Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 82,909 C→T L207L (TTG→TTA)  leuA ← 2‑isopropylmalate synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091382,9090CT95.2% 331.5 / ‑4.9 107L207L (TTG→TTA) leuA2‑isopropylmalate synthase
Reads supporting (aligned to +/- strand):  ref base C (5/0);  new base T (40/60);  total (47/60)
Fisher's exact test for biased strand distribution p-value = 1.27e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 107 total aligned reads displayed.

ACAATGGCTTTGTTTGCCGGGATCGGCATATTACAAATCTGGCTAACTAACTGGCTGGTGCGCCATATCTCCTGGTGATTAATGGCGGTGTGGACGTTGAGAATATCCTTACGAACT‑TTGATCGCC‑ATGATGACTTCTTCCA‑GGGAACAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCC‑AGTGAGTTTCCGACCGCCAGGCC‑CAAATCGTCGTGGGTATGTACGGAGATAATGGCTTTGTCGATGTTAGGCACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACT‑CAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATCGGCAATGGGTGTACGCCCGGCATCTTCGCAAGAAAATTCAACATCATCGGTGTAATTACGGGCGCGTTTC  >  NC_000913/82667‑83152
                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                    
  aaTGGCTTTGTTTGCCGGGATCGGCATATTACAAATCTGGCTAACTAACTGGCTGGTGCGCCATATCTCCTGGTGATTAATGGCGGTGTGGACGTTGGGAATATCCTTTCGAACT‑TTTATCGCC‑CTGATGACTTCTTTCC‑GGGAACAGTTTTCGGCCAGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCCTGTACCCCCGCCCAG‑GAGTTGCCGACCGCCAGGCC‑CAAAtactcgg                                                                                                                                                                                                                                            >  1:779105/1‑245 (MQ=255)
    tGGCTTTGTTTGCCGGGATCGGCATATTACAAATCTGGCTAACTAACTGGCTGGTGCGCCATATCTCCTGGTGATTAATGGCGGTGTGGGAGTTGAGAATATACTTACAAACT‑TTTATAGCC‑CTGATGACTTTTTCCC‑GGGAAAAGTTTTCGGCACGCTCTCCCAGCCCGTTCATTGCGCCTTCCCCCTGGCGGGCACCGGCATGGTCCGCCGCC‑AGTGAGTTGGCGACCCCCAGGCC‑CAAAacgtggggg                                                                                                                                                                                                                                          >  2:674821/1‑246 (MQ=255)
          tgtttgCCGGGATCGGCATATTACAAATCTGGCTAACTAACTGGCTGGTGCGCCATATCTCCTGGTGATTAATGGCGGTGGGGACGTTGAGAATATCCTTACGAACT‑TTGGTCGCC‑ATGATGACTTCTTCCAAGGGAA‑AGATTCCGGCACGCTCGCCGATACCGTTCATTGCGCCTTCCACCCGGCGTGCACCGGGATGTACCGCCGCC‑CGTGGGTTTCCGCCCGCCCAGAC‑CAAATCGACGTgggggtgt                                                                                                                                                                                                                                    >  1:259284/1‑246 (MQ=255)
              ggCCGGGATCGGCATATTACAAAGCTGGCTAACTAACTGGCTGGTGCGCCATATCTCCTGTTGATTAATGGCGGTGTGGACGTTGAGAATATCCTTACGAACT‑GTGATCGCC‑ATGATGACTTCTTCCA‑GGGAACAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCC‑AGTGAGTTGCCGACCGCCAGGCC‑TAAATCGTCGTGGGTATGTACgg                                                                                                                                                                                                                                <  2:598816/250‑1 (MQ=255)
                ccGGGATCGGCATATTACAAATCTGGCTAACTAACTGGCTGGTGCGCCATATCTCCTGGTGATTAATGGCGGTGTGGACGTTGAGAATATCCTTACGAACT‑TTGATCGCC‑ATGATGACTTCTTCCA‑GGGAACAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCC‑AGTGAGTTGCCGACCGCCAGGCC‑TAAATCGTCGTGGGTATGTACGgaa                                                                                                                                                                                                                              <  1:545488/251‑2 (MQ=255)
                  gggATCGGCTTATTACAAATCTGGCTAACTAACGGGCTGGGGCGCCATATTCCCTGGGGATTAAGGGCGGTGTGGACGTTGAGAATATCCTGACGAACT‑TTGATCGCC‑ATGATTACTTCTTCCA‑GGGAACAGTTTCCGGCACGGTCGCCGATCCCGTTCATTGCGCCTTCCTCCTGTCGTGCACCGGCTTGTACCGCCGCC‑AGTGAGTTGCCGACCGCCAGGCC‑TAAATCGTCGTGGGTATGTACGgaaat                                                                                                                                                                                                                            <  1:114215/251‑4 (MQ=255)
                    gaccGGCATATTACAAATCTGGCTAACTAACTGGCTGGTGCGCCATATCTCCTGGTGATTAATGGCGGTGTGGACGTTGAGAATATCCTTACGAACT‑TTGATCGCC‑ATGATGACTGCTGCCA‑GGGAACAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCC‑AGTGAGTTGCCGACCGCCAGGCC‑TAAATCGTCGTGGGTATGTACGGAAATaa                                                                                                                                                                                                                          <  2:907294/248‑1 (MQ=255)
                    gATCGTCATATTACAAATCTGGCTAACTAACTGGCTGGTGCGCCATATCTCCTGGTGATTAATGGCGGTGTGGACGTTGAGAATATCCTTACGAACT‑TTGATCGCC‑ATGATGACTTCTTCCA‑GGGAACAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCC‑AGTGAGTTGCCGACCGCCAGGCC‑TAAATCGTCGTGGGTATGTACGGAAATaa                                                                                                                                                                                                                          <  1:928958/251‑1 (MQ=255)
                         gCATATTACAAATCTGGCTAACTAACTGGCTGGTGCGCCATAGCTCCTGGTGATTAATGGCGGTGTGGACGTTGAGAATATCCTTACGAACT‑TTGATCGCC‑ATGATGACTTCTTCCA‑GGGAACAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCC‑AGTGAGTTGCCGACCGCCAGGCC‑TAAATCGTCGTGGGTATGTACGGAAATAATGGCt                                                                                                                                                                                                                     <  2:295783/251‑1 (MQ=255)
                          cATATTACAAATCTGGCTAACTAACTGTCTGGTGCGCCATATCTCCTGGGGATTAATGGCGGTGTGGACGTTGAAAATATCCTTACGAACT‑TTGATCGCC‑ATGATGACTTCTTCCA‑GGGAACAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCCCCTGGCGTGCACCGGCATGTACCGCCGCC‑AGTGAGTTGCCGACCGCCAGGCC‑TAAATCGTCGTGGGTATGTACGGAAATAATGGCtt                                                                                                                                                                                                                    <  2:961729/251‑1 (MQ=255)
                           atatTACAAATCTGGCTAACTAACTGGCTGGTGCGCCATATCTCCTGGTGATTAATGGCGGTGTGGACGTTGAGAATATCCTTACGAACT‑TTGATCGCC‑ATGATGACTTCTTCCA‑GGGAACAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCC‑AGTGAGTTGCCGACCGCCAGGCC‑TAAATCGTCGTGGGTATGTACGGAAATAATGGCttt                                                                                                                                                                                                                   <  1:978678/251‑1 (MQ=255)
                           atatTACAAATCTGGCTAACTAACTGGCTGGTGCGCCATATCTCCTGGTGATTAATGGCGGTGTGGACGTTGAGAATATCCTTACGAACT‑TTGATCGCC‑ATGATGACTTCTTCCA‑GGGAACAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCC‑AGTGAGTTGCCGACCGCCAGGCC‑TAAATCGTCGTGGGTATGTACGGAAATAATGGCttt                                                                                                                                                                                                                   <  1:5536/251‑1 (MQ=255)
                              ttACAAATCTGGCTAACTAACTGGCTGGTGCGACATATCTCCTGGTGATTAATGGCGGTGTGGACGTTGAGAAAATCCTTACGAAAT‑TTGATCGCC‑ATGATGACTTCTTCCA‑GGGGACAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTTCCGCCGCC‑CGTGTGTTACCGCCCGCCAGGCC‑CAAATCGTCCTGGGTATGTACGGGAATAATGGCTTTGTc                                                                                                                                                                                                                >  1:822092/1‑251 (MQ=255)
                                 aaaaTCTGGCTAACTAACGGGCTGGTGCGCCATATCTCCTGGTGATTAATGGCGGTGTGGACGTTGAGAATATCCTTACGAACT‑TTGATCGCC‑ATGATGACTTCTTCCA‑GGGAACAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCC‑AGTGAGTTGCCGACCGCCAGGCC‑TAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGAt                                                                                                                                                                                                             <  1:368988/250‑1 (MQ=255)
                                     cctgtctaacaaaCTGGCTGGTGCGCCATATCTCCGGGTGATTAATGGCTGTGTGGACGGTGAGAATACCCTTACCAACT‑TTTAGCGCC‑ACGATGACTTCTTCCA‑GGGAACAGTTTCCGGCACCCTCGCCGACCCCGTTCATTGCGCCTTCCACCTGGCGTGCTCCGACATGTACCGCCGCC‑AGTGAGTGGCCGACCGCCAGGCC‑TAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTa                                                                                                                                                                                                         <  2:509456/246‑1 (MQ=255)
                                        ggCTAACTAACTGGCTGGTGCGCCATATCTCCTGGTGATTAATGGCGGTGTGGACGTTGAGAATATCCTTACGAACT‑TTGATCGCC‑ATGATGACTTCTTCCA‑GGGAACAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCC‑AGTGAGTTGCCGACCGCCAGGCC‑TAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAgg                                                                                                                                                                                                       <  1:620533/250‑1 (MQ=255)
                                                aactGGCTGGTGCGCCATATCTCCTGGTGATTAATGGCGGTGTGGGCGTTGAGAATATCCTTACGAACT‑TTTATCGCC‑ATGATGACTTCTTCCA‑GGGAACAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCC‑AAGGAGGTGCCGACCGCCAGGCCTCAAA‑CGTCGGGGGTATGGAAGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTc                                                                                                                                                                                              >  1:389803/1‑251 (MQ=255)
                                                aacgggctggTGCGCCATATCTCCTGGGGATTAATGGCGGTGTGGACGTTGAGAATATCCTTACGAACT‑TTGATCGCC‑ATGATGACTTCTTCCA‑GGGAACAGTTTCCGGCACGCTCGCCGATCCCGTGCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCC‑AGTGAGTTGCCGACCGCCAGGCC‑TAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGt                                                                                                                                                                                                <  1:651285/245‑1 (MQ=255)
                                                 actGGCTGGTGCGCCATATCTCCTGGTGATTAATGGCGGTGTGGACGTTGAGAATATCCTTACGAACT‑TTGATCGCC‑ATGATGACTTCTTCCA‑GGGAACAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCC‑AGTGAGTTGCCGACCGCCAGGCC‑TAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCa                                                                                                                                                                                             <  1:241646/251‑1 (MQ=255)
                                                    ggctggTGCGCCATATCTCCTGGTGATTAATGGCGGTGTGGACGTTGAGAATATCCTTACGAACT‑TTGATCGCC‑ATGATGACTTCTTCCA‑GGGAACAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCC‑AGTGAGTTGCCGACCGCCAGGCC‑TAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGttaat                                                                                                                                                                                            <  1:610529/249‑4 (MQ=255)
                                                        ggTGCGCCATATCTCCTGGTGATTAATGGCGGTGTGGGAGTTGAGAAAATCCTTACGAACT‑TTTATCGCC‑ATGATGACTTCTTCCA‑GGGAACAATTTTCGGCACGCTCGCCGATCCCCTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCC‑CGAGGGGTGCCGACCGCCAAGCC‑TCAATCGTCGTGGGTATGGACGGGAATAATGGCTTTGTCGATGTTTGGGACGCGTTCCTAccggg                                                                                                                                                                                      >  1:220219/1‑247 (MQ=255)
                                                        ggTGCGCCATATCTCCTGGTGATTAATGGCGGTGTGGACGTTGAGAATATCCTTACGAACT‑TTGATCGCC‑ATGATGACTTCTTCCA‑GGGAACAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCC‑AGTGAGTTGCCGACCGCCAGGCC‑TAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAgg                                                                                                                                                                                       <  1:101211/250‑1 (MQ=255)
                                                          tGCGCCATATCTCCTGGTGATTAATGGCGGTGTGGACGTTGAGAATATCCTTACGAACT‑TTGATCGCC‑ATGATGACTTCTTCCA‑GGGAACAGTTTCCGGCCCGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCC‑AGTGAGTTGCCGACCGCCAGGCC‑TAAATCGTCGTGGGGATGTACGGGAATAATGGCTTTGTCGATGTTAGGGACGCGTTCATACAAGCCg                                                                                                                                                                                    >  1:818736/1‑251 (MQ=255)
                                                            cgcCATATCTCCTGGTGATTAATGGCGGTGTGGACGTTGAGAATATCCTTACGAACT‑TTGATCGCC‑ATGATGCCTTCTTCCA‑GGGAACAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCC‑AGTGAGTTGCCGACCGCCAGGCC‑TAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCt                                                                                                                                                                                  <  1:342100/251‑1 (MQ=255)
                                                                atatCTCCTGGTGATTAATGGCGGTGTGGACGTTGAGAATATCCTTACGAACT‑TTGATCGCC‑CTGATGACTTCTTCCA‑GGGAACAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCC‑CGTGAGTTGCCGACCCCCAGGCC‑TAAATCGTCGTGGGGATTTACGGGAATAATGGCTTTGTCGATGTTAGGGACGGGTTCATACAGGCCGCtggtg                                                                                                                                                                              >  1:874006/1‑248 (MQ=255)
                                                                atatCTCCTGGTGATTAATGGCGGTGTGGACGGTGAGAATATCCTTACGAACT‑TTGATCGCG‑ATGTTGACTTCTTCCA‑GGGAACAGTGGCCGGCACGCTCGCCGATCCCGTTCATTGCGCTTTCTACCGGGCGTGCACCGGCAGGTACCGCCGCC‑AGTGAGTTGCGGACCGCCAGGCC‑TAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCtgatg                                                                                                                                                                              <  1:129762/251‑1 (MQ=255)
                                                                     tcCTGGTGATTAATGGCGGTGTGGACGTTGAGAATATCCTTACGAACC‑TTGATCGAC‑ATGTTGACCTCTTCCA‑GGGAACAGTTTCCGGCACGCCCGCCGATCACGTTTATTGCGCCTTCCCCCTGGAGTGCACCGGCCGGTACCGCCGCC‑AGTGAGTTGACGACCGCCAGGCC‑TAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTc                                                                                                                                                                          <  1:546862/250‑1 (MQ=255)
                                                                                   ggCGGTGTGGACGTTGAGAATATCCTTACGAACT‑TTGATCGCC‑ATGATGACTTCTTCCA‑GGGAACAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCC‑AGTGAGTTGCCGTCCGCCAGGCC‑TAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACT‑CAAAc                                                                                                                                                           <  1:580280/250‑1 (MQ=255)
                                                                                    gCGGTGTGGACGTTGAGAATATCCTTACGAACT‑TTGATCGCC‑ATGATGACTTCTTCCA‑GGGAACAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCCCCGGCATGTACCGCCGCC‑AGTGAGTTGCCGACCGCCAGGCC‑TAAAGCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACT‑CAAACgg                                                                                                                                                         <  1:165691/251‑1 (MQ=255)
                                                                                    gCGGTGTGGACGTTGAGAATATCCTTACGAACT‑TTGATCGCC‑ATGATGACTTCTTCCA‑GGGAACAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCC‑AGTGAGTTGCCGACCGCCAGGCC‑TAAATCGTCGTGGGGATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATTAAGGCCGCTGATGATTCCGGCGAACT‑CAAACgg                                                                                                                                                         >  2:207263/1‑251 (MQ=255)
                                                                                       gtgtgtACGTTGAGAATATCCTTACGAACT‑TTGATCGCC‑ATGATGACTTCTTCCA‑GGGAACAGTTTCCGGCCCGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCC‑AGTGAGTTGCCGACCGCCAGGAC‑ACACTCGTCGTGGGTATGTAAGGAAATAATGGCTTTGACGTTGTTAGGGACGCGTTCATACGTGCCGCTTATGGTTACGGCGGACTACAAACgcc                                                                                                                                                        >  2:638138/1‑248 (MQ=255)
                                                                                          tgGACGTTGAGAATATCCTTACGAACT‑TTGATCGCC‑ATGATGACTTCTTCCA‑GGGAACAGTTTCCGGCACGCTCGCCGATCCCGTTCCTTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCC‑AGTGAGTTGCCGACCGCCAGGCC‑TAAATCGGCGTGGGTATGTACGGAAATCATGGCTTTGTCGATGTTAAGGACGCGTTCATACAGGCCGCTGCAGATGCCGGCGAACT‑CAAACGGCCTGgt                                                                                                                                                   >  2:382669/1‑251 (MQ=255)
                                                                                              cGTTAAGAATATCCTTACGACCC‑TTGATCGCC‑ATGATGACTTCTTCCA‑GGGAACAGTTTCCGGACCGCTCGCCGATCCCGTACATGGCGCCTGCCACCTGGCGTGCCCCGGCATGTCCCGCCGCC‑AGTGAGTTGCCGACCGCCAGGCC‑TAAAACGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTGCGCGTTCATACAGGCCGCTGATGATTCCGGCGAACT‑CAAACGGCATGGTGTAg                                                                                                                                               <  1:581368/251‑1 (MQ=255)
                                                                                                 gtaaaaTATCCTGACGA‑CTGTTGATCGCCTAT‑ATGCCTTCTTCCG‑GGTAACAGTTTCCGGCCCGCCCTCCGAGCCAGTCCATGTCGCCTTCCCCCTGGTGTGCCCCGTCATGTACCGCCGCC‑AGTGAGTTGCCGACCGCCAGGCC‑TAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACT‑CAAACGGCATGGTGTAGccc                                                                                                                                            <  2:505209/247‑1 (MQ=255)
                                                                                                  gagaATATCCTTACGAACT‑TTGATCGCC‑ATGATGACTTCTTCCA‑GGGAACAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCC‑AGTGAGTTGCCGACCGCCAGGCC‑TAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACT‑CAAACGGCATGGTGTAGccc                                                                                                                                            <  1:567961/250‑1 (MQ=255)
                                                                                                   agaATATCCTTACGAACT‑TTGATCGCC‑ATGATGACTTCTTCCA‑GGGAACAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCC‑AGTGAGTTGCCGACCGCCAGGCC‑TAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACT‑CAAACGGCATGGTGTAGCCCAc                                                                                                                                          <  1:129838/251‑1 (MQ=255)
