Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 89,241 C→A intergenic (+209/‑393) cra → / → mraZ DNA‑binding transcriptional dual regulator Cra/DNA‑binding transcriptional repressor MraZ

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091389,2410CA97.0% 191.0 / ‑0.5 67intergenic (+209/‑393)cra/mraZDNA‑binding transcriptional dual regulator Cra/DNA‑binding transcriptional repressor MraZ
Reads supporting (aligned to +/- strand):  ref base C (1/1);  new base A (29/36);  total (30/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.25e-01

AATCTCTATCGCCGCGGCGTGCTCAGCCGTAGCTAAGCCGCGAACAAAAATACGCGCCAGGTGAATTTCCCTCTGGCGCGTAGAGTACGGGACTGGACATCAATATGCTTAAAGTAAATAAGACTATTCCTGACTATTATTGATAAATGCTTTTAAACCCGCCCGTTAATTAACTCACCAGCTGAAATTCACAATAATTAAGTGATATCGACAGCGCGTTTTTGCATTATTTTGTTACATGCGGCGATGAATTGCCGATTTAACAAACACTTTTCTTTGCTTTTGCGCAAACCCGCTGGCATCAAGCGCCACACAGACGTAACAAG‑GACTGTTAACCGGGGAAGATATGTCCTAAAATGCCGCTCGCGTCGCAAACTGACACTTTATATTTGCTGTGGAAAATAGTGAGTCATTTTAAAACGGTGATGACGATGAGGGATTTTTTCTTACAGCTATTCATAACGTTAATTTGCTTCGCACGTTGGA  >  NC_000913/88997‑89482
                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                  
aaTCTCTCTCGCCGCGGCGTGCTCAGCCGTAGCTAAGCCGCGAACAAAAATACGCGCCAGGTGAATTTCCCTCTGGCGCGTAGAGTACGGGAATGGACATCAATATCCTTAAAGTAAATAAGACTATTCCTGACTATTATTGATAAGTTCTTTTAAACCCGCCCGTTAATTAACTCACCAGCTGAAATTCACAATAATTAAGTGATATCGACAGCGCGTTTTTGCATTATTTTGTTACATGCAGAGATGa                                                                                                                                                                                                                                               <  2:145928/250‑1 (MQ=255)
     ctATCGCCGCGGCGTGCTCAGCCGTAGCTAAGCCGCGAACAAAAATACGCGCCAGGTGAATTTCCCTCTGGCGCGTAGAGTACGGGAATGGACATCAATATCCTTAAAATAAATAAGACTATTCCTGACTATTATTGATAAGTTCTTTTAAACCCGCCCGTTAATTAACTCACCAGCTGAAATTCACAATAATTAAGTGATATCGACAGCGCGTTTTTGCATTATTTTGTTAAATGCAGAGATGAATTGcc                                                                                                                                                                                                                                         >  2:753490/1‑251 (MQ=255)
        acgccgcGGCGTGCTCAGCCGTAGCTAAGCCGCGAACAAAAAGACGCGCCAGGTGAATTTCCCTCTGGCGCGTAGAGTACGGGAATGGACATCAATATCCTTAAAGTAAATAAGACTATTCCTGACTATTATTGATAAGTTCTTTTAAACCCGCCCGTTAATTAACTCACCAGCTGAAATTCACAATAATTAAGTGATATCGACAGCGCGTTTTTGCATTATTTGGTTACATGCAGAGATGAATTGCCGAt                                                                                                                                                                                                                                      <  2:138653/250‑1 (MQ=255)
         cgccgcGGCGTGCTCAGCCGTAGCTAAGCCGCGAACAAAAATACGCGCCAGGTGAATTTCCCTCTGGCGCGTAGAGTACGGGAATGGACATCAATATCCTTAAAGTAAATAAGACTATTCCTGACTATTATTGATAAGTTCTTTTAAACCCGCCCGTTAATTAACTCACCAGCTGAAATTCACAATAATTAAGTGATATCGACAGCGCGTTTTTGCATTATTTTGTTACATGCAGAGATGAATTGCCGAt                                                                                                                                                                                                                                      <  1:987486/250‑1 (MQ=255)
         cgccgcGGCGTGCTCAGCCGTAGCTAAGCCGCGAACAAAAATACGCGCCAGGTGAATTTCCCTCTGGCGCGTAGAGTACGGGAATGGACATCAATATCCTTAAAGTAAATAAGACTATTCCTGACTATTATTGATAAGTTCTTTTAAACCCGCCCGTTAATTAACTCACCAGCTGAAATTCACAATAATTAAGTGATATCGACAGCGCGTTTTTGCATTATTTTGTTACATGCAGAGATGAATTGCCGAt                                                                                                                                                                                                                                      <  1:291423/250‑1 (MQ=255)
          gccgcGGCGTGCTCAGCCGTAGCTAAGCCGCGAACAAAAATACGCGCCAGGTGAATTTCCCTCTGGCGCGTAGAGTACGGGAATGGACATCAATATCCTTAAAGTAAATAAGACTATTCCTGCCTATTATTGCTAAGTTCTTTTAAACCCGCCCGTTAATTAACTCACCAGCTGAAATTCACAATAATTAAGTGATATCGACAGCGCGTTTTTGCATTATTTTGTTACATGCAGAGATGAATTGCCGAttt                                                                                                                                                                                                                                    >  2:12922/1‑251 (MQ=255)
           ccgcGGCGTGCTCAGCCGTAGCTAAGCCGCGAACAAAAATACGCGCCAGGTGAATTTCCCTCTGGCGCGTAGAGTACGGGAATGGACATCAATATCCTTAAAGTAAATAAGACTATTCCTGACTATTATTGAGAAGTTCTTTTAAACCCGCCCGTTAATTAACTCACCAGCTGAAATTCACAATAATTAAGTGATATCGACAGCGCGTTTTTGCATTATTTTGTTACATGCAGAGATGAATTGCCGATTTa                                                                                                                                                                                                                                   <  1:606104/251‑1 (MQ=255)
             gcggcgTGCTCAGCCGTAGCTAAGCCGCGAACAAAAATACGCGCCAGGTGAATTTCCCTCTGGCGCGTAGAGTACGGGAATGGACATCAATATCCTTAAAGTAAATAAGACTATTCCTGACTATTATTGATAAGTTCTTTTAAACCCGCCCGTTAATTAACTCACCAGCTGAAATTCACAATAATTAAGTGATATCGACAGCGCGTTTTTGCATTATTTTGTTACATGCAGAGATGAATTGCCGATTTaac                                                                                                                                                                                                                                 >  1:229572/1‑251 (MQ=255)
              cggcgTGCTCAGCCGTAGCTAAGCCGCGAACAAAAATACGCGCCAGGTGAATTTCCCTCTGGCGCGTAGAGTACGGGAATGGACATCAATATCCTTAAAGTAAATAAGACTATTCCTGACTATTATTGATAAGTTCTTTTAAACCCGCCCGTTAATTAACTCACCAGCTGAAATTCACAATAATTAAGTGATATCGACAGCGCGTTTTTGCATTATTGTGTTACATGCAGAGATGAATTGCCGATTTaaca                                                                                                                                                                                                                                <  1:285426/251‑1 (MQ=255)
                gcgTGCTCAGCCGTAGCTAAGCCGCGAACAAAAATACGCGCCAGGTGAATTTCCCTCTGGCGCGTAGAGTACGGGAATGGACATCAATATCCTTAAAGTAAATAAGACTATTCCTGACTATTATTGATAAGTTCTTTTAAACCCGCCCGTTAATTAACTCACCAGCTGAAATTCACAATAATTAAGTGATATCGACAGCGCGTTTTTGCATTATTTTGTTACATGCAGAGATGAATTGCCGATTTaacaag                                                                                                                                                                                                                              <  1:458135/251‑2 (MQ=255)
                     cTCAGCCGTAGCTAAGCCGCGAACAAAAATACGCGCCCGGTGAATTTCCCTCTGGCGCGTAGAGTACGGGAATGGACATCAATATCCTTAAAGTCAATAAGACTATTACTGACTATTCTTGATAAGTTCTTTTAAACCCGCCCGTTAATTAACTCACCAGCTGAACTTCACAATAATTAAGTGATATCGACAGCGCGTTTTTGCATTATTTTGTTACATGAAGAGATGAATTGCCGATTTaacacgaactt                                                                                                                                                                                                                         >  2:738021/1‑244 (MQ=255)
                       cAGCCGTAGCTAAGCCGCGAACAAAAATACGCGCCAGGTGAATTTCCCTCTGGCGCGTAGAGTACGGGAATGGACATCAATATCCTTAAAGTAAATAAGACTATTCCTGACTATTATTGATAAGTTCTTTTAAACCCGCCCGTTAATTAACTCACCAGCTGAAATTCACAATAATTAAGTGATATCGACAGCGCGTTTTTGCATTATTTTGTTACATGCAGAGATGAATTGCCGATTTAACAAGCACtttt                                                                                                                                                                                                                       <  1:607162/251‑1 (MQ=255)
                         gCCGTAGCTAAGCCGCGAACAAAAATACGCGCCAGGTGAATTTCCCTCTGGCGCGTAGAGTACGGGAATGGACATCAATATCCTTAAAGTAAATAAGACTATTCCTGACTATTATTGATAAGTTCTTTTAAACCCGCCCGTTAATTAACTCACCAGCTGAAATTCACAATAATTAAGGGATATCGACAGCGCGTTTTTGCATTATTTTGTTACATGCAGAGATGAATTGCCGATTTAACAAGCACTTTTCt                                                                                                                                                                                                                     <  2:452821/251‑1 (MQ=255)
                           cGTAGCTAAGCCGCGAACAAAAATACGCGCCAGGTGAATTTCCCTCTGGCGCGTAGAGTACGGGAATGGACATCAATATCCTTAAAGTAAATAAGACTATTCCTGACTATTATTGATAAGTTCTTTTAAACCCGCCCGTTAATTAACTCACCAGCTGAAATTCACAATAATTAAGTGATATCGACAGCGCGTTTTTGCATTATTTTGTTACATGCAGAGATGAATTGCCGATTTAACAAGCACTTTTCttt                                                                                                                                                                                                                   <  1:221974/251‑1 (MQ=255)
                               acTAAGCTGCGCACAAAAATACACGCCAGTTGCATTTCCCTCTGGCGCGTAGTGTACGGGAATGAACATCACTATCCTTAAAGTAAATAAGACTTTTCCTGACTATTATTGATAAGTTCTTTTAAACCAGCCCGTTAATTAACTCACCAGCTGAAATTCACAATAATTAAGTGATATCGACGGCGCGTTTTTGCATTATTTTGTTACATGCAGAGATGAATTGACGATTAAACAAGAACTTTTCTTTGCtt                                                                                                                                                                                                               >  2:736325/2‑251 (MQ=255)
                                  aaGCCGCGAACAAAAATACGCGCCAGGTGAATTTCCCTCTGGCGCGTAGAGTACGGGAATGGACATCAATATCCTTAAAGTAAATAAGACTATTCCTGACTATTATTGATAAGTTCTTTTAAACCCGCCCGTTAATTAACTCACCAGCTGAAATTCACAATAATTAAGTGATATCGACAGCGCGTTTTTGCATTATTTTGTTACATGCAGAGATGAATTGCCGATTTAACAAGCACTTTTCTTTGCtttt                                                                                                                                                                                                             <  1:756358/250‑1 (MQ=255)
                                  aaGCCGCGAACAAAAATACGCGCCAGGTGAATTTCCCTCTGGCGCGTAGAGTACGGGAATGGACATCAATATCCTTAAAGTAAATAAGACTATTCCTGACTATTATTGATAAGTTCTTTTAAACCCGCCCGTTAATTAACTCACCAGCTGAAATTCACAATAATTAAGTGATATCGACAGCGCGTTTTTGCATTATTTTGTTACATGCAGAGATGAATTGCCGATTTAACAAGCACTTTTCTTTGCttt                                                                                                                                                                                                              <  2:135887/249‑1 (MQ=255)
                                  aaGCCGCGAACAAAAATACGCGCCAGGTGAATTTCCCTCTGGCGCGTAGAGTACGGGAATGGACATCAATATCCGTAAAGTAAATAAGACTATTCCTGACTATTATTGATAAGTTCTTTTAAACCCGCCCGTTAATTAACTCACCAGCTGAAATTCACAATAATTAAGTGATAGCGACAGCGCGTTTTTGCATTATTTTGTTACATGCAGAGATGAATTGCCGATTTAACAAGCACTTTTCTTTGCTTTTg                                                                                                                                                                                                            <  1:890448/251‑1 (MQ=255)
                                                aaTACGCGCCAGGTGAATTTCCCTCTGGCGCGTAGAGTACGGGAATGGACATCAATATCCTTAAAGTAAATAAGACTATTCCTGACTATTATTGATAAGTTCTTTTAAACCCGCCCGTTAATTAACTCACCAGCTGAAATTCACAATAATTAAGTGATATCGACAGCGCGTTTTTGCATTATTTTGTTACATGCAGAGATGAATTGCCGATTTAACAAGCACTTTTCTTTGCTTTTGCGCAAACCCGCTgg                                                                                                                                                                                              <  1:3046/251‑1 (MQ=255)
                                                                  ttCCCTCTGGCGCGTAGAGTACGGGAATGGACATCAATATCCTTAAAGTAAATAAGACTATTCCTGACTATTATTGATAAGTTCTTTTAAACCCGCCCGTTAATTAACTCACCAGCTGAAATTCACAATAATTAAGTGATATCTACAGCGCGTTTTTGCATTATTTTGTTACATGCAGAGATGAATTGCCGATTTAACAAGCACTTTTCTTTGCTTTTGCGCATACCCGCTGGCATCAAGCGCCGCACAg                                                                                                                                                                             <  1:753490/250‑1 (MQ=255)
                                                                    cccTCTGGCGCGTAGAGTACGGGAATGGACATAAATATCCTTAAAGTAAATAAGACTATTCCTGACTATTATTGATAAGTTCTTTTAAACCCGCCCGTTAATTAACTCACCAGCTGAAATTCACAATAATTAAGTGATATCGACAGCGCGTTTTTGCATTATTTTGTTACATGCAGAGATGAATTGCCGATTTAACAAGCACTTTTCTTTGCTTTTGCGCAAAGCCGCTGGCATCAAGCGCCACACAGACg                                                                                                                                                                          >  2:680917/1‑251 (MQ=255)
                                                                       tctGGCGCGTAGAGTACGGGAATGGAAATCAATATCCTTAAAGTAAATAAGACTATTCCTGACTATTATTGATAAGTTCTTTTAAACCCGCCCGTTAATTAACTCACCAGCTGAAATTCACAATAATTAAGTGATATCGACAGCGCGTTTTTGCATTATTTTGTTACATGCAGAGATGAATTGCCGATTTAACAAGCACTTTTCTTTGCTTTTGCGCAAACCCGCTGGCATCAAGCGCCACCCAGACgca                                                                                                                                                                        >  2:905929/1‑248 (MQ=255)
                                                                                  gagTACGGGAATGGACATCAATATCCTTAAAGTAAATAAGACTATTCCTGACTATTATTGATAAGTTCTTTTAAACCCGCCCGTTAATTAACTCACCAGCTGAAATTCACAATAATTAAGTGATATCGACAGCGCGTTTTTGCATTATTTTGTTACATGCAGAGATGAATTGCCGATTTAACAAGCACTTTTCTTTGCTTTTGCGCAAACCCGCTGGCATCAAGCGCCACACAGACGCAACAGG‑GACTGtt                                                                                                                                                           <  1:986919/251‑1 (MQ=255)
                                                                                          gaatggaaataaatagccTTAAAGTAAATAAGACTATTCCTGACTATTATTGATAAGTTCTTTAAAACCCGCCCGTGAATTAACTCACCAGCTGAAATTCACAATAATTAAGTGATATCGACAGCGCGTTTTTGCATTATTTTGTTACATGCAGAGATGAAGTGCCGATTTAACAAGCACTTTTCTTTGCTTTTGCGCAAACCCGCTGGCATCAAGCGCAACACAGACGCAACATG‑GACTGCTAACCgggt                                                                                                                                                   <  2:151573/234‑2 (MQ=255)
