BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	82,933	G→A	I199I (ATC→ATT)	leuA ←	2‑isopropylmalate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	82,933	0	G	A	100.0%	350.5 / NA	113	I199I (ATC→ATT)	leuA	2‑isopropylmalate synthase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (52/61); total (52/61)

Only 100 of 113 total aligned reads displayed.

GATCGGCATATTACAAATCTGGCTAACTAACTGGCTGGTGCGCCATATCTCCTGGTGATTAATGGCGGTGTGGACGTTGAGAATATCCTTACGAACT‑TTGATCGCC‑ATGATGACTTCTTCCAGGGAACAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCCAGTGAGTTTCCGACCGCCAGGCC‑CAAATCGTCGTGGGTATGTACGGAGATAATGGCTTTGTCGATGTTAGGCACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACT‑CAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATCGGCAATGGGTGTACGCCCGGCATCTTCGCAAGAAAATTCAACATCATCGGTGTAATTACGGGCGCGTTTCACCATATAGATAGCGCGTTCGATCAC  >  NC_000913/82687‑83178

gaccGGCATATTACAAATCTGGCTAACTAACTGGCTGGTGCGCCATATCTCCTGGTGATTAATGGCGGTGTGGACGTTGAGAATATCCTTACGAACT‑TTGATCGCC‑ATGATGACTGCTGCCAGGGAACAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCCAGTGAGTTGCCGACCGCCAGGCC‑TAAATCGTCGTGGGTATGTACGGAAATaa                                                                                                                                                                                                                                                    <  2:907294/248‑1 (MQ=255)

     gCATATTACAAATCTGGCTAACTAACTGGCTGGTGCGCCATAGCTCCTGGTGATTAATGGCGGTGTGGACGTTGAGAATATCCTTACGAACT‑TTGATCGCC‑ATGATGACTTCTTCCAGGGAACAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCCAGTGAGTTGCCGACCGCCAGGCC‑TAAATCGTCGTGGGTATGTACGGAAATAATGGCt                                                                                                                                                                                                                                               <  2:295783/251‑1 (MQ=255)

      cATATTACAAATCTGGCTAACTAACTGTCTGGTGCGCCATATCTCCTGGGGATTAATGGCGGTGTGGACGTTGAAAATATCCTTACGAACT‑TTGATCGCC‑ATGATGACTTCTTCCAGGGAACAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCCCCTGGCGTGCACCGGCATGTACCGCCGCCAGTGAGTTGCCGACCGCCAGGCC‑TAAATCGTCGTGGGTATGTACGGAAATAATGGCtt                                                                                                                                                                                                                                              <  2:961729/251‑1 (MQ=255)

       atatTACAAATCTGGCTAACTAACTGGCTGGTGCGCCATATCTCCTGGTGATTAATGGCGGTGTGGACGTTGAGAATATCCTTACGAACT‑TTGATCGCC‑ATGATGACTTCTTCCAGGGAACAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCCAGTGAGTTGCCGACCGCCAGGCC‑TAAATCGTCGTGGGTATGTACGGAAATAATGGCttt                                                                                                                                                                                                                                             <  1:5536/251‑1 (MQ=255)

       atatTACAAATCTGGCTAACTAACTGGCTGGTGCGCCATATCTCCTGGTGATTAATGGCGGTGTGGACGTTGAGAATATCCTTACGAACT‑TTGATCGCC‑ATGATGACTTCTTCCAGGGAACAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCCAGTGAGTTGCCGACCGCCAGGCC‑TAAATCGTCGTGGGTATGTACGGAAATAATGGCttt                                                                                                                                                                                                                                             <  1:978678/251‑1 (MQ=255)

          ttACAAATCTGGCTAACTAACTGGCTGGTGCGACATATCTCCTGGTGATTAATGGCGGTGTGGACGTTGAGAAAATCCTTACGAAAT‑TTGATCGCC‑ATGATGACTTCTTCCAGGGGACAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTTCCGCCGCCCGTGTGTTACCGCCCGCCAGGCC‑CAAATCGTCCTGGGTATGTACGGGAATAATGGCTTTGTc                                                                                                                                                                                                                                          >  1:822092/1‑251 (MQ=255)

             aaaaTCTGGCTAACTAACGGGCTGGTGCGCCATATCTCCTGGTGATTAATGGCGGTGTGGACGTTGAGAATATCCTTACGAACT‑TTGATCGCC‑ATGATGACTTCTTCCAGGGAACAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCCAGTGAGTTGCCGACCGCCAGGCC‑TAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGAt                                                                                                                                                                                                                                       <  1:368988/250‑1 (MQ=255)

                 cctgtctaacaaaCTGGCTGGTGCGCCATATCTCCGGGTGATTAATGGCTGTGTGGACGGTGAGAATACCCTTACCAACT‑TTTAGCGCC‑ACGATGACTTCTTCCAGGGAACAGTTTCCGGCACCCTCGCCGACCCCGTTCATTGCGCCTTCCACCTGGCGTGCTCCGACATGTACCGCCGCCAGTGAGTGGCCGACCGCCAGGCC‑TAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTa                                                                                                                                                                                                                                   <  2:509456/246‑1 (MQ=255)

                    ggCTAACTAACTGGCTGGTGCGCCATATCTCCTGGTGATTAATGGCGGTGTGGACGTTGAGAATATCCTTACGAACT‑TTGATCGCC‑ATGATGACTTCTTCCAGGGAACAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCCAGTGAGTTGCCGACCGCCAGGCC‑TAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAgg                                                                                                                                                                                                                                 <  1:620533/250‑1 (MQ=255)

                            aactGGCTGGTGCGCCATATCTCCTGGTGATTAATGGCGGTGTGGGCGTTGAGAATATCCTTACGAACT‑TTTATCGCC‑ATGATGACTTCTTCCAGGGAACAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCCAAGGAGGTGCCGACCGCCAGGCCTCAAA‑CGTCGGGGGTATGGAAGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTc                                                                                                                                                                                                                        >  1:389803/1‑251 (MQ=255)

                            aacgggctggTGCGCCATATCTCCTGGGGATTAATGGCGGTGTGGACGTTGAGAATATCCTTACGAACT‑TTGATCGCC‑ATGATGACTTCTTCCAGGGAACAGTTTCCGGCACGCTCGCCGATCCCGTGCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCCAGTGAGTTGCCGACCGCCAGGCC‑TAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGt                                                                                                                                                                                                                          <  1:651285/245‑1 (MQ=255)

                             actGGCTGGTGCGCCATATCTCCTGGTGATTAATGGCGGTGTGGACGTTGAGAATATCCTTACGAACT‑TTGATCGCC‑ATGATGACTTCTTCCAGGGAACAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCCAGTGAGTTGCCGACCGCCAGGCC‑TAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCa                                                                                                                                                                                                                       <  1:241646/251‑1 (MQ=255)

                                ggctggTGCGCCATATCTCCTGGTGATTAATGGCGGTGTGGACGTTGAGAATATCCTTACGAACT‑TTGATCGCC‑ATGATGACTTCTTCCAGGGAACAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCCAGTGAGTTGCCGACCGCCAGGCC‑TAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGttaat                                                                                                                                                                                                                      <  1:610529/249‑4 (MQ=255)

                                    ggTGCGCCATATCTCCTGGTGATTAATGGCGGTGTGGGAGTTGAGAAAATCCTTACGAACT‑TTTATCGCC‑ATGATGACTTCTTCCAGGGAACAATTTTCGGCACGCTCGCCGATCCCCTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCCCGAGGGGTGCCGACCGCCAAGCC‑TCAATCGTCGTGGGTATGGACGGGAATAATGGCTTTGTCGATGTTTGGGACGCGTTCCTAccggg                                                                                                                                                                                                                >  1:220219/1‑247 (MQ=255)

                                    ggTGCGCCATATCTCCTGGTGATTAATGGCGGTGTGGACGTTGAGAATATCCTTACGAACT‑TTGATCGCC‑ATGATGACTTCTTCCAGGGAACAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCCAGTGAGTTGCCGACCGCCAGGCC‑TAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAgg                                                                                                                                                                                                                 <  1:101211/250‑1 (MQ=255)

                                        cgcCATATCTCCTGGTGATTAATGGCGGTGTGGACGTTGAGAATATCCTTACGAACT‑TTGATCGCC‑ATGATGCCTTCTTCCAGGGAACAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCCAGTGAGTTGCCGACCGCCAGGCC‑TAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCt                                                                                                                                                                                                            <  1:342100/251‑1 (MQ=255)

                                            atatCTCCTGGTGATTAATGGCGGTGTGGACGTTGAGAATATCCTTACGAACT‑TTGATCGCC‑CTGATGACTTCTTCCAGGGAACAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCCCGTGAGTTGCCGACCCCCAGGCC‑TAAATCGTCGTGGGGATTTACGGGAATAATGGCTTTGTCGATGTTAGGGACGGGTTCATACAGGCCGCtggtg                                                                                                                                                                                                        >  1:874006/1‑248 (MQ=255)

                                            atatCTCCTGGTGATTAATGGCGGTGTGGACGGTGAGAATATCCTTACGAACT‑TTGATCGCG‑ATGTTGACTTCTTCCAGGGAACAGTGGCCGGCACGCTCGCCGATCCCGTTCATTGCGCTTTCTACCGGGCGTGCACCGGCAGGTACCGCCGCCAGTGAGTTGCGGACCGCCAGGCC‑TAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCtgatg                                                                                                                                                                                                        <  1:129762/251‑1 (MQ=255)

                                                               ggCGGTGTGGACGTTGAGAATATCCTTACGAACT‑TTGATCGCC‑ATGATGACTTCTTCCAGGGAACAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCCAGTGAGTTGCCGTCCGCCAGGCC‑TAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACT‑CAAAc                                                                                                                                                                                     <  1:580280/250‑1 (MQ=255)

                                                                gCGGTGTGGACGTTGAGAATATCCTTACGAACT‑TTGATCGCC‑ATGATGACTTCTTCCAGGGAACAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCCCCGGCATGTACCGCCGCCAGTGAGTTGCCGACCGCCAGGCC‑TAAAGCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACT‑CAAACgg                                                                                                                                                                                   <  1:165691/251‑1 (MQ=255)

                                                                gCGGTGTGGACGTTGAGAATATCCTTACGAACT‑TTGATCGCC‑ATGATGACTTCTTCCAGGGAACAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCCAGTGAGTTGCCGACCGCCAGGCC‑TAAATCGTCGTGGGGATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATTAAGGCCGCTGATGATTCCGGCGAACT‑CAAACgg                                                                                                                                                                                   >  2:207263/1‑251 (MQ=255)

                                                                   gtgtgtACGTTGAGAATATCCTTACGAACT‑TTGATCGCC‑ATGATGACTTCTTCCAGGGAACAGTTTCCGGCCCGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCCAGTGAGTTGCCGACCGCCAGGAC‑ACACTCGTCGTGGGTATGTAAGGAAATAATGGCTTTGACGTTGTTAGGGACGCGTTCATACGTGCCGCTTATGGTTACGGCGGACTACAAACgcc                                                                                                                                                                                  >  2:638138/1‑248 (MQ=255)

                                                                    tgtgGACGTTGAGAATATCCTTACGAACT‑TTGATCGCC‑ATGATGACTTCTTCCAGGGAACAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCCAGTGAGTTGCCGACCGCCAGGCC‑TAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGGTGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACT‑CAAACGGCATg                                                                                                                                                                               >  2:213728/1‑251 (MQ=255)

                                                                      tgGACGTTGAGAATATCCTTACGAACT‑TTGATCGCC‑ATGATGACTTCTTCCAGGGAACAGTTTCCGGCACGCTCGCCGATCCCGTTCCTTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCCAGTGAGTTGCCGACCGCCAGGCC‑TAAATCGGCGTGGGTATGTACGGAAATCATGGCTTTGTCGATGTTAAGGACGCGTTCATACAGGCCGCTGCAGATGCCGGCGAACT‑CAAACGGCCTGgt                                                                                                                                                                             >  2:382669/1‑251 (MQ=255)

                                                                          cGTTAAGAATATCCTTACGACCC‑TTGATCGCC‑ATGATGACTTCTTCCAGGGAACAGTTTCCGGACCGCTCGCCGATCCCGTACATGGCGCCTGCCACCTGGCGTGCCCCGGCATGTCCCGCCGCCAGTGAGTTGCCGACCGCCAGGCC‑TAAAACGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTGCGCGTTCATACAGGCCGCTGATGATTCCGGCGAACT‑CAAACGGCATGGTGTAg                                                                                                                                                                         <  1:581368/251‑1 (MQ=255)

                                                                             gtaaaaTATCCTGACGA‑CTGTTGATCGCCTAT‑ATGCCTTCTTCCGGGTAACAGTTTCCGGCCCGCCCTCCGAGCCAGTCCATGTCGCCTTCCCCCTGGTGTGCCCCGTCATGTACCGCCGCCAGTGAGTTGCCGACCGCCAGGCC‑TAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACT‑CAAACGGCATGGTGTAGccc                                                                                                                                                                      <  2:505209/247‑1 (MQ=255)

                                                                              gagaATATCCTTACGAACT‑TTGATCGCC‑ATGATGACTTCTTCCAGGGAACAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCCAGTGAGTTGCCGACCGCCAGGCC‑TAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACT‑CAAACGGCATGGTGTAGccc                                                                                                                                                                      <  1:567961/250‑1 (MQ=255)

                                                                               agaATATCCTTACGAACT‑TTGATCGCC‑ATGATGACTTCTTCCAGGGAACAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCCAGTGAGTTGCCGACCGCCAGGCC‑TAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACT‑CAAACGGCATGGTGTAGCCCAc                                                                                                                                                                    <  1:129838/251‑1 (MQ=255)

                                                                               agaATATCCTTACGAACT‑TTGATCGCC‑ATGATGACTTCTTCCAGGGAACAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCCAGTGAGTTGCCGACCGCCAGGCC‑TAAATCGTCGTGGGTATGTAAGGAAATAATGGCTTTGTCGCTGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACG‑CAACCGGCCTGgtgtgaccccc                                                                                                                                                                    >  2:695316/1‑244 (MQ=255)

                                                                                      ccTTACGAACT‑TTGATCGCC‑ATGATGACTTCTTCCAGGGAACAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCCAGTGAGTTGCCGACCGCCAGGCC‑TAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACT‑CAAACGGCATGGTGTAGCCCACGGTGTc                                                                                                                                                              <  1:888318/250‑1 (MQ=255)

                                                                                      ccTTACGAACT‑TTAATCCCC‑AGAATTCCTTCTCCCAGGGACCAGTTTCCGGCCCGCTCGCCTATCCCGTTCATTGCGCCTTCCCCCTGGCGTGCCCCGGCATGTCCCGCCGCCAGTGAGTTGCCGACCGCCAGGCC‑TAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACT‑CAAACGGCATGGTGTAGCCCACGGTGTcc                                                                                                                                                             <  2:589110/251‑1 (MQ=255)

                                                                                       cTTACGAACT‑TTGATCGCC‑ATGATGACTTCTTCCAGGGAACAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCCAGTGAGTTGCCGACCGCCAGGCC‑TAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGATCATACAGGCCGCTGATGATTCCGGCGAACT‑CAAACGGCATGGTGTAGCCCACGGGGTCCg                                                                                                                                                            >  1:109664/1‑251 (MQ=255)

                                                                                            aaaCT‑TTGACCGCC‑ATGATTACTTCTTCCAGTGAACATTTTCCGGCACGCCCCCCGATCCCGTTCATTGCGCCGTCCACCTGGCGTGCACCGGCATGTACCGCCGCCAGTGAGTTGCCGACCGCCAGGCC‑TAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACT‑CAAACGGCATGGTGTAGCCCACGGTGTCCGGAATg                                                                                                                                                       <  1:65622/250‑1 (MQ=255)

                                                                                               cT‑TTGATCGCC‑ATGATGACTTCTTCCAGGGAACAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCCAGTGAGTTGCCGACCGCCAGGCC‑TAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACT‑CAAACGGCATGGTGTAGCCCACGGTGTCCGGAttgttg                                                                                                                                                    >  1:638826/1‑251 (MQ=255)

                                                                                                t‑ttGATCGCC‑ATGATGACTTCTTCCAGGGAACAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCCAGTGAGTTGCCGACCGCCAGGCC‑TAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACT‑CAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTg                                                                                                                                                    >  1:113232/1‑250 (MQ=255)

                                                                                                   tttgcccccagggtgtcttcttCCAGGGACCATTTTCCGGCACGCTCGCCGACCCCGTTCATTGCGCCTTCCACCTGGCGGGCACCGGCATGTACCGCCGCCAGTGAGTTGCCGACCGCCAGGCC‑TAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACT‑CAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGa                                                                                                                                                   <  2:247489/234‑1 (MQ=255)

                                                                                                   tGATCGCC‑ATGATGACTTCTTCCAGGGAACAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCCAGTGAGTTGCCGACCGCCAGGCC‑TAAATCGTCGTGGGTATGCACGGAAATAAAGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGACCT‑CAAACGGCATGGTATAGCCCAAGGCGTCCGGAATGTTgttg                                                                                                                                                 >  2:424175/1‑248 (MQ=255)

                                                                                                    gaccGCC‑ATGATGCCTTCTTCCATGGAACAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCGCCTTGCTTGCACCGGCATGTACCGCCGCCAGTGAGTTGCCGACCGCCAGGCC‑TAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGTCCGCTGATGATTCCGGCGAACT‑CAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGAtg                                                                                                                                                 <  1:965507/247‑1 (MQ=255)

                                                                                                     aTCGCC‑ATGATGACTTCTTCCAGGGAACAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCCAGTGAGTTGCCGACCGCCAGGCC‑TAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGGTGGTAGGTACGCGGTCATACAGGCCGCTGATGATTACGGCGAACT‑CAAACGGCATGGTGTAGACCACGGTGGCCGGGATGTTGAtggg                                                                                                                                               >  2:22213/1‑250 (MQ=255)

                                                                                                        gCC‑ATGATGACTTCTTCCAGGGAACAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCCAGTGAGTTGCCGACCGCCAGGCC‑TAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACT‑CAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGAtggtggg                                                                                                                                            >  1:134568/1‑250 (MQ=255)

                                                                                                        ccc‑ATGATGACTTCTTCCAGGGAACAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCCAGTGAGTTGCCGACCGCCAGGCC‑TAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACT‑CAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGAtggtggt                                                                                                                                            <  1:835937/250‑1 (MQ=255)

                                                                                                          ccatgtgtgcttctcCCAGGGAACAGTTTGCGGCACGCGCGCCGACCCCGTTCATTTCGCCTTCCACCGGGCTAGCATCGGCATGTACCGCCGCCAGTGAGTTGCCGACCGCCAGGCC‑TAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACT‑CAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGAtggtggtg                                                                                                                                           <  2:112423/242‑1 (MQ=255)

                                                                                                          c‑ATGATGACTTCTTCCAGGGAACAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCCAGTGAGTTGCCGACCGCCAGGCC‑TAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACT‑CAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGAtggtggtgg                                                                                                                                          <  1:470834/251‑1 (MQ=255)

