Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	65,202	G→A	F193F (TTC→TTT)	polB ←	DNA polymerase II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	65,202	0	G	A	99.0%	317.1 / NA	98	F193F (TTC→TTT)	polB	DNA polymerase II
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (49/47);  minor base C (1/0);  total (51/47)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.79e-01

GATAATTAGCCGACCTTTAGCCTGGGCAAAAAA‑GACG‑CCGTTTTTAAAGCCGTGCTCGCGCC‑ACTCCAGCTCGCTATTATCGCGCCCAAGACGCAGCGGAAGACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGCAACTGCGGGCGGCTGGCGACGTATTCCAGTTCGAAATCAAGCGAGGAGGCGTCGCCATTCTCCGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAATATCTA‑TAGAAACCCACTTGAGCGGCGGA‑CGATAGT‑CGGGATGCGGTTTCAGACGGGCATTAACGATAGTGCCATTGTGCA‑TATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCTCCATCAGATAGCGT  >  NC_000913/64956‑65445                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                        gATAATTAGCCGACCTTTAGCCTGGGCAAAAAA‑GACG‑CCGTTTTTAAAGCCGTGCTCGCGCC‑ACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAAt                                                                                                                                                                                                                                                     >  1:799297/1‑251 (MQ=255)     aTTAGCCGACCTTTAGCCTGGGCAAAAAA‑GACG‑CCGTTTTTAAAGCCGTGCTCGCGCC‑ACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATcg                                                                                                                                                                                                                                                   >  1:545960/1‑248 (MQ=255)        aGCCGACCTTTAGCCTGGGCAAAAAA‑GCCG‑CCGTTTTTAAAGCCGTGCTCGCGCC‑ACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCgc                                                                                                                                                                                                                                              >  1:330562/1‑250 (MQ=255)          ccGACCTTTGGCCGGGGCAAAAAATGA‑G‑CCGTTTTTAAAGCAGTGCCCGCGCC‑ACGCCAGCTCACTGTTAACGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTGCCAACCGATGATCACAGCAGGATCTTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGGAGCTGCGGGCGTCTGGCGACGTATTCCAGTTTAAAATCGAGCgcgg                                                                                                                                                                                                                                            <  2:97478/251‑4 (MQ=255)           cGACCTTTAGCCTGGGCAAAAAA‑GACG‑CCGTTTTTAAAGCCGTGCTCGCGCC‑ACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCgcgga                                                                                                                                                                                                                                           <  1:228499/251‑1 (MQ=255)                 ttaaccTGGGCAAAAAA‑GACG‑CCGTTTTTAAAGCCGTGCTCGCGCCCAC‑CCAGCTCACGGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCg                                                                                                                                                                                                                                       <  2:462254/245‑1 (MQ=255)                    cccTGGGAAAAAAA‑G‑CGTCCGTTTTTAAAGCCTTGCTCGCGCC‑CCTCCATCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGGTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTGCAAAATCGAGCGCGGATGCGTCGcc                                                                                                                                                                                                                                  <  2:478131/250‑1 (MQ=255)                        gggCAAAAAA‑GACG‑CCGTTTTTAAAGCCGTGCTCGCGCC‑ACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAAACAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGACGCCATtc                                                                                                                                                                                                                              >  1:731196/1‑251 (MQ=255)                          gCAAAAAA‑GACG‑CCGTTTTTAAAGCCGTGCTCGCGCC‑ACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATtctc                                                                                                                                                                                                                            >  2:515522/1‑251 (MQ=255)                             aaaaa‑GACG‑CCGTTTTTAAAGCCGTGCTCGCGCC‑ACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATtctctgg                                                                                                                                                                                                                         >  1:599190/1‑248 (MQ=255)                                aa‑GACG‑CCGTTTTTAAAGCCGTGCTCGCGCC‑ACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGccc                                                                                                                                                                                                                      >  2:227137/1‑251 (MQ=255)                                        ccGTTTTTAAAGCCGTGCTCGCGCC‑ACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCCTGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGCGGCGGCCCCATTATCTGGCCCCAGcct                                                                                                                                                                                                                >  2:672799/1‑249 (MQ=255)                                           tttttAAAGCCGTGCTCGCGCC‑ACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCatat                                                                                                                                                                                                              >  1:516656/1‑250 (MQ=255)                                           tttttAAAGCCGTGCTCGCGCC‑ACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGCAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCatat                                                                                                                                                                                                              >  1:447486/1‑250 (MQ=255)                                             tttAAAGCCGTGCTCGCGCC‑ACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATaa                                                                                                                                                                                                            >  1:288511/1‑250 (MQ=255)                                                aaaGCCGTGCTCGCGCC‑ACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGAATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCCCTGGCCCCAGCCTACAAACg                                                                                                                                                                                                         >  2:948984/1‑250 (MQ=255)                                                    ccGTGCTCGCGCC‑ACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGACGCCCTTCTCTGGCCCCAGCATAAAAACGATggg                                                                                                                                                                                                    >  2:542203/1‑249 (MQ=255)                                                       tGCTCGCGCC‑ACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTg                                                                                                                                                                                                 >  1:213774/1‑251 (MQ=255)                                                        gcactcgcC‑CCTCCATCTCACTGTTATCTCGCCCCAGCCGCAGCTGAATACTGTAACGCTCGGCATGTTTTTGCAGCATTCGCATATCGAACTGCACCCCGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAGCGATGCGCTg                                                                                                                                                                                                 <  1:212058/245‑1 (MQ=255)                                                          ccgcgCC‑CCTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGccc                                                                                                                                                                                              <  1:104840/250‑1 (MQ=255)                                                           cgcgCC‑ACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCg                                                                                                                                                                                             >  1:868290/1‑251 (MQ=255)                                                           cgcgCC‑ACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCg                                                                                                                                                                                             <  1:746808/251‑1 (MQ=255)                                                              gcC‑ACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAg                                                                                                                                                                                          <  1:892895/251‑1 (MQ=255)                                                                c‑ACTCCAGCTCACTGTTATAGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCAGATGGCGACGTAATCCAGTTCAAAAACGAGCGCGGAGGCGTCGCCATTCGCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGcc                                                                                                                                                                                        >  2:570391/1‑251 (MQ=255)                                                                   cTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATACACGATGCGCAGCCCGCATCCtt                                                                                                                                                                                      >  2:820948/1‑251 (MQ=255)                                                                     ccagcgcactgttatCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCtt                                                                                                                                                                                      <  1:297300/238‑1 (MQ=255)                                                                     ccAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCTGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCtt                                                                                                                                                                                      <  1:429466/249‑1 (MQ=255)                                                                     ccAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGGAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCtt                                                                                                                                                                                      <  1:86245/249‑1 (MQ=255)                                                                      cagccccctgttatCGCCCCCCAGCCGCATCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGACGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCa                                                                                                                                                                                   <  2:288511/241‑1 (MQ=255)                                                                      cAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCa                                                                                                                                                                                   <  1:908327/251‑1 (MQ=255)                                                                          tcactgttatCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGcc                                                                                                                                                                               >  2:235742/7‑251 (MQ=255)                                                                             ctgttatCGCGCCCCAGACGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCACCCGATGATCACATCGGGATCGTAGTTGGCAAACCCGGCGTTGAGTTTTTCCAATAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCACAATCGCGCGCGGAGGCCTCGCCCTTCTCTGGCACCAGAATTTACACGATCCGCTACCCGACGCCTTCAAGGCCGAt                                                                                                                                                                            >  2:581515/4‑251 (MQ=255)                                                                               gttatCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGAt                                                                                                                                                                            <  1:235742/248‑1 (MQ=255)                                                                                    cgcgCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGAGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTAc                                                                                                                                                                     >  1:728617/1‑251 (MQ=255)                                                                                       gccccagccGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGAAGCCTTCCAGGCCGATGCAGTACAGc                                                                                                                                                                  >  2:787542/9‑251 (MQ=255)                                                                                          ccagccGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTc                                                                                                                                                                >  1:527515/6‑250 (MQ=255)                                                                                           cagccGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACCGCTCAc                                                                                                                                                              >  1:325352/5‑251 (MQ=255)                                                                                                  aGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCg                                                                                                                                                       >  1:118419/1‑251 (MQ=255)                                                                                                      gaatccgtgtaCGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGGTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATTCAGTACAGCTCACCGTGGCGggtg                                                                                                                                                   <  2:213774/241‑1 (MQ=255)                                                                                                      gaataCGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGATGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGggtg                                                                                                                                                   <  1:807709/247‑1 (MQ=255)                                                                                                           cGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTACAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGCCCGATGCAGTACAGCTCACCGTGGCGGGGGGtttt                                                                                                                                              >  2:67137/1‑250 (MQ=255)                                                                                                                aCGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTTGTTTCAatat                                                                                                                                         >  1:283723/1‑251 (MQ=255)                                                                                                                   cTCGGCATGTTTTTGCAGCCTTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGGGTTTTTCCAGTAGCTGCGGGCGGCTGGCGCCGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGCTGCAGTACCGCTCACCAGGGCGGGGGGTGTCAatataa                                                                                                                                       >  2:629644/1‑248 (MQ=255)                                                                                                                     cGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCCGTACAGCTCACCGTGGCGGGAGGGTTCAATATcaaat                                                                                                                                    >  2:81235/1‑247 (MQ=255)                                                                                                                        