Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 82,894 T→G G212G (GGA→GGC)  leuA ← 2‑isopropylmalate synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091382,8940TG98.1% 306.2 / NA 104G212G (GGA→GGC) leuA2‑isopropylmalate synthase
Reads supporting (aligned to +/- strand):  ref base T (2/0);  new base G (42/59);  total (45/59)
Fisher's exact test for biased strand distribution p-value = 1.80e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.76e-01

Only 100 of 104 total aligned reads displayed.

GAATGCGCCGCTGCCAACAATGGCTTTGTTTGCCGGGATCGGCATATTACAAATCTGGCTAACTAACTGGCTGGT‑GCGCCATATCTCCTGGTGATTAATGGCGGTGTGG‑ACGTTGAGAATATCCTTACGAACT‑TTGATCGCC‑ATGATGACTTCTTCCA‑GGGAACAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCC‑AGTGAGTTTCCGACCGCCAGGCC‑CAAATCGTCGTGGGTATGTACGGAGATAATGGCTTTGTCGATGTTAGGCACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACT‑CAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATCGGCAATGGGTGTACGCCCGGCATCTTCGCAAGAAAATTCAACATCATCGGTGTAATTA  >  NC_000913/82651‑83140
                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                        
gAATGCGCCGCTGCCAACAATGGCTTTGTTTGCCGGGATCGGCATATTACAAATCTGGCTAACTAACTGGCTGGT‑GCGCCATATCTCCTGGTGATTAATGGCGGTGGGG‑ACGTTGAGAATAACCTTATGGACT‑TTGGTCGCC‑ATGATGGCTTCTTTCA‑GGGAACAGTTTCCGGCGCGCTCGCCGCTCCCGCTCATTGCGCCTTCCCCCCGGCGTGCACCGGCGTGTACCGCCCCC‑CGCGAGTTGCCGAccc                                                                                                                                                                                                                                                   >  1:137876/1‑250 (MQ=255)
   ggcgcTGCTGCCAACAATGGGTTTGTTTCCCGGTATCGGCATATTTAAAATCTGGCTACCTA‑CTTGGTGGTTGCCCCATACCTCCGGGTGATTAAGGGCG‑TTTGGTACGTTGAGAATATCCTTACGAACT‑TTGATCCCC‑ATGATGACTTCTCCCA‑GTGATCATTTTCCGGCTCCCTCGCGGTTCCTGTTCATTGCGCCTTCCTCCTTGCTTTCACCGGCATGTACCGCCTCC‑ATTTAGTTGCCGACCGCCa                                                                                                                                                                                                                                                <  2:563678/250‑1 (MQ=255)
        cgcTGCCAACAAGGGTTTTGTTTGCCGGGATCGTCATATTACAAATCTTGCTAACTAACTGGCTGGT‑GCGCCATATCTCCTGGTGATTAATGGCGGTGTGG‑ACGTTGAGAATATCCTTACGAACT‑TTGATCGCC‑ATGATGACTTCTTCCA‑GGGAACAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCC‑AGTGAGTTGCCGACCGCCAGGcct                                                                                                                                                                                                                                           <  2:746497/251‑2 (MQ=255)
            gcaaaaaaTGGCTTGGTTTGCCGGGAACGGCATATGTCAAATGTGTCTAACTAACGGGCTGTT‑GCGCCATATCTCCTGGTGATTAATGGGGGTTTGG‑ACGTTGAGAATATCCTTACGAACT‑TGGATCGCC‑ATGATGACTTCTTCCA‑GGGAACAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCAACGGCATGTACCGCCGCC‑AGTGAGTTGCCGACCGCCAGGcctaa                                                                                                                                                                                                                                         <  2:144644/243‑4 (MQ=255)
              caacaaTGGCTTTGTTTGCCGGGATCGGCATATTACAAATCTGGCTAACTAACTGGCTGGT‑GCGCCATATCTCCTGGTGATTAATGGCGGTGTGG‑ACGTTGAGAATATCCTTACGAACT‑TTGATCGCC‑ATGATGACTTCTTCCA‑GGGAACAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCCTGTAACGCCGCC‑CGTGAGTTGCCGACCGCCAGGcctcaatcg                                                                                                                                                                                                                                     >  1:317109/1‑244 (MQ=255)
                  aaTGGCTTTGTTTGCCGGGATCGGCATATTACAAATCTGGCTAACTAACTGGCTGGT‑GCGCCATATCTCCTGGTGATTAATGGCGGTGTGG‑ACGTTGGGAATATCCTTTCGAACT‑TTTATCGCC‑CTGATGACTTCTTTCC‑GGGAACAGTTTTCGGCCAGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCCTGTACCCCCGCCCAG‑GAGTTGCCGACCGCCAGGCC‑CAAAtactcgg                                                                                                                                                                                                                                >  1:779105/1‑245 (MQ=255)
                    tGGCTTTGTTTGCCGGGATCGGCATATTACAAATCTGGCTAACTAACTGGCTGGT‑GCGCCATATCTCCTGGTGATTAATGGCGGTGTGG‑GAGTTGAGAATATACTTACAAACT‑TTTATAGCC‑CTGATGACTTTTTCCC‑GGGAAAAGTTTTCGGCACGCTCTCCCAGCCCGTTCATTGCGCCTTCCCCCTGGCGGGCACCGGCATGGTCCGCCGCC‑AGTGAGTTGGCGACCCCCAGGCC‑CAAAacgtggggg                                                                                                                                                                                                                              >  2:674821/1‑246 (MQ=255)
                          tgtttgCCGGGATCGGCATATTACAAATCTGGCTAACTAACTGGCTGGT‑GCGCCATATCTCCTGGTGATTAATGGCGGTGGGG‑ACGTTGAGAATATCCTTACGAACT‑TTGGTCGCC‑ATGATGACTTCTTCCAAGGGAA‑AGATTCCGGCACGCTCGCCGATACCGTTCATTGCGCCTTCCACCCGGCGTGCACCGGGATGTACCGCCGCC‑CGTGGGTTTCCGCCCGCCCAGAC‑CAAATCGACGTgggggtgt                                                                                                                                                                                                                        >  1:259284/1‑246 (MQ=255)
                              ggCCGGGATCGGCATATTACAAAGCTGGCTAACTAACTGGCTGGT‑GCGCCATATCTCCTGTTGATTAATGGCGGTGTGG‑ACGTTGAGAATATCCTTACGAACT‑GTGATCGCC‑ATGATGACTTCTTCCA‑GGGAACAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCC‑AGTGAGTTGCCGACCGCCAGGCC‑TAAATCGTCGTGGGTATGTACgg                                                                                                                                                                                                                    <  2:598816/250‑1 (MQ=255)
                                ccGGGATCGGCATATTACAAATCTGGCTAACTAACTGGCTGGT‑GCGCCATATCTCCTGGTGATTAATGGCGGTGTGG‑ACGTTGAGAATATCCTTACGAACT‑TTGATCGCC‑ATGATGACTTCTTCCA‑GGGAACAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCC‑AGTGAGTTGCCGACCGCCAGGCC‑TAAATCGTCGTGGGTATGTACGgaa                                                                                                                                                                                                                  <  1:545488/251‑2 (MQ=255)
