Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	65,381	T→C	T134A (ACT→GCT)	polB ←	DNA polymerase II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	65,381	0	T	C	98.9%	272.0 / ‑4.4	89	T134A (ACT→GCT)	polB	DNA polymerase II
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base C (25/62);  total (27/62)
Fisher's exact test for biased strand distribution p-value = 2.95e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.34e-01

GTTGGCAAACCAGGCGTTGAGTTTTTCCAGCAACTGCGGGCGGCTGGCGACGTATTCCAGTTCGAAATCAAGCGAGGAGGCGTCGCCATTCTCCGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAATATCTATAGAAACCCACTTGAGCGGCGGA‑CGATAGT‑CGGGATGCGGTTTCAGACGGGCATTAACGATAGTGCCATTGTGCA‑TATCACCCTCGACCCACACCGGTGAGGTGAT‑AAACCGCTCCATCAGATAGCGTTCTGGCGGACGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAAA‑GCCTTGTTCACCCTGCAAAATATGCTGAGCG  >  NC_000913/65139‑65625                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                        gTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGACTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAATATCAATAGAAACCCCCTTGAGCGGCGGG‑CGATAGA‑CGGGGTGCGGGTTCAGACGGGCATTAACGATAGCGCCATtgt                                                                                                                                                                                                                                                 >  1:265431/1‑251 (MQ=255)   tGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAATATCAATAGAAACCCACTTGAGCGGCGGA‑CGATAGT‑CGGGATGCGGTTTCAGACGGGCATTAACGATAGCGCCATtgtg                                                                                                                                                                                                                                                <  1:515522/250‑1 (MQ=255)    ggCAAACCAGGCGTTGAGTTTTGCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAATATCAATAGAAACCCACTTGAGCGGCGGA‑CGATAGT‑CGGGATGCGGTTTCAGACGGGCATTAACGATAGCGCCATTGTGCa                                                                                                                                                                                                                                              <  1:581515/251‑1 (MQ=255)        aaCCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAATATCAATAGAAACCCACTTGAGCGGCGGA‑CGATAGT‑CGGGATGCGGTTTCAGACGGGCATTAACGATAGCGCCATTGTGCA‑TATc                                                                                                                                                                                                                                         <  1:153279/251‑1 (MQ=255)        aaCCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAATATCAATAGAAACCCACTTGAGCGGCGGA‑CGATAGT‑CGGGATGCGGTTTCAGACGGGCATTAACGATAGCGCCATTGTGCA‑TATc                                                                                                                                                                                                                                         <  1:48046/251‑1 (MQ=255)         accaggatttGATTTTTTCCAGTAGCTGCGGGTGGCTGGCGACGTATTCCCGTTCAAAATCGAGCGCGTCGGCCTCGCCATTCTCTGGTCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCTCCGTGGCGGGTGGTTTCAATATCAATAGAAACCCCCTTGAGCGGCGGA‑CGATAGT‑CGGGTTGCGGTTTCAGACGGGCATTAACGATAGCGCCATTGTGCA‑TATCa                                                                                                                                                                                                                                        <  1:720123/243‑1 (MQ=255)                gTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAATATCAATAGAAACCCACTTGAGCGGCGGA‑CGATAGT‑CGGGATGCGGTTTCAGACGGGCATTAACGATAGCGCCATTGTGCA‑TATCACCCTCGa                                                                                                                                                                                                                                 <  1:227137/251‑1 (MQ=255)                  tGAGTTTTTCCAGTAGTGGCCGGCGGTTGGCGACGTATGCCAGTTAAAAATCGAGCGCGGAGGCGTCGCAATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTGCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAATAGCAATAGAAACCCACTTGAGCGGCTGA‑CGATAGT‑CGGGATGCGGTTTCAGACGGTCATTAACGATAGCGCCATTGTGCA‑TATCACCCTCGAc                                                                                                                                                                                                                                <  2:327960/250‑1 (MQ=255)                   gaatttttCCAGGAGCGGCGGGCGGGTGGCGACGTATTCCAGTGCAAAACGTAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAATATCAATAGAAACCCACTTGAGCGGCGGA‑CGATAGT‑CGGGATGCGGTTTCAGACGGGCATTAACGATAGCGCTATTGTGCA‑TATCACCCTCGAccc                                                                                                                                                                                                                              <  2:120250/248‑1 (MQ=255)                   gAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAATATCAATAAAAACCCCATTGAGCGGCGGG‑CGATAGT‑CGGGGTGCGGTTTCAGACGGGCATTAACGATAGCGCCATTGTGCA‑TATCACCCTCGAccc                                                                                                                                                                                                                              >  1:983954/1‑251 (MQ=255)                    aGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCTTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAATATCAATAGAAACCCACTTGAGCGGCGGA‑CGATAGT‑CGGGATGCGGTTTCAGACGGGCATTAACGATAGCGCCATTGTGCA‑TATCACCCTCGACCca                                                                                                                                                                                                                             <  1:747526/251‑1 (MQ=255)                          tccagtagcTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAATATCAATAGAAACCCACTTGAGCGGCGGA‑CGATAGT‑CGGGATGCGGTTTCAGACGGGCATTAACGATAGCGCCATTGTGCA‑TATCACCCTCGACCCACACCg                                                                                                                                                                                                                        <  1:324011/242‑1 (MQ=255)                            cagtagcTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAATATCAATAGAAACCCACTTGAGCGGCGGA‑CGATAGT‑CGGGATGCGGTTTCAGACGGGCATTAACGATAGCGCCATTGTGCA‑TATCACCCTCGACCCACACCggtg                                                                                                                                                                                                                     <  1:919965/245‑1 (MQ=255)                                agcTGCGGTCGGCTGGCGACGTATTCCATTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAATATCAATAGAAACCCACTTGAGCGGCGGA‑CGATAGT‑CGGGATGCGGTTTCAGACGGGCATTAACGATAGCGCCATTGTGCA‑TATCACCCTCGACCCACACCggtgagg                                                                                                                                                                                                                  <  2:246798/248‑1 (MQ=255)                                 gcTGCGGGCGGCTGGCGACGTATTCAAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGGGGGTTCCATATCAAAAGAAACCCCCTTTAGCGGCGGG‑CGATAGTTGGGGAGGGGGTTT‑AGACCGGCGTTAACACTAGCGCCATGTTGCA‑TATCACCCCCCACCCACCCCggggggggg                                                                                                                                                                                                                >  2:65218/2‑244 (MQ=255)                                      gggcggCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAGCGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAATATCAATAGAAACCCACTTGAGCGGCGGA‑CGATAGT‑CGGGATGCGGTTTCAGACGGGCATTAACGATAGCGCCATTGTGCA‑TATCACCCTCGACCCACACCGGTGAGGTGAT‑aaa                                                                                                                                                                                                          <  1:727347/251‑1 (MQ=255)                                      gggcggCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCCATATCAATAGAAACCCACTTGAGCGGCGGA‑CGATAGT‑CGGGATGCGGGTTCAGACGGGCATTAACGCAAGAGCCCTTGTGCG‑TATCACCCCCGACCCACACCggtgaggggctaa                                                                                                                                                                                                            >  1:91370/1‑244 (MQ=255)                                      gggcggCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAATATCAATAGAAACCCACTTGAGCGGCGGA‑CGATAGT‑CGGGATGCGGTTTCAGACGGGCATTAACGATAGCGCCATTGTGCA‑TATCACCCTCGACCCACACCGGTGAGGTGAT‑aaa                                                                                                                                                                                                          <  1:672799/251‑1 (MQ=255)                                          ggcTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGGGGTTTCAATATCAATAGAAACCCACTTGAGCGGCGGA‑CGATAGT‑CGGGATGCGGTTTCAGACGGGCATTAACGATAGCGCCATTGTGCA‑TATCACCCTCGACCCACCCCGGTGGGGTGAT‑AAACCGc                                                                                                                                                                                                      >  1:258590/1‑251 (MQ=255)                                           gcTGGCGACGTATTCCAGTTCAAAATCGATCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGATTCAATATCAAAAGAAACCCACTTGAGGGGCGGAAGGATA‑T‑CCGGGTGGGGTTTTAGACGGGGATTAACGAAAGCGCCATTTTGAAACACCACC‑TCGATCCCCACCGGGGAGGGGAT‑AAACCGcc                                                                                                                                                                                                     >  1:162664/1‑250 (MQ=255)                                              ggCGACGTATTCCAGTTCAAAAGCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCGCACCGTGGCGGGTGGTTTCAATAGCAATAGAAACCCACTTGAGCGGCGGA‑CGATAGT‑CGGGATGCGGTTTCAGACGGGCATTAACGATAGCGCCATTGTGCA‑TATCACCCTCGACCCACACCGGTGAGGTGAT‑AAACCGCTc                                                                                                                                                                                                    <  2:293319/249‑1 (MQ=255)                                               gCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAATATCAATAGAAACCCACTTGAGCGGCGGA‑CGATAGT‑CGGGATGCGGTTTCAGACGGGCATTAACGATAGCGCCATTGTGCA‑TATCAACCTCGACCCACACCGGTGAGGTGAT‑AAACCGCTCCAt                                                                                                                                                                                                 <  1:229483/251‑1 (MQ=255)                                                      aTTCCAGTTCAAAACCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAATATCAATAGAAACCCACTTGAGCGGCGGA‑CGATAGT‑CGGGATGCGGTTTCAGACGGGCATTAACGATAGCGCCATTGTGCA‑TATCACCCTCGACCCACACCGGTGAGGTGAT‑AAACCGCTCCATCAGATAg                                                                                                                                                                                          <  2:268797/251‑1 (MQ=255)                                                         ccAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAATATCAATAGAAACCCACTTGAGCGGCGGA‑CGATAGT‑CGGGATGCGGTTTCAGACGGGCATTAACGATAGCGCCATTGTGCA‑TATCACCCTCGACCCACACCGGTGAGGTGAT‑AAACCGCTCCATCAGATAGCg                                                                                                                                                                                        <  1:128705/250‑1 (MQ=255)                                                         ccAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAATATCAATAGAAACCCACTTGAGCGGCGGA‑CGATAGT‑CGGGATGCGGTTTCAGACGGGCATTAACGATAGCGCCATTGTGCA‑TATCACCCTCGACCCACACCGGTGAGGTGAT‑AAACCGCTCCATCAGATAGCGt                                                                                                                                                                                       <  1:194925/251‑1 (MQ=255)                                                                     cgaGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAATATCAATAGAAACCCACTTGAGCGGCGGA‑CGATAGT‑CGGGATGCGGTTTCAGACGGGCATTAACGATAGCGCCATTGTGCA‑TATCACCCTCGACCCACACCGGTGAGGTGAT‑AAACCGCTCCATCAGATAGCGTTCTGGCgggcg                                                                                                                                                                            <  1:775717/248‑4 (MQ=255)                                                                       aGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAATATCAATAGAAACCCACCTGCGCGGCGGA‑CGGTAGT‑CGGGGTGCGGTTTCAGACGGGCATTAACGCTAGCGCCATTATGCA‑TATCACCCTCGACCCACCCCGGCGGGGGGATTAAACC‑CTCCACCCGACTGCGTTCTGGCgggcccac                                                                                                                                                                         >  1:481367/1‑245 (MQ=255)                                                                              aggCGTCGCTATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCTCCGTGGCGGGTGGTTTCAATATCAATAGAAACCCTCTTGAGCGGCGGA‑CGATAGT‑CGGGATGCGGTTTCAGACGGGCATTAACGATAGCGCCATTGTGCA‑TATCACCCTCGACCCACACCGGTGAGGTGAT‑AAACCGCTCCATCAGATAGCGTTCTGGCGGGCGCACATCGGcc                                                                                                                                                                  <  1:545924/251‑1 (MQ=255)                                                                                             ctggCCCCAGCATATAAACGATGCGCGGCCCGCATCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCTATATCAATAGAAACCCACTTGAGCGGCGGA‑CGATAGT‑CGGGATGCGGTTTCAGACGGGCATTAACGATAGCGCCATTGTGCA‑TATCACCCTCGACCCACACCGGTGAGGTGAT‑AAACCGCTCCATCAGATAGCGTTCTGGCGGGCGCACATCGGCCTCGTAGACGGTa                                                                                                                                                      <  2:741151/246‑1 (MQ=255)                                                                                                 ccccAGCAAATAAACGATGCGCTGGCCGCATCCTTCCAGGACGATGCAGTACAGCTCCCCGTGGCGGGTGGTTTCAATATCAATAGAACCCCTCTTGAGCGGCGGA‑CGATAGT‑CGGGATGCGGTTTCAGACTGGCCTTAACGATAGCGCCAGTGTGCA‑TATCACCCTCGACCCACACCGGTGAGGTGAT‑AAACCGCTTCATCAGATAGCGTTCTGGCGGGCGCACATCGGCATCGTAGACGGTAACGCCAc                                                                                                                                               <  2:599190/251‑1 (MQ=255)                                                                                                   ccAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAATATCAATAGAAACCCACTTGAGCGGCGGA‑CGATAGT‑CGGGGTGCGGTTTCAGACGGGCATTAACGATAGCGCCATTGTGCA‑TATCACCCTCGACCCACACCGGTGAGGGGAT‑AAACCGCTCCATCCGATAGCGTTCTGGCGGGGGCCCATCGGCCTCGTAGGCgggaaacccaccc                                                                                                                                             >  1:671514/1‑240 (MQ=255)                                                                                                    cAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAATATCAATAGAAACCCACTTGAGCGGCGGA‑CGATAGT‑CGGGATGCGGTTTCAGACGGGCATTAACGATAGCGCCATTGTGCA‑TATCACCCTCGACCCACACCGGTGAGGTGAT‑AAACCGCTCCATCAGATAGCGTTCTGGCGGGCGCACATCGGCCTCGTAGACGGTAACGCCACCtt                                                                                                                                            <  1:805450/251‑1 (MQ=255)                                                                                                      gcttataAACGAGTCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCATCGTGGCGGGTGGTTTCAATATCAATAGAAACCCCCTTGAGCGGCGGA‑CGATAGT‑CGGGATGCGGTTTCAGACGGGCATTAACGATAGCGCCATTGTGCA‑TATCCACCTCGACCCACACCGGTGAGGTGAT‑AAACCGCTCCATCAGATAGCGTTCTGGCGGGCGCACATCGGCCTCGTAGACGGTAACGCCACCTTCa                                                                                                                                          <  2:936929/248‑1 (MQ=255)                                                                                                         tataaacgtcggccTGCCCGCAGCCTTCCAGGCCGATGTAGTACAGCTCCCCGTGGCGGGTGGTTTCAAGATCAATAGAAACCCACTGGAGCGGCGGA‑CGATAGT‑CGGGATGCGGTTTCAGACGGGCATTAACGATAGCGCCATTGTGCA‑TATCACCCTCGACCCACACCGGTGAGGTGAT‑AAACCGCTCCATCAGATAGCGTTCTGGCGGGCGCACATCGGCCTCGTAGACGGTAACGCCACCTTCAc                                                                                                                                         <  2:635403/236‑1 (MQ=255)                                                                                                                  tGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGGTTCAATTTCAATAGAAACCCACTTGAGCGGCGGA‑CGATAGT‑CGGGATGCGGGTTCAGACGGGCATTAACGATAGCGCCATTGTGAA‑TATCACCCTCGACCCACACCGGTGAGGTGGT‑AAACCACCCCCTCCGATAGCGTTCTGGCCGGCGCACATCGGCCTCGGAGACCGTAACGCCACCCTCACCCAGGCGCttt                                                                                                                              >  1:728008/1‑251 (MQ=255)                                                                                                                   gcgcTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAATATCAATAGAAACCCACTTGAGCGGCGGA‑CGATAGT‑CGGGATGCGGTTTCAGACGGGCATTAACGATAGCGCCATTGTGCA‑TATCACCCTCGACCCACACCGGTGAGGTGAT‑AAACCGCTCCATCAGATAGCGTTCTGGCGGGCGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCttt                                                                                                                              <  1:67137/250‑1 (MQ=255)                                                                                                                    cgcTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAATATCAATAGAAACCCACTTGAGCGGCGGA‑CGATAGT‑CGGGATGCGGTTTCAGACGGGCATTAACGATAGCGCCATTGTGCA‑TATCACCCTCGACCCACACCGGTGAGGTGAT‑AAACCGCTCCATCAGATAGCGTTCTGGCGGGCGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTc                                                                                                                            <  1:81235/251‑1 (MQ=255)                                                                                                                    cgcTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAATATCAATAGAAACCCACTTGAGCGGCGGA‑CGATAGT‑CGGGATGCGGTTTCAGACGGGCATTAACGATAGCGCCATTGTGCA‑TATCACCCTCGACCCACACCGGTGAGGTGAT‑AAACCGCTCCATCAGATAGCGGTCTGGCGGGCGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTc                                                                                                                            <  1:410925/251‑1 (MQ=255)                                                                                                                    cgcTGCCCGCAGCCTGCCAGGCCGATGCAGTACAGCGCACCGTGGCGGGTGGTTTCAATATCAATAGAAACCCACTTGAGCGGCGGA‑CGATAGT‑CGGGATGCGGTTTCAGACGGGCATTAACGATAGCGCCATGGTGCA‑TATCACCCTCGACCCACACCGGTGAGGTGAT‑AAACCGCTCCATCAGATAGCGTTCTGGCGGGCGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTc                                                                                                                            <  2:290196/251‑1 (MQ=255)                                                                                                                             cAGCCTTCCATGACGATGCAGTACAGCTCACCGTTGCGGGTGGTTTCAATAGCAATAGAAACCCACTTTAGCGGCGGA‑CGATAGT‑CGGGATGCGGTTTCAGACGGGCATTAACGATAGCGCCATTGTGCA‑TATCACCCTCGACCCACACCGGGTAGGTGAT‑AAACCGCTCCATCAGATAGCGTTCTGGCGGGCGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTGCGTAATtcat                                                                                                                   <  1:940195/251‑1 (MQ=255)                                                                                                                                   tCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAATATCAATAGAAACCCACTTGAGCGGCGGA‑CGATAGT‑CGGGATGCGGTTTCAGACGGGCATTAACGATAGCGCCATTGTGCA‑TATCACCCTCGACCCACACCGGTGAGGTGAT‑AAACCGCTCCATCAGATAGCGTTCTGGCGGGCGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAAtt                                                                                                              <  1:229987/250‑1 (MQ=255)                                                                                                                                   accAGGCCGATGCCGTACAGCTCACCGTGGCGGGTGGTTTCAATATCAATAGAAACCCATTTGAGCGGCGGA‑CGATAGT‑CGGGATGCGGTTTCAGACGGGCATTAACGATAGCGCCATTGTGCA‑TATCCCCCTCGACCCACACCGGTGAGGTGAT‑AAACCGCTCCATCAGATAGCGTTCTGGCGGGCGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTGTCGTAATTCATCAAtt                                                                                                              <  2:527515/249‑1 (MQ=255)                                                                                                                                    ccAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAATATCAATAGAAACCCACTTGAGCGGCGGA‑CGATAGT‑CGGGATGCGGTTTCAGCCGGGCATTAACGATAGCGCCAGTGTGCA‑TATCACCCTCGACCCACACCGGTGAGGTGAG‑AAACCGCCCCATCAGATAGCGTTCTGGCGGGCGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTATTCGTAATTCATCaaatg                                                                                                             <  2:611592/250‑4 (MQ=255)                                                                                                                                    ccAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAATATCAATAGAAACCCACTTGAGCGGCGGA‑CGATAGT‑CGGGATGCGGTTTCAGACGGGCATTAACGATAGCGCCATTGTGCA‑TATCACCCTCGACCCACACCGGTGAGGTGAT‑AAACCGCTCCATCAGATAGCGTTCTGGCGGGCGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTg                                                                                                             <  1:833517/250‑1 (MQ=255)                                                                                                                                       