Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 80,529 C→G G112G (GGG→GGC)  leuC ← 3‑isopropylmalate dehydratase subunit LeuC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091380,5290CG99.0% 315.1 / NA 105G112G (GGG→GGC) leuC3‑isopropylmalate dehydratase subunit LeuC
Reads supporting (aligned to +/- strand):  ref base C (0/1);  major base G (51/51);  minor base T (0/1);  total (52/53)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.46e-01

Only 100 of 107 total aligned reads displayed.

CTGCGCTACCGGTTTTACCGATAATTGCCAGCACGATATCTTTTGCGGTAATGCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTCATGGTTTTTGCGCGGCCCTGT‑TTCAGGGTTTGCGTTGCCAGTACGTGTTCAACTTCGGAAGTGCCGATACCAAAGGCCAGTGCGCCAAACGCGCCGTG‑GGTGGCGGTATGCGAGTCGCCGCAGACA‑ATGGTCATCCCCGGCAAGGTGACGCCCTGTTCCGGCCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGACGTTGTGATCCATGGTAGCGAAGGTTTTGCCCGGCTGACGTACCGGGCGACCGTGGGCGCGCAGACCATCGAACGCCTGCGGTGAGGTCACTTCATG  >  NC_000913/80282‑80768
                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                               
 tGCGCTACAGGTTTTACCGATAATTGCCAGCACGATATCTTTTGCGGTAATGCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTCATGGTTTTTGCGCGGCCCTGT‑TTCAGGGTTTGCGTTGCCAGTACGTGTTCAACTTCGGAAGTGCCGATACCAAAGGCCAGTGCGCCAAACGCGCCGTG‑GGTGGCGGTATGCGAGTCGCCGCCGACA‑ATGGTCATCCCCGGCAAGGTGACGCCCTGTTCCGGgcccc                                                                                                                                                                                                                                             >  2:879006/1‑250 (MQ=255)
  gcgatcccGGTTTTACCGATAATTGCCTGCACGATATCTTTTGCGGTAATGCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTCATGGTTTTTGCGCGGCCCTGT‑TTCAGGGTTTGCGTTGCCAGTTCGTGTTCAACTTCGGAAGTGCCGATACCAAAGGCCAGTGCGCCAAACGCGCCGTG‑GGTGGCGGTATGCGAGTCGCCGCAGACA‑ATGGTCATCCCCGGCAAGGTGACGCCCTGTTCCGGGCCCAt                                                                                                                                                                                                                                            <  2:655959/245‑1 (MQ=255)
    gcTACCGGTTTTACCGGTAATTGCCAGCACGATATCTTTTGCGGTAATGCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTCATGGTTTTTGCGCGGCCCTGT‑TTCAGGTTTTGCGTTGCCAGTACGTGTTCAACTTCGGAAGTGCCGATCCCAAAGGCCAGTGCGCCAAACGAGCCGTG‑GGTGGCGGTAAGGGAGTCGACGCAGACA‑ATGGTCATCCCCGGCAAGGTGACGCCCTGTTCCGGGCCCaatc                                                                                                                                                                                                                                          >  2:768170/1‑248 (MQ=255)
     cTACCGGTTTTACCGATAATTGCCAGCACGATATCTTTTGCGGTAATGCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTCATGGTTTTTGCGCGGCCCTGT‑TTCAGGGTTTGCGTTGCCAGTACGTGTTCAACTTCGGACGTGCCGATACCAAAGGCCAGTGCGCCAAACGCGCCGTG‑GGTGGCGGTATGCGAGTCGCCGCCGACA‑ATGGTCATCCCCGGCAAGGTGACGCCCTGTTCAGGGCCCCTTAc                                                                                                                                                                                                                                         >  2:641530/1‑251 (MQ=255)
      tACCGGTTTTACCGATAATTGCCAGCACGATATCTTTTGCGGTAATGCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTCATGGTTTTTGCGCGGCCCTGT‑TTCAGGGTTTGCGTTGCCAGTACGTGTTCAACTTCGGAAGTGCCGATACCAAAGGCCAGTGCGCCAAACGCGCCGTG‑GGTGGCGGTATGCGAGTCGCCGCAGACA‑ATGGTCATCCCCGGCAAGGTGACGCCCTGTTCCGGGCCCATTACg                                                                                                                                                                                                                                        >  1:464643/1‑251 (MQ=255)
       aCCGGTTTTACCGATAATTGCCAGCACGATATCTTTTGCGGTAATGCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTCATGGTTTTTGCGCGGCCCTGT‑TTCAGGGTTTGCGTTGCCAGTACGTGTTCAACTTCGGAAGTGCCGATACCAAAGGCCAGTGCGCCAAACGCGCCGTG‑GGTGGCGGTATGCGAGTCGCCGCAGACA‑ATGGTCATCCCCGGCAAGGTGACGCCCTGTTCCGGGCCCATTACGt                                                                                                                                                                                                                                       >  1:788710/1‑251 (MQ=255)
         cGGTTTTACCGATAATTGCCAGCACGATATCTTTTGCGGTAATGCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTCATGGTTTTTGCGCGGCCCTGT‑TTCAGGGTTTGCGTTGCCAGTACGTGTTCAACTTCGGAAGTGCCGATACCAAAGGCCAGTGCGCCAAACGCGCCGTG‑GGTGGCGGTATGCGAGTCGCCGCAGACA‑ATGGTCATCCCCGGCAAGGTGACGCCCTGTTCCGGGCCCATTACGTgg                                                                                                                                                                                                                                     <  2:158750/251‑1 (MQ=255)
          ggTTTTACCGATAATTGCCAGCACGATATCTTTTGCGGTAAGGCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTCATGGTTTTTGCGCGGCCCTGT‑TTCAGGGTTTGCGTTGCCAGTACGTGTTCAACTGCGGAAGTGCCGATACCAAAGGCCAGTGCGCCAAACGCGCCGTG‑GGTGGCGGTATGCGAGTCGCCGCAGACA‑ATGGTCATCCCCGGCAAGGTGACGCCCTGTTCCGGGCCCATTACGTGGa                                                                                                                                                                                                                                    <  2:533550/251‑1 (MQ=255)
           gTTTTACCGATAATTGCCAGCACGATATCTTTTGCGGTAATGCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTCATGGTTTTTGCGCGGCCCTGT‑TTCAGGGTTTGCGTTGCCAGTACGTGTTCAACTTCGGAAGTGCCGATACCAAAGGCCAGTGCGCCAAACGCGCCGTG‑GGTGGCGGTATGCGAGTCGCCACAGACA‑ATGGTCATCCCCGGCAAGGGGACGCCCTGTTACGGGCCCCTTACGTGGAc                                                                                                                                                                                                                                   >  2:233765/1‑251 (MQ=255)
             tttACCGATAATTGTCAGCACGATATCTTTTGCGTTAATGCCCGGCGCGGCTTTGCTCTGGACTTCAATTTTCATGGTTTTTGCGCGGCCCTGT‑TTCAGGGTTTGCGGTGCCAGTACGTGTTCAACTTCGGAAGGGCCGATACCAAAGGCCAGTGCGCCAAACGAGCCGTG‑GGTGGCGGTATGCGAGTCGCCGCAGACA‑ATGGTCATCCCCGGCAAGGTGACGCCCTGTTCCGGGCCCATTACGTGGACg                                                                                                                                                                                                                                  <  2:885510/250‑1 (MQ=255)
                   gATAATTGCCAGCACGATATCTTTTGCGGTAATGCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTCATGGTTTTTGCGCGGCCCTGT‑TTCAGGGTTTGCGTTGCCAGTACGTGTTCAACTTCGGAAGTGCCGATACCAAAGGCCAGTGCGCCAAACGCGCCGTG‑GGTGGCGGTATGCGAGTCGCCGCAGACA‑ATGGTCATCCCCGGCAAGGTGACGCCCTGTTCCGGGCCCATTACGTGGGCGCTCCCCt                                                                                                                                                                                                                           >  2:955197/1‑251 (MQ=255)
                       aTTGTCAGCTCGATATCTTTTGCGGTAATGTCCGGCGCGGCTTTGCCCTGGACTTCAATTTTCATGGTTTTTTCGCGGCCCTGT‑TTCAGGGTTTGCGTTGCCAGTACGTGTTCAACTTCGGATGTGCCGATACCAAAGTCCAGTGCGCCAAACGCGCCGTG‑GGTGGTGGTATGCGAGTCGCCGCAGACA‑ATGGTCAGCCCCGGCAAGGTGACGCCCTGTTCCGGTCCCATTTCGTGGACGTTCCCCTGATa                                                                                                                                                                                                                       <  2:147069/251‑1 (MQ=255)
                        ttGCCAGCACGATATCTTTTGCGGTAATGCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTCATGGTTTTTGCGCGGCCCTGT‑TTCAGGGTTTGCGTTGCCAGTACGTGTTCAACTTCGGAAGTGCCGATACCAAAGGCCAGTGCGCCAAACGCGCCGTG‑GGTGGCGGTATGCGAGTCGCCGCAGACA‑ATGGTCATCCCCGGCAAGGTGACGCCCTGTTCCGGGCCCATTACGTGGACGATCCCCTGATAc                                                                                                                                                                                                                      >  1:483859/1‑251 (MQ=255)
                          gCCAGCACGATATCTTTTGCGGTAATGCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTCATGGTTTTTGCGCGGCCCTGT‑TTCAGGGTTTGCGTTGCCAGTACGTGTTCAACTTCGGAAGTGCCGATACCAAAGGCCAGTGCGCCAAACGCGCCGTG‑GGTGGCGGTATGCGAGTCGCCGCAGACA‑ATGGTCATCCCCGGCAAGGTGACGCCCTGTTCCGGGCCCATTACGTGGACGATCCCCTGATACgg                                                                                                                                                                                                                    >  1:353299/1‑251 (MQ=255)
                          gCCAGCACGATATCTTTTGCGGTAATGCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTCATGGTTTTTGCGCGGCCCTGT‑TTCAGGGTTTGCGTTGCCAGTACGTGTTCAACTTCGGAAGTGCCGATACCAAAGGCCAGTGCGCCAAACGCGCCGTG‑GGTGGCGGTATGCGAGTCGCCGCAGACA‑ATGGTCATCCCCGGCAAGGTGACGCCCTGTTCCGGGCCCATTACGTGGACGATCCCCTGATACg                                                                                                                                                                                                                     <  1:232347/250‑1 (MQ=255)
                                  gATATCTTTTGCGGTAATGCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTCATGGTTTTTGCGCGGCCCTGT‑TTCAGGGTTTGCGTTGCCAGTACGTGTTCAACTTCGGAAGTGCCGATACCAAAGGCCAGTGCGCCAAACGCGCCGTG‑GGTGGCGGTATGCGAGTCGCCGCAGACA‑ATGGTCATCCCCGGCAAGGGGACGCCCTGTTCCGGGCCCATTACGTGGACGATCCCCTGATACGGGTGCTTcg                                                                                                                                                                                                            >  2:597492/1‑250 (MQ=255)
                                   atatTTTTTGCGGTAATGCCCGGCGCGGCTTTGCGCTGGACGTCAATGTTCATGGGTTTGGCGCGGCCCTGG‑TTCAGGGTTTGCGTTTCCAGTAAGGGGTCAACTTCGGAAGTGCCGCGACCAAAGGCAAGAGCGCCAAACGCGCCGTG‑GGTGGCGGTATGCGAGTCGCCGCAGACA‑ATTGTCATCCCCGTCAAGGTGAGGCCCTGTTACGGGCCCCTTACGTGGACGTGCCCCTGATACGGGTGaatc                                                                                                                                                                                                             <  2:83679/249‑4 (MQ=255)
                                   atatCTTTTGCGGTAATGCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTCATGGTTTTTGCGCGGCCCTGT‑TTCAGGGTTTGCGTTGCCAGTACGTGTTCAACTTCGGAAGTGCCGATACCAAAGGCCAGTGCGCCAAACGCGCCGTG‑GGTGGCGGTATGCGAGTCGCCGCAGACA‑ATGGTCATCCCCGGCAAGGTGACGCCCTGTTCCGGGCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAg                                                                                                                                                                                                           <  1:441202/251‑1 (MQ=255)
                                   atatCTTTTGCGGTAATGCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTCATGGTTTTTGCGCGGCCCTGT‑TTCAGGGTTTGCGTTGCCAGTACGTGTTCAACTTCGGAAGTGCCGATACCAAAGGCCAGTGCGCCAAACGCGCCGTG‑GGTGGCGGTATGCGAGTCGCCGCAGACA‑ATGGTCATCCCAGGCAAGGTGACGCCCTGTTCCGGGCCCATTACGTGGACGATCCCCTGATACGGGGGATTc                                                                                                                                                                                                             >  1:541940/1‑249 (MQ=255)
                                     atCTTTTGCGGTAATGCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTCATGGTTTTTGCGCGGCCCTGT‑TTCAGGGTTTGCGTTGCCAGTACGTGTTCAACTTCGGAAGTGCCGATACCAAAGGCCAGTGCGCCAAACGCGCCGTG‑GGTGGCGGTATGCGAGTCGCCGCAGACA‑ATGGTCATCCCCGGCAAGGTGACGCCCTGTTCCGGGCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGt                                                                                                                                                                                                         >  1:558605/1‑251 (MQ=255)
                                         tttGCGGTAATGCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTCATGGTTTTTGCGCGGCCCTGT‑TTCAGGGTTTGCGTTGCCAGTACGTGTTCAACTTCGGAAGTGCCGATACCAAAGGCCAGTGCGCCAAACGCGCCGTG‑GGTGGCGGTATGCGAGTCGCCGCAGACA‑ATGGTCATCCCCGGCAAGGTGACGCCCTGTTCCGGGCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCAt                                                                                                                                                                                                      <  1:388463/250‑1 (MQ=255)
                                                 caTGCCCGGCGTGGCTTTGCCCTGGACTTCAATTTTCATGGTTTTTGCGCGGCCCTGT‑TTCAGGGTTTGCGTTGCCAGTACGTGTTCAACTTCGGAAGTGCCTATACCAAAGGCCAGTGCGCCAAACGCGCCGTG‑GGTGGCGGTATGCGAGTCGCCGCAGACA‑ATGGTCATCCCCGGCAAGGTGACGCCCTGTTCCGGGCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCg                                                                                                                                                                                              <  2:723021/249‑1 (MQ=255)
                                                 aaTGCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTCATGGTTTTTGCGCGGCCCTGT‑TTCAGGGTTTGCGTTGCCAGTACGTGTTCAACTTCGGAAGTGCCGATACCAAAGGCCAGTGCGCCAAACGCGCCGTG‑GGTGGCGGTATGCGAGTCGCCGCAGACA‑ATGGTCATCCACGGCAAGGTGACGCCCTGTTCCGGGCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCg                                                                                                                                                                                              >  1:556037/1‑250 (MQ=255)
                                                  aTGCCCGGTGCGGCTTTGCACTGGCCTGCAATTTTCATGGTGTTGGCGCGGCCCTGG‑TTCAGGGGTTGCGTTGCCAGTACGTGTGCAACTTCGGAAGTGCCGATACCAAAGGCCAGTGCGCCAAACGCGCCGTG‑GGTGGCGGTATGCGAGTCTCCGCAGACA‑ATGGTCATCCCCGGCAAGGTGTCGCCCTGTTCCGGGCCCATTACGTGGACGATCCCCTGATCCGGGTGATTCATGTCATACAGTTctac                                                                                                                                                                                            <  2:847246/251‑4 (MQ=255)
                                                      ccGGTGCGGCTTTGCCCTGGACTTCAATTTTCATGGTTTTTGCGCGGCCCTGT‑TTCAGGGTTTGCGTTGCCAGTACGTGTTCAACTTCGGAAGTGCCGATACCAAAGGCCAGTGCGCCAAACGCGCCGTG‑GGTGGCGGTATGCGAGTCGCCGCAGACA‑ATGGTCATCCCCGGCAAGGTGACGCCCTGTTCCGGGTCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCa                                                                                                                                                                                        <  2:573969/251‑1 (MQ=255)
                                                      ccGGCGCGGCTTTGCCCTGGACTTCAATTTTCATGGTTTTTGCGCGGCCCTGT‑TTCAGGGTTTGCGTTGCCAGTACGTGTTCAACTTCGGAAGTGCCGATACCAAAGGCCAGTGCGCCAAACGCGCCGTG‑GGTGGCGGTATGCGAGTCGCCGCAGACA‑ATGGTCATCCCCGGCAAGGTGACGCCCTGTTCCGGGCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCa                                                                                                                                                                                        <  1:703837/251‑1 (MQ=255)
                                                        ggCGTGGCTGTGCCCTGGGCGGCAATTGTCATGGTTGTTGCGCGGACCTGT‑GTCAGGGTTTGCGGTGCCAGTACGTGTTCAACTTTGGAAGTGCCGATACCAAAGGCCAGTGCGCCAAACGCGCCGTG‑GGTGGCGGTATGCGAGTCGCCGCAGACA‑AAGGGCATCCCCGGCAAGGTGACGCCCTGTTATGGGCCCATTACGTGGACGATCCCCTGAGACGGGTGATTCAGGTCATACAGTTCGACGCCaaa                                                                                                                                                                                      <  1:145472/251‑1 (MQ=255)
                                                           gcggtgttGCCCTGGACTTCAATTTTCATGGTGTTGGCGCGGCCCTGT‑TTCAGGGTTTGCGTTGCCAGTACGTGTTCAACGTCGGAAGTGCCGATACCAAAGGCCAGTGCGCCAAACGCGCCGTG‑GGTGGCGGTATGCGAGTCGCCGCAGACA‑ATGGTCATCCCCGGCAAGGTGACGCCCTGTTCCGGGCCCATTACGTGGACGATCCCCTGATACGGGTGATTCATGTCATACAGTTCGTCGCCAAATTc                                                                                                                                                                                   <  2:944896/245‑1 (MQ=255)
                                                             ggCTTTGCCCTGGACTTCAATTTTCATGGTTTTTGCGCGGCCCTGT‑TTCAGGGTTTGCGTTGCCAGTACGTGTTCAACTTCGGAAGTGCCGATACCAAAGGCCAGTGCGCCAAACGCGCCGTG‑GGTGGCGGTATGCGAGTCGCCGCAGACA‑ATGGTCATCCCCGGCAAGGTGACGCCCTGTTCCGGGCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCtt                                                                                                                                                                                 <  2:831430/251‑1 (MQ=255)
                                                                     ccTGGACTTCAATTTTCATGGTTTTTGCGCGGCCCTGT‑TTCAGGGTTTGCGTTGCCAGTACGTGTTCAACTTCGGAAGTGCCGATACCAAAGGCCAGTGCGCCAAACGCGCCGTG‑GGTGGCGGTATGCGAGTCGCCGCAGACA‑ATGGTCATCCCCGGCAAGGTGACGCCCTGTTCCGGGCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGttt                                                                                                                                                                         >  1:738462/1‑251 (MQ=255)
                                                                      cTGGACTTCAATTTTCATGGTTTTTGCGCGGCCATGT‑TTCAGGGGTTGAGTTGCCAGTACGTGTTCAAATTCGAAGGTGCCGATGCCAAAGGGAAGTGCGGCAAACGAGGCGTG‑GATGGGGGGATGCGAGTCGCCGCAGACA‑ATGGTCCTCACCGGCAAGATGAGGCACTGGTCCGGACCCATTACGTGGAAGATGCGCTGATACGGGTGATTGAGGTCAGAAAGGTAGACGCAAAATTCTTTGCAGgttt                                                                                                                                                                        >  1:548051/1‑251 (MQ=255)
                                                                       tGGACTTCAATTTTCATGGTTTTTGCGCGGCCCTGT‑TTCAGGGTTTGCGTTGCCAGTACGTGTTCAACTTCGGAAGTGCCGATACCAAAGGACAGTGCGCCAAACGCGCCGTG‑GGTGGCGGTATGCGAGTCGCCGCAGACA‑CTGGTCATCCCCGGCAAGGTGACGCCCTGTTCCGGGACCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGttttt                                                                                                                                                                       >  1:623254/1‑251 (MQ=255)
                                                                        ggACTTCAATTTTCATGGTTTTTGCGCGGCCCTGT‑TTCAGGGTTTGCGTTGCCAGTACGTGTTCAACTTCGGAAGTGCCGATACCAAAGGCCAGTGCGCCAAACGCGCCGTG‑GGTGGCGGTATGCGAGTCGCCGCAGACA‑ATGGTCATCCCCGGCAAGGTGACGCCCTGTTCCGGGCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGttttt                                                                                                                                                                       <  1:713614/250‑1 (MQ=255)
                                                                          aCTTCAATTTTCATGGTTTTTGCGCGGCCCTGT‑TTCAGGGTTTGCGTTGCCAGTACGTGTTCAACTTCGGAAGTGCCGATACCAAAGGCCAGTGCGCCAAACGCGCCGTG‑GGTGGCGGTATGCGAGTCGCCGCAGACA‑ATGGTCATCCCCGGCAAGGTGACGCCCTGTTCCGGGCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTGAt                                                                                                                                                                    <  1:917943/251‑1 (MQ=255)
                                                                             tCAATTTTCATGGTTTTTGCGCGGCCCTGT‑TTCAGGGTTTGCGTTGCCAGTACGTGTTCAACTTCGGAAGTGCCGATACCAAAGGCCAGTGCGCCAAACGCGCCGTG‑GGTGGCGGTATGCGAGTCGCCGCAGACA‑ATGGTCATCCCCGGCAAGGTGACGCCCTGTTCCGGGCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTGATCa                                                                                                                                                                  <  1:491049/250‑1 (MQ=255)
                                                                             tCAATTTTCATGGTTTTTGCGCGGCCCTGT‑TTCAGGGTTTGCGTTGCCAGTACGTGTTCAACTTCGGAAGTGCCGATACCAAAGGCCAGTGCGCCAAACGCGCCGTG‑GGTGGCGGTATGCGAGTCGCCGCAGACA‑ATGGTCATCCCCGGCAAGGTGACGCCCTGTTCCGGGCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTGATCa                                                                                                                                                                  <  1:48008/250‑1 (MQ=255)
                                                                              cAATTTTCGTGGTGTTTGCGCGGACCTGT‑TTCAGGTTTTGCGTTGCCAGGACGTGTTCAACTTTGTAAGTGCCGATACCAAAGGCCAGGTCGCCAAACGAGCCGTG‑GGTGGCGGTATGCGGGTCGCCGCAGACA‑ATGGTCATCCCCGGCAAGGTGGCGCACTGTTCCGGGCCCATTGCGTGGAGTATCTGCTTATGCGGGTGATTGAGGTCATACAGTTCGACGTCAAATTCTGTGCGGGGTTTGATCAGt                                                                                                                                                                <  2:115745/251‑1 (MQ=255)
                                                                              cAATTTTCATGGTTTTTGCGCGGCCCTGT‑TTCAGGGGTTGCGTTGCCAGTACGTGTTCAACTTCGGAAGTGCCGATACCAAAGGCCAGTGCGCCAAACGCGCCGTG‑GGTGGCGGTATGCGAGTCGCCGCAGACA‑ATGGTCATCCCCGGCAAGGTGACGCCCTGTTCCGGGCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTGATCAGt                                                                                                                                                                <  1:9194/251‑1 (MQ=255)
                                                                                 ttttCATGGTTTTTGAGCGGCCCTGT‑TTCAGGGTTTGCGTTGCCAGTACGTGTTCAACTTCGGAAGTGCCGATACCAAAGGCCAGTGCGCCAAACGCGCCGTG‑GGTGGCGGTATGCGAGTCGCCGCAGACA‑ATGGTCATCCCCGGCAAGGAGACGCCCTGTTCCGGGCCCATTACGTGGACGATCCCATGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTGATCAGTTcc                                                                                                                                                             >  1:249381/1‑251 (MQ=255)
                                                                                  tttCATGGTTTTTGCGCGGCCCTGT‑TTCAGGGTTTGCGTTGCCAGTACGTGTTCAACTTCGGCCGTGCCGATACCAAAGGCCAGTGCGCCAAACGCGCCGTG‑GGTGGCGGTATGCGAGTCGCCGCAGACA‑ATGGTCATCCCCGGCAAGGTGACGCCCTGTTCCGGGCCCATTACGTGTACGATCCCCTGATACGGGTGATTCCGGTCATACAGTTCGACGCCAAATTCTTTGCTGTTTTTGATCAATTCCt                                                                                                                                                            >  2:454960/1‑251 (MQ=255)
                                                                                   ttCATGGTTTTTGCGCGGCCCTGT‑GTCAGGGTTTGCGTTGCCAGTACGTGTTCAACTTCGGAAGTGCCGATACCAAAGGCCAGTGCGCCAAACGCGCCGTG‑GGTGGCGGTATGCGAGTCGCCGCAGACA‑ATGGTCATCCCCGGCAAGGTGACGCCCTGTTCCGGGCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTGATCAGTTCCt                                                                                                                                                            <  1:192900/250‑1 (MQ=255)
                                                                                     cATGGTTTTTGCGCGGCCCTGT‑TTCAGGGTTTGCGTTGCCAGTACGTGTTCACCTTCGGACGTGCCGATACCAAAGGCCAGTGCGCCAAACGCGCCGTG‑GGTGGCGGTATGCGAGTCGCCGCAGACA‑ATGGTCATCCCCGGCAAGGTGACGCCCTGTTCCGGGCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGTCGCCACATTCTTTGCAGTTTTTGATTAGTTCCttc                                                                                                                                                          >  2:330820/1‑248 (MQ=255)
                                                                                       tGGTTTTTGCGCGGCCCTGT‑TTCAGGGTTTGCGTTGCCAGTACGTGTTCAACTTCGGAAGTGCCGATACCAAAGGCCAGTGCGCCAAACGCGCCGTG‑GGTGGCGGTATGCGAGTCGCCGCAGACA‑ATGGTCATCCCCGGCAAGGTGACGCCCTGTTCCGGGCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTGATCAGTTCCTGCATc                                                                                                                                                       >  2:971841/1‑251 (MQ=255)
                                                                                         gTTTTTGCGCGGCCCTGT‑TTCAGGGTTTGCGTTGCCAGTACGTGTTCAACTTCGGAAGTGCCGATACCAAAGGCCAGTGCGCCAAACGCGCCGTG‑GGTGGCGGTATGCGAGTCGCCGCAGACA‑ATGGTCATCCCCGGCAAGGTGACGCCCTGTTCCGGGCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTGATCAGTTCCTGCATCTg                                                                                                                                                     >  2:314250/1‑251 (MQ=255)
                                                                                            gttGCGCGGCCC‑GTGTTCAGGGTTTGCGGTGCCAGTACGTGTTCAACTTCGGAAGTGCCGATACCAAAGGCCAGTGCGCCAAACGCGCCGTG‑GGTGGCGGTATGCGAGGCGCCGCAGACA‑ATGGTCATCCCCGGCAAGGTGACGCCCTGTTCCGGGCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTGATCAGTTCCTGCATCTGGa                                                                                                                                                   <  2:464643/249‑1 (MQ=255)
                                                                                              tGCGCGGCCCTGT‑TTCAGGGTTTGCGTTGCCAGTACGTGTTCAACTTCGGAAGTGCCGATACCAAAGGCCAGTGCGCCAAACGCGCCGTG‑GGTGGCGGTATGCGAGTCGCCGCAGACA‑ATGGTCATCCCCGGCAAGGTGACGCCCTGTTCCGGGCCCATTCCGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTGATCAGTTCCTGCATCTGGATAc                                                                                                                                                >  2:987538/1‑251 (MQ=255)
                                                                                              tGCGCGGCCCTGT‑TTCAGGGTTTGCGTTGCCAGTACGTGTTCAACTTCGGAAGTGCCGATACCAAAGGCCAGTGCGCCAAACGCGCCGTG‑GGTGGCGGTATGCGAGTCGCCGCAGACA‑ATGGTCATCCCCGGCAAGGTGACGCCCTGTTCCGGGCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTGATCAGTTCCTGCATCTGGATAc                                                                                                                                                >  1:875847/1‑251 (MQ=255)
