Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	76,364	G→A	A312A (GCC→GCT)	sgrR ←	DNA‑binding transcriptional dual regulator SgrR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	76,364	0	G	A	96.8%	297.8 / NA	95	A312A (GCC→GCT)	sgrR	DNA‑binding transcriptional dual regulator SgrR
Reads supporting (aligned to +/- strand):  ref base G (1/0);  major base A (48/43);  minor base C (1/2);  total (50/45)
Fisher's exact test for biased strand distribution p-value = 6.05e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

GCTCTTTATGGTGCGGGCATGGTGCCAACGGGGGAGCAGTC‑CATAAGCCGGGAACCACAGTTGCTGGTACTGTTCCTCAGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAACAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCGCCCTGTGGACCTTTTAGCATCAGCCCTCCGGCTGG‑CTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGATTGGTGCTGTTGCGAATCACCGCATACGGACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATTCGCGCGGCAGGATCATCGCCGGAACTTGCCCCAGCAGTAACGGTAACCAGCGGTCCGGTTGCGTGAGATGG  >  NC_000913/76115‑76610                                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                        cctctTTATGGTGCGGGCATGGTGCCAACGGGGGAGCA‑TCTCATAAGCCGGGACTCACAGTTGCTGGTAATGTTCCTCAGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGCCTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAACAGTAAATAGTAGCAACCTTCCTCCAGGCGCCTTTCAATCTCTTTTTCCTCGCCCTGTGGACCTTTTAGCATCAGCCCTCCgt                                                                                                                                                                                                                                                        <  2:181596/250‑2 (MQ=255)             gCGGGCATGGTGCCAACGGGGGAGCAGTC‑CATAAGCCGGGACCCACAGTTGCTGGTACTGTTCCTCAGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGG‑Ctcgtcg                                                                                                                                                                                                                                            <  2:702891/250‑1 (MQ=255)             gCGGGCATGGTGCCAACGGGGGAGCAGGC‑CATAAGCCGGGAACCACAGTTGCTGGTACTGTTCCTCAGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGG‑Ctcgtcg                                                                                                                                                                                                                                            <  2:477606/250‑1 (MQ=255)               gggCATGGTGCCAACGGGGGAGCAGTA‑CATAAGCCGGGAACCACAGTTGCTGGTACTGTTCCTCAGCGAAAGAGACCAGATTAGATGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTGCTGATTCGCCCCGCGATGGGGGCGGCTGTCGAATAGTAAATAGGAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTACAAACCCTGTGGACCTTTTCGCATCAAACCGCCAGCTGG‑Ctcgtcagc                                                                                                                                                                                                                                          >  2:862015/1‑247 (MQ=255)                  cATGGTGCCAACGGGGGAGCAGTC‑CATAAGCCGGGAACCACAGTTGCTGGTACTGTTCCTCAGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGG‑CTCGTCGGCAAtt                                                                                                                                                                                                                                      <  1:443869/251‑1 (MQ=255)                     ggagCCAACGGGGGAGCAGTC‑CATAAGCCGGGAACCTCAGTTGCTGGTACTGTTCCTCAGCGAAATAGACCAGATTAGTTGGAGAAAGCACAGAGCGTACCCAGTCCCTGACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGGCCTTTTAGCATCAGACCTCCAGCTGT‑CTCGTCGGCAATTTcc                                                                                                                                                                                                                                   <  2:810841/248‑1 (MQ=255)                     ggTGCCAACGGGGGAGCAGTC‑CATAAGCCGGGAACCACAGTTGCTGGTACTGTTCCTCAGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGG‑CTCGTCGGCAATTTc                                                                                                                                                                                                                                    <  1:726386/250‑1 (MQ=255)                     ggTGCCAACGGGGGAGCAGTA‑CATACGCCGGGAACCACAGCTGCTGGTACTGTTCCTCAGCGAAATTGACCAGATTAGTTGGCGAAAGCACAAAGCTTACCCAGTCCCTGACTTGCTGATTCGCCCCGCGATGGGGGCGGCTGTAGAATAGAAAATAGTAGCCACCTTCCTCCAGGCGGCTTTCACTCCCTTTTTCCTCACCCTGGGGTCATTTTAGCATCAGACCTACAGCTGGACT‑GTCGGCCATTTcc                                                                                                                                                                                                                                   >  2:951395/1‑251 (MQ=255)                       tGCCAACGGGGGAGCAGTC‑CATAAGCCGGGAACCACAGTTGCTGGTACTGTTCCTCAGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGG‑CTCGTCGGCAATTTCCgg                                                                                                                                                                                                                                 >  1:340421/1‑251 (MQ=255)                         ccAACGGGGGAGCAGTC‑CATAATCCGAGAACCACAGTTGCTGGTACTGTTCCTCCGCGACATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGACTAGTAAATAGTAGCAACCTTCCACCAGGCGGCTTTCAATTTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGCCATACcgctgg‑ctagtagacaattaccagc                                                                                                                                                                                                                                >  2:684296/1‑233 (MQ=255)                         ccAACGGGGGAGCAGTC‑CATAAGCCGGGAACCACAGTTGCTGGTACTGTTCCTCAGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGG‑GTCGTCGGCCATTTCCGGc                                                                                                                                                                                                                                >  2:839557/1‑250 (MQ=255)                         ccAACGGGGGAGCAGTC‑CATAAGCCGGGAACCACAGTTGCTGGTACTGTTCCTCAGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGG‑CTCGTCGGCAATTTCCGGCa                                                                                                                                                                                                                               <  1:944756/251‑1 (MQ=255)                             cGGGGGAGTAGTC‑ACTAAGCAGGGAACCACAGTTGCTGGTACTGTTCCTCAGCCAAATAGACCAGATTAGTTGGAGAAAGCACATAGTTTCACCAGTACATGACTTGCTTATTCGCCCCGCGATGGGTTCGGCTGTAGAACAGTAAATCGTAGCACCCTTTTTCCAGGCGGCTTTTAATCTCTTTTTCCTCTCTCTGTCGACTTTTTAGCATCAGTCAACCCTCTGG‑CTCGTCGGCAATTTCCGGCAGAAc                                                                                                                                                                                                                           <  1:687633/251‑1 (MQ=255)                              gggggATCAGTC‑CATCAGCCGGGAACCACAGTGGCTGGTCCTGTTTCTCAGCGAAATAGACCAGATTAGTGGGAGAAAGCACATAGCTTACCCAGTCCCGCACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAATAGCCAACAGTAGCAACCTTCCTCCACGTGTCTTTCAATCTCTGTTTCCTCACCCTGTCTACCTTTTAGCATCAGACCTCCAGCTGG‑CTCGACGGCAATTTCCGGCAGatcc                                                                                                                                                                                                                          <  2:960157/251‑4 (MQ=255)                               ggggAGCAGTC‑CATAAGCCGGGCACCACAGTTGCTGGTACTGTTCCTCAGCGCAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTACATAGTAGCAACCTTCCTACAGGCGGCTTTCACTCTCTTTTTTCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGG‑ATCGTCTGCAATTTCCGGCAGAAccc                                                                                                                                                                                                                         >  2:75635/1‑251 (MQ=255)                                              aaGCCGGGAACCACAGTTGCTGGTACTGTTCCTCAGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGG‑CTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTtcg                                                                                                                                                                                                           >  1:15320/1‑251 (MQ=255)                                                 cctggAACCACAGGTGCTGGTACTGTTCCTCAGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACACAGTCCCTGACTTGCTGATTCGCCCCGCGATGGGGGCGGCTGTCGAATAGGCAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGCTCTGTTAGCCTCAGACCTCCAGCTTG‑CTCGTGGGCAATTTCCGGCAGAACCCAAACGATACCTtggtcg                                                                                                                                                                                                        >  2:757874/4‑251 (MQ=255)                                                 ccGGGAACCACAGTTGCTGGTACTGTTCCTCAGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGG‑CTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTtcgtcg                                                                                                                                                                                                        <  1:665917/251‑1 (MQ=255)                                                 ccGGGAACCACAGTTGCTGGTACTGTTCCTCAGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAAACCTCCAGCTGG‑CTCGTCGGCCATTTCCGGCAGACCCCAGACTTTAACTtcgtcg                                                                                                                                                                                                        >  2:698061/1‑251 (MQ=255)                                                      aaCCACAGTTGCTGGTACTGTTCCTCAGCGAAATAGACCAGATTAGTGGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTTCTGATTCGCCCCGCGATGGGTGCTGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGG‑CTCGTCGGCAATTTCCTGCAGAACCCAGACGTTAACTTCGTCGATTaa                                                                                                                                                                                                   <  2:500359/251‑1 (MQ=255)                                                            aGTTGCGGGTACTGTTCCTCAGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGG‑CTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCg                                                                                                                                                                                             <  2:95261/251‑1 (MQ=255)                                                                gctgtttcTGTCCCTCAGCTAAATAGGACAAATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTGCAGACTCGCCCCGCGATGGGTGCGGCCTTCGAAGAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTGTTAGCATCAGCCCTGCAGCTGG‑CTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTCCGTCGATTAATGCCCGGTaa                                                                                                                                                                                         <  2:25422/244‑1 (MQ=255)                                                                            ccTCAGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGG‑CTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGCTTAATGCCCGGTAACCGAAGAAGTc                                                                                                                                                                              >  1:736908/1‑250 (MQ=255)                                                                            ccTCAGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGG‑CTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCa                                                                                                                                                                             <  1:418129/251‑1 (MQ=255)                                                                               cagcacaaTAGACCAGATTAGTTGGAGAAAGCACATCGCTTAACCAGTCCCTGACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGT‑CTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACATCGGCGATTAATGCCCGGTATCCGAAGAAGTCATc                                                                                                                                                                           <  2:528366/244‑1 (MQ=255)                                                                               cAGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGG‑CTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCg                                                                                                                                                                          <  1:648147/251‑1 (MQ=255)                                                                                   gAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGG‑CTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATg                                                                                                                                                                      <  1:74327/251‑1 (MQ=255)                                                                                   aaaaTAGACCAGATTAGTTGGAGAAAGCACATGGCTTACCCAGTCCATGACTTGCTGATTCGCCCAGCGATGGGCGCGGCCGTCGACTCGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTACATCTCTTTTTCCTCAACCTGTGGACCTGTTAGCCTCAGACCTCAAGCTGG‑CTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCCTCGATTAATGCCCGGTCAACGAAGAAGCCATCGAATg                                                                                                                                                                      >  2:861105/2‑251 (MQ=255)                                                                                    aaaTAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGG‑CTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGc                                                                                                                                                                     <  1:112747/251‑1 (MQ=255)                                                                                     aaTAGACCAGATTAGTTGGAGAAAGCACATAGCTCACCCAGTCCCTGACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGG‑CTCGTCGGCAATTTCCGGCAGAACCCAGACGTTCACTTCGTCGATTAATGCCCGGTAACCGAAGAAGACATCGAATGcc                                                                                                                                                                    >  2:231682/1‑251 (MQ=255)                                                                                        aGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGG‑CTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGa                                                                                                                                                                 <  1:218886/251‑1 (MQ=255)                                                                                         gACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGG‑CTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGa                                                                                                                                                                 >  1:773984/1‑250 (MQ=255)                                                                                          aCCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGG‑CTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAAt                                                                                                                                                               <  1:188539/251‑1 (MQ=255)                                                                                          aCCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTGCTGATTCGCCCCGCGATGGGTGAGGCTGTCGAATCGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGG‑CTCGTCGGCAATTTCCGGCCGAACCCAGCCGTTAACTTCGTCGATTAATGCCCGGTAACCGTAGAAGTCATCGAATGCCTGAAt                                                                                                                                                               >  2:789677/1‑251 (MQ=255)                                                                                           ccAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTTCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGG‑CTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAAtt                                                                                                                                                              <  1:795833/251‑1 (MQ=255)                                                                                            cAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGG‑CTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAAttt                                                                                                                                                             <  1:840406/251‑1 (MQ=255)                                                                                               aTTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAAACTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCCTCAGCCCTCCAGCTGG‑CTCGTCGGCAATTTCAGGCAGAACCCAGACGTTAACCTCGTCGATTAATGCCCGGTTACCGAAGAAGTCATCGATTGCCTGAATTTTc                                                                                                                                                           >  2:60116/1‑250 (MQ=255)                                                                                                         gaAAGCACATAGCTTACCCAGTCCCTGACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGG‑CTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGATTGGt                                                                                                                                                >  1:954020/1‑251 (MQ=255)                                                                                                           aaGCACATAGCTTACCCAGTCCCTGACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGG‑CTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGATTGGTGc                                                                                                                                              >  1:594249/1‑251 (MQ=255)                                                                                                             gCACATAGCTTACCCAGTCCCTGACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGG‑CTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGATTGGTGCTg                                                                                                                                            <  1:341364/251‑1 (MQ=255)                                                                                                              cacaTAGCTTACCCAGTCCCTGACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGG‑CTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGATTGGTGCTGt                                                                                                                                           >  2:18485/1‑251 (MQ=255)                                                                                                              cacaTAGCTTACCCAGTCCCTGACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGG‑CTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCACGGTAACCGAAGAAGTCATCGAATGCCTGCATTTTCAGTTGCTTGGTGCTGt                                                                                                                                           >  1:298669/1‑251 (MQ=255)                                                                                                                caTAGCTTACCCAGTCCCTGACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGACTAGTACATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGG‑CTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGATTGGTGCTGTTg                                                                                                                                         >  1:119978/1‑251 (MQ=255)                                                                                                                    gCTTACCCAGTCCCTGACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGG‑CTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGATTGGTGCTGTTGCgg                                                                                                                                      >  1:249267/1‑249 (MQ=255)                                                                                                                         ccaaGTCCCTGACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCTAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGG‑CTCGTCGGAAATTTCCGGCAGAACCCAGACGTTAACTTCGGCGATTAATGCCCGGGAACCGAAGAAGACATCGAAAGCCTGAATTTTTAGTTGCTTGGTGCTGTTggcggtaccc                                                                                                                                >  2:808018/4‑242 (MQ=255)                                                                                                                          ccAGTCCCTGACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGG‑CTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGATTGGTGCTGTTGCGGATCACCg                                                                                                                               <  2:317713/251‑1 (MQ=255)                                                                                                                           cAGTCCCTGACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAATCGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGG‑CTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTCACCGCCGAAGTCATCGAATGCCTGCATTTTCCGTTGAATGGTGCTGTTGCGGTTCACCGc                                                                                                                              >  2:226692/1‑251 (MQ=255)                                                                                                                                 cTGACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCACTCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGG‑CTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGCATTTTCAGTTGATTGGTGCTGTTTCGGATCACCGCATACgg                                                                                                                        >  2:976805/1‑251 (MQ=255)                                                                                                                                 cTGACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGG‑CTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCCGTTGATTGGTGCTGTTGCGGTTCACCGCATACgg                                                                                                                        >  2:515837/1‑251 (MQ=255)                                                                                                                                       ttcTGATTCTCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGGAGCAATCTGCCTCCAGGCGGCTTTCCATCGCTGTTTCCTCCCCCTGTGTGCCTGTTAGAATCTGACCTCCAGCTGG‑CTCGTCGGCAATTTCCGGCATAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGATTGGTGCTGTTGCGGATCACCGCATTCGGACCgg                                                                                                                   <  2:558750/248‑1 (MQ=255)                                                                                                                                        ggtGATTCGCCCCGCGATGGGGGCGGCTGTCGAATAGTAAAAAGTAGCAACTTCCCCCCCGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGG‑CTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTGCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGATTGGTGCTGTTGCGGATCACCGCATACGGACCGGTg                                                                                                                 <  2:527048/249‑1 (MQ=255)                                                                                                                                        gCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGG‑CTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGATTGGTGCTGTTGCGGATCACCGCATACGGACCGGTg                                                                                                                 <  1:445096/251‑1 (MQ=255)                                                                                                                                         cTGATTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTTTGGACCTTTTAGCATCAGACCTCCAGCTGG‑CTCGTCGGCAATTTCCGGCAGAACCCAGCCGTTAACTTCGTCGATTACTGCCCGGTAACCGACGAAGTCATCGAATGCCTGAATTTTCAGTTGATTGTTGCTGTTGCGGATCACCGCCTACGGCCCGGttc                                                                                                                >  2:783418/1‑249 (MQ=255)                                                                                                                                           gATTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGG‑CTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGATTGGTGCTGTTGCGGATCACCGCATACGGACCGGTGCCg                                                                                                              <  1:698775/251‑1 (MQ=255)                                                                                                                                               cGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGG‑CTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGATTGGTGCTGTTGCGGATCACCGCATACGGACCGGTGCCgatgg                                                                                                          >  2:230976/1‑251 (MQ=255)                                                                                                                                                  cccGCGATGGGTGCGGCTGTCGAATAGTCAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGG‑CTCGTCGGCAATTTCCGGCAGAACCCCGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGACTTTTCAGTTGATTGGTGCTGTTTCGGATCACCGCCTACGGACCGGTGCCgttgggtt                                                                                                       >  2:74667/1‑249 (MQ=255)                                                                                                                                                   ccGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGG‑CTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGATTGGTGCTGTTGCGGATCACCGCATACGGACCGGTGCCgatgggatg                                                                                                      <  1:246559/251‑1 (MQ=255)                                                                                                                                                       gATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGG‑CTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGATTGGTGCTGTTGCGGATCACCGCATACGGACCGGTGCCGATGGGAtggctg                                                                                                  <  1:630738/251‑1 (MQ=255)                                                                                                                                                        aTGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGG‑CTCGTCGGCAATTTCCGGCCGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCCGTTGATTGGTGCTGTTGCGGATCACCGCATACGGACCGGTGCCGATGGGGtggatgg                                                                                                 >  2:807518/1‑251 (MQ=255)                                                                                                                                                          gggtgtgggtgtCGAATAGTAAATATGAGCATCCTTCCTCCAGGCGGCTTTCAACATCTTTCTCCTCACCCTGTGGACCTTTTAGCATCAGACATCCAGCGGG‑CACGTCGGCACTTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAAGGCACGGTAACCGAAGAAGGCATCGAATGCCTTAATTTTCAGGTGATTGGTTCTGTTGCGGATCTCCGCATACGTACCGGTGCCGATTGGAttgctggta                                                                                               <  2:561197/251‑3 (MQ=255)                                                                                                                                                               cGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCTCCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGG‑CTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGATTGGTGCTGTTGCGGATCACCGCATACGGACCGGTGCCGATGGGATGGCTGGCAAAGtt                                                                                          <  1:453489/251‑1 (MQ=255)                                                                                                                                                                 gCTGTCGAATAGTAAATATTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACATTTTAGCATCAGACCTCCAGCTGG‑CTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATAGAATGCCTGAATTTTCAGTTGATTGGTGCTGTTGCGGATCACCGCATACGGACCGATGACGGTGGGAGGGCTGGCGAAGATAc                                                                                        >  2:532901/1‑251 (MQ=255)                                                                                                                                                                    gttgaataGTAAATAGTATCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTTGCCCTTTTAGCATCAGACCTCCAGCTGG‑CTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGATTGGTGCTGTTGCGGATCACCGCATACGGACCGGTGCCGATGGGATGGCTGGCAAAGTTACt                                                                                       <  2:900443/242‑1 (MQ=255)                                                                                                                                                                      cGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGG‑CTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCAGCGAATGCCTGAATTTTCAGTTGATTGGTGCTGTTGCGGATCACCGCATACGGACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAgg                                                                                   <  1:625985/251‑1 (MQ=255)                                                                                                                                                                           aGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGG‑CTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGATTGGTGCTGTTGCGGATCACCGCATACGGACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTc                                                                              <  2:761868/251‑1 (MQ=255)                                                                                                                                                                                 tagtagCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCagct                                                                                                                                                                                                                                                      <  2:253872/78‑1 (MQ=255)                                                                                                                                                                                 tagtagCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCagct                                                                                                                                                                                                                                                      >  1:253872/1‑78 (MQ=255)                                                                                                                                                                                  agtagCAACCTTCCTCCCGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGG‑CTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGATTGGTGCTGTTGCGGATCACCGCATACGGACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATTcg                                                                       >  1:425243/1‑251 (MQ=255)                                                                                                                                                                                  agtagCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTCCCTCACCCTGTCGACCTTTTAGCACCAGACCTCCCGCTGG‑CTCGTCGGCACTTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGACGAAGTCATCGACTGCCTGACTTTTCAGTTGCTTGGCGCTGTTGCGGCTCACCGCATCCGGACCGGCGCCGATGGGCTGGCTGGCCAAGTTACTGAGGGTTTCCCCTTcg                                                                       >  2:901344/1‑251 (MQ=255)                                                                                                                                                                                     agCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGG‑CTCGTCGGCAATTTCCGGCAGAACCCAGACGTTCACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGATTGGTGCTGTTGCGGATCACCGCATACGGACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATTcgcgc                                                                    >  2:210819/1‑251 (MQ=255)                                                                                                                                                                                      gCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGG‑CTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGATTGGTGCTGTTGCGGATCACCGCATACGGACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATTcgcgcg                                                                   <  1:587120/251‑1 (MQ=255)                                                                                                                                                                                      gCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGG‑CTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAAATTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGATTGGTGCTGTTGCGGATCACAGCATACGGACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATTcgcgcg                                                                   >  1:676055/1‑251 (MQ=255)                                                                                                                                                                                       cAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGG‑CTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTACTGCACGGTAACCGAAGAAGTCATCGACTGCCTGAATTTTCAGTTGATTGGTGCTGTTGCGGATCACCGCATACGGACCGGTGCCGATGGGTTGCCTGGCAAAGATCCTTAGGGTTTCCCATTCGCGCgg                                                                  >  2:858692/1‑251 (MQ=255)                                                                                                                                                                                       cAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGG‑CTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGATTGGTGCTGTTGCGGATCACCGCATACGGACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATTCGCGCgg                                                                  <  1:317755/251‑1 (MQ=255)                                                                                                                                                                                       cAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGG‑CTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGATTGGTGCTGTTGCGGATCACCGCATACGGACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATTCGCGCgg                                                                  >  2:866420/1‑251 (MQ=255)                                                                                                                                                                                        aaCCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGG‑CTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGATTGGTGCTGTTGCGGATCACCGCATACGGACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATTCGCGCGGc                                                                 >  1:925080/1‑251 (MQ=255)                                                                                                                                                                                         aCCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGG‑CTCGTCGGCAATTTCCGGCCGAACCCAGACGTTACCTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGATTGGTGCTGTTGCGGATCACCGCATACGGACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATTCGCCCGGc                                                                 >  2:578355/1‑250 (MQ=255)                                                                                                                                                                                         aCCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGG‑CTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGATTGGTGCTGTTGCGGATCACCGCATACGGACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATTCGCGCGGc                                                                 >  1:1618/1‑250 (MQ=255)                                                                                                                                                                                             tcctccAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTACAGCTGG‑CTCGTCGGCACTTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCTGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGATTGGTGCTGTTGCGGATCACCGCATACGGACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATTCGCGCGGCAGGat                                                            >  1:587279/1‑251 (MQ=255)                                                                                                                                                                                              cctccAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGG‑CTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGATTGGTGCTGTTGCGGATCACCGCATACGGACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATTCGCGCGGCAGGatc                                                           >  1:790098/1‑251 (MQ=255)                                                                                                                                                                                                    ggcggcTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGG‑CTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGATTGGTGCTGTTGCGGATCACCGCATACGGACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATTCGCGCGGCAGGATCATCGcc                                                     <  1:224147/251‑1 (MQ=255)                                                                                                                                                                                                     gcggcgTTCAATCCCTTTTTCCGCTCCCTGTGGACCGTTTAGCATCAGACCTCCAGCTGG‑CTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAAATGCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGATTGGTGCTGTTGCGGATCACCGCATACTGACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATTCGCGCGTCAGGATCATCGCCg                                                    <  2:457925/251‑1 (MQ=255)                                                                                                                                                                                                         cTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGG‑CTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGATTGGTGCTGTTGCGGATCACCGCATACGGACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATTCGCGCGGCAGGCTCATCGCCgggac                                                >  2:976632/1‑248 (MQ=255)                                                                                                                                                                                                         cTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAACTGG‑CTCGTCGGCAATTTCAGGCAGACCCCAGACGTTAACTTCGTCGATTAATCCCCGGTAACCGAAGAAGACATAGAATGACTGAATTTTCAGTTGATTGGTGCTGTTGCGGCTCACCGCATACGGACAGGTGACGGTGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATTCGCGCGGCAGGCACATCGCCGGAAc                                                >  1:126843/1‑251 (MQ=255)                                                                                                                                                                                                           ttCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGG‑CTCGTCGCCAATTTCCGGCAGAACCCAGACGTTAACTTCGACGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGATTGGTGCTGTTGCGGATCACCGCATACGGACCGGAGCCGATGGGATGGCTGGCAAAGTTCCTGAGGGTTTCCCATTCGCGCGGCAGGAGCATCGCCGGAACtt                                              >  2:803559/1‑251 (MQ=255)                                                                                                                                                                                                            tCAATCTCTTTTTCCTCCCCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGG‑CTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGATTGGTGCTGTTGCGGATCACCGCATACGGACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATTCGCGCGGCAGGATCATCGCCGGAACtt                                              <  2:773984/250‑1 (MQ=255)                                                                                                                                                                                                                  tctTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGG‑CTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGATTGGTGCTGTTGCGGATCACCGCATACGGACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATTCGCGCGGCAGGATCATCGCCGGAACTTGcccc                                         >  1:347170/1‑249 (MQ=255)                                                                                                                                                                                                                     ttttCCTCACCCTTGGGACCTTTTAGCTTCAGACCTCCAGCTGG‑CTTGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGGCATCGAATGCCTGAATTTTCAGTTGATTGGTGCTGTTGCGGATCACCGCATACGGTCCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCAGTCGCGCGGCAGGATCATCGCCGGAACTTGCCCcag                                       <  2:765427/248‑1 (MQ=255)                                                                                                                                                                                                                       ttCCTCACCCTGTGGACCTGTTAGCATCAGACCTCCAGCTGG‑CTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGATTGGTGCTGTTGCGGATCACCGCATACGGACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATTCGCGCGGCAGGATCATCGCCGGAACTTGCCCcagcaat                                   <  1:473608/250‑3 (MQ=255)                                                                                                                                                                                                                             acccTGTGGACCTTTTAGCATCAGACCTCCAGCTGG‑CTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGATTGGTGCTGTTGCGGATCACCGCATCCGGACCGGAGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATTCGCGCGGCAGGATCATCGCCGGATCTAGCCCCCGCAATAACGGt                             >  1:521099/2‑250 (MQ=255)                                                                                                                                                                                                                              cccTGTGGACCTTTTAGCATCAGACCTCCAGCTGG‑CTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGATTGGTGCTGTTGCGGATCACCGCATACGGACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATTCGCGCGGCAGGATCATCGCCGGAACTTGCCCCAGCAATAACGGTa                            >  1:732974/1‑250 (MQ=255)                                                                                                                                                                                                                                cTGTGGACCTTTTAGCATCAGACCTCCAGCTGG‑CTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGATTGGTGCTGTTGCGGATCACCGCATACGGACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATTCGCGCGGCAGGATCATCGCCGGAACTTGCCCCAGCAATAACGGTAAcc                         >  1:79765/1‑251 (MQ=255)                                                                                                                                                                                                                                  gtgGACCTTTTAGCATCAGACCTCCAGCTGG‑CTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGATTGGTGCTGTTGCGGATCACCGCATACGGACCGGTGCCGATGGGATGGATGGCAAAGTTACTGAGGGTTTCCCATTCGCGCGGCAGGATCATCGCCGGAACTTGCCCCAGCAATAACGGTAACCa                        <  1:137584/250‑1 (MQ=255)                                                                                                                                                                                                                                  gtgGACCTTTTAGCACCATCCCTCACACTGG‑CTTGTCTGCAATTTTCTGCAGAACCCTGCCGTTCACTTTTTCGATTAATGCACGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGATTGGTGCTGTTTCTGATCACCGCATACGGACCGTTGCCGATTGGATGGCTGGCAAAGTTACTGAGTGTTTCCCATTCGCGTGGCATGATCATCTCCGGAACTTGccctagatataccggtaatc                         <  2:143099/249‑18 (MQ=255)                                                                                                                                                                                                                                      cccTTTTAGCATCAGACCTCCAGCTGG‑CTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGATTGGTGCTGTTGCGGATCACCGCATACGGACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATTCGCGCGGCAGGATCATCGCCGGAACTTGCCCCAGCAATAACGGTAACCAGCGGt                   <  1:231682/250‑1 (MQ=255)                                                                                                                                                                                                                                               cATCAGACCTCCAGCTGG‑CTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGATTGGTGCTGTTGCGGATCACCGCATACGGACCGGTGCCGATGGGATGGCTGGCAACGTTACTGAGGGTTTCCCATTCGCGCGGCAGGATCATCGCCGGAACTTTCCCCAGCAATAACGTAAACCAGCGGACCGGTTGCg          >  2:864442/1‑251 (MQ=255)                                                                                                                                                                                                                                                aTCAGACCTCCAGCTGG‑CTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGATTGGTGCTGTTGCGGATCACCGCATACGGACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATTCGCGCGGCAGGATCATCGCCGGAACTTGCCCCAGCAATAACGGTAACCAGCGGTCCGGTTGCGt         >  1:223019/1‑251 (MQ=255)                                                                                                                                                                                                                                                  cagaccTCCAGCTGG‑CTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGATTGGTGCTGTTGCGGATCACCGCATACGGACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATTCGCGCGGCAGGATCATCGCCGGAACTTGCCCCAGCAATAACGGTAACCAGCGGTCCGGTTGCGTg        <  1:803310/246‑1 (MQ=255)                                                                                                                                                                                                                                                       cTCCGGCTGG‑CTCGTCGGCAATTTCCGACAGAACCCAGACGTTAACTTCGTCGCTTAATGCCCGGTAACCGAAGAAGTCAACGAATGCCTGAATTTTCAGTTGATTAGTGCTGTTGCGAATCACCGCATACGGACCGGTGCCGATGGGATGGCTGCCAAGGTTACTGAGGGTTTACCATGCGAGCGGCAGGATCATCGCCGGCAATTGCGCCAGGAGTCACGGTTACCAGCGGTGCGGGTGCGGGAGTTgg  >  2:123774/1‑251 (MQ=255)                                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                        GCTCTTTATGGTGCGGGCATGGTGCCAACGGGGGAGCAGTC‑CATAAGCCGGGAACCACAGTTGCTGGTACTGTTCCTCAGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAACAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCGCCCTGTGGACCTTTTAGCATCAGCCCTCCGGCTGG‑CTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGATTGGTGCTGTTGCGAATCACCGCATACGGACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATTCGCGCGGCAGGATCATCGCCGGAACTTGCCCCAGCAGTAACGGTAACCAGCGGTCCGGTTGCGTGAGATGG  >  NC_000913/76115‑76610

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