                                                                                             tGGACATCAATATCCTTAAAGTAAATAAGACTATTCCTGACTATTATTGATAAGTTCTTTTAAACCCGCCCGTTAATTAACTCACCAGCTGAAATTCACAATAATTAAGTGATATCGACAGCGCGTTTTTGCATTATTTTGTTACATGCAGAGATGAATTGCCGATTTAACAAGCACTTTTCTTTGCTTTTGCGCAAACCCGCTGGCATCAAGCGCCACACAGACGCAACAGG‑GACTGTTAACCGGGGa                                                                                                                                                  >  1:719610/1‑249 (MQ=255)
                                                                                                  aTCAATATCCTTAAAGTAAATAAGACTATTCCTGACTATTATTGATAAGTTCTTTTAAACCCGCCCGTTAATTAACTCACCAGCTGAAATTCACAATAATTAAGTGATCTCGACAGCGCGTTTTTGCATTATTTTGTTACATGCAGAGATGAATTGCCGATTTAACAAGCACTTTTCTTTGCTTTTGCGCAAACCCGCTGGCATCAAGCGCCACACAGAAGCAACAGG‑GACTGTTAACCGGGGAAGATATg                                                                                                                                           >  2:317366/1‑251 (MQ=255)
                                                                                                  aTCAATATCCTTAAAGTAAATAAGACTATTCCTGACTATTATTGATAAGTTCTTTTAAACCCGCCCGTTAATTAACTCACCAGCTGAAATTCACAATAATTAAGTGATATCGACAGCGCGTTTTTGCATTATTTTGTTACATGCAGAGATGAATTGCCGATTTAACAAGCACTTTTCTTTGCTTTTGCGCAAACCCGCTGGCATCAAGCGCCACACAGACGCAACAGG‑GACTGTTAACCGGGGAAGATATg                                                                                                                                           >  1:441213/1‑251 (MQ=255)
                                                                                                      atatCCTTAAAGTAAATAAGACTATTCCTGACTATTATTGATAAGTTCTTTTAAACCCGCCCGTTAATTAACTCACCAGCTGAAATTCACAATAATTAAGTGATATCGACAGCGCGTTTTTGCATTATTTTGTTACATGCAGAGATGAATCGCCGATTTAACAAGCACTTTTCTTTGCTTTTGCGCAAACCCGCTGGCATCAAGCGCCACACAGACGCAACAGG‑GACTGTTAACCGGGGAAGATATGTCCt                                                                                                                                       >  1:286547/1‑251 (MQ=255)
                                                                                                        atccTTAAAGTAAATAAGACTATTCCTGACTATTATTGATAAGTTCTTTTAAACCCGCCCGTTAATTAACTCACCAGCTGAAATTCACAATAATTAAGTGATATCGACAGCGCGTTTTTGCATTATTTTGTTACATGCAGAGATGAATTGCCGATTTAACAAGCACTTTTCTTTACTTTTGCGCAAACCCGCTGGCATCAAGCGCCACACAGACGCAACAGG‑GACTGTTAACCGGGGAAAATATGTCCTa                                                                                                                                      >  2:884342/4‑250 (MQ=255)
                                                                                                              aaaGTAAATAAGTCTATTCCTGACTATTATTGATAAGTTCTTTTAAACCCGCCCGTTAATTAACTCACCAGCTGAAATTCACAATAATTAAGTGATATCGACAGCGCGTTTTTGCATTATTTTGTTACATGCAGAGATGAATTGCCGATTTAACAAGCACTTTTCTTTGCTTTTGCGCAAACCCGCTGGCATCAAGCGCCACACAGACGCAACAGG‑GACTGTTAACCGGGGAAGATATGTCCTAAAATGcc                                                                                                                               <  2:952396/251‑1 (MQ=255)
                                                                                                                   aaaTAAGACTATTCCTGACTATTATTGATAAGTTCTTTTAAACCCGCCCGTTAATTAACTCACCAGCTGAAATTCACAATAATTAAGTGATATCGACAGCGCGTTTTTGCATTATTTTGTTACATGCAGAGATGAATTGCCGATTTAACAAGCACTTTTCTTTGCTTTTGCGCAAACCCGCTGGCATCAAGCGCCACACAGACGCAACAGG‑GACTGTTAACCGGGGAAGATATGTCCTAAAATGCCGCTc                                                                                                                           <  1:945380/250‑1 (MQ=255)
                                                                                                                       aaGACTATTCCTGACTATTATTGATAAGTTCTTTTAAACCCGCCCGTTAATTAACTCACCAGCTGAAATTCACAATAATTAAGTGATATCGACAGCGCGTTTTTGCATTATTTTGTTACATGCAGAGATGAATTGCCGATTTAACAAGCACTTTTCTTTGCTTTTGCGCAAACCCGCTGGCATCAAGCGCCACACAGACGCAACAGG‑GACTGTTAACCGGGGAAGATATGTCCTAAAATGCCGCTCGCGTc                                                                                                                      <  1:3094/251‑1 (MQ=255)
                                                                                                                       aaGACTATTCCTGACTATTATTGATAAATGCTTTTAAACCCGCCCGTTAATTAACTCACCAGCTGAAATTCACAATAATTAAGTGATATCGACAGCGCGTTTTTGCATTATTTTGTTACATGCGGCGATGAATTGCCGATTTaaca                                                                                                                                                                                                                                <  2:306971/146‑1 (MQ=255)
