Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	160,347	C→T	L86L (CTG→CTA)	dksA ←	RNA polymerase‑binding transcription factor DksA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	160,347	0	C	T	98.9%	283.7 / ‑4.6	91	L86L (CTG→CTA)	dksA	RNA polymerase‑binding transcription factor DksA
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base T (45/44);  total (46/45)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.49e-01

CGGGAGCATTTCCCGCCTGTGGTAAACGTGATGGAACGGCTGTAATTAGCCAGCCATCTGTTTTTCGCGAATTTCAGCCAGCGTTTTGCAGTCGATGCACAGATCGGCTGTCGGGCGCGCTTCCAGACGGCGAATACCAATTTCAACACCGCAGGATTCGCAGTAGCCGAAATCTTCGTCTTCCACTTTTTTCAGCGTCTTCTCGATCTTTTTGATCAGCTTACGCTCGCGATCGCGGTTACGCAGTTCGAGGCTGAACTCTTCTTCCTGGGCTG‑CACGGTCTACCGGGTCCGGGAAGTTGGCTGCTTCATCCTGCATATGTGTAACGGTGCGATCGACTTCATCCCTGAGTTGATTACGCCATGCTTCCAGAATACGACGGAAGTGCGCCAGCTGGGCTTCATTCATATACTCTTCGCCCGGCTTCTCCTGATATGGTTCCACCCCAGCGATGGCGAGAATACTCAGGGACGATGTTTTACGGTTTTG  >  NC_000913/160104‑160592                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                       cGGGAGCATTTCCCGCCTGTGGTAAACGTGATGGAACGGCTGTAATTAGCCAGCCATCTGTTTTTCGCGAATTTCAGCCAGCGTTTTGCAGTCGATGCACAGATCGGCTGTCGGGCGCGCTTCCAGACGGCGAATACCAATTTCAACACCGCAGGATTCGCAGTAGCCGAAATCTTCGTCTTCCACTTTTTTCAGCGTCTTCTCGATCTTTTTGATCAGCTTACGCTCGCGATCGCGGTTACGTAGTTCg                                                                                                                                                                                                                                                  >  1:234726/1‑250 (MQ=255)  gggAGCTTTTCCCGCCTGTGGTAAACGTGATGGAACGGCTGTAATTAGCCAGCCATCTGTTTTTCGCGAATTTCAGCCAGCGTTTTGCAGTCGATGCACAGATCGGCTGTCGGGCGCGCTTCCAGACGGCGAATACCAATTTCAACACCGCAGGATTCGCAGTAGCCGAAATCTTCGTCTTCCACTTTTTTCAGCGTCTTCTCGATCTTTTTGATCAGCTTACGCTCGCGATCGCGGTTACGTAGTTCGAg                                                                                                                                                                                                                                                <  1:893835/251‑1 (MQ=255)       cATTTCCCGCCTGTGGTAAACGTGATGGAACGGCTGTAATTAGCCAGCCATCTGTTTTTCGCGAATTTCAGCCAGCGTTTTGCAGTCGATGCACAGATAGGCTGTCGGGCGCGCTTCCAGACGGCGAATACCAATTTCAACACCGCAGGATTCGCAGTAGACGAAATCTTCGTCTTCCACTTTTTTCAGCGTCTTCTCGATCTTTTTGATCAGCTTAAGCTCGCGATCGCGGTTACGTAGTTCGAGGCTgg                                                                                                                                                                                                                                           >  1:31233/1‑250 (MQ=255)              cGCCTGTGGTAAACGTGATGGAACGGCTGTAATTAGCCAGCCATCTGTTTTTCGCGAATTTCAGCCAGCGTTTTGCAGTCGATGCACAGATCGGCTGTCGGGCGCGCTTCCAGACGGCGAATACCAATTTCAACACCGCAGGATTCGCAGTAGCCGAAATCTTCGTCTTCCACTTTTTTCAGCGTCTTCTCGATCTTTTTGATCAGCTTACGCTCGCGATCGCGGTTACGTAGTTCGAGGCTGAACtcttt                                                                                                                                                                                                                                    >  1:195641/1‑250 (MQ=255)              cGCCTGTGGTAAACGTGATGGAACGGCTGTAATTAGCCAGCCATCTGTTTTTCGCGAATTTCAGCCAGCGTTTTGCAGTCGATGCACAGATCGGCTGTCGGGCGCGCTTCCAGACGGCGAATACCAATTTCAACACCGCAGGATTCGCAGTAGCCGAAATCTTCGTCTTCCACTTTTTTCAGCGTCTTCTCGATCTTTTTGATCAGCTTACGCTCGCGATCGCGGTTACGTAGTTCGAGGCTGAACtcttt                                                                                                                                                                                                                                    >  2:446851/1‑250 (MQ=255)              cGCCTGTGGTAAACGTGATGGAACGGCTGTAATTAGCCAGCCATCTGTTTTTCGCGAATTACAGCCAGCGTTTTGCAGTCGATGCACAGATCGGCTGTCGGGCGCGCTTCCAGACGGCGAATACCAATTTCAACACCGCAGGATTCGCAGTAGCCGAAATCTTCGTATTCCACTTTTTTCAGCGTCTTCTCGATCTTTTTGATCAGCTTACGCTCGCGATCGCGGTTACGTAGTTCGAGGCTGAACtcttc                                                                                                                                                                                                                                    >  1:91071/1‑251 (MQ=255)              cGCCTGTGGTAAACGTGATGGAACGGCTGTAATTACCCAGCCATCTGGTTTTCGCGAATTTCAGCCAGCGTTTTGCAGTCGCTGCACAGATCGGCTGTCGGGCGCGCTTCCAGATGGCGACTACCAATTTCAACACCGCAGGATTCGTCGTAGCCGAAATCTTCGTCTTCCACTTTTTTTAGCGTCTTCTCGATCTTTTTGAACAGCTTTCGCTCGCGACCGCGGTTACGTCGTTCGCGGCTGGACtcttt                                                                                                                                                                                                                                    >  2:480079/1‑250 (MQ=255)                ccTGTGGTACACGTGATGGAACGGCTGTAATTAGCCAGCCCTCCGTTTTTCGCGACTTTCAGCCAGCGTTTTGCAGTCGATGCACAGATCGGCTGTCGGGCGAGCTTCCAGACGGCGAATACCCATTTCAAAACCGCAGGCTTCGCAGTAGCCGCAATCATCATCTTCAACTTTTTTCAGCCGCTTCTCGCAGTTTATGATCCGCTTCCGCTCGAGATCGGGGCTACGTAGTTCCAGGCTGACCCcttctt                                                                                                                                                                                                                                  >  2:898340/1‑251 (MQ=255)                   gtgGTAAACGTGATGGAACGGCTGTAATTAGCCAGCCATCTGTTTTTCGCGAATTTCAGCCAGCGTTTTGCAGTCGATGCACAGCTCGGCTGTCGGGCGCGCTTCCCGACGGCGAATACCCATTTCAACACCGCAGGATTCGCAGTAGCCGAAATATTCGTATTCCCCTTTTTTCAGCAGCTTCTCGCTCTTTTTGATCAGCTTACGCTCGCGATCGCGGTTACGTAGTTCGAGGCTGAACTCTTCTTCCt                                                                                                                                                                                                                               >  2:576660/1‑251 (MQ=255)                        aaaCGTGATGGAACGGCTGTAATTAGCCAGCCATCTGTTTTTCGCGAATTTCAGCCAGCGTTTGGCAGTCGACGCACAGAGCGGCTGACGGGCGCGCTTCCAGACGTCGAATACCAATTTCAACACCGCAGGATTCGCAGTAGCCGAAATCTTCGACTTCCACTTTTTTCAGCGTCTTCTTGATCTTTTTGATCAGCTTACGCTCGCGATCGCGGGTACGTAGTGCGAGGCTGAACTAGTCTTCCTGGGCt                                                                                                                                                                                                                          <  2:383453/251‑1 (MQ=255)                         aaCGTGATGGAACGGCTGTAATTAGCCAGCCATCTGTTTTTCGCGAATTTCAGCCAGCGTTTTGCAGTCGATGCACAGATCGGCTGTCGGGCGCGCTTCCAGACGGCGAATACCAATTTCAACACCGCCGGATTCGCAGTAGCCGAAATCTTCGTCTTCCACTTTTTTCAGCGTCTTCTCGATCTTTTTGATCAGCTTACGCTCGCGATCGCGGTTACGTAGTTCGAGGCTGAACTCTTTTTCCTGGGCTg                                                                                                                                                                                                                         >  2:301398/1‑251 (MQ=255)                         aaCGTGATGGAACGGCTGTAATTAGCCAGCCATCTGTTTTTCGCGAATTTCAGCCAGCGTTTTGCAGTCGATGCACAGATCGGCTGTCGGGCGCGCTTCCAGACGGCGAATACCAATTTCAACACCGCAGGATTCGCAGTAGCCGAAATCTTCGTCTTCCACTTTTTTCAGCGTCTTCTCGATCTTTTTGATCAGCTTACGCTCGCGTTCGCGGTTACGTAGTTCGAGGCTGAACTCTTCTTCCTGGGCTg                                                                                                                                                                                                                         >  2:470197/1‑251 (MQ=255)                           cGTGATGGAACGGCTGTAATTAGCCAGCCATCTGTTTTTCGCGAATTTCAGCCAGCGTTTTGCAGTCGATGCACAGATCGGCTGTCGGGCGCGCTTCCAGACGGCGAATACCAATTTCAACACCGCAGGATTCGCAGTAGCCGAAATCTTCGTCTTCCACTTTTTTCAGCGTCTTCTCGATCTTTTTGATCAGCTTACGCTCGCGATCGCGGTTACGTAGTTCGAGGCTGAACTCTTCTTCCTGGGCTG‑Ca                                                                                                                                                                                                                      <  1:963784/251‑1 (MQ=255)                                     cGGCTGTAATTAGCCAGCCATCTGTTTTTCGCGAATTTCAGCCAGCGTTTTGCAGTCGATGCACAGATCGGCTGTCGGGCGCGCTTCCAGACGGCGAATACCAATTTCAACACCGCAGGATTCGCAGTAGCCGAAATCTTCGTCTTCCACTTTTTTCAGCGTCTTCTCGATCTTTTTGATCAGCTTACGCTCGCGATCGCGGTTACGTAGTTCGAGGCTGAACTCTTCTTCCTGGGCTG‑CACGGTCTACCg                                                                                                                                                                                                            >  1:804618/1‑251 (MQ=255)                                        cTGTAATTAGCCAGCCATCTGTTTTTCGCGAATTTCAGCCAGCGTTTTGCAGTCGATGCACAGATCGGCTGTCGGGCGCGCTTCCAGACGGCGAATACCAATTTCAACACCGCAGGATTCGCAGTAGCCGAAATCTTCGTCTTCCACTTTTTTCAGCGTCTTCTCGATCTTTTTGATCAGCTTACGCTCGCGATCGCGGTTACGTAGTTCGAGGCTGAACTCTTCTTCCTGGGCTG‑CACGGTCTACCGGGt                                                                                                                                                                                                         >  1:802801/1‑251 (MQ=255)                                          gTAATTAGCCAGCCATCTGTTTTTCGCGAATTTCAGCCAGCGTTTTGCAGTCGATGCACAGATCGGCTGTCGGGCGCGCTTCCAGACGGCGAATACCAATTTCAACACCGCAGGATTCGCAGTAGCCGCACTCTTCGTCTTCCACTTTTTTCAGCGTCTTCTCGATCTTTTTGATCAGCTTACGCTCGCGATCGCGGTTACGTAGTTCGAGGCTGAACTCTTCTTCCTGGGCTG‑CACGGTCTCCCGGGTcc                                                                                                                                                                                                       >  2:328962/1‑251 (MQ=255)                                             aTTAGCCAGCCATCTGTTTTTCGCGAATTTCAGCCAGCGTTTTGCAGTCGATGCACAGATCGGCTGTCGGGCGCGCTTCCAGACGGCGAATACCAATTTCAACACCGCAGGATTCGCAGTAGCCGAAATCTTCGTCTTCCACTTTTTTCAGCGTCTTCTCGATCTTTTTGATCAGCTTACGCTCGCGATCGCGGTTACGTAGTTCGAGGCTGAACTCTTCTTCCTGGGCTG‑CACGGTCTACCGGGTCCggg                                                                                                                                                                                                    <  1:348876/251‑1 (MQ=255)                                               tAGCCAGCCAGCGGTTTTTCGCGAATTTCAGCCAGCGTTTTGCAGTCGATGCACAGATCGGCTGTCGGGCGCGCTTCCAGACGGCGAATACCAATTTCAACACCGCAGGATTCGCAGTAGCCGAAATCTTCGTCTTCCACTTTTTTCAGCGTCTTCTCGATCTTTTTGATCAGCTTACGCTCGCGATCGCGGTTACGTAGTTCGAGGCTGAACTCTTCTTCCTGGGCTG‑CACGGTCTACCGGGTCCGGGa                                                                                                                                                                                                   <  2:991753/250‑1 (MQ=255)                                                 gccagccaTCTGTTTTTCGCGAATTTCAGCAAGCGATTTGCAGTCGATGCACAGCTCGGCTGTCGGGCGCGCATACAGACGGCGAATACCAATTTCAACACCGCAGGATTCGCAGTAGCCAAAATCTTCGTCTTCAACTTTTTTCAGCGACTTCTCGATTTTTTTGATCAGCTTACACCCGCGATCGCGGATACGTAGTTCTGGGATGAACTTTTCTTCCAGGGCTGTCA‑GGTCTAcctgagccggga                                                                                                                                                                                                   >  2:692424/1‑238 (MQ=255)                                                   cagccaTCTGTTTTTCGCGAATTTCAGCCAGCGTTTTGCAGTCGATGCACAGATCGGCTGTCGGGCGCGCTTCCAGACGGCGAATACCAATTTCAACACCGCAGGATTCGCAGTAGCCGAAATCTTCGTCTTCCACTTTTTTCAGCGTCTTCTCGATCTTTTTGATCAGCTTACGCTCGCGATCGCGGTTACGTAGTTCGAGGCTGAACTCTTCTTCCTGGGCTG‑CACGGTCTACCGGGTCCGGGAAGTTg                                                                                                                                                                                              <  1:95754/251‑1 (MQ=255)                                                   cagccaTCTGTTGTTCTCGAATTTTAGCCAGCGGTTTGCAGTCGATGCACAGATCGGCTGTCGGGCGCGCTTCCAGACGGCGAATACCAATTTCAACACCGCAGGATTCGCAGTAGTCGAAATCTTCGGCTTCCACTTTTTTCAGCGTCTTCTCGATCTTTTTGATCAGCTTACGCTCGCGATCGCGGTTAAGTAGTTCGAGGCTGAACTCTTCTTCCTGGGCTG‑CACGGTCTACCGGGTCCGGGAAGTTg                                                                                                                                                                                              <  2:673561/251‑1 (MQ=255)                                                      ccaTCTGTTTTTCGCGAATTTCAGCCAGCGTTTTGCAGTCGATGCACAGATCGGCTGTCGGGCGCGCTTCCAGACGGCGAATACCAATTTCAACACCGCAGGATTCGCAGTAGCCGAAATCTTCGTCTTCCACTTTTTTCAGCGTCTTCTCGATATTTTTGATCAGCTTACGCTCGCGATAGAGGGTACGAAGTTTGAGGTTGAACGCTTCTTCCTGGGATG‑CACGGTCTACCggggacggggagtaggct                                                                                                                                                                                           >  2:534560/1‑236 (MQ=255)                                                      ccaTCTGCTTTTCGCGAATTTCAGCCAGCGTTTTGCAGTCGATGCACAGATCGGCTGCCGGGCGCGCTTCCAGACGGCGAATACCAATTTCAACACCGCAGGTTTCGCAGTAGCCGAAATCTTCGTCTTCCCCTTTTTTCAGCGTCTTCTCGATCTTTTTGATCAGCTTACGCCCGCGATCGCGGTTACGTAGTTCGAGGCTGAACTCTTCTTCCTGGGCCG‑CACGGTCTACCGGGTCCGGGAAGGTGgct                                                                                                                                                                                           >  1:710751/1‑251 (MQ=255)                                                       caTCCGTTTTTCGCGAATGTCAGCCAGCGTTTTGCAGTCGATGCACAGATCGGATGTCGGGCGCGTGTCCAGACGGCGAATACCAATTTCAACTCCGCAGGATTCGCAGTAGCCGAAATCTTCGTCTTCCACTTTTTTCAGCGTCTTCTCGATCTTTTTGATCAGCTTACGCGCGCGATCGCGGTTACGTAGTTCGAGGCTGATCTCTTCTTCCTGGGCTG‑CACGGTCTACCGGGTCCGGGAAGTTGgct                                                                                                                                                                                           <  2:215551/250‑1 (MQ=255)                                                                       aTTTCAGCCAGCGTTTTGCAGTCGATGCACAGATCGGCTGTCGGGCGCGCTTCCAGACGGCGAATACCAATTTCAACACCGCAGGATTCGCAGTAGCCGAAATCTTCGTCTTCCACTTTTTTCAGCGTCTTCTCGATCTTTTTGATCAGCTTACGCTCGCGATCGCGGTTACGTAGTTCGAGGCTGAACTCTTCTTCCTGGGCTG‑CACGGTCTACCGGGTCCGGGAAGTTGGCTGCTTCATCCTGCATAtg                                                                                                                                                                          <  1:661951/251‑1 (MQ=255)                                                                       aTTTCAGCCAGCGTTTTGCAGTCGATGCAAAGATCGGCTGTCGGGCGCGCTTCCAGACGGCGAATACCAATTTCAACACCGCAGGATTCGCAGTAGCCGAAATCTTCGTCTTCCACTTTTTTCAGCGTCTTCTCGATCTTTTTGATCAGCTTACGCTCGCGATCGCGGTTACGTAGTTCGAGGCTGAACTCTTCTTCCTGGGCTG‑CACGGTCTACCGGGTCCGGGAAGTTGGCTGCTTCATCCTGCATAtg                                                                                                                                                                          >  2:632483/1‑251 (MQ=255)                                                                          tCAGCCAGCGTTTTGCAGTCGATGCACAGATCGGCTGACGGGCGCGCTTCCAGACGGCGAATACCAATTTCAACAACGCAGGATTCGCAGTAGCCGAAATCTTCGTCTTCCACTTTTTTCAGCGTCTTCTCGATCTTTTTGATCAGCTTACGCTCGCGATCGCGGTTACGTAGTTCGAGGCTGAACTCTTCTTCCTGGGCTG‑CACGGTCTACCGGGTCCGGGAAGTTGGCTGCTTCATCCTGCATATgtgt                                                                                                                                                                       >  1:583309/1‑251 (MQ=255)                                                                           cagccagcGTTTTGCAGTCGATGCACAGATCGGCTGTCGGGCGCGCTTCCAGACGGCGAATACCAATTTCAACACCGCAGGATTCGCAGTAGCCGAAATCTTCGTCTTCCACTTTTTTCAGCGTCTTCTCGATCTTTTTGATCAGCTTACGCTCGCGATCGCGGTTACGTAGTTCGAGGCTGAACTCTTCTTCCTGGGCTG‑CACGGTCTACCGGGTCCGGGAAGTTGGCTGCTTCATCCTGCATATgtgt                                                                                                                                                                       <  1:284900/250‑1 (MQ=255)                                                                           cagccagcGTTTTGCAGTCGATGCAAAGATCGGCTGTCGGGCGCGCTTCCAGACGGCGAATACCAATTTCAACACCGCAGAATTCGCAGTAGCCGAAATCTTAGTCTTCCACTTTTTTCAGCGTCTTCTCGATCTTTTTGATCAGCTTACGCTCGCGATCGCGGTTACGCAGTTCGAGGCTGAACTCTTCTTCCTGGGCTG‑CACGGTCTACCGGGTCCGGGAAGTTGGCTGCTTCATCCTGCATATgtgt                                                                                                                                                                       <  1:925020/250‑1 (MQ=255)                                                                            agccagcGTTTTGCAGTCGATGCACAGATCGGCTGTCGGGCGCGCTTCCAGACGGCGAATACCAATTTCAACACCGCAGGATTCGCAGTAGCCGAAATCTTCGTCTTCCACTTTTTTCAGCGTCTTCTCGATCTTTTTGATCAGCTTACGCTCGCGATCGCGGTTACGTAGTTCGAGGCTGAACTCTTCTTCCTGGGCTG‑CACGGTCTACCGGGTCCGGGAAGTTGGCTGCTTCATCCTGCATATGTGTa                                                                                                                                                                      >  1:337101/1‑250 (MQ=255)                                                                              ccagcGTTTTGCAGTCGATGCACAGATCGGCTGTCGGGCGCGCTTCCAGACGGCGAATACCAATTTCAACACCGCAGGATTCGCAGTAGCCGAAATCTTCGTCTTCCACTTTTTTCAGCGTCTTCTCGATCTTTTTGATCAGCTTACGCTCGCGATCGCGGTTACGTAGTTCGTGGCTGAACTCTTCTTCCTGGGCTG‑CACGGTCTACCGGGTCCGGGAAGTTGGCTGCTTCATACTGCATATATGTAACg                                                                                                                                                                   >  2:976265/1‑251 (MQ=255)                                                                              ccagcGTTTTGCAGTCGATGCACAGATCGGCTGTCGGGCGCGCTTCCAGACGGCGAATACCAATTTCAACACCGCAGGATTCGCAGTAGCCGAAATCTTCGTCTTCCACTTTTTTCAGCGTCTTCTCGATCTTTTTGATCAGCTTACGCTCGCGATCGCGGTTACGTAGTTCGAGGCTGAACTCTTCTTCCTGGGCTG‑CACGGTCTACCGGGTCCGGGAAGTTGGCTGCTTCATCCTGCATATGTGTAACg                                                                                                                                                                   <  1:227084/251‑1 (MQ=255)                                                                                agcGTTTTGCAGTCGATGCACAGATCGGCTGTCGGGCGCGCTTCCCGACGGCGAATACCAATTTCAACACCGCAGGATTCGCAGTAGCCGAAATCTTCGTCTTCCACTTTTTTCAGCGTCTTCTCGATCTTTTTGATCAGCTTACGCTCGCGATCGCGGTTACGTAGTTCGAGGCTGAACTCTTCTTCCTGGGCTG‑CACGGTCTACCGGGTCCGGGAAGTTGGCTGCTTCATCCTGCATATGTGTAACggg                                                                                                                                                                 >  1:753421/1‑250 (MQ=255)                                                                                