Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	65,208	A→G	L191L (CTT→CTC)	polB ←	DNA polymerase II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	65,208	0	A	G	99.0%	304.2 / NA	96	L191L (CTT→CTC)	polB	DNA polymerase II
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (49/46);  minor base T (0/1);  total (49/47)
Fisher's exact test for biased strand distribution p-value = 4.90e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGCCGACCTTTAGCCTGGGCAAAAAA‑GACG‑CCGTTTTTAAAGCCGTGCTCGCGCC‑ACTCCAGCTCGCTATTATCGCGCCCAAGACGCAGCGGAAGACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGCAACTGCGGGCGGCTGGCGACGTATTCCAGTTCGAAATCAAGCGAGGAGGCGTCGCCATTCTCCGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAATATCTA‑TAGAAACCCACTTGAGCGGCGGA‑CGATAGT‑CGGGATGCGGTTTCAGACGGGCATTAACGATAGTGCCATTGTGCA‑TATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCTCCATCAGATAGCGT  >  NC_000913/64963‑65445                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                  aGCCGACCTTTAGCCTGGGCAAAAAA‑GCCG‑CCGTTTTTAAAGCCGTGCTCGCGCC‑ACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCgc                                                                                                                                                                                                                                              >  1:330562/1‑250 (MQ=255)   ccGACCTTTGGCCGGGGCAAAAAATGA‑G‑CCGTTTTTAAAGCAGTGCCCGCGCC‑ACGCCAGCTCACTGTTAACGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTGCCAACCGATGATCACAGCAGGATCTTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGGAGCTGCGGGCGTCTGGCGACGTATTCCAGTTTAAAATCGAGCgcgg                                                                                                                                                                                                                                            <  2:97478/251‑4 (MQ=255)    cGACCTTTAGCCTGGGCAAAAAA‑GACG‑CCGTTTTTAAAGCCGTGCTCGCGCC‑ACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCgcgga                                                                                                                                                                                                                                           <  1:228499/251‑1 (MQ=255)          ttaaccTGGGCAAAAAA‑GACG‑CCGTTTTTAAAGCCGTGCTCGCGCCCAC‑CCAGCTCACGGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCg                                                                                                                                                                                                                                       <  2:462254/245‑1 (MQ=255)             cccTGGGAAAAAAA‑G‑CGTCCGTTTTTAAAGCCTTGCTCGCGCC‑CCTCCATCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGGTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTGCAAAATCGAGCGCGGATGCGTCGcc                                                                                                                                                                                                                                  <  2:478131/250‑1 (MQ=255)                 gggCAAAAAA‑GACG‑CCGTTTTTAAAGCCGTGCTCGCGCC‑ACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAAACAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGACGCCATtc                                                                                                                                                                                                                              >  1:731196/1‑251 (MQ=255)                   gCAAAAAA‑GACG‑CCGTTTTTAAAGCCGTGCTCGCGCC‑ACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATtctc                                                                                                                                                                                                                            >  2:515522/1‑251 (MQ=255)                      aaaaa‑GACG‑CCGTTTTTAAAGCCGTGCTCGCGCC‑ACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATtctctgg                                                                                                                                                                                                                         >  1:599190/1‑248 (MQ=255)                         aa‑GACG‑CCGTTTTTAAAGCCGTGCTCGCGCC‑ACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGccc                                                                                                                                                                                                                      >  2:227137/1‑251 (MQ=255)                                 ccGTTTTTAAAGCCGTGCTCGCGCC‑ACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCCTGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGCGGCGGCCCCATTATCTGGCCCCAGcct                                                                                                                                                                                                                >  2:672799/1‑249 (MQ=255)                                    tttttAAAGCCGTGCTCGCGCC‑ACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCatat                                                                                                                                                                                                              >  1:516656/1‑250 (MQ=255)                                    tttttAAAGCCGTGCTCGCGCC‑ACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGCAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCatat                                                                                                                                                                                                              >  1:447486/1‑250 (MQ=255)                                      tttAAAGCCGTGCTCGCGCC‑ACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATaa                                                                                                                                                                                                            >  1:288511/1‑250 (MQ=255)                                         aaaGCCGTGCTCGCGCC‑ACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGAATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCCCTGGCCCCAGCCTACAAACg                                                                                                                                                                                                         >  2:948984/1‑250 (MQ=255)                                             ccGTGCTCGCGCC‑ACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGACGCCCTTCTCTGGCCCCAGCATAAAAACGATggg                                                                                                                                                                                                    >  2:542203/1‑249 (MQ=255)                                                tGCTCGCGCC‑ACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTg                                                                                                                                                                                                 >  1:213774/1‑251 (MQ=255)                                                 gcactcgcC‑CCTCCATCTCACTGTTATCTCGCCCCAGCCGCAGCTGAATACTGTAACGCTCGGCATGTTTTTGCAGCATTCGCATATCGAACTGCACCCCGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAGCGATGCGCTg                                                                                                                                                                                                 <  1:212058/245‑1 (MQ=255)                                                   ccgcgCC‑CCTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGccc                                                                                                                                                                                              <  1:104840/250‑1 (MQ=255)                                                    cgcgCC‑ACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCg                                                                                                                                                                                             <  1:746808/251‑1 (MQ=255)                                                    cgcgCC‑ACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCg                                                                                                                                                                                             >  1:868290/1‑251 (MQ=255)                                                       gcC‑ACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAg                                                                                                                                                                                          <  1:892895/251‑1 (MQ=255)                                                         c‑ACTCCAGCTCACTGTTATAGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCAGATGGCGACGTAATCCAGTTCAAAAACGAGCGCGGAGGCGTCGCCATTCGCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGcc                                                                                                                                                                                        >  2:570391/1‑251 (MQ=255)                                                            cTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATACACGATGCGCAGCCCGCATCCtt                                                                                                                                                                                      >  2:820948/1‑251 (MQ=255)                                                              ccagcgcactgttatCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCtt                                                                                                                                                                                      <  1:297300/238‑1 (MQ=255)                                                              ccAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCTGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCtt                                                                                                                                                                                      <  1:429466/249‑1 (MQ=255)                                                              ccAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGGAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCtt                                                                                                                                                                                      <  1:86245/249‑1 (MQ=255)                                                               cagccccctgttatCGCCCCCCAGCCGCATCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGACGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCa                                                                                                                                                                                   <  2:288511/241‑1 (MQ=255)                                                               cAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCa                                                                                                                                                                                   <  1:908327/251‑1 (MQ=255)                                                                   tcactgttatCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGcc                                                                                                                                                                               >  2:235742/7‑251 (MQ=255)                                                                      ctgttatCGCGCCCCAGACGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCACCCGATGATCACATCGGGATCGTAGTTGGCAAACCCGGCGTTGAGTTTTTCCAATAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCACAATCGCGCGCGGAGGCCTCGCCCTTCTCTGGCACCAGAATTTACACGATCCGCTACCCGACGCCTTCAAGGCCGAt                                                                                                                                                                            >  2:581515/4‑251 (MQ=255)                                                                        gttatCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGAt                                                                                                                                                                            <  1:235742/248‑1 (MQ=255)                                                                             cgcgCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGAGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTAc                                                                                                                                                                     >  1:728617/1‑251 (MQ=255)                                                                                gccccagccGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGAAGCCTTCCAGGCCGATGCAGTACAGc                                                                                                                                                                  >  2:787542/9‑251 (MQ=255)                                                                                   ccagccGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTc                                                                                                                                                                >  1:527515/6‑250 (MQ=255)                                                                                    cagccGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACCGCTCAc                                                                                                                                                              >  1:325352/5‑251 (MQ=255)                                                                                           aGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCg                                                                                                                                                       >  1:118419/1‑251 (MQ=255)                                                                                               gaatccgtgtaCGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGGTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATTCAGTACAGCTCACCGTGGCGggtg                                                                                                                                                   <  2:213774/241‑1 (MQ=255)                                                                                               gaataCGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGATGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGggtg                                                                                                                                                   <  1:807709/247‑1 (MQ=255)                                                                                                    cGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTACAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGCCCGATGCAGTACAGCTCACCGTGGCGGGGGGtttt                                                                                                                                              >  2:67137/1‑250 (MQ=255)                                                                                                         aCGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTTGTTTCAatat                                                                                                                                         >  1:283723/1‑251 (MQ=255)                                                                                                            cTCGGCATGTTTTTGCAGCCTTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGGGTTTTTCCAGTAGCTGCGGGCGGCTGGCGCCGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGCTGCAGTACCGCTCACCAGGGCGGGGGGTGTCAatataa                                                                                                                                       >  2:629644/1‑248 (MQ=255)                                                                                                              cGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCCGTACAGCTCACCGTGGCGGGAGGGTTCAATATcaaat                                                                                                                                    >  2:81235/1‑247 (MQ=255)                                                                                                                 cctGTTTTTGCAGCATTCGCAGATCGAACTGCACCACTTTCCAACTGATGATCTAATCAGGCTCGTAGTTGGCCGACCAGGCGTTGAGTTTTTCCAGTCGCTGCGGTCGGCTGGCGACGTATTCCAGTTCTAAATCGAGAGCGGAGGCGTCGCCATTCTCTGGCCCACCCATATAACCGATGCTCTGCCCGCCGCCTTACAGTCCCATGCAGTACAGCTCACCTTGACGgttggtctctatctacaaggg                                                                                                                                  >  2:914169/3‑235 (MQ=255)                                                                                                                 cATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTCCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTTGTTTCAATATCAA‑TAGa                                                                                                                                 >  1:341309/1‑250 (MQ=255)                                                                                                                    gTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGGTTCAATATcaaaaaaaacc                                                                                                                              >  1:674666/1‑241 (MQ=255)                                                                                                                        tggtagcaTTCGCAGATCGAATTGCCCCACGTGCCAACCGATTGTCACCTCAGGCTCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGGAGCTGCGTGCGGCTGGCGATGGTTTCCAGTCCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGTTGCCCGCAGCCTTCCAGGCCGATGCAGGACAGCTCACCGTGGCTGGTGGTTTCAATATCAA‑TAGACACCCAc                                                                                                                          <  2:824414/246‑1 (MQ=255)                                                                                                                         tGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAATATCAA‑TAGAAACCCACt                                                                                                                         <  1:651521/250‑1 (MQ=255)                                                                                                                         tGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGGGGTTTCAATATCTA‑AAGAAACCCACtt                                                                                                                        >  1:290196/1‑251 (MQ=255)                                                                                                                           cagcaTTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGGGGTTTCAATATCAA‑TAAAAACCCAcatg                                                                                                                       >  2:610936/1‑247 (MQ=255)                                                                                                                            agcaTTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGGGGTTTCAATATCAA‑TAGAAACCCACTTg                                                                                                                       >  1:806150/1‑249 (MQ=255)                                                                                                                                ttCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGGGGGTTCAATATCAA‑TAGAAAACCACTTGAgcggg                                                                                                                 >  