cctGTTTTTGCAGCATTCGCAGATCGAACTGCACCACTTTCCAACTGATGATCTAATCAGGCTCGTAGTTGGCCGACCAGGCGTTGAGTTTTTCCAGTCGCTGCGGTCGGCTGGCGACGTATTCCAGTTCTAAATCGAGAGCGGAGGCGTCGCCATTCTCTGGCCCACCCATATAACCGATGCTCTGCCCGCCGCCTTACAGTCCCATGCAGTACAGCTCACCTTGACGgttggtctctatctacaaggg                                                                                                                                  >  2:914169/3‑235 (MQ=255)                                                                                                                        cATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTCCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTTGTTTCAATATCAA‑TAGa                                                                                                                                 >  1:341309/1‑250 (MQ=255)                                                                                                                           gTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGGTTCAATATcaaaaaaaacc                                                                                                                              >  1:674666/1‑241 (MQ=255)                                                                                                                               tggtagcaTTCGCAGATCGAATTGCCCCACGTGCCAACCGATTGTCACCTCAGGCTCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGGAGCTGCGTGCGGCTGGCGATGGTTTCCAGTCCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGTTGCCCGCAGCCTTCCAGGCCGATGCAGGACAGCTCACCGTGGCTGGTGGTTTCAATATCAA‑TAGACACCCAc                                                                                                                          <  2:824414/246‑1 (MQ=255)                                                                                                                                tGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAATATCAA‑TAGAAACCCACt                                                                                                                         <  1:651521/250‑1 (MQ=255)                                                                                                                                tGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGGGGTTTCAATATCTA‑AAGAAACCCACtt                                                                                                                        >  1:290196/1‑251 (MQ=255)                                                                                                                                  cagcaTTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGGGGTTTCAATATCAA‑TAAAAACCCAcatg                                                                                                                       >  2:610936/1‑247 (MQ=255)                                                                                                                                   agcaTTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGGGGTTTCAATATCAA‑TAGAAACCCACTTg                                                                                                                       >  1:806150/1‑249 (MQ=255)                                                                                                                                       ttCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGGGGGTTCAATATCAA‑TAGAAAACCACTTGAgcggg                                                                                                                 >  1:358069/1‑250 (MQ=255)                                                                                                                                           gaGATCGAACTGCACCCGGTTGCATCCGATGATCACATCAGGATCGTAGTTGGCAAACCAGTCTTTGAGTTTTTCCAGGAGCTGCGGGCGTCTGGCGACTTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCACACCGTGGCGGGTGGTTTCAATATCAA‑TAGAAACCCACTTTAGCGGCGGA‑c                                                                                                            <  2:72330/250‑1 (MQ=255)                                                                                                                                            aGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAATATCAA‑TAGAAACCCACTTGAGCGGCGGA‑Cg                                                                                                           <  1:809482/251‑1 (MQ=255)                                                                                                                                             gATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAATATCAA‑TAGAAAACCACTTAAGcggcgggcgg                                                                                                           >  1:79113/1‑247 (MQ=255)                                                                                                                                                cGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGCGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGGGGCGGGGGGGTTCTATATCAAATAAAAAACCA‑TTGAGcggcgggggaa                                                                                                          >  2:523670/1‑244 (MQ=255)                                                                                                                                                      gCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAATATCAA‑TAGAAACCCACTTGAGCGGCGGA‑AGATAGT‑Cgggg                                                                                                >  1:268797/1‑250 (MQ=255)                                                                                                                                                       caccacGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGGTTCAATATCAA‑AAGAAACCCACTTGAGCGGCGGA‑CGGTAGT‑Cggg                                                                                                 >  1:582609/1‑249 (MQ=255)                                                                                                                                                       caccacGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGGGGGTTCAATATCCA‑TAGAAACCCCCTTGAGCGGCGGG‑CGATAGT‑Cggggg                                                                                               >  1:838653/1‑249 (MQ=255)                                                                                                                                                        aacacGTTCCAACCGATGATCACATCAGGATCGTGGTTGGCAATACAGGCGGTGAGGTTTCCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCGCACCGTGGCGGGTGGTTTCAATATCAA‑TAGAAACCCACTTGAGCGGCGGA‑CGATAGT‑CGGGATg                                                                                              <  2:316078/249‑1 (MQ=255)                                                                                                                                                         ccacGTTCCAACCGAGGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCTATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAATATCAA‑TAGAAACCCACTTGAGCGTCGGA‑CGATAGT‑CGGGATGc                                                                                             <  1:581017/251‑1 (MQ=255)                                                                                                                                                          cacgtgccaccctagggtcccgacAGGATCGTAGTTGGCAAACCTGGGGTTGAGTCTTTCCAGGAGCTGCGGGCGGCTGGCGACGTATTCCAGTGCAAAAGCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCTGCCTTCCAGGCCTATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAATATCTA‑TAGAAACCCACTTGAGCGGCGGA‑CGATAGT‑CGGGATGct                                                                                            <  2:994266/228‑2 (MQ=255)                                                                                                                                                              ttCCAACCGATGATCACCTCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCCAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGGGGGTTAAATCTCGG‑TAGAAAACCACGTTAGAGGCGGG‑CGGTAGT‑Cggggtgggggtt                                                                                        >  1:108069/1‑242 (MQ=255)                                                                                                                                                               tCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGGGGCGGGTGGGTTCAATATCCA‑TAGAAACACAATTGTGAGGGGGG‑CGATAGT‑CGGGGTGGGGtttt                                                                                       >  2:713780/1‑250 (MQ=255)                                                                                                                                                                ccAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAATATCAA‑TAGAAACCCACTTGAGCGGCGGA‑CGATAGT‑CGGGATGCGGTTTCa                                                                                      <  1:787542/251‑1 (MQ=255)                                                                                                                                                                 cAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCTGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGTCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAATATCAA‑TAGAAACACACTTGAGCGGCGGA‑CGATAGT‑CGGGATGCGGTTTCAg                                                                                     <  1:848132/251‑1 (MQ=255)                                                                                                                                                                      gatgataccaTCAGGACCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGGCGCCATTCTCTGGCCCCAGCATAGAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTGCAATATCAA‑TAGAAACCCACTTGAGCGGCGGA‑CGATAGT‑CGGGATGCGGTTTCAGACggg                                                                                <  2:868290/243‑1 (MQ=255)                                                                                                                                                                        tgatCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCCGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGCTGCGCTGCCCGCAGCCTTCCCGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCCATCTCAA‑TAAAAACCCCCTTGCGCGGAGGA‑CGGTTAT‑CGGGATGCGGTTTCCGACGGGc                                                                               >  1:179979/1‑250 (MQ=255)                                                                                                                                                                         gatCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGGTTCAATATAAA‑TAGAAACCCACTTGAGCGGCGGG‑CGATAGT‑CGGGGTGCGGGTTCAGGAGGGCAt                                                                             >  1:800737/1‑251 (MQ=255)                                                                                                                                                                             acaTCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAATCTCAA‑TAGAAACCCCCTTTGGCGGCGGG‑CGATATG‑CGGGATGCGGTTTCAGACGGGCaattac                                                                         >  1:691599/1‑246 (MQ=255)                                                                                                                                                                              caTCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAATATCAA‑TAGAAACCCACTTGAGCGGCGGA‑CGATAGT‑CGGGATGCGGTTTCAGACGGGCATTAACg                                                                        <  1:943130/251‑1 (MQ=255)                                                                                                                                                                                           gTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGACTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAATATCAA‑TAGAAACCCCCTTGAGCGGCGGG‑CGATAGA‑CGGGGTGCGGGTTCAGACGGGCATTAACGATAGCGCCATtgt                                                           >  1:265431/1‑251 (MQ=255)                                                                                                                                                                                             tGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAATATCAA‑TAGAAACCCACTTGAGCGGCGGA‑CGATAGT‑CGGGATGCGGTTTCAGACGGGCATTAACGATAGCGCCATtgtg                                                          <  1:515522/250‑1 (MQ=255)                                                                                                                                                                                              ggCAAACCAGGCGTTGAGTTTTGCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAATATCAA‑TAGAAACCCACTTGAGCGGCGGA‑CGATAGT‑CGGGATGCGGTTTCAGACGGGCATTAACGATAGCGCCATTGTGCa                                                        <  1:581515/251‑1 (MQ=255)                                                                                                                                                                                                  aaCCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAATATCAA‑TAGAAACCCACTTGAGCGGCGGA‑CGATAGT‑CGGGATGCGGTTTCAGACGGGCATTAACGATAGCGCCATTGTGCA‑TATc                                                   <  1:153279/251‑1 (MQ=255)                                                                                                                                                                                                  aaCCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAATATCAA‑TAGAAACCCACTTGAGCGGCGGA‑CGATAGT‑CGGGATGCGGTTTCAGACGGGCATTAACGATAGCGCCATTGTGCA‑TATc                                                   <  1:48046/251‑1 (MQ=255)                                                                                                                                                                                                   accaggatttGATTTTTTCCAGTAGCTGCGGGTGGCTGGCGACGTATTCCCGTTCAAAATCGAGCGCGTCGGCCTCGCCATTCTCTGGTCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCTCCGTGGCGGGTGGTTTCAATATCAA‑TAGAAACCCCCTTGAGCGGCGGA‑CGATAGT‑CGGGTTGCGGTTTCAGACGGGCATTAACGATAGCGCCATTGTGCA‑TATCa                                                  <  1:720123/243‑1 (MQ=255)                                                                                                                                                                                                     cAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAATATCAA‑TAGaaa                                                                                                                               <  1:522395/175‑1 (MQ=255)                                                                                                                                                                                                          gTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAATATCAA‑TAGAAACCCACTTGAGCGGCGGA‑CGATAGT‑CGGGATGCGGTTTCAGACGGGCATTAACGATAGCGCCATTGTGCA‑TATCACCCTCGa                                           <  1:227137/251‑1 (MQ=255)                                                                                                                                                                                                            tGAGTTTTTCCAGTAGTGGCCGGCGGTTGGCGACGTATGCCAGTTAAAAATCGAGCGCGGAGGCGTCGCAATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTGCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAATAGCAA‑TAGAAACCCACTTGAGCGGCTGA‑CGATAGT‑CGGGATGCGGTTTCAGACGGTCATTAACGATAGCGCCATTGTGCA‑TATCACCCTCGAc                                          <  2:327960/250‑1 (MQ=255)                                                                                                                                                                                                             gaatttttCCAGGAGCGGCGGGCGGGTGGCGACGTATTCCAGTGCAAAACGTAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAATATCAA‑TAGAAACCCACTTGAGCGGCGGA‑CGATAGT‑CGGGATGCGGTTTCAGACGGGCATTAACGATAGCGCTATTGTGCA‑TATCACCCTCGAccc                                        <  2:120250/248‑1 (MQ=255)                                                                                                                                                                                                             gAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAATATCAA‑TAAAAACCCCATTGAGCGGCGGG‑CGATAGT‑CGGGGTGCGGTTTCAGACGGGCATTAACGATAGCGCCATTGTGCA‑TATCACCCTCGAccc                                        >  1:983954/1‑251 (MQ=255)                                                                                                                                                                                                              aGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCTTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAATATCAA‑TAGAAACCCACTTGAGCGGCGGA‑CGATAGT‑CGGGATGCGGTTTCAGACGGGCATTAACGATAGCGCCATTGTGCA‑TATCACCCTCGACCca                                       <  1:747526/251‑1 (MQ=255)                                                                                                                                                                                                                    tccagtagcTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAATATCAA‑TAGAAACCCACTTGAGCGGCGGA‑CGATAGT‑CGGGATGCGGTTTCAGACGGGCATTAACGATAGCGCCATTGTGCA‑TATCACCCTCGACCCACACCg                                  <  1:324011/242‑1 (MQ=255)                                                                                                                                                                                                                      cagtagcTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAATATCAA‑TAGAAACCCACTTGAGCGGCGGA‑CGATAGT‑CGGGATGCGGTTTCAGACGGGCATTAACGATAGCGCCATTGTGCA‑TATCACCCTCGACCCACACCggtg                               <  1:919965/245‑1 (MQ=255)                                                                                                                                                                                                                          agcTGCGGTCGGCTGGCGACGTATTCCATTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAATATCAA‑TAGAAACCCACTTGAGCGGCGGA‑CGATAGT‑CGGGATGCGGTTTCAGACGGGCATTAACGATAGCGCCATTGTGCA‑TATCACCCTCGACCCACACCggtgagg                            <  2:246798/248‑1 (MQ=255)                                                                                                                                                                                                                           gcTGCGGGCGGCTGGCGACGTATTCAAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGGGGGTTCCATATCAA‑AAGAAACCCCCTTTAGCGGCGGG‑CGATAGTTGGGGAGGGGGTTT‑AGACCGGCGTTAACACTAGCGCCATGTTGCA‑TATCACCCCCCACCCACCCCggggggggg                          >  2:65218/2‑244 (MQ=255)                                                                                                                                                                                                                                gggcggCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAGCGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAATATCAA‑TAGAAACCCACTTGAGCGGCGGA‑CGATAGT‑CGGGATGCGGTTTCAGACGGGCATTAACGATAGCGCCATTGTGCA‑TATCACCCTCGACCCACACCGGTGAGGTGATaaa                     <  1:727347/251‑1 (MQ=255)                                                                                                                                                                                                                                gggcggCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCCATATCAA‑TAGAAACCCACTTGAGCGGCGGA‑CGATAGT‑CGGGATGCGGGTTCAGACGGGCATTAACGCAAGAGCCCTTGTGCG‑TATCACCCCCGACCCACACCggtgaggggctaa                      >  1:91370/1‑244 (MQ=255)                                                                                                                                                                                                                                gggcggCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAATATCAA‑TAGAAACCCACTTGAGCGGCGGA‑CGATAGT‑CGGGATGCGGTTTCAGACGGGCATTAACGATAGCGCCATTGTGCA‑TATCACCCTCGACCCACACCGGTGAGGTGATaaa                     <  1:672799/251‑1 (MQ=255)                                                                                                                                                                                                                                    ggcTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGGGGTTTCAATATCAA‑TAGAAACCCACTTGAGCGGCGGA‑CGATAGT‑CGGGATGCGGTTTCAGACGGGCATTAACGATAGCGCCATTGTGCA‑TATCACCCTCGACCCACCCCGGTGGGGTGATAAACCGc                 >  1:258590/1‑251 (MQ=255)                                                                                                                                                                                                                                     gcTGGCGACGTATTCCAGTTCAAAATCGATCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGATTCAATATCAA‑AAGAAACCCACTTGAGGGGCGGAAGGATA‑T‑CCGGGTGGGGTTTTAGACGGGGATTAACGAAAGCGCCATTTTGAAACACCACC‑TCGATCCCCACCGGGGAGGGGATAAACCGcc                >  1:162664/1‑250 (MQ=255)                                                                                                                                                                                                                                        ggCGACGTATTCCAGTTCAAAAGCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCGCACCGTGGCGGGTGGTTTCAATAGCAA‑TAGAAACCCACTTGAGCGGCGGA‑CGATAGT‑CGGGATGCGGTTTCAGACGGGCATTAACGATAGCGCCATTGTGCA‑TATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCTc               <  2:293319/249‑1 (MQ=255)                                                                                                                                                                                                                                         gCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAATATCAA‑TAGAAACCCACTTGAGCGGCGGA‑CGATAGT‑CGGGATGCGGTTTCAGACGGGCATTAACGATAGCGCCATTGTGCA‑TATCAACCTCGACCCACACCGGTGAGGTGATAAACCGCTCCAt            <  1:229483/251‑1 (MQ=255)                                                                                                                                                                                                                                                aTTCCAGTTCAAAACCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAATATCAA‑TAGAAACCCACTTGAGCGGCGGA‑CGATAGT‑CGGGATGCGGTTTCAGACGGGCATTAACGATAGCGCCATTGTGCA‑TATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCTCCATCAGATAg     <  2:268797/251‑1 (MQ=255)                                                                                                                                                                                                                                                   ccAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAATATCAA‑TAGAAACCCACTTGAGCGGCGGA‑CGATAGT‑CGGGATGCGGTTTCAGACGGGCATTAACGATAGCGCCATTGTGCA‑TATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCTCCATCAGATAGCg   <  1:128705/250‑1 (MQ=255)                                                                                                                                                                                                                                                   ccAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAATATCAA‑TAGAAACCCACTTGAGCGGCGGA‑CGATAGT‑CGGGATGCGGTTTCAGACGGGCATTAACGATAGCGCCATTGTGCA‑TATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCTCCATCAGATAGCGt  <  1:194925/251‑1 (MQ=255)                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                        GATAATTAGCCGACCTTTAGCCTGGGCAAAAAA‑GACG‑CCGTTTTTAAAGCCGTGCTCGCGCC‑ACTCCAGCTCGCTATTATCGCGCCCAAGACGCAGCGGAAGACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGCAACTGCGGGCGGCTGGCGACGTATTCCAGTTCGAAATCAAGCGAGGAGGCGTCGCCATTCTCCGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAATATCTA‑TAGAAACCCACTTGAGCGGCGGA‑CGATAGT‑CGGGATGCGGTTTCAGACGGGCATTAACGATAGTGCCATTGTGCA‑TATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCTCCATCAGATAGCGT  >  NC_000913/64956‑65445

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