Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,189,980	A→G	T156T (ACT→ACC)	phoP ←	DNA‑binding transcriptional dual regulator PhoP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,189,980	0	A	G	100.0%	195.8 / NA	57	T156T (ACT→ACC)	phoP	DNA‑binding transcriptional dual regulator PhoP
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (35/22);  total (35/22)

CAGCGAGAGCGGGAAAAAAAGACGCAGTAATTTTTTCATCAGCGCAATTCGAACAGATAGCCCTGGCCGCGAACGGTGGTAATCACTTCTTGGGGATATTGTGCCTGAATTTTTTTGCGCAGACGTCCCATCAGTACATCAATGGTATGGCTTTCCCGCAGCTCCGCATCCGGATAGAGTTGGAGCATTAACGAATCTTTGCTGACCACTTTGCCATTATTGCGTATCAACGTTTCCATA‑ATAGTGTATTCGAACGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGCGAGAGAGATCAACCTGAAACGGGGGG‑AGCGAAATGACCTGTGAAGCCAGACCGCTATTACGCCGCATTAATGCCTGCATTCGCGCCATCACCTCTTCAATATGAAACGGTTTAGTCACATAATCATCAGCACCGGCACTTAATACTTCGACTTTGTCCTGCCAGCTTTCACGGGCGGTTAATA  >  NC_000913/1189738‑1190218                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                cAGCGAGAGCGGGAAAAAAAGACGCAGTAATTTTTTCATCAGCGCAATTCGAACAGATAGCCCTGGCCGCGAACGGTGGTAATCACTTCTTGGGGATATTGTGCCTGAATTTTGTTGCGCAGACGTCCCATCAGTACATCAATGGTATGGCTTTCCCGCAGCTCCGCATCCGGATAGAGTTGGAGCATTAACGAATCTTTGCTGACCACTTTGCCATTATTGCGTATCAACGTTTCCATA‑ATGGTGTATTc                                                                                                                                                                                                                                         <  1:681466/251‑1 (MQ=255)           gggAAAAAAAGACGCAGTGATTTTTTCATCAGCGCAATTCGAACAGAGAGCCCTGGCCGCGAACGGTGGTAATCACTTCTTGGGGATAGTGTGCCTGAATTTTTGTGCGCAGACGTCCCATCAGTACATCAATGGTATGGCTTTCCCGCAGCTCCGCATCCGAATAGAGTTGGGGCATTAACGAATCTTTGCTTACCACTTTGCCATTAGTGCGTATCAACGTTTCCATA‑AAGGTGTATTCGAACGCGGTc                                                                                                                                                                                                                               <  2:159330/251‑1 (MQ=255)              aaaaaaaGACGCAGTAATTTTTTCATCAGCGCAATTCGAACAGATAGCCCTGGCCGCGAACGGTGGTAATCACTTCTTGGGGATATTGTGCCTGAATTTTTTTGCGCAGACGTCCCATCAGTACATCAATGGTATGGCTTTCCCGCAGCTCCGCATCCGGATAGAGTTGGAGCATTAACGAATCTTTGCTGACCACTTTGCCATTATTGCGTATCAACGTTTCCATA‑ATGGTGTATTCGAACGCGGTCAGt                                                                                                                                                                                                                            <  1:734737/251‑1 (MQ=255)              aaaaaaaGACGCAGTAATTTTTTCATCAGCGCAATTCGAACAGATAGCCCTGGCCGCGAACGGTGGTAATCACTTCTTGGGGATATTGTGCCTGAATTTTTTTGCGCAGACGTCCCATCAGTACATCAATGGTATGGCTTTCCCGCAGCTCCGCATCCGGATAGAGTTGGAGCATTAACGAATCTTTGCTGACCACTTTGCCATTATTGCGTATCAACGTTTCCATA‑ATGGTGTATTCGAACGCGGTCAGt                                                                                                                                                                                                                            >  1:771450/1‑251 (MQ=255)                aaaaaGACGCAGTAATTTTTTCATCAGCGCAATTCGAACAGATAGCCCTGGCCGCGAACGGTGGTAATCACTTCTTGGGGATATTGTGCCTGAATTTTTTTGCGCAGACGTCCCATCAGTACATCAATGGTATGGCTTTCCCGCAGCTCCGCATCCGGATAGAGTTGGAGCATTAACGAATCTTTGCTGACCACTTTGCCATTATTGCGTATCAACGTTTCCATA‑ATGGTGTATTCGAACGCGGTCAGttt                                                                                                                                                                                                                          >  1:607596/1‑251 (MQ=255)                                tttttCATCAGCGCAATTCGAACAGATAGCCCTGGCCGCGAACGGTGGTAATCACTTCTTGGGGATATTGTGCCTGAATTTTTTTGCGCAGACGTCCCATCAGTACATCAATGGTATGGCTTTCCCGCAGCTCCGCATCCGGATAGAGTTGGAGCATTAACGAATCTTTGCTGACCACTTTGCCATTATTGCGTATCAACGTTTCCATA‑ATGGTGTATTCGAACGCGGTCAGTTTGATCACTTCGTCAtt                                                                                                                                                                                                           >  1:934294/1‑250 (MQ=255)                                  tttCATCACCGCAATTCGAACAGATAGCCCTTGCCGCGAACGGTGGTAATCACTTCTTGGTGATATTGTGCCTGAATTTTTTTGCGCAGACGTCCCATCAGTACATCAATGGTATGGCTTTCCCGCAGCTCCTCATCCGGATAGAGTTGTAGCATTAACGAATCTTTGCTGACCACTTTGCCATTATTGCGTATCAACGTTTCCATA‑ATGGTGTATTCGAACGCGGTCAGTTTGATCACTTCGTCATTaa                                                                                                                                                                                                         <  2:315197/250‑1 (MQ=255)                                      atcaGCGAAATTCGAACAGATATCCCAGGTCGCGACCGGTGGAAATAACTTCTTGGGGATATGGTGCCTGAATTGTTTTGCGCAGACGTCCCATCAGTACATCAATGGTATGGCTTCCCCGCAGCTCCTCATCCGGATAGAGTTGGAGAATTAACGACACTTGGCTGACCACTTTGACCTTATTGAGTATCAACGTTACC‑TACATGTTGTATTCGACCACGGTCGGTTTGATACCTTCGACATTAAt                                                                                                                                                                                                        >  2:27355/1‑247 (MQ=255)                                       tcaGCGCAATTCGAACAGATAGCCCTGGCCGCGAACGGTGGTAATCACTTCTTGGGGATATTGTGCCTGAATTTTTTTGCGCAGACGTCCCATCAGTACATCAATGGTATGGCTTTCCCGCAGCTCCGCATCCGGATAGAGTTGGAGCATTAACGAATCTTTGCTGACCACTTTGCCATTATTGCGTATCAACGTTTCCATA‑ATGGTGTATTCGAACGCGGTCAGTTTGATCACTTCGTCATTAATAGAt                                                                                                                                                                                                    >  1:828389/1‑250 (MQ=255)                                        caGCGCAATTCGAACAGATAGCCCTGGCCGCGAACGGTGGTAATCACTTCTTGGGGATATTGTGCCTGAATTTTTTTGCGCAGACGTCCCATCAGTACATCAATGGTATGGCTTTCCCGCAGCTCCGCATCCGGATAGAGTTGGAGCATTAACGAATCTTTGCTGACCACTTTGCCATTATTGCGTATCAACGTTTCCATA‑ATGGTGTATTCGAACGCGGTCAGTTTGATCACTTCGTCATTAATAGATaa                                                                                                                                                                                                  <  1:239344/251‑1 (MQ=255)                                        caGCGCAATTCGAACAGATAGCCCTGGCCGCGAACGGTGGTAATCACTTCTTGGGGATATTGTGCCTGAATTTTTTTGCGCAGACGTACCATCAGTACATCAATGGTATGGCTTTCCCGCAGCTCCGCATCCGGATAGAGTTGGAGCATTAACGAATCTTTGCTGACCACTTTGCCATTATTGCGTATCACCGTTTCCATA‑ATGGTGTCTTCGAACGCGGTCAGTTTGATCACTTCGTCATTATTAgcta                                                                                                                                                                                                   >  2:630181/1‑247 (MQ=255)                                               aTTCGAACAGATAGCCCTGGCCGCGAACGGTGGTAATCACTTCTTGGGGATATTGTGCCTGAATTTTTTTGCGCAGACGTCCCATCAGTACATCAATGGTATGGCTTTCCCGCAGCTCCGCATCCGGATAGAGTTGGAGCATTAACGAATCTTTGCTGACCACTTTGCCATTATTGCGTATCAACGTTTCCATA‑ATGGTGTATTCGAACGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACgg                                                                                                                                                                                           <  1:126054/251‑1 (MQ=255)                                                   gAACAGATAGCCCTGGCCGCGAACGGTGGTAATCACTTCTTGGGGATATTGTGCCTGAATTTTTTTGCGCAGACGTCCCATCAGTACATCAATGGTATGGCTTTCCCGCAGCTCTGCATCCGGATAGAGTTGGAGCATTAACGAATCTTTGCTGACCACTTTGCCATTATTGCGTATCAACGTTTCCATA‑ATGGTGTATTCGAACGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGCg                                                                                                                                                                                         >  1:391319/1‑249 (MQ=255)                                                      cAGATAGCCCTGGCCGCGAACGGTGGTAATCACTTCTTGGGGATATTGTGCCTGAATTTTTTTGCGCAGCCGTCCCATCAGTACATCAATGGTATGGCTTTCCCGCAGCTCCGCATCCGGATAGAGTTGGAGCATTAACGACTCTTTGCTGACCACTTTGCCATTATTGCGTATCAACGTTTCCATA‑ATGGTGTATTCGACCGCGGTCAGTTTGTTCACTTCGCCATTAATAGATAATTCACTGCgaa                                                                                                                                                                                       >  2:653575/1‑247 (MQ=255)                                                       aGATAGCCCTGGCCGCGAACGGTGGTAATCACTTCTTGGGGATATTGTGCCTGAATTTTTTTGCGCAGACGTCCCATCAGTACATCAATGGTATGGCTTTCCCGCAGCTCCGCATCCGGATAGAGTTGGAGCATTAACGAATCTTTGCTGACCACTTTGCCATTATTGCGTATCAACGTTTCCATA‑ATGGTGTAGTCGAACGCGGTCAGTTTGATCACGTCGTCATTAATAGATAATTCACGGCgagagag                                                                                                                                                                                   <  1:370780/251‑1 (MQ=255)                                                         aTAGCCCTGGCCGCGAACGGTGGTAATCACTTCTTGGGGATATTGTGCCTGAATTTTTTTGCGCAGACGTCCCATCAGTACATCAATGGTATGGCTTTCCCGCAGCTCCGCATCCGGATAGAGTTGGAGCATTAACGAATCTTTGCTGACCACTTTGCCATTATTGCGTATCAACGTTTCCATA‑ATGGTGTATTCGAACGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGCGAGAGAGAt                                                                                                                                                                                 <  1:738847/251‑1 (MQ=255)                                                                      cgAACGGTGGTAATCACTTCTTGGGGATATTGTGCCTGAATTTTTTTGCGCAGACGTCCCATCAGTACATCAATGGTATGGCTTTCCCGCAGCTCCGCATCCGGATAGAGTTGGAGCATTAACGAATCTTTGCTGACCACTTTGCCATTATTGCGTATCAACGTTTCCATA‑ATGGTGTATTCGACCGCGGTCAGTTTGATCACTTCGTCATTACTAGATAATTCACGGCGAGAGAGATCAACCTGAACCgg                                                                                                                                                                    >  2:501485/1‑251 (MQ=255)                                                                                  aTCACTTCTTGGGGATATTGTGCCTGAATTTTTTTGCGCAGACGTCCCAACAGTACATCAATGGTATGGCTTTCCCGCAGCTCCGCATCCGGATAGAGTTGGAGCATTAACGAATCTTTGCTGACCACTTTGCCATTATTGCGTATCAACGTTTCCATA‑ATGGTGTATTCGAACGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGCGAGAGAGATCAACCTGAAACGGGGGG‑AGCGAAAt                                                                                                                                                       <  2:66419/251‑1 (MQ=255)                                                                                    cACTTCTTGGGGATATTGTGCCTGAATTTTTTTGCGCAGACGTCCCATCAGTACATCAATGGTATGGCTTTCCCGCAGCTCCGCATCCGGATAGAGTTGGAGCATTAACGAATCTTTGCTGACCACTTTGCCATTATTGCGTATCAACGTTTCCATA‑ATGGTGTATTCGAACGCGGTCAGTTTGATAACTTCGTCATTAATAGATAATTCACGGCGAGAGAGATCAACCTGAAACGGGGGG‑AGCGAAATg                                                                                                                                                      >  2:107070/1‑250 (MQ=255)                                                                                     aCTTCTTGGGGATATTTTGCCTGAATTTTTTTGCGCAGACGTCCCATCAGTACATCAATGGTATGGCTTTCCCGCAGCTCCGCATCCGGATAGAGTTTGAGCATTAACGAATCTTTGCTGACCCCTTTGCCATTATTGCGTATCAACGTTTCCATA‑ATGGTGTATTCGAACGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGCGAGAGAGATCAACCTGAAACGGGGGG‑AGCGAAATGAc                                                                                                                                                    <  1:976083/251‑1 (MQ=255)                                                                                     aCTTCTTGGGGATATTGTGCCTGAATTTTTTTGCGCAGACGTCCCATCAGTACATCAATGGTATGGCTTTCCCGCAGCTCCGCATCCGGATAGAGTTGGAGCATTAACGAATCTTTGCTGACCACTTTGCCATTATTTCGTATCAACGTTTCCATA‑ATGGTGTATTCGAACGCGGTCAGTTTGATCACTTCGTCTTTAATAGTTAATCCCCGGCGAGAGAGTTCAACCTGAACCGGGGGGGAG‑GAAATGAc                                                                                                                                                    >  1:250786/1‑251 (MQ=255)                                                                                            ggggATATTGTGCCTGAATTTTTTTGCGCAGACGTCCCATCAGTACATCAATGGTATGGCTTTCCCGCAGCTCCGCATCCGGATAGAGTTGGAGCATTAACGAATCTTTGCTGACCACTTTGCCATTATTGCGTATCAACGTTTCCATA‑ATGGTGTATTCGAACGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGCGAGAGAGATCAACCTGAAACGGGGGG‑AGCGAAATGACCTGTGa                                                                                                                                              <  2:363548/250‑1 (MQ=255)                                                                                                  atTGTGCCTGAATTTTTTTGCGCAGACGTCCCATCAGTACATCAATGGTATGGCTTTCCCGCAGCTCCGCATCCGGATAGAGTTGGAGCATTAACGAATCTTTGCTGACCACTTTGCCATTATTGCGTATCAACGTTTCCATA‑ATGGTGTATTCGAACGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGCGAGAGAGATCAACCTGAAACGGGGGG‑AGCGAAATGACCTGTGAAGCCAGa                                                                                                                                       <  1:960700/251‑1 (MQ=255)                                                                                                        ccTGAATTTTTTTGCGCAGACGTCCCATCAGTACATCAATGGTATGGCTTTCCCGCAGCTCCGCATCCGGATAGAGTTGGAGCATTAACGAATCTTTGCTGACCACTTTGCCATTATTGCGTATCAACGTTTCCATA‑ATGGTGTATTCGAACGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGCGAGAGAGATCAACCTGAAACGGGGGG‑AGCGAAATGACCTGTGAAGCCAGACCGCTa                                                                                                                                 <  2:870050/251‑1 (MQ=255)                                                                                                                 ttttGCGCAGACGTCCCATCAGTACATCAATGGTATGGCTTTCCCGCAGCTCCGCATCCGGATAGAGTTGGAGCATTAACGAATCTTTGCTGACCACTTTGCCATTATTGCGTATCAACGTTTCCATA‑ATGGTGTATTCGAACGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGCGAGAGAGATCAACCTGAAACGGGGGG‑AGCGAAATGACCTGTGAAGCCAGACCGCTATTAcgccgc                                                                                                                        >  1:113733/1‑251 (MQ=255)                                                                                                                 ttttGCGCAGACGTCCCATCAGTACATCAATGGTATGGCTTTCCCGCAGCTCCGCATCCGGATAGAGTTGGAGCATTAACGAATCTTTGCTGACCACTTTGCCATTATTGCGTATCAACGTTTCCATA‑ATGGTGTATTCGAACGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGCGAGAGAGATCAACCTGAAACGGGGGG‑AACGAAATGACCTGGGAAGCCAGACCGCTATTAcgccgc                                                                                                                        >  2:823536/1‑251 (MQ=255)                                                                                                                   ttGCGCAGACGTCCCATCAGTACATCAATGGTATGGCTTTCCCGCAGCTCCGCATCCGGATAGAGTTGGAGCATTAACGAATCTTTGCTGACCACTTTGCCATTATTGCGTATCAACGTTTCCATA‑ATGGTGTATTCGAACGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGCGAGAGAGATCAACCTGAAACGGGGGG‑AGCGACATGACCTGAGAAGCCAGGCCGCTATTACGCCGCAt                                                                                                                      >  2:723400/1‑251 (MQ=255)                                                                                                                    tGCGCAGACGTCCCATCAGTACATCAATGGTATGGCTTTCCCGCAGCTCCGCATCCGGATAGAGTTGGAGCATTAACGAATCTTTGCTGACCACTTTGCCATTATTGCGTATCAACGTTTCCATA‑ATGGTGTATTCGAACGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGCGAGAGAGATCAACCTGAAACGGGGGG‑AGCGAAATGACCTGTGAAGCCAGACCGCTATTACGCCGCAtt                                                                                                                     >  1:79117/1‑251 (MQ=255)                                                                                                                       