                                                                                                   agaATATCCTTACGAACT‑TTGATCGCC‑ATGATGACTTCTTCCA‑GGGAACAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCC‑AGTGAGTTGCCGACCGCCAGGCC‑TAAATCGTCGTGGGTATGTAAGGAAATAATGGCTTTGTCGCTGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACG‑CAACCGGCCTGgtgtgaccccc                                                                                                                                          >  2:695316/1‑244 (MQ=255)
                                                                                                        atCCTTACGAACT‑TTGATCGCC‑ATGATGACTTCTTCCA‑GGGAACAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCC‑AGTGAGTTGCCGACCGCCAGGCC‑TAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACT‑CAAACGGCATGGTGTAGCCCACGgtgt                                                                                                                                     >  1:584551/1‑251 (MQ=255)
                                                                                                          ccTTACGAACT‑TTGATCGCC‑ATGATGACTTCTTCCA‑GGGAACAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCC‑AGTGAGTTGCCGACCGCCAGGCC‑TAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACT‑CAAACGGCATGGTGTAGCCCACGGTGTc                                                                                                                                    <  1:888318/250‑1 (MQ=255)
                                                                                                           cTTACGAACT‑TTGATCGCC‑ATGATGACTTCTTCCA‑GGGAACAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCC‑AGTGAGTTGCCGACCGCCAGGCC‑TAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGATCATACAGGCCGCTGATGATTCCGGCGAACT‑CAAACGGCATGGTGTAGCCCACGGGGTCCg                                                                                                                                  >  1:109664/1‑251 (MQ=255)
                                                                                                                aaaCT‑TTGACCGCC‑ATGATTACTTCTTCCA‑GTGAACATTTTCCGGCACGCCCCCCGATCCCGTTCATTGCGCCGTCCACCTGGCGTGCACCGGCATGTACCGCCGCC‑AGTGAGTTGCCGACCGCCAGGCC‑TAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACT‑CAAACGGCATGGTGTAGCCCACGGTGTCCGGAATg                                                                                                                             <  1:65622/250‑1 (MQ=255)
                                                                                                                    t‑ttGATCGCC‑ATGATGACTTCTTCCA‑GGGAACAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCC‑AGTGAGTTGCCGACCGCCAGGCC‑TAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACT‑CAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTg                                                                                                                          >  1:113232/1‑250 (MQ=255)
                                                                                                                       tttgcccccagggtgtcttcttCCA‑GGGACCATTTTCCGGCACGCTCGCCGACCCCGTTCATTGCGCCTTCCACCTGGCGGGCACCGGCATGTACCGCCGCC‑AGTGAGTTGCCGACCGCCAGGCC‑TAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACT‑CAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGa                                                                                                                         <  2:247489/234‑1 (MQ=255)
                                                                                                                       tGATCGCC‑ATGATGACTTCTTCCA‑GGGAACAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCC‑AGTGAGTTGCCGACCGCCAGGCC‑TAAATCGTCGTGGGTATGCACGGAAATAAAGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGACCT‑CAAACGGCATGGTATAGCCCAAGGCGTCCGGAATGTTgttg                                                                                                                       >  2:424175/1‑248 (MQ=255)
                                                                                                                        gaccGCC‑ATGATGCCTTCTTCCA‑TGGAACAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCGCCTTGCTTGCACCGGCATGTACCGCCGCC‑AGTGAGTTGCCGACCGCCAGGCC‑TAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGTCCGCTGATGATTCCGGCGAACT‑CAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGAtg                                                                                                                       <  1:965507/247‑1 (MQ=255)
                                                                                                                         aTCGCC‑ATGATGACTTCTTCCA‑GGGAACAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCC‑AGTGAGTTGCCGACCGCCAGGCC‑TAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGGTGGTAGGTACGCGGTCATACAGGCCGCTGATGATTACGGCGAACT‑CAAACGGCATGGTGTAGACCACGGTGGCCGGGATGTTGAtggg                                                                                                                     >  2:22213/1‑250 (MQ=255)
                                                                                                                            gCC‑ATGATGACTTCTTCCA‑GGGAACAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCC‑AGTGAGTTGCCGACCGCCAGGCC‑TAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACT‑CAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGAtggtggg                                                                                                                  >  1:134568/1‑250 (MQ=255)
                                                                                                                              ccatgtgtgcttctcCCA‑GGGAACAGTTTGCGGCACGCGCGCCGACCCCGTTCATTTCGCCTTCCACCGGGCTAGCATCGGCATGTACCGCCGCC‑AGTGAGTTGCCGACCGCCAGGCC‑TAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACT‑CAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGAtggtggtg                                                                                                                 <  2:112423/242‑1 (MQ=255)