agcGTTTTGCAGTAGAAGAACAGATTGGATGTCGGGAGCGCTTCCAGACGGCGAATACCAATTTCAACACCGCAGGATTCGCAGTAGCCGAAATCTTCGTCTTCCACTTTTTTCAGCGTCTTCTCGATCTTTTTGATCAGCTTACGCTCGCTATCGCGGTTACGTAGTTCGAGGCTGAACTCTTCTTCCTGGGCTG‑CACGGTCTACCGGGTCCGGGAAGTTGGCTGCTTCATCCTGCATATGTGTAACGGt                                                                                                                                                                 >  1:701782/1‑251 (MQ=255)                                                                                     tttGCAGTCGATGCACAGATCGGCTGTCGGGCGCGCTTCCAGACGGCGAATACCAATTTCAACACCGCAGGATTCGCAGTAGCCGAAATCTTCGTCTTCCACTTTTTTCACCGTCTTCTCGATCTTTTTGCTCAGCTTACGCTCTCGATCGCGGTTACGTAGTTCGAGTCTGAACTCTTCTTCCTGGGCTG‑CACGGTCTACCGGGTCCGGGTAGTTGGCTGCTTCATCCTGCATATGTGTAACGGTGCGAt                                                                                                                                                            >  1:349204/1‑251 (MQ=255)                                                                                               aTGCACAGATCGGCTGTCGGGCGCGCTTCCAGACGGCGAATACCAATTTCAACACCGCAGGATTCGCAGTAGCCGAAATCTTCGTCTTCCACTTTTTTCAGCGTCTTCTCGATCTTTTTGATCAGCTTACGCTCGCGATCGCGGTTACGTAGTTCGAGGCTGAACTCTTCTTCCTGGGCTG‑CACGGTCTACCGGGTCCGGGAAGTTGGCTGCTTCATCCTGCATATGTGTAACGGTGCGATCGACTTCATc                                                                                                                                                  >  1:620726/1‑251 (MQ=255)                                                                                                  cccaGATCGGCGGGCGGGCGCGCTGCCGGACGGCGAATACCAATTCCAACACCGGAGGATTCTCAGTAGCCGAAATCTTCGTCTTCCACTTTTTGCAGCGTCTGCGCGATCTTTTTGATCAGCTTACGCTCGCGATCGCGGTGACGTAGTTCGAGGCTGATCTCTTCTTCCTGGGCTG‑CACGGTCTACCGGGTCCGGGAAGTTGGCTGTGTCATCCTGCATATGTGTAACGGTGCTATCGACTTCATCCCt                                                                                                                                               <  2:386880/249‑1 (MQ=255)                                                                                                   acaGATCGGCTGTCGGGCGCGCTTCCAGACGGCGAATACAAATTTCAACACCGCAGGATTCGCAGTAGCCGAAATCTTCGTCTTCCACTTTTTTCAGCGTCTTCTCGATCTTTTTGATCAGCTTACGCTCGCGATCGCGGTTACGTAGTTCGAGGCTGAACTCTTCTTCCTGGGCTG‑CACGGTCTACCGGGTCCGGGAAGTTGGCTGCTTCATCCTGCATATGTGTAACGGTGCGATCGACTTCATCCCTg                                                                                                                                              <  1:681281/251‑1 (MQ=255)                                                                                                     aGATCGGCTGTCGGGCGCGCTTCCAGACGGCGAATACCAATTTCAACACCGCAGGATTCGCAGTAGCCGAAATCTTAGTCTTCCACTTTTTTCAGCGTCTTCTCGATCTTTTTGATCAGCTTACGCTCGCGATCGCGGTTACGTAGTTCGAGGCTGAACTCTTCTTCCTGGGCTG‑CACGGTCTACCGGGTCCGGGAAGTTGGCTGCTTCATCCTGCATATGTGTAACGGGGCGATCGACTTCATCCCTg                                                                                                                                              >  2:745674/1‑249 (MQ=255)                                                                                                      gATCGGCTGTCGGGCGCGCTTCCAGACGGCGAATACCAATTTCAACACCGCAGGATTCGCAGTAGCCGAAATCTTCGTCTTCCACTTTTTTCAGCGTCTTCTCGATCTTTTTGATCAGCTTACGCTCGCGATCGCGGTTACGTAGTTCGAGGCTGAACTCTTCTTCCTGGGCTG‑CACGGTCTACCGGGTCCGGGAAGTTGGCTGCTTCATCCTGCATATGTGTAACGGTGCGATCGACTTCATCCCTGAGt                                                                                                                                           >  1:724318/1‑251 (MQ=255)                                                                                                       aTCGGCTGTCGGGCGCGCTTCCAGACGGCGAATACCAATTTCAACACCGCAGGATTCGCAGTAGCCGAAATCTTCGTCTTCCACTTTTTTCAGCGTCTTCTCGATCTTTTTGATCAGCTTACGCTCGCGATCGCGGTTACGTAGTTCGAGGCTGAACTCTTCTTCCTGGGCTG‑CACGGTCTACCGGGTCCGGGAAGTTGGCTGCTTCATCCTGCATATGTGTAACGGTGCGATCGACTTCATCCCTGAGtt                                                                                                                                          <  1:62820/251‑1 (MQ=255)                                                                                                         cGGCTGTCGGGCGCGCTTCCAGACGGCGAATACCACTTTCAACACCGCAGGATTCGCAGTAGCCGAAATCTTCGTCTTCCACTTTTTTCAGCGTCTTCTCGATCTTTTTGATCAGCTTACGCTCGCGATCGCGGTTACGTAGTTCGAGGCTGAACTCTTCTTCCTGGGCTG‑CACGGTCTACCGGGTCCGGGAAGTTGGCTGCTTCATCCTGCATATGTGTAACGGTGCGATCGACTTCATCCCTGAGTTg                                                                                                                                         >  1:254918/1‑250 (MQ=255)                                                                                                         cGGCTGTCGGGCGCGCTTCCAGACGGCGAATACCAATTTCAACACCGCAGGATTCGCAGTAGCCGAAATCTTCGTCTTCCACTTTTTTCAGCGTCTTCTCGATCTTTTTGATCAGCTTACGCTCGCGATCGCGGTTACGTAGTTCGAGGCTGAACTCTTCTTCCTGGGCTG‑CACGGTCTACCGGGTCCGGGAAGTTGGCTGCTTCATCCTGCATATGTGTAACGGTGCGATCGACTTCATCCCTGAGTTg                                                                                                                                         >  1:275790/1‑250 (MQ=255)                                                                                                          ggCTGTCGGGCGCGCTTCCAGACGGCGAATACCAATTTCAACACCGCAGGATTCGCAGTAGCCGAAATCTTCGCCTTCCACTTTTTTCAGCGTCTTCTCGATCTTTTTGATCAGCTTACGCTCGCGCTCGCGGTTACGTAGTTCGAGGCTGAACTCTTCTTCCTGGGCTG‑CACGGTCTACCGGGTCCGGGAAGTTGGCTGCTTCATCCTGCATATGTGTAACGGTGCGATCGACTTCTTCCCTGAGTTGAt                                                                                                                                       >  2:926912/1‑251 (MQ=255)                                                                                                           gCTGTCGGGCGCGCTTCCAGACGGCGAATACCAATTTCAACACCGCAGGATTCGCAGTAGCCGAAATCTTCGTCTTCCACTTTTTTCAGCGTCTTCTCGATCTTTTTGATCAGCTTACGCTCGCGATCGCGGTTACGTAGTTCGAGGCTGAACTCTTCTTCCTGGGCTG‑CACGGTCTACCGGGTCCGGGAAGTTGGCTGCTTCATCCTGCATATGTGTAACGGTGCGATCGCCTTCATCCCTGAGTTGAtt                                                                                                                                      >  2:974733/1‑251 (MQ=255)                                                                                                           