gcAGACGTCCCATCAGTACATCAATGGTATGGCTTTCACGCAGCTCCGCATCCGGATAGAGTTGGAGCATTAACGAATCTTTGCTGCCCACTTTGCCATTATTGCGTAACAACGTTTCCATA‑ATGGTGTATTCGAACGCGGTCAGTTTGATCAATTCGTCATTAATAGCTAATTCACGGCGAGAGAGATCAACCTGACACGGGGGG‑CGCGAAATGACCTGAGAAGCCAGACCGCTATTACGCCGCATTAAt                                                                                                                  >  2:280298/1‑251 (MQ=255)                                                                                                                         aGACGTCCCATCAGTACATCAATGGTATGGCTTTCCCGCAGCTCCGCATCCGGATAGAGTTGGAGCATTAACGAATCTTTGCTGACCACTTTGCCATTATTGCGTATCAACGTTTCCATA‑ATGGTGTATTCGAACGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGCGAGAGAGATCAACCTGAAACGGGGGG‑AGCGAAATGACCTGTGAAGCCAGACCGCTATTACGCCGCATTAATGc                                                                                                                >  1:744532/1‑251 (MQ=255)                                                                                                                                    cAGTACATCAATGGTATGGCTTTCCCGCAGCTCCGCATCCGGATAGAGTTGGAGCATTAACGAATCTTTGCTGACCACTTTGCCATTATTGCGTATCAACGTTTCCATA‑ATGGTGTATTCGAACGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGCGAGAGAGATCAACCTGCAACGGGGGG‑AGCGAAATGACCTGTGAAGCCAGACCGCTATTACGCCGCATTAATGCCTGCATTcgcg                                                                                                     >  1:152926/1‑251 (MQ=255)                                                                                                                                       aaCATCAATGGTATGGCTTTCCCGCAGCTCCGCATCCGGATAGAGTTGGAGCATTAACGAATCTTTGCTGACCACTTTGCCATTATTGCGTATCAACGTTTCCCTA‑ATGGTGTATTCGACCGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGCGAGAGAGATCAACCTGAAACGGGGGG‑AGCGAAATGACCTGTGAAGCCAGACCGCTATTACGCCGCATTACTGCCTGCATTcgcacc                                                                                                   >  2:197451/2‑247 (MQ=255)                                                                                                                                        aCATCAATGGTATGGCTTTCCCGCAGCTCCGCATCCGGATAGAGTTGGAGCATTAACGAATCTTTGCTGACCACTTTGCCATTATTGCGTATCAACGTTTCCATA‑ATGGTGTATTCGAACGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGCGAGAGAGATCAACCTGAAACGGGGGG‑AGCGAAATGACCTGTGAAGCCAGACCGCTATTACGCCGCATTAATGCCTGCATTCGCGCCAt                                                                                                 >  2:34698/1‑251 (MQ=255)                                                                                                                                           tCAATGGTATGGCTTTCCCGCAGCTCCGCATCCGGATAGAGTTGGAGCATTAACGAATCTTTGCTGACCACTTTGCCATTATTGCGTATCAACGTTTCCATA‑ATGGTGTATTCGAACGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGCGAGAGAGATCAACCTGAAACGGGGGG‑AGCGAAATGACCTGTGAAGCCAGACCGCTATTACGCCGCATTAATGCCTGCATTCGCGCCATCAc                                                                                              >  1:142400/1‑251 (MQ=255)                                                                                                                                            caatggtcagccttacccGCAGCTCCGCATACAGATAGAGTTCGAGCCATAACGAATCTTTGCTCACCACTTTGCCATTATTGCGTATCAACGTTTCCATA‑ATGGACTCTTAGAACGCGGTAAGTTTGATCACTTCGTCATTAATAGATAATTCACGGCGAGAGAGATCAACCTGAAACGGGGGG‑CGTGAAATGCCGTGTAAAGCCAGACCGATATTACGCCGCAGTACTGCCTGCATTCccgcaaccacc                                                                                             >  2:936072/16‑244 (MQ=255)                                                                                                                                                 gTATGGCTTTCCCGCAGCTCCGCATCCGGATAGAGTTGGAGCATTAACGAATCTTTGCTGACCACTTTGCCATTATTGCGTATCAACGTTTCCATA‑ATGGTGTAGTCGAACGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGCGAGAGAGATCAACCTGAAACGGGGGG‑AGCGAAATGACCTGTGAAGCCAGACCGCTATTACGCCGCATTAATGCCTGCATTCGCGCCATCACCGCTTc                                                                                        <  2:523232/251‑1 (MQ=255)                                                                                                                                                       cTTTCCCGCAGCTCCGCATCCGGATAGAGTTGGAGCATTAACGAATCTTTGCTGACCACTTTGCCATTATTGAGTATCAACGTTTCCATA‑ATGGTGTATTCGAACGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGCGAGAGAGATCAACCTGAAACGGGGGG‑AGCGAAATGACCTGTGAAGCCAGACCGCTATTACGCCGCATTAATGCCTGCATTCGCGCCATCACCTCTTCACTATg                                                                                  >  1:590994/1‑251 (MQ=255)                                                                                                                                                                aGCTCCGCATCCGGATAGAGTTGGAGCATTAACGAATCTTTGCTGACCACTTTGCCATTATTGCGTATCAACGTTTCCATA‑ATGGTGTATTCGAACGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGCGAGAGAGATCAACCTGAAACGGGGGG‑AGCGAAATGACCTGTGAAGCCAGACCGCTATTACGCCGCATTAATGCCTGCATTCGCGCCATCACCTCTTCAATATGAAACGGttt                                                                         <  1:849502/251‑1 (MQ=255)                                                                                                                                                                        aTCCGGATAGAGTTGGAGCATTAACGAATCTTTGCTGACCACTTTGCCATTATTGCGTATCAACGTTTACATA‑ATGGTGTATTCGAACGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGCGAGAGAGATCAACCTGAAACGGGGGG‑AGCGCAATGACCTGTGACGCCAGACAGCTATTACGCCGCATTAATGCCTGCATTCGCGCCATCACCTCTTCAATATGAAACGGTTTAGTCACAt                                                                 >  2:812320/1‑251 (MQ=255)                                                                                                                                                                           cGGAAAGAGTTGGAGCATTAACGAATCTTTGCTGACCACTTTGCCATTATTGCGTATCAACGTTTCCATA‑ATGGTGTATTCGAACGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGCGAGAGAGATCAACCTGAAACGGGGGG‑AGCGAAATGACCTGTGAAGCCAGACCGCTATTACGCCGCATTAATGCCTGCATTCGCGCCATCACCTCTTCAATATGAAACGGGTTTGTCCCATAat                                                              >  1:490905/1‑251 (MQ=255)                                                                                                                                                                                agtgTTGGTGCATTAACAAATCTTTGCTGACCACTTTGCCATTATTGCGGATCAACGTTTCCATA‑ATGGTGTAGTCGAACGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGCGAGAGAGATCAACCTGAAACGGGGGG‑AGCGAAATGACCTGTGAAGCCAGACCGCTATTACGCCGCATTAATGCCTGCATTCGCGCCATCGCCTCTTCAATATGAAACGGTTTAGTCACATAAtcatca                                                         <  2:287543/248‑1 (MQ=255)                                                                                                                                                                                  agTTGGAGCATTAACGAATCTTTGCTGACCACTTTGACATTATTGCGTATCAACGTTTCCATA‑ATGGTGTATTCGAACGCGATCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGCGAGAGAGATCAACCTGAAACGGGGGG‑AGCGAAATGACCTGTGACGCCAGACAGCTATTACGCCGCATTAATGCCTGCATTCGCGCCATCACCTCTTCAATATGAAACGGTTTAGTCAAATAatcctccgc                                                       >  2:484545/1‑245 (MQ=255)                                                                                                                                                                                     tggggCATTAACGAATCTTTGCTGACCACTTTGCCATTATTGCGTATCAACGTTTCCATA‑ATGGTGTATTCGAACGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGCGAGAGAGATCAACCTGAAACGGGGGG‑AGCGAAATGACCTGTGAAGCCAGACCGCTATTACGCCGCATTAATGCCTGCATTCGCGCCATCACCTCTTCAATATGAAACGGTTTAGTCACATAATCATCAGCAc                                                     <  1:883355/246‑1 (MQ=255)                                                                                                                                                                                      