1:358069/1‑250 (MQ=255)                                                                                                                                    gaGATCGAACTGCACCCGGTTGCATCCGATGATCACATCAGGATCGTAGTTGGCAAACCAGTCTTTGAGTTTTTCCAGGAGCTGCGGGCGTCTGGCGACTTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCACACCGTGGCGGGTGGTTTCAATATCAA‑TAGAAACCCACTTTAGCGGCGGA‑c                                                                                                            <  2:72330/250‑1 (MQ=255)                                                                                                                                     aGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAATATCAA‑TAGAAACCCACTTGAGCGGCGGA‑Cg                                                                                                           <  1:809482/251‑1 (MQ=255)                                                                                                                                      gATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAATATCAA‑TAGAAAACCACTTAAGcggcgggcgg                                                                                                           >  1:79113/1‑247 (MQ=255)                                                                                                                                         cGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGCGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGGGGCGGGGGGGTTCTATATCAAATAAAAAACCA‑TTGAGcggcgggggaa                                                                                                          >  2:523670/1‑244 (MQ=255)                                                                                                                                               gCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAATATCAA‑TAGAAACCCACTTGAGCGGCGGA‑AGATAGT‑Cgggg                                                                                                >  1:268797/1‑250 (MQ=255)                                                                                                                                                caccacGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGGTTCAATATCAA‑AAGAAACCCACTTGAGCGGCGGA‑CGGTAGT‑Cggg                                                                                                 >  1:582609/1‑249 (MQ=255)                                                                                                                                                caccacGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGGGGGTTCAATATCCA‑TAGAAACCCCCTTGAGCGGCGGG‑CGATAGT‑Cggggg                                                                                               >  1:838653/1‑249 (MQ=255)                                                                                                                                                 aacacGTTCCAACCGATGATCACATCAGGATCGTGGTTGGCAATACAGGCGGTGAGGTTTCCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCGCACCGTGGCGGGTGGTTTCAATATCAA‑TAGAAACCCACTTGAGCGGCGGA‑CGATAGT‑CGGGATg                                                                                              <  2:316078/249‑1 (MQ=255)                                                                                                                                                  ccacGTTCCAACCGAGGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCTATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAATATCAA‑TAGAAACCCACTTGAGCGTCGGA‑CGATAGT‑CGGGATGc                                                                                             <  1:581017/251‑1 (MQ=255)                                                                                                                                                   cacgtgccaccctagggtcccgacAGGATCGTAGTTGGCAAACCTGGGGTTGAGTCTTTCCAGGAGCTGCGGGCGGCTGGCGACGTATTCCAGTGCAAAAGCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCTGCCTTCCAGGCCTATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAATATCTA‑TAGAAACCCACTTGAGCGGCGGA‑CGATAGT‑CGGGATGct                                                                                            <  2:994266/228‑2 (MQ=255)                                                                                                                                                       ttCCAACCGATGATCACCTCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCCAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGGGGGTTAAATCTCGG‑TAGAAAACCACGTTAGAGGCGGG‑CGGTAGT‑Cggggtgggggtt                                                                                        >  1:108069/1‑242 (MQ=255)                                                                                                                                                        tCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGGGGCGGGTGGGTTCAATATCCA‑TAGAAACACAATTGTGAGGGGGG‑CGATAGT‑CGGGGTGGGGtttt                                                                                       >  2:713780/1‑250 (MQ=255)                                                                                                                                                         ccAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAATATCAA‑TAGAAACCCACTTGAGCGGCGGA‑CGATAGT‑CGGGATGCGGTTTCa                                                                                      <  1:787542/251‑1 (MQ=255)                                                                                                                                                          cAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCTGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGTCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAATATCAA‑TAGAAACACACTTGAGCGGCGGA‑CGATAGT‑CGGGATGCGGTTTCAg                                                                                     <  1:848132/251‑1 (MQ=255)                                                                                                                                                               gatgataccaTCAGGACCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGGCGCCATTCTCTGGCCCCAGCATAGAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTGCAATATCAA‑TAGAAACCCACTTGAGCGGCGGA‑CGATAGT‑CGGGATGCGGTTTCAGACggg                                                                                <  2:868290/243‑1 (MQ=255)                                                                                                                                                                 tgatCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCCGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGCTGCGCTGCCCGCAGCCTTCCCGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCCATCTCAA‑TAAAAACCCCCTTGCGCGGAGGA‑CGGTTAT‑CGGGATGCGGTTTCCGACGGGc                                                                               >  1:179979/1‑250 (MQ=255)                                                                                                                                                                  gatCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGGTTCAATATAAA‑TAGAAACCCACTTGAGCGGCGGG‑CGATAGT‑CGGGGTGCGGGTTCAGGAGGGCAt                                                                             >  1:800737/1‑251 (MQ=255)                                                                                                                                                                      acaTCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAATCTCAA‑TAGAAACCCCCTTTGGCGGCGGG‑CGATATG‑CGGGATGCGGTTTCAGACGGGCaattac                                                                         >  1:691599/1‑246 (MQ=255)                                                                                                                                                                       caTCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAATATCAA‑TAGAAACCCACTTGAGCGGCGGA‑CGATAGT‑CGGGATGCGGTTTCAGACGGGCATTAACg                                                                        <  1:943130/251‑1 (MQ=255)                                                                                                                                                                                    gTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGACTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAATATCAA‑TAGAAACCCCCTTGAGCGGCGGG‑CGATAGA‑CGGGGTGCGGGTTCAGACGGGCATTAACGATAGCGCCATtgt                                                           >  1:265431/1‑251 (MQ=255)                                                                                                                                                                                      tGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAATATCAA‑TAGAAACCCACTTGAGCGGCGGA‑CGATAGT‑CGGGATGCGGTTTCAGACGGGCATTAACGATAGCGCCATtgtg                                                          <  1:515522/250‑1 (MQ=255)                                                                                                                                                                                       ggCAAACCAGGCGTTGAGTTTTGCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAATATCAA‑TAGAAACCCACTTGAGCGGCGGA‑CGATAGT‑CGGGATGCGGTTTCAGACGGGCATTAACGATAGCGCCATTGTGCa                                                        <  1:581515/251‑1 (MQ=255)                                                                                                                                                                                           aaCCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAATATCAA‑TAGAAACCCACTTGAGCGGCGGA‑CGATAGT‑CGGGATGCGGTTTCAGACGGGCATTAACGATAGCGCCATTGTGCA‑TATc                                                   <  1:153279/251‑1 (MQ=255)                                                                                                                                                                                           aaCCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAATATCAA‑TAGAAACCCACTTGAGCGGCGGA‑CGATAGT‑CGGGATGCGGTTTCAGACGGGCATTAACGATAGCGCCATTGTGCA‑TATc                                                   <  1:48046/251‑1 (MQ=255)                                                                                                                                                                                            accaggatttGATTTTTTCCAGTAGCTGCGGGTGGCTGGCGACGTATTCCCGTTCAAAATCGAGCGCGTCGGCCTCGCCATTCTCTGGTCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCTCCGTGGCGGGTGGTTTCAATATCAA‑TAGAAACCCCCTTGAGCGGCGGA‑CGATAGT‑CGGGTTGCGGTTTCAGACGGGCATTAACGATAGCGCCATTGTGCA‑TATCa                                                  <  1:720123/243‑1 (MQ=255)                                                                                                                                                                                              cAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAATATCAA‑TAGaaa                                                                                                                               <  1:522395/175‑1 (MQ=255)                                                                                                                                                                                                   gTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAATATCAA‑TAGAAACCCACTTGAGCGGCGGA‑CGATAGT‑CGGGATGCGGTTTCAGACGGGCATTAACGATAGCGCCATTGTGCA‑TATCACCCTCGa                                           <  1:227137/251‑1 (MQ=255)                                                                                                                                                                                                     tGAGTTTTTCCAGTAGTGGCCGGCGGTTGGCGACGTATGCCAGTTAAAAATCGAGCGCGGAGGCGTCGCAATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTGCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAATAGCAA‑TAGAAACCCACTTGAGCGGCTGA‑CGATAGT‑CGGGATGCGGTTTCAGACGGTCATTAACGATAGCGCCATTGTGCA‑TATCACCCTCGAc                                          <  2:327960/250‑1 (MQ=255)                                                                                                                                                                                                      gaatttttCCAGGAGCGGCGGGCGGGTGGCGACGTATTCCAGTGCAAAACGTAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAATATCAA‑TAGAAACCCACTTGAGCGGCGGA‑CGATAGT‑CGGGATGCGGTTTCAGACGGGCATTAACGATAGCGCTATTGTGCA‑TATCACCCTCGAccc                                        <  2:120250/248‑1 (MQ=255)                                                                                                                                                                                                      gAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAATATCAA‑TAAAAACCCCATTGAGCGGCGGG‑CGATAGT‑CGGGGTGCGGTTTCAGACGGGCATTAACGATAGCGCCATTGTGCA‑TATCACCCTCGAccc                                        >  1:983954/1‑251 (MQ=255)                                                                                                                                                                                                       aGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCTTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAATATCAA‑TAGAAACCCACTTGAGCGGCGGA‑CGATAGT‑CGGGATGCGGTTTCAGACGGGCATTAACGATAGCGCCATTGTGCA‑TATCACCCTCGACCca                                       <  1:747526/251‑1 (MQ=255)                                                                                                                                                                                                             tccagtagcTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAATATCAA‑TAGAAACCCACTTGAGCGGCGGA‑CGATAGT‑CGGGATGCGGTTTCAGACGGGCATTAACGATAGCGCCATTGTGCA‑TATCACCCTCGACCCACACCg                                  <  1:324011/242‑1 (MQ=255)                                                                                                                                                                                                               cagtagcTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAATATCAA‑TAGAAACCCACTTGAGCGGCGGA‑CGATAGT‑CGGGATGCGGTTTCAGACGGGCATTAACGATAGCGCCATTGTGCA‑TATCACCCTCGACCCACACCggtg                               <  1:919965/245‑1 (MQ=255)                                                                                                                                                                                                                   agcTGCGGTCGGCTGGCGACGTATTCCATTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAATATCAA‑TAGAAACCCACTTGAGCGGCGGA‑CGATAGT‑CGGGATGCGGTTTCAGACGGGCATTAACGATAGCGCCATTGTGCA‑TATCACCCTCGACCCACACCggtgagg                            <  2:246798/248‑1 (MQ=255)                                                                                                                                                                                                                    gcTGCGGGCGGCTGGCGACGTATTCAAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGGGGGTTCCATATCAA‑AAGAAACCCCCTTTAGCGGCGGG‑CGATAGTTGGGGAGGGGGTTT‑AGACCGGCGTTAACACTAGCGCCATGTTGCA‑TATCACCCCCCACCCACCCCggggggggg                          >  2:65218/2‑244 (MQ=255)                                                                                                                                                                                                                         gggcggCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAGCGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAATATCAA‑TAGAAACCCACTTGAGCGGCGGA‑CGATAGT‑CGGGATGCGGTTTCAGACGGGCATTAACGATAGCGCCATTGTGCA‑TATCACCCTCGACCCACACCGGTGAGGTGATaaa                     <  1:727347/251‑1 (MQ=255)                                                                                                                                                                                                                         gggcggCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCCATATCAA‑TAGAAACCCACTTGAGCGGCGGA‑CGATAGT‑CGGGATGCGGGTTCAGACGGGCATTAACGCAAGAGCCCTTGTGCG‑TATCACCCCCGACCCACACCggtgaggggctaa                      >  1:91370/1‑244 (MQ=255)                                                                                                                                                                                                                         gggcggCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAATATCAA‑TAGAAACCCACTTGAGCGGCGGA‑CGATAGT‑CGGGATGCGGTTTCAGACGGGCATTAACGATAGCGCCATTGTGCA‑TATCACCCTCGACCCACACCGGTGAGGTGATaaa                     <  1:672799/251‑1 (MQ=255)                                                                                                                                                                                                                             ggcTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGGGGTTTCAATATCAA‑TAGAAACCCACTTGAGCGGCGGA‑CGATAGT‑CGGGATGCGGTTTCAGACGGGCATTAACGATAGCGCCATTGTGCA‑TATCACCCTCGACCCACCCCGGTGGGGTGATAAACCGc                 >  1:258590/1‑251 (MQ=255)                                                                                                                                                                                                                              gcTGGCGACGTATTCCAGTTCAAAATCGATCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGATTCAATATCAA‑AAGAAACCCACTTGAGGGGCGGAAGGATA‑T‑CCGGGTGGGGTTTTAGACGGGGATTAACGAAAGCGCCATTTTGAAACACCACC‑TCGATCCCCACCGGGGAGGGGATAAACCGcc                >  1:162664/1‑250 (MQ=255)                                                                                                                                                                                                                                 ggCGACGTATTCCAGTTCAAAAGCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCGCACCGTGGCGGGTGGTTTCAATAGCAA‑TAGAAACCCACTTGAGCGGCGGA‑CGATAGT‑CGGGATGCGGTTTCAGACGGGCATTAACGATAGCGCCATTGTGCA‑TATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCTc               <  2:293319/249‑1 (MQ=255)                                                                                                                                                                                                                                  gCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAATATCAA‑TAGAAACCCACTTGAGCGGCGGA‑CGATAGT‑CGGGATGCGGTTTCAGACGGGCATTAACGATAGCGCCATTGTGCA‑TATCAACCTCGACCCACACCGGTGAGGTGATAAACCGCTCCAt            <  1:229483/251‑1 (MQ=255)                                                                                                                                                                                                                                         aTTCCAGTTCAAAACCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAATATCAA‑TAGAAACCCACTTGAGCGGCGGA‑CGATAGT‑CGGGATGCGGTTTCAGACGGGCATTAACGATAGCGCCATTGTGCA‑TATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCTCCATCAGATAg     <  2:268797/251‑1 (MQ=255)                                                                                                                                                                                                                                            ccAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAATATCAA‑TAGAAACCCACTTGAGCGGCGGA‑CGATAGT‑CGGGATGCGGTTTCAGACGGGCATTAACGATAGCGCCATTGTGCA‑TATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCTCCATCAGATAGCg   <  1:128705/250‑1 (MQ=255)                                                                                                                                                                                                                                            ccAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAATATCAA‑TAGAAACCCACTTGAGCGGCGGA‑CGATAGT‑CGGGATGCGGTTTCAGACGGGCATTAACGATAGCGCCATTGTGCA‑TATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCTCCATCAGATAGCGt  <  1:194925/251‑1 (MQ=255)                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                  AGCCGACCTTTAGCCTGGGCAAAAAA‑GACG‑CCGTTTTTAAAGCCGTGCTCGCGCC‑ACTCCAGCTCGCTATTATCGCGCCCAAGACGCAGCGGAAGACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGCAACTGCGGGCGGCTGGCGACGTATTCCAGTTCGAAATCAAGCGAGGAGGCGTCGCCATTCTCCGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAATATCTA‑TAGAAACCCACTTGAGCGGCGGA‑CGATAGT‑CGGGATGCGGTTTCAGACGGGCATTAACGATAGTGCCATTGTGCA‑TATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCTCCATCAGATAGCGT  >  NC_000913/64963‑65445

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