                                                                                                               atgaCTTCTTCCAGGGAAAAGTTTCCGGCACGCTCGCCGCTCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCCAGTGAGTTGCCGCCCGCCAGGCC‑GAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGGTCATACAGGCCGCTGATGATTCCGGCGAACT‑CAACCAGCATGGTGTAGACCAAGGTGTCCGGAATGTTGATGGGGGTGGCAcg                                                                                                                                      >  2:782570/1‑250 (MQ=255)

                                                                                                                  aCTTCTTCCAGGGAACAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCAGCCCGTGAGTTGCCGACCGCCAGGCC‑TAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTATCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACT‑AAAACGGAATGGTGTAGCCCACGGAGTCCGGAATGTTGCTGGTGGTTGCACCGGc                                                                                                                                   >  1:900292/1‑251 (MQ=255)

                                                                                                                     tcttcCAGGGAACAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCCAGTGAGTTGCCGACCGCCAGGCC‑TAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACT‑CAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCAtt                                                                                                                                >  1:10766/1‑251 (MQ=255)

                                                                                                                      cttcCAGGGAACAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCCAGTGAGTTGCCGACCGCCAGGCC‑TAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACT‑CAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCAtt                                                                                                                                >  1:36874/1‑250 (MQ=255)

                                                                                                                                         ggCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCCAGTGAGTTGCCGACCGCCAGGCC‑TAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACT‑CAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGGGGTGGCACCGGCATTAATCGCCGCTTCGACCACTc                                                                                                            >  1:767566/1‑251 (MQ=255)

                                                                                                                                           cACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCCAGTGAGTTGCCGACCGCCAGGCC‑TAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACT‑CAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTcgc                                                                                                          >  1:304294/1‑251 (MQ=255)

                                                                                                                                            aCGCTCGCCGACCCCGTTCATTGCTCCTCCCACCTGGCGGGCTCCGGCATGTACCGCCTCCAGTGAGTTGCCGACCGCCAGGCC‑TAAAACGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACT‑CAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTcgcg                                                                                                         <  1:565446/251‑1 (MQ=255)

                                                                                                                                                 cGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCCAGTGAGTTGCCGACCGCCAGGCC‑TAAATCGTCGTGGGAATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACT‑CAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCgcgca                                                                                                       >  1:69832/1‑247 (MQ=255)

                                                                                                                                                  tccGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCCATTGAGTTGCCGACCGCCAGTCC‑TCAATACTCGTGGGTATGTACGGCAATAATGGCTTTGACGATGTTATGTACGCGTTCATACAGGCCGCTTATGATACCGGCGAACT‑CAAACGTCAGGGTGTAGCCACCGGTGTCAGGATTTTTAGTGGTGGAGGCACCGGCATTACTCGCATCTTCGACCCCTcgcgac                                                                                                       >  1:654259/2‑245 (MQ=255)

                                                                                                                                                  gCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCCAGTGAGTTGCCGACCGCCCGGCC‑TAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGTTGTTAGGTACGCGTTCCTACAGGCCGCTGATGATTCCGGCGAACT‑CAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATAGCCGCTTCGCCCACTcgcgaccgct                                                                                                   >  1:654250/1‑245 (MQ=255)

                                                                                                                                                   ccgttCCCGTTCATTGCGCCGTCCACCTGGCGGGCACCGGCATGTCCAGCCGCTAGTGAGTTGCCGACCGCCAGGCC‑TAAAGCGTCGTGGGTATGTACGGAAAGAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGTCGCTGATGATTCCGGCGAACT‑CAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGTCCACTCGCGACAGATc                                                                                                  <  1:547671/247‑1 (MQ=255)

                                                                                                                                                         ccGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCCAGTGAGTTGCCGACCGCCAGGCC‑TAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACT‑CAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATCGGCAAt                                                                                            <  1:202810/251‑1 (MQ=255)

                                                                                                                                                           gatCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCCAGTGCGTTGCCGACCGCCAGGCC‑TAAATCGTCGTGGGCATGTACGGAAATAATGACTTTGTCGCTGATAGGCACGCGTTCATACGGGCCGCTGCTGATACCGGCGAACT‑CAAACGAAATGGTGAAGCCCACGGTGTCCGGAATGTTGACGGTGGTGGCAACGGCATTACTCGCCGCTTCGACACCTCgcgcacgataggcaaagg                                                                                          >  1:897730/3‑237 (MQ=255)

                                                                                                                                                             tCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCCAGTGAGTTGCCGACCGCCAGGCC‑TAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACT‑CAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACCCGCGCCAGATCGGCAATgggg                                                                                        >  2:504106/1‑250 (MQ=255)

                                                                                                                                                                     ccTTCCACCTGGCGTGCACCGGCATGTACCGCCGCCAGTGAGTTGCCGACCGCCAGGCC‑TAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACT‑CAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATCGGCAATGGGTGTACGccc                                                                                <  1:700776/251‑1 (MQ=255)

                                                                                                                                                                     ccTTCCACCTGGCGTGCACCGGCATGTACCGCCGCCAGTGAGTTGCCGACCGCCAGGCC‑TAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACT‑CAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATCGGCAATGGGTGTACGccc                                                                                <  1:778961/251‑1 (MQ=255)

                                                                                                                                                                       cgccccctggcttcccccGGAATGTACCGCCGCCAAGGAGTTGCCGACCGCCAGGCC‑TAAACCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGGTCATACAGGCCGCTGATGATTCCGGCGAACT‑CAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATCGGCAATGGGTGTACGCCCg                                                                               <  2:295637/234‑1 (MQ=255)

                                                                                                                                                                        tCCACCTGGCGTGCACCGGCATGTACCGCCGCCAGTGAGTTGCCGACCGCCAGGCC‑TAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACT‑CAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATCGGCAATGGGTGTACGCCCg                                                                               <  1:299467/249‑1 (MQ=255)

                                                                                                                                                                         ccgccgggCGTGCACCGGCATGTACCGCCGCCAGTGAGTTGCCGACCGCCAGGCC‑TAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACT‑CAAACGGCATGGTGTAGCCCGCGGTGTCCGGAATGTTGATGGTGGTGGCACCTGCATTAATCGCCGCTTCGACCACGCGCGCCAGATCGGCAATGGGTGTACGCCCGGCa                                                                            <  1:565160/245‑1 (MQ=255)

                                                                                                                                                                          cACCTGGCGTGCACCGGCATGTACCGCCGCCAGTGAGTTGCCGACCGCCAGGCC‑TAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACT‑CAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATCGGCAATGGGTGTACGCCCGGCAt                                                                           >  1:365108/1‑251 (MQ=255)

                                                                                                                                                                            cagggCGTGAACCGTGATGTACCGTCGCCAGTGAGTTGCCGACCTCCAGGAC‑TAAATCGGCGTGGGTATGTACGGAAATAAGGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACT‑CAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATCGGCAATGGGTGTACGCCCGGCATCt                                                                         <  2:870563/248‑1 (MQ=255)

                                                                                                                                                                              gggCGTGCACCGTCTGGTACCGCCGCCAGTGGGTTGCCGACCGCCAGGCC‑TAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACT‑CAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATCGGCAATGGGTGTACGCCCGGCATCTTc                                                                       <  2:602309/250‑1 (MQ=255)

                                                                                                                                                                              cggCGTGCACCGGCATGTACCGCCGCCAGTGAGTTGCCGACCGCCAGGCC‑TAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACT‑CAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATCGGCAATGGGTGTACGCCCGGCATCTTc                                                                       <  2:233728/250‑1 (MQ=255)

                                                                                                                                                                                    gcatcgccATGTACCGCCGCCAGTGAGTGGCCGACCGCCAGGCC‑TAAATCGTCGTTGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACT‑CAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCTCTTCGACCACTCGCGCCAGATCGGCAATGGGTGTACGCCCGGCATCTTCGCAAg                                                                  <  2:789897/243‑1 (MQ=255)

                                                                                                                                                                                    gCACCGGCATGTACCGCCGCCAGTGAGTTGCCGACCGCCAGGCC‑TAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACT‑CAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGCCCACTCGCGCCAGTTCGGCAATGGGTGTACGCCCGGCATCTTCGCa                                                                    >  1:29740/1‑248 (MQ=255)

                                                                                                                                                                                     cACCGGCATGTACCGCCGCCAGTGAGTTGCCGACCGCCAGGCC‑TAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACT‑CAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATCGGCAATGGGTGTACGCCCGGCATCTTCGCAAGaa                                                                <  1:459528/251‑1 (MQ=255)

                                                                                                                                                                                      aCCGGCATGTACCGCCGCCAGTGAGATTCCGACCGACAGGCC‑TAAAACGTCGAGGGTATTTACGGAAATAATGGCTTTGTCGATGTTAGGGACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACA‑CAAACGGCATGGTGTAGCCCACGGTGACCGGAATGTTGATGGGGGTGGCACCGGCACTCATCGACGCTTCGACCACTCGCGCCAGATCCGCCATGGGTGAACACCCGGccacttcggaagga                                                                >  2:137760/1‑237 (MQ=255)

                                                                                                                                                                                       ccGGCATGTACCGCCGCCAGTGAGTTGCCGACCGCCAGGCC‑TAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACT‑CAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATCGGCAATGGGTGTACGCCCGGCATCTTCGCAAGaaa                                                               >  1:773805/1‑250 (MQ=255)

                                                                                                                                                                                          gcaggTACCGCCGCCAGTGAGTTGCCGACCGCCAGGCC‑TAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACT‑CAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATCGGCAATGGGTGTACGCCCGGCATCTTCGCAAGAAAATTc                                                           <  2:874006/247‑1 (MQ=255)

                                                                                                                                                                                           cATGTACCGCCGCCAGTGAGTTGCCGACCGCCAGGCC‑TAAATCGTCGTGGGTATGTAAGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACT‑CAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCCCTCGCGCCAGATCGGCAATGGGTGTACGCCCGGCATCTTCGAAAGACAATTc                                                           >  2:150686/1‑250 (MQ=255)

                                                                                                                                                                                               tACCGCCGCCAGTGGGTTGCCGACCGCCGGGCC‑TGAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACT‑CAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATCGGCAATGGGTGTACGCCCGGCATCTTCGCAAGAAAATTCAAcat                                                      >  1:223188/1‑251 (MQ=255)

                                                                                                                                                                                                   cccgccAGTGAGTTGCCGACCGCCAGGCC‑TAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACT‑CAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATCGGCAATGGGTGTACGCCCGGCATCTTCGCAAGAAAATTCAACatcatc                                                  <  2:226592/250‑1 (MQ=255)

                                                                                                                                                                                                     cgccAGTGAGTTGCCGACCGCCAGGCC‑TAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACT‑CCAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGGGGCCTCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATCGGCAATGGGTGTACGCCCGGCATCTTc                                                                       >  2:274495/1‑228 (MQ=255)

                                                                                                                                                                                                     cgccAGTGAGTTGCCGACCGCCAGGCC‑TAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACT‑CAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATCGGCAATGGGTGTACGCCCGGCATCTTc                                                                       <  1:274495/228‑1 (MQ=255)

                                                                                                                                                                                                       ccAGTGAGTTGCCGACCGCCAGGCC‑TAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACT‑CAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATCGGCAATGGGTGTACGCCCGGCATCTTCGCAAGAAAATTCAACATCATCGgtg                                              <  1:439255/251‑1 (MQ=255)

                                                                                                                                                                                                       ccAGTGAGTTGCCGACCGCCAGGCC‑TAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACT‑CAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATCGGCAATGGGGGTACGCCCGGCCTCTTCGCAAGAAAATTCAACTTCCTCGgtg                                              >  2:883312/1‑251 (MQ=255)

                                                                                                                                                                                                             aGTTGCCGACCGCCAGGCC‑TAAATCGTCGTGGGTATATACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACT‑CAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATCGGCAATGGGTGTACGCCCGGCATCTTCGCAAGAAAATTCAACATCATCGGTGTAATTa                                        <  1:936111/251‑1 (MQ=255)

                                                                                                                                                                                                                tgccGACCGCCAGGCC‑TAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACT‑CAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATCGGCAATGGGTGTACGCCCGGCATCTTCGCAAGAAAATTCAACATCATCGGTGTAATTACg                                      <  1:720139/248‑1 (MQ=255)

                                                                                                                                                                                                                 gccGACCGCCAGGCC‑TAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACT‑CAAACGGCATGGTGTAGCCCACGGTGTCCGGCATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATCGGCACTGGGTGTACGCCCGGCATCTTCGCAAGAAAATTCAACATCATCGGTGTAATTACggg                                    >  1:567853/2‑251 (MQ=255)

                                                                                                                                                                                                                 gccGACCGCCAGGCC‑TAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACT‑CAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATCGGCAATGGGTGTACGCCCGGCATCTTCGCAAGAAAATTCAACATCATCGGTGTAATTACgg                                     <  1:646896/249‑1 (MQ=255)

                                                                                                                                                                                                                  ccGACCGCCAGGCC‑TAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACT‑CAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATCGGCAATGGGTGTACGCCCGGCATCTTCGCAAGAAAATTCAACATCATCGGTGTAATTACGGgc                                   <  1:972162/251‑1 (MQ=255)

                                                                                                                                                                                                                  ccGACCGCCAGGCC‑TAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACT‑CAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATCGGCAATGGGTGTACGCCCGGCATCTTCGCAAGAAAATTCAACATCATCGGTGTAATTACGGgc                                   <  1:415575/251‑1 (MQ=255)

                                                                                                                                                                                                                        gCCAGGCC‑TAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACT‑CAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATCGGCAATGGGTGTACGCCCGGCATCTTCGCAAGAAAATTCAACATCATCGGTGTAATTACGGGCGCGttt                             >  1:242063/1‑251 (MQ=255)

                                                                                                                                                                                                                          cAGGCC‑TAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGGTGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACT‑CAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGGCCACTCGCGCCAGATCGGCAATGGGTGTACGCCCGGCATCTTCGCAAGAAAATTCAACATCATCGGTGTAATTACGGGCGCGTTTc                            >  1:394801/1‑250 (MQ=255)

                                                                                                                                                                                                                                  aaaTCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACT‑CAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATCGGCAATGGGTGTACGCCCGGCATCTTCGCAAGAAAATTCAACATCATCGGTGTAATTACGGGCGCGTTTCACCatat                     >  1:173404/1‑250 (MQ=255)