                                                                                                                              c‑ATGATGACTTCTTCCA‑GGGAACAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCC‑AGTGAGTTGCCGACCGCCAGGCC‑TAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACT‑CAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGAtggtggtgg                                                                                                                <  1:470834/251‑1 (MQ=255)
                                                                                                                                   atgaCTTCTTCCA‑GGGAAAAGTTTCCGGCACGCTCGCCGCTCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCC‑AGTGAGTTGCCGCCCGCCAGGCC‑GAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGGTCATACAGGCCGCTGATGATTCCGGCGAACT‑CAACCAGCATGGTGTAGACCAAGGTGTCCGGAATGTTGATGGGGGTGGCAcg                                                                                                            >  2:782570/1‑250 (MQ=255)
                                                                                                                                      aCTTCTTCCA‑GGGAACAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCAGCC‑CGTGAGTTGCCGACCGCCAGGCC‑TAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTATCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACT‑AAAACGGAATGGTGTAGCCCACGGAGTCCGGAATGTTGCTGGTGGTTGCACCGGc                                                                                                         >  1:900292/1‑251 (MQ=255)
                                                                                                                                         tcttcCA‑GGGAACAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCC‑AGTGAGTTGCCGACCGCCAGGCC‑TAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACT‑CAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCAtt                                                                                                      >  1:10766/1‑251 (MQ=255)
                                                                                                                                          cttcCA‑GGGAACAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCC‑AGTGAGTTGCCGACCGCCAGGCC‑TAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACT‑CAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCAtt                                                                                                      >  1:36874/1‑250 (MQ=255)
                                                                                                                                                              ggCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCC‑AGTGAGTTGCCGACCGCCAGGCC‑TAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACT‑CAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGGGGTGGCACCGGCATTAATCGCCGCTTCGACCACTc                                                                                  >  1:767566/1‑251 (MQ=255)
                                                                                                                                                               gCACGCTCGCCGATCCCGATCATTTCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCC‑AGTGAGTTGCCGACCGCCAGGCC‑TAAATCGTAGTGGGTATGTACGGTAATAATGGCTTTGTCGCTGTTAGGTACGCGTTCATAAAGGCCGCTGATGATTCCGGCGAACT‑AAAAAGGCATGGGGTAGCACACGGTGTCCGGCATGTTTGTGGTGGTGGCACCGGCATTAATCCTCGCTTCGAACACTcc                                                                                 >  2:799084/1‑250 (MQ=255)
                                                                                                                                                                cACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCC‑AGTGAGTTGCCGACCGCCAGGCC‑TAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACT‑CAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTcgc                                                                                >  1:304294/1‑251 (MQ=255)
                                                                                                                                                                 aCGCTCGCCGACCCCGTTCATTGCTCCTCCCACCTGGCGGGCTCCGGCATGTACCGCCTCC‑AGTGAGTTGCCGACCGCCAGGCC‑TAAAACGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACT‑CAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTcgcg                                                                               <  1:565446/251‑1 (MQ=255)
                                                                                                                                                                      cGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCC‑AGTGAGTTGCCGACCGCCAGGCC‑TAAATCGTCGTGGGAATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACT‑CAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCgcgca                                                                             >  1:69832/1‑247 (MQ=255)
                                                                                                                                                                       tccGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCC‑ATTGAGTTGCCGACCGCCAGTCC‑TCAATACTCGTGGGTATGTACGGCAATAATGGCTTTGACGATGTTATGTACGCGTTCATACAGGCCGCTTATGATACCGGCGAACT‑CAAACGTCAGGGTGTAGCCACCGGTGTCAGGATTTTTAGTGGTGGAGGCACCGGCATTACTCGCATCTTCGACCCCTcgcgac                                                                             >  1:654259/2‑245 (MQ=255)
                                                                                                                                                                       gCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCC‑AGTGAGTTGCCGACCGCCCGGCC‑TAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGTTGTTAGGTACGCGTTCCTACAGGCCGCTGATGATTCCGGCGAACT‑CAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATAGCCGCTTCGCCCACTcgcgaccgct                                                                         >  1:654250/1‑245 (MQ=255)
                                                                                                                                                                        ccgttCCCGTTCATTGCGCCGTCCACCTGGCGGGCACCGGCATGTCCAGCCGCT‑AGTGAGTTGCCGACCGCCAGGCC‑TAAAGCGTCGTGGGTATGTACGGAAAGAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGTCGCTGATGATTCCGGCGAACT‑CAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGTCCACTCGCGACAGATc                                                                        <  1:547671/247‑1 (MQ=255)
                                                                                                                                                                          gacccgtttctgtGCGCCTTCCACCTGGCGTGCCCGGGGGTGTACCGCCGCC‑AGTGAGTTGCCGACCGCCAGTCC‑TAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACT‑CAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATctg                                                                      <  2:509709/239‑3 (MQ=255)
                                                                                                                                                                          gATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCC‑AGTGAGTTGCCGACCGCCAGGCC‑TAAATCGTCGTGGGGATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACT‑CAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGCGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGcc                                                                             >  2:165511/1‑244 (MQ=255)
                                                                                                                                                                              ccGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCC‑AGTGAGTTGCCGACCGCCAGGCC‑TAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACT‑CAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATCGGCAAt                                                                  <  1:202810/251‑1 (MQ=255)
                                                                                                                                                                                gatCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCC‑AGTGCGTTGCCGACCGCCAGGCC‑TAAATCGTCGTGGGCATGTACGGAAATAATGACTTTGTCGCTGATAGGCACGCGTTCATACGGGCCGCTGCTGATACCGGCGAACT‑CAAACGAAATGGTGAAGCCCACGGTGTCCGGAATGTTGACGGTGGTGGCAACGGCATTACTCGCCGCTTCGACACCTCgcgcacgataggcaaagg                                                                >  1:897730/3‑237 (MQ=255)
                                                                                                                                                                                  tCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCC‑AGTGAGTTGCCGACCGCCAGGCC‑TAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACT‑CAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACCCGCGCCAGATCGGCAATgggg                                                              >  2:504106/1‑250 (MQ=255)
                                                                                                                                                                                   cATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCC‑AGTGAGTTGCCGACCGCCAGGCC‑TAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACT‑CAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGGGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATCGGCAATGGgtg                                                             >  1:135678/1‑251 (MQ=255)
                                                                                                                                                                                          ccTTCCACCTGGCGTGCACCGGCATGTACCGCCGCC‑AGTGAGTTGCCGACCGCCAGGCC‑TAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACT‑CAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATCGGCAATGGGTGTACGccc                                                      <  1:700776/251‑1 (MQ=255)
                                                                                                                                                                                          ccTTCCACCTGGCGTGCACCGGCATGTACCGCCGCC‑AGTGAGTTGCCGACCGCCAGGCC‑TAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACT‑CAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATCGGCAATGGGTGTACGccc                                                      <  1:778961/251‑1 (MQ=255)
                                                                                                                                                                                            cgccccctggcttcccccGGAATGTACCGCCGCC‑AAGGAGTTGCCGACCGCCAGGCC‑TAAACCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGGTCATACAGGCCGCTGATGATTCCGGCGAACT‑CAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATCGGCAATGGGTGTACGCCCg                                                     <  2:295637/234‑1 (MQ=255)
                                                                                                                                                                                             tCCACCTGGCGTGCACCGGCATGTACCGCCGCC‑AGTGAGTTGCCGACCGCCAGGCC‑TAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACT‑CAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATCGGCAATGGGTGTACGCCCg                                                     <  1:299467/249‑1 (MQ=255)
                                                                                                                                                                                              ccgccgggCGTGCACCGGCATGTACCGCCGCC‑AGTGAGTTGCCGACCGCCAGGCC‑TAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACT‑CAAACGGCATGGTGTAGCCCGCGGTGTCCGGAATGTTGATGGTGGTGGCACCTGCATTAATCGCCGCTTCGACCACGCGCGCCAGATCGGCAATGGGTGTACGCCCGGCa                                                  <  1:565160/245‑1 (MQ=255)
                                                                                                                                                                                               cACCTGGCGTGCACCGGCATGTACCGCCGCC‑AGTGAGTTGCCGACCGCCAGGCC‑TAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACT‑CAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATCGGCAATGGGTGTACGCCCGGCAt                                                 >  1:365108/1‑251 (MQ=255)
                                                                                                                                                                                                 cagggCGTGAACCGTGATGTACCGTCGCC‑AGTGAGTTGCCGACCTCCAGGAC‑TAAATCGGCGTGGGTATGTACGGAAATAAGGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACT‑CAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATCGGCAATGGGTGTACGCCCGGCATCt                                               <  2:870563/248‑1 (MQ=255)