                                                                                                                       aaGACTATTCCTGACTATTATTGATAAATGCTTTTAAACCCGCCCGTTAATTAACTCACCAGCTGAAATTCACAATAATTAAGTGATATCGACAGCGCGTTTTTGCATTATTTTGTTACATGCGGCGATGAATTGCCGATTTaaca                                                                                                                                                                                                                                >  1:306971/1‑146 (MQ=255)
                                                                                                                            tATTCCTGCCTATTATTGATACGTTCTTTTAAACCCGCCCGTTAATTAACTCACCAGCTTAAATTCACAATAATTAAGTGATATCGACAGCGCGATTTTGCATTATTTTGTTACATGCAGAGATGAATTGCCGCTTTAACAAGCACTTTTCTTTGCTTTTGCGAAAACCAGCTGGCATCAACCGCCACACTGACGCAACAGG‑GACTGTCAACCGGGGAAGCTATGTCCTATATGGCCGCTCGCGTCGCaa                                                                                                                  >  2:733099/1‑250 (MQ=255)
                                                                                                                             aTTCCTGACTATTATTGATAAGTTCTTTTAAACCCGCCCGTTAATTAACTCACCAGCTGAAATTCACAATAATTAAGTGATATCGACAGCGCGTTTTTGCATTATTTTGTTACATGCAGAGATGAATTGCCGATTTAACAAGCACTTTTCTTTGCTTTTGCGCAAACCCGCTGGCATCAAGCGCCACACAGACGCAACAGG‑GACTGTTAACCGGGGAAGATATGTCCTAAAATGCCGCTCGCGTCGCaaa                                                                                                                 <  1:345035/250‑1 (MQ=255)
                                                                                                                               tCCTGACTATTATTGATAAGTTCTTTTAAACCCGCCCGTTAATTAACTCACCAGCTGAAATTCACAATAATTAAGTGATATCGACAGCGCGTTTTTGCATTATTTTGTTACATGCAGAGATGAATTGCCGATTTAACAAGCACTTTTCTTTGCTTTTGCGCAAACCCGCTGGCATCAAGCGCCACACAGACGCAACAGG‑GACTGTTAACCGGGGAAGATATGTCCTAAAATGCCGCTCGCGTCGCAAACt                                                                                                               <  1:974290/250‑1 (MQ=255)
                                                                                                                                       attattGATAAGTTCTTTTAACCCCGCCCGTTAATTAACTCACCAGCTGAAATTCACAATAATTAAGTGTTATCGACAGCGCGTTTTTGCATTATTTTGTTACATGCAGAGATGAATTGCCGATTTAACAAGCACTTTTCTTTGCTTTTGCGCAAACCCGCTGGCATCAAGCGCCACACAGACGCAACAGG‑GACTGTTAACCGGGGAAGATATGTCCTAAAATGCCGCTCGCGTCGCAAACTGACACtt                                                                                                        <  1:583100/249‑1 (MQ=255)
                                                                                                                                       attattGATAAGTTCTTTTAAACCCGCCCGTTAATTAACTCACCAGCTGAAATTCACAATAATTAAGTGATATCGACAGCGCGTTTTTGCATTATTTTGTTACATGCAGAGATGAATTGCCGATTTAACAAGCACTTTTCTTTGCTTTTGCGCAAACCCGCTGGCATCAAGCGCCACACAGACGCAACAGG‑GACTGTTAACCGGGGAAGATATGTCCTAAAATGCCGCTCGCGTCGCAAACTGACACttt                                                                                                       <  1:758860/250‑1 (MQ=255)
                                                                                                                                       attattGATAAGTTCTTTTAAACCCGCCCGTTAATTAACTCACCAGCTGAAATTCACAATAATTAAGTGATATCGACAGCGCGTTTTTGCATTATTTTGTTACATGCAGAGATGAATTGCCGATTTAACAAGCACTTTTCTTTGCTTTTGCGCAAACCCGCTGGCATCAAGCGCCACACAGACGCAACAGG‑GACTGTTAACCGGGGAAGATATGTCCTAAAATGCCGCTCGCGTCGCAAACTGACACtt                                                                                                        <  2:654191/249‑1 (MQ=255)
                                                                                                                                            tGATAAGTTCTTTTAAACCCGCCCGTTAATTAACTCACCAGCTGAAATTCACAATAATTAAGTGATATCGACAGCGCGTTTTTGCATTATTTTGTTACATGCAGAGATGAATTGCCGATTTAACAAGCACTTTTCTTTGCTTTTGCGCAAACCCGCTGGCATCAAGCGCCACACAGACGCAACAGG‑GACTGTTAACCGGGGAAGATATGTCCTAAAATGCCGCTCGCGTCGCAAACTGACACTTTATAtt                                                                                                  <  1:993345/250‑1 (MQ=255)
                                                                                                                                              ataagttcTTTTAAACCCGCCCGTTAATTAACTCACCAGCTGAAATTCACAATAATTAAGTGATATCGACAGCGCGTTTTTGCATTATTTTGTTACATGCAGAGATGAATTGCCGATTTAACAAGCCCTTTTCTTTGCTTTTGCGCAAACCCGCTGGCATCAAGCGCCACACAGACGCAACAGG‑GACTGTTAACCGGGGAAGATATGTCCTAAAATGCCGCTCGCGTCGCAAACTGACACTTTATATTTGc                                                                                               >  1:750632/8‑251 (MQ=255)
                                                                                                                                                 atgtcTTTTAAATCCGCCTGTGGATTAACTACCCAGTTGAAATCCCCAATATATCAGTGATAACGACATCTCGTGTTTGCATTATTTAGTTCCATGCAGAGAGGAATTGCCGATTTAACAAGCACTTTTCTTTGCTTTTGCGCAAACCCGCTGGAATCAAGCGCCACACAGCCTCAACAGG‑GACTGTTAACCGGTTAAGATAGTTCCTAAAATGCCGCTCGCGACGCCAACTGACCCTTTATAGTTGCtgt                                                                                            <  2:727491/247‑1 (MQ=255)
                                                                                                                                                 agttcTTTTAAACCCGCACGTTAATTAACTCACCAGCTGAAATTCACAATAATTAAGTGATATCGACAGCGCGTTTTTGCATTATTTTGTTACATGCAGAGATGAATTGCCGATTTAACAAGCACTTTTCTTTGCTTTTGCGCAAACCCGCTGGCATCAAGCGCCACACAGACGCAACAGG‑GACTGTTAACCGGGGAAGATATGTCATAAAATGCCGCTCGCGTCGCACACTGGCACTTTATATTTGCtgt                                                                                            >  1:765948/5‑251 (MQ=255)
                                                                                                                                                    tcTTTTAAACCCGCCCGTTAATTAACTCACCAGCTGAAATTCAAAATAATTATGTGATATCGACAGCGCGTTTTTGCATTATTTTGTTACATGCAGAGATGAATTGCCGATTTAACAAGCACTTTTCTTTGCTTTTGCGCAAACCCGCTGGCATCAAGCGCCACACAGACGCAACAGG‑GACTGTTAACCGGGGAAGATATGTCCTAAAATGCCGCTCGCGTCGCAAACTGACACTTTATATTTGCTGTgg                                                                                          >  1:362319/2‑250 (MQ=255)
                                                                                                                                                        ttAAACCCGCCCGTTAATTAACTCACCAGCTGAAATTCACAATAATTAAGTGATATCGACAGCGCGTTTTTGCATTATTTTGTTACATGCAGAGATGAATTGCCGATTTAACAAGCACTTTTCTTTGCTTTTGCGCACACCCGCTGGCATCAAGCGCCACACAGACGCAACAGG‑GACTGTTAACCGGGGAAGATATGTCCTAAAATGCCGCTCGCGTCGCAAACTGACACTTTATATTTGCTGTGGAAAAt                                                                                     >  1:891368/1‑251 (MQ=255)
                                                                                                                                                              ccgccctTTAATTAACTCACCAGCTGAAATTCACAATAATTAAGTGATATCGACAGCGCGTTTTTGCATTATTTTGTTACATGCAGAGATGAATTGCCGATTTAACAAGCACTTTTCTTTGCTTTTGCGCAAACCCGCTGGCATCAAGCGCCACGCAGACGCAACAGG‑GACTGTTAACCGGGGAAGATATGTCCTAAAATGCCGCTCGCGTCGCAAACTGACACTTTATATTTGCTGTGGAAAATAGTGAg                                                                               <  1:482647/251‑1 (MQ=255)
                                                                                                                                                                 accgTTAATTAACTCACCAGCTGAAATTCACAATAATTAAGTGATATCGACAGCGCGTTTTTGCATTATTTTGTTACATGCAGAGATGAATTGCCGATTTAACAAGCACTTTTCTTTGCTTTTGCGCAAACCCGCTGGCATCAAGCGCCACACAGACGCAAACGG‑GACTGTTAACAGGGGAAGATATGTCCTAAAATGCCGCTCGCGTCGCAAACTGACACTTTATATTTGCTGTGGAAAATAGTGCGTc                                                                             >  2:104887/2‑250 (MQ=255)
                                                                                                                                                                  ccgTTAATTAACTCACCAGCTGAAATTCACAATAATTAAGTGATATCGACAGCGCGTTTTTGCATTATTTTGTTACATGCAGAGATGAATTGCCGATTTAACAAGCACTTTTCTTTGCTTTTGCGCAAACCCGCTGGCATCAAGCGCCACACAGACGCAACAGG‑GACTGTTAACCGGGGAAGATATGTCCTAAAATGCCGCTCGCGTCGCAAACTGACACTTTATATTTGCTGTGGAAAATAGTGAGTCAt                                                                           <  1:110583/251‑1 (MQ=255)
                                                                                                                                                                         ttaaCTCACCATCTGAAATTCACAATAATTAAGTGATATCTACAGCGCGTTTTTGCATTATTTTGTTACATGCAGAGATGAATTGCCGATTTAACAAGCACTTTTCTTTGCTTTTGCGCAAACCCGCTGGCATCAAGCGCCACACAGACGCAACAGG‑GACTGTTAACCGGGGCAGATATGTCCTAAAATGCCGCTCGCGTCGCAAACTGACACTTTATATTTGCTTTGGAAAATAGGGAGTCATTATaaa                                                                     >  2:710039/1‑250 (MQ=255)
                                                                                                                                                                               cACCAGCTGAAATTCACAATAATTAAGTGATATCGACAGCGCGGTTTTGCATTATTTTGTTACATGCAGAGATGAATTGCCGATTTAACAAGCACTTTTCTTTGCTTTTGCGCAAACCCGCGGGCATCAAGCGCCACACAGACGCAACAGG‑GACTGTTAACCGGGGAAGATATGTCCTAAAATGCCGCTCTCGTCGCAAACTGACACTTGATATTTGCTGTGGAAAATAGTGAGTCATTTTAAAACGGtga                                                              <  1:489502/251‑1 (MQ=255)
                                                                                                                                                                                aCCAGCTGTAATTCATACAAATTCAGTGATATCGACAGAGCGTTGTTGCATTTTTGTGTAACATGCAGAGATGAATTGCCGAATTAACCATCACTCTTCTTTGCTTTTGCTCAAACTCGCTGGCATCAAGCGCCACACAGACGCAA‑ATGTGACTTTTAATCGTGTAAGATAAGTACTAAAATGCCGCTCGAGACGCAAACTGACACTTTATATTTGCTGTGGAAAATATTGAGTCATTTTAATACGGtgat                                                             <  2:589684/251‑1 (MQ=255)
                                                                                                                                                                                  cAGCTGAAATTCACAATAATTAAGTGATATCGACAGCGCGTTTTTGCATTATTTTGTTACATGCAGAGATGAATTGCCGATTTAACAAGCACTTTTCTTTGCTTTTGCGCAAACCCGCTGGCATCAAGCGCCACACAGACGCAACAGG‑GACTGTTAACCGGGGAAGATATGTCCTAAAATGCCGCTCGCGTCGCAAACTGACACTTTATATTTGCTGTGGAAAATAGTGAGTCATTTTAAAACGGtgatga                                                           <  1:425993/251‑1 (MQ=255)
                                                                                                                                                                                   aGCTGAAATTCACAATAATTAAGTGATATCGACAGCGCGTTTTTGCATTATTTTGTTACATGCAGAGATGAATTGCCGATTTAACAAGCACTTTTCTTTGCTTTTGCGCAAACCCGCTGGCATCAAGCGCCACACAGACGCAACAGG‑GACTGTTAACCGGGGAAGATATGTCCTAAAATGCCGCTCGCGTCGCAAACTGACACTTTATATTTGCTGTGGAAAATAGTGAGTCATTTTAAAACGGTGATGAc                                                          >  2:193809/1‑251 (MQ=255)
                                                                                                                                                                                      tGAAATTCACAATAATTAAGTGATATCGACAGCGCGTTTTTGCATTATTTTGTTACATGCAGAGATGAATTGCCGATTTAACAAGCACTTTTCTTTGCTTTTGCGCAAACCCGCTGGCATCAAGCGCCACACAGACGCAACAGG‑GACTGTTAACCGGGGAAGATATGTCCTAAAATGCCGCTCGCGTCGCAAACTGACACTTTATATTTGCTGTGGAAAATAGTGAGTCATTTTAAAACGGTGATGACGAt                                                       >  1:158666/1‑251 (MQ=255)
                                                                                                                                                                                          aTTCACAATAATTAAGGGATATCGACAGCGCGTTTTTGCATTATTTTGTTACATGCAGAGATGAATTGCCGATTTAACAAGCACTTTTCTTTGCTTTTGCGCAAACCCGCTGGCATCAAGCGCCACACAGACGCAACAGG‑GACTGTTAACCGGGGAAGATATGTCCTAAAATGCCGCTCGCGTCGCAAACTGACACTTTATATGTGCTGTGGAAAATAGTGAGTCATTTTAAAACGGTGATGACGATGAgg                                                   <  2:224965/251‑1 (MQ=255)
                                                                                                                                                                                            tCACAATAATTAAGTGATATCGACAGCGCGTTTTTGCATTATTTTGTTACATGCAGAGATGAATTGCCGATTTAACAAGCACTTTTCTTTGCTTTTGCGCAAACCCGCTGGCATCAAGCGCCACACAGACGCAACAGG‑GACTGTTAACCGGGGAAGATATGTCCTAAAATGCCGCTCGCGTCGCAAACTGACACTTTATATTTGCTGTGGAAAATAGTGAGTCATTTTAAAACGGTGATGACGATGAgggg                                                 >  1:356665/1‑250 (MQ=255)
                                                                                                                                                                                                aataatTAAGTGATATCGACAGCGCGTTTTTGCATTATTTTGTTACATGCAGAGATGAATTGCCGATTTAACAAGCACTTTTCTTTGCTTTTGCGCAAACCCGCTGGCATCAAGCGCCACACAGACGCAACAGG‑GACTGTTAACCGGGGAAGATATGTCCTAAAATGCCGCTCGCGTCGCAAACTGACACTTTATATTTGCTGTGGAAAATAGTGAGTCATTTTAAAACGGAGATGACGATGAGGGAtttt                                             >  1:356970/1‑251 (MQ=255)
                                                                                                                                                                                                    atTAAGTGATATCGACAGCGCGTTTTTGCATTATTTTGTTACATGCAGAGATGAATTGCCGATTTAACAAGCACTTTGCTTTGCTTTTGCGCAAACCCGCTGGCATCAAGCGCCACACAGACGCAACAGG‑GACTGTTAACCGGGGAAGATATGTCCTAAAATGCCGCTCGCGTCGCAAACTGACACTTTATATTTGCTGTGGAAAATAGTGAGTCATTTTAAAACGGTGATGACGATGAGGGATTTTTTCt                                         <  1:188320/251‑1 (MQ=255)