                                                                                                                                                                                                                                  aaaTCGTCGTGGGTATGTACGGAAATAATGGCTTTGGCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACT‑CAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATCGGCAATGGGTGTACGCCCGGCATCTTCGCAAGAAAATTCAACATCATCGGTGTAATTACGGGCGCGTTTCACCAtata                    <  1:165511/251‑1 (MQ=255)

                                                                                                                                                                                                                                   aaTCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACT‑CAAACGGCATGGAGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATCGGCAATGGGTGTACGCCCGGCATCTTCGCAAGAAAATTCAACATCATCGGTGTAATTACGGGCGCGTTTCACCatat                     >  1:467169/1‑249 (MQ=255)

                                                                                                                                                                                                                                    aTCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGCTTCCGGCGAACT‑CAAACGGCATGGTGTAGCCCACGGTGTCCGGCATGTTGATGGTGGTGGCACAGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATCGGCAATGGGTGTACGCCCGGCATCCTCGCAAGCAACTTCAACATCATCGGTGTAATTACGGGCGCGTTTCACCATatagc                  >  1:98447/1‑250 (MQ=255)

                                                                                                                                                                                                                                      cgtcgtGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACT‑CAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATCGGCAATGGGTGTACGCCCGGCATCTTCGCAAGAAAATTCAACATCATCGGTGTAATTACGGGCGCGTTTCACCATatagata                <  2:317109/251‑1 (MQ=255)

                                                                                                                                                                                                                                            gggTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACT‑CAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATCGGCAATGGGTGTACGCCCGGCATCTTCGCAAGAAAATTCAACATCATCGGTGTAATTACGGGCGCGTTTCACCATATAGATAGCGCGt          >  2:869539/1‑251 (MQ=255)

                                                                                                                                                                                                                                             ggTATGTACGGAAATAATGGCGTTGTCGATGTGAGGTACGCGTGCATACAGGCCGCTGATGATTCCGGCGAACT‑CAAACGGCATGGTGTAGCCCACGGTGTCCTGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATCGGCAATGGGTGTACGCCCGGCATCTTCGCAAGAAAATTCAACATCATCGGTGTAATTACGGGCGCGTTTCACCATATAGATAGCGCGtt         <  2:584551/251‑1 (MQ=255)

                                                                                                                                                                                                                                              gTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACT‑CAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATCGGCAATGGGTGTACGCCCGGCATCTTCGCAAGAAAATTCAACATCATCGGTGTAATTACGGGCGCGTTTCACCAAATAGATAGCGCGTTc        >  2:953831/1‑251 (MQ=255)

                                                                                                                                                                                                                                                aTGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACT‑CAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGCCCACTCGCGCCAGATCGGCAATGGGTGTACGCCCGGCATCTTCGCAAGAAAATTCAACATCATCGGTGTAATTACGGGCGCGTTTCACCATATAGATAGCGCGTTCg       >  2:757276/1‑250 (MQ=255)

                                                                                                                                                                                                                                                aTGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACT‑CAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATCGGCAATGGGTGTACGCCCGGCATCTTCGCAAGAAAATTCAACATCATCGGTGTAATTACGGGCGCGTTTCACCATATAGATAGCGCGTTCGa      <  1:870410/251‑1 (MQ=255)

                                                                                                                                                                                                                                                  gTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACT‑CAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCACTTCGACCACTCGCGCCAGATCGGCAATGGGTGTACGCCCGGCATCTTCGCAAGAAAATTCAACATCATCGGTGTAATTACGGGCGCGTTTCACCATATAGATAGCGCGTTCGAt     <  1:817817/250‑1 (MQ=255)

                                                                                                                                                                                                                                                  gTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACT‑CAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGAACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATCGGCAATGGGTGTACGCCCGGCATCTTCGCAAGAAAATTCAACATCATCGGTGTAATTACGGGCGCGTTTCACCATATAGATAGCGCGTTCGATc    >  1:129759/1‑251 (MQ=255)

                                                                                                                                                                                                                                                    aCGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACT‑CAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATCGGCAATGGGTGTACGCCCGGCATCTTCGCAAGAAAATTCAACATCATCGGTGTAATTACGGGCGCGTTTCACCATATAGATAGCGCGTTCGATCAc  >  1:82502/1‑251 (MQ=255)

GATCGGCATATTACAAATCTGGCTAACTAACTGGCTGGTGCGCCATATCTCCTGGTGATTAATGGCGGTGTGGACGTTGAGAATATCCTTACGAACT‑TTGATCGCC‑ATGATGACTTCTTCCAGGGAACAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCCAGTGAGTTTCCGACCGCCAGGCC‑CAAATCGTCGTGGGTATGTACGGAGATAATGGCTTTGTCGATGTTAGGCACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACT‑CAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATCGGCAATGGGTGTACGCCCGGCATCTTCGCAAGAAAATTCAACATCATCGGTGTAATTACGGGCGCGTTTCACCATATAGATAGCGCGTTCGATCAC  >  NC_000913/82687‑83178

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`