Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,301,992	A→T	N271Y (AAT→TAT)	oppA →	oligopeptide ABC transporter periplasmic binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,301,992	0	A	T	93.2%	254.1 / ‑5.1	92	N271Y (AAT→TAT)	oppA	oligopeptide ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base A (3/3);  new base T (57/25);  total (61/31)
Fisher's exact test for biased strand distribution p-value = 3.78e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

AACTTCACCGGTGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCG‑TTATTAACCAGGTAACC‑TATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACC‑GCTACC‑GTAGTGGTGAAATCGACAT‑GACGAATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTG‑CAC‑TT‑ACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGTTACACTCCACCGTATACTGATGGCGCAAA  >  NC_000913/1301748‑1302236                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                        aaCTTCACCGGTGCCAAAAGCCGCTATCGAGAACTTCGGCGAACAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCG‑TTCTTAACCAGGTAACC‑TATTTGCCTATTGCTTCTGAAGTTACCGTTGTCAACC‑GCTACC‑GTAGTGGTGAAATCGACAT‑ACCGAATAAc                                                                                                                                                                                                                                                    >  2:690927/1‑250 (MQ=255) aaCTTCACCGGTGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCG‑TTATTAACCAGGTAACC‑TATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACC‑GCTACC‑GTAGTGGTGAAATCGCCAT‑GACGTATAAc                                                                                                                                                                                                                                                    >  1:814265/1‑250 (MQ=255)           gTGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCG‑TTATTAACCAGGTAACC‑TATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACC‑GCTACC‑GTAGTGGTGAAATCGACAT‑GACGTATAACAGCATGCCGAt                                                                                                                                                                                                                                         <  2:930227/251‑1 (MQ=255)            ggCCAAACGCCGCTATCGAGACAATAGGCGAAAAATGGACCCAGCCTGGTAATACCGTCACCAACGTTGCCTATACCTCAAAAGTATGGGTCGTAATCGCACTAATCGTTCTTGAACGTATCCCGCCCTACTGGACCAACGCGAAAACCG‑TTATTAACCAGGTAACC‑TATTTGCCTATTGCTTCTGAAGTTACCGATGCCACCC‑TCGTCC‑GTAGTGTTGAAAGCGACAT‑GACGtatacctgcattacgatc                                                                                                                                                                                                                                        <  2:76415/250‑15 (MQ=255)             gCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCG‑TTATTAACCAGGTAACC‑TATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACC‑GCTACC‑GTAGTGGTGAAATCGACAT‑GACGTATAACAGCATGCCGATc                                                                                                                                                                                                                                        <  1:518315/250‑1 (MQ=255)               cAAAAGCCGCTATCGAGCAATTCGGCGGAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCG‑TTATTAACCAGGTAACC‑TATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACC‑GCTACC‑GTAGTGGTGAAATCGACAT‑GCCGTATAACAGCATGCCGATCGa                                                                                                                                                                                                                                      >  2:213417/1‑250 (MQ=255)                aaaaGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCG‑TTATTAACCAGGTAACC‑TATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACC‑GCTACC‑GTAGTGGTGAAATCGACAT‑GACGTATAACAGCATGCCGATCGAAt                                                                                                                                                                                                                                    <  1:62389/251‑1 (MQ=255)                   ggcctcTATCGAGAAATTGGGCGAAAAGTGGGCCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAATAGAATGGGTCGTAAACGAACGAATCGTGCGTGAACGCAGCCCGACCTACTGGAATAACGCGAAAACCG‑TTATTAACCAGGGAACC‑TATTTGCCTAGTGCATCTGAAGTCACCGATGTCAACC‑GCTACC‑GGAGTGGTGAAATCGATAT‑GACGTATAACATTATGCCGTTCGAAtttt                                                                                                                                                                                                                                 <  2:684726/246‑3 (MQ=255)                     ccGCTAGCGAAAAAATAGGCGAAAAATGGACCCAACCTGGTAATATCGTCACCAACGGGGCCTATGCCTTAAAAGATTGGGTCGTAAACGAACGAAGCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAATCCG‑TTATTAACCAGGTAACC‑TATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACC‑GCTACC‑GTAGTGGTGAAATCGACAT‑GACGTATAACAGCATGCCGATCGAATTGttt                                                                                                                                                                                                                               <  2:292232/251‑2 (MQ=255)                           ccGAGAAATTCGTCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCG‑TTATTAACCAGGTAACC‑TATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACC‑GCTACC‑GTAGTGGTGAAATCGACAT‑GACGTATAACAGCATGCCGATCGAATTGTTCCAGAAg                                                                                                                                                                                                                         <  2:621136/250‑1 (MQ=255)                              agaAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCATCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCG‑TTATTAACCAGGTAACC‑TATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACC‑GCTACC‑GTAGTGGTGAAATCGACAT‑GACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTg                                                                                                                                                                                                                      >  1:203527/1‑251 (MQ=255)                                aaaTTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCG‑TTATTAACCAGGTAACC‑TATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACC‑GCTACC‑GTAGTGGTGAAATCGACAT‑GACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGa                                                                                                                                                                                                                     >  2:301621/1‑250 (MQ=255)                                         gataaaTGGGCCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGAATGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGAGAAAACCG‑TTATTAACCAGGTAACC‑TATTTGCCTATTGCTTCTGACGTTACCGATGTCACCC‑GCTACC‑GTAGTGGTGAAATCGACAT‑GACGTATAACAGCATGCCGATCGAATTGGTACAGAAGCTGAAAAAaggat                                                                                                                                                                                                            >  2:876683/4‑247 (MQ=255)                                         gAAAAATGGACCCAGCCTGGTAATAACGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCG‑TTATTAACCAGGTAACC‑TATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACC‑GCTACC‑GTAGTGGTGAAATCGACAT‑GACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGAt                                                                                                                                                                                                           <  2:476190/251‑1 (MQ=255)                                            acaTGGAACCAGCCTGGTAATATCGTCCCCACCGGTGCCTATCCCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCG‑TTATTAACCAGGGAACC‑TATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACC‑GCTACC‑ATAGTGGTGACATCGACAT‑GACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGCAAAAAGAGATccc                                                                                                                                                                                                        >  2:110103/3‑251 (MQ=255)                                                     cAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCG‑TTATTAACCAGGTAACC‑TATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACC‑GCTACC‑GTAGTGGTGAAATCGACAT‑GACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGt                                                                                                                                                                                               >  1:797929/1‑251 (MQ=255)                                                         cTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCG‑TTATTAACCAGGTAACC‑TCTTTGCCTATTGCTTCTGAAGTTACCGATGTCAACC‑GCTACC‑GTAGTGGTGAAGTCGACAT‑GACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGAACCCGGACGAAGTTccc                                                                                                                                                                                           >  2:994248/1‑249 (MQ=255)                                                          aggTAATATCGTCACCAACGGTTCCTATAACTTCCAAGTTTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACATACTGTAACAACGAGAAAACCG‑TTATTAACAAGGTAACC‑TATTTGCCTATTGCTTCTGAAGTAACCAATGTAAACC‑GCTACC‑GTAGTGGAGAAATCGACAT‑GACGAATCACAGCAAGCCGATCGACTTGTTCCAGAACATGAAACAAGAGATCGCGGACCAAGtgaac                                                                                                                                                                                           >  2:353178/2‑246 (MQ=255)                                                              aaTATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCG‑TTATTAACCAGGTAACC‑TATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACC‑GCTACC‑GTAGTGGTGAAATCGACAT‑GACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGa                                                                                                                                                                                      <  1:541428/251‑1 (MQ=255)                                                                tatCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAAAGCGAAAACCG‑ATATTAAACAGGTAACC‑TATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACC‑GCTACC‑GTAGTGGTGAACTCGACAT‑GACGTATAACAGCATGCCGATCGAATTGTTCAAGAAGCTGAAAAAAGAGATCCCGGACGacgtcaacggtgatc                                                                                                                                                                                    >  2:431302/1‑237 (MQ=255)                                                                 aacGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCG‑TTATTAACCAGGTAACC‑TATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACC‑GCTACC‑GTAGTGGTGAAATCGACAT‑GACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATc                                                                                                                                                                                    <  2:239491/248‑1 (MQ=255)                                                                    gTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCG‑TTATTAACCAGGTAACC‑TATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACC‑GCTACC‑GTAGTGGTGAAATCGACAT‑GACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCAt                                                                                                                                                                                 >  1:630334/1‑250 (MQ=255)                                                                     tCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCG‑TTATTAACCAGGTAACC‑TATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACC‑GCTACC‑GTAGTGGTGAAATCGACAT‑GACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATAc                                                                                                                                                                               >  1:749362/1‑251 (MQ=255)                                                                            cGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGACCGACCTACTGGAACAACGCGACAACAG‑TTATTAACCAGGTAACC‑TATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACC‑GCTACC‑GTAGTGGTGAAGTCGACAT‑GACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTG‑cc                                                                                                                                                                       >  1:940661/1‑250 (MQ=255)                                                                                gCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCG‑TTATTAACCAGGTAACC‑TATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACC‑GCTACC‑GTAGTGGTGAAATCGACAT‑GACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTG‑CAC‑tt                                                                                                                                                                   >  1:360829/1‑250 (MQ=255)                                                                                 ccTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCG‑TTATTAACCAGGTAACC‑TATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACC‑GCTACC‑GTAGTGGTGAAATCGACAT‑GACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTG‑CAC‑TT‑Ac                                                                                                                                                                >  1:775419/1‑251 (MQ=255)                                                                                 ccTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCG‑TTATTAACCAGGTAACC‑TATTTGCCCATTGCTTCTGAAGTTACCGATGTCAACC‑GCTACC‑GTAGTGGTGAAATCGACAT‑GACGAATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCAAGTTGATCCATACCTGAG‑CAC‑TT‑Ac                                                                                                                                                                >  2:300215/1‑251 (MQ=255)                                                                                  cTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCG‑TTATTAACCAGGTAACC‑TATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACC‑GCTACC‑GTAGTGGTGAAATCGACAT‑GACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTG‑CAC‑TT‑ACt                                                                                                                                                               >  1:704647/1‑251 (MQ=255)                                                                                    ataCCTTAAAAGATTGGGTCGCAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCG‑TTATTAACCAGGTAACC‑TATTTGCCTATTGCTTCTGCAGTTACCGATGTCAACC‑GCTACC‑GTAGTGGTGAAATCGACAT‑GACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTG‑CAC‑TT‑ACTAt                                                                                                                                                             >  1:385093/1‑251 (MQ=255)                                                                                     taCCTTAAAAGCTTGGGTCGTAAACGAACGACTCGTTCTTGAACGCAGCCCGTCCTACTGGAACAACGAGAAAACCG‑TTATTAACCAGGTAACC‑TATTTGCCTATGGCTTCTGAAGTTACCGATGTCAACC‑GCTACC‑GTAGTGGTGAAATCGACAT‑GACGTATAACAGCATGCCGATCGAATTGTTCCAGAGGCTGAAAACAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTG‑‑ACATT‑ACTAtt                                                                                                                                                            >  1:106515/1‑251 (MQ=255)                                                                                      aCCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCG‑TTATTAACCAGGTAACC‑TATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACC‑GCTACC‑GTAGTGGTGAAATCGACAT‑GACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTG‑CAC‑TT‑ACTATTa                                                                                                                                                           <  1:690927/251‑1 (MQ=255)                                                                                           aaaaGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCG‑TTATTAACCAGGTAACC‑TATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACC‑GCTACC‑GTAGTGGTGAAATCGACAT‑GACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTG‑CAC‑TT‑CCTATTACGaa                                                                                                                                                       >  2:217292/1‑250 (MQ=255)                                                                                             aaGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCG‑TTATTAACCAGGTAACC‑TATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACC‑GCTACC‑GTAGTGGTGAAATCGACAT‑GACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTG‑CAC‑TT‑ACTATTACGAAAtt                                                                                                                                                    >  1:781698/1‑251 (MQ=255)                                                                                             aaGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCG‑TTATTAACCAGGTAACC‑TATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACC‑GCTACC‑GTAGGGGTGAAATCGACAT‑GACGGATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTTATCCATACCTGTG‑CAC‑TT‑ACTATGACGAAAtt                                                                                                                                                    <  2:917150/251‑1 (MQ=255)                                                                                                         tAAACGAACGAATCGTTATTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCG‑TTATTAACCAGGTAACC‑TATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACC‑GCTACC‑GTAGTGGTGAAATCGACAT‑GACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAACGAGATCCCGGACGCAGTTCACGTTGATCCATACCTGTG‑ACC‑TT‑ACTATTACGAAATTAACAACCCGaa                                                                                                                                         >  1:846939/1‑250 (MQ=255)                                                                                                          aaaCGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCG‑TTATTAACCAGGTAACC‑TATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACC‑GCTACC‑GTAGTGGTGAAATCGACAT‑GACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTG‑CAC‑TT‑ACTATTACGAAATTAACAACCAGAAAc                                                                                                                                       <  1:943079/251‑1 (MQ=255)                                                                                                             cgaacgaaTCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCG‑TTATTAACCAGGTAACC‑TATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACC‑GCTACC‑GTAGTGGTGAAATCGACAT‑GACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTG‑CAC‑TT‑ACTATTACGAAATTAACAACCAGAAACCGc                                                                                                                                    >  1:716702/1‑251 (MQ=255)                                                                                                               aaagaaTCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCG‑TTATTAACCAGGTAACC‑TATTTGCCTATTGCTTCTGAAGTTACCGCTGTCAACC‑GCTAAC‑GGAGTGGTGAAATCGACAT‑GACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCAGAG‑CAC‑TT‑ACTATTACGAACTTAACAACAAGAAACCGcc                                                                                                                                   >  2:585159/4‑250 (MQ=255)                                                                                                                  gaaTCGTTCTTGAACGCAGCCCGACCTACTGGAACCACGCGAAAACCG‑TTATTAACCAGGTAACC‑TATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACC‑GCTACC‑GTAGTGGTGAAATCGACAT‑GACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTG‑CAC‑TT‑ACTATTACGAAATTAACAACCAGAAACCGCCATTc                                                                                                                               >  2:389762/1‑251 (MQ=255)                                                                                                                  gaaTCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCG‑TTATTAACCAGGTAACC‑TATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACC‑GCTACC‑GTAGTGGTGAAATCGACAT‑GACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTG‑CAC‑TT‑ACTATTACGAAATTAACAACCAGAAACCGCCATTc                                                                                                                               >  1:22443/1‑251 (MQ=255)                                                                                                                    aTCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCG‑TTATTAACCAGGTAACC‑TATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACC‑GCTACC‑GTAGTGGTGAAATCGACAT‑GACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTG‑CAC‑TT‑ACTATTACGAAATTAACAACCAGAAACAGCCATTCa                                                                                                                              >  1:169133/1‑250 (MQ=255)                                                                                                                      cGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCG‑TTATTAACCAGGTAACC‑TATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACC‑GCTACC‑GTAGTGGTGAAATCGACAT‑GACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTG‑CAC‑TT‑ACTATTACGAAATTAACAACCAGAAACCGCCATTCAACg                                                                                                                           >  1:40762/1‑251 (MQ=255)                                                                                                                         tCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCG‑TTATTAACCAGGTAACC‑TATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACC‑GCTACC‑GTAGTGGTGAAATCGACAT‑GACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATCCCTGTG‑CAC‑TT‑ACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGAt                                                                                                                         <  1:954318/250‑1 (MQ=255)                                                                                                                          tttGAACGCAGCCCGTCTTACTGGAAAAACGCGAAAACCG‑TTATTAACCAGTTAACC‑TATTTGCCTATTTCTTCTGAAGTTACCGACGTAAACC‑GCTCCC‑GTAGTGGTGAAATCGACAT‑GCCGTATAACAGCATCCCGATCGAATTGTTCTAGAATCTGAAAAAAGAGTTCCCGGACGAAGTTCACGTTGATCCATACCTTTG‑CAC‑TT‑TCTATTACGAATTTAACAACCAGAAACCGCCTTTCAACGAtg                                                                                                                        <  2:419111/249‑1 (MQ=255)                                                                                                                               aCGCAGCCCGACCTACTGGAACAACGCGAAAACCG‑TTATTAACCAGGTAACC‑TATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACC‑GCTACC‑GTAGTGGTGAAATCGACAT‑GACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTG‑CAC‑TT‑ACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGAtgtgcgtg                                                                                                                  >  1:735081/1‑251 (MQ=255)                                                                                                                                  cAGCCCTACCTACTGGAAAAACGCGAAAACCG‑TTATTAACCAGGTACCC‑TATTTGCCTATTTCTTCTGAATTTACCGATGGCAACC‑GCTCCC‑GTAGTGGTGAAATCGACAT‑GACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACAAAGTTCACGTTGATCCATACCTGTG‑CAC‑TT‑ACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGAtgtgcgtgtgc                                                                                                               <  2:977989/251‑1 (MQ=255)                                                                                                                                  cAGCCCGCCCTACTGGAACAACGCGAAACCCT‑TTATTAACCAGGTAACC‑TTTTTGCCTATTGCTTCTGAAGTTACGGATGTCACCC‑GCTACC‑GTAGTGGTGAAATCGACAG‑GACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTTTG‑CAC‑TT‑ACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGAtgtgcgtgtgc                                                                                                               <  2:158592/251‑1 (MQ=255)                                                                                                                                  cAGCCCGACCTACTGGAACAACGCGAAACCCG‑TTATTAACCAGG‑AACCATATTTGCTTATTGCTTCTGAAGTTACCAATGTCAACC‑GCTACC‑GTAGTGGTGAAATCGAAAT‑GACGAATAACAGCATGCCGAGCGAATTGTTCCAGAAGCAGAAAAAAGAGATCCCGGAAGAAGTTCACGGTGACCCATTCCTGTG‑CAC‑TT‑ACTATTGCGAAAATAACAACAAGAAACCGCCATTCAACGAtgtgcgtgtgc                                                                                                               <  2:21845/251‑1 (MQ=255)                                                                                                                                   aGCCCGACCTACTGGAACAACGCGAAAACCG‑TTATTAACCAGGTAACC‑TATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACC‑GCTACC‑GTAGTGGTGAAATCGACAT‑GACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTG‑CAC‑TT‑ACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGAtgtgcgtgtgcg                                                                                                              >  1:348906/1‑251 (MQ=255)                                                                                                                                    gCCCGACCTACTGGAACAACGCGAAAACCG‑TTATTAACCAGGTAACC‑TATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACC‑GCTACC‑GTAGTGGTGAAATCGACAT‑GACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTG‑CAC‑TT‑ACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATgtgcgtgtgcgt                                                                                                             <  1:282015/251‑1 (MQ=255)                                                                                                                                       cacccctacgggaaaactcataaaCCGGTTATAAACCAGGTAACC‑TATGTGACAATTTCTGCTAAAGTTACTGATGTCAGCC‑GCATCG‑GTTGTGGGAAAAACGACATAGA‑GGATAAACGCTTGCCGAGCGATTTGTTCCAAAATCTTAAAAAATAGCTCCCGTTCGAAGGCCACGTTGATCCATTCCTGTG‑CAC‑TT‑ACTTTTACGAAATGAACGACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTAc                                                                                                           <  2:723228/230‑1 (MQ=255)                                                                                                                                          cctcatGGAACAACGCAAAACCCG‑TTATTACCCAGGTACCT‑TATTTGCCTTTTGCTTCTGAAGTTAACGTTGTCAACC‑GCTCCC‑GGAGTGGTGAAATCGACAT‑GAGGGATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGTGATCCCGGACGAAGTCCCCGTTGATCCATTCC‑GTGGCAC‑TT‑AGTATTACGAAATTAACAGCCAAAAACCGCCATTCAACGATTTGCGTGTGCGTACCGCa                                                                                                       <  2:445288/246‑1 (MQ=255)                                                                                                                                          ccTACTGGAACAACGCGAAAACCG‑TTATTAACCAGGTAACC‑TATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACC‑GCTACC‑GTAGTGGTGAAATCGACAT‑GACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGCAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTG‑CAC‑TT‑ACTATTACGAAATTAAAAACCAGAAACCGCCATTCAAAGATGTGCGAGTGCGTACCGc                                                                                                        >  1:107353/1‑250 (MQ=255)                                                                                                                                            tACTGGAACAACGCGAAAACCG‑TTATTAACCAGGTAACC‑TATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACC‑GCTACC‑GTAGTGGTGAAATCGACAT‑GACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTG‑CAC‑TT‑ACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACt                                                                                                     >  1:805309/1‑251 (MQ=255)                                                                                                                                             acggtaacaccccaaaaaCCG‑TTATTAACAAGGTAACC‑TATTTGCCTATTGCTTCTGAATTTCCCGATGTCAACC‑GCTACC‑GTAGTGGTGAAATCGACAT‑GACGTATAACAGCATGCCGATCGAATTGTTCCAGATGCTGAAAAAAGAGTTCCCGGACGAAGTCCACGTTGATCCATACCTGTG‑CAC‑TT‑GCTATTACGAATTTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTTCGTACCGCAc                                                                                                      <  2:844099/235‑1 (MQ=255)                                                                                                                                               tGGAACAACGCGAAAACCG‑TTATTAACCAGGTAACC‑TATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACC‑GCTACC‑GTAGTGGTGAAATCGACAT‑GACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTG‑CAC‑TT‑ACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGAACCGCACTGa                                                                                                   >  2:979789/1‑250 (MQ=255)                                                                                                                                                  