Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	65,594	A→C	F63V (TTT→GTT)	polB ←	DNA polymerase II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	65,594	0	A	C	92.6%	205.8 / ‑2.5	81	F63V (TTT→GTT)	polB	DNA polymerase II
Reads supporting (aligned to +/- strand):  ref base A (4/2);  new base C (23/52);  total (27/54)
Fisher's exact test for biased strand distribution p-value = 9.17e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.34e-01

TCGGGATGCGGTTTCAGACGGGCATTAACGATAGTGCCATTGTGCATATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCTCCATCAGATAGCGTTCTGGCGGACGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATA‑CACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAAA‑GCCTTGTTCA‑CCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGG‑AATAAACGCCACGGACTCTTGCGGTGCAAGCGTAAC‑CTGCAACGGCCCGTTGTCCGTCGCCAGCCAGAAGGAGACTTCTGT‑CCCTTGCGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGAAAATCCATC‑AAAAAACCAGGCTTGAGTATAGCCTGGTTTCGTTTGATT  >  NC_000913/65347‑65833                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                      gcGGGATGCGGTTCCAGTCGGGCATTAACGATAGCGCCATTGTCCATATCCCCCTCGACCCACACCGGGGAGGTGATAAACCGCTCCATCAGATAGCGTTCTGGCGGGCGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATGGGCGATGGGCGCGACAGTAAAGGCCATA‑CACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAAC‑Gcc                                                                                                                                                                                                                                                   <  2:838653/250‑1 (MQ=255)  cGGGATGCGGTTGCAGACGGGCATTAACGATAGCGCCATTGTGCATATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCTCCATCAGATAGCGTTCTGGCGGGCGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCAGCAATTGGCGATGGGCGCGACAGTAAAGGCCATA‑CACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAAC‑Gcc                                                                                                                                                                                                                                                   <  1:153063/250‑1 (MQ=255)    ggATGCGGTTTCAGACGGGCATTAACGATAGCGCCATTGTGCATATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCTCCATCAGATAGCGTTCTGGCGGGCGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTGTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATA‑CACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAAC‑GCCTTg                                                                                                                                                                                                                                                <  1:629644/251‑1 (MQ=255)        gCGGTTTCAGTCGGGCATTAACGATAGCGCCTTTGTTCATATCACCCTCGTCCCACACCGGTGAGGTGATAAACCGCTCCATCAGATAGCGTTCTGGCGGGCGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGAGGGGCGCGACAGTAAAGGCCATA‑CACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAAC‑GCCgtgtaca                                                                                                                                                                                                                                            <  1:34572/251‑4 (MQ=255)           gTTTCAGACGGGCATTAACGATAGCGCCATTGTGCATATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCTCCATCAGATAGCGTTCTGGCGGGCGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATA‑ACCCGGCTGGCGGAGGAAATCCTTTAACGCCCACGGTGTCAGGCGACC‑GCCTTGTTCA‑ccc                                                                                                                                                                                                                                        >  1:374577/1‑251 (MQ=255)              tCAGACGGGCATTAACGATAGCGCCATTGTGCATATCACCCTCTACCCACACCGGTGAGGTGATAAACCGCTCCAGCAGATATCGTTCTGGCGGGCGCACATCGGGCTCGTAGACGGTAACGCCACCTTCACGCGGGCGCTTTTCGTAATTCATCAATTGGAGTTGGGCGCTACAGTAAAGGCCATA‑CAGCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAAC‑GCCTTGTTCA‑CCCTg                                                                                                                                                                                                                                      <  1:608976/250‑1 (MQ=255)                aGACGGGCATTAACGATAGTGCCATTGTGCATATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCTCCATCAGATAGCGTTCTGGCGGACGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGCCAGTAAAGGCCATA‑CACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCCGGGTCAGGCGAAAAGCCT‑GTTCA‑CCCTGCa                                                                                                                                                                                                                                    >  2:456249/1‑250 (MQ=255)                aGACGGGCATTAACGATAGCGCCATTGTGCATATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCTCCATCAGATAGCGTTCTGGCGGGCGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATA‑CACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAAC‑GCCTTGTTCA‑CCCTGCaa                                                                                                                                                                                                                                   <  1:889389/251‑1 (MQ=255)                  aCGGGCATTAACGATAGCGCCATTGTGCATATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCTCCATCAGATAGCGTTCTGGCGGGCGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATA‑CACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAAC‑GCCTTGTTCA‑CCCTGCaaaa                                                                                                                                                                                                                                 <  1:948984/251‑1 (MQ=255)                      gCATTAACGATAGCGCCATTGTGCATATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCTCCATCAGATAGCGTTCTGGCGGGCGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATA‑CACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAAC‑GCCTTGTTCA‑CCCTGCAAAATATg                                                                                                                                                                                                                             <  1:570391/251‑1 (MQ=255)                          tAACGATAGCGCCATTGTGCATATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCTCCATCAGATAGCGTTCTGGCGGGCGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATA‑CACCGGCTGGCGGTGAAAATCCTTTTACGCCAGCGGGGTCAGGCGAAC‑GCCTTTTTCA‑CCCTGCCAAATATGCTgg                                                                                                                                                                                                                         >  1:201596/1‑250 (MQ=255)                          tAACGATAGCGCCATTGTGCATATCACCCTAGACCCACACCGGTGAGGTGATAAACCGCTCCATCAGATAGCGTTCTGGCGGGCGCACATCGGCCTCGTAGACGGTAACGCCACCTGCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATA‑CACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAAC‑GCCTTGTTCA‑CCCTGCAAAATATGCTg                                                                                                                                                                                                                          <  2:806150/250‑1 (MQ=255)                             cGATAGCGCCATTGTGCATATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCTCCATCAGATAGCGTTCTGGCGGGCGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATA‑CACCGGCTGGCGGTGAAAATCCTTTACCGCCAACTGTGTCAAGCGAAA‑GGCTTGTTCA‑CCCTGCCAAATATGCTGAgcg                                                                                                                                                                                                                      >  1:989645/1‑251 (MQ=255)                             cGATAGCGCCATTGTGCATATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCTCCATCAGATAGCGTTCTGGCGGGCGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATA‑CACCGGCTGGCGGTGAAAATCCTTTAAAGCCAGCGGTGTCAGGCGACC‑GCCTTGTTCA‑CCCCGCAAAATATGCTGAgcg                                                                                                                                                                                                                      >  1:89592/1‑251 (MQ=255)                             cGATAGCGCCATTGTGCATATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCTCCATCAGATAGCGTTATGGCGGGCGCACATCGGCCTCGTAGACGGTAACGCCACCTTCCCGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATA‑CACCGGCTGGCGGTGAAAAACCTTTAAAGCCAACGGTGTCAGGCGAAC‑GACTTGTTCA‑CCCTGCAAAATATgactag                                                                                                                                                                                                                        >  1:536217/1‑244 (MQ=255)                                tagcgCCATTGTGCATATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCTCCATCAGATAGCGTTCTGGCGGGCGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATA‑CACCGGCTGGCGGTGGAAATCCTTTTACGCCAGCGGTGTCAGGCGAAC‑GCCTTGTTCC‑CCCTGCAAAATATGCTGAGCGCgg                                                                                                                                                                                                                   >  1:770755/5‑251 (MQ=255)                                 agcgCCATTGTGCATATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCTCCATCAGATAGCGTTCTGGCGGGCGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATA‑CACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAAC‑GCCTTGTTCA‑CCCTGCAAAATATGCTGAGCGCgg                                                                                                                                                                                                                   <  1:935836/247‑1 (MQ=255)                                    gCCATTGTGCATATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCTCCATCAGATAGCGTTCTGGCGGGCGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATA‑CACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAAC‑GCCTTGTTCA‑CCCTGCAAAATATGCTGAGCGCGGGGaa                                                                                                                                                                                                               <  1:295390/251‑1 (MQ=255)                                     ccATTGTGCATATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCTCCATCAGATAGCGTTCTGGCGGGCGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGGAAAGGCCATA‑CACCGGCTGGCGGGGAAAATCCTTTAACGCCAGCGGGGTCAGGCGAAC‑GCCTTTTTCAACCCT‑CAAAAAATGCTGAGCGCGGGGagc                                                                                                                                                                                                              >  1:781459/1‑249 (MQ=255)                                          gtgCATATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCTCCATCAGATAGCGTTCTGGCGGGCGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATA‑CACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAAC‑GCCTTGTTCA‑CCCTGCAAAATATGCTGAGCGCGGGGAACCTGAt                                                                                                                                                                                                         <  1:370960/251‑1 (MQ=255)                                                   aCCCTCGACCCACACCGGTGAGGTGATAAACCGCTCCATCAGATAGCGTTCTGGCGGGCGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATA‑CACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGGGTCAGGCGAAA‑GCCTTGTTCA‑CCCCGCAAAATATGCTGAGCGCGGG‑AACCTGAACGGCgggga                                                                                                                                                                                                >  1:466121/1‑248 (MQ=255)                                                    cccTCGACCCACACCGGTGAGGTGATAAACCGCTCCATCAGATAGCGTTCTGGCGGGCGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATA‑CACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAAA‑GCCTTGTTCA‑CCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCgggga                                                                                                                                                                                                >  1:411690/1‑248 (MQ=255)                                                               cacCGGTGAGGTGATAAACCGCTCCATCAGATAGCGTTCTGGCGGGCGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATA‑CACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAAC‑GCCTTGTTCA‑CCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGG‑AATAAACGCCACgg                                                                                                                                                                                   <  1:298302/251‑1 (MQ=255)                                                                 cctgggatgtgaTAACCCGGTCCATCAGATAGCGTACTGGCGGGCGCACAGCGGCTTCGTAGACGGTAACGCCACCTTCACGCAAGCGCTTGTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATA‑CACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAAC‑GCCTTGTTCA‑CCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGG‑AATAAACGCCACGGAc                                                                                                                                                                                 <  2:305255/243‑1 (MQ=255)                                                                 ccGGTGAGGTGATAAACCGCTCCATCAGATAGCGTTCTGGCGGGCGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATA‑CACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGGAC‑GCCTTGTTCA‑CCCTGCAAAATATGCTGGGCGCGGGGAACCTGATCGGCggggaaaaaacccccgg                                                                                                                                                                                    >  1:788308/1‑235 (MQ=255)                                                                         gtgaTAAACCGCTCCATCAGATAGCGTTATGGCGGGCGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGGAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATA‑CACCGGCTGGCGGGGAAAATCCTTTAACGCCCGCGGGGTCAGGCGAAC‑GCCTTGTTCA‑CCCTGCAAAATATGCTGAGCGAGGGGAACCTGGACGGCGGG‑GAAAAAAGCCACgggccctttcgg                                                                                                                                                                         >  1:709655/1‑241 (MQ=255)                                                                          tgaTAAACCGCTCCATCAGATAGCGTTCTGGCGGGCGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATA‑CACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAAC‑GCCTTGTTCA‑CCCTGCAAAATATGCTGAGCGCGGGGAACCTGGTCGGCGGG‑GAAAAAAGCCCCGGGCTCtttgggg                                                                                                                                                                        >  1:498122/1‑246 (MQ=255)                                                                           gaaaaaCCGCTCCATCAGATATCGTTCTGTCTGGCGCCCCTCGTCCGCGTAGACGGGTACGCCACCTTCACGCAGGCGTTTTTCTTAATTCATCAATTGGCGATGGGCGTGACAGTAAAGGCCATA‑CACCGGCTGGCGGTGAAAAGCCTTTAACGCCAGCGGTGTCAGGCGAAC‑GCCTTGTTCA‑CCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGTCGGG‑AATAAActcctctgcatctttcggtt                                                                                                                                                                       <  2:707650/248‑20 (MQ=255)                                                                              aaaCCGCTCCATCAGATAGCTGTCTGGCGGGCGCAAAGCGGCCTCGTAGACGGTAACGCCCCCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATA‑CACCTGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAAC‑GCCTTGTTCA‑CCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGG‑AATAAACGCCACGGACTCTTGCGGTGCa                                                                                                                                                                     <  2:674666/250‑1 (MQ=255)                                                                                         ccAGATAGCGTTCTGGCGGGCGCCCATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATA‑CACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAAC‑GCCTTGTTCA‑CCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGG‑AATAAACGCCACGGACTCTTGCGGTGCAAGCGTAAC‑CTGc                                                                                                                                                        <  1:543138/250‑1 (MQ=255)                                                                                            gATAGCGTTCTGGCGGGCGCACATCGGCCTCGAAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATA‑CACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAAC‑GCCTTGTTCA‑CCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGG‑AATAAACGCCACGGACTCTTGCGGTGCAAGCGTAAC‑CTGCAAc                                                                                                                                                     <  1:65218/251‑1 (MQ=255)                                                                                            gATAGCGTACTGGCGGGCGCACATCGGCCCCGTAGACGGTAACGCCCCCTTCACGCAGGCGATTTGCTTAATTCATCAATTGGCGATGGGCGCGACATTAAAGGCCATA‑CACCGGCTGGCGGTGAAAATCCGTTAACTCCAGCGGTGTCAGGCGAAC‑GCCTTGTTCT‑CCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGCG‑AATAAACGCCTCGGACTCTTGCGGTGCAAGCGTACC‑CTGCAAc                                                                                                                                                     <  2:360771/251‑1 (MQ=255)                                                                                             atagccgtCTGGCGGGCGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATA‑CACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAAC‑GCCTTGTTCA‑CCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGG‑AATAAACGCCACGGACTCTTGCGGTGCAAGCGTAAC‑CTGCAACg                                                                                                                                                    <  2:358069/244‑1 (MQ=255)                                                                                                  gTTCTGGCGGGCGCACTTCGGCCGTGTAGACGGTAACGCCCCCTTCACGCAGGCGTTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATA‑CACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAAC‑GCCTTGTTCA‑CCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGG‑AATAAACGCCACGGACTCTTGCTGTGCAAGCGTAAC‑CTGCAACGGcacg                                                                                                                                               <  2:283723/251‑4 (MQ=255)                                                                                                       ggcgggcgCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATA‑CACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAAC‑GCCTTGTTCA‑CCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGG‑AATAAACGCCACGGACTCTTGCGGTGCAAGCGTAAC‑CTGCAACGGCCCGtt                                                                                                                                             <  1:704882/248‑1 (MQ=255)                                                                                                       ggcgggcgCACATCGCCCTCGTAGACGTTAACGCCACCTTCACTCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATT‑CACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCTAAC‑GCCTTGTTCT‑CCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGG‑AATAAACGCCACGGACTCTTGCGGTGCAAGCGTAAC‑CTGCAACGGCCCTTTGt                                                                                                                                           <  1:431434/250‑1 (MQ=255)                                                                                                       ggCGGTCGCACTGCGGCCTCGTTGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGACGGGCGCGACAGTAAAGGCCAGA‑CACCGGCGTGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAAC‑GCCTTGTTCA‑CCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGG‑AATAAACGCCACGGACTCTTGCGGTGCAAGCGTAAC‑CTGCACCGGCCCGtt                                                                                                                                             <  2:582609/248‑1 (MQ=255)                                                                                                        gCGGGCGCACATCGGCCGCGTAGACGGTAACGCCACCGGCACGCAGGCGCTTTTCGTAATTCAGCAATTGGCGATGGGCGCGACAGTAAAGGCCATA‑CACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAAC‑GCCTTGTTCA‑CCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGG‑AATAAACGCCACGGACTCTTGCGGTGCAAGCGTAAC‑CTGCAACGGCCCGTTGTcc                                                                                                                                         <  1:955311/251‑1 (MQ=255)                                                                                                             cGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGTGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATA‑CACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAAC‑GCCTTGTTCA‑CCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGG‑AATAAACGCCCCGGGCTCTTGCGGTGCAAGCGTAAA‑CCGCAACGGccccgttgccctcgc                                                                                                                                    >  1:929308/1‑239 (MQ=255)                                                                                                               caaaTCGGCCTCGTAGAAGGTAACGCCACCTTCACGCAGGCGCGTGTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATA‑CACCGGCTGGCGGTGAAAAGCCTTTAACGCCAGCGGTGTCAGGCGAAC‑GCCTTGTTCA‑CCCTGCAAAATATGCTGAGCGCGGGGAACCTGAGCGGCGGG‑AATAAACGCCACGGACTCTTGCGGTGCAAGCGTAAC‑CTGCAACGGCCCGTTGTCCGTCgcca                                                                                                                                  <  2:201596/248‑1 (MQ=255)                                                                                                                    cGGCCTTGTAGACGGGATCGCCACCTACACGCAGGCGTGTTTCGAAAGGCATCATATGTCGATGGGCGCGACAGTAAAGGCCATA‑CTCTGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGCCAGGCGAAC‑GCCTTGTTCA‑CCCTGCAAAATATGCTGAGCGCGTGGACCCTGACCGTCGGG‑ACTAAACGCCGCGGACTCTTGCGGTGCCAGCGTAAT‑CTGCAACGGCCCGTTGTCCGTCGccatccag                                                                                                                             <  2:108069/251‑1 (MQ=255)                                                                                                                      gCCTCGTAGACGGTAACGCCACCTGCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATA‑CACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAAC‑GCCTTGTTCA‑CCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGG‑AATAAACGCCACGGACTCTTGCGGTGCAAGCGTAAC‑CTGCAACGGCCCGTTGTCCGTCGCCAGCCAGaa                                                                                                                           <  1:933668/251‑1 (MQ=255)                                                                                                                          cGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATA‑CACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAAC‑GCCTTGTTCA‑CCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGG‑AATAAACGCCACGGACTCTTGCGGTGCAAGCGTAAC‑CTGCAACGGCCCGTTGTCCGTCGCCAGCCAGAAGgag                                                                                                                       <  1:570352/251‑1 (MQ=255)                                                                                                                                    aaCGCCACCTTCACGCAGGCTCTTGTCGTAATTCATCAATGTGCGATGGGCGCGACAGTAAAGGCCATA‑CACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAAC‑GCCTTGTTCA‑CCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGG‑AATAAACGCCACGGACTCTTGCGGTGCAAGCGTAAC‑CTGCAACGGCCCGTTGTCCGTCGCCAGCCAGAAGTAGACTTCTGT‑cc                                                                                                            <  2:516656/251‑1 (MQ=255)                                                                                                                                    aaCGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATA‑CACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAAC‑GCCTTGTTCA‑CCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGG‑GATAACCGCCACGGGCTCTTGCGGTGCCATCGTTAA‑CTGCAACGGCCCCTTTTCCGTCGCCCGCCCGAAGGAGACTTCTTT‑cc                                                                                                            >  1:296285/1‑251 (MQ=255)                                                                                                                                     aCGCCACCTTCCCGCATGCGCTTGTCGTAATCCATCAATTGGCGAGGGGCGAGACAGTAAAGGCCATA‑CACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAAC‑GCCTTGTTCA‑CCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGG‑AATAAACGCCACGGACTCTTGCGGTGCAAGCGTAAC‑CTGCAACGGCCCGTTGTCCGTCGCCAGCCAGAAGGAGACTTCTGtacc                                                                                                            <  2:265431/251‑4 (MQ=255)                                                                                                                                       gCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATA‑CACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAAC‑GCCTTGTTCA‑CCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGG‑AATAAACGCCACGGACTCTTGCGGTGCAAGCGTAAC‑CTGCAACGGCCCGTTGTCCGTCGCCAGCCAGAAGGAGACTTCTGT‑CCCtt                                                                                                         <  1:193960/251‑1 (MQ=255)                                                                                                                                         cACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATA‑CACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAAC‑GCCTTGTTCA‑CCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGG‑GATAAAAGCCCCGGACTCTTGCGGGGCAAGCGTAAC‑CCGCAACGGCCCGGTGTCCGTCGCCAGCCAGAAAGAGACCTCTGT‑CCCCTGc                                                                                                       >  1:450588/1‑251 (MQ=255)                                                                                                                                          aCCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATA‑CACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAAC‑GCCTTGTTCA‑CCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGG‑GATAAACGCCACGGACCCCTGTGGGGGAACCGTAAA‑CCCCAACGGCCCGTTGTCCTGCGCCCGCCCGAAgggagcttttttccccttgg                                                                                                       >  2:166988/1‑233 (MQ=255)                                                                                                                                               cACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATA‑CACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAAC‑GCCTTGTTCA‑CCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGG‑AATAAACGCCACGGACTCTTGCGGTGCAAGCGTAAC‑CTGCAACGGCCCGTTGTCCGTCGCCAGCCAGAAGGAGACTTCTGT‑CCCTTGCGGGgtg                                                                                                 <  1:809202/251‑1 (MQ=255)                                                                                                                                                   cAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATA‑CACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAAC‑GCCTTGTTCA‑CCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGG‑GATAAAAGCCACGGGCTCTTGCGGGGCAAGGGTAAA‑CTGCAACGGCCCGTTGTCCGTTGCCAGCCAGAAGGAGACTTTCGT‑CCCTTTCGTGGTGTccc                                                                                             >  1:531845/1‑251 (MQ=255)                                                                                                                                                   cAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATA‑CACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAAC‑GCCTTGTTCA‑CCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGG‑AATAAAAGCCACGGACTCTTGCGGTGCAAGCGTAAA‑CTGCAACGGCCCGTTGTCCGCCGCCCGCCAGAAGGAGACTTCTGT‑GCCTTGCGGGGGGTccc                                                                                             >  1:859145/1‑251 (MQ=255)                                                                                                                                                   cAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATA‑CACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAAC‑GCCTTGTTCA‑CCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGG‑AATAAAAACCCCGGACCCTTGCGGTGCAAGCGGTAC‑CCACCACGGCCCGTTGTCCGTTGCCCACCCGAAGGAGACCTCCTT‑CCCTTGCGTGGTGTccc                                                                                             >  1:211266/1‑251 (MQ=255)                                                                                                                                                   cAGGCGCTTTGCGTAATTCAGCAATTGGCGATGTGTGCGACCGTAAAGGCCCTA‑CTGCGGCTGGCGGTGAAATTCCTTTAACGCCAGGGGTGTCAGGCGAGC‑GCCTTGTTCC‑ACCTGCAAAATCTGCTGAGCGCGGGGAACCTGATCGGCGCG‑AATAAACGCCCCGGACTCTTGCTGTGCAAGCGTTAC‑CTGCAACTGTCCGTGGTCCGTCGCCAGCCAGAAGGAGACTTCTGT‑CCCTTGCGGGGTGTccc                                                                                             <  2:250585/251‑1 (MQ=255)                                                                                                                                                      gcgcTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATA‑CACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAAC‑GCCTTGTTCA‑CCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGG‑AATAAACGCCACGGACTCTTGCGGTGCAAGCGTAAC‑CTGCAACGGCCCGTTGTCCGTCGCCAGCCAGAAGGAGACTTCTGT‑CCCTTGCGGGGTGTCCCGcc                                                                                          <  1:711461/251‑1 (MQ=255)                                                                                                                                                        gcTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATA‑CACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAAC‑GCCTTGTTCA‑CCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGG‑AATAAACGCCACGGACTCTTGCGGTGCAAGCGTAAC‑CTGAAACGGCCCGTTGTCCGTCGCCAGCCAGAAGGAGACTTCTGT‑CCCTTGCGGGGTGTCCCGCCAg                                                                                        <  1:88794/251‑1 (MQ=255)                                                                                                                                                        gcTTTGCGTAATGCAGCAATTGGCGATGGTCGCGACAGTAAAGGCCATA‑CACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGGCAGGCGAAC‑GCCTTGTTCA‑CCCGGCAAAATATGCTGATCGCGGGGAACCGGATCGGCGGG‑AATAAACGCCACGGACTCTTGCGGTGCAAGCGTAAC‑CTGCAACGGCCCGTTGTCCGTCGCCAGCCAGAAGGAGACTTCTGT‑CCCTTGCGGGGTGTCCCGCCAg                                                                                        <  2:691599/251‑1 (MQ=255)                                                                                                                                                          ttttCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATA‑CACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAAC‑GCCTTGTTCA‑CCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGGGAAAAAAC‑CCACGGACTCTTTCGGGGCAAGCGTAAC‑CTGCAACGGCCCGGTGTCCGTCGCCAGCCCGAAGGAGACTTCTGTTCCCTT‑CGGGGGGTCCCGCCAgtg                                                                                      >  1:238683/1‑251 (MQ=255)                                                                                                                                                             tCGTAGTTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATA‑CACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAAC‑GCTTTGTTCA‑CCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGG‑AATAAACGCCACGGACTCTTGCGGTGCAAGCGTAAC‑CTGCAACGCCCCGTTGCCCGTCGCCAGCCAGAAGGAGACTTCTGT‑CCCTTGCGGGGTGTCCCGCCAGTGTc                                                                                    <  1:93000/250‑1 (MQ=255)                                                                                                                                                               gTAATTAATCAATGGGCGAGGGGCGCGAAAGTAAAGGC‑ATAACACCGGCTGGCGGTGAAAACCCTTTAACGCCAGCGGGGTCAGGCGAAC‑GCCTTGTTCA‑CCCGGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGG‑AATAAACGCCACGGACTCTTGCGGTGCAAGCGAAAC‑CTGCAACGGCCCGTTGTCCGTCGCCAGCCAGAAGGAGACTTCTGT‑CCCTTGCGGGGTGTCCCGCCAGTGTCggg                                                                                 <  2:258590/251‑1 (MQ=255)                                                                                                                                                                         aaTTGGCGATGGGCGCGACAGTAAAGGCCATA‑CACCGGCTGGCGGTGAAAATCCTTTAAAGCCAGCAGTGTCAGGCGAAC‑GCCTTGTTCA‑CCCTGCAAAATATGCTGAACGCGGGGAACCTGATCGGCGGG‑AATAAACACCAAAGACTCTTTCGGGGCAAGCGGGACACT‑CAACTGCCCCTTGTCCGTCCCCAGACAGAAGGAGGCTTCTGT‑CCCCTGGGGGGGTTTCCGCCAGTGGCggggttagagaa                                                                        >  1:724049/1‑241 (MQ=255)                                                                                                                                                                               cGATGGGCGCGACAGTAAAGGCCATA‑CACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAAC‑GCCTTGTTCA‑CCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGG‑AATAAACGCCACGGGCTCTTGCGGTGCAAGCGTAAC‑CCGCAACGGCCCGTTGTCCGTCGCCAGCCCGAAGGAGACTTCTGT‑CCCTTGCGGGGGGTCCCCCCCGGGTCGGGGTAAGATAAAAcatgc                                                                 >  1:168448/1‑251 (MQ=255)                                                                                                                                                                                gATGGGCGCGACAGTAAAGGCCATA‑CACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAAC‑GCCTTGTTCA‑CCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGG‑AATAAAAGCCACGGGCTTTTGCGGTGGAAGGGGAAA‑CTGCAAAGGCCCGTTGTCCGTCGCCCGCCAGAAGGAAAATTTTGT‑CCCCTGCGGGGTGTGCCGCCCGTGTgggggttaaatgaaaaactgc                                                                >  1:785394/1‑234 (MQ=255)                                                                                                                                                                                gATGGGCGCGACAGTAAAGGCCATA‑CACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAAA‑GCCTTGTTCA‑CCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGG‑AATAAACGCCACGGActct                                                                                                                                                                              <  1:448113/143‑1 (MQ=255)                                                                                                                                                                                   gggCGCGACAGTAAAGGCCATA‑CACCGGCTGGCGGTGAAAACCCTTTAACGCCAGCGGTGTCAGGCGAAC‑GCCTTGTTCA‑CCCTGCAAAATATGCTGAGCGCGGGGAACCTGAGCGGCGGG‑AATAAACGCCACGGACTCTTGCGGTGCAAGCGTAAC‑CTGCAACGGCCCGTTGTCCGTCGCCAGCCAGAAGGAGACTTCTGT‑CCCTTGCGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAAACctgcctgc                                                             <  1:434371/251‑1 (MQ=255)                                                                                                                                                                                   gggCGCGACAGAAAAGGCCATC‑CCCCGGCTGGCGGTGAAAACCCTGTAACGCCGGCGGGGTCAGGCGAAC‑GCTTTGTTCA‑CCCTGCAAAATGGGCTGAGCGCGGGGACCTTGATCGGCGGG‑AAAAACCCCCACGGACTCTTGCGGTGCAAGCGTACC‑CTGCAACGGCCCTTTGTCCGTCGCCAGCCAGAAGGAGACTTCTGT‑CCCTTGCGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAAACctgcctgc                                                             <  2:983954/251‑1 (MQ=255)                                                                                                                                                                                                      aTA‑CACCGGTGGGCGGTGAAAATCCTTTAACGCCAGCGGTTGCAGGCGAAC‑GCCTTGTTCC‑CCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGG‑AAAAAACTCCCCGGACTCTGGCGTTGCAAGCTTAAC‑CTGCACGGGCCCGTTGTCCGCCGCCAGCCAGAAGGAGACTTCTGT‑CCCTTGCGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGTAATTCCATc                                          <  2:70983/251‑1 (MQ=255)                                                                                                                                                                                                       tA‑CACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAAC‑GCCTTGTTCA‑CCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGG‑AAAAAAAGCCACGGACTCTTGCGGGGCAAAGGGAAA‑CTGCAAAGGCCCGTTGTCCGTCGCCAGCCAGGAAGAAACATCTGT‑CCCTTGAGGGGTGTACCGCCCGAGTCGGGGTAAGGGAAAAAATGCCTGCGCCAcacaggaaatcccca                                          >  1:359155/1‑236 (MQ=255)                                                                                                                                                                                                         acgccGGCGGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAAC‑GCCTTGTTCA‑CCCTGCAAAATATGCTGAGCGCGGGGAACCTGACCGGCGGG‑AATAAACGCCACGGACTCTTGCGGTGCAAGCGTAAC‑CTGCAACGGCCCGTTGTCCGTCGCAAGCCAGAAGGAGACTTCTGT‑CCCTTGCGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGTAATTCCATC‑a                                        <  1:671047/247‑1 (MQ=255)                                                                                                                                                                                                                  ggCGGTGAAGATCCTTTAACGCCAGCGGTGTCAGGCGAAC‑GCCTTGTTCA‑CCCTGCAAAATATTCTGAGCGCGGGAAACCTGATCGGCGGG‑AATAAACGCCACGGACTCTTGCGGTGCAAGCGTAAC‑CTGCACCGGCCCGTTGTCCGTCGCCAGCCAGAAGGAGACTTCTGT‑CCCTTGCGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGTAATTCCATC‑AAAAAACCAgg                              <  1:787095/251‑1 (MQ=255)                                                                                                                                                                                                                  ggCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAAC‑GCCTTGTTCA‑CCCTGAAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGG‑AATAAACGCCCCGGACTCTTGCGGTGCAAGCGTAAC‑CTGCAACGGCCCGTTGTCCGTCGCCAGCCAGAAGGAGACTTCTGT‑CCCTTGCGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGTAATTCCATC‑AAAAAACCAgg                              <  1:163660/251‑1 (MQ=255)                                                                                                                                                                                                                  ggCGGTAAAAACCCTTTAACGCCGGGGGTCTCAGGCGACC‑GCCGTGGTCT‑CCCTACAAAATATACCGAGGGGGTGGAACCTGATCGGCGGG‑AAAAACCGCGACAAATACTTGTGGTGCAAGCGTATC‑CTGGCAGGGCCCTTCGGCCGTCGCCAGCCAAAAGGAGTCGCCATA‑CCCTTGCGGGGTGCCCCTCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGTAATTCCATC‑GAAAGACCAg                               <  2:481367/250‑1 (MQ=255)                                                                                                                                                                                                                    cGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAAC‑GCCTTGTTCA‑CCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGG‑AATAAAAGCCACGGACTCTTGCGGTGCAAGAGTAAA‑CTGCAACGGACCGTTGTCCGTCGCCCGACAGAAGGAGACTTCTGT‑ACCTTTCGGGGTGTCCCGCCAGTGTCGGGGTAAGATAAAACACGCCTACGCCACGCTGTAATTTCATCTAAAAAAcaagcc                             >  1:250905/1‑246 (MQ=255)                                                                                                                                                                                                                      cgccgctcttccgatgtacAGCGGGGCCAGGCGAAC‑GCCTTGTTCA‑CCCTCAAAAATATGCTGGGGGCGGGGAACCGGATCGGCGGG‑AAAAAACGCCACGGAGTTTTGCGGTGAAAGCGTAAC‑CTGCAACGGCCCGTTGTCCGTCGCCAGCCAGAAGGAGACTTCTGC‑CCCTTGGGGGGTGGCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGTAATTCCATC‑AAAAAACCAGGCTTg                          <  2:523402/233‑1 (MQ=255)                                                                                                                                                                                                                        gAAAACCCTTTAACGCCAGCGGTGTCAGGCGATC‑GCCTTGTTCA‑CCCTGCAAAATATGCTGAG‑ACCGGGACCCGGATCGGCGGG‑AATAACCCCCCCGTACTCGTGCGGGGCAAGCGTACC‑CGGCACCGGCCCGTTGTCCGTCGCCAGCCAGAAGGAGACTTCTGC‑CCCTTGCGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGTAAGTCCATC‑AAAAAACCAGGCTTGatt                       <  2:466121/251‑3 (MQ=255)                                                                                                                                                                                                                                  tAACGCCAGCGGTGTCAGGCGAAC‑CCCTGGTCCA‑CCCTGCAAAATATGCGGAGCGCGGGGAACCGGATCGGCGGG‑AATAAACGCCACGCACTCTTGCGGTGCAAGCGTAAC‑CTGCAGCGTCCAGTTGTCCGTCGCCATCCCGAAGGAGATTTCTGT‑CCCTGGCGGGGGGTCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGTAATTCCATC‑AAAAAACCAGGCTTGATTATAGCCTg               <  1:61839/250‑1 (MQ=255)                                                                                                                                                                                                                                   aaccccAGCTGTGGCAGGCGACC‑GCCTTGTTCT‑CCCTGCAAAATATGCTGAGCGCGGGGAACTGGATCGGCGGG‑AATAAACGCCACGGACTCTGGCGGTGCAAGCGTAAC‑CTGCAACGGCCCGTTGTCCGGCGCCAGCCAGAAGGAGCCTTCTGT‑CCCTTGCGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTTCGCCACGCTGTAATTCCATC‑AAAAAACCAGGCTTGATTATAGCCTGGt             <  1:554229/247‑1 (MQ=255)                                                                                                                                                                                                                                      gCCAGCGGTGTCAGGCGAAC‑GCCTTGTTCA‑CCCTGCAAAATATGCTGCGCGCGGGGATCCTGATCGGCGGG‑AATAAACGCCACGTACTCTTGCGGTGCAAGCTTATC‑CTGCAACGGCCCGTTGTCCGTCGCCAGCCAGAAGTAGACTTCTGT‑CCCTTGCGGGGTTTCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCCCGCTGTAATTCCATC‑AAAAAACCAGGCTTGATTATAGCCTGGTTTc          <  1:461832/251‑1 (MQ=255)                                                                                                                                                                                                                                        cAGCGGTGTCAGGCGAAC‑GCCTTGTTCA‑CCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGG‑AATAAACGCCACGGACTCTGGCGGTGCAAGCGTAAC‑CTGCAACGGCCCGTTGTCCGTCGCCAGCCATAAGGAGACTTCTGT‑CCCTTGCGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGTAATTCCATC‑AAAAAACCAGGCTTGATTATAGCCTGGTTTCGt        <  1:584931/251‑1 (MQ=255)                                                                                                                                                                                                                                             gtgtCAGGCGAAC‑GCCTTGTTCA‑CCCTGCAAAATATGCTGATCGCGGGGAACCTGATCGGCGGG‑AATAAAAGCCACGGACTCTTTCGGTGCAAACGTAAC‑CTGCAACGGCCCGTTGTCCGTCGCCAGCCCGAAAGAGAATTCTGT‑CCCTTGCGGGGGGTCCCCCCCGTGGCGGGTTAAGATAAAACCTCCCTGCGCCCCGCCGTAATTCCATC‑AAAAAAACAAGCTTGTGtataaactgggtttgttttat   >  1:841146/1‑234 (MQ=255)                                                                                                                                                                                                                                               gtCAGGCGAAA‑GCCTTTTTCA‑CCCGGCAAAAGATGCTGAGCGCGGGGAACCTGATCGGCGGG‑AAAAACCTCCACGGATTCTTGCGGTGCAAGCGTAAC‑CTGCAACGGCCCTTTGTCCGTCGCCAGCCAGAAGGATACTTCTGT‑CCCTTGCGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGTAATTCCATC‑AAAAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGAtt  <  2:91370/250‑1 (MQ=255)                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                      TCGGGATGCGGTTTCAGACGGGCATTAACGATAGTGCCATTGTGCATATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCTCCATCAGATAGCGTTCTGGCGGACGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATA‑CACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAAA‑GCCTTGTTCA‑CCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGG‑AATAAACGCCACGGACTCTTGCGGTGCAAGCGTAAC‑CTGCAACGGCCCGTTGTCCGTCGCCAGCCAGAAGGAGACTTCTGT‑CCCTTGCGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGAAAATCCATC‑AAAAAACCAGGCTTGAGTATAGCCTGGTTTCGTTTGATT  >  NC_000913/65347‑65833

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