGTGCATCCACCACCTGATTGCAGACCGCCCACCAGGATAATTCAGCCAAAGATAATTCCCGCTCCTGCGTACCGCTTATTGCGTGACGGATGACGTCAAt                                                           <  2:501473/250‑1 (MQ=255)

                                                                                                                                                                                               cggaaGGCAACGGTGAAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCAGATTTTTTCCGCTGGTAATCCCAGCGAACGACGCGATACAGCCTCAGGTAGTGCATCCACCACCTGATTGCAGACCGCCCACCAGGATAATTCAGCCAAAGATAATTCCCGCTCCTGCGTACCGCTTATTGCGTGACGGATGACGTCAATCAcc                                                       <  2:151479/248‑1 (MQ=255)

                                                                                                                                                                                                ggtaGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATAGGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCAGATTTTTTCCGCTGGTAATCCCAGCGAACGACGCGATACAGCCTCAGGTAGTGCATCCACCACCTGATTGCAGACCGCCCACCAGGATAATTCAGCCAAAGATAATTCCCGCTCCTGCGTACCGCTTATTGCGTGACGGATGACGTCAATCAccc                                                      <  2:436238/248‑1 (MQ=255)

                                                                                                                                                                                                  aaGGCAACGGTGCAAGATTTTTTGTGAGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCAGATTTTTTCCGCTGGTAATCCCAGCGAACGACGCGATACAGCCTCAGGTAGTGCATCCACCACCTGATTGCAGACCGCCCACCAGGATAATTCAGCCAAAGATAATTCCAGCTCCTGCGTACCGCTTATTGCGTGACGGATGACGTCAATCACCCAt                                                    >  1:26251/1‑251 (MQ=255)

                                                                                                                                                                                                   aGGCAACGGTGCTAGATTTTTTGTGCCTTGTTTCAATATGCTGGGTGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCAGATTTTTGCCGCTGGTAATCCCAGCGAACGACGCGATACAGCCTCAGGGAGTGCATCCACCACCTGATTGCAGACCGCCCACCAGGATAATTCAGCCAAAGATAATTCCCGCTCCTGCGTACCGCTTATTGCGTGACGTATGACGTCAATCACCCATg                                                   <  2:111092/251‑1 (MQ=255)

                                                                                                                                                                                                     gCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCAGCTTTTTTCCGCTGGTCATCCCAGCGAACGACGCGATACAGCCTCAGGTAGTGCATCCACCACCTGATTGCAGACCGACCACCAGGATAATTCAGCCAAAGATAATTCCCGCTCCTGCGTACCGCTTATTGCGTGACGGATGACGTCAATCACCCAtcct                                                 >  2:445019/1‑248 (MQ=255)

                                                                                                                                                                                                       aaCGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGGACACCGAGCAGATTTTTTCCGCTGGTAATCCCAGCGAACGACGCGATACAGCCTCAGGTAGTGCATCCACCACCTGATTGCAGACCGCCCACCAGGATAATTCAGCCAAAGATAATTCCCGCTCCTGCGTACCGCTTATTGCGTGACGGATGACGTCAATCACCCATGCTGt                                               <  1:205658/251‑1 (MQ=255)

                                                                                                                                                                                                          ggTGCAAGAGTTTTTGTGCGTTGTTGCATTATGCTGGTGGCGGGCTGCTCTCCCGGTACGAGGTCGCTTGCGCGGTACACCGAGCAGATTTTTTCCGCTGGTAATCCCAGCGAACGACGCGATACAGCCTCAGGTAGTGCATCCACCACCTGCTTGCAGACCGCCCACCAGGATAATTCAGCCAAAGATAATTCCCGCTCCTGCGTACCGCTTATTGCGTGACGGATGACGTCAATCCCCCATGCTGTCAg                                            <  2:306478/251‑1 (MQ=255)

