Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	65,057	G→T	L242I (CTT→ATT)	polB ←	DNA polymerase II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	65,057	0	G	T	97.9%	303.2 / ‑1.4	95	L242I (CTT→ATT)	polB	DNA polymerase II
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base T (50/43);  total (51/44)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.68e-01

AAACGGCGGTCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAGCTCCTGAGCGACAGTT‑TCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAA‑GACG‑CCGTTTTTAAAGCCGTGCTCGCGCC‑ACTCCAGCTCGCTATTATCGC‑GCCCAAGACGCAGCGGAAGACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGCAACTGCGGGCGGCTGGCGACGTATTCCAGTTCGAAATCAAGCGAGGAGGCGTCGCCATTCTCCGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCG  >  NC_000913/64819‑65300                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                    aaaCGTCGGTCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTT‑TCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAA‑GACG‑CCGTTTTTAAAGCCGTGCTCGCGCC‑ACTCCAGCTCACTGTTATCGC‑GCCCCAGCCGCAGCGGAATACGGTAACGCTc                                                                                                                                                                                                                                         <  2:171737/251‑1 (MQ=255) aaaCGGCGGTCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTT‑TCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAA‑GACG‑CCGTTTTTAAAGCCGTGCTCGCGCC‑ACTCCAGCTCACTGTTATCGC‑GCCCCAGCCGCAGCGGAATACGGTAACGCTc                                                                                                                                                                                                                                         <  1:103569/251‑1 (MQ=255) aaaCGGCGGTCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTT‑TCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAA‑GACG‑CCGTTTTTAAAGCCGTGCTCGCGCC‑ACTCCAGCTCACTGTTATCGC‑GCCCCAGCCGCAGCGGAATACGGTAACGCTc                                                                                                                                                                                                                                         >  1:305255/1‑251 (MQ=255)     ggcggTCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTT‑TCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAA‑GACG‑CCGTTTTTAAAGCCGTGCTCGCGCC‑ACTCCAGCTCACTGTTATCGC‑GCCCCAGCCGCAGCGGAATACGGTAACGATCGGc                                                                                                                                                                                                                                      >  2:798476/1‑250 (MQ=255)      gcggTCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTT‑TCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAA‑GACG‑CCGTTTTTAAAGCCGTGCTCGCGCC‑ACTCCAGCTCACTGTTATCGC‑GCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCAt                                                                                                                                                                                                                                    <  1:930517/251‑1 (MQ=255)        ggTCAATTTCGTCCATTCGATCCCACGGGTTATCTATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTT‑TCCAGCGAGAATGAAGAGAAAGTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAA‑GACG‑CCGTTTTTAAAGCCGTGCTCGCGCC‑ACTCCAGCTCACTGTTATCGC‑GCCCCAGCCGCAGCGGAATACgg                                                                                                                                                                                                                                                 <  2:548157/236‑1 (MQ=255)        ggTCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTT‑TCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAA‑GACG‑CCGTTTTTAAAGCCGTGCTCGCGCC‑ACTCCAGCTCACTGTTATCGC‑GCCCCAGCCGCAGCGGAATACgg                                                                                                                                                                                                                                                 >  1:548157/1‑236 (MQ=255)        agTCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCTCCTAATAACTCCTGAGCGACGGTT‑TCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGACGATAATTAGCCGACCTTTAGCCTGGGCAAAACA‑GACG‑CCGTTTTTAAAGCCGGGCTCGCGCC‑ACACCAACTCACTGTTATCGC‑GCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGt                                                                                                                                                                                                                                  >  2:787095/2‑251 (MQ=255)           cAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTT‑TCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAA‑GACG‑CCGTTTTTAAAGCCGTGCTCGCGCC‑ACTCCAGCTCACTGTTATCGC‑GCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGttt                                                                                                                                                                                                                                <  1:660940/250‑1 (MQ=255)                 cGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTT‑TCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAA‑GACG‑CCGTTTTTAAAGCCGTGCTCGCGCC‑ACTCCAGCTCACTGTTATCGC‑GCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTgcag                                                                                                                                                                                                                          <  1:389720/250‑1 (MQ=255)                    ccATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTT‑TCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAA‑GACG‑CCGTTTTTAAAGCCGTGCTCGCGCC‑ACTCCAGCTCACTGTTATCGC‑GCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCAtt                                                                                                                                                                                                                      >  1:72330/1‑251 (MQ=255)                    ccATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTT‑TCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAA‑GACG‑CCGTTTTTAAAGCCGTGCTCGCGCC‑ACTCCAGCTCACTGTTATCGC‑GCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCAtt                                                                                                                                                                                                                      >  2:297300/1‑251 (MQ=255)                     catccgaccccACGGGTTACCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTT‑TCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGCCTATAATTAGCCGACCTTTAGCCTGGGCAAAAAA‑GACG‑CCGTTTTTAAAGCCGTGCTCGCGCC‑ACTCCAGCTCACTGTTATCGC‑GCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTc                                                                                                                                                                                                                     <  2:682494/243‑1 (MQ=255)                      aTTCGATACCACGGGATATAGGTAGCTTTACCTTCGCCTAATAACTCCTGAGCGACGGTC‑TCCAGCGAGAATGAAGAGCAATTCCAGAACGCGGATTTCAGCGACTCGATACCATCGCTAATTAGCCGACCTTTCGCCTGGGCAAACAA‑GACG‑CCGTTTTTAAAGCCGTGCTCGCGCC‑ACTCCAGCTCACTGTTATCGCCACCCATCA‑GCAGCGGCATACGGTACCGCTCGGAATGtttttccggcatacg                                                                                                                                                                                                                    >  2:410925/1‑241 (MQ=255)                         cgaccccACGGGTTATCGTTAGATGTTCCTGCGCCTAAGAACTCCTGTGCGACGGTT‑TCCAGCGAGAATGAAGAGAAATTCCAGACCGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAA‑GACG‑CCGTTTTTAAAGCCGTGCTCGCGCC‑ACTCCAGCTCACTGTTATCGC‑GCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAg                                                                                                                                                                                                                 <  2:954131/247‑1 (MQ=255)                         cGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTT‑TCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAA‑GACG‑CCGTTTTTAAAGCCGTGCTCGCGCC‑ACTCCAGCTCACTGTTATCGC‑GCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCa                                                                                                                                                                                                                  <  1:840889/250‑1 (MQ=255)                          gATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTT‑TCCAGAGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAA‑GACG‑CCGTTTTTAAAGCCGTGCTCGCGCC‑ACTCCAGCTCACTGTTATCGC‑GCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGc                                                                                                                                                                                                                   >  1:509447/1‑248 (MQ=255)                           aTCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGAC‑GTTCTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATCCCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAA‑GACG‑CCGTTTTTAAAGCCGTGCTCGCGCC‑ACTCCAGCTCACTGTTATCGC‑GTCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGAt                                                                                                                                                                                                               <  1:406753/251‑1 (MQ=255)                             cccACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTT‑TCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAA‑GACG‑CCGTTTTTAAAGCCGTGCTCGCGCC‑ACTCCAGCTCACTGTTATCGC‑GCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGc                                                                                                                                                                                                                   >  1:327960/1‑245 (MQ=255)                               cACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTT‑TCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAA‑GACG‑CCGTTTTTAAAGCCGTGCTCGCGCC‑ACTCCAGCTCACTGTTATCGC‑ACCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTATTGCAGCATTCgacgaaatg                                                                                                                                                                                                            >  2:153279/1‑242 (MQ=255)                                      tATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTT‑TCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAA‑GACG‑CCGTTTTTAAAGCCGTGCTCGCGCC‑ACTCCAGCTCACTGTTATCGC‑GCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTGTGCAGCATTCGCAGATCGAACTGcac                                                                                                                                                                                                     <  1:788722/250‑1 (MQ=255)                                      tATCGATAGATTTTCCTGCGCCGAATAACCCCTGAGCGACGGTT‑TCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAA‑GACG‑CCGTTTTTAAAGCCGTGCTCGCGCC‑ACTCCAGCTCACTGTTATCGC‑GCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGcaa                                                                                                                                                                                                     <  2:228135/250‑2 (MQ=255)                                         cGATAGATTTTCATTCGCCTAATAACTCCTGAGCGACGGTT‑TCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAA‑GACG‑CCGTTTTTAAAGCCGTGCTCGCGCC‑ACTCCAGCTCACTGTTATCGC‑GCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACg                                                                                                                                                                                                 >  1:741151/1‑251 (MQ=255)                                                     cTTCGCCTAATAGCTCCTGAGCGACAGTT‑TCCAGCGAGAATGAAGAGAAATTCTAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAA‑GACG‑CCGTTTTTAAAGCCGTGCTCGCGCC‑ACTCCAGCTCGCTATTATCGC‑GCCCAAGACGCAGCGGAAGACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGt                                                                                                                                                                                                >  1:682542/1‑240 (MQ=255)                                                     cTTCGCCTAATAGCCCGTGAGCGACAGTT‑TCCAGCGAGAATGAAGAGAAATTCTAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGCCCTTTAGCCTGGGCAAAAAA‑GACG‑CCGTTTTTAAAGCCGTGCTCGCGCC‑ACTCCAGCTCGCTATTATCGC‑GCCCAAGACGCAGCGGAAGACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGt                                                                                                                                                                                                <  2:682542/240‑1 (MQ=255)                                                        cGCCTAAAAACTCCTGAGCTACGGTT‑TCCAGCGAGAATGAAGAGAAATTCCAGAACTCGGATTTCAGCGCCCCGATACCGTCGATAATTAGCCGACCTCTAGCCTGGGCAAAAAA‑GACG‑CCGTTTTTAAAGCCGTGCTCGCGCC‑ACTCCAGCTCACTGTTATCGC‑GCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATc                                                                                                                                                                                  <  2:183517/251‑1 (MQ=255)                                                               taacTCCTGAGCGACGGTT‑TCCATCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAACGCCTCGATACCGTCGATACTTAGCCGACCTTTAGCCTGGGCAAAAAA‑GACG‑CCGTTTTTAAAGCCGTGCTCGCGCC‑ACTCCAGCTCACTGTTATCGC‑GCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTCGCAGCATTCGCAGATCGCACTGCACCACTTTCCACCCgcgtatcacatc                                                                                                                                                                             >  2:611922/4‑238 (MQ=255)                                                                 acTCCTGAGCGACGGTT‑TCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAA‑GACG‑CCGTTTTTAAAGCCGTGCTCGCGCC‑ACTCCAGCTCACTGTTATCGC‑GCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAgg                                                                                                                                                                          >  1:316078/2‑250 (MQ=255)                                                                   tCCTGAGCGACGGTT‑TCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAA‑GACG‑CCGTTTTTAAAGCCGTGCTCGCGCC‑ACTCCAGCTCACTGTTATCGC‑GACCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCGTTCGCAGATCGAACTGCACCCCGTTCCAACCGAGGATAACATCAggccc                                                                                                                                                                       >  2:282778/1‑248 (MQ=255)                                                                    ccTGAGCGACGGTT‑TCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAA‑GACG‑CCGTTTTTAAAGCCGTGCTCGCGCC‑ACTCCAGCTCACTGTTATCGC‑GCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCg                                                                                                                                                                      <  1:198777/251‑1 (MQ=255)                                                                     cTGAGCGACGGTT‑TCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAC‑GACG‑CCGTTTTTAAAGCCGTGCTCGCGCC‑ACTCCAGCTCACTGTTATCGC‑GCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCCGGATCGt                                                                                                                                                                     >  2:229483/1‑251 (MQ=255)                                                                       gAGCGACGGTT‑TACAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAA‑GACG‑CCGTTTTTAAAGCCGTGCTCGCGCC‑ACTCCAGCTCACTGTTATCGC‑GCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAg                                                                                                                                                                   <  1:676429/251‑1 (MQ=255)                                                                           gacggTT‑TCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGCTTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAC‑GACG‑CCGTTTTTAAAGCCGTGCTCGCGCC‑ACTCCAGCTCACTGTTATCGC‑GCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGACCTGCACCACGTTCCAACCGATGATCACATCAGGCTCGTAGTTgg                                                                                                                                                               >  2:651521/5‑251 (MQ=255)                                                                                        cGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATCCCGTCGATAATTAGCCGCCCTTTAGCCTGGGCAAAAAA‑GACG‑CCGTTTTTAAAGCCGTGCTCGCGCC‑ACTCCAGCTCACTGTTATCGC‑GCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCGTTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGt                                                                                                                                                   <  1:695633/251‑1 (MQ=255)                                                                                          agaATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAA‑GACG‑CCGTTTTTAAAGCCGTGCTCGCGCC‑ACTCCAGCTCACTGTTATCGC‑GCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTg                                                                                                                                                 <  1:825712/251‑1 (MQ=255)                                                                                           gaATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAA‑GACG‑CCGTTTTTAAAGCCGTGCTCGCGCC‑ACTCCAGCTCCCTGTTATCGC‑GCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCCCGTTCCAACCGATGATCCCATCAGGCTCGTGGTTGGCAAACCAGGCGTTgg                                                                                                                                                >  2:370960/1‑250 (MQ=255)                                                                                                 agagAAATTCCAGAACGAGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAA‑GACG‑CCGTTTTTAAAGCCGTGCTCGCGCC‑ACTCCAGCTCACTGTTATCGC‑GCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGCGttttt                                                                                                                                          >  2:919965/1‑251 (MQ=255)                                                                                                  gagaAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAA‑GACG‑CCGTTTTTAAAGCCGTGCTCGCGCC‑ACTCCAGCTCACTGTTATCGC‑GCCCCAGCCGCAGCGGCATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCCCCAAGTTCCAACCGATGATCACATCAGGTTCGTAGTTGGCAAACCAGGCGTTGAGTTTTTc                                                                                                                                         >  2:935836/1‑251 (MQ=255)                                                                                                  gagaAAGTCCAGACCGCGGATTTCAGCGCCTCGATTCCGCTGATAATTAGCCGACCCTTATCCTGGGCAAAAAA‑GACG‑CCGTTTTTAAAGCCGTGCTCGCGCC‑ACTCCAGCTCACTGTTATCGC‑GCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGAGCGATCTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTc                                                                                                                                         <  2:885295/251‑1 (MQ=255)                                                                                                      aataccAGAACCCTGATTTCAGCGCCTCGATTCCGGAGATAATTATCCGCCCTTTAGCCTGGGCAAAAAA‑GACG‑CCGTGTTTAAAGCCGTGCTCGCGCC‑TCGCCAGCTCACTGTTATCGC‑GCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAgt                                                                                                                                     <  2:361865/247‑2 (MQ=255)                                                                                                       aTTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAA‑GACG‑CCGTTTTTAAAGCCGTGCTCGCGCC‑ACTCCAGCTCACTGTTATCGC‑GCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAgt                                                                                                                                     >  1:246798/1‑249 (MQ=255)                                                                                                         tCCAGAACGCGGATCTCAGCGCCCCGATACCGTCGATAATTAGCCCGCCTTGAGCCTGGGCAAAAAA‑GACT‑CCGATTTTAAAGCCGTGCTCGCGCC‑TCTCCAGCGCACTGTTATCGC‑GCCTCAGCCGCAGCGGAATACGGTAACGCTCGTCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAgtag                                                                                                                                   <  2:789825/250‑4 (MQ=255)                                                                                                                cgcgGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAA‑GACG‑CCGTTTTTAAAGCCGTGCTCGCGCC‑ACTCCAGCTCACTGTTATCGC‑GCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTgcgggc                                                                                                                           <  1:767449/251‑1 (MQ=255)                                                                                                                  cgGATTTCAGCGCCTCGATACCGTCGATAATTAGCCTACCTTTAGCCTGGGCAAAAAA‑GACG‑CCGTTTTTAAAGCCGTGCTCGCGCC‑ACTCCAGCTCACTGTTATCGC‑GCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACAACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTgcgggcgg                                                                                                                         <  1:618221/251‑1 (MQ=255)                                                                                                                  cgGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAA‑GACG‑CCGTTTTTAAAGCCGTGCTCGCGCC‑ACTCCAGCTCACTGTTATCGC‑GCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTgcgggcgg                                                                                                                         >  1:936929/1‑251 (MQ=255)                                                                                                                    gttttCAGCGCCACGATACCGTCGATAATTAGCCGCCCTTTAGCCTGGGCAAAAAA‑GACG‑CCGTTTTTAAAGCCGTGCTCGCGCC‑ACTCCAGCTCACTGTTATCGC‑GCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCt                                                                                                                       <  2:489983/249‑1 (MQ=255)                                                                                                                        tCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAA‑GACG‑CCGTTTTTAAAGCCGTGCTCGCGCC‑ACTCCAGCTCACTGTTATCGC‑GCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCg                                                                                                                   >  2:229987/1‑251 (MQ=255)                                                                                                                          cgcgcCTCGTTACCGTCGATAATTAGCCGACCTTTCGCCTGGGCAAAAAA‑GACC‑CCGTTTTTAAAGCCGTGCTCGCGCC‑ACTCCAGCTCACTGTTATCGC‑GCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGAc                                                                                                                 <  2:313509/250‑1 (MQ=255)                                                                                                                          aGCGCCTCGATACCGACGATATTTAGCCGTCTTTTAGCCTGGGCAAAAAA‑GACG‑CCGTTTTTAAAGTTGTGCTCGCGCC‑ACTCCAGCTCGCTGTTATCGC‑GCTCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGACCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGTCGAc                                                                                                                 <  1:611922/251‑1 (MQ=255)                                                                                                                             gcCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAA‑GACG‑CCGTTTTTAAAGCCGTGCTCGCGCC‑ACTCCAGCTCACTGTTATCGC‑GCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGt                                                                                                               >  1:293319/1‑250 (MQ=255)                                                                                                                                tCGATACAGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAA‑GACG‑CCGTTTTTAAAGCCGTGCTCGCGCC‑ACTCCAGCTCACTGTTATCGC‑GCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCACCCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTc                                                                                                           >  2:908327/1‑251 (MQ=255)                                                                                                                                 cGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAA‑TACG‑CCGTTTTTAAAGCCGTGCTCGCGCC‑ACTCCAGCTCACTGTTATCGC‑GCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTc                                                                                                           <  1:387540/250‑1 (MQ=255)                                                                                                                                 cGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAA‑GACG‑CCGTTTTTAAAGCCGTGCTCGCGCC‑ACTCCAGCTCACTGTTATCGC‑GCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTcc                                                                                                          <  1:798476/251‑1 (MQ=255)                                                                                                                                  gATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAA‑GACG‑CCGTTTTTAAAGCCGTGCTCGCGCC‑ACTCCAGCTCACTGTTATCGC‑GCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCa                                                                                                         <  1:556249/251‑1 (MQ=255)                                                                                                                                       cGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAA‑GACG‑CCGTTTTTAAAGCCGTGCTCGCGCC‑ACTCCAGCTCACTGTTATCGC‑GCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTc                                                                                                     >  1:223311/1‑250 (MQ=255)                                                                                                                                         ccGATAATTAGCCGACCTTTAGCCTGGGCAAAAAA‑GACG‑CCGTTTTTAAAGCCGTGCTCGCGCC‑ACTCCAGCTCACTGTTATCGC‑GCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTcaaa                                                                                                  <  1:587659/250‑4 (MQ=255)                                                                                                                                          cGATAATTAGCCGACCTTTAGCCTGGGAAAAAAA‑GACG‑CCGTTTTTAAGGCCGTGCTCGCGCC‑ACTCCAGCTCCCTGTTATCGC‑GCCACAGCCGCAGCGGAATACGGTAACGCTGGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACAACGTTACAACCGATGCTCACATCAGGGTCGTAGTTGGCAAACCAGGCGATGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGttaaaa                                                                                                  >  2:581017/1‑246 (MQ=255)                                                                                                                                           gATAATTAGCCGACCTTTAGCCTGGGCAAAAAA‑GACG‑CCGTTTTTAAAGCCGTGCTCGCGCC‑ACTCCAGCTCACTGTTATCGC‑GCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAAt                                                                                                >  1:799297/1‑251 (MQ=255)                                                                                                                                               aTTAGCCGACCTTTAGCCTGGGCAAAAAA‑GACG‑CCGTTTTTAAAGCCGTGCTCGCGCC‑ACTCCAGCTCACTGTTATCGC‑GCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATcg                                                                                              >  1:545960/1‑248 (MQ=255)                                                                                                                                                  aGCCGACCTTTAGCCTGGGCAAAAAA‑GCCG‑CCGTTTTTAAAGCCGTGCTCGCGCC‑ACTCCAGCTCACTGTTATCGC‑GCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCgc                                                                                         >  1:330562/1‑250 (MQ=255)                                                                                                                                                    ccGACCTTTGGCCGGGGCAAAAAATGA‑G‑CCGTTTTTAAAGCAGTGCCCGCGCC‑ACGCCAGCTCACTGTTAACGC‑GCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTGCCAACCGATGATCACAGCAGGATCTTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGGAGCTGCGGGCGTCTGGCGACGTATTCCAGTTTAAAATCGAGCgcgg                                                                                       <  2:97478/251‑4 (MQ=255)                                                                                                                                                     cGACCTTTAGCCTGGGCAAAAAA‑GACG‑CCGTTTTTAAAGCCGTGCTCGCGCC‑ACTCCAGCTCACTGTTATCGC‑GCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCgcgga                                                                                      <  1:228499/251‑1 (MQ=255)                                                                                                                                                           ttaaccTGGGCAAAAAA‑GACG‑CCGTTTTTAAAGCCGTGCTCGCGCCCAC‑CCAGCTCACGGTTATCGC‑GCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCg                                                                                  <  2:462254/245‑1 (MQ=255)                                                                                                                                                              cccTGGGAAAAAAA‑G‑CGTCCGTTTTTAAAGCCTTGCTCGCGCC‑CCTCCATCTCACTGTTATCGC‑GCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGGTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTGCAAAATCGAGCGCGGATGCGTCGcc                                                                             <  2:478131/250‑1 (MQ=255)                                                                                                                                                                  gggCAAAAAA‑GACG‑CCGTTTTTAAAGCCGTGCTCGCGCC‑ACTCCAGCTCACTGTTATCGC‑GCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAAACAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGACGCCATtc                                                                         >  1:731196/1‑251 (MQ=255)                                                                                                                                                                    gCAAAAAA‑GACG‑CCGTTTTTAAAGCCGTGCTCGCGCC‑ACTCCAGCTCACTGTTATCGC‑GCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATtctc                                                                       >  2:515522/1‑251 (MQ=255)                                                                                                                                                                       aaaaa‑GACG‑CCGTTTTTAAAGCCGTGCTCGCGCC‑ACTCCAGCTCACTGTTATCGC‑GCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATtctctgg                                                                    >  1:599190/1‑248 (MQ=255)                                                                                                                                                                          aa‑GACG‑CCGTTTTTAAAGCCGTGCTCGCGCC‑ACTCCAGCTCACTGTTATCGC‑GCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGccc                                                                 >  2:227137/1‑251 (MQ=255)                                                                                                                                                                                  ccGTTTTTAAAGCCGTGCTCGCGCC‑ACTCCAGCTCACTGTTATCGC‑GCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCCTGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGCGGCGGCCCCATTATCTGGCCCCAGcct                                                           >  2:672799/1‑249 (MQ=255)                                                                                                                                                                                     tttttAAAGCCGTGCTCGCGCC‑ACTCCAGCTCACTGTTATCGC‑GCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCatat                                                         >  1:516656/1‑250 (MQ=255)                                                                                                                                                                                     tttttAAAGCCGTGCTCGCGCC‑ACTCCAGCTCACTGTTATCGC‑GCCCCAGCCGCAGCGCAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCatat                                                         >  1:447486/1‑250 (MQ=255)                                                                                                                                                                                       tttAAAGCCGTGCTCGCGCC‑ACTCCAGCTCACTGTTATCGC‑GCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATaa                                                       >  1:288511/1‑250 (MQ=255)                                                                                                                                                                                          aaaGCCGTGCTCGCGCC‑ACTCCAGCTCACTGTTATCGC‑GCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGAATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCCCTGGCCCCAGCCTACAAACg                                                    >  2:948984/1‑250 (MQ=255)                                                                                                                                                                                              ccGTGCTCGCGCC‑ACTCCAGCTCACTGTTATCGC‑GCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGACGCCCTTCTCTGGCCCCAGCATAAAAACGATggg                                               >  2:542203/1‑249 (MQ=255)                                                                                                                                                                                                 tGCTCGCGCC‑ACTCCAGCTCACTGTTATCGC‑GCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTg                                            >  1:213774/1‑251 (MQ=255)                                                                                                                                                                                                  gcactcgcC‑CCTCCATCTCACTGTTATCTC‑GCCCCAGCCGCAGCTGAATACTGTAACGCTCGGCATGTTTTTGCAGCATTCGCATATCGAACTGCACCCCGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAGCGATGCGCTg                                            <  1:212058/245‑1 (MQ=255)                                                                                                                                                                                                    ccgcgCC‑CCTCCAGCTCACTGTTATCGC‑GCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGccc                                         <  1:104840/250‑1 (MQ=255)                                                                                                                                                                                                     cgcgCC‑ACTCCAGCTCACTGTTATCGC‑GCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCg                                        <  1:746808/251‑1 (MQ=255)                                                                                                                                                                                                     cgcgCC‑ACTCCAGCTCACTGTTATCGC‑GCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCg                                        >  1:868290/1‑251 (MQ=255)                                                                                                                                                                                                        gcC‑ACTCCAGCTCACTGTTATCGC‑GCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAg                                     <  1:892895/251‑1 (MQ=255)                                                                                                                                                                                                          c‑ACTCCAGCTCACTGTTATAGC‑GCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCAGATGGCGACGTAATCCAGTTCAAAAACGAGCGCGGAGGCGTCGCCATTCGCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGcc                                   >  2:570391/1‑251 (MQ=255)                                                                                                                                                                                                             cTCCAGCTCACTGTTATCGC‑GCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATACACGATGCGCAGCCCGCATCCtt                                 >  2:820948/1‑251 (MQ=255)                                                                                                                                                                                                               ccagcgcactgttatCGC‑GCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCtt                                 <  1:297300/238‑1 (MQ=255)                                                                                                                                                                                                               ccAGCTCACTGTTATCGC‑GCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCTGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCtt                                 <  1:429466/249‑1 (MQ=255)                                                                                                                                                                                                               ccAGCTCACTGTTATCGC‑GCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGGAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCtt                                 <  1:86245/249‑1 (MQ=255)                                                                                                                                                                                                                cagccccctgttatCGC‑CCCCCAGCCGCATCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGACGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCa                              <  2:288511/241‑1 (MQ=255)                                                                                                                                                                                                                cAGCTCACTGTTATCGC‑GCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCa                              <  1:908327/251‑1 (MQ=255)                                                                                                                                                                                                                    tcactgttatCGC‑GCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGcc                          >  2:235742/7‑251 (MQ=255)                                                                                                                                                                                                                       ctgttatCGC‑GCCCCAGACGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCACCCGATGATCACATCGGGATCGTAGTTGGCAAACCCGGCGTTGAGTTTTTCCAATAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCACAATCGCGCGCGGAGGCCTCGCCCTTCTCTGGCACCAGAATTTACACGATCCGCTACCCGACGCCTTCAAGGCCGAt                       >  2:581515/4‑251 (MQ=255)                                                                                                                                                                                                                         gttatCGC‑GCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGAt                       <  1:235742/248‑1 (MQ=255)                                                                                                                                                                                                                              cgc‑gCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGAGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTAc                >  1:728617/1‑251 (MQ=255)                                                                                                                                                                                                                                  gccccagccGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGAAGCCTTCCAGGCCGATGCAGTACAGc             >  2:787542/9‑251 (MQ=255)                                                                                                                                                                                                                                     ccagccGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTc           >  1:527515/6‑250 (MQ=255)                                                                                                                                                                                                                                      cagccGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACCGCTCAc         >  1:325352/5‑251 (MQ=255)                                                                                                                                                                                                                                             aGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCg  >  1:118419/1‑251 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                    AAACGGCGGTCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAGCTCCTGAGCGACAGTT‑TCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAA‑GACG‑CCGTTTTTAAAGCCGTGCTCGCGCC‑ACTCCAGCTCGCTATTATCGC‑GCCCAAGACGCAGCGGAAGACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGCAACTGCGGGCGGCTGGCGACGTATTCCAGTTCGAAATCAAGCGAGGAGGCGTCGCCATTCTCCGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCG  >  NC_000913/64819‑65300

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