ggCCGATGCAGTACAGCTCACCGGGGCGGGTGGGTTCAATATCAATAGTAACCCACTTGAGCGGCGGA‑TGATAGT‑CGGTATGCGGTTTCAGACGGGCATTAACGAGATCGACATTGTGCA‑TATCACCCGCGCCCCACACCGGTGAGGTGAT‑AAACCGCTCCATCAGATAGCGTTCTGGCGGGCGCACATCGGCCTCGTAGACTGTAACGCCACCTTCACGCAGGCGCTTGTCGTTATTCATCAAATGGCGa                                                                                                         <  2:223311/251‑1 (MQ=255)                                                                                                                                              gCAGTACAGCTCACCGTGGCGGGTGGTTTCAATATCAATAGAAACCCACTTGAGCGGCGGA‑CGATAGT‑CGGGATGCGGTTTCAGACGGGCATTAACGATAGCGCCATTGTGCA‑TATCACCCTCGACCCACACCGGTGAGGTGAT‑AAACCGCTCCATCAGATAGCGTTCTGGCGGGCGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGgcg                                                                                                   <  1:820941/250‑1 (MQ=255)                                                                                                                                                 gTACAGCTCACCGTGGCGGGTGGTTTCAATATCAATAGAAACCCACTTGAGCGGCGGA‑CGATAGT‑CGGGATGCGGTTTCAGACGGGCATTAACGATAGCGCCATTGTGCA‑TATCACCCTCGACCCACACCGGTGAGGTGAT‑AAACCGCTCCATCAGATAGCGTTCTGGCGGGCGCACATCGGCCTCGTAGACGGTAACGCCGCCTTCACGCAGGCGCTTTTCGTAATTCATCAATGGGCGATGGGCGCGAc                                                                                               <  1:282778/251‑1 (MQ=255)                                                                                                                                                     cgctcaccctggctggtttgttCAATATCAATATAAACCTTCTTGAGCGGCGGA‑CGATAGT‑CGGGATGCGGTGTCATACGGGCATTAACGATAGCGCCATTGTGCA‑CAGCACCCTCGACCCACCCCGGTGAGTTGAT‑AAACCGCTCCATCAGATAGCGTTCGGGCGGGCGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCTCGACAGTa                                                                                           <  2:545960/231‑1 (MQ=255)                                                                                                                                                                    gtgggTTCAATATCAATAGAAACCCAGTTGAGCGGCGGA‑CGATAGT‑CGGGATGCGGTTTCAGACGGGCATTAACTATAGCGCCATTGTGCA‑TATCACCCTCGACCCACACCGGTGAGGTGAT‑AATCCGCTCCATCAGATAGCGTTCTGGCGGGCGCACATCGGCCTCGTAGACGGTAACGCCACCTGCACGCAGGCGCTTTTCGTAATGCAGCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCgg                                                                            <  2:79113/251‑1 (MQ=255)                                                                                                                                                                         ttCAATATCAATAGAAACCCACTTGAGCGGCGGA‑CGATAGT‑CGGGATGCGGTTTCAGACGGGCATTAACGATAGCGCCATTGTGCA‑TATCACCCTCGACCCACACCGGTGAGGTGAT‑AAACCGCTCCATCAGATAGCGTTCTGGCGGGCGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCCATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGc                                                                       >  1:325309/1‑251 (MQ=255)                                                                                                                                                                          tcaatatcaataGAAACCCACTTGAGCGGCGGA‑CGATAGT‑CGGGATGCGGTTTCAGACGGGCATTAACGATAGCGCCATTGTGCA‑TATCACCCTCGACCCACACCGGTGAGGTGAT‑AAACCGCTCCATCAGATAGCGTTCTGGCGGGCGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACCGTAAAGGCCATACACCGGCTGGCg                                                                      >  1:758858/1‑251 (MQ=255)                                                                                                                                                                          tcaataacactaGATACCCACTTGTGCGGCGGA‑CGATAGT‑CTGGATGCGGTTTCAGACCGGCATTAACGTTAGCGCCATTGTGCA‑TATCCCCCTCGACCCACACCGGTGAGGTGAT‑AAACCGCTCCATCAGATAGCGTTCTGGCGGGCGCTCATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCTGCTGGc                                                                       <  2:731196/240‑1 (MQ=255)                                                                                                                                                                           cAATATCAATAGAAACCCACTTGAGCGGCGGA‑CGATAGT‑CGGGATGCGGTTTCAGACGGGCATTAACGATAGCGCCATTGTGCA‑TATCACCCTCGACCCACACCGGTGAGGTGAT‑AAACCGCTCCATCAGATAGCGTTCTGGCGGGCGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCgg                                                                     <  1:523670/251‑1 (MQ=255)                                                                                                                                                                             atatCAATAGAAACCCGCATTAGCGGCGGA‑CGATAGT‑CGGGATGCGGGTTCAGACGGGCATTAACGATAGCGCCATTTTGCC‑TATCACCCTCGACCCAATCCGGTGAGGTGAT‑AAACCTCTCCAGCAGATAGCGTTCTGGCGGGCGCACATCGGCCTCGTAGACGGTAACGGCTCCTTCACGCAGGCGCTTTTCGTAATTCATCAAGTGGCGATGGGCGCGACAGTAAAGGCCATACCACGGCTGGCGGTg                                                                   <  2:325352/251‑1 (MQ=255)                                                                                                                                                                               atcaataGAAACCCACTTGAGCTGCGGA‑CGATATT‑CTGGATGCGGTTTCAGACGGGCCTTAACGATAGCGCCATTGTGCA‑TATCACCCTCGACCCACACCTGTGAGGTGAT‑AAACCGCTCCATCAGATAGCGTTCTGGCGGGCGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTGa                                                                  <  1:440794/246‑1 (MQ=255)                                                                                                                                                                               