| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 86,315 | C→T | T229M (ACG→ATG) | ilvI → | acetolactate synthase/acetohydroxybutanoate synthase, catalytic subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 86,315 | 0 | C | T | 97.7% | 244.3 / NA | 92 | T229M (ACG→ATG) | ilvI | acetolactate synthase/acetohydroxybutanoate synthase, catalytic subunit |
| Reads supporting (aligned to +/- strand): ref base C (1/1); major base T (36/50); minor base G (0/2); total (39/53) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.11e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01 | |||||||||||
GCTGGCGGCAAGTGGTCGCCCAGGACCAGTAGTCGTTGATTTACCGAAAGATATTCTTAATCCGGCGAACAAATTACCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTACAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTAGCGGCAAAAAAACCGGTTGTCTACGTAGGCGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAACGGTGGAGGCGTTGAATCTGCCCGTT‑GT‑TTGCTCATT‑GATGGGGCTGGGGGCGTTT‑CCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAA‑TGCACGGTACCTACGAAGCCAATATGACGATGCATAACGCGG‑ATGTG‑ATTTTCGCCGTCGGGGTAC‑GATTTGATGACCGAACGACGAACAATCTGGCAA‑AGTACTGC‑CCAAATGCCACTGTTCTGCATATCGATATTGATCCTACTTCCATT > NC_000913/86070‑86559 | gctggcGGCAAGTGGTCGCCCAGGACCAGTAGTCGTTGATTTACCGAAAGATATTCTTAATCCGGCGAACAAATTACCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTACAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAAtggtgg < 1:187936/251‑1 (MQ=255) ggcggcAAGTGGTCGCCCAGGACCAGTAGTCGTTGATTTACCGAAAGATATTCTTAATCCGGCGAACAAATTACCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTACAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAATGGTGGa < 1:351230/249‑1 (MQ=255) ggcAAGTGGTCGCCCAGGACCAGTAGTCGTTGATTTACCGAAAGATATTCTTAATCCGGCGAACAAATTACCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTACAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAATGGTGGAGGCGt < 1:728283/251‑1 (MQ=255) gcAAGTGGTCGCCCAGGACCAGTAGTCGTTGATTTACCGAAAGATATTCTTAATCCGGCGAACAAATTACCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTACAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAATGGTGGAGGCGtt > 1:7570/1‑251 (MQ=255) cAAGTGGTCGCCCAGGACCTGTAGTCGTTGATTTACCGAAAGATATTCTTAATCCGGCGAACAAATTTCCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTACAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAATGGTGGAGGCGTTg < 2:298731/251‑1 (MQ=255) gtgggctcccAGGACCAGTTGTAGTTGATTTACCGAAAGATATTCTTAACCCTGCGAACAAATTACCCTATGTCTGGCCTGAGTCGGTCATTATGCGTTCTTACAATCCCACTCCTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAATACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAATGGTGGAGGCGTTGAAt < 2:521105/244‑1 (MQ=255) gtcgccacgtaCCAGTAGTCGTTGAGTTACCGAAAGAGATTCTTAATCCTGCGAACAAATTCCCCTAGGTCTGGCCGGAGGCGGTCAGTAGTCGTTCTTATAATCCCACTACTACCGGACATAAAGCGCAAATTAAGCGTTCGCTGCAAACGCTTGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATTACGGCGGGCTGTCAGCAGCAGTTGAAAGAAATGGTGGAGGCGtcaaatcgg < 2:84705/241‑9 (MQ=255) cAGGACCAGTAGTCGTTGATTTACCGAAAGATATTCTTAATCCGGCGAACAAATTACCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTACAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAATGGTGGAGGCGTTGAATCTGCCCgtt < 1:406182/251‑1 (MQ=255) cAGGACCAGTAGCAGTTGATGTCCCGAAAGATATTCGTAAACCGGCGAAAAAATTACCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTCCAATACCACTACTAGCGGACATAAAGGGCAAATTAAGTGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCGCCGTAGGTGGTGGGGCAATCACGGTGGGCTGCCATCAGCAGTTGAAAGAAATGGTGGAGGCGTTGAAACTGCTCgtt < 2:286299/251‑1 (MQ=255) ggcccAGTAGTCGTTGATTTACCGAAAGATATTCTTAATCCGGCGAACAAATTACCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTACAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCAACCGCAGTTGAAAGAAATGGAGGAGGCGGTGAATCTGCCCgtt‑gt > 1:838121/4‑251 (MQ=255) ggACCAGTAGTCGTTGATTTACCGAAAGATATTCTTAATCCGGCGAACAAATTACCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTACAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAATGGTGGAGGCGTTGAATCTGCCCgtt‑gt < 1:345412/251‑1 (MQ=255) cAGTAGTCGTTGATTTACCGAAAGATATTCTTAATCCGGCGAACAAATTACCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTACAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAATGGTGGAGGCGTTGAATCTGCCCGTT‑Gt‑ttcc < 2:804038/251‑3 (MQ=255) ttACCGAAAGATATTCTTAATCCGGCGAACAAATTACCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTACAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAATGGTGGAGGCGTTGAATCTGCCCGTT‑GT‑TTCCTCATT‑GATGGGGc < 2:942461/250‑1 (MQ=255) ccGAAAGATATTCTTAATCCGGCGAACAAATTACCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTACAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAATGGTGGAGGCGTTGAATCTGCCCGTT‑GT‑TTCCTCATT‑GATGGGGCTggg < 1:194006/251‑1 (MQ=255) cGAAAGATATTCTTAATCCGGCGAACAAATTACCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTACAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAATGGTGGAGGCGTTGAATCTGCCCGTT‑GT‑TTCCTCATT‑GATGGGGCTgggg < 1:270776/251‑1 (MQ=255) aaGATATTCTTAATCCGGCGAACAAATTACCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTACAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAATGGTGGAGGCGTTGAATCTGCCCGTT‑GT‑TTCCTCATT‑GATGGGGCTGGGGGCg > 1:442968/1‑251 (MQ=255) gATATTCTTAATCCGGCGAACAAATTACCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTACAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAATGGTGGAGGCGTTGAATCTGCCCGTT‑GT‑TTCCTCATT‑GATGGGGCTGGGGGCGtt < 1:883222/251‑1 (MQ=255) tAATCCGGCGAACAAATTACCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTACAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTAGCGGCAAAAAAACCGGTTGTCTACGTAGGCGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTTAACACAACGGTGGGGGCGTTGAATCTTCCCGC‑‑GTGTTGCTCCTT‑GATGGGGATgggggagtgtcccgaa > 1:666827/1‑239 (MQ=255) aaTCCGGCGAACAAATTACCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTACAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAATGGTGGAGGCGTTGAATCTGCCCGTT‑GT‑TTCCTCATT‑GATGGGGCTGGGGGCGTTT‑CCGGCaa < 2:203042/250‑1 (MQ=255) aTCCGGCGAACAAATTACCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTACAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGGGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAATGGTGGGGGCGTTGAATCTGCCCGTT‑GT‑TTCCTCATT‑GATGGGGCTGGGGGCGTTT‑TCGGCAACg > 1:37172/1‑251 (MQ=255) tCCGGCGAACAAATTACCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTACAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAATGGGGGAGGCGTTGAATCTGCCCGTT‑GT‑TTCCTCATT‑TATGGGGCTGGGGGCGTTT‑CCGGCAACGc > 1:387159/1‑251 (MQ=255) cAAATTACCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTACAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAATGGTGGAGGCGTTGAATCTGCCCGTT‑GT‑TTCCTCATT‑GGTGGGGCTGGGGGCGGTT‑CCGGCAACGCATCGTccgg > 1:889023/1‑248 (MQ=255) aTTACCCTATGTCTGGCCGGAGTCGGGCAGTATGCGTTCTTACAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAATGGTGGAGGCGTTGAATCTGCCCGTT‑GT‑TTCCTCATT‑GATGGGGCTGGGGGCGTTT‑CCGGCAACGCATCGTCAGGCAc < 2:192614/251‑1 (MQ=255) ttACCCTATGTCTGGCCGGAGACGGTCAGTATGCGTTCTTACAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGAGGTGGGGCAATCACGGCGGGCTGCCATCAGCCGTTGGAAAAAAAGGTGGAGGCGTTGGATCTTCCCGTT‑GT‑TTTCTCATT‑TCTGGGGCAGGGGGGGGTT‑TCGGCAACGccccttccggcacc > 2:839920/1‑238 (MQ=255) aTGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTACAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAATGGTGGAGGCGTTGAATCTGCCCGTT‑GT‑TTCCTCATT‑GATGGGGCTGGGGGCGTTT‑CCGGCAACGCATCGTCAGGCACTGGGCAt < 1:67772/250‑1 (MQ=255) tGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTACAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTAGCGGCAAAAAAACCGGTTGTCTACGTAGGCGGTGGGGCAATCACGGCGGGCTGCCCTCAGACGTTGGAAGAAAAGGGGGGGGCGTTGGATCTTCCCGTT‑GT‑TTTCTCATTTGTTGGGCTTGGGGGGGTTT‑CGGGCacgactcggtcgggcctgggcgggg > 2:492074/1‑227 (MQ=255) ccGGAGTCGGTCAGTATGCGTTCTTACGATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAATGGTGGAGGCGTTGAATCTGCCCGTT‑GT‑TTCCTCATT‑GATGGGGCTGGGGGCGTTT‑CCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAA‑t < 1:798545/251‑1 (MQ=255) cGGAGTCGGTCAGTATGCGTTCTTACAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAGCGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGAGGTGGGGCAATCACGGCGGGCTGCCATAAGCAGTTGAAAGAAATGGTGGAGGCGTTGAATCTGCCCGTT‑GT‑TTCCTCATT‑GATGGGGCTGGGGGCGTTT‑CCGGCCACGCATCGGCAGGCACTGGGCATGCTGGGAA‑Tg > 1:869133/1‑251 (MQ=255) cGGAGTCGGTCAGTATGCGTTCTTACAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAATGGTGGAGGCGTTGAATCTGCCCGTT‑GT‑TTCCTCATT‑GATGGGGCTGGGGGCGTTT‑CCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAA‑Tg > 1:532536/1‑251 (MQ=255) ggAGTCGGTCAGTATGCGTTCTTACAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAATGGTGGAGGCGTTGAATCTGCCCGGT‑GT‑TTCCTCCTT‑GATGGGGCTGGGGGCGTTTTCGGGCAA‑GCATCGTCCAGCACTGGGCCTGGTggggattg > 2:479495/1‑246 (MQ=255) gTCGGTCAGTATGCGTTCTTACAATCCCACTACTGCCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAATGGTGGAGGCGTTGAATCTGCCCGTT‑GT‑TTCCTCATT‑GATGGGGCTGGGGGCGTTT‑CCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAA‑TGCACg < 2:778671/251‑1 (MQ=255) tCTTACAATCCCACTACGTCCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAATGGTGGAGGCGTTGAATCTGCCCGTT‑GT‑TTCCTCATT‑GATGGGGCTGGGGGCGTTT‑CCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAA‑TGCACGGTACCTACGAAGcta < 2:286820/250‑3 (MQ=255) tACAATCCCACGACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCGGGTGGCGGCAAAAAAACCGGTTGTCGACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAATGGTGGAGGCGTGGAATCTGCCCGTT‑GG‑TTCCGCATT‑GATGGGGCTGGGGGCGTTT‑CCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAA‑TGCACGGTACCTACGAAGCCaa < 1:409868/248‑1 (MQ=255) tACAATCCCAACACTACCGGACATAAAGGTCAAATTAATCGTGCACTTCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGCAGGTGCAATCTCTGCGTGCTTCCATCAGCAGTTGAAATAAATGTTGGATGCGTTGAATCTGCCCGGT‑TT‑TTCCTCATT‑GATGGGTCTGGGGGCGTTT‑CCGGCAACGCATCGTCAGTCACTGGGCATGCTGGGAA‑TTCACTTTACCTACGAAACCAata < 2:442944/250‑1 (MQ=255) aCAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAATGGTGGAGGCGTTGAATCTGCCCGTT‑GT‑TTCCTCATT‑GATGGGGCTGGGGGCGTTT‑CCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAA‑TGCACGGTACCTACGAAGCCAatat < 1:915614/250‑1 (MQ=255) cccACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGAGGGGCAATCACGGCGGGCCGCCATCAGCAGTTGAAAGAAATGGGGGAGGCGTTGAATCTGCCCGTT‑GT‑TTCCTCATT‑GATGGGGCTGGGGGCGCTT‑CCGGCAAAGCATCGTCAGGCAATGGACATGCTGGGGA‑TTCACGGTGCCTACGAAGcccaattggaga > 1:756295/1‑240 (MQ=255) actacCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTAGCGGCAAAAAAACCGGTTGTCTACGTAGGCGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAACGGTGGAGGCGTTGAATCTGCCCGTT‑GT‑TTGCTCATT‑GATGGGGCTGGGGGCGTTT‑CCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAA‑TGCACGGTACCTACGAAGCCAATATGACGATGCAt < 1:492074/249‑1 (MQ=255) acaacCGGGCATAAAGGGCAAATTAAGCGTTCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAATGGTGGAGGCGTTGAATCTGCCCGTT‑GT‑TTCCTCATT‑GATGGGGCTGGGGGCGTTT‑CCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAA‑TGCACGGTACCTACGAAGCCAATATGACGATGCATa < 1:641258/247‑1 (MQ=255) acCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAATGGTGGAGGCGTTGAATCTGCCCGTT‑GT‑TTCCTCATT‑GATGGGGCTGGGGGCGTTT‑CCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGGA‑TGCACGGTACCTACGAAGCCAATATGACGCTGCATAAcgc > 1:725629/1‑251 (MQ=255) cGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAATGGTGGAGGCGTTGAATCTGCCCGTT‑GT‑TTCCTCATT‑GATGGGGCTGGGGGCGTTT‑CCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAA‑TGCACGGTACCTACGAAGCCAATATGACGATGCATAACGCgg > 1:728586/1‑251 (MQ=255) ggACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCGGCCATCAGCAGTTTAAAGAAATGGTGGAGGCGTTGAATCTGCCCGTT‑GT‑TTCCTCATT‑GATGGGGCTGGGGGCGTTT‑CCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGGA‑TGCACGGTACCTCCGAAGCCAATATGCCGATGCAGAACGCggg > 2:23657/1‑250 (MQ=255) aaaGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAATGGGGGGGGGGTTGGATCTGCCCGTT‑GT‑TTCCTCATT‑GATGGGGCGGGGGGCGGTT‑CCGGCAACGCATTGTCCGGCCCTGGGGATGCTGGGAG‑TGCACGGTGACTACGAAGCCCATATGACGATGCCTACAGCGG‑ATGTG‑At > 2:196631/1‑251 (MQ=255) aGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAATGGTGGAGGCGTTGAATCTGCCCGTT‑GT‑TTCCTCATT‑GATGGGGCTGGGGGCGTTT‑CCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAA‑TGCACGGTACCTACGAAGCCAATATGACGATGCATAACGCGG‑ATGTG‑Attt < 1:276517/251‑1 (MQ=255) gCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAATGGGGGAGGCGGTGAATCTGGCCGTT‑GT‑TTCCTCATT‑GATGGGGGTGGGGGGGGTT‑CCGGAAACGCATCGGTAAGCACAGGGCATTCTGGGAA‑TGCAAGGTACCTAAGAAGCCAATATGAAGATTCATAAAGCGGGACGTG‑Atttcg > 1:741424/1‑249 (MQ=255) gCAAATTAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCCGCCATCAGCAGTTTGAAGAAATTGTGGAGGCGGTGAATCTGCCCGTT‑GT‑TTCCTCATT‑GATGGGGCTGGGGGCGTTT‑CCGGCACAGCATCCTTAGGTCCTGGGCCTGCTGGGAA‑TTCACAGGACCTACCAAGCCAATATGACGGCGCATAACGCGG‑GTGGG‑Atttttc > 1:675930/1‑249 (MQ=255) cAAATTAAGCGTGCTCTGCCAACGCTGGTGGCGGCAAAAAAACCGGTTGGCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAAGGGTGGAGGCGTTGAATCTGCCCGTT‑GT‑TTCCGCATT‑GATGGGGCTGGGGGCGTTT‑CCGGCAACGCATCGTCAGGCTCTGGGCATGCTGGGAA‑TGCACGGTACCTACGAAGCCAATATGACGATGCATAACGCGG‑AGGTG‑ATTTTCGc < 2:983012/251‑1 (MQ=255) aaataaagcgggcgcggCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAATGGTGGAGGCGTTGAATCTGCCCGTT‑GT‑TTCCTCATT‑GATGGGGCTGGGGGCGTTT‑CCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAA‑TGCACGGTACCTACGAAGCCAATATGACGATGCATAACGCGG‑ATGTG‑ATTTTCGcc < 2:155158/235‑1 (MQ=255) ttAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCCGCCATCAGCAGTTGAAAGAAATGGTGGAGGCGTTGAATCTGCCCGTT‑GT‑TTCCTCATT‑GATGGGGCTGGGGGGGTTT‑CCGGCAAAGCATCGTCAGGCACTGGGCGTGCTGGGAA‑TGCACGGTACCTACGAAGCCAATATGACGATGCATAACGCGG‑ATGTG‑ATTTTCGCCGTc > 1:467012/1‑251 (MQ=255) tAAGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCATCAGCGGTTGAAAGAAATGGTGGAGGAGTTTAAACTGCCAGTT‑GT‑TTACTCATT‑GATGGGGCGGGGGGGGGTT‑CCGGCGACACATCGTCCGGCACTGGGGATGCGGGGGGGTG‑ACGGTCCCTACGACGCCAATATGAAGATGCTAAACCCGG‑ATGTG‑ATTTTGCCCGTCg > 2:56268/1‑251 (MQ=255) aGCGTGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAATGGTGGAGGCGTTGAATCTGCCCGTT‑GT‑TTCCTCATT‑GATGGGGCTGGGGGCGTTT‑CCGGCAACGCATCGTCAGGCACTTGGCATGCTGGGAA‑TGCACGGTACCTACGAAGCCAATATGACGATGCATAACGCGG‑GTGTG‑ATTTTCGCCGTCggg > 1:328614/1‑251 (MQ=255) tGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAATGGTGGAGGCGTTGAATCTGCCCGTT‑GT‑TTCCTCATT‑GATGGGGCTGGGGGCGTTT‑CCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAA‑TGCACGGTACCTACGAAGCCAATATGACGATGCATAACGCGG‑ATGTG‑ATTTTCGCCGTCGGGGTAc > 1:18844/1‑251 (MQ=255) ggCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAATGGTGGAGGCGTTGAATCTGCCCGTT‑GT‑TTCCTCATT‑GATGGGGCTGGGGGCGTTT‑CCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAA‑TGCACGGTACCTACGAAGCCAATATGACGATGCATAACGCGG‑ATGTG‑ATTTTCGCCGTCGGGGTAc < 2:340244/250‑1 (MQ=255) gCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAATGGTGGAGGCGTTGAATCTGCCCGTT‑GT‑TTCCTCATT‑GATGGGGCTGGGGGCGTTT‑CCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAA‑TGCACGGTACCTACGAAGCCAATATGACGATGCATAACGCGG‑ATGTG‑ATTTTCGCCGTCGGGGTAC‑g < 1:300362/251‑1 (MQ=255) tctGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAATGGTGGAGGCGTTGGATCTGCCCGTT‑GT‑TTCCTCATT‑GATGGGGCTGGGGGCGTTT‑CCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAA‑TGCACGGTACCTACGAAGACAATATGACGATGCATAACGCGG‑ATGTG‑ATTTTCGCCGTCGGGGTAC‑GAt > 1:183752/1‑251 (MQ=255) tGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAATGGTGGAGGCGTTGAATCTGCCCgt < 1:418757/112‑1 (MQ=255) ggCAAACGCGGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAATGGTGGAGGCGTTGAATCTGCCCGTT‑GT‑GTCCTCATT‑GATGGGGCTGGGGGCGTTT‑CCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAA‑TGCACGGTACCTACGAAGCCAATATGACGATGCATAACGCGG‑ATGTG‑ATTTTCGCCGTCGGGGTAC‑GAttt < 1:627887/250‑1 (MQ=255) gCAAACGCTTGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTTGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAATGGTGGAGGCGTTGAATCTGCCCGTG‑GT‑TTCCTCATT‑GATGGGGCTGGGGGCGTTT‑CCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAA‑TGCACGGTACCTACGAAGCCAATATGACGATGCATAACGCGG‑ATGTG‑ATTTTCGCCGTCGGGGTAC‑GAttt < 1:651497/250‑1 (MQ=255) gCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAATGGTGGAGGCGGTGAATCTGCCCGTT‑GT‑TTCCTCCTT‑GATGGGGCTGGGGGCGTTT‑CCGGCAACGCATCGTCAGGCACTGGGCAAGCTGGGAA‑TTCACGGTACCTAAGAAGCCAAAATGACGATGCATAACGCGG‑ATGGG‑Atttttgcagacggggacagatttt > 1:433186/1‑231 (MQ=255) aaaCGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCTGGATGCCATCAGCAGTTGAAAGAAATGGTGGAGGCGTTGCATCTGTCCGTT‑GT‑TTACTTATT‑GCTGGGGCTGGGGGCGTTT‑CCCGCAACGCATCGTCACGCACGGGGCATGCTGGGTA‑TGCACGGTACCTACGAAACCAATATGGCGATGCATAACGCGG‑ATGTG‑ATTTTCGCCGTAGGGGTAT‑GAtttaat > 1:606939/1‑248 (MQ=255) gtggCGGCAAAAAAACCGGTTGTCTACGTAGGTGGTGGGGCAATCCCGGCGGGCTGCCATCAGCAGTTGAAAGAAATGGTGGAGGCGTTGAATCTGCCCGTT‑GT‑TTCCTCATT‑GATGGGGCTGGGGGCGTTT‑CCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAA‑TGCACGGTACCTACGAAGCCAATATGACGATGCATAACGCGG‑ATGTG‑ATTTTCGCCGTCGGGGTAC‑GATTTGATGACCGAac < 2:183752/248‑1 (MQ=255) cAAAAAAACCGGTTGTCTACTTGGGTGGTGTGGCAATCACGGCGGGCTGCACTCAGCAGTTGAAAGAAATGGTGGAGGCGGTGAATCTGCCCGTT‑GT‑TTCCTCATT‑GATGGGGCTGGGGGCGTTT‑CCGGCAACGAATCGTCAGGCACTGGGCATGTTGGGAA‑TGCACGGTACCTACGAAGCCAATATGACGATGCATAACGCGT‑ATGTG‑ATTTTCGCCGGCGGGGTTC‑GATTTGATGACCGAacgactaa < 2:717076/250‑4 (MQ=255) aaCCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAATGGTGGAGGCGTTGAATCTGCCCGTT‑GT‑TTCCTCATT‑GATGGGGCTGGGGGCGTTT‑CCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAA‑TGCACGGTACCTACGAAGCCAATATGACGATGCATAACGCGG‑ATGTG‑ATTTTCGCCGTCGGGGTAC‑GATTTGATGACCGAACGACGAACAATCTg < 1:23657/251‑1 (MQ=255) aaCCGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTGGAAAGAAATGGTGGAGGCGTGGAATCTGCCCGTT‑GT‑TTCCTCATT‑GATGGGGCTGGGGGCGTTT‑CCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAA‑TGCACGGTACCTACGAAGCCAATATGACGATGCATAACGCGG‑ATGTG‑ATTTTCGCCGTCGGGGTAC‑GATTTGATGACCGAACGACGAACAAtatg < 2:442968/251‑4 (MQ=255) ccGGTTGTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCATCCGCCGTTGAAAGAAATGGTGGAGGCGTTGAATCTGCCCGTT‑GT‑TTCCTCATT‑GATGGGGCTGGGGGCGTTT‑CCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAA‑TGCACGGTACCTACGAAGCCAATATGACGATGCATAACGCGG‑ATGTG‑ATTTTCGCCGTCGGGGTAC‑GGTTTGATGACCGAACGACGAACAATCTGGc > 1:890183/1‑251 (MQ=255) gTCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTTAAAGACATGGTGGAGGCGTTGGATCGGCCCGTTTGT‑TT‑CTCAAT‑GATGGGGCGGGGGGAGTTT‑TCAGCAACGCCTCGTCAAGCACTGGGCCTGCTGGGGA‑TGCACGGGACCCACGAATCCAACATGACGGTGCATAACG‑GG‑GGGTGGATTTTCAGCGTCGGGGGACATATTTG‑TGACCGAACGCAGAAAAATCTGGCCA‑AGtg > 2:516390/1‑250 (MQ=255) tCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCATCAGCCGTTGAAAGAAATGGGGGAGGGGTTGAATCTGCCCGTT‑GT‑TTCCTCATT‑GATGGGGCTGGGGGGGTTT‑CCGGCAAAGCATCGTCAGGCACTGGGCATGCTGGGAA‑TTCACGGTACCTACGAAGACAATATGACGAAGCATAAAGCGG‑GTGTG‑ATTTTTCCCGTAGGGGTAC‑GGATTGATGACCCGACGGCGGACAAACTGGCAA‑AGTAc > 2:982157/1‑251 (MQ=255) tCTACGTAGGTGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAATGGTGGAGGCGTTGAATCTGCCCGTT‑GT‑TTCCTCATT‑GATGGGGCTGGGGGCGTTT‑CCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAA‑TGCACGGTACCTACGAAGCCAATATGACGATGCATAACGCGG‑ATGTG‑ATTTTCGCCGTCGGGGTAC‑GCTTTGATGACCGAACGACGAACAATCTGGCAA‑AGTAc > 1:953649/1‑251 (MQ=255) tAGGTGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAATGGTGGAGGCGTTGAATCTGCCCGTT‑GT‑TTCCTCATT‑GATGGGGCTGGGGGCGTTT‑CCGGCAACGCATCGTCAGGCACTGGGCAAGCTGGGAA‑TGCACGGTACCTACGAAGCCAATATGACGAAGCATAACGCGG‑ATGTG‑GTTTTCGCCGTCGGGGGAC‑GGATTGGTGACCGAACGACGAACAAACTGGCAA‑AGTAATGc‑cc > 1:209999/1‑250 (MQ=255) aggtggTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAATGGTGGAGGCGTTGAATCTGCCCGTT‑GT‑TTCCTCATT‑GATGGGGCTGGGGGCGTTT‑CCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAA‑TGCACGGTACCTACGAAGCCAATATGACGATGCATAACGCGG‑ATGTG‑ATTTTCGCCGTCGGGGTAC‑GATTTGATGACCGAACGACGAACAATCTGGCAA‑AGTACTGC‑CCaa < 1:403617/247‑1 (MQ=255) ggggCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAATGGTGGAGGCGTTGAATCTGCCCGTT‑GT‑TTCCTCATT‑GATGGGTCTGGGGGCGTTT‑CCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAA‑TGCACGGTACCTACGAAGCCAATATGACGATGCATAACGCGG‑ATGTG‑ATTTTCGCCGTCGGGGTAC‑GATTGGATGACCGAACGACGAACAATCTGGCAA‑AGTACTGC‑CTAAATGCCAc < 1:368166/251‑1 (MQ=255) ggCAATCACGGCGGGTTGCCATCAGCAGTTGAAAGAAATGGTGGAGGCGTTGAATCTGCCCGTT‑GT‑TTCCTCATT‑GATGGGGCTGGGGGCGTTT‑CCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAA‑TGCACGGTACCTACGAAGCCAATATGACGATGCATAACGCGG‑ATGTG‑ATTTTCGCCGTCGGGGTAC‑GATTTGATGACCGAACGACGAACAATCTGGCAA‑AGTACTGC‑TCAAATGCCACTg < 2:467012/251‑1 (MQ=255) ggCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAATGGTGGAGGCGTTGAATCTGCCCGTT‑GT‑TTCCTCATT‑GATGGGGCTGGGGGCGTTT‑CCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAA‑TGCACGGTACCTACGAAGCCAATATGACGATGCATAACGCGG‑ATGTG‑ATTTTCGCCGTCGGGGTAC‑GATTTGATGACCGAACGACGAACAATCTGGCAA‑AGTACTGc > 1:639705/1‑238 (MQ=255) ggCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAATGGTGGAGGCGTTGAATCTGCCCGTT‑GT‑TTCCTCATT‑GATGGGGCTGGGGGCGTTT‑CCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAA‑TGCACGGTACCTACGAAGCCAATATGACGATGCATAACGCGG‑ATGTG‑ATTTTCGCCGTCGGGGTAC‑GATTTGATGACCGAACGACGAACAATCTGGCAA‑AGTACTGc < 2:639705/238‑1 (MQ=255) aaTCACGGCGGGCTGCCATCAGCAGTTGAAAGAAATGGTGGAGGCGTTGAATCTGCCCGTT‑GT‑TTCCTCATT‑GATGGGGCTGGGGGCGTTT‑CCGGCAACTCATCGTCAGGCACTGGGCATGCTGGGAA‑TGCACGGTACCTACGAAGCCAATATGACGATGTATAACGCGG‑ATGTG‑ATTTTCGCCGTCGGGGTAC‑GATTTGATGACCGAACGACGAACAATCTGGCAA‑AGTTCTGC‑TCAAATGCCACTGTTc < 2:580710/251‑1 (MQ=255) aaTCACGGCGGGCTGCCATCAGCAGTGGAAAGAAATGGTGGAGGCGTTGAATCTGCCCGTT‑GT‑TTCCTCATT‑GATGGGGCTGGGGGCGTTT‑CCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAA‑TGCACGGTACCTACGAAGCCAATATGACGATGCATAACGCGG‑ATGTG‑ATTTTCGCCGTCGGGGTAC‑GATTTGATTACCGAACGACGAACAATCTGGCAA‑AGTACTGC‑CCAAATGCCACTGtgc < 1:571576/251‑3 (MQ=255) gcACGGCGGGCTGCCATCAGCAGTTGAAAGAAATGGTGGAGGCGTTGAATCTGCCCGTT‑GT‑TTCCTCATT‑GATGGGGCTGGGGGCGTTT‑CCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAA‑TGCACGGTACCTACGAAGCCAATATGACGATGCATAACGCGG‑ATGTG‑ATTTTCGCCGTCGGGGTAC‑GATTTGATGACCGAACGACGAACAATCTGGCAA‑AGTACTGC‑CCAAATGCCACTGTTCTg < 1:850973/250‑1 (MQ=255) cACGGCGGGCTGCCATCAGCAGTTGAAAGAAATGGTGGAGGCGTTGAATCTGCCCGTT‑GT‑TTCCTCATT‑GATGGGGCTGGGGGCGTTT‑CCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAA‑TGCACGGTACCTACGAAGCCAATATGACGATGCATAACGCGG‑ATGTG‑ATTTTCGCCGTCGGGGTAC‑GATTTGATGACCGAACGACGAACAATCTGGCAA‑AGTACTGC‑CCAAATGCCACTGTTCTg < 1:762474/250‑1 (MQ=255) aCGGCGGGCGGCCATCAGCAGTTGAAAGAAATGGGGGAGGCGTTGAATCTGCCCGTT‑GT‑TTCCGCATT‑GATGGGGCTGGGGGCGTTT‑CCGGCAACGCAGCGTCAGGCACTGGGCATGCTGGGAA‑TGCACGGTACCTACGAAGCCAATATGACGATGCATAACGCGG‑ATGTG‑ATTTTCGCCGTCGGGGTAC‑GATTTGATGACCGAACGACGAACAATCTGGCAA‑AGTACTGC‑CCAAATGCCACTGTTCTGCa < 2:374294/251‑1 (MQ=255) gcggGCTGCCATCAGCAGTTGAAAGAAATGGTGGAGGCGTTGAATCTGCCCGTT‑GT‑TTCCTCATT‑GATGGGGCTGGGGGCGTTT‑CCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAA‑TGCACGGTACCTACGAAGCCAATATGACGATGCATAACGCGG‑ATGTG‑ATTTTCGCCGTCGGGGTAC‑GATTTGATGACCGAACGACGAACAATCTGGCAA‑AGTACTGC‑CCAAATGCCACTGTTCTGCatat < 1:404872/251‑1 (MQ=255) cggGCTGCCATCAGCAGTTGAAAGAAATGGTGGAGGCGTTGAATCTGCCCGTT‑GT‑TTCCTCATT‑GATGGGGCTGGGGGCGTTT‑CCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAA‑TGCACGGTACCTACGAAGCCAATATGACGATGCATAACGCGG‑ATGTG‑ATTTTCGCCGTCGGGGTAC‑GATTTGATGACCGAACGACGAACAATCTGGCAA‑AGTACTGC‑CCAAATGCCACTGTTCTGCATATc > 1:405004/1‑251 (MQ=255) gggCTGCCATCAGCAGTTGAAAGAAATGGTGGAGGCGTTGAATCTGCCCGTT‑GT‑TTCCCCATT‑GATGGGGCTGGGGGCGTTT‑CCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAA‑TGCACGGTACCTACGAAGCCAATATGACGATGCATAACGCGG‑ATGTG‑ATTTTCGCCGTCGGGGTAC‑GATTTGATGACCGAACGACGAACAATCTGGCAA‑AGTACTGC‑CCAAATGCCACTGTTCTGCATATCg < 1:746633/251‑1 (MQ=255) gggCTGCCAGCAGCAGTTGAAAGAAATGGTGGAGGCGTTGAATCTGCCCGTT‑GT‑TTCCTCATT‑GATGGGGCTGGGGGCGTTT‑CCGGCAACGCATCGTCATGCACTGGGCATGCTGGGAA‑TGCACGGTACCTACGAAGCCAATATGACGATGCATAACGCGG‑ATGTG‑ATTTTCGCCGTCGGGGTAC‑GATTTGATGACCGAACGACGAACAATCTGGCAA‑AGTCCTGC‑CCAAACGCCACTGTTCTGCATATCg < 2:960323/251‑1 (MQ=255) gCTGCCATCAGCAGTTGAAAGAAATGGTGGAGGCGTTGAATCTGCCCGTT‑GT‑TTCCTCATT‑GATGGGGCTGGGGGCGTTT‑CCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAA‑TGCACGGTACCTACGAAGCCAATATGACGATGCATAACGCGG‑ATGTG‑ATTTTCGCCGTCGGGGTAC‑GATTTGATGACCGAACGACGAACAATCTGGCAA‑AGTACTGC‑CCAAATGCCACTGTTCTGCATATCGat < 1:166484/251‑1 (MQ=255) gCCATCAGCAGTTGAAAGAAAGGGGGTAGGCGTTGAATCTGCCCGTT‑GT‑TTCCTCATT‑GATGGGGCTGGGGGCGTTT‑CCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAA‑TGCACGGTACCTACGAAGCCAATATGACGATGCATAACGCGG‑ATGTG‑ATTTTCGCCGTCGGGGTAC‑GATTTGATGACCGAACGACGAACAATCTGGCAA‑AGGACTGC‑CCAAATGCCACTGTTCTGCATATCGatat < 2:655358/250‑1 (MQ=255) aTCAGCATTTGAAAGAAATGGTGGAGGCGTTGAATCTGCCCGTT‑GT‑TTCCTCATT‑GATGGGGCTGGGGGCGTTT‑CCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAA‑TGCACGGTACCTACGAAGCCAATATGACGATGCATAACGCGG‑ATGTG‑ATTTTCGCCGTCGGGGTAC‑GATTTGATGACCGAACGACGAACAATCTGGCAA‑AGTACTGC‑CCAAATGCCACTGTTCTGCATATCGATATTGAt < 1:743275/251‑1 (MQ=255) cagcagTTGAAAGAAATGGTGGAGGCTTTTAATCTGCCCGTT‑GT‑TTCCTTATT‑GATGGGGCTGGGGGCGTTT‑CCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAA‑TGCACGGTACCTACGAAGCCAATATGACGATGCATAACGCGG‑ATGTG‑ATTTTCGCCGTCGGGGTAC‑GATTTGATGACCGAACGACGAACAATCTGGCAA‑AGTACTGC‑CCAAATGCCACTGTTCTGCATATCGATATTGATc < 2:230398/250‑1 (MQ=255) cagTTGAAAGAAATGGTGGAGGCGTTGAATCTGCCCGTT‑GT‑TTCCTCATT‑GATGGGGCTGGGGGCGTTT‑CCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAA‑TGCACGGTACCTACGAAGCCAATATGACGATGCATAACGCGG‑ATGTG‑ATTTTCGCCGTCGGGGGAC‑GATTTGATGACCGAACGACGAACAATCTGGCAA‑AGTACTGC‑CCAAATGCCACTGTTCTGCATATCGATATTGATCCTAc > 1:824065/1‑251 (MQ=255) agTTGAAAGAAATGGTGGAGGCGTTGAATCTGCCCGTT‑GT‑TTCCTCATT‑GATGGGGCTGGGGGCGTTT‑CCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAA‑TGCACGGTACCTACGAAGCCAATATGACGATGCATAACGCGG‑ATGTG‑ATTTTCGCCGTCGGGGTAC‑GATTTGATGACCGAACGACGAACAATCTGGCAA‑AGTACTGC‑CCAAATGCCACTGTTCTGCATATCGATATTGATCCTACt > 1:325051/1‑251 (MQ=255) gTTGAAAGAAATGGTGGAGGCGTTGAATCTGCCCGTT‑GT‑TTCCTCATT‑GATGGGGCTGGGGGCGTTT‑CCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAA‑TGCACGGTACCTACGAAGCCAATATGACGATGCATAACGCGG‑ATGTG‑ATTTTCGCCGTCGGGGTAC‑GATTTGATGACCGAACGACGAACAATCTGGCAA‑AGTACTGC‑CCAAATGCCACTGTTCTGCATATCGATATTGATCCTACtt < 1:864979/251‑1 (MQ=255) tGAAAGAAATGGTGGAGGCGTTGAATCTGCCCGTT‑GT‑TTCCTCATT‑GATGGGGCTGGGGGCGTTT‑CCGGCAACGCCTCGTCAGGCACTGGGCATGCTGGGAA‑TGCACGGTACCTACGAAGCCAATATGACGATGCATAACGCGG‑ATGTG‑ATTTTCGCCGTCGGGGTAC‑GATTTGATGACCGAACGACGAACAATCTGGCAA‑AGTACTGC‑CCAAATGCCACTGTTCTGCATATCGATATTGATCCTACTTcc > 1:635061/1‑251 (MQ=255) gaaagaaaTGGTGGGGGCGTTGAATCTGCCCGTT‑GT‑TTCCTCATT‑GATGGGGCTGGGGGCGTTT‑CCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAA‑TGCACGGTACCTACGAAGCCAATATGACGATGCATAACGCGG‑ATGTG‑ATTTTCGCCGTCGGGGTAC‑GATTTGATGACCGAACGACGAACAATCTGGCAA‑AGTACTGC‑CCAAATGCCACTGTTCTGCATATCGATATTGATCCTACTTcc > 1:741694/1‑250 (MQ=255) gaaagaaaTGGTGGAGGCGTTGAATCTGCCCGTT‑GT‑TTCCTCATT‑GATGGGGCTGGGGGCGTTT‑CCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAA‑TGCACGGTACCTACGAAGCCAATATGACGATGCATAACGCGG‑ATGTG‑ATTTTCGCCGTCGGGGTAC‑GATTTGATGACCGAACGCCAGACAACCTGGCAA‑AGTACTGC‑CCAAATGCCACTGTTCTGCCTATCGATATTGATCCTACTTccc > 2:146853/1‑250 (MQ=255) aagaaaTGGTAGAGGCGATGAATATGCCCGTT‑GA‑TTCATCATT‑GATGGGGCTGGGGGCGTTT‑GCGGCAAAGAATCGACAGGCACTGGGCATGCTGGGAA‑TGCACGGTACCTACGAAGCCAATATGACGATGCATACAGCGG‑AAGTG‑ATTTTCGCCGTCGGGGAGC‑GATTTGATGACCGAACGACGAAAACTCTGGCAACAG‑ACTGCACCAAA‑GCCACGGTTCTGCATATCAATATTGATCCTAATTCCAtt > 2:842712/1‑251 (MQ=255) | GCTGGCGGCAAGTGGTCGCCCAGGACCAGTAGTCGTTGATTTACCGAAAGATATTCTTAATCCGGCGAACAAATTACCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTACAATCCCACTACTACCGGACATAAAGGGCAAATTAAGCGTGCTCTGCAAACGCTGGTAGCGGCAAAAAAACCGGTTGTCTACGTAGGCGGTGGGGCAATCACGGCGGGCTGCCATCAGCAGTTGAAAGAAACGGTGGAGGCGTTGAATCTGCCCGTT‑GT‑TTGCTCATT‑GATGGGGCTGGGGGCGTTT‑CCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAA‑TGCACGGTACCTACGAAGCCAATATGACGATGCATAACGCGG‑ATGTG‑ATTTTCGCCGTCGGGGTAC‑GATTTGATGACCGAACGACGAACAATCTGGCAA‑AGTACTGC‑CCAAATGCCACTGTTCTGCATATCGATATTGATCCTACTTCCATT > NC_000913/86070‑86559 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |