Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 77,027 A→G D91D (GAT→GAC)  sgrR ← DNA‑binding transcriptional dual regulator SgrR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091377,0270AG96.4% 436.3 / 3.3 139D91D (GAT→GAC) sgrRDNA‑binding transcriptional dual regulator SgrR
Reads supporting (aligned to +/- strand):  ref base A (2/3);  new base G (61/73);  total (63/76)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.29e-01

Only 100 of 139 total aligned reads displayed.

CTGCCAGTGGTGGGCGATGTCTGCTTCCAGTTCCCCATTTTCCTCA‑TTTATGCGCGTTAGCGAAC‑TGAAGATTTGCCGGGCGATATGGGTTTCGGAACGGCGCAATGCGCTGCCAGGTAGCAGATTACGCAACGGACGATAGTAGAGCACGCGCAGGATGTGCCGCCCCTGGCGGAAGCTGCGGCCCAGATGAGAAACCAGCATTTGCCGCACAGTCGCTTTGTCGCCAACCAACTGCACCAGT‑TGATCGATACGATCCTGCTCCAGCAGGTCTTCCGCCCGCTGTTGCTGAAGCGCCAGCCCGGTATAGAGGAATGTCAGACGCGAGCGTTTACCGCGCCCGACTTCCGCTTCCCACGTCAGCCAGCCGCGATCCTGCATGGTGTTGAGCAGGGTGCGCATATGACGACGCGAGCAGCTCAATAACGCTGCCAGTTCGTTGAGCGTTGTGTCCTGCGATTTACCCTCGCAGCATTGCCACAGGCGGATGAA  >  NC_000913/76781‑77272
                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                     
ctgcCAGTGGTGGGCGATGTCTGCTTCCAGTTCCCCATTTTCCTCA‑TTTATGCGCGTTAGCGAAC‑TGAAGATTTGCCGGGCAATATGGGTTTCGGAACGGCGCAATGCGCTGCCAGGTAGCAGATTACGCAACGGACGATAGTAGAGCACGCGCAGGATGTGCCGCCCCTGGCGGAAGCTGCGGCTCAGATGAGAAACCAGCATTTGCCGCACAGTCGCTTTGTCGCCAACCAACTGCACTAGT‑TGGTCg                                                                                                                                                                                                                                                    >  1:902322/1‑250 (MQ=255)
 gcccgtgggtggGC‑ATGTCCGCCTCCAATTCCCCCTTTTCCTCAGTTTA‑GCCCGGTAACGAACCTGAGGATT‑GCCGGGCAATATGGGTTTCGGAACGGCGCAATGCGCTGCCAGGTAGCAGATTACGCAACGGACGATAGTAGAGCACGCGCAGGATGTGCCGCCCCTGGCGGAAGCTGCGGCCCAGATGAGAAACCAGCATTTGCCGCACAGTCGCTTTGTCGCCAACCAACTGCACTAGT‑TGGTCGATa                                                                                                                                                                                                                                                 <  1:367358/244‑1 (MQ=255)
  gcCAGTGGTGGGCGATGTCTGCTTCCAGTTCCCCATTTTCCTCA‑TTTATGCGCGTTAGCGAAC‑TGAAGATTTGCCGGGCAATATGGGTTTCGGAACGGCGCAATGCGCTGCCAGGTAGCAGATTACGCAACGGACGATAGTAGAGCACGCGCAGGATGTGCCGCCCCTGGCGGAAGCTGCGGCCCAGATGAGAAACCAGCATTTGCCGCACAGTCGCTTTGTCGCCAACCAACTGCACTAGT‑TGGTCGAt                                                                                                                                                                                                                                                  >  1:437527/1‑250 (MQ=255)
    cAGTGGTGGGCGATGTCTGCTTCCAGTTCCCCATTTTCCTCA‑TTTATGCGCGTTAGCGAAC‑TGAAGATTTGCCGGGCAATATGGGTTTCGGAACGGCGCAATGCGCTGCCAGGTAGCAGATTACGCAACGGACGATAGTAGAGCACGCGCAGGATGTGCCGCCCCTGGCGGAAGCTGCGGCCCAGATGAGAAACCAGCATTTGCCGCACAGTCGCTTTGTCGCCAACCAACTGCACTAGT‑TGGTCGATACg                                                                                                                                                                                                                                               >  1:421285/1‑251 (MQ=255)
      gtggtgGGCGATGTCTGCTTCCAGTTCCCCATTTTCCTCA‑TTTATGCGCGTTAGCGAAC‑TGAAGATTTGCCGGGCAATATGGGTTTCGGAACGGCGCAATGCGCTGCCAGGTAGCAGATTACGCAACGGACGATAGTAGAGCACGCGCAGGATGTGCCGCCCCTGGCGGAAGCTGCGGCCCAGATGAGAAACCAGCATTTGCCGCACAGTCGCTTTGTCGCCAACCAACTGCACTAGT‑TGGTCGATACGAt                                                                                                                                                                                                                                             >  1:455943/1‑251 (MQ=255)
        ggtggGCGATGTCTGCTTCCAGTTCCCCATTTTCCTCA‑TTTATGCGCGTTAGCGAAC‑TGAAGATTTGCCGGGCAATATGGGTTTCGGAACGGCGCAATGCGCTGCCAGGTAGCAGATTACGCAACGGACGATAGTAGAGCACGCGCAGGATGTGCCGCCCCTGGCGGAAGCTGCGGCCCAGATGAGAAACCAGCATTTGCCGCACAGTCGCTTTGTCGCCAACCAACTGCACTAGT‑TGGTCGATACGATcc                                                                                                                                                                                                                                           >  1:109014/1‑251 (MQ=255)
             gCGATGTCTGCTTCCAGTTCCCCATTTTCCTCA‑TTTATGCGCGTTAGCGAAC‑TGAAGATTTGCCGGGCAATATGGGTTTCGGAACGGCGCAATGCGCTGCCAGGTAGCAGATTACGCAACGGACGATAGTAGAGCACGCGCAGGATGTGCCGCCCCTGGCGGAAGCTGCGGCCCAGATGAGAAACCAGCATTTGCCGCACAGTCGCTTTGTCGCCAACCAACTGCACTAGT‑TGGTCGATACGATCCTGCTc                                                                                                                                                                                                                                      <  1:532901/251‑1 (MQ=255)
              cGATGTCTGCTTCCAGTTCCCCATTTTCCTCA‑TTTATGCGCGTTAGCGAAC‑TGAAGATTTGCCGGGCAATATGGGTTTCGGAACGGCGCAATGCGCTGCCAGGTAGCAGATTACGCAACGGACGATAGTAGAGCACGCGCAGGATGTGCCGCCCCTGGCGGAAGCTGCGGCCCAGATGAGAAACCAGCATTTGCCGCACAGTCGCTTTGTCGCCAACCAACTGCACTAGT‑TGGTCGATACGATc                                                                                                                                                                                                                                            <  1:836074/244‑1 (MQ=255)
              cGATGTCTGCTTCCAGTTCCCCATTTTCCTCA‑TTTATGCGCGTTAGCGAAC‑TGAAGATTTGCCGGGCAATATGGGTTTCGGAACGGCGCAATGCGCTGCCAGGTAGCAGATTACGCAACGGACGATAGTAGAGCACGCGCAGGATGTGCCGCCCCTGGCGGAAGCTGCGGCCCAGATGAGAAACCAGCATTTGCCGCACAGTCGCTTTGTCGCCAACCAACTGCACTAGT‑TGGTCGATACGATc                                                                                                                                                                                                                                            >  2:836074/1‑244 (MQ=255)
                aTGTCTGCTTCCAGTTCCCCATTTTCCTCA‑TTTATGCGCGTTAGCGAAC‑TGAAGATTTGCCGGGCAATATGGGTTTCGGAACGGCGCAATGCGCTGCCAGGTAGCAGATTACGCAACGGACGATAGTAGAGCACGCGCAGGATGTGCCGCCCCTGGCGGAAGCTGCGGCCCAGATGAGAAACCAGCATTTGCCGCACAGTCGCTTTGTCGCCAACCAACTGCACTAGT‑TGGTCGATACGATCCTGCTcc                                                                                                                                                                                                                                     >  1:348492/1‑249 (MQ=255)
                  gTCTGCTTCCAGTTCCCCATGTTCCTCA‑TTTATGCGCGTTAGCGAAC‑TGAAGATTTGCCGGGCAATATGGGTTGTGGAACGGCGCAATGCGCTGCCAGGTAGCAGATTACGCAACGGACGATAGTAGAGCACGCGCAGGATGTGCCGCCCCTGGCGGAAGCTGCGGCCCAGATGAGAAACCAGCATTTGCCGCACAGTCGCTTTGTCGCCAACCAACTGCTCTAGT‑TGGTCGATACGATACTGCTCcagc                                                                                                                                                                                                                                  <  2:347480/250‑1 (MQ=255)