                                  gggATCGGCTTATTACAAATCTGGCTAACTAACGGGCTGGG‑GCGCCATATTCCCTGGGGATTAAGGGCGGTGTGG‑ACGTTGAGAATATCCTGACGAACT‑TTGATCGCC‑ATGATTACTTCTTCCA‑GGGAACAGTTTCCGGCACGGTCGCCGATCCCGTTCATTGCGCCTTCCTCCTGTCGTGCACCGGCTTGTACCGCCGCC‑AGTGAGTTGCCGACCGCCAGGCC‑TAAATCGTCGTGGGTATGTACGgaaat                                                                                                                                                                                                                <  1:114215/251‑4 (MQ=255)
                                    gaccGGCATATTACAAATCTGGCTAACTAACTGGCTGGT‑GCGCCATATCTCCTGGTGATTAATGGCGGTGTGG‑ACGTTGAGAATATCCTTACGAACT‑TTGATCGCC‑ATGATGACTGCTGCCA‑GGGAACAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCC‑AGTGAGTTGCCGACCGCCAGGCC‑TAAATCGTCGTGGGTATGTACGGAAATaa                                                                                                                                                                                                              <  2:907294/248‑1 (MQ=255)
                                    gATCGTCATATTACAAATCTGGCTAACTAACTGGCTGGT‑GCGCCATATCTCCTGGTGATTAATGGCGGTGTGG‑ACGTTGAGAATATCCTTACGAACT‑TTGATCGCC‑ATGATGACTTCTTCCA‑GGGAACAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCC‑AGTGAGTTGCCGACCGCCAGGCC‑TAAATCGTCGTGGGTATGTACGGAAATaa                                                                                                                                                                                                              <  1:928958/251‑1 (MQ=255)
                                         gCATATTACAAATCTGGCTAACTAACTGGCTGGT‑GCGCCATAGCTCCTGGTGATTAATGGCGGTGTGG‑ACGTTGAGAATATCCTTACGAACT‑TTGATCGCC‑ATGATGACTTCTTCCA‑GGGAACAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCC‑AGTGAGTTGCCGACCGCCAGGCC‑TAAATCGTCGTGGGTATGTACGGAAATAATGGCt                                                                                                                                                                                                         <  2:295783/251‑1 (MQ=255)
                                          cATATTACAAATCTGGCTAACTAACTGTCTGGT‑GCGCCATATCTCCTGGGGATTAATGGCGGTGTGG‑ACGTTGAAAATATCCTTACGAACT‑TTGATCGCC‑ATGATGACTTCTTCCA‑GGGAACAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCCCCTGGCGTGCACCGGCATGTACCGCCGCC‑AGTGAGTTGCCGACCGCCAGGCC‑TAAATCGTCGTGGGTATGTACGGAAATAATGGCtt                                                                                                                                                                                                        <  2:961729/251‑1 (MQ=255)
                                           atatTACAAATCTGGCTAACTAACTGGCTGGT‑GCGCCATATCTCCTGGTGATTAATGGCGGTGTGG‑ACGTTGAGAATATCCTTACGAACT‑TTGATCGCC‑ATGATGACTTCTTCCA‑GGGAACAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCC‑AGTGAGTTGCCGACCGCCAGGCC‑TAAATCGTCGTGGGTATGTACGGAAATAATGGCttt                                                                                                                                                                                                       <  1:5536/251‑1 (MQ=255)
                                           atatTACAAATCTGGCTAACTAACTGGCTGGT‑GCGCCATATCTCCTGGTGATTAATGGCGGTGTGG‑ACGTTGAGAATATCCTTACGAACT‑TTGATCGCC‑ATGATGACTTCTTCCA‑GGGAACAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCC‑AGTGAGTTGCCGACCGCCAGGCC‑TAAATCGTCGTGGGTATGTACGGAAATAATGGCttt                                                                                                                                                                                                       <  1:978678/251‑1 (MQ=255)
                                              ttACAAATCTGGCTAACTAACTGGCTGGT‑GCGACATATCTCCTGGTGATTAATGGCGGTGTGG‑ACGTTGAGAAAATCCTTACGAAAT‑TTGATCGCC‑ATGATGACTTCTTCCA‑GGGGACAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTTCCGCCGCC‑CGTGTGTTACCGCCCGCCAGGCC‑CAAATCGTCCTGGGTATGTACGGGAATAATGGCTTTGTc                                                                                                                                                                                                    >  1:822092/1‑251 (MQ=255)
                                                     cctgtctaacaaaCTGGCTGGT‑GCGCCATATCTCCGGGTGATTAATGGCTGTGTGG‑ACGGTGAGAATACCCTTACCAACT‑TTTAGCGCC‑ACGATGACTTCTTCCA‑GGGAACAGTTTCCGGCACCCTCGCCGACCCCGTTCATTGCGCCTTCCACCTGGCGTGCTCCGACATGTACCGCCGCC‑AGTGAGTGGCCGACCGCCAGGCC‑TAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTa                                                                                                                                                                                             <  2:509456/246‑1 (MQ=255)
                                                        ggCTAACTAACTGGCTGGT‑GCGCCATATCTCCTGGTGATTAATGGCGGTGTGG‑ACGTTGAGAATATCCTTACGAACT‑TTGATCGCC‑ATGATGACTTCTTCCA‑GGGAACAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCC‑AGTGAGTTGCCGACCGCCAGGCC‑TAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAgg                                                                                                                                                                                           <  1:620533/250‑1 (MQ=255)
                                                                aactGGCTGGT‑GCGCCATATCTCCTGGTGATTAATGGCGGTGTGG‑GCGTTGAGAATATCCTTACGAACT‑TTTATCGCC‑ATGATGACTTCTTCCA‑GGGAACAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCC‑AAGGAGGTGCCGACCGCCAGGCCTCAAA‑CGTCGGGGGTATGGAAGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTc                                                                                                                                                                                  >  1:389803/1‑251 (MQ=255)
                                                                aacgggctggT‑GCGCCATATCTCCTGGGGATTAATGGCGGTGTGG‑ACGTTGAGAATATCCTTACGAACT‑TTGATCGCC‑ATGATGACTTCTTCCA‑GGGAACAGTTTCCGGCACGCTCGCCGATCCCGTGCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCC‑AGTGAGTTGCCGACCGCCAGGCC‑TAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGt                                                                                                                                                                                    <  1:651285/245‑1 (MQ=255)
                                                                 actGGCTGGT‑GCGCCATATCTCCTGGTGATTAATGGCGGTGTGG‑ACGTTGAGAATATCCTTACGAACT‑TTGATCGCC‑ATGATGACTTCTTCCA‑GGGAACAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCC‑AGTGAGTTGCCGACCGCCAGGCC‑TAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCa                                                                                                                                                                                 <  1:241646/251‑1 (MQ=255)