gCTGGCGGGCGCGTCTCCAGACGGCGAATACCAATTTCTACTCCGCAGGAGTATGAGTAGCCGAAATCTTCGTCTTCCACTTTTTACTGCGTCTTCTTGATCTCTTTGTTCAGCTTCCACTCGCGCTCGCGGTTACGTAGTTCGTGGAGGAACTCTTCTTCCTGGGCTG‑CACGGTCTACCGGGGCCGGGAAGTTGGCTGCTGCATCTGGCATATGTGTAACGGTGCGATCGACTTCATCCGTGAGTTGAtt                                                                                                                                      <  2:608556/251‑1 (MQ=255)                                                                                                                cGGGCGCGCTTCCAGACGGCGAATACCAATTTCAACTCCGCAGGATTCGCAGTAGCCGAAATCTTCTTCTTCCACTTTTTTCAGCGTCTTCTCGATCTTTTTGATCATCTTACGCTCGCGATCGCGGTTACGTAGTTCGAGGCTGAACTCTTCTTCCTGGGCTG‑CACGGTCTACCGGGTCCGGGAAGTTGGCTGCTTCATCCTGCATATGTGTAACGGTGCGATCGACTTCATCCCTGAGTTGATTACGcc                                                                                                                                 <  1:898347/251‑1 (MQ=255)                                                                                                                cGGGCGCGCTTCCAGACGGCGAATACCAATTTCAACACCGCAGGATTCGCAGTAGCCGAAATCTTCGTCTTCCACTTTTTTCAGCGTCTTCTCGATCTTTTTGATCAGCTTACGCTCGCGATCGCGGTTACGTAGTTCGAGGCTGAACTCTTCTTCCTGGGCTG‑CACGGTCTACCGGGTCCGGGAAGTTGGCTGCTTCATCCTGCATATGTGTAACGGTGCGATCGACTTCATCCCTGAGTTGATGACGcc                                                                                                                                 <  1:199426/251‑1 (MQ=255)                                                                                                                              gACGGCGAATACCAATTTCAACCCCGCAGGATTCGCAGTAGCCGAAATCTTCGTCTTCCACTTTTTTCAGCGTCTTCTCGATCTTTTTGATCAGCTTACGCTCGCGATCGCGGTTACGTAGTTCGAGGCTGAACTCTTCTTCCTGGGCTG‑CACGGTCTACCGGGTCCGGGAAGTTGGCTGCTTCATCCTGCATATGTGTAACGGTGCGATCGACTTCATCCCTGAGTTGATTACGCCATGCTTCCAGAATa                                                                                                                   <  2:275790/251‑1 (MQ=255)                                                                                                                               actgCGAATCCCAATTTCAACACCTCAGGATTCGCAGTAGCCGAAATCTTCGTCTTCCGCTTTTTTCTGCGTCTTCTCGTTCTTTTGGAGCAGCTTACGCTCGCGATCGCGGTTACGTAGTTCGAGGGTGAACTCTTCTTCCTGGGCTG‑CACGGTCTACCGGGTCCGGGAAGTTGGCTGCTTCATCCTGCATATGTGTAACGGTGCGATCGACTTCATCCCTGAGTTGATTACGCCATGCGTCCAGAATac                                                                                                                  <  2:614136/248‑1 (MQ=255)                                                                                                                                        aCCAATTTCAACACCGCAGGATTCGCAGTAGCCGAAATCTTCGTCTTCCTCTTTTTTCAGCGTCTTCTCGATCTTTTTGATCAGCTTACGCTCGCGATCGCGGTTACGTAGTTCGAGGCTGAACTCTTCTTCCTGGGCTG‑CACGGTCTACCGGGTCCGGGAAGTTGGCTGCTTCATCCTGCATATGTGTAACGGTGCGATCGACTTCATCCCTGAGTTGATTACGCCATGCTTCCAGAATACGACGGAAGt                                                                                                         <  2:892286/251‑1 (MQ=255)                                                                                                                                           aaTTTCAACACCGCAGGATTCGCAGTAGCCGAAATCTTCGTCTTCCACTTTTTTCAGCGTCTTCTCGATCTTTTTGATCAGCTTACGCTCGCGATCGCGGTTACGTAGTTCGAGGCTGAACTCTTCTTCCTGGGCTG‑CACGGTCTACCGGGTcc                                                                                                                                                                                                       >  2:103332/1‑154 (MQ=255)                                                                                                                                           aaTTTCAACACCGCAGGATTCGCAGTAGCCGAAATCTTCGTCTTCCACTTTTTTCAGCGTCTTCTCGATCTTTTTGATCAGCTTACGCTCGCGATCGCGGTTACGTAGTTCGAGGCTGAACTCTTCTTCCTGGGCTG‑CACGGTCTACCGGGTcc                                                                                                                                                                                                       <  1:103332/154‑1 (MQ=255)                                                                                                                                           aaTTTCAACACCGCAGGATTCGCAGTAGCCGAAATCTTCGTCTTCCACTTTTTTCAGCGTCTTCTCGATCTTTTTGATCAGCTTACGCTCGCGATCGCGGTTACGTAGTTCGAGGCTGAACTCTTCTTCCTGGGCTG‑CACGGTCTACCGGGTCCGGGAAGTTGGCTGCTTCATCCTGCATATGTGTAACGGTGCGATCGACTTCATCCCTGAGTTGATTCCGCCATGCTTCCAGAATACGACGGAAGTgcg                                                                                                      >  2:192504/1‑251 (MQ=255)                                                                                                                                                 aaCACCGCAGGATTCGCAGTAGCCGAAATCTTCGTCTTCCACTTTTTTCAGCTTCTTCTCGATCTTTTTGATCAGCTTACGCTCGCGATCGCGGTTACGTAGTTCGAGGCTGAACTCTGCTTCCTGGGCTG‑CACGGTCTACCGGGGCCTGGAAGTTGGCTGCTTCATCCGGCATATGTGTAACGGTGCGATCGACTGCATCCCTGAGTTGATTACGCCATGCTACCAGAATACGACGGAAGTGCGCCAGCt                                                                                                <  1:957302/251‑1 (MQ=255)                                                                                                                                                   cacCGCAGGATTCGCAGTAGCCGAAATCTTCGTCTTCCACTTTTTTCAGCGTCTTCTCGATCTTTTTGATCAGCTTACGCTCGCGATCGCGGTTACGTAGTTCGAGGCTGAACTCTTCTTCCTGGGCTG‑CACGGTCTACCGGGTCCGGGAAGTTGGCTGCTTCATCCTGCATATGTGTAACGGTGCGATCGACTTCATCCCTGAGTTGATTACGCCATGCTTCCAGAATACGACGGAAGTGCGCCAGCTgg                                                                                              >  1:386952/1‑251 (MQ=255)                                                                                                                                                   cacCGCAGGATTCGCAGTAGCCGAAATCTTCGTCTTCCACTTTTTTCAGCGTCTTCTCGATCTTTTTGATCAGCTTACGCTCGCGATCGCGGTTACGTAGTTCGAGGCTGAACTCTTCTTCCTGGGCTG‑CACGGTCTACCGGGTCCGGGAAGTTGGCTGCTTCATCCTGCATATGTGTAACGGTGCGATCGACTTCATCCCTGAGTTGATTACGCCATGCTTCCAGAATACGACGGAAGTGCGCCAGCTgg                                                                                              >  1:971170/1‑251 (MQ=255)                                                                                                                                                   acccGCAGGATGCGCAGTAGCCGAAATCTTCGTCTTCCACTTTTTTCAGCGTCTTCTCGATCTTTTTGATCAGCTTACGCTCGCGATCGCGGTTACGTAGTTCGAGGCTGAACTCTTCTTCCTGGGCTG‑CACGGTCTACCGGGTCCGGGAAGTTGGCTGCTTCATCCTGCATATGTGTAACGGTGCGATCGACTTCATCCCTGAGTTGATTACGCCATGCTTCCAGAATACGACGGAAGTGCGCCAGCTgg                                                                                              <  2:330442/249‑1 (MQ=255)                                                                                                                                                      cGCAGGATTCGCAGTAGCCGAAATCTTCGTCTTCCACTTTTTTCAGCGTCTTCTCGATCTTTTTGATCAGCTTACGCTCGCGATCGCGGTTACGTAGTTCGAGGCTGAACTCTTCTTCCTGGGCTG‑CACGGTCTACCGGGTCCGGGAAGTTGGCTGCTTCATCCTGCATATGTGTAACGGTGCGATCGACTTCATCCCTGAGTTGATTACGCCATGCTTCCAGAATACGACGGAAGTGCGCCAGCTGGGCt                                                                                           <  1:124118/251‑1 (MQ=255)                                                                                                                                                        cAGGATTCGCAGTAGCCGAAATCTTCGTCTTCCACTTTTGTCAGCGTCTTCTCGATCTTTTTGATCAGCTTACGCGCGCGATCGCGGTTACGTAGTTCGAGGCTGAACTCTTCTTCCTGGGCTG‑CACGGTCTACCGGGTCCGGGAAGTTGGCTGCTTCATCCTGCATATGTGTAACGGTGCGATCGACTTCATCCCTGAGTTGATTACGCCATGCTTCCAGAATACGACGGAAGTGCGCCAGCTGGGCttc                                                                                         <  2:257868/251‑1 (MQ=255)                                                                                                                                                         aGGATTCGCAGTAGCCGAAATCTTCGTCTTCCACTTTTTTCAGCGTCTTCTCGATCTTTTTGATCAGCTTACGCTCGCGATCGCGGTTACGTAGTTCGAGGCTGAACTCTTCTTCCTGGGCTG‑CACGGTCTACCGGGTCCGGGAAGTTGGCTGCTTCATCCTGCATATGTGTAACGGTGCGATCGACTTCATCCCTGAGTTGATTACGCCATGCTTCCAGAATACGACGGAAGTGCGCCAGCTGGGCttca                                                                                        <  1:315321/251‑1 (MQ=255)                                                                                                                                                            attggcagtaagctaaacccgcGTCTTCCACTTTTTTCATCTGCTTCGCGATCTTTTTGATCAGCTTACGCTCGCGATCGCGGTTACGTAGTTCGAGGCTTAACTCTTCTTCCTGGGCTG‑CACGGTCTACCGGGTCCGGGAAGTTGGCGGCTTCATCCTGCATATGTGTAACGGTGCGATCGACTGCATCCCTGAGTTGATTACGCCATGCTTCCAGAATACGAAGGAAGTGCGCGAGCTGTGCttcattc                                                                                     <  2:915234/230‑1 (MQ=255)                                                                                                                                                               cGCAGTAGTCTAAATCTTCGTCTTCCTCTTTTTTCAGCGTCTTCTCGATCTTTTTGATCAGCTTACGCTCGCGATCGCGGTTACGTAGTTCGAGGCTGAACTCTTCTTCCTGGGCTG‑CACGGTCTACCGGGTCCGGGAAGTTGGCTGCTTCATCCTGCATATGTGTAACGGTGCGATCGACTTCATCCCTGAGTTGATTACGCCATGCTTCCAGAATACGACGGAAGTGCGCCAGCTGGGCTTCATTCata                                                                                  <  2:968744/251‑1 (MQ=255)                                                                                                                                                               cGCAGTAGCCGAAATCTTCGTCTTCCACTTTTTTCAGCGTCTTCTCGATCTTTTTGATCAGCTTACGCTCGCGATCGCGGTTACGTAGTTCGAGGCTGAACTCTTCTTCCTGGGCTG‑CACGGTCTACCGGGTCCGGGAAGTTGGCTGCTTCATCCTGCATATGTGTAACGGTGCGATCGACTTCATCCCTGAGTTGATTACGCCATGCTTCCAGAATACGACGGAAGTGCGCCAGCTGGGCTTCATTCata                                                                                  <  1:745674/251‑1 (MQ=255)                                                                                                                                                               cGCAGTAGCCGAAAGCTTCGTCTTCCACTTTTTTCAGCGTCTTCTCGATCTTTTTGATCAGCTTACGCTCGCGATCGCGGTTACGTAGTTCGAGGCTGAACTCTTCTTCCTGGGCTG‑CACGGTCTACCGGGTCCGGGAAGTTGGCTGCTTCATCCTGCATATGTGTAACGGTGCGATCGACTTCATCCCTGAGTTGATTACGCCATGCTTCCAGAATACGACGGAAGTGCGCCAGCTGGGCTtcattcat                                                                                   <  1:480079/250‑1 (MQ=255)                                                                                                                                                                  ggTAGCCGAAACCTTCGTCTTCCCCTTTTTTCAGCGTCTTCTCGATCTTTTTGATCAGCTTACGCTCGCGATCGCGGTTACGTAGTTCGAGGCTGAACTCTTCTTCCTGGGCTG‑CACGGTCTACCGGGTCCGGGAAGTTGGCTGCTTCATCCTGCATATGTGTAACGGTGCGATCGACTTCATCCCTGAGTTGATTACGCCATGCTTCCAGAATACGACGGAAGTGCGCCAGCTGGGCTTCATTCATATAc                                                                               <  2:496069/250‑1 (MQ=255)                                                                                                                                                                  aGTAGCCGAAATCTTCGTCTTCCACTTTTTTCAGCGTCTTCTCGCTCTTTTTGATCAGATTACGCTCGCGATCGCGGTTACGTAGTTCGCGGCTGAACTCTTCTTCCTGGGCTG‑CACGGTCTACCGGGTCCGGGAAGTTGACTGCTTCATCCTGCATATGTGTAACGGTGCGATCGACTTCATCCCTGAGTTGCTTACGCCATGCTTCCAGAATACGACGGAAGTGCGCCAGCTGGGCTTCATTCATATAc                                                                               >  1:442439/1‑251 (MQ=255)                                                                                                                                                                       ccGAAATCTTCGTCTTCCACTTTTTTCAGCGTCTTCTCGATCTTTTTGATCAGCTTACGCTCGCGATCGCGGTTACGTAGTTCGAGGCTGAACTCTTCTTCCTGGGCTG‑CACGGTCTACCGGGTCCGGGAAGTTGGCTGCTTCATCCTGCATATGTGTAACGGTGCGATCGACTTCATCCCTGAGTTGATTACGCCATGCTTCCAGAATACGACGGAAGTGCGCCAGCTGGGCTTCATTCATATACTCTTc                                                                          >  1:376554/1‑251 (MQ=255)                                                                                                                                                                       ccGAAATCTTCGTCTTCCACTTTTTTCAGCGTCTTCTCGATCTTTTTGATCAGCTTACGCTCGCGATCGCGGTTACGTAGTTCGAGGCTGAACTCTTCTTCCTGGGCTG‑CACGGTCTACCGGGTCCGGGAAGTTGGCTGCTTCATCCTGCATATGTGTAACGGTGCGATCGACTTCATCCCTGAGTTGATTACGCCATGCTTCCAGAATACGACGGAAGTGCGCCAGCTGGGCTTCATTCATATACTCTTc                                                                          <  1:604922/251‑1 (MQ=255)                                                                                                                                                                           aaTCTTCGTCTTCCACTTTTTTCAGCGTCTTCTCGATCTTTTTGATCAGCTTACGCTCGCGATCGCGGTTACGTAGTTCGAGGCTGAACTCTTCTTCCTGGGCTG‑CACGGTCTACCGGGTCCGGGAAGTTGGCTGCTTCATCCTGCATATGTGTAACGGTGCGATCGACTTCATCCCTGAGTTGATTACGCCATGCTTCCAGAATACGACGGAAGTGCGCCAGCTGGGCTTCATTCATATACTCTTCGccc                                                                      >  1:275050/1‑251 (MQ=255)                                                                                                                                                                            aTCTTCGTCTTCCACTTTTTTCAGCGTCTTCTCGATCTTTTTGATCAGCTTACGCTCGCGATCGCGGTTACGTAGTTCGAGGCTGAAATCTTCTTCCTGGGCTG‑CACGGTCTACCGGGTCCGGGAAGTTGGCTGCTTCATCCTGCATATGTGTAACGGTGCGATCGCCTTCATCCCTGAGTTGATTACGCCATGCTTCCAGAATACGACGGAAGTGCGCCAGCTGGGCTTCATTCATATACTCTTCGCCCg                                                                     >  1:912936/1‑251 (MQ=255)                                                                                                                                                                                 cggcTTCCACTTTTTTCAGCGTCTTCTCGATCTTTTTGATCAGCTTACGCTCGCGATCGCGGTTACGTAGTTCGAGGCTGAACTCTTCTTCCTGGGCTG‑CACGGTCTACCGGGTCCGGGAAGTTGGCTGCTTCATCCTGCATATGTGTAACGGTGCGATCGACTTCATCCCTGAGTTGATTACGCCATGCTTCCAGAATACGACGGAAGTGCGCCAGCTGGGCTTCATTCATATACTCTTCGCCCGGCtt                                                                 <  2:670746/247‑1 (MQ=255)                                                                                                                                                                                  gTCTTCCCCTTTTTTCAGCGTCTTCTCGATCGTTTTGATCAGCTTACGCTCGCGATCGCGGTTACGTAGTTCGAGGCTGAACTCTTCTTCCTGGGCTG‑CACGGTCTACCGGGTCCGGGAAGTTGGCTGCTTCATCCTGCATATGTGTAACGGTGCGATCGACTTCATCCCTGAGTTGATTACGCCATGCTTCCAGAATACGACGGAAGTGCGCCAGCTGGGCTTCATTCATATACTCTTCGCCCGGCTtct                                                               <  2:684023/251‑1 (MQ=255)                                                                                                                                                                                       ccACTTTTTTCAGCGTCTTCTCGATCTTTTTGATCAGCTTACGCTCGCGATCGCGGTTACGTAGTTCGAGGCTGAACTCTTCTTCCTGGGCTG‑CACGGTCTACCGGGTCCGGGAAGTTGGCTGCTTCATCCTGCATATGTGTAACGGTGCGATCGACTTCATCCCTGAGTTGATTACGCCATGCTTCCAGAATACGACGGAAGTGCGCCAGCTGGGCTTCATTCATATACTCTTCGCCCGGCTTCTCCTg                                                           <  2:878238/250‑1 (MQ=255)                                                                                                                                                                                              tttCAGCGTCTTCGCGATCTTTTGGATCAGCTTACTCTCGCGATCGCGGTTACGTAGTGTGAGGTTGAACTCTTCTTCCTGGGCTG‑CACGGTCTACCGGGTCCGGGAAGTTGGCTGCTTCATCCTGCATATGTGTAACGGTGCGATCTACTTCATCCCTGAGTTGATTACGCCATGCTTCTAGAATACGACGGAAGTGCGCCAGCTGGGCTTCATTCATATACTCTTCGCCCGGCTTCTCCTGATATGGt                                                    <  1:898340/250‑1 (MQ=255)                                                                                                                                                                                                 cAGCGTCTTCTCGATCTTTTTGATCAGCTTACGCTCGCGATCGCGGTTACGTAGTTCGAGGCTGAACTCTTCTTCCTGGGCTG‑CACGGTCTACCGGGTCCGGGAAGTTGGCTGCTTCATCCTGCATATGTGTAACGGTGCGATCGACTTCATCCCTGAGTTGATTACGCCATGCTTCCAGAATACGACGGAAGTGCGCCAGCTGGGCTTCATTCATATACTCTTCGCCCGGCTTCTCCTGATATGGTTcc                                                 >  2:57540/1‑250 (MQ=255)                                                                                                                                                                                                 cAGCGTCTTCTCGATCTTTTTGATCAGCTTACGCTCGCGATCGCGGTTACGTAGTTCGAGGCTGAACTCTTCTTCCTATGCTG‑CACGGTCTACCGGGTCCGGGAAGTTGGCTGCTTCATCCTGCATATGTGTAACGGCGCGATCGACTTCATCCCTGAGTTGGTTACGCCATGCTTCCAGAATACGACGGAAGTGCGCCAGCTGGGCTTCATTCATATACTCTTCGCCCGGCTTCTCCTGATATGGTTccc                                                >  1:398205/1‑250 (MQ=255)                                                                                                                                                                                                  aGCGTCTTCTCGATCATTTTGATCAGCTTACGCTCGCGATCGCGGTTACGTAGGTCGAGGCTGAACTCTTCTTCCTGGGCTG‑CACGGTCTACCGGGTCCGTGAAGTTTGCTGCTTCATCCTGCAGATGTGTAACGGGGCGATCGACTTCATCCCTGAGTTGATTACGCCATGCTTCCAGAATACGACGGAAGTGCGCCAGCTGGGCTTCATTCATATACTCTTCTCCCGGCTTCTCCTGATATGGTTCCAc                                               <  2:804618/251‑1 (MQ=255)                                                                                                                                                                                                            cGATCTTTTTGATCAGTCTACGCTCGCGATCGCGGTTACGTAGTTCGAGGCTGAACTCTTCTTCCTGGGCTG‑CACGGTCTACCGGGTCCGGGAAGTTGGCTGCTTCATCCTGCATATGTGTAACGGTGCGATCGACTTCATCCCTGAGTTGATTACGCCATGCTTCCAGAATACGACGGAAGTGCGCCAGCTGGGCTTCATTCATATACTCTTCGCCCGGCTTCTCCTGATATGGTTCCACCCCAGCg                                        <  2:234726/248‑1 (MQ=255)                                                                                                                                                                                                                  ttttGATCAGCTTACGCTCGCGATCGCGGTTACGTAGTTCGAGGCTGAACTCTTCTTCCTGGGCTG‑CACGGTCTACCGGGTCCGGGAAGTTGGCTGCTTCATCCTGCATATGTGTAACGGTGCGATCGACTTCATCCCTGAGTTGATTACGCCATGCTTCCAGAATACGACGGAAGTGCGCCAGCTGGGCTTCATTCATATACTCTTCGCCCGGCTTCTCCTGATATGGTTCCACCCCAGCGATGGCgag                                <  1:45854/250‑1 (MQ=255)                                                                                                                                                                                                                  