tgAGCATTAACGAATCTTTGCTGACCACTTTGCCATTATTGCGTATCAACGTTTCCATA‑ATGGTGTATTCGAACGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGCGAGAGAGATCAACCTGAAACGGGGGG‑AGCGAAATGACCTGTGAAGCCAGACCGCTATTACGCCGCATTAATGCCTGCATTCGCGCCATCACCTCTTCAATATGAAACGGTTTAGTCACATAATCATCAGCAcc                                                    <  1:85611/249‑1 (MQ=255)                                                                                                                                                                                              aaCGAATCTTTGCTGACCACTTTGCCATTATTGCGTATCAACGTTTCCATA‑ATGGTGTATTCGAACGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGCGAGAGAGATCAACCTGAAACGGGGGG‑AGCGAAATGACCTGTGAAGCCAGACCGCTATTACGCCGCATTAATGCCTGCATTCGCGCCATCACCTCTTCAATATGAAACGGTTTAGTCACATAATCATCAGCACCGGCACTTa                                            >  1:842245/1‑250 (MQ=255)                                                                                                                                                                                                 gAATCTTTGCTGACCACTTTGCCATTATTGCGTATCAACGTTTCCATA‑ATGGTGTATTCGAACGCGGTCAGTTTGAGCACTTCGTCATTAATAGATAATTCACGGCGAGAGAGATCAACCTGAAACGGGGGG‑AGCGAAATGACCTGTGAAGCCAGACCGCTATTACGCCGCATTAATGCCTGCATTCGCGCCATCACCTCTTCAATATGAAACGGTTTAGTCACATAATCATCAGCACCGGCACTTAATAc                                        <  1:630181/251‑1 (MQ=255)                                                                                                                                                                                                        tGCTTACCACTTTTCCATTATTGCTTATCAACGTTTCCTTA‑ATGGTGTATTTGAACGCGTTCAGTTTTATCATTTCGGCATTAATAGATAATTCACGGCGAGAGAGAGCAACCTGAAACGGGGGG‑AGCGAAATGACCTGTGAAGCCAGATCTCTATTACGCCGCATTAATGCCGGCATTCGCGCCATCACCTCTTCAATATGAAACGGTTTAGTAACATAATCATCTGCACCGGCACTTAATACTTCGAc                                  <  2:989503/250‑1 (MQ=255)                                                                                                                                                                                                          cTGACCACTTTGCCATTATTGCGTATCAACGTTTCCATA‑ATGGTGTATTCGAACGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGCGAGAGAGATCAACCTGAAACGGGGGG‑AGCGAAATGACCTGTGAAGCCAGACCGCTATTACGCCGCATTAATGCCTGCATTCGCGCCATCACCTCTTCAATATGAAACGGTTTAGTCACATAATCATCAGCACCGGCACTTAATACTTCGACttt                               >  1:868717/1‑251 (MQ=255)                                                                                                                                                                                                             aCCACTTTGCCATTATTGCGTATCAACGTTTCCATA‑ATGGTGTATTCGAACGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGCGAGAGAGATCAACCTGAAACGGGGGG‑AGCGAAATGACCTGTGAAGCCAGACCGCTATTACGCCGCATTAATGCCTGCATTCGCGCCATCACCTCTTCAATATGAAACGGTTTAGTCACATAATCATCAGCACCGGCACTTAATACTTCGACTTTGTc                            >  1:807856/1‑251 (MQ=255)                                                                                                                                                                                                             aCCACTTTGCCATTATTGCGGATCAACGTTTCCATA‑ATGGTGTATTCGAACGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGCGAGAGAGATCAACCTGAAACGGGGGG‑AGCGAAATGACCTGTGAAGCCAGACCGCTATTACGCCGCATTAATGCCTGCATTCGCGCCATCACCTCTTCAATATGAAACGGTTTAGTCACATAATCATCAGCACCGGCACTTAATACTTCGACTTTGTc                            <  2:79117/251‑1 (MQ=255)                                                                                                                                                                                                               cACTTTGCCATTATTGCGTATCAACGTTTCCATA‑ATGGTGTATTCGAACGAGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGCGAGAGAGATCAACCTGAAACGGGGGG‑AGCGAAATGACCTGTGAAGCCAGACCGCTATTACGCCGCATTAATGCCTGCATTTGCGCCATCACCTCTTCAATATGAAACGGTTTAGTCACATAATCATCAGCACCGGCCCTTAATACTTAGACTTTGTCCt                          >  2:945278/1‑251 (MQ=255)                                                                                                                                                                                                                aCTTTGCCATTATTGCGAATCAACGTTTCCATA‑ATGGTGTATTCGAACGCTGTCAGTTTGATCACTTCGTCCTTAATAGATAATTCACGGCGAGCGAGATCAACCTGAAACGGGTGG‑AGCAAAATGACCTGTGAAGCAAGACCGCTATTACTCAGCATTACTGCCTGCATTCGCGCCATCACCTCTTCAATATGAACCGGTTTATTCACATACTCATCTGCACCGGCACTTAATACTTCGACTTTGTCCTg                         >  2:255070/1‑251 (MQ=255)                                                                                                                                                                                                                  attGCCATTATAGCGTATCAACGTTTCCATA‑ATGGTGTATTCGAACGCGGTCAGTTAGATCACTTCGTCATTAATAGATAATTCACGGCGAGAGAGATCAACCTGAAACGGGGGG‑AGCGAAATGACCTGTGAAGCCAGACCGCTATTACGCCGCATTAATGCCTGCATTCGCGACATCACCTCTTAAATATGAAACGGTTTAGTCACATAATCATCAGCACCGGCACTTAATACTTAGACTTTGTCCTGcc                       >  2:355789/2‑251 (MQ=255)                                                                                                                                                                                                                     gCCATTATTGCGTATCAACGTTTCCATA‑ATGGTGTATTCGAACGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGCGGGAGAGATCAACCTGAAACGGGGGG‑AGCGAAATGACCTGTGAAGCCAGACCGCTATTACGCCGCATTAATGCCTGCATTCGCGCCATCACCTCTTCAATATGAACCGGTTTAGTCACATAATCATCAGCACCGGCACTTATTACTTCGACTTTGTCCTGCCAGc                    >  1:10357/1‑251 (MQ=255)                                                                                                                                                                                                                             tGCGTATCAACGTTTCCATA‑ATGGTGTATTCGAACGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGCGAGAGAGATCAACCTGAAACGGGGGG‑AGCGAAATGACCTGTGAAGCCAGACCGCTATTACGCCGCATTCATGCCTGCATTCGCGCCATCACCTCTTCAATATGAAACGGTTTAGTCACATAATCATCAGCACCGGCACTTAATACTTCGACTTTGTCCTGCCAGCTTTCACgg            >  1:640927/1‑251 (MQ=255)                                                                                                                                                                                                                                gTATAAACGTTTCCATA‑ATGGTGTATTCGAACGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGCGAGAGAGATCAACCTGAAACGGGGGG‑AGCGAAATGACCTGTGAAGCCAGACAGCTATTACGCCGCATTAATGCCTGCATTCGCGCCATCACCTCTTCAATATGAAACGGTTTAGTCACATAATCATCAGCACCGGCACTTAATACTTCGACTTTGTCCTGCCAGCTTTAACGGGCg         >  1:88306/1‑251 (MQ=255)                                                                                                                                                                                                                                       cGTTTCCATA‑ATGGTGTATTCGAACGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGCGAGAGAGATCAACCTGAAACGGGGGG‑AGCGAAATGACCTGTGAAGCCAGACCGCTATTACGCCGCATTAATGCCTGCATTCGCGCCATCACCTCTTCAATATGAAACGGTTTAGTCACATAATCATCAGCACCGGCACTTAATACTTCGACTTTGTCCTGCCAGCTTTCACGGGCGGTTAATa  <  1:146378/251‑1 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                CAGCGAGAGCGGGAAAAAAAGACGCAGTAATTTTTTCATCAGCGCAATTCGAACAGATAGCCCTGGCCGCGAACGGTGGTAATCACTTCTTGGGGATATTGTGCCTGAATTTTTTTGCGCAGACGTCCCATCAGTACATCAATGGTATGGCTTTCCCGCAGCTCCGCATCCGGATAGAGTTGGAGCATTAACGAATCTTTGCTGACCACTTTGCCATTATTGCGTATCAACGTTTCCATA‑ATAGTGTATTCGAACGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGCGAGAGAGATCAACCTGAAACGGGGGG‑AGCGAAATGACCTGTGAAGCCAGACCGCTATTACGCCGCATTAATGCCTGCATTCGCGCCATCACCTCTTCAATATGAAACGGTTTAGTCACATAATCATCAGCACCGGCACTTAATACTTCGACTTTGTCCTGCCAGCTTTCACGGGCGGTTAATA  >  NC_000913/1189738‑1190218

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