Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	76,358	C→A	G314G (GGG→GGT)	sgrR ←	DNA‑binding transcriptional dual regulator SgrR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	76,358	0	C	A	94.9%	290.0 / ‑5.3	100	G314G (GGG→GGT)	sgrR	DNA‑binding transcriptional dual regulator SgrR
Reads supporting (aligned to +/- strand):  ref base C (2/3);  new base A (49/44);  total (51/49)
Fisher's exact test for biased strand distribution p-value = 6.69e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.73e-01

CGCTCTTTATGGTGCGGGCATGGTGCCAACGGGGGAGCAGTC‑CATAAGCCGGGAACCACAGTTGCTGGTACTGTTCCTCAGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAACAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCGCCCTGTGGACCTTTTAGCATCAGCCCTCCGGCTGG‑CTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGATTGGTGCTGTTGCGAATCACCGCATACGGACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATTCGCGCGGCAGGATCATCGCCGGAACTTGCCCCAGCAGTAACGGTAACCAGCGGTCCGGTTGCGT  >  NC_000913/76114‑76603                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                       ccctctTTATGGTGCGTGCATTGTTCAAACTGGGGAGTAGTC‑AATAAGCCGGGAAACACAGTTGCTGGTACTGTTCCTCAGCGAAAAAGACCAGATTATGTGGATAAAGCACATAGCGTATCCAGTCCCTGACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAATATTAAATAGTAGCAGGCGGCCTGCAGGCTGCCGTCAATCGCTGTTTCCTCCCCCTGTGGACCTCTTAGCAGCAGTCCTcaa                                                                                                                                                                                                                                                  <  2:154379/249‑3 (MQ=255) cGCTCTTTATGGTGCGGGCATGGTGCCAACGGGGGAGCAGTC‑CATAAGCCGGGAACCACAGTTGCTGGTACTGTTCCTCAGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTcca                                                                                                                                                                                                                                                  <  1:668838/251‑2 (MQ=255)  cctctTTATGGTGCGGGCATGGTGCCAACGGGGGAGCA‑TCTCATAAGCCGGGACTCACAGTTGCTGGTAATGTTCCTCAGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGCCTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAACAGTAAATAGTAGCAACCTTCCTCCAGGCGCCTTTCAATCTCTTTTTCCTCGCCCTGTGGACCTTTTAGCATCAGCCCTCCgt                                                                                                                                                                                                                                                 <  2:181596/250‑2 (MQ=255)     ttttATGGTGCGGGCATGGTGCCCACGGGGGAGCAGGC‑CATAAGCCGGGAACCACAGTTGCTGGTACTGTTCCTCAGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGATTTGCTTATTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTccagc                                                                                                                                                                                                                                                <  2:343406/248‑4 (MQ=255)              gCGGGCATGGTGCCAACGGGGGAGCAGTC‑CATAAGCCGGGACCCACAGTTGCTGGTACTGTTCCTCAGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGG‑Ctcgtcg                                                                                                                                                                                                                                     <  2:702891/250‑1 (MQ=255)              gCGGGCATGGTGCCAACGGGGGAGCAGGC‑CATAAGCCGGGAACCACAGTTGCTGGTACTGTTCCTCAGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGG‑Ctcgtcg                                                                                                                                                                                                                                     <  2:477606/250‑1 (MQ=255)                gggCATGGTGCCAACGGGGGAGCAGTA‑CATAAGCCGGGAACCACAGTTGCTGGTACTGTTCCTCAGCGAAAGAGACCAGATTAGATGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTGCTGATTCGCCCCGCGATGGGGGCGGCTGTCGAATAGTAAATAGGAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTACAAACCCTGTGGACCTTTTCGCATCAAACCGCCAGCTGG‑Ctcgtcagc                                                                                                                                                                                                                                   >  2:862015/1‑247 (MQ=255)                   cATGGTGCCAACGGGGGAGCAGTC‑CATAAGCCGGGAACCACAGTTGCTGGTACTGTTCCTCAGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGG‑CTCGTCGGCAAtt                                                                                                                                                                                                                               <  1:443869/251‑1 (MQ=255)                      ggagCCAACGGGGGAGCAGTC‑CATAAGCCGGGAACCTCAGTTGCTGGTACTGTTCCTCAGCGAAATAGACCAGATTAGTTGGAGAAAGCACAGAGCGTACCCAGTCCCTGACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGGCCTTTTAGCATCAGACCTCCAGCTGT‑CTCGTCGGCAATTTcc                                                                                                                                                                                                                            <  2:810841/248‑1 (MQ=255)                      ggTGCCAACGGGGGAGCAGTC‑CATAAGCCGGGAACCACAGTTGCTGGTACTGTTCCTCAGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGG‑CTCGTCGGCAATTTc                                                                                                                                                                                                                             <  1:726386/250‑1 (MQ=255)                      ggTGCCAACGGGGGAGCAGTA‑CATACGCCGGGAACCACAGCTGCTGGTACTGTTCCTCAGCGAAATTGACCAGATTAGTTGGCGAAAGCACAAAGCTTACCCAGTCCCTGACTTGCTGATTCGCCCCGCGATGGGGGCGGCTGTAGAATAGAAAATAGTAGCCACCTTCCTCCAGGCGGCTTTCACTCCCTTTTTCCTCACCCTGGGGTCATTTTAGCATCAGACCTACAGCTGGACT‑GTCGGCCATTTcc                                                                                                                                                                                                                            >  2:951395/1‑251 (MQ=255)                        tGCCAACGGGGGAGCAGTC‑CATAAGCCGGGAACCACAGTTGCTGGTACTGTTCCTCAGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGG‑CTCGTCGGCAATTTCCgg                                                                                                                                                                                                                          >  1:340421/1‑251 (MQ=255)                          ccAACGGGGGAGCAGTC‑CATAATCCGAGAACCACAGTTGCTGGTACTGTTCCTCCGCGACATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGACTAGTAAATAGTAGCAACCTTCCACCAGGCGGCTTTCAATTTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGCCATACcgctgg‑ctagtagacaattaccagc                                                                                                                                                                                                                         >  2:684296/1‑233 (MQ=255)                          ccAACGGGGGAGCAGTC‑CATAAGCCGGGAACCACAGTTGCTGGTACTGTTCCTCAGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGG‑GTCGTCGGCCATTTCCGGc                                                                                                                                                                                                                         >  2:839557/1‑250 (MQ=255)                          ccAACGGGGGAGCAGTC‑CATAAGCCGGGAACCACAGTTGCTGGTACTGTTCCTCAGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGG‑CTCGTCGGCAATTTCCGGCa                                                                                                                                                                                                                        <  1:944756/251‑1 (MQ=255)                              cGGGGGAGTAGTC‑ACTAAGCAGGGAACCACAGTTGCTGGTACTGTTCCTCAGCCAAATAGACCAGATTAGTTGGAGAAAGCACATAGTTTCACCAGTACATGACTTGCTTATTCGCCCCGCGATGGGTTCGGCTGTAGAACAGTAAATCGTAGCACCCTTTTTCCAGGCGGCTTTTAATCTCTTTTTCCTCTCTCTGTCGACTTTTTAGCATCAGTCAACCCTCTGG‑CTCGTCGGCAATTTCCGGCAGAAc                                                                                                                                                                                                                    <  1:687633/251‑1 (MQ=255)                               gggggATCAGTC‑CATCAGCCGGGAACCACAGTGGCTGGTCCTGTTTCTCAGCGAAATAGACCAGATTAGTGGGAGAAAGCACATAGCTTACCCAGTCCCGCACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAATAGCCAACAGTAGCAACCTTCCTCCACGTGTCTTTCAATCTCTGTTTCCTCACCCTGTCTACCTTTTAGCATCAGACCTCCAGCTGG‑CTCGACGGCAATTTCCGGCAGatcc                                                                                                                                                                                                                   <  2:960157/251‑4 (MQ=255)                                ggggAGCAGTC‑CATAAGCCGGGCACCACAGTTGCTGGTACTGTTCCTCAGCGCAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTACATAGTAGCAACCTTCCTACAGGCGGCTTTCACTCTCTTTTTTCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGG‑ATCGTCTGCAATTTCCGGCAGAAccc                                                                                                                                                                                                                  >  2:75635/1‑251 (MQ=255)                                               aaGCCGGGAACCACAGTTGCTGGTACTGTTCCTCAGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGG‑CTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTtcg                                                                                                                                                                                                    >  1:15320/1‑251 (MQ=255)                                                  cctggAACCACAGGTGCTGGTACTGTTCCTCAGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACACAGTCCCTGACTTGCTGATTCGCCCCGCGATGGGGGCGGCTGTCGAATAGGCAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGCTCTGTTAGCCTCAGACCTCCAGCTTG‑CTCGTGGGCAATTTCCGGCAGAACCCAAACGATACCTtggtcg                                                                                                                                                                                                 >  2:757874/4‑251 (MQ=255)                                                  ccGGGAACCACAGTTGCTGGTACTGTTCCTCAGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGG‑CTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTtcgtcg                                                                                                                                                                                                 <  1:665917/251‑1 (MQ=255)                                                  ccGGGAACCACAGTTGCTGGTACTGTTCCTCAGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAAACCTCCAGCTGG‑CTCGTCGGCCATTTCCGGCAGACCCCAGACTTTAACTtcgtcg                                                                                                                                                                                                 >  2:698061/1‑251 (MQ=255)                                                       aaCCACAGTTGCTGGTACTGTTCCTCAGCGAAATAGACCAGATTAGTGGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTTCTGATTCGCCCCGCGATGGGTGCTGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGG‑CTCGTCGGCAATTTCCTGCAGAACCCAGACGTTAACTTCGTCGATTaa                                                                                                                                                                                            <  2:500359/251‑1 (MQ=255)                                                             aGTTGCGGGTACTGTTCCTCAGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGG‑CTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCg                                                                                                                                                                                      <  2:95261/251‑1 (MQ=255)                                                                 