                                                                    ggctggT‑GCGCCATATCTCCTGGTGATTAATGGCGGTGTGG‑ACGTTGAGAATATCCTTACGAACT‑TTGATCGCC‑ATGATGACTTCTTCCA‑GGGAACAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCC‑AGTGAGTTGCCGACCGCCAGGCC‑TAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGttaat                                                                                                                                                                                <  1:610529/249‑4 (MQ=255)
                                                                        ggT‑GCGCCATATCTCCTGGTGATTAATGGCGGTGTGG‑GAGTTGAGAAAATCCTTACGAACT‑TTTATCGCC‑ATGATGACTTCTTCCA‑GGGAACAATTTTCGGCACGCTCGCCGATCCCCTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCC‑CGAGGGGTGCCGACCGCCAAGCC‑TCAATCGTCGTGGGTATGGACGGGAATAATGGCTTTGTCGATGTTTGGGACGCGTTCCTAccggg                                                                                                                                                                          >  1:220219/1‑247 (MQ=255)
                                                                        ggT‑GCGCCATATCTCCTGGTGATTAATGGCGGTGTGG‑ACGTTGAGAATATCCTTACGAACT‑TTGATCGCC‑ATGATGACTTCTTCCA‑GGGAACAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCC‑AGTGAGTTGCCGACCGCCAGGCC‑TAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAgg                                                                                                                                                                           <  1:101211/250‑1 (MQ=255)
                                                                          t‑GCGCCATATCTCCTGGTGATTAATGGCGGTGTGG‑ACGTTGAGAATATCCTTACGAACT‑TTGATCGCC‑ATGATGACTTCTTCCA‑GGGAACAGTTTCCGGCCCGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCC‑AGTGAGTTGCCGACCGCCAGGCC‑TAAATCGTCGTGGGGATGTACGGGAATAATGGCTTTGTCGATGTTAGGGACGCGTTCATACAAGCCg                                                                                                                                                                        >  1:818736/1‑251 (MQ=255)
                                                                             cgcCATATCTCCTGGTGATTAATGGCGGTGTGG‑ACGTTGAGAATATCCTTACGAACT‑TTGATCGCC‑ATGATGCCTTCTTCCA‑GGGAACAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCC‑AGTGAGTTGCCGACCGCCAGGCC‑TAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCt                                                                                                                                                                      <  1:342100/251‑1 (MQ=255)
                                                                                 atatCTCCTGGTGATTAATGGCGGTGTGG‑ACGTTGAGAATATCCTTACGAACT‑TTGATCGCC‑CTGATGACTTCTTCCA‑GGGAACAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCC‑CGTGAGTTGCCGACCCCCAGGCC‑TAAATCGTCGTGGGGATTTACGGGAATAATGGCTTTGTCGATGTTAGGGACGGGTTCATACAGGCCGCtggtg                                                                                                                                                                  >  1:874006/1‑248 (MQ=255)
                                                                                 atatCTCCTGGTGATTAATGGCGGTGTGG‑ACGGTGAGAATATCCTTACGAACT‑TTGATCGCG‑ATGTTGACTTCTTCCA‑GGGAACAGTGGCCGGCACGCTCGCCGATCCCGTTCATTGCGCTTTCTACCGGGCGTGCACCGGCAGGTACCGCCGCC‑AGTGAGTTGCGGACCGCCAGGCC‑TAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCtgatg                                                                                                                                                                  <  1:129762/251‑1 (MQ=255)
                                                                                      tcCTGGTGATTAATGGCGGTGTGG‑ACGTTGAGAATATCCTTACGAACC‑TTGATCGAC‑ATGTTGACCTCTTCCA‑GGGAACAGTTTCCGGCACGCCCGCCGATCACGTTTATTGCGCCTTCCCCCTGGAGTGCACCGGCCGGTACCGCCGCC‑AGTGAGTTGACGACCGCCAGGCC‑TAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTc                                                                                                                                                              <  1:546862/250‑1 (MQ=255)
                                                                                                    ggCGGTGTGG‑ACGTTGAGAATATCCTTACGAACT‑TTGATCGCC‑ATGATGACTTCTTCCA‑GGGAACAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCC‑AGTGAGTTGCCGTCCGCCAGGCC‑TAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACT‑CAAAc                                                                                                                                               <  1:580280/250‑1 (MQ=255)
                                                                                                     gCGGTGTGG‑ACGTTGAGAATATCCTTACGAACT‑TTGATCGCC‑ATGATGACTTCTTCCA‑GGGAACAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCCCCGGCATGTACCGCCGCC‑AGTGAGTTGCCGACCGCCAGGCC‑TAAAGCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACT‑CAAACgg                                                                                                                                             <  1:165691/251‑1 (MQ=255)
                                                                                                     gCGGTGTGG‑ACGTTGAGAATATCCTTACGAACT‑TTGATCGCC‑ATGATGACTTCTTCCA‑GGGAACAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCC‑AGTGAGTTGCCGACCGCCAGGCC‑TAAATCGTCGTGGGGATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATTAAGGCCGCTGATGATTCCGGCGAACT‑CAAACgg                                                                                                                                             >  2:207263/1‑251 (MQ=255)
                                                                                                        gtgtgt‑ACGTTGAGAATATCCTTACGAACT‑TTGATCGCC‑ATGATGACTTCTTCCA‑GGGAACAGTTTCCGGCCCGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCC‑AGTGAGTTGCCGACCGCCAGGAC‑ACACTCGTCGTGGGTATGTAAGGAAATAATGGCTTTGACGTTGTTAGGGACGCGTTCATACGTGCCGCTTATGGTTACGGCGGACTACAAACgcc                                                                                                                                            >  2:638138/1‑248 (MQ=255)
                                                                                                         tgtgG‑ACGTTGAGAATATCCTTACGAACT‑TTGATCGCC‑ATGATGACTTCTTCCA‑GGGAACAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCC‑AGTGAGTTGCCGACCGCCAGGCC‑TAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGGTGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACT‑CAAACGGCATg                                                                                                                                         >  2:213728/1‑251 (MQ=255)
                                                                                                           tgG‑ACGTTGAGAATATCCTTACGAACT‑TTGATCGCC‑ATGATGACTTCTTCCA‑GGGAACAGTTTCCGGCACGCTCGCCGATCCCGTTCCTTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCC‑AGTGAGTTGCCGACCGCCAGGCC‑TAAATCGGCGTGGGTATGTACGGAAATCATGGCTTTGTCGATGTTAAGGACGCGTTCATACAGGCCGCTGCAGATGCCGGCGAACT‑CAAACGGCCTGgt                                                                                                                                       >  2:382669/1‑251 (MQ=255)
                                                                                                                cGTTAAGAATATCCTTACGACCC‑TTGATCGCC‑ATGATGACTTCTTCCA‑GGGAACAGTTTCCGGACCGCTCGCCGATCCCGTACATGGCGCCTGCCACCTGGCGTGCCCCGGCATGTCCCGCCGCC‑AGTGAGTTGCCGACCGCCAGGCC‑TAAAACGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTGCGCGTTCATACAGGCCGCTGATGATTCCGGCGAACT‑CAAACGGCATGGTGTAg                                                                                                                                   <  1:581368/251‑1 (MQ=255)
                                                                                                                   gtaaaaTATCCTGACGA‑CTGTTGATCGCCTAT‑ATGCCTTCTTCCG‑GGTAACAGTTTCCGGCCCGCCCTCCGAGCCAGTCCATGTCGCCTTCCCCCTGGTGTGCCCCGTCATGTACCGCCGCC‑AGTGAGTTGCCGACCGCCAGGCC‑TAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACT‑CAAACGGCATGGTGTAGccc                                                                                                                                <  2:505209/247‑1 (MQ=255)
                                                                                                                    gagaATATCCTTACGAACT‑TTGATCGCC‑ATGATGACTTCTTCCA‑GGGAACAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCC‑AGTGAGTTGCCGACCGCCAGGCC‑TAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACT‑CAAACGGCATGGTGTAGccc                                                                                                                                <  1:567961/250‑1 (MQ=255)
                                                                                                                     agaATATCCTTACGAACT‑TTGATCGCC‑ATGATGACTTCTTCCA‑GGGAACAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCC‑AGTGAGTTGCCGACCGCCAGGCC‑TAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACT‑CAAACGGCATGGTGTAGCCCAc                                                                                                                              <  1:129838/251‑1 (MQ=255)
                                                                                                                     agaATATCCTTACGAACT‑TTGATCGCC‑ATGATGACTTCTTCCA‑GGGAACAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCC‑AGTGAGTTGCCGACCGCCAGGCC‑TAAATCGTCGTGGGTATGTAAGGAAATAATGGCTTTGTCGCTGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACG‑CAACCGGCCTGgtgtgaccccc                                                                                                                              >  2:695316/1‑244 (MQ=255)
                                                                                                                          atCCTTACGAACT‑TTGATCGCC‑ATGATGACTTCTTCCA‑GGGAACAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCC‑AGTGAGTTGCCGACCGCCAGGCC‑TAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACT‑CAAACGGCATGGTGTAGCCCACGgtgt                                                                                                                         >  1:584551/1‑251 (MQ=255)
                                                                                                                            ccTTACGAACT‑TTGATCGCC‑ATGATGACTTCTTCCA‑GGGAACAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCC‑AGTGAGTTGCCGACCGCCAGGCC‑TAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACT‑CAAACGGCATGGTGTAGCCCACGGTGTc                                                                                                                        <  1:888318/250‑1 (MQ=255)
                                                                                                                            ccTTACGAACT‑TTAATCCCC‑AGAATTCCTTCTCCCA‑GGGACCAGTTTCCGGCCCGCTCGCCTATCCCGTTCATTGCGCCTTCCCCCTGGCGTGCCCCGGCATGTCCCGCCGCC‑AGTGAGTTGCCGACCGCCAGGCC‑TAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACT‑CAAACGGCATGGTGTAGCCCACGGTGTcc                                                                                                                       <  2:589110/251‑1 (MQ=255)
                                                                                                                             cTTACGAACT‑TTGATCGCC‑ATGATGACTTCTTCCA‑GGGAACAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCC‑AGTGAGTTGCCGACCGCCAGGCC‑TAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGATCATACAGGCCGCTGATGATTCCGGCGAACT‑CAAACGGCATGGTGTAGCCCACGGGGTCCg                                                                                                                      >  1:109664/1‑251 (MQ=255)
                                                                                                                                  aaaCT‑TTGACCGCC‑ATGATTACTTCTTCCA‑GTGAACATTTTCCGGCACGCCCCCCGATCCCGTTCATTGCGCCGTCCACCTGGCGTGCACCGGCATGTACCGCCGCC‑AGTGAGTTGCCGACCGCCAGGCC‑TAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACT‑CAAACGGCATGGTGTAGCCCACGGTGTCCGGAATg                                                                                                                 <  1:65622/250‑1 (MQ=255)
                                                                                                                                     cT‑TTGATCGCC‑ATGATGACTTCTTCCA‑GGGAACAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCC‑AGTGAGTTGCCGACCGCCAGGCC‑TAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACT‑CAAACGGCATGGTGTAGCCCACGGTGTCCGGAttgttg                                                                                                              >  1:638826/1‑251 (MQ=255)
                                                                                                                                      t‑ttGATCGCC‑ATGATGACTTCTTCCA‑GGGAACAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCC‑AGTGAGTTGCCGACCGCCAGGCC‑TAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACT‑CAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTg                                                                                                              >  1:113232/1‑250 (MQ=255)
                                                                                                                                         tttgcccccagggtgtcttcttCCA‑GGGACCATTTTCCGGCACGCTCGCCGACCCCGTTCATTGCGCCTTCCACCTGGCGGGCACCGGCATGTACCGCCGCC‑AGTGAGTTGCCGACCGCCAGGCC‑TAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACT‑CAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGa                                                                                                             <  2:247489/234‑1 (MQ=255)
                                                                                                                                         tGATCGCC‑ATGATGACTTCTTCCA‑GGGAACAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCC‑AGTGAGTTGCCGACCGCCAGGCC‑TAAATCGTCGTGGGTATGCACGGAAATAAAGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGACCT‑CAAACGGCATGGTATAGCCCAAGGCGTCCGGAATGTTgttg                                                                                                           >  2:424175/1‑248 (MQ=255)
                                                                                                                                          gaccGCC‑ATGATGCCTTCTTCCA‑TGGAACAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCGCCTTGCTTGCACCGGCATGTACCGCCGCC‑AGTGAGTTGCCGACCGCCAGGCC‑TAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGTCCGCTGATGATTCCGGCGAACT‑CAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGAtg                                                                                                           <  1:965507/247‑1 (MQ=255)
                                                                                                                                           aTCGCC‑ATGATGACTTCTTCCA‑GGGAACAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCC‑AGTGAGTTGCCGACCGCCAGGCC‑TAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGGTGGTAGGTACGCGGTCATACAGGCCGCTGATGATTACGGCGAACT‑CAAACGGCATGGTGTAGACCACGGTGGCCGGGATGTTGAtggg                                                                                                         >  2:22213/1‑250 (MQ=255)
                                                                                                                                              gCC‑ATGATGACTTCTTCCA‑GGGAACAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCC‑AGTGAGTTGCCGACCGCCAGGCC‑TAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACT‑CAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGAtggtggg                                                                                                      >  1:134568/1‑250 (MQ=255)
                                                                                                                                              ccc‑ATGATGACTTCTTCCA‑GGGAACAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCC‑AGTGAGTTGCCGACCGCCAGGCC‑TAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACT‑CAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGAtggtggt                                                                                                      <  1:835937/250‑1 (MQ=255)
                                                                                                                                                ccatgtgtgcttctcCCA‑GGGAACAGTTTGCGGCACGCGCGCCGACCCCGTTCATTTCGCCTTCCACCGGGCTAGCATCGGCATGTACCGCCGCC‑AGTGAGTTGCCGACCGCCAGGCC‑TAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACT‑CAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGAtggtggtg                                                                                                     <  2:112423/242‑1 (MQ=255)
                                                                                                                                                c‑ATGATGACTTCTTCCA‑GGGAACAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCC‑AGTGAGTTGCCGACCGCCAGGCC‑TAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACT‑CAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGAtggtggtgg                                                                                                    <  1:470834/251‑1 (MQ=255)
                                                                                                                                                     atgaCTTCTTCCA‑GGGAAAAGTTTCCGGCACGCTCGCCGCTCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCC‑AGTGAGTTGCCGCCCGCCAGGCC‑GAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGGTCATACAGGCCGCTGATGATTCCGGCGAACT‑CAACCAGCATGGTGTAGACCAAGGTGTCCGGAATGTTGATGGGGGTGGCAcg                                                                                                >  2:782570/1‑250 (MQ=255)
                                                                                                                                                        aCTTCTTCCA‑GGGAACAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCAGCC‑CGTGAGTTGCCGACCGCCAGGCC‑TAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTATCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACT‑AAAACGGAATGGTGTAGCCCACGGAGTCCGGAATGTTGCTGGTGGTTGCACCGGc                                                                                             >  1:900292/1‑251 (MQ=255)
                                                                                                                                                           tcttcCA‑GGGAACAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCC‑AGTGAGTTGCCGACCGCCAGGCC‑TAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACT‑CAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCAtt                                                                                          >  1:10766/1‑251 (MQ=255)
                                                                                                                                                            cttcCA‑GGGAACAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCC‑AGTGAGTTGCCGACCGCCAGGCC‑TAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACT‑CAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCAtt                                                                                          >  1:36874/1‑250 (MQ=255)
                                                                                                                                                                                 gCACGCTCGCCGATCCCGATCATTTCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCC‑AGTGAGTTGCCGACCGCCAGGCC‑TAAATCGTAGTGGGTATGTACGGTAATAATGGCTTTGTCGCTGTTAGGTACGCGTTCATAAAGGCCGCTGATGATTCCGGCGAACT‑AAAAAGGCATGGGGTAGCACACGGTGTCCGGCATGTTTGTGGTGGTGGCACCGGCATTAATCCTCGCTTCGAACACTcc                                                                     >  2:799084/1‑250 (MQ=255)
                                                                                                                                                                                  cACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCC‑AGTGAGTTGCCGACCGCCAGGCC‑TAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACT‑CAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTcgc                                                                    >  1:304294/1‑251 (MQ=255)
                                                                                                                                                                                   aCGCTCGCCGACCCCGTTCATTGCTCCTCCCACCTGGCGGGCTCCGGCATGTACCGCCTCC‑AGTGAGTTGCCGACCGCCAGGCC‑TAAAACGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACT‑CAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTcgcg                                                                   <  1:565446/251‑1 (MQ=255)
                                                                                                                                                                                        cGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCC‑AGTGAGTTGCCGACCGCCAGGCC‑TAAATCGTCGTGGGAATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACT‑CAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCgcgca                                                                 >  1:69832/1‑247 (MQ=255)
                                                                                                                                                                                         tccGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCC‑ATTGAGTTGCCGACCGCCAGTCC‑TCAATACTCGTGGGTATGTACGGCAATAATGGCTTTGACGATGTTATGTACGCGTTCATACAGGCCGCTTATGATACCGGCGAACT‑CAAACGTCAGGGTGTAGCCACCGGTGTCAGGATTTTTAGTGGTGGAGGCACCGGCATTACTCGCATCTTCGACCCCTcgcgac                                                                 >  1:654259/2‑245 (MQ=255)
                                                                                                                                                                                         gCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCC‑AGTGAGTTGCCGACCGCCCGGCC‑TAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGTTGTTAGGTACGCGTTCCTACAGGCCGCTGATGATTCCGGCGAACT‑CAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATAGCCGCTTCGCCCACTcgcgaccgct                                                             >  1:654250/1‑245 (MQ=255)
                                                                                                                                                                                          ccgttCCCGTTCATTGCGCCGTCCACCTGGCGGGCACCGGCATGTCCAGCCGCT‑AGTGAGTTGCCGACCGCCAGGCC‑TAAAGCGTCGTGGGTATGTACGGAAAGAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGTCGCTGATGATTCCGGCGAACT‑CAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGTCCACTCGCGACAGATc                                                            <  1:547671/247‑1 (MQ=255)
                                                                                                                                                                                            gacccgtttctgtGCGCCTTCCACCTGGCGTGCCCGGGGGTGTACCGCCGCC‑AGTGAGTTGCCGACCGCCAGTCC‑TAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACT‑CAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATctg                                                          <  2:509709/239‑3 (MQ=255)
                                                                                                                                                                                            gATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCC‑AGTGAGTTGCCGACCGCCAGGCC‑TAAATCGTCGTGGGGATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACT‑CAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGCGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGcc                                                                 >  2:165511/1‑244 (MQ=255)
                                                                                                                                                                                                ccGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCC‑AGTGAGTTGCCGACCGCCAGGCC‑TAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACT‑CAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATCGGCAAt                                                      <  1:202810/251‑1 (MQ=255)
                                                                                                                                                                                                  gatCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCC‑AGTGCGTTGCCGACCGCCAGGCC‑TAAATCGTCGTGGGCATGTACGGAAATAATGACTTTGTCGCTGATAGGCACGCGTTCATACGGGCCGCTGCTGATACCGGCGAACT‑CAAACGAAATGGTGAAGCCCACGGTGTCCGGAATGTTGACGGTGGTGGCAACGGCATTACTCGCCGCTTCGACACCTCgcgcacgataggcaaagg                                                    >  1:897730/3‑237 (MQ=255)
                                                                                                                                                                                                    tCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCC‑AGTGAGTTGCCGACCGCCAGGCC‑TAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACT‑CAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACCCGCGCCAGATCGGCAATgggg                                                  >  2:504106/1‑250 (MQ=255)
                                                                                                                                                                                                     cATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCC‑AGTGAGTTGCCGACCGCCAGGCC‑TAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACT‑CAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGGGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATCGGCAATGGgtg                                                 >  1:135678/1‑251 (MQ=255)
                                                                                                                                                                                                            ccTTCCACCTGGCGTGCACCGGCATGTACCGCCGCC‑AGTGAGTTGCCGACCGCCAGGCC‑TAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACT‑CAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATCGGCAATGGGTGTACGccc                                          <  1:700776/251‑1 (MQ=255)
                                                                                                                                                                                                            ccTTCCACCTGGCGTGCACCGGCATGTACCGCCGCC‑AGTGAGTTGCCGACCGCCAGGCC‑TAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACT‑CAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATCGGCAATGGGTGTACGccc                                          <  1:778961/251‑1 (MQ=255)
                                                                                                                                                                                                              cgccccctggcttcccccGGAATGTACCGCCGCC‑AAGGAGTTGCCGACCGCCAGGCC‑TAAACCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGGTCATACAGGCCGCTGATGATTCCGGCGAACT‑CAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATCGGCAATGGGTGTACGCCCg                                         <  2:295637/234‑1 (MQ=255)
                                                                                                                                                                                                               tCCACCTGGCGTGCACCGGCATGTACCGCCGCC‑AGTGAGTTGCCGACCGCCAGGCC‑TAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACT‑CAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATCGGCAATGGGTGTACGCCCg                                         <  1:299467/249‑1 (MQ=255)
                                                                                                                                                                                                               ccccccTGGCGTGCACCGGCATGTACCGCCGCC‑AGTGAGTTGCCGACCGCCAGGCC‑TAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACT‑CAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATCGGCAATGGGTGTACGCCCgg                                        <  1:255453/246‑1 (MQ=255)
                                                                                                                                                                                                                ccgccgggCGTGCACCGGCATGTACCGCCGCC‑AGTGAGTTGCCGACCGCCAGGCC‑TAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACT‑CAAACGGCATGGTGTAGCCCGCGGTGTCCGGAATGTTGATGGTGGTGGCACCTGCATTAATCGCCGCTTCGACCACGCGCGCCAGATCGGCAATGGGTGTACGCCCGGCa                                      <  1:565160/245‑1 (MQ=255)
                                                                                                                                                                                                                 cACCTGGCGTGCACCGGCATGTACCGCCGCC‑AGTGAGTTGCCGACCGCCAGGCC‑TAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACT‑CAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATCGGCAATGGGTGTACGCCCGGCAt                                     >  1:365108/1‑251 (MQ=255)
                                                                                                                                                                                                                   cagggCGTGAACCGTGATGTACCGTCGCC‑AGTGAGTTGCCGACCTCCAGGAC‑TAAATCGGCGTGGGTATGTACGGAAATAAGGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACT‑CAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATCGGCAATGGGTGTACGCCCGGCATCt                                   <  2:870563/248‑1 (MQ=255)
                                                                                                                                                                                                                    cgggCGTGCACCGGCATGTACCGCCGCC‑AGTGAGTTGCCGACCGCCAGGCC‑TAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACT‑CAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATCGGCAATGGGTGTACGCCCGGCATCtt                                  <  1:382669/249‑1 (MQ=255)
                                                                                                                                                                                                                     gggCGTGCACCGTCTGGTACCGCCGCC‑AGTGGGTTGCCGACCGCCAGGCC‑TAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACT‑CAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATCGGCAATGGGTGTACGCCCGGCATCTTc                                 <  2:602309/250‑1 (MQ=255)
                                                                                                                                                                                                                     cggCGTGCACCGGCATGTACCGCCGCC‑AGTGAGTTGCCGACCGCCAGGCC‑TAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACT‑CAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATCGGCAATGGGTGTACGCCCGGCATCTTc                                 <  2:233728/250‑1 (MQ=255)
                                                                                                                                                                                                                           gcatcgccATGTACCGCCGCC‑AGTGAGTGGCCGACCGCCAGGCC‑TAAATCGTCGTTGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACT‑CAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCTCTTCGACCACTCGCGCCAGATCGGCAATGGGTGTACGCCCGGCATCTTCGCAAg                            <  2:789897/243‑1 (MQ=255)
                                                                                                                                                                                                                           gCACCGGCATGTACCGCCGCC‑AGTGAGTTGCCGACCGCCAGGCC‑TAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACT‑CAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGCCCACTCGCGCCAGTTCGGCAATGGGTGTACGCCCGGCATCTTCGCa                              >  1:29740/1‑248 (MQ=255)
                                                                                                                                                                                                                            cACCGGCATGTACCGCCGCC‑AGTGAGTTGCCGACCGCCAGGCC‑TAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACT‑CAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATCGGCAATGGGTGTACGCCCGGCATCTTCGCAAGaa                          <  1:459528/251‑1 (MQ=255)
                                                                                                                                                                                                                             aCCGGCATGTACCGCCGCC‑AGTGAGTTGCCGACCGCCAGGCC‑TACATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACT‑CAAACGGCATGGTGTCGCCCACGGTGTCCGGGATGTTGATGGTGGGGGCACCGGCATTAATCGCCGCTTCGACCACCCGCGCCAGAACGGCAATGGGGGTACGCCCGGCATCCTCGCAAGaa                          >  2:977034/1‑250 (MQ=255)
                                                                                                                                                                                                                             aCCGGCATGTACCGCCGCC‑AGTGAGATTCCGACCGACAGGCC‑TAAAACGTCGAGGGTATTTACGGAAATAATGGCTTTGTCGATGTTAGGGACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACA‑CAAACGGCATGGTGTAGCCCACGGTGACCGGAATGTTGATGGGGGTGGCACCGGCACTCATCGACGCTTCGACCACTCGCGCCAGATCCGCCATGGGTGAACACCCGGccacttcggaagga                          >  2:137760/1‑237 (MQ=255)
                                                                                                                                                                                                                              ccGGCATGTACCGCCGCC‑AGTGAGTTGCCGACCGCCAGGCC‑TAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACT‑CAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATCGGCAATGGGTGTACGCCCGGCATCTTCGCAAGaaa                         >  1:773805/1‑250 (MQ=255)
                                                                                                                                                                                                                                 gcaggTACCGCCGCC‑AGTGAGTTGCCGACCGCCAGGCC‑TAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACT‑CAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATCGGCAATGGGTGTACGCCCGGCATCTTCGCAAGAAAATTc                     <  2:874006/247‑1 (MQ=255)
                                                                                                                                                                                                                                  cATGTACCGCCGCC‑AGTGAGTTGCCGACCGCCAGGCC‑TAAATCGTCGTGGGTATGTAAGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACT‑CAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCCCTCGCGCCAGATCGGCAATGGGTGTACGCCCGGCATCTTCGAAAGACAATTc                     >  2:150686/1‑250 (MQ=255)
                                                                                                                                                                                                                                      tACCGCCGCC‑AGTGGGTTGCCGACCGCCGGGCC‑TGAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACT‑CAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATCGGCAATGGGTGTACGCCCGGCATCTTCGCAAGAAAATTCAAcat                >  1:223188/1‑251 (MQ=255)
                                                                                                                                                                                                                                          cccgcc‑AGTGAGTTGCCGACCGCCAGGCC‑TAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACT‑CAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATCGGCAATGGGTGTACGCCCGGCATCTTCGCAAGAAAATTCAACatcatc            <  2:226592/250‑1 (MQ=255)
                                                                                                                                                                                                                                            cgcc‑AGTGAGTTGCCGACCGCCAGGCC‑TAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACT‑CAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATCGGCAATGGGTGTACGCCCGGCATCTTc                                 <  1:274495/228‑1 (MQ=255)
                                                                                                                                                                                                                                              cc‑AGTGAGTTGCCGACCGCCAGGCC‑TAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACT‑CAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATCGGCAATGGGTGTACGCCCGGCATCTTCGCAAGAAAATTCAACATCATCGgtg        <  1:439255/251‑1 (MQ=255)
                                                                                                                                                                                                                                              cc‑AGTGAGTTGCCGACCGCCAGGCC‑TAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACT‑CAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATCGGCAATGGGGGTACGCCCGGCCTCTTCGCAAGAAAATTCAACTTCCTCGgtg        >  2:883312/1‑251 (MQ=255)
                                                                                                                                                                                                                                                 aGTGAGTTGCCGACCGCCAGGCC‑TAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACT‑CAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATCGGCAATGGGTGTACGCCCGGCATCTTCGCAAGAAAATTCAACATCATCGGTGTa      <  1:674821/251‑1 (MQ=255)
                                                                                                                                                                                                                                                     aGTTGCCGACCGCCAGGCC‑TAAATCGTCGTGGGTATATACGGAAATAATGGCTTTGTCGATGTTAGGTACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACT‑CAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATCGGCAATGGGTGTACGCCCGGCATCTTCGCAAGAAAATTCAACATCATCGGTGTAATTa  <  1:936111/251‑1 (MQ=255)
                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                        
GAATGCGCCGCTGCCAACAATGGCTTTGTTTGCCGGGATCGGCATATTACAAATCTGGCTAACTAACTGGCTGGT‑GCGCCATATCTCCTGGTGATTAATGGCGGTGTGG‑ACGTTGAGAATATCCTTACGAACT‑TTGATCGCC‑ATGATGACTTCTTCCA‑GGGAACAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGCACCGGCATGTACCGCCGCC‑AGTGAGTTTCCGACCGCCAGGCC‑CAAATCGTCGTGGGTATGTACGGAGATAATGGCTTTGTCGATGTTAGGCACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACT‑CAAACGGCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATCGGCAATGGGTGTACGCCCGGCATCTTCGCAAGAAAATTCAACATCATCGGTGTAATTA  >  NC_000913/82651‑83140

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