ttttGATCAGCTTACGCTCGCGATCGCGGTTACGTAGTTCGAGGCTGAACTCTTCTTCCTGGGCTG‑CACGGTCTACCGGGTCCGGGAAGTTGGCTGCTTCATCCTGCATATGTGTAACGGTGCGATCGACTTCATCCCTGAGTTGATTACGCCATGCTTCCAGAATACGACGGAAGTGCGCCAGCTGGGCTTCATCCATATACTCTTCGCCCGGCTTCTCCTGATATGGTTCCACCCCAGCGATGGCga                                 <  1:565380/249‑1 (MQ=255)                                                                                                                                                                                                                   tttGATCAGCTTACGCTCGCGATCGCGGTTACGTAGTTCGAGGCTGAACTCTTCTTCCTGGGCTG‑CACGGTCTACCGGGTCCGGGAAGTTGGCTGCTTCATCCTGCATATGTGTAACGGTGCGATCGACTTCATCCCTGAGTTGATTACGCCATGCTTCCAGAATACGACGGAAGTGCGCCAGCTGGGCTTCATTCATATACTCTTCGCCCGGCTTCTCCTGATATGGTTCCACCCCAGCGATGGCgcga                               >  2:484560/1‑247 (MQ=255)                                                                                                                                                                                                                       aTCAGCTTACGCTCGCGATCGCGGTTACGTAGTTCGAGGCTGAACTCTTCTTCCTGGGCTG‑CACGGTCTACCGGGTCCGGGAAGTTGGCTGCTTCATCCTGCATATGTGTAACGGTGCGATCGACTTCATCCCTGCGTTGATTACGCCATGCTTCCAGAATACGACGGAAGTGCGCCAGCTGGGCTTCATTCATATACTCTTCGCCCGGCTTCTCCTGATATGGTTCCACCCCAGCGATGGCGAGCATa                            >  2:844992/1‑249 (MQ=255)                                                                                                                                                                                                                       aTCAGCTTACGCTCGCGATCGCGGTTACGTAGTTCGAGGCTGAACTCTTCTTCCTGGGCTG‑CACGGTCTACCGGGTCCGGGAAGTTGGCTGCTTCATCCTGCATATGTGTAACGGTGCGATCGACTTCATCCCTGAGTTGATTACGCCATGCTTCCAGAATACGACGGAAGTGCGCCAGCTGGGCTTCATTCATATACTCTTCGCCCGGCTTCTCCTGATATGGTTCCACCCCAGCGATGGCGAGAATa                            <  1:844992/249‑1 (MQ=255)                                                                                                                                                                                                                         cAGCTTACGCTCGCGATCGCGGTTACGTAGTTCGAGGCTGAACTCTTCTTCCTGGGCTG‑CACGGTCTACCGGGTCCGGGAAGTTGGCTGCTTCATCCTGCATATGTGTAACGGTGCGATCGACTTCATCCCTGAGTTGATTACGCCATGCTTCCAGAATACGACGGAAGTGCGCCAGCTGGGCTTCATTCATATACTCTTCGCCCGGCTTCTCCTGATATGGTTCCACCCCAGCGATGGCGAGAATACTc                         <  1:301398/250‑1 (MQ=255)                                                                                                                                                                                                                             ttACGCTCGCGATCGCGGTTACGTAGTTCGAGGCTGAACTCTTCTTCCTGGGCTG‑CCCGGTCTACCGGGTCCGGGAAGTTGGCTGCTTCATCCTGCATATGTGTAACGGTGCGATCGACTTCATCCCTGAGTTGATTACGCCATGCTTCCAGAATACGACGGAAGTGCGCCAGCTGGGCTTCATTCATATACTCTTCGCCCGGCTTCTCCTGATATGGTTCCACCCCAGCCATGGCGAGGATACACAggg                     >  2:156643/1‑250 (MQ=255)                                                                                                                                                                                                                             ttACGCTCGCGATCGCGGTTACGTAGTTCGAGGCTGAACTCTTCTTCCTGGGCTG‑CACGGTCTACCGGGTCCGGGAAGTTGGCTGCTTCATCCTGCATATGTGTAACGGTGCGATCGACTTCATCCCTGAGTTGATTACGCCATGCTTCCAGAATACGACGGAAGTGCGCCAGCTGGGCTTCATTCATATACTCTTCGCCCGGCTTCTCCTGATATGGTTCCACCCCAGCGATGGCGAGAATACTCAggg                     >  2:891039/1‑250 (MQ=255)                                                                                                                                                                                                                              tACGCTCGCGATCGCGGTTACGTAGTTCGAGGCTGAACTCTTCTTCCTGGGCTG‑CACGGTCTACCGGGTCCGGGAAGTTGGCTGCTTCATCCTGCATATGTGTAACGGTGCGATCGACTTCATCCCTGAGTTGATTACGCCATGCTTCCAGAATACGACGGAAGTGCGCCAGCTGGGCTTCATTCATATACTCTTCGCCCGGCTTCTCCTGATATGGATCCACCCCAGCGATGGCGGGAATCCTCAGGGAc                   >  2:544105/1‑251 (MQ=255)                                                                                                                                                                                                                                       gATCGCGGTTACGTAGTTCGAGGCTGAACTCTTCTTCCTGGGCTG‑CACGGTCTACCGGGTCCGGGAAGTTGGCTGCTTCATCCTGCATATGTGTAACGGTGCGATCGACTTCATCCCTGAGTTGATTACGCCATGCTTCCAGAATACGACGGAAGTGCGCCAGCTGGGCTTCATTCATATACTCTTCGCCCGGCTTCTCCTGATATGGTTCCACCCCAGCGATGGCGAGAATACTCAGGGACGATGtttt           >  1:568843/1‑250 (MQ=255)                                                                                                                                                                                                                                             ggTTACGTAGTTCGAGGCTGAACTCTTCTTCCTGGGCTG‑CACGGTCTACCGGGTCCGGGAAGTTGGCTGCTTCATCCTGCATATGTGTAACGGTGCGATCGACTTCATCCCTGAGTTGATTACGCCATGCTTCCAGAATACGACGGAAGTGCGCCAGCTGGGCTTCATTCATATACTCTTCGCCCGGCTTCTCCTGATATGGTTCCACCCCAGCGATGGCGAGAATACTCAGGGACGATGTTTTACGGttt    <  1:939331/251‑1 (MQ=255)                                                                                                                                                                                                                                                tACGTAGTTCGAGGCTGAACTCTTCTTCCTGGGCTG‑CACGGTCTACCGGGTCCGGGAAGTTGGCTGCTTCATCCTGCATATGTGTAACGGTGCGATCGACTTCATCCCTGAGTTGATTACGCCATGCTTCCAGAATACGACGGAAGTGCGCCAGCTGGGCTTCATTCATATACTCTTCGCCCGGCTTCTCCTGATATGGTTCCACCCCAGCGATGGCGAGAATACTCAGGGACGATGTTTTACGGTTTTg  <  1:205507/250‑1 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                       CGGGAGCATTTCCCGCCTGTGGTAAACGTGATGGAACGGCTGTAATTAGCCAGCCATCTGTTTTTCGCGAATTTCAGCCAGCGTTTTGCAGTCGATGCACAGATCGGCTGTCGGGCGCGCTTCCAGACGGCGAATACCAATTTCAACACCGCAGGATTCGCAGTAGCCGAAATCTTCGTCTTCCACTTTTTTCAGCGTCTTCTCGATCTTTTTGATCAGCTTACGCTCGCGATCGCGGTTACGCAGTTCGAGGCTGAACTCTTCTTCCTGGGCTG‑CACGGTCTACCGGGTCCGGGAAGTTGGCTGCTTCATCCTGCATATGTGTAACGGTGCGATCGACTTCATCCCTGAGTTGATTACGCCATGCTTCCAGAATACGACGGAAGTGCGCCAGCTGGGCTTCATTCATATACTCTTCGCCCGGCTTCTCCTGATATGGTTCCACCCCAGCGATGGCGAGAATACTCAGGGACGATGTTTTACGGTTTTG  >  NC_000913/160104‑160592

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