                    cTGCTTCCAGTTCCCCATTTTCCTCA‑TTTATGCGCGTTAGCGAAC‑TGAAGATTTGCCGGGCAATATGGGTTTCGGAACGGCGCAATGCGCTGCCAGGTAGCAGATTACGCAACGGACGATAGTAGAGCACGCGCAGGATGTGCCGCCCCTGGCGGAAGCTGCGGCCCAGATGAGAAACCAGCATTTGCCGCACAGTCGCTTTGTCGCCAACCAACTGCACTAGT‑TGGTCGATACGCTCCTGCTCCAGCAgg                                                                                                                                                                                                                               >  2:488071/1‑251 (MQ=255)
                         tccagggccccAATTTCCTTA‑TTTATGCCGGGTAGCGAAC‑TGAATATTGGGCGGGCTATATGGGTTTCGGAACGCCGCAATGCGCTGCCCGGTGACAGTCTACGCAACGGACGATAGTAGATCCATCGCAGGATGTGCCTCCCCTGGCGGAAGCTGCGGCCCAGATGAGAAACCAGCATTTGCCGCACAGTCGCTTTGTTGCCAACCAACTGCACTAGT‑TGGTCGATACGATCCTGCTCcagcatatc                                                                                                                                                                                                                             <  2:15320/241‑5 (MQ=255)
                           cAGTTCCCCATTTTCCTCA‑TTTATGCGCGTTAGCGAAC‑TGAAGATTTGCCGGGCAATATGGGTTTCGGAACGGCGCAATGCGCTGCCAGGTAGCAGATTACGCAACGGACGATAGTAGAGCACGCGCAGGATGTGCCGCCCCTGGCGGAAGCTGCGGCCCAGATGAGAAACCAGCATTTGCCGCACAGTCGCTTTGTCGCCAACCAACTGCACTAGT‑TGGTCGATACGATCCTGCTCCAGCAGGTCTTccg                                                                                                                                                                                                                        <  2:866039/251‑1 (MQ=255)
                            aGTTCCCCATTTTCCTCA‑TTTATGCGCGTTAGCGAAC‑TGAAGATTTGCCGGGCAATATGGGTTTCGGAACGGCGCAATGCGCTGCCAGGTAGCAGATTACGCAACGGACGATAGTAGAGCACGCGCAGGATGTGCCGCCCCTGGCGGAAGCTGCGGCCCAGATGAGAAACCAGCATTTGCCGCACAGTCGCTTTGTCGCCAACCAACTGCACTAGT‑TGGTCGATACGATCCTGCTCCAGCAGGTCTTccgc                                                                                                                                                                                                                       >  1:25508/1‑251 (MQ=255)
                               tCCCCATTTTCCTCA‑TTTATGCGCGTTAGCGAAC‑TGAAGATTGGCCGGGCAATATGGGTTTCGGAACGGCGCAATGCGCTGCCAGGTAGCAGATTACGCAACGGACGATAGTAGAGCACGCGCAGGATGTGCCGCCCCTGGCGGAAGCTGCGGCCCAGATGAGAAACCAGCATTTGCCGCACAGTCGCTTTGTCGCCAACCAACTGCCCTAGT‑TGGTCGATACGATCCTGCTCCAGCAGGTCTTccgccc                                                                                                                                                                                                                     <  2:223019/250‑1 (MQ=255)
                                   cATTTTCCTCA‑TTTATGCGCGTTAGCGAAC‑TGAAGATTTGCCGGGCAATATGGGTTTCGGAACGGCGCAATGCGCTGCCAGGTAGCAGATTACGCAACGGACGATAGTAGAGCACGCGCAGGATGTGCCGCCCCTGGCGGAAGCTGCGGCCCAGATGAGAAACCAGCATTTGCCGCACAGTCGCTTTGTCGCCAACCAACTGCACTAGT‑TGGTCGATACGATCCTGCTCCAGCAGGTCTTCCGCCCGCTGt                                                                                                                                                                                                                <  1:628684/251‑1 (MQ=255)
                                    aTTTTCCTCA‑TTTATGCGCGTTAGCGAAC‑TGAAGATTTGCCGGGCAATATGGGTTTCGGAACGGCGCAATGCGCTGCCAGGTAGCAGATTACGCAACGGACGATAGTAGAGCACGCGCAGGATGTGCCGCCCCTGGCGGAAGCTGCGGCCCAGATGAGAAACCAGCATTTGCCGCACAGTCGCTTTGTCGCCAACCAACTGCACTAGT‑TGGTCGATACGATCCTGCTCCAGCAGGTCTTCCGCCCGCTGtt                                                                                                                                                                                                               >  1:505838/1‑251 (MQ=255)
                                     atttCCTCA‑TTTATGCGCGTCAGCGAAC‑TGAAGATTTGCCGGGCAATATGGGTTTCGGAACGGCGCAATGCGCTGCAAGGTAGCAGATTACGCAACGGACGATAGTAGAGCACGCGCAGGATGTGCCGCCCCTGGCGGGAGCTGCGGCCCAGATGAGCAACCAGCATTTGCCGCACACTCGCTTTGTCTCCACCCAACTGCACTAGT‑TGGTCGATCCGCACCTGCTCCCGCAGGCCTTccgacagacgttg                                                                                                                                                                                                              >  2:42584/2‑241 (MQ=255)
                                        tCCTCA‑TTTATGCGCGTTAGCGAAC‑TGAAGATTTGCCGGGCAATATGGGTTTCGGAACGGCGCAATGCGCTGCCAGGTAGCAGATTACGCAACGGACGATAGTAGAGCACGCGCAGGATGTGCCGCCCCTGGCGGAAGCTGCGGCCCAGATGAGAAACCAGCATTTGCCGCACAGTCGCTTTGTCGCCAACCAACTGCACTAGT‑TGGTCGATACGATCCTGCTCCAGCAGGTCTTCCGCCCGCTGTTGCTg                                                                                                                                                                                                           >  1:114065/1‑251 (MQ=255)
                                          cTCA‑TTTATGCGCGTTAGCGAAC‑TGAAGATTTGCCGGGCAATATGGGTTTCGGAACGGCGCAATGCGCTGCCAGGTAGCAGATTACGCAACGGACGATAGTAGAGCACGCGCAGGATGTGCCGCCCCTGGCGGAAGCTGCGGCCCAGATGAGAAACCAGCATTTGCCGCACAGTCGCTTTGTCGCCAACCAACTGCACTAGT‑TGGTCGATACGATCCTGCTCCAGCAGGTCTTCCGCCCGCTGTTGCTGa                                                                                                                                                                                                          >  2:361090/1‑250 (MQ=255)
                                           ccA‑TTTATGCGCGTTAGCGAAC‑TGAAGATTTGCCGGGCAATATGGGTTTCGGAACGGCGCAATGCGCTGCCATGTAGCAGATTACGCAACGGACGATAGTAGAGCACGCGCAGGATGTGCCGCCCCTGGCGGAAGCTGCGGCCCAGATGAGAAACCAGCATTTGCCGCACAGTCGCTTTGTCGCCAACCAACTGCACTAGT‑TGGTCGATACGATCCTGCTCCAGCAGGTCTTCCGCCCGCTGTTGCTGAAg                                                                                                                                                                                                        <  2:267308/250‑1 (MQ=255)
                                             a‑TTTATGCGCGTTAGCGAAC‑TGAAGATTTGCCGGGCAATATGGGTTTCGGAACGGCGCAATGCGCTGCCAGGTAGCAGATTACGCAACGGACGATAGTAGAGCACGCGCAGGATGTGCCGCCCCTGGCGGAAGCTGCGGCCCAGATGAGAAACCAGCATTTGCCGCACAGTCGCTTTGTCGCCAACCAACTGCACTAGT‑TGGTCGATACGATCCTGCTCCAGCAGGTCTTCCGCCCGCTGTTGCTGAAgcg                                                                                                                                                                                                      <  2:342346/251‑1 (MQ=255)
                                                 tATGCGCGTTAGCGAAC‑TGAAGATTTGCCGGGCAATATGGGTTTCGGAACGGCGCAATGCGCTGCCAGGTAGCAGATTACGCAACGGACGATAGTAGAGCACGCGCAGGATGTGCCGCCCCTGGCGGAAGCTGCGGCCCAGATGAGAAACCAGCATTTGCCGCACAGTCGCTTTGTCGCCAACCAACTGCACTAGG‑TGGTCGATACGATCCTGCTCCAGCAGGTCTTCCGCCCGCTGTTGCTGAAGCGcc                                                                                                                                                                                                    <  1:418539/250‑1 (MQ=255)
                                                  aTGCGCGTTAGCGAAC‑TGAAGATTTGCCGGGCAATATGGGTTTCGGAACGGCGCAATGCGCTGCCAGGTAGCAGATTACGCAACGGACGATAGTAGAGCACGCGCAGGATGTGCCGCCCCTGGCGGAAGCTGCGGCCCAGATGAGAAACCAGCATTTGCCGCACAGTCGCTTTGTCGCCAACCAACTGCACTAGT‑TGGTCGATACGATCCTGCTCCAGCAGGTCTTCCGCCCGCTGTTGCTGAAGCGcc                                                                                                                                                                                                    >  1:322028/1‑249 (MQ=255)
                                                  aTGCGCGTTAGCGAAC‑TGAAGATTTGCCGGGCAATATGGGTTTCGGAACGGCGCAATGCGCTGCCAGGTAGCAGATTACGCAACGGACGATAGTAGAGCACGCGCAGGATGTGCCGCCCCTGGCGGAAGCTGCGGCCCAGATGAGAAACCAGCATTTGCCGCACAGTCGCTTTGTCGCCAACCAACTGCACTAGT‑TGGTCGATACGATCCTGCTCCAGCAGGTCTTCCGCCCGCTGTTGCTGAAGCGCCAg                                                                                                                                                                                                  <  1:698061/251‑1 (MQ=255)
                                                      gcgTTAGCGAAC‑TGAAGATTTGCCGTGCAATATGGGTTTCGGAACGGCGCAATGCGCTGCCAGGTAGCAGATTACGCAACGGACGATAGTAGAGCACGCGCAGGATGTGCCGCCCCTGGCGGAAGCTGCGGCCCAGATGAGAATCCAGCATTTGCCGCACAGTCGCTTTGTCGCCAACCAACTGCACTAGT‑TGGTCGATACGATCCTGCTCCAGCAGGTCTTCCGCCCGCTGTTGCTGAAGCGCCAGCCCg                                                                                                                                                                                              <  1:582494/251‑1 (MQ=255)
                                                      gcgTTAGCGAAC‑TGAAGATTTGCCGGGCAATATGGGTTTCGGAACGGCGCAATGCGCTGCCAGGTAGCAGATTACGCAACGGACGATAGTAGAGCACGCGCAGGATGTGCCGCCCCTGGCGGAAGCTGCGGCCCAGATGAGAAACCAGCATTTGCCGCACAGTCGCTTTGTCGCCAACCAACTGCACTAGT‑TGGTCGATACGATCCTGCTCCAGCAGGTCTTCCGCCCGCTGTTGCTGCAGCGCCAGCCCg                                                                                                                                                                                              >  1:385881/1‑251 (MQ=255)
                                                       cgTTAGCGAAC‑TGAAGATTTGCCGGGCAATATGGGTTCCGGAACGGCGCAATGCGCTGCCAGGTAGCAGATTACGCAACGGACGATAGTAGAGCACGCGCAGGATGTGCCGCCCCTGGCGGAAGCTGCGGCCCAGATGAGAAACCAGCATTTGCCGCACAGTCGCTTTGTCGCCAACCAACTGCACTAGT‑TGGTCGATACGATCCTGCTCCAGCAGGTCTTCCGCCCGCTGTTGCTGAAGCGCCAGCCCgg                                                                                                                                                                                             <  1:486380/251‑1 (MQ=255)
                                                                 c‑TGAAGATTTGCCGGGCAATATGGGTTTCGGAACGGCGCAATGCGCTGCCAGGTAGCAGATTACGCAACGGACGATAGTAGAGCACGCGCAGGATGTGCCGCCCCTGGCGGAAGCTGCGGCCCAGATGAGAAACCAGCATTTGCCGCACAGTCGCTTTGTCGCCAACCAACTGCACTAGT‑TGGTCGATACGATCCTGCTCCAGCAGGTCTTCCGCCCGCTGTTGCTGAAGCGCCAGCCCGGTATAGAGGa                                                                                                                                                                                    >  1:573807/1‑250 (MQ=255)
                                                                           ttccGGGCAACATGGGTTTCGGAACGGCGCAATGCGCTGCCAGGTAGCAGATTACGCAACGGACGATAGTAGAGCACGCGCAGGATGTGCCGCCCCTGGCGGAAGCTGCGGCCCAGATGAGAAACCAGCATTTGCCGCACAGTCGCTTTGTCGCCAACCAACTGCACTAGT‑TGGTCGATACGATCCTGCTCCAGCAGGTCTTCCGCCCGCTGTTGCTGAAGCGCCAGCCCGGTATAGAGGAATGTCAGAc                                                                                                                                                                           <  2:925080/248‑1 (MQ=255)
                                                                            gCCGGGCAATATGGGTTTCGGAACGGCGCAATGCGCTGCCAGGTAGCAGATTACGCAACGGACGATAGTAGAGCACGCGCAGGATGTGCCGCCCCTGGCGGAAGCTGCGGCCCAGATGAGAAACCAGCATTTGCCGCACAGTCGCTTTGTCGCCAACCAACTGCACTAGT‑TGGTCGATGCGATCCTGCTCCAGCAGGTCTTCCGCCCGCTGTTGCTGAAGCGCCAGCCCGGTATAGAGGAATGTCAGAcg                                                                                                                                                                          <  2:694642/250‑1 (MQ=255)
                                                                               gggCAATAAGGGTTTCGTAACGCCGCAATGCGCGGCCAGGGAGCAGATTACGCAACGGACGATAGTAGAGCACGCGCAGGATGTGCCGCCCCTGGCGGAAGCTGCGGCCCAGATGAGAAACCAGCATTTGCCGCACAGTCGCTTTGTCGCCAACCAACTGCACTAGT‑TGGTCGATACGATCCTGCTCCAGCAGGTCTTCCGCCCGCTGTTGCTGAAGCGCCAGCCCGGTATAGAGGAATGTCAGACGCGAg                                                                                                                                                                      <  2:218614/251‑1 (MQ=255)
                                                                                   aatatGGGTTTCGGAACGGCGCAATGCGCTGCCAGGTAGCAGATTACGCAACGGACGATAGTAGAGCACGCGCAGGATGTGCCGCCCCTGGCGGAAGCTGCGGCCCAGATGAGAAACCAGCATTTGCCGCACAGTCGCTTTGTCGCCAACCAACTGCACTAGT‑TGGTCGATACGATCCTGCTCCAGCAGGTCTTCCGCCCGCTGTTGCTGAAGCGCCAGCCCGGTATAGAGGAATGTCAGACGCGAGCGtt                                                                                                                                                                  >  2:607820/2‑251 (MQ=255)
                                                                                        gggTTTCGGAACGGCGCAATGCGCTGCCAGGTAGCAGATTACGCAACGGACGATAGTAGAGCACGCGCAGGATGTGCCGCCCCTGGCGGAAGCTGCGGCCCAGATGAGAAACCAGCATTTGCCGCACAGTCGCTTTGTCGCCAACCAACTGCACTAGT‑TGGTCGATACGATCCTGCTCCAGCAGGTCTTCCGCCCGCTGTTGCTGAAGCGCCAGCCCGGTATAGAGGAATGTCAGACGCGAGCGTTTAcca                                                                                                                                                             <  1:18330/251‑2 (MQ=255)
                                                                                         ggTTTCGGAACGGCGCAATGCGCTGCCAGGTAGCAGATTACGCAACGGACGATAGTAGAGCACGCGCAGGATGTGCCGCCCCTGGCGGAAGCTGCGGCCCAGATGAGAAACCAGCATTTGCCGCACAGTCGCTTTGTCGCCAACCAACTGCACTAGT‑TGGTCGATACGATCCTGCTCCAGCAGGTCTTCCGCCCGCTGTTGCTGAAGCGCCAGCCCGGTATAGAGGAATGTCAGACGCGAGCGTTTAccac                                                                                                                                                            <  1:449391/251‑3 (MQ=255)
                                                                                             tCGGAACTGCGCAATGCGCTGCCAGGTAGCAGATTACGCAACGGACGATAGTAGAGCACGCGCAGGATGTGCCGCCCCTGGCGGAAGCTGCGGCCCAGATGAGAAACCAGCATTTGCCGCACAGTCGCTTTGTCGCCAACCAACTGCACTAGT‑TGGTCGATACGATCCTGCTCCAGCAGGTCTTCCGCCCGCTGTTGCTGCAGCGCCAGCCCGGTATAGAGGAATGTCAGCCGCGAGCGTTTACCACGccc                                                                                                                                                        >  2:402096/1‑251 (MQ=255)
                                                                                             tCGGAACGGCGCAATGCGCTGCCAGGTAGCAGATTACGCAACGGACGATAGTAGAGCACGCGCAGGATGTGCCGCCCCTGGCGGAAGCTGCGGCCCAGATGAGAAACCAGCATTTGCCGCACAGTCGCTTTGTCGCCAACCAACTGCACTAGT‑TGGTCGATACGATCCTGCTCCAGCAGGTCTTCCGCCCGCTGTTGCTGAAGCGCCAGCCCGGTATAGAGGAATGTCAGACGCGAGCGTTTACCACGccc                                                                                                                                                        >  2:169493/1‑251 (MQ=255)
                                                                                              cGGAACGGCCCAATGCGCTGCCAGGTAGCAGATTTCGCAACGGACGATAGTAGAGCACGCGCAGGATGTTCCGCCCCTGGCGGAAGCTGCGGCCCAGATGAGAAACCAGCATTTGCCGCACAGTCGCTTTGTCGCCAACCAACTGCACTAGT‑TGGTCGATACGATCCTGCTCCAGCAGGTCTTCCGCCCGCTGTTGCTGAAGCGCCAGCCCGGTATAGAGGAATGTCAGACGCGAGCGTTTACCACGCCCg                                                                                                                                                       <  1:137380/251‑1 (MQ=255)
                                                                                               ggAACGGCGCAATGCGCTGCCAGGTAGCAGATTACGCAACGGACGATAGTAGAGCACGCGCAGGATGTGCCGCCCCTGGCGGAAGCTGCGGCCCAGATGAGAAACCAGCATTTGCCGCACAGTCGCTTTGTCGCCAACCAACTGCACTAGT‑TGGTCGATACGATCCTGCTCCAGCAGGTCTTCCGCCCGCTGTTGCTGAAGCGCCAGCCCGGTATAGAGGAATGTCAGACGCGAGCGTTTACCACGCCCgc                                                                                                                                                      >  1:646593/1‑250 (MQ=255)
                                                                                                  aCGGCGCAATGCGCTGCTAGGTAGCAGATTACGCAACGGACGATAGTAGAGCACGCGCAGGATGTGCCGCCCCTGGCGGAAGCTGCGGCCCAGATGAGAAACCAGCATTTGCCGCACAGTCGCTTTGTCGCCAACCAACTGCACTAGT‑TGGTCGATACGATCCTGCTCCAGCAGGTCTTCCGCCCGCTGTTGCTGAAGCGCCAGCCCGGTATAGAGGAATGTCAGACGCGAGCGTTTACCACGCCCGACtt                                                                                                                                                   <  2:337356/251‑1 (MQ=255)
                                                                                                   cGGCGCAATGCGCTGCCAGGTAGCAGATTACGCAACGGACGATAGTAGAGCACGCGCAGGATGTGCCGCCCCTGGCGGAAGCTGCGGCCCAGATGAGAAACCAGCATTTGCCGCACAGTCGCTTTGTCGCCAACCAACTGCACTAGT‑TGGTCGATACGATCCTGCTCCAGCAGGTCTTCCGCCCGCTGTTGCTGAAGCGCCAGCCCGGTATAGAGGAATGTCAGAc                                                                                                                                                                           <  2:769068/226‑1 (MQ=255)
                                                                                                     gcgcAATGCTCTGCCAGGTAGCAGATTACGCAACGGACGATAGTAGAGCACGCTCAGGATGTTCCGCCCCTGGCGGAAGCTGCGGCCCAGATGAGAAACCAGCATTTGCCGCACAGTCGCTTTGTCGCCAACCAACTGCACTAGT‑TGGGCGATACGATCCTGCTCCAGCAGGTCTTCCGCCCGCTGTTGCTGAAGCGCCAGCCCGGTATAGAGGAATGTCAGACGCGAGCGTTTACCACGCCCGACTTCCg                                                                                                                                                <  2:64093/251‑1 (MQ=255)
                                                                                                         aaTGCGCTGCCAGGTAGCAGATTACGCAACGGACGATAGTAGAGCACGCGCAGGATGTGCCGCCCCTGGCGGAAGCTGCGGCCCAGATGAGAAACCAGCATTTGCCGCACAGTCGCTTTGTCGCCAACCAACTGCACTAGT‑TGGTCGATACGATCCTGCTCCAGCAGGTCTTCCGCCCGCTGTTGCTGAAGCGCCAGCCCGGTATAGAGGAATGTCAGACGCGAGCGTTTACCACGCCCGACTTCCGCTTc                                                                                                                                            <  1:406206/251‑1 (MQ=255)
                                                                                                         aaTGCGCTGCCAGGTAGCAGATTACGCAACGGACGATAGTAGAGCACGCGCAGGATGTGCCGCCCCTGGCGGAAGCTGCGGCCCAGATGAGAAACCAGCATTTGCCGCACAGTCGCTTTGTCGCCAACCAACTGCACTAGT‑TGGTCGATACGATCCTGCTCCAGCAGGTCTTCCGCCCGCTGTTGCTGAAGCGCCAGCCCGGTATAGAGGAATGGCAGACGCGAGCGTTTACCACGCCCGACTTCCGCTTc                                                                                                                                            >  2:373769/1‑251 (MQ=255)
                                                                                                               cTGCCAGGTAGCAGATTACGCAACGGACGATAGTAGAGCACGCGCAGGATGTGCCGCCCCTGGCGGAAGCTGCGGCCCAGATGAGAAACCAGCATTTGCCGCACAGTCGCTTTGTCGCCAACCAACTGCACTAGT‑TGGTCGATACGATCCTGCTCCAGCAGGTCTTCCGCCCGCTGTTGCTGAAGCGCCAGCCCGGTATAGAGGAATGTCAGACGCGAGCGTTTACCACGCCCGACTTCCGCTTCCCACGt                                                                                                                                      >  1:104923/1‑251 (MQ=255)
                                                                                                               cTGCCAGGTAGCAGATTACGCAACGGACGATAGTAGAGCACGCGCAGGATGTGCCGCCCCTAGCGGAAGCTGCGGCCCAGATGAGAAACCAGCATTTGCCGCACAGTCGCTTTGTCGCCAACCAACTGCACTAGT‑TGGTCGATACGATCCTGCTCCAGCAGGTCTTCCGCCCGCTGTTGCTGAAGCGCCAGCCCGGTATAGAGGAATGTCAGACGCGAGCGTTTACCACGCCCGACTTCCGCTTCCCACgc                                                                                                                                      >  2:273272/1‑250 (MQ=255)
                                                                                                                tGCCAGGTAGCAGATTACGCAACGGACGATAGTAGAGCACGCGCAGGATGTGCCGCCCCTGGCGGAAGCTGCGGCCCAGATGAGAAACCAGCATTTGCCGCACAGTCGCTTTGTCGCCAACCAACTGCACTAGT‑TGGTCGATACGATCCTGCTCCAGCAGGTCTTCCGCCCGCTGTTGCTGAAGCGCCAGCCCGGTATAGAGGAATGTCAGACGCGAGCGTTTACCACGCCCGACTTCCGCTTCCCACGTc                                                                                                                                     >  1:133373/1‑251 (MQ=255)
                                                                                                                 gCCAGGTAGCAGATTACGCAACGGACGATAGTAGAGCACGCGCAGGATGTGCCGCCCCTGGCGGAAGCTGCGGCCCAGATGAGAAACCAGCATTTGCCGCACAGTCGCTTTGTCGCCAACCAACTGCACTAGT‑TGGTCGATACGATCCTGCTCCAGCAGGTCTTCCGCCCGCTGTTGCTGAAGCGCCAGCCCGGTATAGAGGAATGTCAGACGCGAGCGTTTACCACGCCCGACTTCCGCTTCCCACGt                                                                                                                                      <  1:828176/249‑1 (MQ=255)
                                                                                                                           aGATTACGCAACGGACGATAGTAGAGCACGCGCAGGATGTGCCGCCCCTGGCGGAAGCTGCGGCCCAGATGAGAAACCAGCATTTGCCGCACAGTCGCTTTGTCGCCAACCAACTGCACTAGT‑TGGTCGATACGATCCTGCTCCAGCAGGTCTTCCGCCCGCTGTTGCTGAAGCGCCAGCCCGGTATAGAGGAATGTCAGACGCGAGCGTTTACCACGCCCGACTTCCGCTTCCCACGTCAGCCAGCcgcg                                                                                                                          >  1:587833/1‑251 (MQ=255)
                                                                                                                                              agtagAGAACGCGAAGGATGTGCCGCCCCTGGCGGAAGCTGCGGCCCAGATGAGACACCAAAATTTGCCGCACAGTCGCTTTGTCGCCAACCAACTGCACTAGT‑TGGTCGATACGATCCTGCTCCAGCAGGTCTTCCGCCCGCTGTTGCTGACACGCCAGCCAGGGATTGAGGCATGCCAGCCACGCGCGTTTACCACGACCGGCTTCCGCTTCACACGTCTTCCAGCCGCGATCCTGCATGGTGTTGAGc                                                                                                       >  2:941340/1‑251 (MQ=255)
                                                                                                                                                   agCACGCGCAGGATGTGCCGCCCCTGGCGGAAGCTGCGGCCCAGATGAGAAACCAGCATTTGCCGCACAGTCGCTTTGTCGCCAACCAACTGCACTAGT‑TGGTCGATACGATCCTGCTCCAGCAGGTCTTCCGCCCGCTGTTGCTGAAGCGCCAGCCCGGTATAGAGGAATGTCAGACGCGAGCGTTTACCACGCCCGACTTCCGCTTCCCACGTCAGCCAGCCGCGATCCTGCATGGTGTTGAGCAGGGt                                                                                                  <  1:804167/251‑1 (MQ=255)
                                                                                                                                                       cgcgCAGGATGTGCCGCCCCTGGCGGAAGCTGCGGCCCAGATGAGAAACCAGCATTTGCCGCACAGTCGCTTTGTCGCCAACCAACTGCACTAGT‑TGGTCGATACGATCCTGCTCCAGCAGGTCTTCCGCCCGCTGTTGCTGAAGCGCCAGCCCGGTATAGAGGAATGTCAGACGCGAGCGTTTACCACGCCCGACTTCCGCTTCCCACGTCAGCCAGCCGCGATCCTGCATGGTGTTGAGCAGGGTgcgc                                                                                              <  1:26607/251‑1 (MQ=255)
                                                                                                                                                         cgaaGGATGTGCCGCCCCTGGCGGAAGCTGCGGCCCAGATGATAAGCCAGCATGTGCCGCACAGTCGCTTTGTCGCCAACGAACTGCTCTAGG‑TGGTCGATACGCTCCTGCTCCAGCAGGTCTTCCGCCCTCTGTTGCTGAAGCGCCAGCCCGGTATAGAGGAATGGCAGACGCGAGCGTGTACCCCGCCCGACTTCCGCTTCCCACGTCAGCCAGCCGCGATCCTGCATGGTGTTGAGCAGGGGGCGCat                                                                                            <  2:380809/248‑1 (MQ=255)
                                                                                                                                                            aGGATGTGACGCCCCTGGCGGAAGCTGCGGCCCAGATGAGAAACCAGCATTTGCCGCACAGTCGCTTTGTCGCCAACCAACTGCACTAGT‑TGGTCGATACGATCCTGCTCCAGCAGGTCTTCCTCCCGCTGTTGCTGAAGCGCCAGCCCGGTATAGAGGAATGTCAGACGCGAGCGTTTACCACGCCCGACTTCCGCTTCCCACGTCATCCAGCCGCGAGCCTGCATGGTGTTGAGCAGGGTGCGCATATg                                                                                         <  1:746383/251‑1 (MQ=255)
                                                                                                                                                             ggaggtgCCGCCCCTGGCGGAAGCTGCGGCCCAGATGAGAAACCAGCATTTGCCGCACAGTCGCTTTGTCGCCAACCAACTGCACTAGT‑TGGTCGATACGATCCTGCTCCAGCAGGTCTTCCGCCCGCTGTTGCTGAAGCGCCAGCCCGGTATAGAGGAATGTCAGACGCGAGCGTTTACCACGCCCGACTTCCGCTGCCCACGTCAGCCAGCCGCGATCCTGCATGGTGTTGAGCAGGGTGCGCATATga                                                                                        <  2:468154/247‑1 (MQ=255)
                                                                                                                                                             ggATGTGCCGCCCCTGGCGGAAGCTGCGGCCCAGATGAGAAACCAGCATTTGCCGCACAGTCGCTTTGTCGCCAACCAACTGCACTAGT‑TGGTCGATACGATCCTGCTCCAGCAGGTCTTCCGCCCGCTGTTGCTGAAGCGCCAGCCCGGTATAGAGGAATGTCAGACGCGAGCGTTTACCACGCCCGACTTCCGCTTCCCACGTCAGCCAGCCGCGATCCTGCATGGTGTTGAGCAGGGTGCGCATATga                                                                                        <  1:941281/251‑1 (MQ=255)
                                                                                                                                                                        cccTGGCGGAAGCTGCGGCCCAGATGAGAACCCAGCATTGGCCGCACATGCGCTTTGTCGCCAACCAACTGCACTAGT‑TGGTCGATACGATCCGGCTCCAGCAGGTCTTCCGCCCGCTGGTGCTGAAGCGCCAGCCCGGTATAGAGGAATGGCAGACGCGAGCGTTTACCACGCCCGACTTCCGCTTCCCACGTCAGCCAGCCGCGATCCTGCATGGTGTTGAGCAGGGTGCGCATATGACGACGCGagca                                                                             <  2:562037/251‑1 (MQ=255)
                                                                                                                                                                        cccTGGCGGAAGCTGCGGCCCAGATGAGAAACCAGCATTTGCCGCACAGTCGCTTTGTCGCCAACCAACTGCACTAGT‑TGGTCGATACGATCCTGCTCCAGCAGGTCTTCCGCCCGCTGTTGCTGAAGCGCCAGCCCGGTATAGAGGAATGTCAGACGCGCGCGTTTACCACGCCCGACTTCCGCTTCCCACGTCAGCCAGCCGCGATCCTGCATGGTGTTGAGCAGGGTGCGCATATGACGCCGCGaccc                                                                             >  2:155937/1‑248 (MQ=255)
                                                                                                                                                                        cccTGGCGGAAGCTGCGGCCCAGATGAGAAACCAGCATTTGCCGCACAGTCGCTTTGTCGCCAACCAACTGCACTAGT‑TGGTCGATACGATCCTGCTCCAGCAGGTCTTCCGCCCGCTGTTGCTGAAGCGCCAGCCCGGTATAGAGGAATGTCAGACGCGAGCGTTTACCACGCCCGACTTCCGCTTCCCACGTCAGCCAGCCGCGATCCTGCATGGTGTTGAGCAGGGTGCGCATATGACGACGCGagc                                                                              >  1:908857/1‑250 (MQ=255)
                                                                                                                                                                           gggCGGAAGCTGCGGCTCAGATGAGAAACCAGCATTTGCCGCACAGTCGCTTTGGCGCCAACCAACTGCCCTAGT‑TGGTCGATACGATCCTTCTCCAGCAGGTCTTCCGCCCGCTGTTGCTGAAGCGCCAGCCCGGTATAGAGGAATGTCAGACGCGAGCGTTTACCACGCCCGACTTCCGCTTCCCACGTCAGCCAGCCGCGATCCTGCATGGTGTTGAGCAGGGTGCGCATATGACGACGCGAGCAGCt                                                                          <  2:902322/250‑1 (MQ=255)
                                                                                                                                                                                    cTGCGGCCCAGATGAGAAACCAGCATTTGCCGCACAGTCGCTTTGTCGCCAACCAACTGCACTAGT‑TGGTCGATACGATCCTGCTCCAGCAGGTCTTCCGCCCGCTGTTGCTGAAGCGCCAGCCCGGTATAGAGGAATGTCAGACGCGAGCGTTTACCACGCCCGACTTCCGCTTCCCACGTCAGCCAGCCGCGATCCTGCATGGTGTTGAGCAGGGTGCGCATATGACGACGCGAGCAGCTCAATAACGc                                                                 >  2:6758/1‑251 (MQ=255)
                                                                                                                                                                                      gcgtcccAGATGAGAAACCAGCATTTGCCGCACAGTCGCTTTGTCGCCAACCAACTGCACTAGT‑TGGTCGATACGATCCTGCTCCAGCAGGTCTTCCGCCCGCTGTTGCTGAAGCGCCAGCCCGGTATAGAGGAATGTCAGACGCGAGCGTTTACCACGCCCGACTTCCGCTTCCCACGTCAGCCAGCCGCGATCCTGCATGGTGTTGAGCAGGGTGCGCATATGACGACGCGAGCAGCTCAATAACGCt                                                                <  1:718962/246‑1 (MQ=255)
                                                                                                                                                                                       gggCCCAGATGAGAAACCAGCATTTGCAGCACAGTCGCTTTGTCGCCAACCAACTGGACTAGT‑TGGTCGATACGATCCGGCTCCAGCAGGTCTTCCGCCCGCTGTTGCGGAAGCGCCAGCCCGGTATAGAGGAATGTCAGACGCGAGCGTTTACCACGCCCGACTTCCGCTTCCCACGTCAGCCAGCCGCGATCCTGCATGGTGTTGAGCAGGGTGCGCATATGACGACGCGAGCAGCTCAATAACGCTGc                                                              <  2:673125/250‑1 (MQ=255)
                                                                                                                                                                                          cccAGATGAGAAACCAGCATTTGCCGCACAGTCGCTTTGTCGCCAACCAACTGCACTAGT‑TGGTCGATACGATCCTGCTCCAGCAGGTCTTCCGCCCGCTGTTGCTGAAGCGCCAGCCCGGTATAGAGGAATGTCAGACGCGAGCGTTTACCACGCCCGACTTCCGCTTCCCACGTCAGCCAGCCGCGATCCTGCATGGTGTTGCGCAGGGTGCGCATATGACGACGCGAGCAGCTCAATAACGCTGcc                                                             >  2:775094/1‑249 (MQ=255)
                                                                                                                                                                                           ccAGATGAGAAACCAGCATTTGCCGCACAGTCGCTATGTCGCCAACCAACTGCACTAGT‑TGGTCGATACGATCCTGCTCCCGCAGGTCTTCCGCCCGCTGTTGCTAACACGCCAGCCCGGCATAGAGGAATGTCAGACGCGAGCGTTTACCACGCACGACTTCCGCTTCCCACGTCAGCCAGCCGCGATCCTGCATGATGTTGAGCAGGGTGCGCGTATTACGACGCGAGCAGCACAATAAAGCTGCCAGt                                                          >  2:862065/1‑251 (MQ=255)
                                                                                                                                                                                            cAGATGAGAAACCAGCATTTGCCGCACAGTCGCTTTGTCGCCAACCAACTGCACTAGT‑TGGTCGATACGATCCTGCTCCAGCAGGTCTTCCGCCCGCTGTTGCTGAAGCGCCAGCCCGGTATAGAGGAATGTCAGACGCGAGCGTTTACCACGCCCGACTTCCGCTTCCCACGTCAGCCAGCCGCGATCCTGCATGGTGTTGAGCAGGGTGCGCATATGACGACGCGAGCAGCTCAATAACGCTGCCAGtt                                                         <  1:567190/251‑1 (MQ=255)
                                                                                                                                                                                             agacgagaAACCAGCATTTGCCGCACAGTCGCTTTGTCGCCAACCAACTGCACTAGT‑TTGTCGATACGATCCTGCTCCAGCAGGTCCTCCGCCCGCTGTTGCTGAAGCGCCAGCCCTGTATAGAGGAATGTCAGACGCGATCGGTTACCACGCCCGACTTCCGCTTCCCACGTCAGCCAGCCGCGATCCGGCATGGTGTTGAGCAGGGTGTGCATAGGACGACGCGAGCAGCTCAATAACGCTGCCAGTTc                                                        <  2:926085/247‑1 (MQ=255)
                                                                                                                                                                                             aGATGAGAAACCAGCATTTGCCGCACAGTCGCTTTGTCGCCAACCAACTGCACTAGT‑TGGTCGATACGATCCTGCTCCAGCAGGTCTTCCGCCCGCTGTTGCTGAAGCGCCAGCCCGGTATAGAGGAATGTCAGACGCGAGCGTTTACCACGCCCGACTTCCGCTTCCCACGTCAGCCAGCCGCGATCCTGCATGGTGTTGAGCAGGGTGCGCATATGACGACGCGAGCAGCTCAATAACGCTGCCAGTTc                                                        >  1:744630/1‑251 (MQ=255)
                                                                                                                                                                                              gaggagaAACAAGAATTTGCCGCACATGCGCTTTTGCGCCACACACCTGCCCTAGT‑GGGTGGATACGATCCTGCTCCAGCAGGTCTTCCGCCCGCTGTTGCTGAAGCGCCATCCCGGTATAGAGGAATGTCAGACGCGAGCGTTTACCACGCCCGACTTCCGATTCCCACGTCAGCCAGCCGCGATCCTGCATGGTGTTGAGCAGGGTGCGCATATGACGACGCGAGCAGCGCAATAACGCTGCCAGTTCg                                                       <  2:883324/248‑1 (MQ=255)
                                                                                                                                                                                              gATGAGAAACCAGCATTTGCCGCACAGTCGCTTTGTCGCCAACCAACTGCACTAGT‑TGGTCGATACGATCCTGCTCCAGCAGGTCTTCCGCCCGCTGTTGCTGAAGCGCCAGCCCGGTATAGAGGAATGTCAGACGCGAGCGTTTACCACGCCCGACTTCCGCTTCCCACGTCAGCCAGCCGCGATCCTGCATGGTGTTGAGCAGGGTGCGCATATGACGACGCGCGCAGCTCAATAACGCTGCCAGTTCg                                                       >  1:661273/1‑251 (MQ=255)
                                                                                                                                                                                                tGAGAAACCAGCATTTGCCGCACAGTCGCTTTGTCGCCAACCAACTGCACTAGT‑TGGTCGATACGATCCTGCTCCAGCAGGTCTTCCGCCCGCTGTTGCTGAAGCGCCAGCCCGGTATAGAGGAATGTCAGACGCGAGCGTTTACCACGCCCGACTTCCGCTTCCCACGTCAGCCAGCCGCGATCCTGCATGGTGTTGAGCAGGGTGCGCATATGACGACGCGAGCAGCTCAATAACGCTGCCAGTTCGtt                                                     >  1:474354/1‑251 (MQ=255)
                                                                                                                                                                                                            aTTTGCCGCACAGTCGCTTTGTCGCCAACCAACTGCACTAGT‑TGGTCGATACGATCCTGCTCCAGCAGGTCTTCCGCCCGCTGTTGCTGAAGCGCCAGCCCGGTATAGAGGAATGTCAGACGCGAGCGTTTACCACGCCCGACTTCCGCTTCCCACGTCAGCCAGCCGCGATCCTGCATGGTGTTGAGCAGGGTGCGCATATGACGACGCGCGCAGCTCAATAACGCTGCCCGTTCGTTGGGCGTTGTGTc                                         >  2:800692/1‑251 (MQ=255)
                                                                                                                                                                                                            aTTTGACGCACATTCGCTTTGTCGCCAACAAACTGCACTAGT‑TGGTCGATACGCTCCTGCTCCAGCAGGACTTCCGCCCGCTGTTGCTGAAGCGCCAGCCCGGTATAGAGGAATGTCAGACGCGCGCGTTTACCACGCCCGACTTCCGCTTCCAACGTCAGCCAGCCGCGATCCGGCATGGTGTTGGGCAGGGTGCGAATAAGCAGACGCGAGCAGCCCACTAAAGCTGccgtatagtgcaacgttgggtc                                         >  2:953790/1‑231 (MQ=255)
                                                                                                                                                                                                              ttGCCTCAAAGTCTCTTTGTCGCCACCCAACTGCCCGAGT‑GGGACGGTACTATCCTCCCTCAGCAGGTATTCCGCCCGCTGTTGCTGAAGCGCCAGCCCGGTATAGAGGAATGTCAGACGCGAGCGTTTACCACGCCCGACTTCCGCTTCCCACGTCAGCCAGCCGCGAGCCTGCATGGTGTTGAGCAGGGTGCGCATATGACGACGCGAGCAGCTCAATAACGCTGCCAGTTCGTTGAGCGTTGTGTcc                                        <  2:738836/250‑1 (MQ=255)
                                                                                                                                                                                                               tGCCGCCCCGTCGCTTTGTCGCCAACCAACTGCACTAGT‑TGGTCGATACGATCCTGCTCCAGCAGGTCTGCCGCCCGCTGTTGCTGAAGCGCCAGCCCGGTATAGAGGAATGTCAGACGCGAGCGTTTACCACGCCCGACTTCCGCTTCCCACGTCAGCCAGCCGCGATCCTGCATGGTGTTGAGCAGGGTGCGCATATGACGACGCGAGCAGCTCAATAACGCTGCCAGTTCGTTGAGCGTTGTGTCCt                                       <  2:738718/250‑1 (MQ=255)
                                                                                                                                                                                                               tGCCGCACAGTCGCTTTGTCGCCAACCAACTGCACTAGT‑TGGTCGATACGATCCTGCTCCAGCAGGTCTTCCGCCCGCTGTTGCTGAAGCGCCAGCCCGGTATAGAGGAATGTCAGACGCGAGCGTTTACCACGCCCGACTTCCGCTTCCCACGTCAGCCAGCCGCGATCCTGCATGGTGTTGAGCAGGGTGCGCATATGACGACGCGAGCAGCTCAATAACGCTGCCAGTTCGTTGAGCGTTGTGTCCt                                       <  2:437527/250‑1 (MQ=255)
                                                                                                                                                                                                                gCCGCACAGTCGCTTTGTCGCCAACCAACTGCACTAGT‑TGGTCGATACGATCCTGCTCCAGCAGGTCTTCCGCCCGCTGTTGCTGAAGCGCCAGCCCGGTATAGAGGAATGTCAGACGCGAGCGTTTACCACGCCCGACTTCCGCTTCCCACGTCAGCCAGCCGCGATCCTGCATGGTGTTGAGCAGGGTGCGCATATGACGACGCGAGCCGCTCAATAACGCTGCCAGTTCGTTGAGCGTTGTGTCCTGc                                     >  2:490516/1‑251 (MQ=255)
                                                                                                                                                                                                                     ccaggcGCTTTGTCGCCAACCAACTGCCCAAGT‑TGGTCGATACGATCCTGCTCCAGCAGGTCTTCCGCCCGCTGTTGCTGAAGCGCCAGCCCGGTATAGAGGAAGGTCAGACGCGAGCGGTTACCACGCCCGACTTCCGCTTCCCACGTCAGCCAGCCGCGATCCTGCATGGTGTTGAGCAGGGTGCGCATATGACGACGCGAGCAGCTCAATAACGCTGCCAGTTCGTTGAGCGTTGTGTCCTGCGAttt                                <  2:86172/246‑1 (MQ=255)
                                                                                                                                                                                                                     acaGTCGCTTTGTCGCCAACCAACTGCACTAGT‑TGGTCGATACGATCCTGCTCCAGCAGGTCTTCCGCCCGCTGTTGCTGAAGCGCCAGCCCGGTATAGAGGAATGTCAGACGCGAGCGTTTACCACGCCCGACTTCCGCTTCCCACGTCAGCCAGCCGCGATCCTGCATGGTGTTGAGCAGGGTGCGCATATGACGACGCGAGCAGCTCAATAACGCTGCCAGTTCGTTGAGCGTTGTGTCCTGCGAttt                                >  1:706186/1‑251 (MQ=255)
                                                                                                                                                                                                                       aggtgCTTTGTCGCCAACCAACTGCAA‑TGTGTGGTCGATACGTTCCGGCTCCAGCAGGGCTTCCTCCCGCTGTTGCTGAAGCGCCAGCCCGGTATAGAGGAATGTCAGACGCGAGCGGTTACCACGCCCGACTTCCGCTTCCCACGTCAGCCAGCCGCGATCCTGCATGGTGTGGAGCAGGGTGCGCATATGACGTCGCGAGCAGCTCAATAACGCTGCCAGTTCGTTTAGCGTTGTGTCCTGCGATTTAc                              <  2:627199/247‑1 (MQ=255)
                                                                                                                                                                                                                       aGTCGCTTTGTCGCCAACCAACTGCACTAGT‑TGGTCGATACGATCCTGCTCCAGCAGGTCTTCCGCCCGCTGTTGCTGAAGCGCCAGCCCGGTATAGAGGAATGTCAGACGCGAGCGTTTACCACGCCCGACTTCCGCTTCCCACGTCAGCCAGCCGCGATCCTGCATGGTGTTGAGCAGGGTGCGCATATGACGACGCGAGCAGCTCAATAACGCTGCCAGTTCGTTGAGCGTTGTGTCCTGCGATTTAc                              >  1:623819/1‑251 (MQ=255)
                                                                                                                                                                                                                        gTCGCTTTGTCGCCAACCAACTGCACTAGT‑TGGGCGATACGATCCTGCTCCAGCAGGTCTTCCGCCCGCTGTTGCTGAAGCGCCAGCCCGGTATAGAGGAATGTCAGACGCGAGCGTTTACCACGCCCGACTTCCGCTTCCCACGTCAGCCAGCCGCGATCCTGCATGGTGTTGAGCAGGGTGCGCATATGACGACGCGAGCAGCTCAATAACGCTGCCAGTTCGTTGAGCGTTGTGTCCTGCGATTTAcc                             <  2:505838/251‑1 (MQ=255)
                                                                                                                                                                                                                               tGTCGCCAACGAACTGCACTAGT‑TGGTCGATACGATCCTGCTCCAGCAGGTCTTCCGCCCGCTGTTGCTGATGCGCCAGCCCGGTATAGAGGAATGTCAGACGCGAGCGTTTACCACGCCCGACTTCCGCTTCCCACGTCAGCCAGCCGCGATCCTGCATGGTGTTGAGCAGGGTGCGCATATGACGACGCGAGCAGCTCAATAACGCTGCCAGTTCGTTGAGCGTTGTGTCCTGCGATTTACCCTCgca                       <  1:319467/250‑1 (MQ=255)
                                                                                                                                                                                                                               tGTCGCCAACCAACTGCACTAGT‑TGGTCGATACGATCCTGCTCCAGCAGGTCTTCCGCCCGCTGTTGCTGAAGCGCCAGCCCGGTATAGAGGAATGTCAGACGCGAGCGTTTACCACGCCCGACTTCCGCTTCCCACGTCAGCCAGCCGCGATCCTGCATGGTGTTGAGCAGGGTGCGCATATGACGACGCGAGCAGCTCAATAACGCTGCCAGTTCGTTGAGCGTTGTGTCCTGCGATTTACCCTCgca                       <  1:222553/250‑1 (MQ=255)
                                                                                                                                                                                                                               tGTCACCAACCAACTGCACTAGA‑TGGTCGATACGATCCTGCTCCAGCAGGTCTTCCGCCCGCTGTTGCTGAAGCGCCAGCCCGGTATAGAGGAATGTCAGACGCGAGCGTTTACCACGCCCGACTTCCGCTACACACGTCAGCCCGCCGCGATCCTGCCTGGTGTTGCGCAGGGTGCGCATATGACGCCGCGAGCAGCTCACAAACGCTGCCCGTTCATTGAGCGTTGTGTCCTGCGATTTACCCCCgccg                      >  2:869568/1‑249 (MQ=255)
                                                                                                                                                                                                                                gTCGCCAACCAACTGCACTAGT‑TGGTCGATACGATCCTGCTCCAGCAGGTCTTCCGCCCGCTGTTGCTGAAGCGCCAGCCCGGTATAGAGGAATGTCAGACGCGAGCGTTTACCACGCCCGACTTCCGCTTCCCACGTCAGCCAGCCGCGATCCTGCATGGTGTTGAGCAGGGTGCGCATATGACGACGCGAGCAGCTCAATAACGCTGCCAGTTCGTTGAGCGTTGTGTCCTGCGATTTACCCTCgcag                      <  1:642013/250‑1 (MQ=255)
                                                                                                                                                                                                                                gTCGCCAACCAACTGCACTAGT‑TGGTCGATACGATCCTGCTCCAGCAGGTCTTCCGCCCGCTGTTGCTGAAGCGCCAGCCCGGTATAGAGGAATGTCAGACGCGAGCGTTTACCACGCCCGACTTCAGCTTCCCACGTCAGCCAGCCGCGATCCTGCATGGTGTTGAGCAGGGTGCGCATATGACGACGCGAGCAGCTCAATAACGCTGCCAGTTCGTTGAGCGTTGTGTCCTGCGATTTACCCTCgcag                      <  1:168714/250‑1 (MQ=255)
                                                                                                                                                                                                                                  cGCCAACCATCGGCACTAGT‑TGGTCTAGTCGATCCTGCTCCAGCAGGTCTTCCGCCCGCTGTTGCTGAAGCGCCAGCCCGGTATAGAGGAATGTCAGACGCGAGCGTTTACCACGCCCGTCTTCCGCTTCCCACGTCAGCCAGCCGCGATCCTGCATGGTGTTGAGCAGGGTGCGCATATGACGACGCGAGCAGCTCAATAACGCTGCCAGTTCGTTGAGCGTTGTGTCCTGCGATTTACCCTCGCAGCAt                   <  2:424275/251‑1 (MQ=255)
                                                                                                                                                                                                                                  cGCCAACCAACTGCACTAGT‑TGGTCGATACGATCCTGCTCCAGCAGGTCTTCCGCCCGCTGTTGCTGAAGCGCCAGCCCGGTATAGAGGAATGTCAGACGCGAGCGTTTACCACGCCCGACTTCCGCTTCCCACGTCAGCCAGCCGCGATCCTGCATGGTGTTGAGCAGGGTGCGCATATGACGACGCGAGCAGCTCAATAACGCTGCCAGTTCGTTGAGCGTTGTGTCCTGCGATTTACCCTCGCAGCAt                   >  1:782349/1‑251 (MQ=255)
                                                                                                                                                                                                                                  cGCCAACCAACTGCACTAGT‑TGGTCGATACGATCCTGCTCCAGCAGGTCTTCCGCCCGCTGTTGCTGAAGCGCCAGCCCGGTATAGAGGAATGTCAGACGCGAGCGTTTACCACGCCCGACTTCCGCTTCCCACGTCAGCCAGCCGCGATCCTGCAAGGTGTTGAGCAGGGTGCGCATATGACGACGCGAGCAGCTCAATAACGCTGCCAGTTCGTTGAGCGTTGTGTCCTGCGATTTACCCTCGCAGCAt                   <  2:401090/251‑1 (MQ=255)
                                                                                                                                                                                                                                   gCCAACCAGCTGCACTAGT‑TGGCTGATCCGATCCTGTTCCAGCAGGTTTTCCTCCCGGTGCTGCTGATGCGCCAGCGCGGTATAGAGGAATGTCAGACGCGAGCGTTGACCACGCCCGACTTCCGCTTCCCACGTCAGTCAGCCGCGAGCCGGCATGGTGTTGAGCAGGGTGCGCATATGACGACGCGAGCAGCTCAATAACGCTGCCAGTTCGTTGAGCGTTGTGTCCTGCGATTTACCCTCTTAGCAtt                  <  2:421743/251‑1 (MQ=255)
                                                                                                                                                                                                                                   gCCAACCAACTGCACTAGT‑TGGTCGATACGATCCTGCTCCAGCAGGTCTTCCGCCCGCTGGTGCTGAAGCGCCAGCCCGGTATAGAGTAATGTCAGACGCGAGCGGTTACCACGCCCGACTTCCGCTTCCCACGTCAGCCAGCCGCGATCCTGCATGGTGTTGAGCAGGGTGCGCATATGACGACGCGAGCAGCTCAATAACGCTGCCAGTTCGTTGAGCGTTGTGTCCTGCGATTTACCCTCGCAGCAtt                  >  1:514658/1‑251 (MQ=255)
                                                                                                                                                                                                                                        ccaacTGCACTAGT‑TGGTCGATACGATCCTGCTCCAGCAGGTCTTCCGCCCGCTGTTGCTGAAGCGCCAGCCCGGTATAGAGGAATGTCAGACGCGAGCGTTTACCACGCCCGACTTCCGCTTCCCACGTCAGCCAGCCGCGATCCTGCATGGTGTTGAGCAGGGTGCGCATATGACGACGCGAGCAGCTCAATAACGCTGCCAGTTCGTTGAGAGTTGTGTCCTGCGATTTACCCTCGCAGCATTGCcac             >  1:150133/1‑251 (MQ=255)
                                                                                                                                                                                                                                         caacTGCACTAGT‑TGGTCGATACGATCCTGCTCCAGCAGGTCTTCCGCCCGCTGTTGCTGAAGAGCCAGCCCGGTATAGAGGAATGTCAGACGCGAGCGTTTACCACGCCCGACTTCCGCTTCCCACGTCAGCCAGCCGCGATCCTGCATGGTGTTGAGCCGGGTGCGCATATGACGCCCCGAGCAGCTCAATAACGCTGCCAGTTCGTTGAGCGTTGTGTCCTTCGATTTACCCTCGCAGCATTGCcac             >  2:980568/1‑250 (MQ=255)
                                                                                                                                                                                                                                          aacTGCACTAGT‑TGGTCGATACGATCCTGCTCCAGCAGGTCTTCCGCCCGCTGTTGCTGAAGCGCCAGCCCGGTATAGAGGAATGTCAGACGCGAGCGTTTACCACGCCCGACTTCCGCTTCCCACGTCAGCCAGCCGCGATCCTGCATGGTGTTGAACAGGGTGCGCATATGACGACGCGAGCAGCTCAATAACGCTGCCAGTTCGTTGAGCGTTGTGTCCTGCGATTTACCCTCGCAGCATTGCcac             >  1:804253/1‑249 (MQ=255)
                                                                                                                                                                                                                                           acTGCACTAGT‑TGGTCGATACGATCCTGCTCCAGCAGGTCTTCCGCCCGCTGTTGCTGAAGCGCCAGCCCGGTATAGAGGAATGTCAGACGCGAGCGTTTACCACGCCCGACTTCCGCTTCCCACGTCAGCCAGCCGCGATCCTGCATGGTGTTGAGCAGGGTGCGCATATGACGACGCGAGCAGCTCAATAACGCTGCCAGTTCGTTGAGCGTTGTGTCCTGCGATTTACCCTCGCAGCATTGCCACAgg          <  1:299879/251‑1 (MQ=255)
                                                                                                                                                                                                                                           acTGCACTAGT‑TGGTCGATACGATCCTGCTCCAGCAGGTCTTCCGCCCGCTGTTGCTGAAGCGCCAGCCCGGTATAGAGGAATGTCAGACGCGAGCGTTTACCACGCCCGACTTCCGCTTCCCACGTCAGCCAGCCGCGATCCTGCATGGTGTTGAGCAGGGTGCGCATATGACGACGCGAGCAGCTCAATAACGCTGCCAGTTCGTTGAGCGTTGTGTCCTGCGATTTACCCTCGCAGCATTGCCACAgg          >  1:581532/1‑251 (MQ=255)
                                                                                                                                                                                                                                               cactaGT‑TGGTCGATACGATCCTTGTCAAGCAGGTCTTCCGCCCGCTGTTGCTGAAGCGTCAGCCCGGTATAGAGGAATGTCAGACGCGATCGTTTACCTCGCCCGACTGCCGCTTCCCACGTCAGCCAGCCGTGATCCTGCATGGTGTTGAGCAGGGTGCGCATATGACGACGCGAGCAGCTCAATAACGCTGCGAGTTCGTTGATCGTTGTGTCCTGCGATTTGCCCTCGCAGCAGTGCCACATGCGGa      <  2:485007/247‑1 (MQ=255)
                                                                                                                                                                                                                                                   aGT‑TGGTCGATACGATCCTGCTCCAGCAGGTCTTCCGCCCGCTGTTGCTGAAGCGCCAGCCCGGTATAGAGGAATGTCAGACGCGAGCGTTTACCACGCCCGACTTCCGCTTCCCACGTCAGCCAGCCGCGATCCTGCATGGTGTTGAGCAGGGTGCGCATATGACGACGCGAGCAGCTCAATAACGCTGCCAGTTCGTTGAGCGTTGTGTCCTGCGATTTACCCTCGCAGCATTGCCACAGGCGGATGaa  <  1:169493/251‑1 (MQ=255)
                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                     
CTGCCAGTGGTGGGCGATGTCTGCTTCCAGTTCCCCATTTTCCTCA‑TTTATGCGCGTTAGCGAAC‑TGAAGATTTGCCGGGCGATATGGGTTTCGGAACGGCGCAATGCGCTGCCAGGTAGCAGATTACGCAACGGACGATAGTAGAGCACGCGCAGGATGTGCCGCCCCTGGCGGAAGCTGCGGCCCAGATGAGAAACCAGCATTTGCCGCACAGTCGCTTTGTCGCCAACCAACTGCACCAGT‑TGATCGATACGATCCTGCTCCAGCAGGTCTTCCGCCCGCTGTTGCTGAAGCGCCAGCCCGGTATAGAGGAATGTCAGACGCGAGCGTTTACCGCGCCCGACTTCCGCTTCCCACGTCAGCCAGCCGCGATCCTGCATGGTGTTGAGCAGGGTGCGCATATGACGACGCGAGCAGCTCAATAACGCTGCCAGTTCGTTGAGCGTTGTGTCCTGCGATTTACCCTCGCAGCATTGCCACAGGCGGATGAA  >  NC_000913/76781‑77272

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