                                                                                                                                                                                                  cgggCGTGCACCGGCATGTACCGCCGCC‑AGTGAGTTGCCGACCGCCAGGCC‑TAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACT‑CAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATCGGCAATGGGTGTACGCCCGGCATCtt                                              <  1:382669/249‑1 (MQ=255)
                                                                                                                                                                                                   gggCGTGCACCGTCTGGTACCGCCGCC‑AGTGGGTTGCCGACCGCCAGGCC‑TAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACT‑CAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATCGGCAATGGGTGTACGCCCGGCATCTTc                                             <  2:602309/250‑1 (MQ=255)
                                                                                                                                                                                                   cggCGTGCACCGGCATGTACCGCCGCC‑AGTGAGTTGCCGACCGCCAGGCC‑TAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACT‑CAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATCGGCAATGGGTGTACGCCCGGCATCTTc                                             <  2:233728/250‑1 (MQ=255)
                                                                                                                                                                                                         gcatcgccATGTACCGCCGCC‑AGTGAGTGGCCGACCGCCAGGCC‑TAAATCGTCGTTGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACT‑CAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCTCTTCGACCACTCGCGCCAGATCGGCAATGGGTGTACGCCCGGCATCTTCGCAAg                                        <  2:789897/243‑1 (MQ=255)
                                                                                                                                                                                                         gCACCGGCATGTACCGCCGCC‑AGTGAGTTGCCGACCGCCAGGCC‑TAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACT‑CAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGCCCACTCGCGCCAGTTCGGCAATGGGTGTACGCCCGGCATCTTCGCa                                          >  1:29740/1‑248 (MQ=255)
                                                                                                                                                                                                           aCCGGCATGTACCGCCGCC‑AGTGAGTTGCCGACCGCCAGGCC‑TACATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACT‑CAAACGGCATGGTGTCGCCCACGGTGTCCGGGATGTTGATGGTGGGGGCACCGGCATTAATCGCCGCTTCGACCACCCGCGCCAGAACGGCAATGGGGGTACGCCCGGCATCCTCGCAAGaa                                      >  2:977034/1‑250 (MQ=255)
                                                                                                                                                                                                           aCCGGCATGTACCGCCGCC‑AGTGAGATTCCGACCGACAGGCC‑TAAAACGTCGAGGGTATTTACGGAAATAATGGCTTTGTCGATGTTAGGGACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACA‑CAAACGGCATGGTGTAGCCCACGGTGACCGGAATGTTGATGGGGGTGGCACCGGCACTCATCGACGCTTCGACCACTCGCGCCAGATCCGCCATGGGTGAACACCCGGccacttcggaagga                                      >  2:137760/1‑237 (MQ=255)
                                                                                                                                                                                                            ccGGCATGTACCGCCGCC‑AGTGAGTTGCCGACCGCCAGGCC‑TAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACT‑CAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATCGGCAATGGGTGTACGCCCGGCATCTTCGCAAGaaa                                     >  1:773805/1‑250 (MQ=255)
                                                                                                                                                                                                               gcaggTACCGCCGCC‑AGTGAGTTGCCGACCGCCAGGCC‑TAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACT‑CAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATCGGCAATGGGTGTACGCCCGGCATCTTCGCAAGAAAATTc                                 <  2:874006/247‑1 (MQ=255)
                                                                                                                                                                                                                cATGTACCGCCGCC‑AGTGAGTTGCCGACCGCCAGGCC‑TAAATCGTCGTGGGTATGTAAGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACT‑CAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCCCTCGCGCCAGATCGGCAATGGGTGTACGCCCGGCATCTTCGAAAGACAATTc                                 >  2:150686/1‑250 (MQ=255)
                                                                                                                                                                                                                    tACCGCCGCC‑AGTGGGTTGCCGACCGCCGGGCC‑TGAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACT‑CAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATCGGCAATGGGTGTACGCCCGGCATCTTCGCAAGAAAATTCAAcat                            >  1:223188/1‑251 (MQ=255)
                                                                                                                                                                                                                        cccgcc‑AGTGAGTTGCCGACCGCCAGGCC‑TAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACT‑CAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATCGGCAATGGGTGTACGCCCGGCATCTTCGCAAGAAAATTCAACatcatc                        <  2:226592/250‑1 (MQ=255)
                                                                                                                                                                                                                          cgcc‑AGTGAGTTGCCGACCGCCAGGCC‑TAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACT‑CCAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGGGGCCTCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATCGGCAATGGGTGTACGCCCGGCATCTTc                                             >  2:274495/1‑228 (MQ=255)
                                                                                                                                                                                                                          cgcc‑AGTGAGTTGCCGACCGCCAGGCC‑TAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACT‑CAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATCGGCAATGGGTGTACGCCCGGCATCTTc                                             <  1:274495/228‑1 (MQ=255)
