| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 92,843 | A→G | P477P (CCA→CCG) | ftsI → | peptidoglycan DD‑transpeptidase FtsI |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 92,843 | 0 | A | G | 97.2% | 323.9 / ‑2.4 | 106 | P477P (CCA→CCG) | ftsI | peptidoglycan DD‑transpeptidase FtsI |
| Reads supporting (aligned to +/- strand): ref base A (2/1); new base G (48/55); total (50/56) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.01e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.98e-01 | |||||||||||
Only 100 of 106 total aligned reads displayed.
AGAACGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAACACCATTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCGGTGAACGTGTCTTCCCGGAATCCATTGTCCGC‑ACTGTGGTGCATATGATGGAAAGCGTGGCGCTACCAGGCGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCGGTACCGCGAAAAAGGTCGGGCCGGACGGTCGCTACATCAATAAATATATTGCTTATACC‑GCAGGCGTTGCGCCTGCGAGTCAGCCGCGCTTCGCGCTGGTTGTTGTTATCAACGATCCGCAGGCGGGTAAATACTACGGCGGCGCC‑G‑TTTCCG‑CGCCGGTCTTTGGTGCCATCATGGG > NC_000913/92597‑93076 | agaACGCAGTGGTTTATATCCTCAAAAACAACGGTGTTCTGACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAACACCGTTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCGGTGAACGTGTCTTCCCGGAATCCATTGTCCGC‑ACCGTGGTGCATATGATGGAAAGCGTGGCGCTACCGggcg < 2:81119/251‑1 (MQ=255)agaACGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGCGAGGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAACACCGTTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCGGTGAACGTGTCTTCCCGGAATCCATTGTCCGCCAC‑GTGGTGCATATGATGGCAAGCGTGGCGCTACCGggcg > 2:421512/1‑251 (MQ=255) gCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAACACCGTTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCGGTGAACGTGTCTTCCCGGAATCCATTGTCCGC‑ACCGTGGTGCATATGATGGAAAGCGTGGCGCTACCGggcggcggc > 1:633007/1‑251 (MQ=255) cAGTGGCTTATATCCTCAAAAAAAACGGTGGTCTGACATAGAGAGGGCCAACTTCTCTTTCGGCTACGGGCTAATGGTAACACCGTTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGCTTACCAAAGTTGACCCCACGGTTCCCGGTGAACGTGTCTTCCCGGAATCCATTGTCCGC‑ACCGTGGTGCATCTGATGGCAAGCGTGGCGCTCCCGggcggcggcg > 2:629337/1‑251 (MQ=255) tGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAACACCGTTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCGGTGAACGTGTCTTCCCGGACTCCATTGTCCGC‑ACCGTGGTGCATATGATGGAAAGCGTGGCGCTACCGGgcggcggcggcg > 1:363615/1‑251 (MQ=255) atatCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAACACCGTTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCGGTGAACGTGTCTTCCCGGAATCCATTGTCCGC‑ACCGTGGTGCATATGATGGAAAGCGTGGCGCTACCGGGCGGCGGCGGCGTGAAgg > 1:447551/1‑251 (MQ=255) tatCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAACACCGTTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCGGTGAACGTGTCTTCCCGGAATCCATTGTCCGC‑ACCGTGGTGCATATGATGGAAAGCGTGGCGCTACCGGGCGGCGGCGGCGTGAAggc > 2:138551/1‑251 (MQ=255) cAAAAACAACGGTGGTCTGACATAGAGATGGCCTCCTTCTCTTTCGGCTACGGGCTAATGGTAACACCGTTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCGGTGAACGTGTCTTCCCGGAATCCATTGTCCGC‑ACCGTGGTGCATATGATGGAAAGCGTGGCGCTACCGGGCGGCGGCGGCGTGAAGgcggcg < 2:408727/249‑1 (MQ=255) aaaaCAACGGTGGTCTGACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAACACCGTTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCGGTGAACGTGTCTTCCCGGAATCCATTGTCCGC‑ACCGTGGTGCATATGATGGAAAGCGTGGCGCTACCGGGCGGCGGCGGCGTGAAGGCGGCGATTa < 1:116533/251‑1 (MQ=255) acaacGGTGGTCTGACATATAGAGGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAACACCGTTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCGGTAAACGTGGCTTCCCGGAATCCATTGTCCGC‑ACCGGGGTGCATCTGAAGGAAAGCGTGGCGCTACCGGGCGGCGGCGGCGGGAGGGCGGCGATTaa > 2:580081/1‑249 (MQ=255) acaacGGTGGTCTGACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAACACCGTTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCGGTGAACGTGTCTTCCCGGAATCCATTGTCCGC‑ACCGTGGTGCATATGATGGAAAGCGTGGCGCTACCGGGCGGCGGCGGCGTGAAGGCGGCGATTaa > 1:439644/1‑249 (MQ=255) cGGTGGTCTGACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAACACCGTTACAGTTAGCGCGAGTCTACGCAACTATCCGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCGGTGAACGTGTCTTCCCGGAATCCATTGTCCGC‑ACCGTGGTGCATATGATGGAAAGCGTGGCGCTACCGGGCGGCGGCGGCGTGAAGGCGGTGATTAAAGGCta < 2:602491/251‑1 (MQ=255) gtCTGACATAGAGAGGGCCACCTTCTCTTGCGGCTACGGGCTAATGGTAACACCGTTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCGGTGAACGTGTCTTCCCGGAATCCATTGTCCGC‑ACCGTGGTGCATATGATGGAAAGCGTGGCGCTACCGGGCGGCGGCGGCGTGAAGGCGGCGATTAAAGGCtatcgta < 2:777695/251‑1 (MQ=255) tCTGACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAACACCGTTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCGGTGAACGTGTCTTCCCGGAATCCATTGTCCGC‑ACCGTGGTGCATATGATGGAAAGCGTGGCGCTACCGGGCGGCGGCGGCGTGAAGGCGGCGATTAAAGGCtatcgtat > 1:472462/1‑251 (MQ=255) tGACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAACACCGTTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCGGTGAACGTGTCTTCCCGGAATCCATTGTCCGC‑ACCGTGGTGCATATGATGGAAAGCGTGGCGCTACCGGGCGGCGGCGGCGTGAAGGCGGCGATTAAAGGCtatcgtatcg > 1:645819/1‑251 (MQ=255) gACATAGAGAGGGCCACCTTCTCTTTCGTCTACGGGCTAATGGTAACACCGTTACAGTTTGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCGGTGAACGTGTCTTGCCGGAATCCATTGTCCGC‑ACCGTGGTGCATATGATGGAAAGCGTGGCGCTACCGGGCAGCGGCGGCGTGAAGGCTGCGATTAAAGGCtatcgtatc < 1:987876/249‑1 (MQ=255) gACATAGAGAGGGCCACCCTCTCTTTCGGCTACGGGCTAATGGTAACACCGTTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCGGTGAACGTGTCTTCCCGGAATCCATTGTCCGC‑ACCGTGGTGCATATGATGGAAAGCGTGGCGCTACCGGGCGGCGGCGGCGTGAAGGCGGCGATTAAAGGCtatcgtatcg < 2:309442/250‑1 (MQ=255) agagagGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAACACCGTTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCGGTGAACGTGTCTTCCCGGAATCCCTTGTCCGC‑ACCGTGGTGCATATGATGGAAAGCGTGGCGCTACCGGGCGGCGGCGGCGTGACGGCGGCGGTTAAAGGCTATCGTATCGCCAtt > 1:249780/1‑250 (MQ=255) gatagGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAACACCGTTACAGTTAGCGCGAGTCTACGCAACTCTCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCGGTGAACGTGTCTTCCCGGAATCCATTGTCCGC‑ACCGTGGAGCAACTGATGGAAAGCGTGGCGCTACCGGGCAGCGGCGGAGTGAAGGCGGCGATTAAAGGCTCTCGTATCGcccttc > 2:616154/4‑246 (MQ=255) gagagGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAACACCATTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCGGTGAACGTGTCTTCCCGGAATCCATTGTCCGC‑ACTGTGGTGCATATGATGGAAAGCGTGGCGCTACCAGGCGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTaa < 1:267491/251‑1 (MQ=255) agagGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAACACCATTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCGGTGAACGTGTCTTCCCGGAATCCATTGTCCGC‑ACTGTGGTGCATATGATGGAAAGCGTGGCGCTACCAGGCGGCGGCGGCGTGAAGGAGGCGATTAAAGGCTATCGTATCGCCATTaa > 1:134170/1‑250 (MQ=255) agagGGCCACCTTCTCTTTCGGCTACGAGCTAATGGTAACACCGTTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCACGGTTCCCGGTGAACGTGTCTTCCCGGAATCCATTGTCCGC‑ACCGTGGTGCATATGATGGAAAGCGTGGCGCTACCGGGCGGCGGCGGCGTGAAGGAGGCGATTAAAGGATATCGTATCGCCATTaa > 2:884133/1‑250 (MQ=255) gagGGCCACCTTCCCTTTCGGCTACGGGCTAATGGTAACACCGTTACAGTTAGCGCGAGTCTTCGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCTCCCGGTTCCCGGTGAACGTGTCTTCCCGGAATCCATTGTCCGC‑TCCGTGGTGCATATGATGGAAAGCGTGGCGCTACCGGGCGGCGGCGGCGTGAAGGCTGCGATTAAAGGCTATCGTATCGCCATTaaaa < 1:824799/251‑1 (MQ=255) gacggCCACCTTCTCTTTCGGCTACGGGCTAATGGTAACACCGTTACAGTGAGTGCGAGTCTACGCAACTATCGGCAGCTAAGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCGGTGAACGTGTCTTCCCGGAATCCATTGTCCGC‑ACCGTGGTGCATATGATTGAAAGCGTGGCGCTTCCGGGCGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTaaaa < 2:429801/248‑1 (MQ=255) agGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAACACCGTTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCGGTGAACGTGTCTTCCCGGAATCCATTGTCCGC‑ACCGTGGTGCATATGATGGAAAGCGTGGCGCTACCGGGCGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAAc > 2:908763/1‑251 (MQ=255) gCCACCTTCTCTTTCGGCTACGGGCTAATGGTAACACCGTTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCGGTGAACGTGTCTTCCCGGAATCCATTGTCCGC‑ACCGTGGTGCATATGATGGAAAGCGTGGCGCTACCGGGCTGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCgg > 1:2654/1‑251 (MQ=255) accACCTTCTCTTTCGGCTACGGGCTAATGGTAACACCGTTACAGTTCGCGCGCGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCGGTGAACGTGTCTTCCCGGAATCCATTGTCCGC‑ACAGTGGTGCATATGATGGAAAGCGTGGCGCTACCGTGCGGCGGCGGCGGGAAGGGGGCGATTAAGGGCGATCGTATGCCCATTAAAACCgg > 2:777898/2‑251 (MQ=255) cACCTTCTCTTTCGGCTACGGGCTAATGGTAACACCGTTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCGGTGAACGTGTCTTCCCGGAATCCATTGTCCGC‑ACCGTGGTGCATATGATGGAAAGCGTGGCGCTACCGGGCGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCGGt > 1:297845/1‑250 (MQ=255) aaTGGTAACACCGTTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCGGTGAACGTGTCTTCCCGGAATCCATTGTCCGC‑ACCGTGGTGCATATGATGGAAAGCGTGGCGCTACCGGGCGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCGGTACCGCGAAAAAGGTCGGGCCGGACg < 1:361111/251‑1 (MQ=255) aTGGTAACACCGTTACAGTTAGCGCAAGTCTAAGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGGCGATTACCAAAGTTGACCCCCCGGTTCCCGGGGAACGTGTCTTCCCGGAATCCATTGTCCGC‑ACCGTGGTGCATATGATGGAAAGCGTGGCGCTACCGGGCGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCGGTACCGCGAAAAAGGTCGGGCCGGACgg < 2:501399/251‑1 (MQ=255) tGGTAACACCGTTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCGGTGAACGTGTCTTCCCGGAATCCATTGTCCGC‑ACCGTGGTGCATATGATGGAAAGCGTGGCGCTACCGGGCGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCGTTAACACCGGGCCAGCGAAATAGGTCGGGCCGGAAGGt > 2:679091/1‑251 (MQ=255) tGGTAACACCGTTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCGGTGAACGTGTCTTCCCGGAATCCATTGTCCGC‑ACCGTGGTGCATATGATGGAAAGCGTGGCGCTACCGGGCGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCGGTACCGCGAAAAAGGTCGGGCCGGACGGt > 1:322035/1‑251 (MQ=255) gggTAACACCGTTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCAGTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCGGTGAACGTGTCTGTCCGGAATCCATTGTCCGC‑ACCGTGGTGCATATGATGGAAAGCGTGGCGCTACCGGGAGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCGTTACCGCGAAAAAGGTCGGGCCGGACGGt < 1:231251/250‑1 (MQ=255) ccgttACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCGGTGAACGTGTCTTCCCGGAATCCATTGTCCGC‑ACCGTGGTGCATATGATGGAAAGCGTGGCGCTACCGGGCGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCGGTACCGCGAAAAAGGTCGGGCCGGACGGTCGCTACAt < 1:766485/248‑1 (MQ=255) cgttACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCGGTGAACGTGTCTTCCCGGAATCCATTGTCCGC‑ACCGTGGTGCATATGATGGAAAGCGTGGCGCTACCGGGCGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCGGTACCGCGAAAAAGGTCGGGCCGGACGGTCGCTACATc < 1:631198/249‑1 (MQ=255) tACAGTTAGCGGGAGTCTACGCAACTATCGTCAGCTACGGCATTTACCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCGGTGAACGTGTCTTCCCGGAATCCATTGTCCGC‑ACCGTGGTGCATATGATGGAAAGCGTGGCGCTACCGGGCGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCGTTACCGCGAAAAAGGTCGGGCCGGACGGTCGCTACATCaa < 2:633007/250‑1 (MQ=255) aCAGTGAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCGGTGAACGTGTCTTCCCGGAATCCATTGTCCGC‑ACCGTGGTGCATATGATGGAAAGCGTGGCGCTACCGGGCGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCGGTACCGCGAAAAAGGTCGGGCCGGACGGTCGCTACATCaat < 2:94318/250‑1 (MQ=255) cAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCGGTGAACGTGTCTTCCCGGAATCCATTGTCCGC‑ACCGTGGTGCATATGATGGAAAGCGTGGCGCTACCGGGCGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCGGTACCGCGAAAAAGGTCGGGCCGGACGGTCGCTACATCaataa < 1:715021/251‑1 (MQ=255) aGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCGGTGAACGTGTCTTCCCGGAATCCATTGTCCGC‑ACCGTGGTGCATATGATGGAAAGCGTGGCGCTACCGGGCGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTACAACCGGTACCGCGAAAA‑GGTCGGGCCGGACGGACGCTACATCaataaat > 1:956830/1‑251 (MQ=255) aGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCGGTGAACGTGTCTTCCCGGAATCCATTGTCCGC‑ACCGTGGTGCATATGATGGAAAGCGTGGCGCTACCGGGCGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCGGTACCGCGAAAAAGGTCGGGCCGGACGGTCGCTACATCaataa > 1:687896/1‑250 (MQ=255) tAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCGGTGAACGTGTCTTCCCGGAATCCATTGTCCGC‑ACCGTGGTGCATATGATGGAAAGCGTGGCGCTACCGGGCGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCGGTACCGCGAAAAAGGTCGGGCCGGACGGTCGCTACATCaataaata < 1:597744/250‑1 (MQ=255) tAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCAGGTGAACGTGTCTTCCCGGAATCCATTGTCCGC‑ACCGTGGTGCATATGATGGAAAGCGTGGCGCTACCGGGCGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCGGTACCGCGAAAAAGGTCGGGCCGGACGGTCGCTACATCaataaata < 1:224950/250‑1 (MQ=255) aGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCGGTGAACGTGTCTTCCCGGAATCCATTGTCCGC‑ACCGTGGTGCATATGATGGAAAGCGTGGCGCTACCGGGCGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCGGAACCGCGAAAAAGGTCGGGCCGGACGGTCGCTACATCAATAAatat > 2:51540/1‑250 (MQ=255) cgAGTCTCCGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGCCCCCCCGGTTCCCGGTGAACGTGTCTTCCCGGAATCCATTGTCCGC‑ACCGTGGTGCATATGATGGAAAGCGTGGCGCTACCGGGCGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCGGTACCGCGAAAAAGGTCGGGCCGGACGGTCGCTACATCAATAAATATATTGc < 2:258693/251‑1 (MQ=255) gTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCGGTGAACGTGTCTTCCCGGAATCCATTGTCCGC‑ACCGTGGCGCATCTGATGGAAAGCGTGGCGCTACCAGGAGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATAGTATCGCCATTAAAACCGGTACCGCGAAAAAGGAAGGGACGGACGGGCGCAACATCaataaaaaaattgtttt > 2:393466/1‑240 (MQ=255) tACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCGGTGACCGTGTCTTCCCGGAATCCATTGTCCGC‑ACCGTGGTGCATATGCTAGAAAGCGAGGCGCTACCGGGCGGCGGAGGCGTGCAGGCGGCGATTAAAGGCTATCGTATCTCCATTCAAACCGGTACCGCGAAAAAGGTCGGGCCGGACGGTCTCTACATCACTAACTATATTGCTTATAc > 2:946890/1‑251 (MQ=255) aCGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCGGTGAACGTGTCTTCCCGGAATCCATTGTCCGC‑ACCGTGGTGCATATGATGGAAAGCGTGGCGCTACCGGGCGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCTGTACCGCGAAAAATGTCGGGCCGGACGGTCGCTACATCAATAAATATATTGCTtat < 1:386643/248‑1 (MQ=255) cacatatcgtcatgtccgtcctttATCGCCCAATGTCGAGTACCAAAGTTGACCCCCCGGTTCCCGGTGAACGTGTCTTCCCGGAATCCATTGTCCGC‑TCCGTGTTGCATATGATGGAAAGCGTGGCGCTACCGGGCGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCGGTACCGCGAAAAAGGTCGGGCCGGACGGTCGCTACATCAATAAATATATTGCTTATACC‑GCa < 2:896791/230‑1 (MQ=255) tATCGGCAGCTACGGCATTTATCGCCCACTGTCGGTTACCAAAGTTGACCCCCCGGTTCCCGGTGAACGTGTCTTCCCGGAATCCATTGTCCGC‑ACCGTGGTGCATATGATGGAAAGCGTGGCGCTACCGGGCGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATAGCCATTAAAACCGGTACCGCGAAAAAGGTCGGCCCGGACGGTCGCACCATCAATAAATATATTGCTTATACC‑GCAGGCg > 2:939550/1‑251 (MQ=255) cGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCGGTGAACGTGTCTTCCCGGAATCCATTGTCCGC‑ACCGTGGTGCATATGATGGAGAGCGTGGCGCTACCGGGCGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCGGTACCGCGAAAAAGGTCGGGCCGGACGGTCGCTACATCAATAAATATATTGCTTATACC‑GCAGGCGtt < 1:900057/250‑1 (MQ=255) cGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCGGTGAACGTGTCTTCCCGGAATCCATTGTCCGC‑ACCGTGGTGCATATGATGGAAAGCGTGGCGCTACCGGGCGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCGGTACCGCGAAAAAGGTCGGGCCGGACGGTCGCTACATCAATAAATATATTGCTTATACC‑GCAGGCGTTg < 1:460968/251‑1 (MQ=255) gCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCGGTGAACGTGTCTTCCCGGAATCCATTGTCCGC‑ACCGTGGTGCATATGATGGAAAGCGTGGCGCTACCGGGCGGCGGCGGCGGGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCGGTACCGCGAAACAGGTCGGGCCGGACGGTCGCTACCTCAATAAATATATTGCTTATACC‑GCAGGCGTTgcg > 2:106396/1‑251 (MQ=255) cAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCGGTGAACGTGTCTTCCCGGAATCCATTGTCCGC‑ACCGTGGTGCATATGATGGAAAGCGTGGCGCTACCGGGCGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCGGTACCGCGAAAAAGGTCGGGCCGGACGGTCGCTACATCAATAAATATATTGCTTATACC‑GCAGGCGTTgcgc > 1:513631/1‑251 (MQ=255) ccGGCATTTACCGCCGACTGTCTATTACCAAAGTTGACCCCCCGGTTCCCGGGGAACGTGGCTTCCCGGAATCCATTGTCCGC‑GCCGTGGTGCATATGAGGGAAAGCGTGGCGCTACCGGGCGGCGGCGGCGTGAAGGCGTCGATTAAAGGCTAGCGTATCGCCATTAAAACCGGTACCGCGAAAAAGGGCGGGCCGGTCGGGCGCTACATCAATAAATAGATGGCTTATACC‑GCATGCGTTGCGCCTGCg < 2:95404/250‑1 (MQ=255) cGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCGGTGAACGTGTCTTCCCAGAATCCATTGTCCGC‑ACCGTGGTGCATATGATGGAAAGCGTGGCGCTACCGGGCGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCGGTACCGCGAAACAGGTCGGGCCGGACGGCCGCTACATCAATAACAATATTGCTTATACC‑GCCGGCGTTGCGCCTGCgg > 2:973405/1‑250 (MQ=255) atcgcacacttgcgagtACCAAAGTTGACCCCCCGGTTCCCGTTGAACGTGTCTTCCCGGAATCCATTGTCCGC‑ACCGTGGGTCATATGATGTAAAGCGGGGCGCTACCGGGCGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATTGTATCGCCATTAAAACCGGTACCGCGAAAAAGGTCGGGCCGGACGGTCGCTACATCAATAAATATATTGCTTATACC‑GCAGGCGTTGCGCCTGCGAGTCAGCcg < 2:105349/235‑1 (MQ=255) aacccccACTGTCGAGTACCAAAGTTGACCCCCCGGTTCCCGGGGAACGTGTCTTCCCGGAATCCATTGTCCGC‑ACCGTGGTGCATATGATGGAAAGCGTGGCGCTACCGGGCGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCGGTACCGCGAAAAAGGTCGGGCCGGACGGTCGCTACATCAATAAATATATTGCTTATACC‑GCAGGCGTTGCGCCTGCGAGTCAGcc < 2:31770/246‑1 (MQ=255) aTCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCGGTGAACGTGTCTTCCCGGAATCCATTGTCCGC‑ACCGTGGTGCATATGATGGAAAGCGTGGCGCTACCGGGCGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCGGTACCGCGAAAAAGGTCGGGCCGGACGGTCGCTACATCAATAAATATATTGCTTATACC‑GCAGGCGTTGCGCCTGCGCGTCAGCcg > 2:269338/1‑251 (MQ=255) aTCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCGGTGAACGTGTCTTCCCGGAATCCATTGTCAGC‑ACCGTGGTGCATATGATGGAAAGCGTGGCGCTACCGGGCGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCGGTACCGCGAACAAGGTCGGGCCGGACGGTCGCTACATCACTAAATATATTGCTTATACC‑GCAGGCGGTGCGCCTGCGAGTCAGCcg > 2:67260/1‑251 (MQ=255) ccccTGTCGATTACCAAAGTGGACCCCCCGGTTCCCGGTGAACGTGTCTTCCCGGAATCCATTGTCCGC‑ACCGTGGTGCATATGATGGAAAGCGTGGCGCTACCGGGCGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCGGTACCGCGAAAAAGGTCGGGCCGGACGGTCGCTACATCAATAAATATATTGCTTATACC‑GCAGGCGTTGCGCCTGCGAGTCAGCCGCGCtt < 2:353265/248‑1 (MQ=255) ccTGTCGATTACCAAAGTTGACCCCCCGGTTCCCGGTGAACGTGTCTTCCCGGAATCCATTGTCCAC‑ACCGTGGTGCATATGATGGAAAGCGTGGCGCTACCGGGCGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCGGTACCGCGAAAAAGGTCGGGCCGGACGGTCGCTACATCAATAAATATATTGCTTATACC‑GCAGGCGTTGCGCCTGCGAGTCAGCCGCGCTTcg < 1:808811/250‑1 (MQ=255) gTCGATTACCAAAGTTGTCCCCCCGGTGCCCGGTGAACGTGTCTTCCCGGAATCCATTGTCCGC‑ACCGTGGTGCATATGATGGAAAGCGTGGCGCTACCGGGCGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCGGTACCGCGAAAAAGGTCGGGCCGGACGGTCGCTACATCAATAAATATATTGCTTATACC‑GCAGGCGTTGCGCCTGCGAGTCAGCCGCGCTTcgcg < 2:882161/250‑1 (MQ=255) cGATTACCAAAGGTGACCCCCCGGTTCCCGGTGAACGTGTCTTCCCGGAATCCATTGTGCGC‑ACCGTGGTGCATATGATGGAAAGCGTGGCGCTACCGGGCGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCGGTACCGCGAAAAAGGTCGGGCCGGACGGTCGCTACATCAATAAATATATTGCTTATACC‑GCAGGCGTTGCGCCTTCGAGTCAGCCGCGCTGCGCGCt < 2:921775/250‑1 (MQ=255) tACCAAAGTTGTCCCCCCGGTTCCCGGTGAACGTGTCTTCCCGGAATCCTTTGTCCTC‑CCCGTGTTGCATATGATGGAAAGCGTGGCGCTTCCGGGCGGCGGCGGCGTGAAGGCGGCGAGTAAAGGCTATCGTATCGCCATTAAAACCTGTACCGCGAAAAATGTCGGGCCGGACGGTCGCTACATCAATAAATATATTGCTTATACC‑GCAGGCGTTGCGCCTGCTAGTCTGCCGCGCTTCGCGCTGgtt < 2:711133/250‑1 (MQ=255) tACCAAAGTTGACCCCCCGGTTCCCGGTGAACGTGTCTTCCCGGAATCCATTGTCCGC‑ACCGTGGTGCATATGATGGAAAGCGTGGCGCTACCGGGCGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCGGTACCGCGAAAAAGGTCGGGCCGGACGGTCGCTACATCAATAAATATATTGCTTATACC‑GCAGGCGTTGCGCCTGCGAGTCAGCCGCGCTTCGCGCTGgtt < 1:983251/250‑1 (MQ=255) gTTGACCCCCCGGTTCCCGGTGAACGTGTCTTCCCGGAATCCATTGTCCGC‑ACCGTGGTGCATATGATGGAAAGCGTGGCGCTACCGGGCGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCGGTACCGCGAAAAAGGTCGGGCCGGACGGTCGCTACATCAATAAATATATTGCTTATACC‑GCAGGCGTTGCGCCTGCGAGTCAGCCGCGCTTCGCGCTGGTTGTTGTTAt < 1:393466/251‑1 (MQ=255) tGACCCCCCGGTTCCCGGGGAACGTTTCTTCCCGGAAGCCATTGTCCGC‑ACCGGGTTGCATATGATGGAAAGCGTGGCGTTACCGGGCGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTAGCGTATCGCCATTAAAACCGGTACCGCGAAAAAGGTCGGGCCGGACGGTCGCTACATCAATAAATATATTGCTTATACC‑GCAGGCGTTGCGCCTGCGAGTCAGCCGCGCTTCGCGCTGGTTGTTGTTATc < 2:705785/250‑1 (MQ=255) aCCCCCCGGTTCCCGGTGAACGTGTCTTCCCGGAATCCATTGTCCGC‑ACCGTGGTGCATATGATGGAAAGCGTGGCGCTACCGGGCGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCGGTACCGCGAAAAAGGTCGGGCCGGACGGTCGCTACATCAATAAATATATTGCTTATACC‑GCAGGCGTTGCGCCTGCGAGTCAGCCGCGCTTCGCGCTGGTTGTTGTTATCAAc > 1:739604/1‑251 (MQ=255) ccccGGTTCCCGGTGAACGTGTCTTCCCGGAATCCATTGTCCGC‑ACCGTGTTGCATATGATGGAAAGCGTGGCGCTACCGGGCGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCGGTACCGCGAAAAAGGTCGGGCCGGACGGTCGCTACATCAATAAATATATTGCTTATACC‑GCAGGCGTTGCGCCTGCGAGTCAGCCGCGCTTCGCGCTGGTTGTTGTTATCAACGAt > 1:586891/1‑251 (MQ=255) ccGGTCCCCGGTGAACGGGTCTTCCCGGAATCACTTGGCCGT‑ACTGTGGTGCATATGATGGAAAGCGTGGCGATACCGGGCGTCGGCGGCGTGAAGGTGGCGATTAAAGGCTATCGTATCGCCATTAAAACCGGTACCGCGAAAAAGGTCGTGCTGTACGGTCGCTACATCAATAAATATATTGCTGATTCC‑GCATGCGTTGAGCCTGCGAGGCAGCCGCGCTTCGCGCTGGTTGTTGTTATAAACGagct < 2:306520/251‑4 (MQ=255) gTTCACGGTGAACGTGGCTTCCCGGAATCCTTTATCCGC‑ACCGTGGTGCATATGATGGAAAGCGCGGCGCTACCGGCCGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGCATCGCCATTAAAACCTGTACCGGGAAACAGGTGCGTCCGGACGGTCGCTACATCAATAAATCTATCGCTTA‑ACCAGCAGGCGTTGTGCCTGCGACTCCACCGCGCTCAGCGCTGGTAGTTGCTATCCACGATcagcg > 2:808374/1‑247 (MQ=255) ttCCAGGTGAACTTGTCTTCCCGGAATCCATTGTCCGC‑ACCGTGGTGCATATGATGGAAAGCGTGGCGCTACCGGGCGGCGGCGGCGTGCAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCGGGACCGCGAAAAAGGTCGGGCCGGACGGTCGCTACATCCATAAATATATTGCTTCTACC‑GCCGGCGTTGCGCCTGCGAGTCATCCGCGCTTCGCCCGGGTTGTTGTTATCACAGATCCGcgg > 2:275028/1‑249 (MQ=255) ggTGAACGTGTCTTCCCGGAATCCATTGTCCGC‑ACCGTGGTGCATATGATGGAAAGCGTGGCGCTACCGGGCGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCGGTACCGCGAAACAGGTCGGGCCGGACGGTCGCTACATCAATAAATATATTGCTTATACC‑GCAGGCGTTGCGCCTGCGAGTCAGCCGCGCTTCGCGCTGGTTGTTGTTATCAACGACCCGCAGGCggg > 2:332498/1‑251 (MQ=255) tGAACGTTTCTTCCCGGAATCCATTGTCCGC‑ACCGTGGTGCATATGATGGAAAGCGTGGCGCTACCGGGCGGAGGCGCCGTGACGGCGGCGATTACAGGCTATCGTATCGCCATTAAAACCGGTACCGCGAAAAAGGTCGGGCCGGACGGTCGCTACATCAATAACTATATTGCTTATACC‑GCAGGCGTTGCGCCTGCGAGTCAGCCGCGCTTCGCGCTGGTTGTTGTTATCAACGATCCGCAGGAGGGtg > 2:47763/1‑250 (MQ=255) tGAACGTGTCTTCCCGGAATCCATTGTCCGC‑ACCGTGGTGCATATGATGGAAAGCGTGGCGCTACCGGGCGGCGGAGGCGTGAAGGCGGCGATTAAAGGCTATAGTATCGCCATTAAAACCGGTACCGCGAAAAAGGTCGGGCCGGACGGTCGCTACATCAATAAATATATTGCTTATACC‑GCAGGCGTTGCGCCTGCGAGTCAGCAGCGCTTCGCGCTGGTTGTTGTTATCACCGATCCGCAGGGGGGt > 1:913356/1‑250 (MQ=255) aaCGTGTCTTCCCGGAATCCATGGTCCGC‑ACCGTGGTGCATATGATGGAAAGCGTGGCGCTACCGGGCGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCGGTACCGCGAAAAAGGTCGGGCCGGACGGTCGCTACATCAATAAATATATTGCTTATACC‑GCAGGCGTTGCGCCTGCGAGTCAGCCGCGCTTCGCGCTGGTTGTTGTTATCAACGATCCGCAGGCGGGTaaa < 1:32346/251‑1 (MQ=255) ggttttgtcccgtattcctttGTCCGC‑ACCGTGTTGTATATGATTGAAAGCGGTGCGCTACCGGGCGGCGGCGGCGTGAAGGCGGCGTTTAAAGGCTATCGTATCGCCATTAAATCCGGTACCGCGAAAAAGGTCGGGCCGGACGGTCGCTACAGCAATAAATATATTGCTTATACC‑GCAGGCGTTGCGCCTGCGAGTCAGCCGCGCTTCGCGCTGGTTGTTGTTATCAACGATCCGCAGTCGGGTAAAta < 2:863883/232‑1 (MQ=255) gtgtCTTCCCGGAATCCATTGTCCGC‑ACCGTGGTGCATATGATGGAAAGCGTGGCGCTACCGGGCGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCGGTACCGCGAAAAAGGTCGGGCCGGACGGTCGCTACATCAATAAATATATTGCTTATACC‑GCAGGCGTTGCGCCTGCGAGTCAGCCGCGCTTCGCGCTGGTTGTTGTTATCAACGATCCGCAGGCGGGTGAAta > 1:520287/1‑250 (MQ=255) gtCTTCCCGGAATCCATTGTCCGC‑ACCGTGGTGCATATGATGGAAAGCGTGGCGCTACCGGGCGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCGGTACCGCGAAAAAGGTCGGGCCGGACGGTCGCTACATCAATAAATATATTGCTTATACC‑GCAGGCGTTGCGCCTGCGAGTCAGCCGCGCTTCGCGCTGGTTGTTGTTATCAACGATCCGCAGGCggggtaaaact > 1:170257/1‑242 (MQ=255) ttCCCGGAATCCATTGTCCGC‑ACCGTGGTGCATATGATGGAAAGCGTGGCGCTACCGGGCGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCGGTACCGCGAAAAAGGTCGGGCCGGACGGTCGCTACATCAATAAATATATTGCTTATACC‑GCAGGCGTTGCGCCTGCGAGTCAGCCGCGCTTCGCGCTGGTTGTTGTTATCAACGATCCGCAGGCGGGGAAAaactaccg > 1:607032/1‑249 (MQ=255) tCCCGGAATCCATTGTCCGC‑ACCGTGGTGCATATGATGGAAAGCGTGGCGCTACCGGGCGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCGGTACCGCGAAAAAGGTCGGGCCGGACGGTCGCTACATCAATAAATATATTGCTTATACC‑GCAGGCGTTGCGCCTGCGAGTCAGCCGCGCTTCGCGCTGGTTGTTGTTATCAACGATCCGcgggcggggtaaaaacacgcg > 2:415294/1‑238 (MQ=255) ggAATCCATTGTCCGC‑ACCGTGGGGCATATGATGGAAAGCGTGGCGCTACCGGGCGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCGGTACCGCGAAAAAGGTCGGGCCGGACGGTCGCTACATCAATAAATATATTGCTTATACC‑GCAGGCGTTGCGCCTGCGAGTCAGCCGCGCTTCGCGCTGGTTGTTGTTATCAACGATCCGCAGGCGGGTAAATACTACGgcggcg < 2:607032/251‑1 (MQ=255) aatcacttggccGC‑ACCGTGGGTCATATGATGGAATGCGTGGCGCTACCGGGCGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCGGTACCGCGAAAAAGGTCGGGCCGGACGGTCGCTACATCAATAAATATATTGCTTATACC‑GCAGGCGTTGCGCCTGCGAGTCAGCCGCGCTTCGCGCTGGTTGTTGTTATCAACGATCCGCAGGCGGGTAAATACTACGGCGGTGcc < 2:687896/241‑1 (MQ=255) aaTCCATTGTCCGC‑ACCGTGGGGCAGATGATGGAAAGCTTGGCGCTACCGGGGGGCTGCGGCTTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCGGTACCGCGAAAAATGTCGGGCCGGACGGTCGCTACATCAATAAATATATTGCTTATACC‑GCAGGCGTTGCGCCTGCGAGTCAGCCGCGCTTCGCGCTGGTTGTTGTTATCAACGATCCGCAGGCGGGTAAATACTACGGCGGCGcc < 2:588883/251‑1 (MQ=255) ccatggtccgcaccggggggcaatggatgGAAAGGGTGGCGCTACCGGTGGGCTGCGGAGTGAAGTCGGCGATGAAAGGCTATCGTATCGCCATTAAAACCGGTACCGCGAAAAAGGTCGGGCCGGACGGGCGCTACATCAATAAATATATTGCTTATACC‑GCAGGCGTTGCGCCTGCTAGTCAGCCGCGCTTCGCGCTGGTTGTTGTTATCAACGATCCGCAGGCGGGTAAATACTACGGCGGCGCC‑G‑t < 2:363615/225‑1 (MQ=255) catggTCCGT‑ACCGTGGGGCATATGATGGAAAGCGTGGCGTTACCGGGCGGCGGAGGTGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCGGTACCGCGAAAAAGGTCGGGCCGGACGGTCGCTACATCAATAAATATATTGCTTATACC‑GCAGGCGTTGCGCCTGCGAGTCAGCCGCGCTTCGCGCTGGTTGTTGTTATCAACGATCCGCAGGCGGGTAAATACTACGGCGGCGCC‑G‑ttt < 2:442570/247‑1 (MQ=255) cATTGTCCGC‑ACCGTGGTGCATATGATGGAAAGCGTGGCGCTACCGGGCGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCGGTACCGCGAAAAAGGTCGGGCCGGACGGTCGCTACATCAATAAATATATTGCTTATACC‑GCAGGCGTTGCGCCTGCGAGTCAGCCGCGCTTCGCGCTGGTTGTTGTTATCAACGATCCGCAGGCGGGGAAATACTTcgggggggcccttt > 1:361998/1‑240 (MQ=255) agtGTCCGC‑CCCGTGGGGCATAGGATGGAAAGCGTGGCGCTCCCGGGCGGCGGCGGCGTGAAGGCGGCGATTAAAGGCGATCGTAGCGCCATTAAAACCGGTACCGCGAAAAAGGTCGGGCCGGACGGTCGCTACATCAATAAATATATTGCTTATACC‑GCAGGCGTTGCGCCTGCGAGTCAGCCGCGCTTCGCGCTGGTTGTTGTTATCAACGATCCGCAGGCGGGTAAATACTACGGCGGCGCC‑G‑TTTc < 2:513631/249‑1 (MQ=255) aTTGTCCGC‑ACCGTGGTGCATATGATGGAAAGCGTGGCGCTACCGGGCGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCGGTACCGCGAAAAAGGTCGGGCCGGACGGTCGCTACATCAATAAATATATTGCTTATACC‑GCAGGCGTTGCGCCTGCGAGTCAGCCGCGCTTCGCGCTGGTTGTTGTTATCAACGATCCGCAGGCGGGTAAATACTACGGCGGGGCC‑C‑tttt > 1:338377/1‑250 (MQ=255) gC‑ACCGTGGTGCATATGATGGAAAGCGTGGCGCTACCGGGCGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCGGTACCGCGAAAAAGGTCGGGCCGGACGGTCGCTACATCAATAAATATATTGCTTATACC‑GCAGGCGTTGCGCCTGCGAGTCAGCCGCGCTTCGCGCTGGTTGTTGTTATCAACGATCCGCAGGCGGGTAAATACTACGGCGGCGCC‑G‑TTTCCG‑CGCCg < 1:421512/251‑1 (MQ=255) cgccccgtggtgCATATGATGGAAAGCGTGGCGCTACCGGGCGGCGGCGGCTTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCGGTACCGCGAAAAAGGGCGGGCCGGACGGTCGCTACATCAATAAATATATTGCTTATGCC‑GCAGGCGTTGCGCCTGCGAGTCAGCCGCGCTTCGCGCTGGTTGTTGTTATCAACGATCCGCAGGCGGGTAAATACTACGGCGGCGCC‑G‑TTTCCG‑CGcc < 2:906758/245‑1 (MQ=255) ccggtggggaaaatgatgTAAAGCGGGGGGCTACCGGGAGGCGGCGGCGTGAAGGCGGTGATGAAAGGCTATCTGATCGCCATTAAAACCGGTACCGCGAAAAGGGGCGGGCCGGACGGTCGCTACATCAATAAATATATTGCTGATACC‑GCAGGCGTTGCGCCTGCGAGTCAGCCGCGCTTCGCGCTGGTTGTTGTTATCAACGATCCGCAGGCGGGTAAATTCTACGGCGGCGCT‑G‑TTTCCG‑CGCCGGt < 2:390056/239‑1 (MQ=255) accgtggtgCATATGATGGAAAGCGTGGCGCTACCGGGCGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCGGTACCGCGAAAAAGGTCGGGCCGGACGGTCGCTACATCAATAAATATATTGCTTATACC‑GCAGGCGTTGCGCCTGCGAGTCAGCCGCGCTTCGCGCTGGTTGTTGTTATCAACGATCCGCAGGCGGGTAAATACTACGGCGGCGCC‑G‑TTTCCG‑CGCCGGt < 1:551043/248‑1 (MQ=255) accgtggtgCATATGATGGAAAGCGTGGCGCTACCGGGCGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCGGTACCGCGAAAAAGGTCGGGCCGGACGGTCGCTACATCAATAAATATATTGCTTATACC‑GCAGGCGTTGCGCCTGCGAGTCAGCCGCGCTTCGCGCTGGTTGTTGTTATCAACGATCCGCAGGCGGGGAAAAACTACGGCGGGGCCCG‑TTTCC‑‑CGCCggg > 1:875211/4‑250 (MQ=255) tggtgCATATGATGGAAAGCGTGGCGCTACCGGGCGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCGGTACCGCGAAAAAGGTCGGGCCGGACGGTCGCTACATCAATAAATATATTGCTTATACC‑GCAGGCGTTGCGCCTGCGAGTCAGCCGCGCTTCGCGCTGGTTGTTGTTATCAACGATCCGCAGGCGGGGAAATTATACGGCCGCGCA‑G‑GTTCCGGCGCCggcatt > 1:109448/1‑247 (MQ=255) gtgCATATGATGGAAAGCGTGGCGCTACCGGGCGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCGGTACCGCGAAAAAGGTCGGGCCGGACGGTCGCTACATCAATAAATATATTGCTTATACC‑GCAGGCGTTGCGCCTGCGAGTCAGCCGCGCTTCGCGCTGGTTGTTGTTATCAACGATCCGCAGGCGGGGGAATACTAcggcgggggcgctttcgcggcggggttttt > 1:109578/1‑227 (MQ=255) gtgCATATGATGGAAAGCGGGGCGCGACCGGGCGGCGGGGGCGTGAAGGCGGCGATTAAAGGCTAGCGTATCGCCATTAAAACCGGTACCGCGAAAAAGGTCGGGCCGGACGGTCGCTACATCAATAAATATATTGCTTATACC‑GCAGGCGTTGCGCCTGCGAGTCAGCCGCGCTTCGCGCTGGTTGTTGTTATCAACGATCCGCAGGCGGGTAAATACTACGGCGGCGCC‑G‑TTTCCG‑CGCCGGTCTTTgg < 2:208145/251‑1 (MQ=255) cATAGGATTGAAAGCGGGGCGCTACCGGGCGGCGCCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCGGTACCTCGAAAAAGGTCGGGCCGGACGGTCGCTACATCAATAAATATATTGCTTATACC‑GCAGGCGTTGCGCCTGCGAGGCAGCCGCGCTTCGCGCTGGTTGTTGTTATCAACGATCCGCAGGCGGGTaaa < 2:262849/213‑1 (MQ=255) gAAAGCGTGGCGTTACCGGGCGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCGGTACCGCGAAAAAGGTCGGGCCGGACGGTCGCTACATCAATAAATATATTGCTTATACC‑GCAGGCGTTGCGCCTGCGAGTCAGCCGCGCTTCGCGCTGGTTGTTGTTATCAACGATCCGCAGGCGGGTAAATACTACGTCGGCGCC‑G‑TTTCTG‑CGCCGGTCTTTGGTGCCATCATggg < 1:853593/251‑1 (MQ=255) gAAAGCGTGGCGCTACCGGGCGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCGGTACCGCGAAAAAGGTCGGGCCGGACGGTCGCTACATCAATAAATATATTGCTTATACC‑GCAGGCGTTGCGCCTGCGAGTCAGCCGCGCTTCGCGCTGGTTGTTGTTATCAACGATCCGCAGGCGGGGAAATACTACGGCGGGGCC‑GGTTTCC‑‑CGCCGGGCTTTGGTggcctcacggg > 1:523161/1‑241 (MQ=255) | AGAACGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAACACCATTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCGGTGAACGTGTCTTCCCGGAATCCATTGTCCGC‑ACTGTGGTGCATATGATGGAAAGCGTGGCGCTACCAGGCGGCGGCGGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCGGTACCGCGAAAAAGGTCGGGCCGGACGGTCGCTACATCAATAAATATATTGCTTATACC‑GCAGGCGTTGCGCCTGCGAGTCAGCCGCGCTTCGCGCTGGTTGTTGTTATCAACGATCCGCAGGCGGGTAAATACTACGGCGGCGCC‑G‑TTTCCG‑CGCCGGTCTTTGGTGCCATCATGGG > NC_000913/92597‑93076 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |