Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	89,103	G→C	intergenic (+71/‑531)	cra → / → mraZ	DNA‑binding transcriptional dual regulator Cra/DNA‑binding transcriptional repressor MraZ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	89,103	0	G	C	100.0%	237.5 / NA	68	intergenic (+71/‑531)	cra/mraZ	DNA‑binding transcriptional dual regulator Cra/DNA‑binding transcriptional repressor MraZ
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (25/43);  total (25/43)

TCGACTTCTTACAGTGTCCGGTGCTGGCAGTGGCTCAACGTCACCGCGATGTCGCAGAGCGTGTGCTGGAGATTGTCCTGGCAAGCCTGGACGAACCGCGTAAGCCAAAACCTG‑GTTTAACGCGCATTAAACGTAATCTCTATCGCCGCGGCGTGCTCAGCCGTAGCTAAGCCGCGAACAAAAATACGCGCCAGGTGAATTTCCCTCTGGCGCGTAGAGTACGGGACTGGACA‑TCAATATGCTTAAAGTAAATAAGACTATTCCTGACTATTATTGATAAATGCTTTTAAACCCGCCCGTTAATTAACTCACCAGCTGAAATTCACAATAATTAAGTGATATCGACAGCGCGTTTTTGCATTATTTTGTTACATGCGGCGATGAATTGCCGATTTAACAAACACTTTTCTTTGCTTTTGCGCAAACCCGCTGGCATCAAGCGCCACACAGACGTAACAAGGACTGTTAACCGGGGAAGATATGTCCT  >  NC_000913/88863‑89349                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                       tCGACTTCTTACAGTGTCCGGTGCTGGCAGTGGCTCAACGTCACCGCGATGTCGCAGAGCGTGTGCTGGAGATTGTCCTGGCAAGCCTGGACGAACCGCGTAAGCCAAAACCTG‑GTTTAACGCGCATTAAACGTAATCTCTATCGCCGCGGCGTGCTCAGCCGTAGCTAAGCCGCGAACAAAAATACGCGCCAGGTGAATTTCCCTCTGGCGCGTAGAGTACGGGAATGG‑CACTCAATATCCTTAAAGTa                                                                                                                                                                                                                                               >  2:974290/1‑250 (MQ=255)  cGACTTCTTACAGTGTCCGGTGCTGGCAGTGGCTCAACGTCACCGCGATGTCGCAGAGCGTGTGCTGGAGATTGTCCTGGCAAGCCTGGACGAACCGCGTAAGCCAAAACCTG‑GTTTAACGCGCATTAAACGTAATCTCTATCGCCGCGGCGTGCTCAGCCGTAGCTAAGCCGCGAACAAAAATACGCGCCAGGTGAATTTCCCTCTGGCGCGTAGAGTACGGGAATGGACA‑TCAATATCCTTAAAGTaaa                                                                                                                                                                                                                                             <  1:915117/251‑1 (MQ=255)             aGTGGCCGGTGCTGGCAGTGGCTCAACGTCACCGCGATGTCGCAGAGCGTGTGCTGGAGATTGTCCTGGCAAGCCTGGACGAACCGCGTAAGCCAAAACCTG‑GTTTAACGCGCATTAAACGTAATCTCTATCGCCGCGGCGTGCTCAGCCGTAGCTAAGCCGCGAACAAAAATACGCGCCAGGTGAATTTCCCTCTGGCGCGTAGAGTACGGGAATGGACA‑TCAATATCCTTAAAGTAAATAAGACTATTc                                                                                                                                                                                                                                  <  1:28109/251‑1 (MQ=255)               tgtCCGGTGCTGGCCGTGGCTCAACGTCACCGCGATGTCGCAGAGCGTGTGCTGGAGATTGTCCTGGCAAGCCTGGACGAACCGCGTAAGCCAAAACCTG‑GTTTAACGCGCATTAAACGTAATCTCTATCGCCGCGGCGTGCTCAGCCGTAGCTAAGCCGCGAACAAAAATACGCGCCAGGTGAATTTCCCTCTGGCGCGTAGAGTACGGGAATGGACA‑TCAATATCCTTAAAGTAAATAAGACTATTCCt                                                                                                                                                                                                                                >  1:224965/1‑251 (MQ=255)                gtCCGGTGCTGGCAGTGGCTCAACGTCACCGCGGTGTCGAAGAGCGTGTGCTGGAGATTGTCCTGGCAAGCCTGGCCGAACCGCGTATGCCAAAACCTG‑GGTTGACGCGCATTACACGTAATCTCTATCGCCGCGCCGTGCTCAGCCGTAGCTAAGCCGCGACCAAAAATAAGCGCCCGGTGAATTTCACTCTGGCGCGTAGAGTCCGGGAATGGACA‑TAAATCTCCTTAACGTAAATCCGACTATTCCTg                                                                                                                                                                                                                               >  2:583100/1‑251 (MQ=255)                     gTGCTGGCAGTGGATCAACGTCAACGCGATGTCGCAGAGCGTGTGCTGGAGATTGTCCTGGCAAGCCTGGACGAACCGCGTAAGCCAAAACCTG‑GTTTAACGCGCATTAAACGTAATCTCTATAGCCGCGGCGTGCTCAGCCGTAGCTCAGCCGCGAACAAAAATCAGCGCCAGGTGAATTTCCCTCTGGCGCGTAGAGTACGGGAATGGACA‑TCAATATCCTTAAAGTAAATAAGACTATTCCTgccact                                                                                                                                                                                                                          >  2:993345/1‑246 (MQ=255)                      ggCTGGCAGTGGCTCAACGGCACCGCGAGGTCGCAGAGCGTGTGCTGGAGATTGTCCTGGCAAGCCTGGACGAACCGCGTAAGCCAAAACCTG‑GTTTAACGCGCATTAAACGTAATCTCTATCGCCGCGGCGTGCTCAGCCGTAGCTAAGCCGCGAACAAAAATACGCGCCAGGTGAATTTCCCTCTGGCGCGTAGAGTACGGGAATGGACA‑TCAATATCCTTAAAGTAAATAAGACTATTCCTGACtatt                                                                                                                                                                                                                         <  2:9543/250‑1 (MQ=255)                       gCTGGCAGTGGCTCAACGTCACCGCGATGTCGCAGAGCGTGTGCTGGAGATTGTCCTGGCAAGCCTGGACGAACCGCGTAAGCCAAAACCTG‑GTTTAACGCGCATTAAACGTAATCTCTATCGCCGCGGCGTGCTCAGCCGTAGCTAAGCCGCGAACAAAAATACGCGCCAGGTGAATTTCCCTCTGGCGCGTAGAGTACGGGAATGGACA‑TCAATATCCTTAAAGTAAATAAGACTATTCCTGACtatta                                                                                                                                                                                                                        <  1:868274/251‑1 (MQ=255)                       gCTGGCAGTGGCTCAACGTCACCGCGATGTCGCAGAGCGTGTGCTGGAGATTGTCCTGGCAAGCCTGGACGAACCGCGTAAGCCAAAACCTG‑GTTTAACGCGCATTAAACGTAATCTCTATCGCCGCGGCGTGCTCAGCCGTAGCTAAGCCGCGAACAAAAATACGCGCCAGGTGAATTTCCCTCTGGCGCGTAGAGTACGGGAATGGACA‑TCAATATCCTTAAAGTAAATAAGACTATTCCTGACtatt                                                                                                                                                                                                                         <  1:774629/250‑1 (MQ=255)                         tGGCAGTGGCTCAACGTCACCGCGATGTCGCAGAGCGTGTGCTGGAGATTGTCCTGGCAAGCCTGGACGAACCGCGTAAGCCAAAACCTG‑GTTTAACGCGCATTAAACGTAATCTCTATCGCCGCGGCGTGCTCAGCCGTAGCTAAGCCGCGAACAAAAATACGCGCCAGGTGAATTTCCCTCTGGCGCGTAGAGTACGGGAATGGACA‑TCAATATCCTTAAAGTAAATAAGACTATTCCTGACTattatt                                                                                                                                                                                                                      >  1:135887/1‑251 (MQ=255)                         tGGCAGTGGCTCAACGTCACCGCGATGTCGCAGAGCGTGTGCTGGAGATTGTCCTGGCAAGCCTGGACGAACCGCGTAAGCCAAAACCTG‑GTTTAACGCGCATTAAACGTAATCTCTATCGCCGCGGCGTGCTCAGCCGTAGCTAAGCCGCGAACAAAAATACGCGCCAGGTGAATTTCCCTCTGGCGCGTAGAGTACGGGAATGGACA‑TCAATATCCTTAAAGTAAATAAGACTATTCCTGACTattatt                                                                                                                                                                                                                      >  1:654191/1‑251 (MQ=255)                          ggCAGTGGCTCAACGTCACCGCGATGTCGCAGAGCGTGTGCTGGAGATTGTCCTGGCAAGCCTGGACGAACCGCGTAAGCCAAAACCTG‑GTTTAACGCGCATTAAACGTAATCTCTATCGCCGCGGCGTGCTCAGCCGTAGCTAAGCCGCGAACAAAAATACGCGCCAGGTGAATTTCCCTCTGGCGCGTAGAGTACGGGAATGGACA‑TCAATATCCTTAAAGTAAATAAGACTATTCCTGACTattatt                                                                                                                                                                                                                      <  1:878975/250‑1 (MQ=255)                              gTGGCTCAACGTCACCGCGATGTCGCAGAGCGTGTGCTGGAGATTGTCCTGGCAAGCCTGGACGAACCGCGTAAGCCAAAACCTG‑GTTTAACGCGCATTAAACGTAATCTCTATCGCCGCGGCGTGCTCAGCCGTAGCTAAGCCGCGAACAAAAATACGCGCCAGGTGAATTTCCCTCTGGCGCGTAGAGTACGGGAATGGACA‑TCAATATCCTTAAAGTAAATAAGACTATTCCTGACTATTATTGATaa                                                                                                                                                                                                                 <  1:133663/251‑1 (MQ=255)                                    cAACGTCCCCGCGATGTCTCAGATCGTGTGCTGGAGATTGTTCTGGCAAGCCTGGACGAACCGCGTAAGCCAAAACCTG‑GTTTAACGCGCATTAAACGTAATCTCTATCGCCGCGGCGTGCTCAGCCGTAGCTAAGCCGCGAACAAAAATACGCTCCAGGTGAATTTCCCTCTGGCGCGTAGAGTACGGGAATGGACA‑TCAATATCCTTAAAGTAAATAAGACTATTCCTGACTATTATTGATaagttctt                                                                                                                                                                                                           <  1:318562/251‑7 (MQ=255)                                          cACCGCGATGTCGCAGAGCGTGTGCTGGAGATTGTCCTGGCAAGCCTGGACGAACCGCGTAAGCCAAAACCTG‑GTTTAACGCGCATTAAACGTAATCTCTATCGCCGCGGCGTGCTCAGCCGTAGCTAAGCCGCGAACAAAAATACGCGCCAGGTGAATTTCCCTCTGGCGCGTAGAGTACGGGAATGGACA‑TCAATATCCTTAAAGTAAAAAAGACTATTCCTGACTATTATTGATAAGTTCTTTTAAAc                                                                                                                                                                                                     >  2:758860/1‑251 (MQ=255)                                          cACCGCGATGTCGCAGAGCGAGTGCTGGAGATTGTCCTGGCAAGCCTTGACGAACCGCGTAAGCCAAAACCTG‑GTTTAACGCGCATTAAACGTAATCTCTATCGCCGCGGCGTGCTCAGCCGTAGCTAAGCCGCGAACAAAAATACGCGCCAGGTGAATTTCCCTCTGGCGCGTAGAGTACGGGAATGGACA‑TCAATATCCTTAAAGTAAATAAGACTATTCCTGACTATTATTGATAAGTTCTTTTaaa                                                                                                                                                                                                      <  1:790076/250‑1 (MQ=255)                                                     cGCAGAGCGTGTGCTGGAGATTGTCCTGGCAAGCCTGGACGAACCGCGTAAGCCAAAACCTG‑GTTTAACGCGCATTAAACGTAATCTCTATCGCCGCGGCGTGCTCAGCCGTAGCTAAGCCGCGAACAAAAATACGCGCCAGGTGAATTTCCCTCTGGCGCGTAGAGTACGGGAATGGACA‑TCAATATCCTTAAAGTAAATAAGACTATTCCTGACTATTATTGATAAGTTCTTTTAAACCCGCCCGtta                                                                                                                                                                                           <  1:717748/250‑1 (MQ=255)                                                         gagctggtgCAGGAGAGTGTCATGGCAATCCTGGACGAACCTCGTAAGCCAAAACTTGTGTTTG‑CGCGCATTAAACGTAATCTCTATCGCCGTGACGGGCTCATCCGTAGCTAAGCCGCGAACAAAAATACGCGCCAGGTGAATTTCCTTCTGGCGCGTATAGTACGGTAATGGACA‑TCAATATCCTTAAAGTAAATAATGCTAGACTTGACTATTGTTGATAAGTTCTTTTAACcccgcactggaattaa                                                                                                                                                                                      <  2:595927/245‑12 (MQ=255)                                                            cGTGTGCTGGAGATTGTCCTGGCAAGCCTGGACGAACCGCGTAAGCCAAAACCTG‑GTTTAACGCGCATTAAACGTAATCTCTATCGCCGCGGCGTGCTCAGCCGTAGCTAAGCCGCGAACAAAAATACGCGCCAGGTGAATTTCCCTCTGGCGCGTAGAGTACGGGAATGGACA‑TCAATATCCTTAAAGTAAATAAGACTATTCCTGACTATTATTGATAAGTTCTTTTAAACCCGCCCGTTAATTAACTc                                                                                                                                                                                   <  1:718133/251‑1 (MQ=255)                                                            cGTGGGCTGGAGTTTGTCATGGCAAGACTGGACGAACCGCGTAAGCCAAAACCTG‑GTTTAACGCGCATTAAACGTAATCTCTATCGCCGCGGCGTGCTCAGCCGTAGCTAAGCCGCGAACAAAAATACGCGCCAGGTGAATTTCCCTCTGGCGCGTAGAGTACGGGAATGGACA‑TCAATATCCTTAAAGTAAATAAGACTATTCCTGACTATTATTGATAAGTTCTTTTAAACCCGCCCGTTAATTAACTc                                                                                                                                                                                   <  2:2343/251‑1 (MQ=255)                                                                    ggAGATTGTCCTGGCAAGCCTGGACGAACCGCGTAAGCCAAAACCTG‑GTTTAACGCGCATTAAACGTAATCTCTATCGCCGCGGCGTGCTCAGCCGTAGCTAAGCCGCGAACAAAAATACGCGCCAGGTGAATTTCCCTCTGGCGCGTAGAGTACGGGAATGGACA‑TCAATAGCCTTAAAGTAAATAAGACTATTCCTGACTATTATTGATAAGTTCTTTTAAACCCGCCCGTTAATTAACTCACCAGCTg                                                                                                                                                                           <  1:628740/251‑1 (MQ=255)                                                                     gagaTTGTCCTGGCAAGCCTGGACGAACCGCGTAAGCCACAACCTG‑ATTTAACGCGCATTAAACGTAATCTCTATCGCCGCGGCGTGCTCAGCCGTAGCTAAGCCGCGAACAAAACTACGCGCAAGGTGAATTTCCATCTGGCGCGTAGAGTACAGGAATGGACA‑TCAATATCCTTAAAGTAAATAAGACTATTCCTGACTATTATTGATAAGTTCTTTTAAACCCGCCCGTTAATTAACTCACCAGCTg                                                                                                                                                                           >  2:390998/1‑250 (MQ=255)                                                                      agaTTGTCCTGGCAAGCCTGGACGAACCGCGTAAGCCAAAACCTG‑GTTTAACGCGCAGTAAACGTAATCTCTATCGCCGCGGCGTGCTCAGCCGTAGCTAAGCCGCGAACAAAAATACGCGCCAGGTGAATTTCCCTCTGGCGCGTAGAGTACGGGAATGGACA‑TCAATATCCTTAAAGTAAATAAGACTATTCCTGACTATTATTGATAAGTTCTTTTAAACCCGCCCGTTAATTAACTCACCAGCTGaa                                                                                                                                                                         <  2:409812/251‑1 (MQ=255)                                                                      agaTTGTCCTGGAAAGCCTGGTCGAACCGCGTAAGCAAAAACCTG‑TTTTAACGCGCATTAAACGTAATCTCTAGCGCCGCGGCGTGCTCAGCCGTAGCTAAGCAGCGAACAAAAATACGCGCCAGGTGAATTTCCCTCTGGCGCGTAGAGTCCGGGAATGGACA‑TCAATATCCTTAAAGTAAATAAGACTATTCCTGACTATTATTAATAAGTTATTTTAAACCCGCCCGTTAATTAACTCACCAGCTGaa                                                                                                                                                                         <  2:584646/251‑1 (MQ=255)                                                                          tGTCCTGGCAAGCCTGGACGAACCGCGTAAGCCAAAACCTG‑GTTTAACGCGCATTAAACGTAATCTCTATCGCCGCGGCGTGCTCAGCCGTAGCTAAGCCGCGAACAAAAATACGCGCCAGGTGAATTTCCCTCTGGCGCGTAGAGTACGGGAATGGACA‑TCAATATCCTTAAAGTAAATAAGACTATTCCTGACTATTATTGATAAGTTCTTTTAAACCCGCCCGTTAATTAACTCACCAGCTGAAAtt                                                                                                                                                                      <  1:739778/250‑1 (MQ=255)                                                                           gTCCTGGCAAGCCTGGACGAACCGCGTAAGCCAAAACCTG‑GTTTAACGCGCATTAAACGTAATCTCTATCGCCGCGGCGTGCTCAGCCGTAGCTAAGCCGCGAACAAAAATACGCGCCAGGTGAATTTCCCTCTGGCGCGTAGAGTACGGGAATGGACA‑TCAATATCCTTAAAGTAAATAAGACTATTCCTGACTATTATTGATAAGTTCTTTTAAACCCGCCCGTTAATTAACTCACCAGCTGAAATTc                                                                                                                                                                     <  1:893815/250‑1 (MQ=255)                                                                             ccTGGCAAGCCTGGACGAACCGCGTAAGCCAAAACCTG‑GTTTAACGCGCATTAAACGTAATCTCTATCGCCGCGGCGTGCTCAGCCGTAGCTAAGCCGCGAACAAAAATACGCGCCAGGTGAATTTCCCTCTGGCGCGTAGAGTACGGGAATGGACA‑TCAATATCCTTAAAGTAAATAAGACTATTCCTGACTATTATTGATAAGTTCTTTTAAACCCGCCCGTTAATTAACTCACCAGCTGAAATTcac                                                                                                                                                                   <  1:385175/250‑1 (MQ=255)                                                                                gccAAGCCTGGACGAACCGCGTAAGCCAAATCCTG‑GTGTAACGCGCAATAAACGTAATCTCTACCGCCGCGGCGTGCTAGGCCGTAGCTAAGACGCGAACAAAAATACGCGCCAGGGGAATTTCCCTCTGGCGCGTAGAGTACGGGAATGGACA‑TCAATATCCTTAAAGTAAATAAGACTATTCCTGACAATTATTGATAAGTTCTTTTAAACCCGCCCGTTAATTAACTCACCAGCTTAAATTCACaa                                                                                                                                                                 <  2:768161/247‑1 (MQ=255)                                                                                  cAAGCCTGGACGAACCGCGTAAGCCAAAACCTG‑GTTTAACGCGCATTAAACGTAATCTCTATCGCCGCGGCGTGCTCAGCCGTAGCTAAGCCGCGAACAAAAATACGCGCCAGGTGAATTTCCCTCTGGCGCGTAGAGTACGGGAATGGACA‑TCAATATCCTTACAGTAAATAAGACTATTCCTGACTATTATTGATAAGTTCTTTTAAACCCGCCCGTTAATTAACTCACCAGCTGAAATTCACaataat                                                                                                                                                             >  2:587110/1‑251 (MQ=255)                                                                                    aGCCTGGACGAACCGCGTAAGCCAAAACCTG‑GTTTAACGCGCATTAAACGTAATCTCTAACGCCGCGGCGTGCGCAGCCGTAGCTAAGCCGCGAACAAAAATACGCGCCAGGTGAATTTCCCTCTGGCGCGTAGAGTACGGGAATGGACA‑TCAATATCCTTAAAGGAAATAAGACTATTCCTGACTATTATTGATAAGTTCTTTTAAACCCGCCCGTTAATTAACACACCAGCTGAAATTCACAATAATTa                                                                                                                                                           <  1:793413/251‑1 (MQ=255)                                                                                    aGCCTGGAACAACCGCGTAAGTCAAAACCTG‑GTTTAACGCGCATTAAACGTAATCTCTATCGCCGCGGCGTGCTCAGCCGTAGCTAAGCCGCGAACAAAAATACGCGCCAGGTGAATTTCCCTCTGGCGCGTAGAGTACGGGAATGGACA‑TCAATATCCTTAAAGTAAATAAGACTATTCCTGACTATTATTGATAAGTTCTTTTAAACCCGCCCGTTAATTAACTCACCAGCTGAAATTCACAATAATTa                                                                                                                                                           <  1:525411/251‑1 (MQ=255)                                                                                      ccTGGACGAACCGCGTAAGCCAAAACCTG‑GTTTAACGCGCATTAAACGTAATCTCTATCGCCGCGGCGTGCTCAGCCGTAGCTAAGCCGCGAACAAAAATACGCGCCAGGTGAATTTCCCTCTGGCGCGTAGAGTACGGGAATGGACA‑TCAATATCCTTAAAGTAAATAAGACTATTCCTGACTATTATTGATAAGTTCTTTTAACACCGCCCGTTAATTAACTCACCAACTGAAATTCACAATAATTa                                                                                                                                                           >  2:805962/1‑249 (MQ=255)                                                                                            cGAACCGCGTGAGCCAAAACCTG‑GTTTAACGCGCATTAAACGAAATCTATATCGCCGCGGCGTGCTCAGCCGTAGCTAAGCCGCGAACAAAAATACGCGCCAGGTGAATTTCACTCTGGCGCGTAGAGGACGGGAATGGACA‑TACATATCCTTAAAGTAAATAAGACTATTCCTGACTATTATTGCTAAGTTCTTTTAAACCCGCCCGTTATTTAACTCACCAGCTGAAATTCACAATAATTAAGTGatat                                                                                                                                                   >  2:612447/1‑251 (MQ=255)                                                                                                ccGCGTAAGCCAAAACCTG‑GTTTAACGCGCATTAAACGTAATCTCTATCGCCGCGGCGTGCTCAGCCGTAGCTAAGCCGCGAACAAAAATACGCGCCAGGTGAATTTCCCTCTGGCGCGTAGAGTACGGGAATGGACA‑TCAATATCCTTAAAGTAAATAAGACTATTCCTGACTATTATTGATAAGTTCTTTTAAACCCGCCCGTTAATTAACTCACCAGCTGAAATTCACAATAATTAAGTGATATCGAc                                                                                                                                               <  1:382104/251‑1 (MQ=255)                                                                                                ccGCGTAAGCCAAAACCTG‑GTTTAACGCGCATTAAACGTAATCTCTATCGCCGCGGCGTGCTCAGCCGTAGCTAAGCCGCGAACAAAAATACGCGCCAGGTGAATTTCCCTCTGGCGCGTAGAGTACGGGAATGGACA‑TCAATATCCTTAAAGTAAATAAGACTATTCCTGACTATTATTGATAAGTTCTTTTAAACCCGCCCGTTAATTAACTCACCAGCTGAAATTCACAATAATTAAGTGATATCGAc                                                                                                                                               <  1:120147/251‑1 (MQ=255)                                                                                                        gCCAAAACCTG‑GTTTAACGCGCATTAAACGTAATCTCTATCGCCGCGGCGTGCTCAGCCGTAGCTAAGCCGCGAACAAAAATACGCGCCAGGTGAATTTCCCTCTGGCGCGTAGAGTACGGGAATGGACA‑TCAATATCCTTAAAGTAAATAAGACTATTCCTGACTATTATTGATAAGTTCTTTTAAACCCGCCCGTTAATTAACTCACCAGCTGAAATTCACAATAATTAAGTGAGATCGACAGCGCGtt                                                                                                                                       <  2:629567/251‑1 (MQ=255)                                                                                                            aaaCCTG‑GTTTAACGCGCATTAAACGTAATCTCTATCGCCGCGGCGTGCTCAGCCGTAGCTAAGCCGCGAACAAAAATACGCGACAGGTGAATTTTCCTCTGGCGCGTAGAGTACGGGAATGGACA‑TCAATATCCTTAAAGTAAATAAGACTATTCCTGACTATTATTGATAAGTTCTTTTAAACCCGCCCGTTAATTAACTCACCAGCTGAAATTCACAATAATTAAGTGATATCGACAGCGCGTTTTTg                                                                                                                                   <  1:382646/251‑1 (MQ=255)                                                                                                                         aCGCGCATTAAACGTAATCTCTATCGCCGCGGCGTGCTCAGCCGTAGCTAAGCCGCGAACAAAAATACGCGCCAGGTGAATTTCCCTCTGGCGCGTAGAGTACGGGAATGGACA‑TCAATATCCTTAAAGTAAATAAGACTATTCCTGACTATTATTGATAAGTTCTTTTAAACCCGCCCGTTAATTAACTCACCAGCTGAAATTCACAATAATTAAGTGATATCGACAGCGCGTTTTTGCATTATTTTGtt                                                                                                                       <  1:811498/251‑1 (MQ=255)                                                                                                                            cgcATTAAACGTAATCTCTATCGCCGCGGCGTGCTCAGCCGTAGCTAAGCCGCGAACAAAAATACGCGCCAGGCGAATTTCCCTCTGGCGCGTAGAGTACGGGAATGGACA‑TCAATATCCTTAAAGTAAATAAGACTATTCCTGAATATTATTGATAAGTTCTTTTAAACCCGCCCGTTAATTAACTCACCAGCTGAAATTCACAATAATTAAGTGCTATCGACAGCGCGTTTTTGCATTATTTTGTTAc                                                                                                                     >  1:557415/1‑250 (MQ=255)                                                                                                                                  aaaCGTAATCTCTATCGCCGCGGCGTGCTCAGCCGTAGCTAAGCCGCGAACAAAAATACGCGCCAGGTGAATTTCCCTCTGGCGCGTAGAGTACGGGAATGGACA‑TCAATATCCTTAAAGTAAATAAGACTATTCCTGACTATTATTGATAAGTTCTTTTAAACCCGCCCGTTAATTAACTCACCAGCTGAAATTCACAATAATTAAGTGATATCGACAGCGCGTTTTTGCATTATTTTGTTACATgc                                                                                                                 >  2:458376/1‑248 (MQ=255)                                                                                                                                  aaaCGTAATCTCTATCGCCGCGGCGTGCTCAGCCGTAGCTAAGCCGCGAACAAAAATACGCGCCAGGTGAATTTCCCTCTGGCGCGTAGAGTACGGGAATGGACA‑TCAATATCCTTAAAGTAAATAAGACTATTCCTGACTATTATTGATAAGTTCTTTTAAACCCGCCCGTTAATTAACTCACCAGCTGAAATTCACAATAATTAAGTGATATCGAAAGCGCGTTTTTGCATTATTTTGTTACATgc                                                                                                                 >  1:42745/1‑248 (MQ=255)                                                                                                                                        aaTCTCTCTCGCCGCGGCGTGCTCAGCCGTAGCTAAGCCGCGAACAAAAATACGCGCCAGGTGAATTTCCCTCTGGCGCGTAGAGTACGGGAATGGACA‑TCAATATCCTTAAAGTAAATAAGACTATTCCTGACTATTATTGATAAGTTCTTTTAAACCCGCCCGTTAATTAACTCACCAGCTGAAATTCACAATAATTAAGTGATATCGACAGCGCGTTTTTGCATTATTTTGTTACATGCAGAGATGa                                                                                                         <  2:145928/250‑1 (MQ=255)                                                                                                                                             ctATCGCCGCGGCGTGCTCAGCCGTAGCTAAGCCGCGAACAAAAATACGCGCCAGGTGAATTTCCCTCTGGCGCGTAGAGTACGGGAATGGACA‑TCAATATCCTTAAAATAAATAAGACTATTCCTGACTATTATTGATAAGTTCTTTTAAACCCGCCCGTTAATTAACTCACCAGCTGAAATTCACAATAATTAAGTGATATCGACAGCGCGTTTTTGCATTATTTTGTTAAATGCAGAGATGAATTGcc                                                                                                   >  2:753490/1‑251 (MQ=255)                                                                                                                                                acgccgcGGCGTGCTCAGCCGTAGCTAAGCCGCGAACAAAAAGACGCGCCAGGTGAATTTCCCTCTGGCGCGTAGAGTACGGGAATGGACA‑TCAATATCCTTAAAGTAAATAAGACTATTCCTGACTATTATTGATAAGTTCTTTTAAACCCGCCCGTTAATTAACTCACCAGCTGAAATTCACAATAATTAAGTGATATCGACAGCGCGTTTTTGCATTATTTGGTTACATGCAGAGATGAATTGCCGAt                                                                                                <  2:138653/250‑1 (MQ=255)                                                                                                                                                 cgccgcGGCGTGCTCAGCCGTAGCTAAGCCGCGAACAAAAATACGCGCCAGGTGAATTTCCCTCTGGCGCGTAGAGTACGGGAATGGACA‑TCAATATCCTTAAAGTAAATAAGACTATTCCTGACTATTATTGATAAGTTCTTTTAAACCCGCCCGTTAATTAACTCACCAGCTGAAATTCACAATAATTAAGTGATATCGACAGCGCGTTTTTGCATTATTTTGTTACATGCAGAGATGAATTGCCGAt                                                                                                <  1:291423/250‑1 (MQ=255)                                                                                                                                                 cgccgcGGCGTGCTCAGCCGTAGCTAAGCCGCGAACAAAAATACGCGCCAGGTGAATTTCCCTCTGGCGCGTAGAGTACGGGAATGGACA‑TCAATATCCTTAAAGTAAATAAGACTATTCCTGACTATTATTGATAAGTTCTTTTAAACCCGCCCGTTAATTAACTCACCAGCTGAAATTCACAATAATTAAGTGATATCGACAGCGCGTTTTTGCATTATTTTGTTACATGCAGAGATGAATTGCCGAt                                                                                                <  1:987486/250‑1 (MQ=255)                                                                                                                                                  gccgcGGCGTGCTCAGCCGTAGCTAAGCCGCGAACAAAAATACGCGCCAGGTGAATTTCCCTCTGGCGCGTAGAGTACGGGAATGGACA‑TCAATATCCTTAAAGTAAATAAGACTATTCCTGCCTATTATTGCTAAGTTCTTTTAAACCCGCCCGTTAATTAACTCACCAGCTGAAATTCACAATAATTAAGTGATATCGACAGCGCGTTTTTGCATTATTTTGTTACATGCAGAGATGAATTGCCGAttt                                                                                              >  2:12922/1‑251 (MQ=255)                                                                                                                                                   ccgcGGCGTGCTCAGCCGTAGCTAAGCCGCGAACAAAAATACGCGCCAGGTGAATTTCCCTCTGGCGCGTAGAGTACGGGAATGGACA‑TCAATATCCTTAAAGTAAATAAGACTATTCCTGACTATTATTGAGAAGTTCTTTTAAACCCGCCCGTTAATTAACTCACCAGCTGAAATTCACAATAATTAAGTGATATCGACAGCGCGTTTTTGCATTATTTTGTTACATGCAGAGATGAATTGCCGATTTa                                                                                             <  1:606104/251‑1 (MQ=255)                                                                                                                                                     gcggcgTGCTCAGCCGTAGCTAAGCCGCGAACAAAAATACGCGCCAGGTGAATTTCCCTCTGGCGCGTAGAGTACGGGAATGGACA‑TCAATATCCTTAAAGTAAATAAGACTATTCCTGACTATTATTGATAAGTTCTTTTAAACCCGCCCGTTAATTAACTCACCAGCTGAAATTCACAATAATTAAGTGATATCGACAGCGCGTTTTTGCATTATTTTGTTACATGCAGAGATGAATTGCCGATTTaac                                                                                           >  1:229572/1‑251 (MQ=255)                                                                                                                                                      cggcgTGCTCAGCCGTAGCTAAGCCGCGAACAAAAATACGCGCCAGGTGAATTTCCCTCTGGCGCGTAGAGTACGGGAATGGACA‑TCAATATCCTTAAAGTAAATAAGACTATTCCTGACTATTATTGATAAGTTCTTTTAAACCCGCCCGTTAATTAACTCACCAGCTGAAATTCACAATAATTAAGTGATATCGACAGCGCGTTTTTGCATTATTGTGTTACATGCAGAGATGAATTGCCGATTTaaca                                                                                          <  1:285426/251‑1 (MQ=255)                                                                                                                                                        gcgTGCTCAGCCGTAGCTAAGCCGCGAACAAAAATACGCGCCAGGTGAATTTCCCTCTGGCGCGTAGAGTACGGGAATGGACA‑TCAATATCCTTAAAGTAAATAAGACTATTCCTGACTATTATTGATAAGTTCTTTTAAACCCGCCCGTTAATTAACTCACCAGCTGAAATTCACAATAATTAAGTGATATCGACAGCGCGTTTTTGCATTATTTTGTTACATGCAGAGATGAATTGCCGATTTaacaag                                                                                        <  1:458135/251‑2 (MQ=255)                                                                                                                                                             cTCAGCCGTAGCTAAGCCGCGAACAAAAATACGCGCCCGGTGAATTTCCCTCTGGCGCGTAGAGTACGGGAATGGACA‑TCAATATCCTTAAAGTCAATAAGACTATTACTGACTATTCTTGATAAGTTCTTTTAAACCCGCCCGTTAATTAACTCACCAGCTGAACTTCACAATAATTAAGTGATATCGACAGCGCGTTTTTGCATTATTTTGTTACATGAAGAGATGAATTGCCGATTTaacacgaactt                                                                                   >  2:738021/1‑244 (MQ=255)                                                                                                                                                               cAGCCGTAGCTAAGCCGCGAACAAAAATACGCGCCAGGTGAATTTCCCTCTGGCGCGTAGAGTACGGGAATGGACA‑TCAATATCCTTAAAGTAAATAAGACTATTCCTGACTATTATTGATAAGTTCTTTTAAACCCGCCCGTTAATTAACTCACCAGCTGAAATTCACAATAATTAAGTGATATCGACAGCGCGTTTTTGCATTATTTTGTTACATGCAGAGATGAATTGCCGATTTAACAAGCACtttt                                                                                 <  1:607162/251‑1 (MQ=255)                                                                                                                                                                 gCCGTAGCTAAGCCGCGAACAAAAATACGCGCCAGGTGAATTTCCCTCTGGCGCGTAGAGTACGGGAATGGACA‑TCAATATCCTTAAAGTAAATAAGACTATTCCTGACTATTATTGATAAGTTCTTTTAAACCCGCCCGTTAATTAACTCACCAGCTGAAATTCACAATAATTAAGGGATATCGACAGCGCGTTTTTGCATTATTTTGTTACATGCAGAGATGAATTGCCGATTTAACAAGCACTTTTCt                                                                               <  2:452821/251‑1 (MQ=255)                                                                                                                                                                   cGTAGCTAAGCCGCGAACAAAAATACGCGCCAGGTGAATTTCCCTCTGGCGCGTAGAGTACGGGAATGGACA‑TCAATATCCTTAAAGTAAATAAGACTATTCCTGACTATTATTGATAAGTTCTTTTAAACCCGCCCGTTAATTAACTCACCAGCTGAAATTCACAATAATTAAGTGATATCGACAGCGCGTTTTTGCATTATTTTGTTACATGCAGAGATGAATTGCCGATTTAACAAGCACTTTTCttt                                                                             <  1:221974/251‑1 (MQ=255)                                                                                                                                                                       acTAAGCTGCGCACAAAAATACACGCCAGTTGCATTTCCCTCTGGCGCGTAGTGTACGGGAATGAACA‑TCACTATCCTTAAAGTAAATAAGACTTTTCCTGACTATTATTGATAAGTTCTTTTAAACCAGCCCGTTAATTAACTCACCAGCTGAAATTCACAATAATTAAGTGATATCGACGGCGCGTTTTTGCATTATTTTGTTACATGCAGAGATGAATTGACGATTAAACAAGAACTTTTCTTTGCtt                                                                         >  2:736325/2‑251 (MQ=255)                                                                                                                                                                          aaGCCGCGAACAAAAATACGCGCCAGGTGAATTTCCCTCTGGCGCGTAGAGTACGGGAATGGACA‑TCAATATCCTTAAAGTAAATAAGACTATTCCTGACTATTATTGATAAGTTCTTTTAAACCCGCCCGTTAATTAACTCACCAGCTGAAATTCACAATAATTAAGTGATATCGACAGCGCGTTTTTGCATTATTTTGTTACATGCAGAGATGAATTGCCGATTTAACAAGCACTTTTCTTTGCtttt                                                                       <  1:756358/250‑1 (MQ=255)                                                                                                                                                                          aaGCCGCGAACAAAAATACGCGCCAGGTGAATTTCCCTCTGGCGCGTAGAGTACGGGAATGGACA‑TCAATATCCTTAAAGTAAATAAGACTATTCCTGACTATTATTGATAAGTTCTTTTAAACCCGCCCGTTAATTAACTCACCAGCTGAAATTCACAATAATTAAGTGATATCGACAGCGCGTTTTTGCATTATTTTGTTACATGCAGAGATGAATTGCCGATTTAACAAGCACTTTTCTTTGCttt                                                                        <  2:135887/249‑1 (MQ=255)                                                                                                                                                                          aaGCCGCGAACAAAAATACGCGCCAGGTGAATTTCCCTCTGGCGCGTAGAGTACGGGAATGGACA‑TCAATATCCGTAAAGTAAATAAGACTATTCCTGACTATTATTGATAAGTTCTTTTAAACCCGCCCGTTAATTAACTCACCAGCTGAAATTCACAATAATTAAGTGATAGCGACAGCGCGTTTTTGCATTATTTTGTTACATGCAGAGATGAATTGCCGATTTAACAAGCACTTTTCTTTGCTTTTg                                                                      <  1:890448/251‑1 (MQ=255)                                                                                                                                                                                        aaTACGCGCCAGGTGAATTTCCCTCTGGCGCGTAGAGTACGGGAATGGACA‑TCAATATCCTTAAAGTAAATAAGACTATTCCTGACTATTATTGATAAGTTCTTTTAAACCCGCCCGTTAATTAACTCACCAGCTGAAATTCACAATAATTAAGTGATATCGACAGCGCGTTTTTGCATTATTTTGTTACATGCAGAGATGAATTGCCGATTTAACAAGCACTTTTCTTTGCTTTTGCGCAAACCCGCTgg                                                        <  1:3046/251‑1 (MQ=255)                                                                                                                                                                                                          ttCCCTCTGGCGCGTAGAGTACGGGAATGGACA‑TCAATATCCTTAAAGTAAATAAGACTATTCCTGACTATTATTGATAAGTTCTTTTAAACCCGCCCGTTAATTAACTCACCAGCTGAAATTCACAATAATTAAGTGATATCTACAGCGCGTTTTTGCATTATTTTGTTACATGCAGAGATGAATTGCCGATTTAACAAGCACTTTTCTTTGCTTTTGCGCATACCCGCTGGCATCAAGCGCCGCACAg                                       <  1:753490/250‑1 (MQ=255)                                                                                                                                                                                                            cccTCTGGCGCGTAGAGTACGGGAATGGACA‑TAAATATCCTTAAAGTAAATAAGACTATTCCTGACTATTATTGATAAGTTCTTTTAAACCCGCCCGTTAATTAACTCACCAGCTGAAATTCACAATAATTAAGTGATATCGACAGCGCGTTTTTGCATTATTTTGTTACATGCAGAGATGAATTGCCGATTTAACAAGCACTTTTCTTTGCTTTTGCGCAAAGCCGCTGGCATCAAGCGCCACACAGACg                                    >  2:680917/1‑251 (MQ=255)                                                                                                                                                                                                               tctGGCGCGTAGAGTACGGGAATGGAAA‑TCAATATCCTTAAAGTAAATAAGACTATTCCTGACTATTATTGATAAGTTCTTTTAAACCCGCCCGTTAATTAACTCACCAGCTGAAATTCACAATAATTAAGTGATATCGACAGCGCGTTTTTGCATTATTTTGTTACATGCAGAGATGAATTGCCGATTTAACAAGCACTTTTCTTTGCTTTTGCGCAAACCCGCTGGCATCAAGCGCCACCCAGACgca                                  >  2:905929/1‑248 (MQ=255)                                                                                                                                                                                                                          gagTACGGGAATGGACA‑TCAATATCCTTAAAGTAAATAAGACTATTCCTGACTATTATTGATAAGTTCTTTTAAACCCGCCCGTTAATTAACTCACCAGCTGAAATTCACAATAATTAAGTGATATCGACAGCGCGTTTTTGCATTATTTTGTTACATGCAGAGATGAATTGCCGATTTAACAAGCACTTTTCTTTGCTTTTGCGCAAACCCGCTGGCATCAAGCGCCACACAGACGCAACAGGGACTGtt                      <  1:986919/251‑1 (MQ=255)                                                                                                                                                                                                                                     tGGACA‑TCAATATCCTTAAAGTAAATAAGACTATTCCTGACTATTATTGATAAGTTCTTTTAAACCCGCCCGTTAATTAACTCACCAGCTGAAATTCACAATAATTAAGTGATATCGACAGCGCGTTTTTGCATTATTTTGTTACATGCAGAGATGAATTGCCGATTTAACAAGCACTTTTCTTTGCTTTTGCGCAAACCCGCTGGCATCAAGCGCCACACAGACGCAACAGGGACTGTTAACCGGGGa             >  1:719610/1‑249 (MQ=255)                                                                                                                                                                                                                                          a‑TCAATATCCTTAAAGTAAATAAGACTATTCCTGACTATTATTGATAAGTTCTTTTAAACCCGCCCGTTAATTAACTCACCAGCTGAAATTCACAATAATTAAGTGATCTCGACAGCGCGTTTTTGCATTATTTTGTTACATGCAGAGATGAATTGCCGATTTAACAAGCACTTTTCTTTGCTTTTGCGCAAACCCGCTGGCATCAAGCGCCACACAGAAGCAACAGGGACTGTTAACCGGGGAAGATATg      >  2:317366/1‑251 (MQ=255)                                                                                                                                                                                                                                          a‑TCAATATCCTTAAAGTAAATAAGACTATTCCTGACTATTATTGATAAGTTCTTTTAAACCCGCCCGTTAATTAACTCACCAGCTGAAATTCACAATAATTAAGTGATATCGACAGCGCGTTTTTGCATTATTTTGTTACATGCAGAGATGAATTGCCGATTTAACAAGCACTTTTCTTTGCTTTTGCGCAAACCCGCTGGCATCAAGCGCCACACAGACGCAACAGGGACTGTTAACCGGGGAAGATATg      >  1:441213/1‑251 (MQ=255)                                                                                                                                                                                                                                               atatCCTTAAAGTAAATAAGACTATTCCTGACTATTATTGATAAGTTCTTTTAAACCCGCCCGTTAATTAACTCACCAGCTGAAATTCACAATAATTAAGTGATATCGACAGCGCGTTTTTGCATTATTTTGTTACATGCAGAGATGAATCGCCGATTTAACAAGCACTTTTCTTTGCTTTTGCGCAAACCCGCTGGCATCAAGCGCCACACAGACGCAACAGGGACTGTTAACCGGGGAAGATATGTCCt  >  1:286547/1‑251 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                       TCGACTTCTTACAGTGTCCGGTGCTGGCAGTGGCTCAACGTCACCGCGATGTCGCAGAGCGTGTGCTGGAGATTGTCCTGGCAAGCCTGGACGAACCGCGTAAGCCAAAACCTG‑GTTTAACGCGCATTAAACGTAATCTCTATCGCCGCGGCGTGCTCAGCCGTAGCTAAGCCGCGAACAAAAATACGCGCCAGGTGAATTTCCCTCTGGCGCGTAGAGTACGGGACTGGACA‑TCAATATGCTTAAAGTAAATAAGACTATTCCTGACTATTATTGATAAATGCTTTTAAACCCGCCCGTTAATTAACTCACCAGCTGAAATTCACAATAATTAAGTGATATCGACAGCGCGTTTTTGCATTATTTTGTTACATGCGGCGATGAATTGCCGATTTAACAAACACTTTTCTTTGCTTTTGCGCAAACCCGCTGGCATCAAGCGCCACACAGACGTAACAAGGACTGTTAACCGGGGAAGATATGTCCT  >  NC_000913/88863‑89349

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