                                                                                                                                                                                                            ggCAAGATTTTTTGTGCGGTGTTTCAATATGCTGGTGGCGTGCTTCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCAGATTTTTTCCGCTGGTAACCCCAGCGAACGACGCGATACAGCCTCAGGTAGTGCATCCACCACCTGATTGCAGACCGCCCACCAGGATAATTCAGCCAAAGATAATTCCCGCTCCTGCGTACCGCTTATTGCGTGACGGATGACGTCAATCACCCATGCTGTCAGAt                                          <  2:362924/250‑1 (MQ=255)

                                                                                                                                                                                                               aaGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCAGATTTTTTCCGCTGGTAATCCCAGCGAACGACGCGATACAGCCTCAGGTAGTGCATCCACCACCTGATTGCAGACCGCCCACCAGGATAATTCAGCCAAAGATAATTCCCGCTCCTGCGTACCGCTTATTGCGTGACGGATGACGTCAATCACCCATGCTGTCAGAtttt                                       <  1:328201/251‑1 (MQ=255)

                                                                                                                                                                                                                aGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCAGATTTTTTCCGCTGGTAATCCCAGCGAACGACGCGATACAGCCTCAGGTAGTGCATCCACCACCTGATTGCAGACCGCCCACCAGGATAATTCAGCCAAAGATAATTCCCGCTCCTGCGTACCGCTTATTGCGTGACGGATGACGTCAATCACCCATGCTGTCAGATTttg                                      >  1:118771/1‑251 (MQ=255)

                                                                                                                                                                                                                  aTTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCAGATTTTTTCCGCTGGTAATCCCAGCGAACGACGCGATACAGCCTCAGGTAGTGCATCAACCACCTGATTGCAGACCGCCCACCAGGATAATTCAGCCCAAGAAAATTCCCGCTCCTGCGTACCGCTTATTGCGAGACGGATGACGTCAATCACCCATGCTGTCAGATTttg‑tt                                   >  2:583470/1‑251 (MQ=255)

                                                                                                                                                                                                                          tgCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCAGATTTTTTCCGCTGGTAATCCCAGCGAACGACGCGATACAGCCTCAGGTAGTGCATCCACCACCTGATTGCAGACCGCCCACCAGGATAATTCAGCCAAAGATAATTCCCGCTCCTGCGTACCGCTTATTGCGTGACGGATGACGTCAATCACCCATGCTGTCAGATTTTG‑TTGagcaagc                           >  2:844602/1‑251 (MQ=255)

                                                                                                                                                                                                                          tgCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCAGATTTTTTCCGCTGGTAATCCCAGCGAACGACGCGATACAGCCTCAGGTAGTGCATCCACCACCTGATTGCAGACCGCCCACCAGGATAATTCAGCCAAAGATAATTCCCGCTCCTGCGTACCGCTTATTGCGTGACGGATGACGTCAATCACCCATGCTGTCAGATTTTG‑TTGagcaagc                           >  1:514471/1‑251 (MQ=255)

                                                                                                                                                                                                                            cGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCAGATTTTTTCCGCTGGTAATCCCAGCGAACGACGCGATACAGCCTCAGGTAGTGCATCCACCACCTGATTGCAGACCGCCCACCAGGATAATTCAGCCAAAGATAATTCCCGCTCCTGCGTACCGCTTATTGCGTGACGGATGACGTCAATCACCCATGCTGTCAGATTTTG‑TTGagcaagc                           >  1:216323/1‑249 (MQ=255)

                                                                                                                                                                                                                                  ttCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCAGATTTTTTCCGCTGGTAATCCCAGCGAACGACGCGATACAGCCTCAGGTAGTGCATCCACCACCTGATTGCAGACCGCCCACCAGGATAATTCAGCCAAAGATAATTCCCGCTCCTGCGTACCGCTTATTGCGTGACGGATGACGTCAATCACCCATGCTGTCAGATTTTG‑TTGAGCAAGCAGCTcc                     >  1:167464/1‑249 (MQ=255)