                                                                                              tGCGCGGACATGT‑TTCAGGGTTTGCGTTGCCAGTACGTGTTCAACTTCGGAGGTGCCGATACCAAAGGCCCGTGCGCCAACCGCGCCGTG‑GGTGGCGGTATGCGAGTCGCCGCAGACA‑ATGGTCATCCCCGGCAAGGCGACGCCCTGCTCCGGGCCCATTACGTGGCAGATCCCCTGATACGGGAGATTCAGGTCATACCGGTCGCCGCCCAATTCTTTGCCGTTTTTGATCAGTTCCTGCATCTGGaaa                                                                                                                                                 >  2:642904/1‑248 (MQ=255)
                                                                                               gcgcGGTCCTGT‑TTCATGGTTTGCGGTGCCAGTACGTGTTCAACTTCGGAAGGGCCGATACCAAAGTCCATTGCGCCAAACGCGCCGTG‑GTTGGCGGTATGCGAGTCGCCGCAGACA‑AGGGTCTTCCCCGGCAAGGTGACGCCCTGTTCCGGGCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTGACCAGTTCctggatctggat                                                                                                                                                  <  2:840166/248‑1 (MQ=255)
                                                                                               gcgcGGCCCTGT‑TTCAGGGTTTGCGTTGCCAGTACGTGTTCAACTTCGGAAGTGCCGATACCAAAGGCCAGTGCGCCAAACGCGCCGTG‑GGTGGTGGTATGCGAGTCGCCGCAGACA‑ATGGTCATCCCCGGCAAGGTGACGCCCTGTTCCGGGCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTGATCAGTTCCTGCATCTGGATAc                                                                                                                                                <  1:727000/250‑1 (MQ=255)
                                                                                                  ctgaccTGT‑TTCAGGGTTTGCGTTGCCCGTACGTGTTCAGCTTCGGAAGTGCCGATACCAAAGGCCAGTGCGCCACACGCGCCGTG‑GGTGGCGGTATGCGAGTCGCCGCAGACA‑ATGGTCATCCCCGGCAAGGTGACGCCCTGTTCCGGGCCCATTACGTTGACGATCCCCTGATACGGGTGATTCAGGTCCTTCCGTTCGCCGCCCAATTCTTTTCAGTTTTTGATCAGTTCCTGCATCTGTATAcgccc                                                                                                                                            >  2:470594/5‑249 (MQ=255)
                                                                                                   ggCCCTGT‑TGCAGGCTTTGCGTTGCCAGTACGTGTTCAACTTCGGAAGTGCCGATACCAAACGCCAGTGCGCCAAACGCTCCGTG‑GGTGGCCGTATGCGAGTCCCCGCAGCCA‑ATGGTCATCCCCGACACGGTGACCCGCTGTTCCGGGCCCATTACCTGGACGAACCCCTGCTAAGCACAATTCATGTCATACAGCTCGCAGTCAAATACTTTGCAGTTTTTGATCACTTTCTTCACCTGGATCCgcgc                                                                                                                                            >  2:328214/1‑250 (MQ=255)
                                                                                                    gCCCTGT‑TTCAGGGTTTGCGTTGCCAGTACGTGTTCAACTTCGAAAGTGCCGATACCAAAGGCCAGTGCGCCAAACGCGCCGTG‑GGTGGCGGTATGCGAGTCGCCGCAGACA‑ATGGTCATCCCCGGCAAGGGGACGCCCTGTTCCGGGCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTGATCAGTTACTGACTCTGGATAcgagca                                                                                                                                           >  2:231823/1‑246 (MQ=255)
                                                                                                     cccTGT‑TTCAGGGTTTGCGTTGCCAGTACGTGTTCAACTTCGGAAGTGCCGATACCAAAGGCCGGTGCGCCAAACGCGCCGTG‑GGTGGCGGTATGCGAGTCGCCGCAGACA‑ATGGTCATCCCCGGCAAGGTGACGCCCTGTTCCGGGCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTGATCAGTTCCTGCATCTGGATACGCGCCAt                                                                                                                                         >  1:329813/1‑251 (MQ=255)
                                                                                                         gT‑TTCAGGGTTTGCGTTGCCAGTACGTGTTCAACGTGGGATGGGCCGATACCAAAGGCCAGTGCGTCAAACGCGCCGTG‑GGTGGCGTTATGCGAGTCTCCGCAGACA‑ATGGTCATCCCCGGCAAGGTGACGCCCGGTTCCGTGCCGATTACGTTGACGATCCACTGATACGGGTGATTCAGGTCATTCAGTTCGACGCCAAATTCTTTGCAGTTGTTGATCAGTTCCTGCAGCTGGTTACGCGCCAttt                                                                                                                                       <  2:650117/249‑1 (MQ=255)
                                                                                                         gT‑TTAAGGGGTTGCGTTGCCAGTACGTGTTCAACTTCGGAAGTGCCGATACCAAAGGCCAGTGCGCCAAACGCGCCGTG‑GGTGGCGGTATGCGAGTCGCCGCAGACA‑ATGGTCATCCCCGGCAAGGTGACGCCCTGTTCCGGGCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTc                                                                                                                                      <  2:672687/250‑1 (MQ=255)
                                                                                                                  gTTTGCGTTGCCAGTACGTGTTCAACTTCGGAAGTGCCGATACCAAAGGCCAGTGCGCCAAACGCGCCGTG‑GGTGGCGGTATGCGAGTCGCCGCAGACA‑ATGGTCATCCCCGGCAAGGTGACGCCCTGTTCCGGGCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGc                                                                                                                             >  1:523202/1‑251 (MQ=255)
                                                                                                                  gTTTGCGTTGCCAGTACGTGTTCAACTTCGGAAGTGCCGATACCAAAGGCCAGTGCGCCAAACGCGCC‑TGTGGTGGCGGTATGCGAGTCGCCGCAGACA‑ATGGTCATCCCCGGCAAGGTGACGCCCTGTTCCGGGCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGc                                                                                                                             <  2:557880/251‑1 (MQ=255)
                                                                                                                        gTTGCCAGTACGTGTTCAACTTCGGAAGTGCCGATACCAAAGGCCAGTGCGCCAAACGCGCCGTG‑GGTGGCGGAATGCGAGTCGCCGCAGACA‑ATGGTCATCCCCGGCAAGGTGACGCCCTGTTCCGGGCCCATTACGAGGACGCTCCCCTGATACGGGTGATTCGGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAAt                                                                                                                       >  2:935874/1‑251 (MQ=255)
                                                                                                                         ttGCCAGTACGTGTTCAACTTCGGAAGTGCCGATACCAAAGGCCAGAGCGCCAAACGAGCCGTG‑GGTGGCGGTATGCGAGTCGCCGCAGACA‑ATGGTCATCCCCGGCAAGGTGACGCCCTGTTCCGGGCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGACATTactg                                                                                                                      >  1:789631/1‑248 (MQ=255)
                                                                                                                           gCCAGTACGTGTTCAACTTCGGAAGGGCCGCTACCAAAGGCCAGTGCGCCAACCGCGCCGTG‑GGGGGCGGTAGGCGAGTCGCCGCAGACA‑ATGGTCATCCCCGGCAAGGTGACGCCCTGTTCCGGGCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGt                                                                                                                     <  2:342281/250‑1 (MQ=255)
                                                                                                                               gTACGGGTGCAACGTCGGAAGTGCCGATACCAAAGGCCAGTGCGCCAAACGCGCCGTG‑GGTGGCGGTATGCGAGTCGCCGCATACA‑ATGGTCAGCCCCGGCAAGGTGACGCCCTGTTCCGGGCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTGATCAGTTCCTGCATCGGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTg                                                                                                                <  2:920110/251‑1 (MQ=255)
                                                                                                                                 ccGTGTTCAACTTCGGAAGTGCCGATACCAAAGGCCAGTGCGCCAAACGCGCCGTG‑GGTGGCGGTATGCGAGTCGCCGCAGACA‑ATGGTCATCCCCGGCAAGGTGACGCCCTGTTCCGGGCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGt                                                                                                              <  1:818037/250‑1 (MQ=255)
                                                                                                                                  cGTGTTCAACTTCGGAAGTGCCGATACCAAAGGCCAGTGCGCCAAACGCGCCGTG‑GGTGGCGGTATGCGAGTCGCCGCAGACA‑ATGGTCATCCCCGGCAAGGTGACGCCCTGTTCCGGGCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGtt                                                                                                             >  2:8493/1‑250 (MQ=255)
                                                                                                                                  cGTGTTCAACTTCGGAAGTGCCGATACCAAAGGCCAGTGCGCCAAACGCGCCGTG‑GGTGGCGGTATGCGAGTCGCCGCAGACA‑ATGGTCATCCCCGGCAAGGTGACGCCCTGTTCCGGGCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTc                                                                                                             >  1:201321/1‑251 (MQ=255)
                                                                                                                                        cAACTTCGGAAGTGCCGATACCAAAGGCCAGTGCGCCAAACGCGCCGTG‑GGTGGCGGTATGCGAGTCGCCGCAGACA‑ATGGTCATCCCCGGCAAGGTGACGCCCTGTTCCGGGCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTGATCAGTTCCTGCATCTGGATCCGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGtg                                                                                                       >  2:130480/1‑250 (MQ=255)
                                                                                                                                               ggAAGTGCCGATACCAAAGGCCAGTGCGCCAAACGCGCCGTG‑GGTGGCGGTAGGCGAGTCGCCGCAGACA‑ATGGTCATCCCCGGCAAGGTGACGCCCTGTTCCGGGCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGACGt                                                                                                <  2:525389/251‑1 (MQ=255)
                                                                                                                                                    tGCCGATCCCAAAGGCCAGTGCGCCAAACGCGCCGTG‑GGTGGCGGTATGCGAGTCGCCGCAGACA‑ATGGTCATCCCCGGCAAGGTGACGCCCTGTTCCGGGCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTaa                                                                                                                        <  2:689513/222‑1 (MQ=255)
                                                                                                                                                    tGCCGATACCAAAGGCCAGTGCGCCAAACGCGCCGTG‑GGTGGCGGTATGCGAGTCGCCGCAGACA‑ATGGTCATCCCCGGCAAGGTGACGCCCTGTTCCGGGCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTaa                                                                                                                        >  1:689513/1‑222 (MQ=255)
                                                                                                                                                     gCCGATACCAAAGGCCAGTGCGCCAAACGCGCCGTG‑GGTGGCGGTATGCGAGTCGCCGCAGACA‑ATGGTCATCCCCGGCAAGGTGACGCCCTGTTCCGGGCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGACGTTGTGAt                                                                                          >  1:161688/1‑251 (MQ=255)
                                                                                                                                                      cggatcccAAAGGCCGGTGAACAAAACGCGC‑GGG‑GGTGGCGGTATGCGAGTCGCCGCAGACA‑ATGGTCATCCCCGGCAAGGTGACGCCCTGTTCCGGGCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTGGGTCTGGGTAGAGACGTTGGGATcc                                                                                        <  1:187763/245‑1 (MQ=255)
                                                                                                                                                         aTACCAAAGGCCAGTGCGCCAAACGCGCCGTG‑GGTGGCGGTATGCGAGTCGCCGCAGACA‑ATGGTCATCCCCGGCAAGGTGACGCCCTGTTCCGGGCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGACGTTGTGATCCAt                                                                                      <  1:228327/251‑1 (MQ=255)
                                                                                                                                                         aTACCAAAGGCCAGTGCGCCAAACGCGCCGTG‑GGAGGCGGAATACGAGTCGCCGCAGACA‑ATGGACATCCCCTGCCAGGAGACGCCCTGTTCCGGGCCCATTACGAGGACGGTCCCCTGATAAGGGTAATTCAGGACATACAGTTCGCCGCCCACTTCTTTGCAGTTTTTGATCAGTTCCTGCATCTGCATACGCGCCATTTCACCGCCGGCATTAATGTCTTTGGTCTGGGATGAGACGTTGTGTTcctt                                                                                      >  2:172689/1‑249 (MQ=255)
                                                                                                                                                         aTACCAAAGGCCAGGGCGCCAAACGCGCCGTG‑GGTGGCGGTAGGCGAGTCGCCGCAGACA‑ATGGTCCTCCCCGGCAAGGTGACGCCCTGTTCCGGGCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTGATCAGTTCCTTCATCTGGATACTCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGACGTTGTGATCCAt                                                                                      <  2:558605/251‑1 (MQ=255)
                                                                                                                                                          tACCAAAGGCCAGTGCGCCAAACGCGCCGTG‑GGTGGCGGTATGCGAGTCGCCGCAGACA‑ATGGTCATCCCCGGCAAGGTGACGCCCTGTTCCGGGCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGACGTTGTGATCCATg                                                                                     >  1:971014/1‑251 (MQ=255)
                                                                                                                                                           tccAAAGGCCAGGGCGCCAAACGCGCCGTG‑GGTGGCGGTATTCGGGTCGCCGCAGACA‑ATGGGCAACCCCGGCAAGGGGACGCCGTGGTCCGGGCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTCGAGACGTTGTGATCCATg                                                                                     <  2:720781/249‑1 (MQ=255)
                                                                                                                                                                aGGCCAGTGCGCCAAACGCGCCGTG‑GGTGGCGGTATGCGAGTCGCCGCAGACA‑ATGGTCATCCCCGGCAAGGTGCCGCCCTGTTCCGGGCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATTTCTTTGGTCTGGGTGGAGACGTTGAGATCCCTGGTAGCg                                                                               >  2:207465/1‑251 (MQ=255)
                                                                                                                                                                aGGCCAGTGCGCCAAACGCGCCGTG‑GGTGGCGGTATGCGAGTCGCCGCAGACA‑ATGGTCATCCCCGGCAAGGTGACGCCCTGTTCCGGGCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGACGTTGTGATCCATGGTAGCg                                                                               <  1:516947/251‑1 (MQ=255)
                                                                                                                                                                        gcgcCAAACGCGCCGTG‑GGTGGCGGTATGCGAGTCGCCGCAGACA‑ATGGTCATCCCCGGCAAGGTGACGCCCTGTTCCGGGCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGACGTTGTGATCCATGGTAGCGAAGGtttt                                                                       <  1:987538/251‑1 (MQ=255)
                                                                                                                                                                          gcCAAACGCGCCTTG‑GGTGGCCGTATGATCGTCGCCTCAG‑CATATGGTCATCCCCAGCAAGGTGACGCCCTGTGCCGGGCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTTAGACAGTGCGACGCCAAATTCTTTGCAGTTTTTGATCAGTGCCTGCAGCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGACGGTGTGATCCATGGTAGCGAAGGTTTTGc                                                                     <  2:71148/251‑1 (MQ=255)
                                                                                                                                                                              aaCGCGCCGTG‑GGTGGCGGTATGCGAGTCGCCGCAGACA‑ATGGTCATCCCCGGCAAGGTGACGCCCTGTTCCGGGCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCCCCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGAAGTTGTGATCCATGGTAGCGAAGGTTTTGCCCgg                                                                 >  2:511209/1‑251 (MQ=255)
                                                                                                                                                                                cgcgCCGTG‑GGTGGCGGTATGCGAGTCGCCGCAGACA‑ATGGTCATCCCCGGCAAGGTGACGCCCTGTTCCGGGCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGACGTTGTGATCCATGGTAGCGAAGGTTTTGCCCGGCt                                                               >  1:101110/1‑251 (MQ=255)
                                                                                                                                                                                    ccGTG‑GGAGGCGGTATGCGAGTCGCCGCAGACA‑ATGGTCATCCCCGGCAAGGTGACGCCCTGTTCCGGGCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGACTTTGGTCTGGGTAGAGACGTTGTGATCCATGGTAGCGAAGGTTTTGCCCGGCTGACg                                                           >  1:611048/1‑251 (MQ=255)
                                                                                                                                                                                      gtg‑ggtggCGGTATGCGAGTCGCCGCCGACA‑ATGGTCATCCCCGGCAAGGTGACGCCCTGTTCCGGGCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGACGTTGTGATCCATGCACGCGAAGGTTTTGCCCGGCTGACGt                                                          >  2:680576/1‑250 (MQ=255)
                                                                                                                                                                                                     gCGAGTCGCCGCAGACA‑ATGGTCATCCCCGGCAAGGTGACGCCCTGTTCCGGGCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGACGTTGTGATCCATGGTAGCGAAGGTTTTGCCCGGCTGACGTACCGGGCGACCGTgg                                           <  2:201321/251‑1 (MQ=255)
                                                                                                                                                                                                              cgcAGACA‑ATGGTCATCCCCGGCAAGGTGACGCCCTGTTCCGGGCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGACGTTGTGATCCATGGTAGCGAAGGTTTTGCCCGGCTGACGTACCGGGCGAcc                                               >  1:374689/1‑238 (MQ=255)
                                                                                                                                                                                                              cgcAGACA‑ATGGTCATCCCCGGCAAGGGGACGCCCTGTTCCGGGCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGACGTTGTGATCCATGGTAGCGAAGGTTTTGCCCGGCTGACGTACCGGGCGAcc                                               <  2:374689/238‑1 (MQ=255)
                                                                                                                                                                                                                       aTGGTCATCCCCGGCAAGGTGACGCCCTGTTCCGGGCCCATTACGTGGACGATCCCCTGATACGGGTGTTTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGACGTTGTGATCCATGGTAGCGAAGGTTTTGCCCGGCTGACGTACCGGGCGACCGTGGGCGCGCAGACCATCGa                           >  1:66538/1‑250 (MQ=255)
                                                                                                                                                                                                                            cATCCCCGGCAAGGTGACGCCCTGTTCCGGGCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGACGTTGTGATCCATGGTAGCGAAGGTTTTGCCCGGCTGACGTACCGGGCGACCGTGGGCGCGCAGACCATCGAACGCCt                     >  1:313339/1‑251 (MQ=255)
                                                                                                                                                                                                                               ccccGGCAAGGTGACGCCCTGTTCCGGGCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGACGTTGTGATCCATGGTAGCGAAGGTTTTGCCCGGCTGACGTACCGGGCGACCGTGGGCGCGCAGACCATCGAACGCCTGCg                  >  1:833918/1‑251 (MQ=255)
                                                                                                                                                                                                                                 ccgtcAAGGTGACGCCCGGTTCCGGGCCCATTACTTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGCCGCCAAATTCTTTGCAGTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGGCTGGGTAGAGACGTTGTGATCCATGGTAGCGAAGGTTTTGCCCGGCTGACGTACCGGGCGACCTTGGGCGCGCAGACCAGCGAACGCCTGCGGt                <  2:597059/247‑1 (MQ=255)
                                                                                                                                                                                                                                   gtcaagggtaCGCCCTGTGCCGGCCCCACTACGTGTACGAGCCCCTGTTACGGGTGATTCTGGTCATACATTTCGACGCCTAATTCTTTGCAGTTTGTGAGCAGTTCCTGCATCTGGATTCGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGACGTTGTGATCCATGGTAGCGAAGGTTTTGCCCGGCTGACGTAGCGGGCGACCGTGGGCGCGCAGACCATCGAACGCCTGCGGTGa              <  2:176126/242‑1 (MQ=255)
                                                                                                                                                                                                                                   ggCAAGGTGACGCCCTGTTCCGGGCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGACGTTGTGATCCATGGTAGCGAAGGTTTTGCCCGGCTGACGTACCGGGCGACCGTGGGCGCGCAGACCATCGAACGCCTGCGGTg               >  2:925695/1‑250 (MQ=255)
                                                                                                                                                                                                                                      aaGGTGACGCCCTGTTCCGGGCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGACGTTGTGATCCATGTTAGCGAAGGTTTTGCCCGGCTGACGTACCGTGCGACCGTGGGCGAGCAGACCATCGACCGCCTGCGGTGAggg           >  2:111378/1‑250 (MQ=255)
                                                                                                                                                                                                                                         gTGACGCCCTGTTCCGGGCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGACGTTGTGATCCATGGTAGCGAAGGTTTTGCCCGGCTGACGTACCGGGCGACCGTGGGCGCGCAGACCATCGAACGCCTGCGGTGAGGTCAc        <  1:701503/251‑1 (MQ=255)
                                                                                                                                                                                                                                          tGACGCCCTGTTCCGGGCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGACGTTGTGATCCATGGTAGCGAAGGTTTTGCCCGGCTGACGTACCGGGCGACCGTGGGCGCGCAGACCATCGAACGCCGGCGGTGAGGTCACt       >  1:982324/1‑251 (MQ=255)
                                                                                                                                                                                                                                             cGCCCTTTTCCTGGCCCATTTCGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGACGTTGTGATCCATGGTAGCGAAGGTTTTGCCCGGCTGACGTACCGGGCGACCGTGGGCGCGCAGACCATCGAACGCCTGCGGTGAGGTCACTTCa    <  2:638802/251‑1 (MQ=255)
                                                                                                                                                                                                                                              gCCCTGTTCCGGGCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGACGTTGTGATCCATGGTAGCGAAGGTTTTGCCCGGCTGACGTACCGGGCGACCGTGGGCGCGCAGACCATCGAACGCCTGCGGTGAGGTCACTTCAt   <  1:879006/251‑1 (MQ=255)
                                                                                                                                                                                                                                              ccccTTTTCCGGGCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGTTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTGATCAGTTCCTGCATCTGGATACGCGTCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGACGTTGTGATCCATGGTAGCGAAGGTTTTGCCCGGCTGACGTACCGGGCGACCGTGGGCGCGCAGACCATCGAACGCCTGCGGTGAGGTCACTTCAt   <  1:961911/250‑1 (MQ=255)
                                                                                                                                                                                                                                               cccTGTTCCGGGCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGACGTTGTGATCCATGGTAGCGAAGGTTTTGCCCGGCTGACGTACCGGGCGACCGTGGGCGCGCAGACCATCGAACGCCTGCGGTGAGGTCACTTCATg  <  1:71070/251‑1 (MQ=255)
                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                               
CTGCGCTACCGGTTTTACCGATAATTGCCAGCACGATATCTTTTGCGGTAATGCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTCATGGTTTTTGCGCGGCCCTGT‑TTCAGGGTTTGCGTTGCCAGTACGTGTTCAACTTCGGAAGTGCCGATACCAAAGGCCAGTGCGCCAAACGCGCCGTG‑GGTGGCGGTATGCGAGTCGCCGCAGACA‑ATGGTCATCCCCGGCAAGGTGACGCCCTGTTCCGGCCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGACGTTGTGATCCATGGTAGCGAAGGTTTTGCCCGGCTGACGTACCGGGCGACCGTGGGCGCGCAGACCATCGAACGCCTGCGGTGAGGTCACTTCATG  >  NC_000913/80282‑80768

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