gctgtttcTGTCCCTCAGCTAAATAGGACAAATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTGCAGACTCGCCCCGCGATGGGTGCGGCCTTCGAAGAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTGTTAGCATCAGCCCTGCAGCTGG‑CTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTCCGTCGATTAATGCCCGGTaa                                                                                                                                                                                  <  2:25422/244‑1 (MQ=255)                                                                             ccTCAGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGG‑CTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGCTTAATGCCCGGTAACCGAAGAAGTc                                                                                                                                                                       >  1:736908/1‑250 (MQ=255)                                                                             ccTCAGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGG‑CTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCa                                                                                                                                                                      <  1:418129/251‑1 (MQ=255)                                                                                cagcacaaTAGACCAGATTAGTTGGAGAAAGCACATCGCTTAACCAGTCCCTGACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGT‑CTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACATCGGCGATTAATGCCCGGTATCCGAAGAAGTCATc                                                                                                                                                                    <  2:528366/244‑1 (MQ=255)                                                                                cAGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGG‑CTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCg                                                                                                                                                                   <  1:648147/251‑1 (MQ=255)                                                                                    gAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGG‑CTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATg                                                                                                                                                               <  1:74327/251‑1 (MQ=255)                                                                                    aaaaTAGACCAGATTAGTTGGAGAAAGCACATGGCTTACCCAGTCCATGACTTGCTGATTCGCCCAGCGATGGGCGCGGCCGTCGACTCGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTACATCTCTTTTTCCTCAACCTGTGGACCTGTTAGCCTCAGACCTCAAGCTGG‑CTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCCTCGATTAATGCCCGGTCAACGAAGAAGCCATCGAATg                                                                                                                                                               >  2:861105/2‑251 (MQ=255)                                                                                     aaaTAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGG‑CTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGc                                                                                                                                                              <  1:112747/251‑1 (MQ=255)                                                                                      aaTAGACCAGATTAGTTGGAGAAAGCACATAGCTCACCCAGTCCCTGACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGG‑CTCGTCGGCAATTTCCGGCAGAACCCAGACGTTCACTTCGTCGATTAATGCCCGGTAACCGAAGAAGACATCGAATGcc                                                                                                                                                             >  2:231682/1‑251 (MQ=255)                                                                                         aGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGG‑CTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGa                                                                                                                                                          <  1:218886/251‑1 (MQ=255)                                                                                          gACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGG‑CTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGa                                                                                                                                                          >  1:773984/1‑250 (MQ=255)                                                                                           aCCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGG‑CTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAAt                                                                                                                                                        <  1:188539/251‑1 (MQ=255)                                                                                           aCCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTGCTGATTCGCCCCGCGATGGGTGAGGCTGTCGAATCGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGG‑CTCGTCGGCAATTTCCGGCCGAACCCAGCCGTTAACTTCGTCGATTAATGCCCGGTAACCGTAGAAGTCATCGAATGCCTGAAt                                                                                                                                                        >  2:789677/1‑251 (MQ=255)                                                                                            ccAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTTCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGG‑CTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAAtt                                                                                                                                                       <  1:795833/251‑1 (MQ=255)                                                                                             cAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGG‑CTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAAttt                                                                                                                                                      <  1:840406/251‑1 (MQ=255)                                                                                                aTTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAAACTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCCTCAGCCCTCCAGCTGG‑CTCGTCGGCAATTTCAGGCAGAACCCAGACGTTAACCTCGTCGATTAATGCCCGGTTACCGAAGAAGTCATCGATTGCCTGAATTTTc                                                                                                                                                    >  2:60116/1‑250 (MQ=255)                                                                                                          gaAAGCACATAGCTTACCCAGTCCCTGACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGG‑CTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGATTGGt                                                                                                                                         >  1:954020/1‑251 (MQ=255)                                                                                                            aaGCACATAGCTTACCCAGTCCCTGACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGG‑CTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGATTGGTGc                                                                                                                                       >  1:594249/1‑251 (MQ=255)                                                                                                              gCACATAGCTTACCCAGTCCCTGACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGG‑CTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGATTGGTGCTg                                                                                                                                     <  1:341364/251‑1 (MQ=255)                                                                                                               cacaTAGCTTACCCAGTCCCTGACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGG‑CTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGATTGGTGCTGt                                                                                                                                    >  2:18485/1‑251 (MQ=255)                                                                                                               cacaTAGCTTACCCAGTCCCTGACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGG‑CTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCACGGTAACCGAAGAAGTCATCGAATGCCTGCATTTTCAGTTGCTTGGTGCTGt                                                                                                                                    >  1:298669/1‑251 (MQ=255)                                                                                                                 caTAGCTTACCCAGTCCCTGACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGACTAGTACATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGG‑CTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGATTGGTGCTGTTg                                                                                                                                  >  1:119978/1‑251 (MQ=255)                                                                                                                     gCTTACCCAGTCCCTGACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGG‑CTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGATTGGTGCTGTTGCgg                                                                                                                               >  1:249267/1‑249 (MQ=255)                                                                                                                          ccaaGTCCCTGACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCTAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGG‑CTCGTCGGAAATTTCCGGCAGAACCCAGACGTTAACTTCGGCGATTAATGCCCGGGAACCGAAGAAGACATCGAAAGCCTGAATTTTTAGTTGCTTGGTGCTGTTggcggtaccc                                                                                                                         >  2:808018/4‑242 (MQ=255)                                                                                                                           ccAGTCCCTGACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGG‑CTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGATTGGTGCTGTTGCGGATCACCg                                                                                                                        <  2:317713/251‑1 (MQ=255)                                                                                                                            cAGTCCCTGACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAATCGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGG‑CTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTCACCGCCGAAGTCATCGAATGCCTGCATTTTCCGTTGAATGGTGCTGTTGCGGTTCACCGc                                                                                                                       >  2:226692/1‑251 (MQ=255)                                                                                                                                  cTGACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCACTCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGG‑CTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGCATTTTCAGTTGATTGGTGCTGTTTCGGATCACCGCATACgg                                                                                                                 >  2:976805/1‑251 (MQ=255)                                                                                                                                  cTGACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGG‑CTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCCGTTGATTGGTGCTGTTGCGGTTCACCGCATACgg                                                                                                                 >  2:515837/1‑251 (MQ=255)                                                                                                                                        ttcTGATTCTCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGGAGCAATCTGCCTCCAGGCGGCTTTCCATCGCTGTTTCCTCCCCCTGTGTGCCTGTTAGAATCTGACCTCCAGCTGG‑CTCGTCGGCAATTTCCGGCATAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGATTGGTGCTGTTGCGGATCACCGCATTCGGACCgg                                                                                                            <  2:558750/248‑1 (MQ=255)                                                                                                                                         ggtGATTCGCCCCGCGATGGGGGCGGCTGTCGAATAGTAAAAAGTAGCAACTTCCCCCCCGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGG‑CTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTGCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGATTGGTGCTGTTGCGGATCACCGCATACGGACCGGTg                                                                                                          <  2:527048/249‑1 (MQ=255)                                                                                                                                         gCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGG‑CTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGATTGGTGCTGTTGCGGATCACCGCATACGGACCGGTg                                                                                                          <  1:445096/251‑1 (MQ=255)                                                                                                                                          cTGATTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTTTGGACCTTTTAGCATCAGACCTCCAGCTGG‑CTCGTCGGCAATTTCCGGCAGAACCCAGCCGTTAACTTCGTCGATTACTGCCCGGTAACCGACGAAGTCATCGAATGCCTGAATTTTCAGTTGATTGTTGCTGTTGCGGATCACCGCCTACGGCCCGGttc                                                                                                         >  2:783418/1‑249 (MQ=255)                                                                                                                                            gATTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGG‑CTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGATTGGTGCTGTTGCGGATCACCGCATACGGACCGGTGCCg                                                                                                       <  1:698775/251‑1 (MQ=255)                                                                                                                                                cGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGG‑CTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGATTGGTGCTGTTGCGGATCACCGCATACGGACCGGTGCCgatgg                                                                                                   >  2:230976/1‑251 (MQ=255)                                                                                                                                                   cccGCGATGGGTGCGGCTGTCGAATAGTCAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGG‑CTCGTCGGCAATTTCCGGCAGAACCCCGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGACTTTTCAGTTGATTGGTGCTGTTTCGGATCACCGCCTACGGACCGGTGCCgttgggtt                                                                                                >  2:74667/1‑249 (MQ=255)                                                                                                                                                    ccGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGG‑CTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGATTGGTGCTGTTGCGGATCACCGCATACGGACCGGTGCCgatgggatg                                                                                               <  1:246559/251‑1 (MQ=255)                                                                                                                                                        gATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGG‑CTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGATTGGTGCTGTTGCGGATCACCGCATACGGACCGGTGCCGATGGGAtggctg                                                                                           <  1:630738/251‑1 (MQ=255)                                                                                                                                                         aTGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGG‑CTCGTCGGCAATTTCCGGCCGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCCGTTGATTGGTGCTGTTGCGGATCACCGCATACGGACCGGTGCCGATGGGGtggatgg                                                                                          >  2:807518/1‑251 (MQ=255)                                                                                                                                                           gggtgtgggtgtCGAATAGTAAATATGAGCATCCTTCCTCCAGGCGGCTTTCAACATCTTTCTCCTCACCCTGTGGACCTTTTAGCATCAGACATCCAGCGGG‑CACGTCGGCACTTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAAGGCACGGTAACCGAAGAAGGCATCGAATGCCTTAATTTTCAGGTGATTGGTTCTGTTGCGGATCTCCGCATACGTACCGGTGCCGATTGGAttgctggta                                                                                        <  2:561197/251‑3 (MQ=255)                                                                                                                                                                cGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCTCCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGG‑CTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGATTGGTGCTGTTGCGGATCACCGCATACGGACCGGTGCCGATGGGATGGCTGGCAAAGtt                                                                                   <  1:453489/251‑1 (MQ=255)                                                                                                                                                                  gCTGTCGAATAGTAAATATTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACATTTTAGCATCAGACCTCCAGCTGG‑CTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATAGAATGCCTGAATTTTCAGTTGATTGGTGCTGTTGCGGATCACCGCATACGGACCGATGACGGTGGGAGGGCTGGCGAAGATAc                                                                                 >  2:532901/1‑251 (MQ=255)                                                                                                                                                                     gttgaataGTAAATAGTATCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTTGCCCTTTTAGCATCAGACCTCCAGCTGG‑CTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGATTGGTGCTGTTGCGGATCACCGCATACGGACCGGTGCCGATGGGATGGCTGGCAAAGTTACt                                                                                <  2:900443/242‑1 (MQ=255)                                                                                                                                                                       cGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGG‑CTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCAGCGAATGCCTGAATTTTCAGTTGATTGGTGCTGTTGCGGATCACCGCATACGGACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAgg                                                                            <  1:625985/251‑1 (MQ=255)                                                                                                                                                                            aGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGG‑CTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGATTGGTGCTGTTGCGGATCACCGCATACGGACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTc                                                                       <  2:761868/251‑1 (MQ=255)                                                                                                                                                                                  tagtagCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCagct                                                                                                                                                                                                                                               >  1:253872/1‑78 (MQ=255)                                                                                                                                                                                  tagtagCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCagct                                                                                                                                                                                                                                               <  2:253872/78‑1 (MQ=255)                                                                                                                                                                                   agtagCAACCTTCCTCCCGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGG‑CTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGATTGGTGCTGTTGCGGATCACCGCATACGGACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATTcg                                                                >  1:425243/1‑251 (MQ=255)                                                                                                                                                                                   agtagCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTCCCTCACCCTGTCGACCTTTTAGCACCAGACCTCCCGCTGG‑CTCGTCGGCACTTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGACGAAGTCATCGACTGCCTGACTTTTCAGTTGCTTGGCGCTGTTGCGGCTCACCGCATCCGGACCGGCGCCGATGGGCTGGCTGGCCAAGTTACTGAGGGTTTCCCCTTcg                                                                >  2:901344/1‑251 (MQ=255)                                                                                                                                                                                      agCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGG‑CTCGTCGGCAATTTCCGGCAGAACCCAGACGTTCACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGATTGGTGCTGTTGCGGATCACCGCATACGGACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATTcgcgc                                                             >  2:210819/1‑251 (MQ=255)                                                                                                                                                                                       gCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGG‑CTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGATTGGTGCTGTTGCGGATCACCGCATACGGACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATTcgcgcg                                                            <  1:587120/251‑1 (MQ=255)                                                                                                                                                                                       gCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGG‑CTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAAATTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGATTGGTGCTGTTGCGGATCACAGCATACGGACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATTcgcgcg                                                            >  1:676055/1‑251 (MQ=255)                                                                                                                                                                                        cAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGG‑CTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTACTGCACGGTAACCGAAGAAGTCATCGACTGCCTGAATTTTCAGTTGATTGGTGCTGTTGCGGATCACCGCATACGGACCGGTGCCGATGGGTTGCCTGGCAAAGATCCTTAGGGTTTCCCATTCGCGCgg                                                           >  2:858692/1‑251 (MQ=255)                                                                                                                                                                                        cAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGG‑CTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGATTGGTGCTGTTGCGGATCACCGCATACGGACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATTCGCGCgg                                                           >  2:866420/1‑251 (MQ=255)                                                                                                                                                                                        cAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGG‑CTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGATTGGTGCTGTTGCGGATCACCGCATACGGACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATTCGCGCgg                                                           <  1:317755/251‑1 (MQ=255)                                                                                                                                                                                         aaCCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGG‑CTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGATTGGTGCTGTTGCGGATCACCGCATACGGACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATTCGCGCGGc                                                          >  1:925080/1‑251 (MQ=255)                                                                                                                                                                                          aCCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGG‑CTCGTCGGCAATTTCCGGCCGAACCCAGACGTTACCTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGATTGGTGCTGTTGCGGATCACCGCATACGGACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATTCGCCCGGc                                                          >  2:578355/1‑250 (MQ=255)                                                                                                                                                                                          aCCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGG‑CTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGATTGGTGCTGTTGCGGATCACCGCATACGGACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATTCGCGCGGc                                                          >  1:1618/1‑250 (MQ=255)                                                                                                                                                                                              tcctccAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTACAGCTGG‑CTCGTCGGCACTTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCTGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGATTGGTGCTGTTGCGGATCACCGCATACGGACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATTCGCGCGGCAGGat                                                     >  1:587279/1‑251 (MQ=255)                                                                                                                                                                                               cctccAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGG‑CTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGATTGGTGCTGTTGCGGATCACCGCATACGGACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATTCGCGCGGCAGGatc                                                    >  1:790098/1‑251 (MQ=255)                                                                                                                                                                                                     ggcggcTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGG‑CTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGATTGGTGCTGTTGCGGATCACCGCATACGGACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATTCGCGCGGCAGGATCATCGcc                                              <  1:224147/251‑1 (MQ=255)                                                                                                                                                                                                      gcggcgTTCAATCCCTTTTTCCGCTCCCTGTGGACCGTTTAGCATCAGACCTCCAGCTGG‑CTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAAATGCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGATTGGTGCTGTTGCGGATCACCGCATACTGACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATTCGCGCGTCAGGATCATCGCCg                                             <  2:457925/251‑1 (MQ=255)                                                                                                                                                                                                          cTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGG‑CTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGATTGGTGCTGTTGCGGATCACCGCATACGGACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATTCGCGCGGCAGGCTCATCGCCgggac                                         >  2:976632/1‑248 (MQ=255)                                                                                                                                                                                                          cTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAACTGG‑CTCGTCGGCAATTTCAGGCAGACCCCAGACGTTAACTTCGTCGATTAATCCCCGGTAACCGAAGAAGACATAGAATGACTGAATTTTCAGTTGATTGGTGCTGTTGCGGCTCACCGCATACGGACAGGTGACGGTGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATTCGCGCGGCAGGCACATCGCCGGAAc                                         >  1:126843/1‑251 (MQ=255)                                                                                                                                                                                                            ttCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGG‑CTCGTCGCCAATTTCCGGCAGAACCCAGACGTTAACTTCGACGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGATTGGTGCTGTTGCGGATCACCGCATACGGACCGGAGCCGATGGGATGGCTGGCAAAGTTCCTGAGGGTTTCCCATTCGCGCGGCAGGAGCATCGCCGGAACtt                                       >  2:803559/1‑251 (MQ=255)                                                                                                                                                                                                             tCAATCTCTTTTTCCTCCCCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGG‑CTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGATTGGTGCTGTTGCGGATCACCGCATACGGACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATTCGCGCGGCAGGATCATCGCCGGAACtt                                       <  2:773984/250‑1 (MQ=255)                                                                                                                                                                                                                   tctTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGG‑CTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGATTGGTGCTGTTGCGGATCACCGCATACGGACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATTCGCGCGGCAGGATCATCGCCGGAACTTGcccc                                  >  1:347170/1‑249 (MQ=255)                                                                                                                                                                                                                      ttttCCTCACCCTTGGGACCTTTTAGCTTCAGACCTCCAGCTGG‑CTTGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGGCATCGAATGCCTGAATTTTCAGTTGATTGGTGCTGTTGCGGATCACCGCATACGGTCCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCAGTCGCGCGGCAGGATCATCGCCGGAACTTGCCCcag                                <  2:765427/248‑1 (MQ=255)                                                                                                                                                                                                                        ttCCTCACCCTGTGGACCTGTTAGCATCAGACCTCCAGCTGG‑CTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGATTGGTGCTGTTGCGGATCACCGCATACGGACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATTCGCGCGGCAGGATCATCGCCGGAACTTGCCCcagcaat                            <  1:473608/250‑3 (MQ=255)                                                                                                                                                                                                                              acccTGTGGACCTTTTAGCATCAGACCTCCAGCTGG‑CTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGATTGGTGCTGTTGCGGATCACCGCATCCGGACCGGAGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATTCGCGCGGCAGGATCATCGCCGGATCTAGCCCCCGCAATAACGGt                      >  1:521099/2‑250 (MQ=255)                                                                                                                                                                                                                               cccTGTGGACCTTTTAGCATCAGACCTCCAGCTGG‑CTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGATTGGTGCTGTTGCGGATCACCGCATACGGACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATTCGCGCGGCAGGATCATCGCCGGAACTTGCCCCAGCAATAACGGTa                     >  1:732974/1‑250 (MQ=255)                                                                                                                                                                                                                                 cTGTGGACCTTTTAGCATCAGACCTCCAGCTGG‑CTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGATTGGTGCTGTTGCGGATCACCGCATACGGACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATTCGCGCGGCAGGATCATCGCCGGAACTTGCCCCAGCAATAACGGTAAcc                  >  1:79765/1‑251 (MQ=255)                                                                                                                                                                                                                                   gtgGACCTTTTAGCATCAGACCTCCAGCTGG‑CTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGATTGGTGCTGTTGCGGATCACCGCATACGGACCGGTGCCGATGGGATGGATGGCAAAGTTACTGAGGGTTTCCCATTCGCGCGGCAGGATCATCGCCGGAACTTGCCCCAGCAATAACGGTAACCa                 <  1:137584/250‑1 (MQ=255)                                                                                                                                                                                                                                   gtgGACCTTTTAGCACCATCCCTCACACTGG‑CTTGTCTGCAATTTTCTGCAGAACCCTGCCGTTCACTTTTTCGATTAATGCACGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGATTGGTGCTGTTTCTGATCACCGCATACGGACCGTTGCCGATTGGATGGCTGGCAAAGTTACTGAGTGTTTCCCATTCGCGTGGCATGATCATCTCCGGAACTTGccctagatataccggtaatc                  <  2:143099/249‑18 (MQ=255)                                                                                                                                                                                                                                       cccTTTTAGCATCAGACCTCCAGCTGG‑CTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGATTGGTGCTGTTGCGGATCACCGCATACGGACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATTCGCGCGGCAGGATCATCGCCGGAACTTGCCCCAGCAATAACGGTAACCAGCGGt            <  1:231682/250‑1 (MQ=255)                                                                                                                                                                                                                                                cATCAGACCTCCAGCTGG‑CTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGATTGGTGCTGTTGCGGATCACCGCATACGGACCGGTGCCGATGGGATGGCTGGCAACGTTACTGAGGGTTTCCCATTCGCGCGGCAGGATCATCGCCGGAACTTTCCCCAGCAATAACGTAAACCAGCGGACCGGTTGCg   >  2:864442/1‑251 (MQ=255)                                                                                                                                                                                                                                                 aTCAGACCTCCAGCTGG‑CTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGATTGGTGCTGTTGCGGATCACCGCATACGGACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATTCGCGCGGCAGGATCATCGCCGGAACTTGCCCCAGCAATAACGGTAACCAGCGGTCCGGTTGCGt  >  1:223019/1‑251 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                       CGCTCTTTATGGTGCGGGCATGGTGCCAACGGGGGAGCAGTC‑CATAAGCCGGGAACCACAGTTGCTGGTACTGTTCCTCAGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAACAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCGCCCTGTGGACCTTTTAGCATCAGCCCTCCGGCTGG‑CTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGATTGGTGCTGTTGCGAATCACCGCATACGGACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATTCGCGCGGCAGGATCATCGCCGGAACTTGCCCCAGCAGTAACGGTAACCAGCGGTCCGGTTGCGT  >  NC_000913/76114‑76603

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