atcaataGAAACCCACTTGAGCGGCGGA‑CGATAGT‑CGGGATGCGGTTTCAGACGGGCATTAACGATAGCGCCATTGTGCA‑TATCACCCTCGACCCACACCGGTGAGGTGAT‑AAACCGCTCCATCAGATAGCGTTCTGGCGGGCGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTGa                                                                  <  1:820948/246‑1 (MQ=255)                                                                                                                                                                                 caataGAAACCCACTTGAGCGGCGGA‑CGATAGT‑CGGGATGCGGTTTCAGACGGGCATTAACGATAGCGCCATTGTGCA‑TATCACCCTCGACCCACACCGGTGAGGTGAT‑AAACCGCTCCATCAGATAGCGTTCTGGCGGGCGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGGAAAGGCCATACACCGGCTGGCGGTGaaa                                                                >  1:959553/3‑250 (MQ=255)                                                                                                                                                                                     aGAAACCCACTTGAGCGGCGGA‑CGATAGT‑CGGGATGCGGTTTCAGACGGGCATTAACGATAGCGCCATTGTGCA‑TATCACCCTCGACCCACACCGGTGAGGTGAT‑AAACCGCTCCATCAGATAGCGTTCTGGCGGGCGCACATCGGCCTCGGAGACGGAAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCAACAATTGGCGAAGGGCGCGACCGTAAAGGACATACACCGGCAGGCGGTGAAAATccc                                                           >  1:586202/1‑250 (MQ=255)                                                                                                                                                                                      gAAACCCACTTGAGCGGCGGA‑CGATAGT‑CGGGATGCGGTTTCAGACGGGCATTAACGATAGCGCCATTGTGCA‑TATCACCCTCGACCCACACCGGTGAGGTGAT‑AAACCGCTCCATCAGATAGCGTTCTGGCGGGCGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTGAAAATCCtt                                                          <  2:522318/251‑1 (MQ=255)                                                                                                                                                                                      gAAACCCACTTGAGCGGCGGA‑CGATAGT‑CGGGATGCGGTTTCAGACGGGCATTAACGATAGCGCCATTGTGCA‑TATCACCCTCGACCCACACCGGTGAGGTGAT‑AAACCGCTCCATCAGATAGCGTTCTGGCGGGCGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGGGGAAATCCtt                                                          >  1:827012/1‑251 (MQ=255)                                                                                                                                                                                          cccACTTGAGCGGCGGA‑CGATAGT‑CGGGATGCGGTTTCAGACGGGCATTAACGATAGCGCCAGTGTGCA‑TATCACCCCCGACCCACACCGGTGAGGGGAT‑AAACCGCTCCATCAGATAGCGTTCTGGCGGGCGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTGAAAATCCTTTAAc                                                      <  2:118419/251‑1 (MQ=255)                                                                                                                                                                                                 gAGCGGCGGA‑CGATAGT‑CGGGATGCGGTTTCAGACGGGCATTAACGATAGCGCCATTGTGCA‑TATCACCCTCGACCCACACCGGTGAGGTGAT‑AAACCGCTCCATCAGATAGCGTTCTGGCGGGCGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATTAATTGGCGATGGGCGAGACAGGAAAGGCCATACACCGGCTGGCGGTGAAAATACTTTAAAGCCAGCg                                               >  1:360771/1‑251 (MQ=255)                                                                                                                                                                                                      gcggA‑CGATAGT‑CGGGATGCGGTTTCAGACGGGCATTAACGATAGCGCCATTGTGCA‑TATCACCCTCGACCCACACCGGTGAGGTGCT‑AAACCGCTCCATCAGATAGCGTTCTGGCGGGCGCACATCGGCCTCGTAGACGGTAACGCCCCCTTCACGCAGGCGCTTTTCGTAATTCCTCAATTGGCGATGGGCGCGACAGTAACGGCCATCCACCGGCTGGCGGGGGAAATCCttttaagccacggggggt                                          >  2:570352/1‑236 (MQ=255)                                                                                                                                                                                                        ggA‑CGATAGT‑CGGGATGCGGTTTCAGACGGGCATTAACGATAGCGCCATTGTGCA‑TATCACCCTCGACCCACACCGGTGAGGTGAT‑AAACCGCTCCATCAGATAGCGTTCTGGCGGGCGCACCTCGGGCTCGTAGAAGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCCAGTGGCGATGGGCGCAGCAGAAAAAGCCCACCACCGGCTGGCGGTGGAAATCCTTTAAAGCCACCgggggt                                          >  2:379738/1‑245 (MQ=255)                                                                                                                                                                                                        ggA‑CGATAGT‑CGGGATGCGGTTTCAGACGGGCATTAACGATAGCGCCATTGTGCA‑TATCACCCTCGACCCACACCGGTGAGGTGAT‑AAACCGCTCCATCAGATAGCGTTCTGGCGGGCGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAg                                        <  1:542203/251‑1 (MQ=255)                                                                                                                                                                                                            gagagaggcGGGATGTGGGTTCGGAGGGGCATTAACGAAAGCGCCAGTGTGCA‑GATCACCCGCGACCCGCACCGGTGAGGTGTT‑GAACCGCTCCAACAGATAGCGTTCTTGCGGGCGCGCATCGGCCTCGTAGACGGTAACGCCACCCTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCTACAGTAAAGGCGATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGc                                      <  2:330562/243‑1 (MQ=255)                                                                                                                                                                                                            cGATAGT‑CGGGATGCGGTTTCAGACGGGCATTAACGATAGCGCCATTGTGCA‑TATCACCCTCGACCCACACCGGTGAGGTGAT‑AAACCGCTCCATCAGATAGCGTTCTGGCGGGCGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCg                                     <  1:568653/251‑1 (MQ=255)                                                                                                                                                                                                             gATAGT‑CGGGATGCGGTTTCAGACGGGCATTAACGATAGCGCCATTGTGCA‑TATCACCCTCGACCCACACCGGTGAGGTGAT‑AAACCGCTCCATCAGATAGCGTTCTGGCGGGCGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGa                                    <  1:610936/251‑1 (MQ=255)                                                                                                                                                                                                             gATAGT‑CGGGATGCGGTTTCAGACGGGCATTAACGATAGCGCCATTGTGCA‑TATCACCCTCGACCCACACCGGTGAGGTGAT‑AAACCGCTCCATCAGATAGCGTTCTGGCGGGCGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGGGAAAATCCTTTAACGCCAGCGGTGTCAGGCg                                     >  1:358833/1‑250 (MQ=255)                                                                                                                                                                                                                aGT‑CGGGATGCGGTTTCAGACGGGCATTAACGATAGCGCCATTGTGCA‑TATCACCCTCGACCCACACCGGTGAGGTGAT‑AAACCGCTCCATCAGATAGCGTTCTGGCGGGCGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGaac                                  <  1:713780/250‑2 (MQ=255)                                                                                                                                                                                                                 gT‑CGGGATGCGGTTTCAGACGGGCATTAACGATAGCGCCATTGTGCA‑TATCACCCTCGACCCACACCGGTGAGGTGAT‑AAACCGCTCCATCAGATAGCGTTCTGGCGGGCGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGGGGCAGGCGaacgc                                >  1:70983/1‑248 (MQ=255)                                                                                                                                                                                                                 gT‑CGGGATGCGGTTTCAGACGGGCATTAACGATAGCGCCATTGTGCA‑TATCACCCTCGACCCACACCGGTGAGGTGAT‑AAACCGCTCCATCAGATAGCGTTCTGGCGGGCGCACATCGGCCTCGTAGAAGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGGTAAAATCCCTTAACACCACCGGAGTccgggcaaacc                                >  1:112491/1‑241 (MQ=255)                                                                                                                                                                                                                   gcGGGATGCGGTTCCAGTCGGGCATTAACGATAGCGCCATTGTCCA‑TATCCCCCTCGACCCACACCGGGGAGGTGAT‑AAACCGCTCCATCAGATAGCGTTCTGGCGGGCGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATGGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAAC‑Gcc                              <  2:838653/250‑1 (MQ=255)                                                                                                                                                                                                                    cGGGATGCGGTTGCAGACGGGCATTAACGATAGCGCCATTGTGCA‑TATCACCCTCGACCCACACCGGTGAGGTGAT‑AAACCGCTCCATCAGATAGCGTTCTGGCGGGCGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCAGCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAAC‑Gcc                              <  1:153063/250‑1 (MQ=255)                                                                                                                                                                                                                      ggATGCGGTTTCAGACGGGCATTAACGATAGCGCCATTGTGCA‑TATCACCCTCGACCCACACCGGTGAGGTGAT‑AAACCGCTCCATCAGATAGCGTTCTGGCGGGCGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTGTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAAC‑GCCTTg                           <  1:629644/251‑1 (MQ=255)                                                                                                                                                                                                                          gCGGTTTCAGTCGGGCATTAACGATAGCGCCTTTGTTCA‑TATCACCCTCGTCCCACACCGGTGAGGTGAT‑AAACCGCTCCATCAGATAGCGTTCTGGCGGGCGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGAGGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAAC‑GCCgtgtaca                       <  1:34572/251‑4 (MQ=255)                                                                                                                                                                                                                             gTTTCAGACGGGCATTAACGATAGCGCCATTGTGCA‑TATCACCCTCGACCCACACCGGTGAGGTGAT‑AAACCGCTCCATCAGATAGCGTTCTGGCGGGCGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATAACCCGGCTGGCGGAGGAAATCCTTTAACGCCCACGGTGTCAGGCGACC‑GCCTTGTTCAccc                    >  1:374577/1‑251 (MQ=255)                                                                                                                                                                                                                                tCAGACGGGCATTAACGATAGCGCCATTGTGCA‑TATCACCCTCTACCCACACCGGTGAGGTGAT‑AAACCGCTCCAGCAGATATCGTTCTGGCGGGCGCACATCGGGCTCGTAGACGGTAACGCCACCTTCACGCGGGCGCTTTTCGTAATTCATCAATTGGAGTTGGGCGCTACAGTAAAGGCCATACAGCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAAC‑GCCTTGTTCACCCTg                  <  1:608976/250‑1 (MQ=255)                                                                                                                                                                                                                                  aGACGGGCATTAACGATAGTGCCATTGTGCA‑TATCACCCTCGACCCACACCGGTGAGGTGAT‑AAACCGCTCCATCAGATAGCGTTCTGGCGGACGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGCCAGTAAAGGCCATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCCGGGTCAGGCGAAAAGCCT‑GTTCACCCTGCa                >  2:456249/1‑250 (MQ=255)                                                                                                                                                                                                                                  aGACGGGCATTAACGATAGCGCCATTGTGCA‑TATCACCCTCGACCCACACCGGTGAGGTGAT‑AAACCGCTCCATCAGATAGCGTTCTGGCGGGCGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAAC‑GCCTTGTTCACCCTGCaa               <  1:889389/251‑1 (MQ=255)                                                                                                                                                                                                                                    aCGGGCATTAACGATAGCGCCATTGTGCA‑TATCACCCTCGACCCACACCGGTGAGGTGAT‑AAACCGCTCCATCAGATAGCGTTCTGGCGGGCGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAAC‑GCCTTGTTCACCCTGCaaaa             <  1:948984/251‑1 (MQ=255)                                                                                                                                                                                                                                        gCATTAACGATAGCGCCATTGTGCA‑TATCACCCTCGACCCACACCGGTGAGGTGAT‑AAACCGCTCCATCAGATAGCGTTCTGGCGGGCGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAAC‑GCCTTGTTCACCCTGCAAAATATg         <  1:570391/251‑1 (MQ=255)                                                                                                                                                                                                                                            tAACGATAGCGCCATTGTGCA‑TATCACCCTCGACCCACACCGGTGAGGTGAT‑AAACCGCTCCATCAGATAGCGTTCTGGCGGGCGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTGAAAATCCTTTTACGCCAGCGGGGTCAGGCGAAC‑GCCTTTTTCACCCTGCCAAATATGCTgg     >  1:201596/1‑250 (MQ=255)                                                                                                                                                                                                                                            tAACGATAGCGCCATTGTGCA‑TATCACCCTAGACCCACACCGGTGAGGTGAT‑AAACCGCTCCATCAGATAGCGTTCTGGCGGGCGCACATCGGCCTCGTAGACGGTAACGCCACCTGCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAAC‑GCCTTGTTCACCCTGCAAAATATGCTg      <  2:806150/250‑1 (MQ=255)                                                                                                                                                                                                                                               cGATAGCGCCATTGTGCA‑TATCACCCTCGACCCACACCGGTGAGGTGAT‑AAACCGCTCCATCAGATAGCGTTCTGGCGGGCGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTGAAAATCCTTTACCGCCAACTGTGTCAAGCGAAA‑GGCTTGTTCACCCTGCCAAATATGCTGAgcg  >  1:989645/1‑251 (MQ=255)                                                                                                                                                                                                                                               cGATAGCGCCATTGTGCA‑TATCACCCTCGACCCACACCGGTGAGGTGAT‑AAACCGCTCCATCAGATAGCGTTCTGGCGGGCGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTGAAAATCCTTTAAAGCCAGCGGTGTCAGGCGACC‑GCCTTGTTCACCCCGCAAAATATGCTGAgcg  >  1:89592/1‑251 (MQ=255)                                                                                                                                                                                                                                               cGATAGCGCCATTGTGCA‑TATCACCCTCGACCCACACCGGTGAGGTGAT‑AAACCGCTCCATCAGATAGCGTTATGGCGGGCGCACATCGGCCTCGTAGACGGTAACGCCACCTTCCCGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTGAAAAACCTTTAAAGCCAACGGTGTCAGGCGAAC‑GACTTGTTCACCCTGCAAAATATgactag    >  1:536217/1‑244 (MQ=255)                                                                                                                                                                                                                                                gATAGCGCCATTGTGCA‑TATCACCCTCGACCCACACCGGTGAGGTGAT‑AAACCGCTCCATCAGATAGCGTTCTGGCGGGCGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCCGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACAcc                                                                              >  1:545067/1‑175 (MQ=255)                                                                                                                                                                                                                                                gATAGCGCCATTGTGCA‑TATCACCCTCGACCCACACCGGTGAGGTGAT‑AAACCGCTCCATCAGATAGCGTTCTGGCGGGCGCACATCGGCCTCGTAGACGGTAACGCCACCTGCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACAcc                                                                              <  2:545067/175‑1 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                        GTTGGCAAACCAGGCGTTGAGTTTTTCCAGCAACTGCGGGCGGCTGGCGACGTATTCCAGTTCGAAATCAAGCGAGGAGGCGTCGCCATTCTCCGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAATATCTATAGAAACCCACTTGAGCGGCGGA‑CGATAGT‑CGGGATGCGGTTTCAGACGGGCATTAACGATAGTGCCATTGTGCA‑TATCACCCTCGACCCACACCGGTGAGGTGAT‑AAACCGCTCCATCAGATAGCGTTCTGGCGGACGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAAA‑GCCTTGTTCACCCTGCAAAATATGCTGAGCG  >  NC_000913/65139‑65625

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