                                                                                                                                                                                                                cGACAGCGCGTTTTTGCATTATTTTGTTACATGCAGAGATGAATTGCCGATTTAACAAGCACTTTTCTTTGCTTTTGCGCAAACCCGCTGGCATCAAGCGCCACACAGACGCAACAGG‑GACTGTTAACCGGGGAAGATATGTCCTAAAATGCCGCTCGCGTCGCAAACTGACACTTTATATTTGCTGTGGAAAATAGTGAGTCATTTTAAAACGGTGATGACGATGAGGGATTTTTTCTTACAGCTATTc                              <  1:439555/250‑1 (MQ=255)
                                                                                                                                                                                                                 gACAGCGCGTTTTTGCATTATTTTGTTAAATGCAGAGATGAATTGCCGATTTAACAAGCACTTTTCTTTGCTTTTGCGCAAACCCGCTGGCATCAAGCGCCACACAGACGCAACAGG‑GACTGTTAACCGGGGAAGATATGTCCTAAAATGCCGCTCGCGTCGCAAACTGACACTTTATATTTGCTGTGGAAAATAGTGAGTCATTTTAAAACGGTGATGACGATGAGGGATTTTTTCTTACAGCTATTCAt                            >  2:620122/1‑251 (MQ=255)
                                                                                                                                                                                                                       gcgTTTTTGCATTATTTTGTTACATGCAGAGATGAATTGCCGATTTAACAAGCACTTTTCTTTGCTTTTGCGCAAACCCGCTGGCATCAAGCGCCACACAGACGCAACAGG‑GACTGTTAACCGGGGAAGATATGTCCTAAAATGCCGCTCGCGTCGCAAACTGACACTTTATATTTGCTGTGGAAAATAGTGAGTCATTTTAAAACGGTGATGACGTTGAGGGATTTTTTCTTACAGCTATTCATAACGtt                      >  2:489088/1‑251 (MQ=255)
                                                                                                                                                                                                                         gTTTTTGCATTATTTTGTTACATGCAGAGATGAATTGCCGATTTAACAAGCACTTTTCTTTGCTTTTGCGCAAACCCGCTGGCATCAAGCGCCACACAGACGCAACAGG‑GACTGTTAACCGGGGAAGATATGTCCTAAAATGCCGCTCGCGTCGCAAACTGACACTTTATATTTGCTGTGGAAAATAGTGAGTCATTTTAAAACGGTGATGACGATGAGGGATTTTTTCTTACAGCTATTCATAACGTTa                     >  1:582778/1‑250 (MQ=255)
                                                                                                                                                                                                                                      tttGTTACATGCAGAGATGAATTGCCGATTTAACAAGCACTTTTCTTTGCTTTTGCGCAAACCCGCTGGCATCAAGCGCCACACAGACGCAACAGG‑GACTGTTAACCGGGGAAGATATGTCCTAAAATGCCGCTCGCGTCGCAAACTGACACTTTATATTTGCTGTGGAAAATAGTGAGTCATTTTAAAACGGTGATGACGATGAGGGATTTTTTCTTACAGCTATTCATAACGTTAATTTGCTGCGCACg       >  1:532500/1‑251 (MQ=255)
                                                                                                                                                                                                                                      tttGTTACATGCAGAGATGAATTGCCGATTTAACAAGCACTTTTCTTTGCTTTTGCGCAAACCCGCTGGCATCAAGCGCCACACAGACGCAACAGG‑GACTGTTAACCGGGGAAGATATGTCCTAAAATGCCGCTCGCGTCGCAAACTGACACTTTATATTTGCTGTGGAAAATAGTGAGTCATTTTAAAACGGTGATGACGATGAGGGATTTTTTCTTACAGCTATTCATAACGTCAATTTGCTGCGCACg       >  1:878530/1‑251 (MQ=255)
                                                                                                                                                                                                                                       ttGTTACATGCAGAGATGAATTGCCGATTTAACAAGCACTTTTCTTTGCTTTTGCGCAAACCCGCTGGCATCAAGCGCCACACAGACGCAACAGG‑GACTGTTAACCGGGGAAGATATGTCCTAAAATGCCGCTCGCGTCGCAAACTGACACTTTATATTTGCTGTGGAAAATAGTGAGTCATTTTAAAACGGTGATGACGATGAGGGATTTTTTCTTACAGCTATTCATAACGTTAATTTGCTGCGCACGt      >  1:79315/1‑251 (MQ=255)
                                                                                                                                                                                                                                            aCATGCAGAGATGAATGTCCGATTTAACAAGCACTGTTCTTTGTTTTTGCGCAAACACGCTGGCATCAAGCGTCACTCAGACGCAACAGG‑GACTGTTAACCGGGGAAGATATGTCCTAAAATGCTGCTCGCGTCGCAAACTGACACTGTATATTTGCTGTTTAAAATAGTGAGTCATTTTAAAACGGTTATGGCGATTAGGGATTTTTCCTTACAGCTATTCATAACGTTAATTTGCTGCGCACGTTGGa  <  1:677588/250‑1 (MQ=255)
                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                  
AATCTCTATCGCCGCGGCGTGCTCAGCCGTAGCTAAGCCGCGAACAAAAATACGCGCCAGGTGAATTTCCCTCTGGCGCGTAGAGTACGGGACTGGACATCAATATGCTTAAAGTAAATAAGACTATTCCTGACTATTATTGATAAATGCTTTTAAACCCGCCCGTTAATTAACTCACCAGCTGAAATTCACAATAATTAAGTGATATCGACAGCGCGTTTTTGCATTATTTTGTTACATGCGGCGATGAATTGCCGATTTAACAAACACTTTTCTTTGCTTTTGCGCAAACCCGCTGGCATCAAGCGCCACACAGACGTAACAAG‑GACTGTTAACCGGGGAAGATATGTCCTAAAATGCCGCTCGCGTCGCAAACTGACACTTTATATTTGCTGTGGAAAATAGTGAGTCATTTTAAAACGGTGATGACGATGAGGGATTTTTTCTTACAGCTATTCATAACGTTAATTTGCTTCGCACGTTGGA  >  NC_000913/88997‑89482

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