                                                                                                                                                                                                                            cc‑AGTGAGTTGCCGACCGCCAGGCC‑TAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACT‑CAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATCGGCAATGGGTGTACGCCCGGCATCTTCGCAAGAAAATTCAACATCATCGgtg                    <  1:439255/251‑1 (MQ=255)
                                                                                                                                                                                                                            cc‑AGTGAGTTGCCGACCGCCAGGCC‑TAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACT‑CAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATCGGCAATGGGGGTACGCCCGGCCTCTTCGCAAGAAAATTCAACTTCCTCGgtg                    >  2:883312/1‑251 (MQ=255)
                                                                                                                                                                                                                               aGTGAGTTGCCGACCGCCAGGCC‑TAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACT‑CAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATCGGCAATGGGTGTACGCCCGGCATCTTCGCAAGAAAATTCAACATCATCGGTGTa                  <  1:674821/251‑1 (MQ=255)
                                                                                                                                                                                                                                   aGTTGCCGACCGCCAGGCC‑TAAATCGTCGTGGGTATATACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACT‑CAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATCGGCAATGGGTGTACGCCCGGCATCTTCGCAAGAAAATTCAACATCATCGGTGTAATTa              <  1:936111/251‑1 (MQ=255)
                                                                                                                                                                                                                                      tgccGACCGCCAGGCC‑TAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACT‑CAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATCGGCAATGGGTGTACGCCCGGCATCTTCGCAAGAAAATTCAACATCATCGGTGTAATTACg            <  1:720139/248‑1 (MQ=255)
                                                                                                                                                                                                                                       gccGACCGCCAGGCC‑TAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACT‑CAAACGGCATGGTGTAGCCCACGGTGTCCGGCATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATCGGCACTGGGTGTACGCCCGGCATCTTCGCAAGAAAATTCAACATCATCGGTGTAATTACggg          >  1:567853/2‑251 (MQ=255)
                                                                                                                                                                                                                                       gccGACCGCCAGGCC‑TAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACT‑CAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATCGGCAATGGGTGTACGCCCGGCATCTTCGCAAGAAAATTCAACATCATCGGTGTAATTACgg           <  1:646896/249‑1 (MQ=255)
                                                                                                                                                                                                                                        ccGACCGCCAGGCC‑TAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACT‑CAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATCGGCAATGGGTGTACGCCCGGCATCTTCGCAAGAAAATTCAACATCATCGGTGTAATTACGGgc         <  1:415575/251‑1 (MQ=255)
                                                                                                                                                                                                                                        ccGACCGCCAGGCC‑TAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACT‑CAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATCGGCAATGGGTGTACGCCCGGCATCTTCGCAAGAAAATTCAACATCATCGGTGTAATTACGGgc         <  1:972162/251‑1 (MQ=255)
                                                                                                                                                                                                                                          gACCGCCAGGCC‑TAAATCGTCGTGGGTATGTAAGGAAATAATGGCTTTGTCGATGTTAGGTACGAGTTCATACAGGCCGCTGATGATTCCGGCGAAAT‑CAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGATTCGACCACTCGCGCCAGATCGGCAATGGGCGACCGCCCGGCATCTTCGCAAGAACATTCAACATCATCGGTGTAATTACGGgcgg       >  2:275775/1‑250 (MQ=255)
                                                                                                                                                                                                                                              gCCAGGCC‑TAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACT‑CAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATCGGCAATGGGTGTACGCCCGGCATCTTCGCAAGAAAATTCAACATCATCGGTGTAATTACGGGCGCGttt   >  1:242063/1‑251 (MQ=255)
                                                                                                                                                                                                                                                cAGGCC‑TAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGGTGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACT‑CAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGGCCACTCGCGCCAGATCGGCAATGGGTGTACGCCCGGCATCTTCGCAAGAAAATTCAACATCATCGGTGTAATTACGGGCGCGTTTc  >  1:394801/1‑250 (MQ=255)
                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                    
ACAATGGCTTTGTTTGCCGGGATCGGCATATTACAAATCTGGCTAACTAACTGGCTGGTGCGCCATATCTCCTGGTGATTAATGGCGGTGTGGACGTTGAGAATATCCTTACGAACT‑TTGATCGCC‑ATGATGACTTCTTCCA‑GGGAACAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCC‑AGTGAGTTTCCGACCGCCAGGCC‑CAAATCGTCGTGGGTATGTACGGAGATAATGGCTTTGTCGATGTTAGGCACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACT‑CAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATCGGCAATGGGTGTACGCCCGGCATCTTCGCAAGAAAATTCAACATCATCGGTGTAATTACGGGCGCGTTTC  >  NC_000913/82667‑83152

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