aacaacGCGAAAACCG‑TTATTAACCAGGTAACC‑TATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACC‑GCTACC‑GTAGTGGTGAAATCGACAT‑GACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTG‑CAC‑TT‑ACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACt                                                                                               >  1:254630/1‑251 (MQ=255)                                                                                                                                                  aacaacGCGAAAACCG‑TTATTAACCAGGTAACC‑TATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACC‑GCTACC‑GTAGTGGTGAAATCGACAT‑GACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTG‑CAC‑TT‑ACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACt                                                                                               >  2:306933/1‑251 (MQ=255)                                                                                                                                                      acGCGAAAACCG‑TTATTAACCAGGTAACC‑TATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACC‑GCTACC‑GTAGTGGTGAAATCGACAT‑GACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTG‑CAC‑TT‑ACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGt                                                                                           >  1:514725/1‑251 (MQ=255)                                                                                                                                                         cgAAAACGG‑TAATTAACCAGGTACCT‑TAGTTGCCTATGGCTGTTGAATTTGCCGATG‑CAACCCTCTACCTGT‑GTGGTGAAATCGACAG‑GACGTAAAACAGCATGCCGATCGAATTGATCATGACACTGAAAAAAGAGACCCCGGCCGAAGTACACGTTGATCCATCCCGGTT‑CTC‑TT‑ACTATTCCGAAATTACCAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATg                                                                                        <  2:109363/251‑1 (MQ=255)                                                                                                                                                          gAAAACCG‑TTATTAACCAGGTAACC‑TATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACC‑GCTACC‑GTAGTGGTGAAATCGACAT‑GACGTATAACAGCATGCCGATAGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTG‑CAC‑TT‑ACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGAATgg                                                                                       >  2:475622/1‑251 (MQ=255)                                                                                                                                                          gAAAACCG‑TTATTAACCAGGTAACC‑TATTAGCATATTGCTTCTGTAGTTACCGATGTCAACC‑GCTACC‑GTAGTGGTCAAATCGACAT‑GACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTG‑CCC‑TTCAC‑ATTACGAAATTAACAACCAGAAACCGCCATTAAACGATGTGCGTGTGCGTACCGCCCTGCAACTAGGTATgg                                                                                       >  2:164341/1‑251 (MQ=255)                                                                                                                                                           aaaaCCG‑TTAGAAACCATGTAACC‑GATTTGCCTATTGTTTCTGAAGTTACCAATGTCAACC‑CTTACC‑GTAGGGGTGAAACGGACAT‑GACGAATAACACAATGCCGAGAGATTTGTTCCAGAAGCTGAAAAAAGACATCCCGGACGAAGTTCACGTTGACCAATCCCTGTG‑CAC‑TT‑ACTATTCCAAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATggt                                                                                      <  2:713108/251‑2 (MQ=255)                                                                                                                                                            aaatcctttattaATCAGGTAACC‑TATTGGCCTATTTCGTCTGAAGTAACCGATGACACCC‑GCTACC‑GTAGTTGTGAACACGCCAT‑GACGTTTAACCGCATGCCGATCGAATTGGTCAAGAGGCTTAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCTATCCCGGTC‑CTC‑TT‑GCTTTTGCGAATTTAACATCAAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGa                                                                                      <  2:609491/244‑1 (MQ=255)                                                                                                                                                                               tAACC‑TATTTGCCTATTGATTCTGAAGTTACCGATGTCAACC‑GCTACC‑GTAGTGGTGAAATCGACAT‑GACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTG‑CAC‑TT‑ACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGAAACGCGATATCATTGTtaat                                                                   >  2:826257/1‑251 (MQ=255)                                                                                                                                                                                aaCC‑TATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACC‑GCTACC‑GTAGTGGTGAAATCGACAT‑GACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTG‑CAC‑TT‑ACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTtaat                                                                   >  1:88036/1‑250 (MQ=255)                                                                                                                                                                                     tATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACC‑GCTACC‑GTAGTGGTGAAATCGACAT‑GACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTG‑CAC‑TT‑ACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAgg                                                              >  1:797351/1‑250 (MQ=255)                                                                                                                                                                                      aTTTGCCTATTGCTTCTGAAGTTACCGATGTCAACC‑GCTACC‑GTAGTGGTGAAATCGACAT‑GACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTG‑CAC‑TT‑ACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTg                                                             >  1:432673/1‑251 (MQ=255)                                                                                                                                                                                       tttcccTATTGCTTCTGAAGTTACCGAGTTCAACC‑TCTACC‑GTGGTGGTAAAATCGACAT‑GCCGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTGGATCCATTCCTGTG‑CAC‑TT‑ACTATTACGAAATTAACAACCATAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTg                                                             <  1:213417/246‑1 (MQ=255)                                                                                                                                                                                         tGCCTATTGCTTCTGAAGTTACCGATGTCAACC‑GCTACC‑GTAGTGGTGAAATCGACAT‑GACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCAt                                                                                                                                                                                 >  1:834289/1‑135 (MQ=255)                                                                                                                                                                                         tGCCTATTGCTTCTGAAGTTACCGATGGCAACC‑GCTACC‑GTAGTGGTGAAATCGACAT‑GACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCAt                                                                                                                                                                                 <  2:834289/135‑1 (MQ=255)                                                                                                                                                                                          gCCTATTGCTTCTGAAGTTACCAATGTCAAAC‑GCTACC‑GTAGTGGTGAAATCGACCT‑GACGTATAACAGCATGCCGATCGAATTGTTCCAGCAGCTGAAAAACGAGATCCCGGACGAAGTTCACGTTGCTACATACCTGTG‑CAC‑TT‑ACTATTACGAAATTACCAACAAGAAACCGCCATTCACCGATGTGAGTGTGCGTACCGAACTGAAACTAGGGATGGACCGCGATATCATTGTtaataacgtaaaa                                                          >  2:82596/1‑243 (MQ=255)                                                                                                                                                                                           ccTATTGCTTCTGAAGTTACCGATGTCAACC‑GCTACC‑GTAGTGGTGAAATCGACAT‑GACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTG‑CAC‑TT‑ACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAgc                                                        <  1:306933/251‑1 (MQ=255)                                                                                                                                                                                                tGCTTCTGAAGTTACCGATGTCAACC‑GCTACC‑GTAGTGGTGAAATCGACAT‑GACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCACGGACGAAGTTCACGTTGATCCATCCCTGTG‑CAC‑TT‑ACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGCGCGTGTGCGTACCGCACTGCAACTAGGTATGGACAGCGATATCATTGTTACTAAAGTGaaattgcagg                                                   >  2:224080/1‑244 (MQ=255)                                                                                                                                                                                                  cTTCTGAAGTTACCGATGTCAACC‑GCTACC‑GTAGTGGTGAAATCGACAT‑GACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTG‑CAC‑TT‑ACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGc                                                 >  1:266465/1‑251 (MQ=255)                                                                                                                                                                                                   ttCTGAAGTTACCGATGTCAACC‑GCTACC‑GTAGTGGTGAAATCGACAT‑GACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTG‑CAC‑TT‑ACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCCGGGc                                                 >  2:512456/1‑250 (MQ=255)                                                                                                                                                                                                    tcggAAGTTACCGATGTCAACC‑GCTACC‑GGAGTGGTGAAACCGACAT‑GACGTAAAACAGCATGCCGATCGAATTGTCCCAGAAGCTGAAAAAAGAGATCCCGGACAAAGTTCACGTTGATCCATACCTGTG‑CAC‑TT‑CCTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCa                                                <  1:475622/247‑1 (MQ=255)                                                                                                                                                                                                     cTGAAGTTACCGATGTCAACC‑GCTACC‑GTAGTGGTGAAATCGACAT‑GACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTG‑CAC‑TT‑ACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAAc                                              >  1:38015/1‑251 (MQ=255)                                                                                                                                                                                                       gAAGTTACCGATTTCAACC‑GCTACC‑GTAGTGGTGAAATCGACAT‑GACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTG‑CAC‑TT‑ACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACa                                             <  1:300215/250‑1 (MQ=255)                                                                                                                                                                                                       gAAGTTACCGATGTCAACC‑GCTACC‑GTAGGGGTGAAATCGAAAT‑GACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGACCCCGGACGAAGTTCACGTTGATCCATCCCTGTG‑CAC‑TT‑ACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACAt                                            <  1:389762/251‑1 (MQ=255)                                                                                                                                                                                                          gTTACCGATGTCAACC‑GCTACC‑GTAGTGGTGAAATCGACAT‑GACGTATAACAGCCTGCCGATCGAATTGTTCCAGAAGCTGAAAAATGAGATCCCGGACGCAGTTCACGTTGATCCATCCCTGTG‑CAC‑TT‑ACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGCAACTAGGTATAGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGcc                                         >  2:102793/1‑251 (MQ=255)                                                                                                                                                                                                            tACCGATGTCAACC‑GCTACC‑GTAGTGGTGAAATCGACAT‑GACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAGGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTG‑CAC‑TT‑ACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCg                                       >  1:737513/1‑251 (MQ=255)                                                                                                                                                                                                            tACCGATGTCAACC‑GCTACC‑GTAGTGGTGAAATCGACAT‑GACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTG‑CAC‑TT‑ACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGcccc                                       >  2:859623/1‑250 (MQ=255)                                                                                                                                                                                                            tACCGATGTCAACC‑GCTACC‑GTAGGGGTAAAAGCAACAT‑GACGAATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGACCCATCCCTGTG‑CAC‑TT‑ACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGccc                                        <  2:360829/250‑1 (MQ=255)                                                                                                                                                                                                               cGATGTCAACC‑GCTACC‑GTAGTGGTGAAATCGACAT‑GACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTG‑CAC‑TT‑ACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCt                                    >  1:421441/1‑251 (MQ=255)                                                                                                                                                                                                                           gCTACC‑GTAGTGGTGAAATCGACAT‑GACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTG‑CAC‑TT‑ACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGTTACACt                         >  1:708443/1‑251 (MQ=255)                                                                                                                                                                                                                           gCTAAC‑GTAGTGGTTAAATCGACAT‑GACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTG‑CAC‑TT‑ACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGTTACACt                         >  2:160728/1‑251 (MQ=255)                                                                                                                                                                                                                              aCC‑GTAGTGGTGAAATCGACAT‑GACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTG‑CAC‑TT‑ACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGTTACACTcc                       >  1:118070/1‑250 (MQ=255)                                                                                                                                                                                                                                c‑GTAGTGGTGAAATCGACAT‑GACGGATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTG‑CAC‑TT‑ACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACTTGCCCGCCTATGGTTACACTCCAcc                    >  1:659812/1‑251 (MQ=255)                                                                                                                                                                                                                                     gtggtgAAATCGACAT‑GACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAACAAAGAGATCCCGGACGAAGTTCACGTTGATCAATACCTGTG‑CAC‑TT‑ACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGTTACACTCCACCGtat                >  2:614330/1‑251 (MQ=255)                                                                                                                                                                                                                                             aTCGACAT‑GACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTG‑CAC‑TT‑ACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGTTACCCTCCCCCGTATACTGATgg        >  2:964150/1‑251 (MQ=255)                                                                                                                                                                                                                                                gACAT‑GACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTG‑CAC‑TT‑ACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGACTATGGTTACACTCCACCGTATACTGATgacg      >  2:223741/1‑247 (MQ=255)                                                                                                                                                                                                                                                    t‑GACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTG‑CAC‑TT‑ACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGGAACATGCCAGCCTATGGTTACACTCCACCGAATACTGATGGAGCaaa  >  2:331611/1‑250 (MQ=255)                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                        AACTTCACCGGTGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCG‑TTATTAACCAGGTAACC‑TATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACC‑GCTACC‑GTAGTGGTGAAATCGACAT‑GACGAATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTG‑CAC‑TT‑ACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGTTACACTCCACCGTATACTGATGGCGCAAA  >  NC_000913/1301748‑1302236

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