                                                                                                                                                                                                                                             ggtggCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCAGATTTTTTCCGCTGGTAATCCCAGCGAACGACGCGATACAGCCTCAGGTAGTGCATCCACCACCTGATTGCAGACCGCCCACCAGGATAATTCAGCCAAAGATAATTCCAGCTCCTGCGTACCGCTTATTGCGTGACGGATGACGTCAATCACCCATGCTGTCAGATTTTG‑TTGAGCAAGCAGCTCCAGTGATTCCGAt         <  1:315481/250‑1 (MQ=255)

                                                                                                                                                                                                                                             ggtggCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGAAAACCGAGCAGATTTTTTCCGCTGGTAATCCCAGCGAACGCCGCGATACAGCCTCAGGTAGTGCATCCACCACATGATTGCAGACCGCCCACCAGGATAATTCAGCCAAAGAAAATTCCCGCTCCTGCGTAACGCTTATGACGTGACGGTAGACGTAAATCACCCACGAGTTCAGATTTTGGTTG‑GCATGAAGACCCAGTGATTCCGATg        >  2:384886/1‑251 (MQ=255)

                                                                                                                                                                                                                                              gtggCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCAGATTTTTTCCGCTGGTAATCCCAGCGAACGACGCGATACAGCCTCAGGTAGTGCATCCACCACCTGATTGCAGACCGCCCACCAGGATAATTCAGCCAAAGATAATTCCCGCTCCTGCGTACCGCTTATTGCGTGACGGATGACGTCAATCACCCATGCTGTCAGATTTTG‑TTGAGCAAGCAGCTCCAGTGATTCCGATGt       <  2:442830/251‑1 (MQ=255)

                                                                                                                                                                                                                                               ttgaggTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTCCACCGAGCAGATTTTTTCCGCTGGTAATCCCAGCGCACGACGCGATACAGCCTCAGGTAGTGCATCCACCACCTGATTGCAGACCGCCCACCAGGATAATTCAGCCAAAGATAATTCCCGCTCCTGCGTACCGCTTATTGCGTGACGGATGACGTCAATCCCCCATGCTGTCAGATTTTG‑TTGAGCACGCAGCTCCAGTGATTCCGAtcac      >  2:201292/5‑248 (MQ=255)

                                                                                                                                                                                                                                                 gCGGTCTGCTCTCCCGGTACGCTGTCGCTTTCGCGGTACACCGCGCAGATTTTTTCCGCTGGTAATCCCAGCGAACGACGCGATACAGCCTCAGGTAGTGCATCCACCACCTGATTGCAGACCGCCAACCAGGATAATTCAGCCAAAGATAATTCCCGCTCCTGCGTACCGCTTATTGCGTGACGGCTGACGTCAATCACCCATGCTGTCAGATTTTG‑TTGAGCAAGCAGCTCCAGTGATTCCGATGTCTg    >  2:452055/1‑251 (MQ=255)

                                                                                                                                                                                                                                                   ggTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCAGATTTTTTCCGCTGGTAATCCCAGCGAACGACGCGATACAGCCTCAGGTAGTGCATCCACCACCTGATTGCAGACCGCCCACCAGGATAATTCAGCCAAAGATAATTCCCGCTCCTGCGTACCGCTTATTGCGTGACGGATGACGTCAATCACCCATGCTGTCAGATTTTG‑TTGAGCAAGCAGCTCCAGTGATTCCGATGTCTGGt  >  1:817068/1‑251 (MQ=255)

CCAATCAGATGATGCGGATCGTCGGCTGGCATACCGCAGCAAGCACACGGCTGTGTCTTAACCCAACGTGTGTATTTCTCCTTAACCCAGCGGCGACGTTTAGGCAGCTTCATGAAAGATTCCGGAGACTCTGGATCAACGG‑TGATGCTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCAGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCAGATTTTTTCCGCTGGTAATCCCAGCGAACGACGCGATACAGCCTCAGGTAGTGCATCCACCACCTGATTGCAGACCGCCCACCAGGATAATTCAGCCAAAGATAATTCCCGCTCCTGCGTACCGCTTATTGCGTGACGGATGACGTCAATCACCCATGCTGTCAGATTTTG‑TTGAGCAAGCAGCTCCAGTGATTCCGATGTCTGGT  >  NC_000913/1643433‑1643925

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`