Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	65,046	A→C	R245R (CGT→CGG)	polB ←	DNA polymerase II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	65,046	0	A	C	95.8%	321.2 / ‑2.5	96	R245R (CGT→CGG)	polB	DNA polymerase II
Reads supporting (aligned to +/- strand):  ref base A (3/1);  new base C (45/47);  total (48/48)
Fisher's exact test for biased strand distribution p-value = 6.17e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01

CGCAGGTTTATCTTCGGCGAAACGGCGGTCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAGCTCCTGAGCGACAGTT‑TCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAA‑GACG‑CCGTTTTTAAAGCCGTGCTCGCGCC‑ACTCCAGCTCGCTATTATCGC‑GCCCAAGACGCAGCGGAAGACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGCAACTGCGGGCGGCTGGCGACGTATTCCAGTTCGAAATCAAGCGAGGAGGCGTCGCCATTCTCCGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTAC  >  NC_000913/64800‑65286                                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                 cgcAGGTTTATCTTCGGCGAAACGGCGGTCAATTTCGTCCATTAGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTT‑TCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAA‑GACG‑CCGTTTTTAAAGCCGTGCTCGCGCC‑ACTCCAGCTCACTGTTATCGC‑GCCCCAGCCGCa                                                                                                                                                                                                                                              <  1:887563/251‑1 (MQ=255)  gcAGGTTTATCTTCGGCGAAACGGCGGTCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTT‑TCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAA‑GACG‑CCGTTTTTAAAGCCGTGCTCGCGCC‑ACTCCAGCTCACTGTTATCGC‑GCCCCAGCCGCAg                                                                                                                                                                                                                                             <  1:935608/251‑1 (MQ=255)      gTTTATCTTCGGCGAAACGGCGGTCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTT‑TCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAA‑GACG‑CCGTTTTTAAAGCCGTGCTCGCGCC‑ACTCCAGCTCACTGTTATCGC‑GCCCCAGCCGCAGCGGa                                                                                                                                                                                                                                         <  1:351493/251‑1 (MQ=255)          aTCTTCGGCGAAACGGCGGTCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTT‑TCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAA‑GACG‑CCGTTTTTAAAGCCGTGCTCGCGCC‑ACTCCAGCTCACTGTTATCGC‑GCCCCAGCCGCAGCGGaata                                                                                                                                                                                                                                      <  1:339578/250‑3 (MQ=255)          aTCTTCGGCGAAACGGCGGTCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTT‑TCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAA‑GACG‑CCGTTTTTAAAGCCGTGCTCGCGCC‑ACCCCAGCTCACTGTTATCGC‑GCCCCAGCCGCAGCgggata                                                                                                                                                                                                                                      >  1:120250/1‑246 (MQ=255)                    aaaCGTCGGTCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTT‑TCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAA‑GACG‑CCGTTTTTAAAGCCGTGCTCGCGCC‑ACTCCAGCTCACTGTTATCGC‑GCCCCAGCCGCAGCGGAATACGGTAACGCTc                                                                                                                                                                                                                           <  2:171737/251‑1 (MQ=255)                    aaaCGGCGGTCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTT‑TCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAA‑GACG‑CCGTTTTTAAAGCCGTGCTCGCGCC‑ACTCCAGCTCACTGTTATCGC‑GCCCCAGCCGCAGCGGAATACGGTAACGCTc                                                                                                                                                                                                                           <  1:103569/251‑1 (MQ=255)                    aaaCGGCGGTCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTT‑TCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAA‑GACG‑CCGTTTTTAAAGCCGTGCTCGCGCC‑ACTCCAGCTCACTGTTATCGC‑GCCCCAGCCGCAGCGGAATACGGTAACGCTc                                                                                                                                                                                                                           >  1:305255/1‑251 (MQ=255)                        ggcggTCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTT‑TCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAA‑GACG‑CCGTTTTTAAAGCCGTGCTCGCGCC‑ACTCCAGCTCACTGTTATCGC‑GCCCCAGCCGCAGCGGAATACGGTAACGATCGGc                                                                                                                                                                                                                        >  2:798476/1‑250 (MQ=255)                         gcggTCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTT‑TCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAA‑GACG‑CCGTTTTTAAAGCCGTGCTCGCGCC‑ACTCCAGCTCACTGTTATCGC‑GCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCAt                                                                                                                                                                                                                      <  1:930517/251‑1 (MQ=255)                           ggTCAATTTCGTCCATTCGATCCCACGGGTTATCTATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTT‑TCCAGCGAGAATGAAGAGAAAGTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAA‑GACG‑CCGTTTTTAAAGCCGTGCTCGCGCC‑ACTCCAGCTCACTGTTATCGC‑GCCCCAGCCGCAGCGGAATACgg                                                                                                                                                                                                                                   <  2:548157/236‑1 (MQ=255)                           ggTCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTT‑TCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAA‑GACG‑CCGTTTTTAAAGCCGTGCTCGCGCC‑ACTCCAGCTCACTGTTATCGC‑GCCCCAGCCGCAGCGGAATACgg                                                                                                                                                                                                                                   >  1:548157/1‑236 (MQ=255)                           agTCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCTCCTAATAACTCCTGAGCGACGGTT‑TCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGACGATAATTAGCCGACCTTTAGCCTGGGCAAAACA‑GACG‑CCGTTTTTAAAGCCGGGCTCGCGCC‑ACACCAACTCACTGTTATCGC‑GCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGt                                                                                                                                                                                                                    >  2:787095/2‑251 (MQ=255)                              cAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTT‑TCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAA‑GACG‑CCGTTTTTAAAGCCGTGCTCGCGCC‑ACTCCAGCTCACTGTTATCGC‑GCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGttt                                                                                                                                                                                                                  <  1:660940/250‑1 (MQ=255)                                    cGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTT‑TCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAA‑GACG‑CCGTTTTTAAAGCCGTGCTCGCGCC‑ACTCCAGCTCACTGTTATCGC‑GCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTgcag                                                                                                                                                                                                            <  1:389720/250‑1 (MQ=255)                                       ccATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTT‑TCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAA‑GACG‑CCGTTTTTAAAGCCGTGCTCGCGCC‑ACTCCAGCTCACTGTTATCGC‑GCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCAtt                                                                                                                                                                                                        >  1:72330/1‑251 (MQ=255)                                       ccATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTT‑TCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAA‑GACG‑CCGTTTTTAAAGCCGTGCTCGCGCC‑ACTCCAGCTCACTGTTATCGC‑GCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCAtt                                                                                                                                                                                                        >  2:297300/1‑251 (MQ=255)                                        catccgaccccACGGGTTACCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTT‑TCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGCCTATAATTAGCCGACCTTTAGCCTGGGCAAAAAA‑GACG‑CCGTTTTTAAAGCCGTGCTCGCGCC‑ACTCCAGCTCACTGTTATCGC‑GCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTc                                                                                                                                                                                                       <  2:682494/243‑1 (MQ=255)                                         aTTCGATACCACGGGATATAGGTAGCTTTACCTTCGCCTAATAACTCCTGAGCGACGGTC‑TCCAGCGAGAATGAAGAGCAATTCCAGAACGCGGATTTCAGCGACTCGATACCATCGCTAATTAGCCGACCTTTCGCCTGGGCAAACAA‑GACG‑CCGTTTTTAAAGCCGTGCTCGCGCC‑ACTCCAGCTCACTGTTATCGCCACCCATCA‑GCAGCGGCATACGGTACCGCTCGGAATGtttttccggcatacg                                                                                                                                                                                                      >  2:410925/1‑241 (MQ=255)                                            cgaccccACGGGTTATCGTTAGATGTTCCTGCGCCTAAGAACTCCTGTGCGACGGTT‑TCCAGCGAGAATGAAGAGAAATTCCAGACCGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAA‑GACG‑CCGTTTTTAAAGCCGTGCTCGCGCC‑ACTCCAGCTCACTGTTATCGC‑GCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAg                                                                                                                                                                                                   <  2:954131/247‑1 (MQ=255)                                            cGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTT‑TCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAA‑GACG‑CCGTTTTTAAAGCCGTGCTCGCGCC‑ACTCCAGCTCACTGTTATCGC‑GCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCa                                                                                                                                                                                                    <  1:840889/250‑1 (MQ=255)                                             gATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTT‑TCCAGAGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAA‑GACG‑CCGTTTTTAAAGCCGTGCTCGCGCC‑ACTCCAGCTCACTGTTATCGC‑GCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGc                                                                                                                                                                                                     >  1:509447/1‑248 (MQ=255)                                              aTCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGAC‑GTTCTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATCCCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAA‑GACG‑CCGTTTTTAAAGCCGTGCTCGCGCC‑ACTCCAGCTCACTGTTATCGC‑GTCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGAt                                                                                                                                                                                                 <  1:406753/251‑1 (MQ=255)                                                cccACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTT‑TCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAA‑GACG‑CCGTTTTTAAAGCCGTGCTCGCGCC‑ACTCCAGCTCACTGTTATCGC‑GCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGc                                                                                                                                                                                                     >  1:327960/1‑245 (MQ=255)                                                  cACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTT‑TCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAA‑GACG‑CCGTTTTTAAAGCCGTGCTCGCGCC‑ACTCCAGCTCACTGTTATCGC‑ACCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTATTGCAGCATTCgacgaaatg                                                                                                                                                                                              >  2:153279/1‑242 (MQ=255)                                                         tATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTT‑TCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAA‑GACG‑CCGTTTTTAAAGCCGTGCTCGCGCC‑ACTCCAGCTCACTGTTATCGC‑GCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTGTGCAGCATTCGCAGATCGAACTGcac                                                                                                                                                                                       <  1:788722/250‑1 (MQ=255)                                                         tATCGATAGATTTTCCTGCGCCGAATAACCCCTGAGCGACGGTT‑TCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAA‑GACG‑CCGTTTTTAAAGCCGTGCTCGCGCC‑ACTCCAGCTCACTGTTATCGC‑GCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGcaa                                                                                                                                                                                       <  2:228135/250‑2 (MQ=255)                                                            cGATAGATTTTCATTCGCCTAATAACTCCTGAGCGACGGTT‑TCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAA‑GACG‑CCGTTTTTAAAGCCGTGCTCGCGCC‑ACTCCAGCTCACTGTTATCGC‑GCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACg                                                                                                                                                                                   >  1:741151/1‑251 (MQ=255)                                                                        cTTCGCCTAATAGCTCCTGAGCGACAGTT‑TCCAGCGAGAATGAAGAGAAATTCTAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAA‑GACG‑CCGTTTTTAAAGCCGTGCTCGCGCC‑ACTCCAGCTCGCTATTATCGC‑GCCCAAGACGCAGCGGAAGACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGt                                                                                                                                                                                  >  1:682542/1‑240 (MQ=255)                                                                        cTTCGCCTAATAGCCCGTGAGCGACAGTT‑TCCAGCGAGAATGAAGAGAAATTCTAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGCCCTTTAGCCTGGGCAAAAAA‑GACG‑CCGTTTTTAAAGCCGTGCTCGCGCC‑ACTCCAGCTCGCTATTATCGC‑GCCCAAGACGCAGCGGAAGACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGt                                                                                                                                                                                  <  2:682542/240‑1 (MQ=255)                                                                           cGCCTAAAAACTCCTGAGCTACGGTT‑TCCAGCGAGAATGAAGAGAAATTCCAGAACTCGGATTTCAGCGCCCCGATACCGTCGATAATTAGCCGACCTCTAGCCTGGGCAAAAAA‑GACG‑CCGTTTTTAAAGCCGTGCTCGCGCC‑ACTCCAGCTCACTGTTATCGC‑GCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATc                                                                                                                                                                    <  2:183517/251‑1 (MQ=255)                                                                                  taacTCCTGAGCGACGGTT‑TCCATCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAACGCCTCGATACCGTCGATACTTAGCCGACCTTTAGCCTGGGCAAAAAA‑GACG‑CCGTTTTTAAAGCCGTGCTCGCGCC‑ACTCCAGCTCACTGTTATCGC‑GCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTCGCAGCATTCGCAGATCGCACTGCACCACTTTCCACCCgcgtatcacatc                                                                                                                                                               >  2:611922/4‑238 (MQ=255)                                                                                    acTCCTGAGCGACGGTT‑TCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAA‑GACG‑CCGTTTTTAAAGCCGTGCTCGCGCC‑ACTCCAGCTCACTGTTATCGC‑GCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAgg                                                                                                                                                            >  1:316078/2‑250 (MQ=255)                                                                                      tCCTGAGCGACGGTT‑TCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAA‑GACG‑CCGTTTTTAAAGCCGTGCTCGCGCC‑ACTCCAGCTCACTGTTATCGC‑GACCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCGTTCGCAGATCGAACTGCACCCCGTTCCAACCGAGGATAACATCAggccc                                                                                                                                                         >  2:282778/1‑248 (MQ=255)                                                                                       ccTGAGCGACGGTT‑TCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAA‑GACG‑CCGTTTTTAAAGCCGTGCTCGCGCC‑ACTCCAGCTCACTGTTATCGC‑GCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCg                                                                                                                                                        <  1:198777/251‑1 (MQ=255)                                                                                        cTGAGCGACGGTT‑TCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAC‑GACG‑CCGTTTTTAAAGCCGTGCTCGCGCC‑ACTCCAGCTCACTGTTATCGC‑GCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCCGGATCGt                                                                                                                                                       >  2:229483/1‑251 (MQ=255)                                                                                          gAGCGACGGTT‑TACAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAA‑GACG‑CCGTTTTTAAAGCCGTGCTCGCGCC‑ACTCCAGCTCACTGTTATCGC‑GCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAg                                                                                                                                                     <  1:676429/251‑1 (MQ=255)                                                                                              gacggTT‑TCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGCTTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAC‑GACG‑CCGTTTTTAAAGCCGTGCTCGCGCC‑ACTCCAGCTCACTGTTATCGC‑GCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGACCTGCACCACGTTCCAACCGATGATCACATCAGGCTCGTAGTTgg                                                                                                                                                 >  2:651521/5‑251 (MQ=255)                                                                                                           cGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATCCCGTCGATAATTAGCCGCCCTTTAGCCTGGGCAAAAAA‑GACG‑CCGTTTTTAAAGCCGTGCTCGCGCC‑ACTCCAGCTCACTGTTATCGC‑GCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCGTTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGt                                                                                                                                     <  1:695633/251‑1 (MQ=255)                                                                                                             agaATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAA‑GACG‑CCGTTTTTAAAGCCGTGCTCGCGCC‑ACTCCAGCTCACTGTTATCGC‑GCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTg                                                                                                                                   <  1:825712/251‑1 (MQ=255)                                                                                                              gaATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAA‑GACG‑CCGTTTTTAAAGCCGTGCTCGCGCC‑ACTCCAGCTCCCTGTTATCGC‑GCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCCCGTTCCAACCGATGATCCCATCAGGCTCGTGGTTGGCAAACCAGGCGTTgg                                                                                                                                  >  2:370960/1‑250 (MQ=255)                                                                                                                    agagAAATTCCAGAACGAGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAA‑GACG‑CCGTTTTTAAAGCCGTGCTCGCGCC‑ACTCCAGCTCACTGTTATCGC‑GCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGCGttttt                                                                                                                            >  2:919965/1‑251 (MQ=255)                                                                                                                     gagaAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAA‑GACG‑CCGTTTTTAAAGCCGTGCTCGCGCC‑ACTCCAGCTCACTGTTATCGC‑GCCCCAGCCGCAGCGGCATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCCCCAAGTTCCAACCGATGATCACATCAGGTTCGTAGTTGGCAAACCAGGCGTTGAGTTTTTc                                                                                                                           >  2:935836/1‑251 (MQ=255)                                                                                                                     gagaAAGTCCAGACCGCGGATTTCAGCGCCTCGATTCCGCTGATAATTAGCCGACCCTTATCCTGGGCAAAAAA‑GACG‑CCGTTTTTAAAGCCGTGCTCGCGCC‑ACTCCAGCTCACTGTTATCGC‑GCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGAGCGATCTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTc                                                                                                                           <  2:885295/251‑1 (MQ=255)                                                                                                                         aataccAGAACCCTGATTTCAGCGCCTCGATTCCGGAGATAATTATCCGCCCTTTAGCCTGGGCAAAAAA‑GACG‑CCGTGTTTAAAGCCGTGCTCGCGCC‑TCGCCAGCTCACTGTTATCGC‑GCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAgt                                                                                                                       <  2:361865/247‑2 (MQ=255)                                                                                                                          aTTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAA‑GACG‑CCGTTTTTAAAGCCGTGCTCGCGCC‑ACTCCAGCTCACTGTTATCGC‑GCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAgt                                                                                                                       >  1:246798/1‑249 (MQ=255)                                                                                                                            tCCAGAACGCGGATCTCAGCGCCCCGATACCGTCGATAATTAGCCCGCCTTGAGCCTGGGCAAAAAA‑GACT‑CCGATTTTAAAGCCGTGCTCGCGCC‑TCTCCAGCGCACTGTTATCGC‑GCCTCAGCCGCAGCGGAATACGGTAACGCTCGTCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAgtag                                                                                                                     <  2:789825/250‑4 (MQ=255)                                                                                                                                   cgcgGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAA‑GACG‑CCGTTTTTAAAGCCGTGCTCGCGCC‑ACTCCAGCTCACTGTTATCGC‑GCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTgcgggc                                                                                                             <  1:767449/251‑1 (MQ=255)                                                                                                                                     cgGATTTCAGCGCCTCGATACCGTCGATAATTAGCCTACCTTTAGCCTGGGCAAAAAA‑GACG‑CCGTTTTTAAAGCCGTGCTCGCGCC‑ACTCCAGCTCACTGTTATCGC‑GCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACAACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTgcgggcgg                                                                                                           <  1:618221/251‑1 (MQ=255)                                                                                                                                     cgGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAA‑GACG‑CCGTTTTTAAAGCCGTGCTCGCGCC‑ACTCCAGCTCACTGTTATCGC‑GCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTgcgggcgg                                                                                                           >  1:936929/1‑251 (MQ=255)                                                                                                                                       gttttCAGCGCCACGATACCGTCGATAATTAGCCGCCCTTTAGCCTGGGCAAAAAA‑GACG‑CCGTTTTTAAAGCCGTGCTCGCGCC‑ACTCCAGCTCACTGTTATCGC‑GCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCt                                                                                                         <  2:489983/249‑1 (MQ=255)                                                                                                                                           tCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAA‑GACG‑CCGTTTTTAAAGCCGTGCTCGCGCC‑ACTCCAGCTCACTGTTATCGC‑GCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCg                                                                                                     >  2:229987/1‑251 (MQ=255)                                                                                                                                             cgcgcCTCGTTACCGTCGATAATTAGCCGACCTTTCGCCTGGGCAAAAAA‑GACC‑CCGTTTTTAAAGCCGTGCTCGCGCC‑ACTCCAGCTCACTGTTATCGC‑GCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGAc                                                                                                   <  2:313509/250‑1 (MQ=255)                                                                                                                                             aGCGCCTCGATACCGACGATATTTAGCCGTCTTTTAGCCTGGGCAAAAAA‑GACG‑CCGTTTTTAAAGTTGTGCTCGCGCC‑ACTCCAGCTCGCTGTTATCGC‑GCTCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGACCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGTCGAc                                                                                                   <  1:611922/251‑1 (MQ=255)                                                                                                                                                gcCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAA‑GACG‑CCGTTTTTAAAGCCGTGCTCGCGCC‑ACTCCAGCTCACTGTTATCGC‑GCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGt                                                                                                 >  1:293319/1‑250 (MQ=255)                                                                                                                                                   tCGATACAGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAA‑GACG‑CCGTTTTTAAAGCCGTGCTCGCGCC‑ACTCCAGCTCACTGTTATCGC‑GCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCACCCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTc                                                                                             >  2:908327/1‑251 (MQ=255)                                                                                                                                                    cGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAA‑TACG‑CCGTTTTTAAAGCCGTGCTCGCGCC‑ACTCCAGCTCACTGTTATCGC‑GCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTc                                                                                             <  1:387540/250‑1 (MQ=255)                                                                                                                                                    cGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAA‑GACG‑CCGTTTTTAAAGCCGTGCTCGCGCC‑ACTCCAGCTCACTGTTATCGC‑GCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTcc                                                                                            <  1:798476/251‑1 (MQ=255)                                                                                                                                                     gATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAA‑GACG‑CCGTTTTTAAAGCCGTGCTCGCGCC‑ACTCCAGCTCACTGTTATCGC‑GCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCa                                                                                           <  1:556249/251‑1 (MQ=255)                                                                                                                                                          cGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAA‑GACG‑CCGTTTTTAAAGCCGTGCTCGCGCC‑ACTCCAGCTCACTGTTATCGC‑GCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTc                                                                                       >  1:223311/1‑250 (MQ=255)                                                                                                                                                            ccGATAATTAGCCGACCTTTAGCCTGGGCAAAAAA‑GACG‑CCGTTTTTAAAGCCGTGCTCGCGCC‑ACTCCAGCTCACTGTTATCGC‑GCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTcaaa                                                                                    <  1:587659/250‑4 (MQ=255)                                                                                                                                                             cGATAATTAGCCGACCTTTAGCCTGGGAAAAAAA‑GACG‑CCGTTTTTAAGGCCGTGCTCGCGCC‑ACTCCAGCTCCCTGTTATCGC‑GCCACAGCCGCAGCGGAATACGGTAACGCTGGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACAACGTTACAACCGATGCTCACATCAGGGTCGTAGTTGGCAAACCAGGCGATGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGttaaaa                                                                                    >  2:581017/1‑246 (MQ=255)                                                                                                                                                              gATAATTAGCCGACCTTTAGCCTGGGCAAAAAA‑GACG‑CCGTTTTTAAAGCCGTGCTCGCGCC‑ACTCCAGCTCACTGTTATCGC‑GCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAAt                                                                                  >  1:799297/1‑251 (MQ=255)                                                                                                                                                                  aTTAGCCGACCTTTAGCCTGGGCAAAAAA‑GACG‑CCGTTTTTAAAGCCGTGCTCGCGCC‑ACTCCAGCTCACTGTTATCGC‑GCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATcg                                                                                >  1:545960/1‑248 (MQ=255)                                                                                                                                                                     aGCCGACCTTTAGCCTGGGCAAAAAA‑GCCG‑CCGTTTTTAAAGCCGTGCTCGCGCC‑ACTCCAGCTCACTGTTATCGC‑GCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCgc                                                                           >  1:330562/1‑250 (MQ=255)                                                                                                                                                                       ccGACCTTTGGCCGGGGCAAAAAATGA‑G‑CCGTTTTTAAAGCAGTGCCCGCGCC‑ACGCCAGCTCACTGTTAACGC‑GCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTGCCAACCGATGATCACAGCAGGATCTTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGGAGCTGCGGGCGTCTGGCGACGTATTCCAGTTTAAAATCGAGCgcgg                                                                         <  2:97478/251‑4 (MQ=255)                                                                                                                                                                        cGACCTTTAGCCTGGGCAAAAAA‑GACG‑CCGTTTTTAAAGCCGTGCTCGCGCC‑ACTCCAGCTCACTGTTATCGC‑GCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCgcgga                                                                        <  1:228499/251‑1 (MQ=255)                                                                                                                                                                              ttaaccTGGGCAAAAAA‑GACG‑CCGTTTTTAAAGCCGTGCTCGCGCCCAC‑CCAGCTCACGGTTATCGC‑GCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCg                                                                    <  2:462254/245‑1 (MQ=255)                                                                                                                                                                                 cccTGGGAAAAAAA‑G‑CGTCCGTTTTTAAAGCCTTGCTCGCGCC‑CCTCCATCTCACTGTTATCGC‑GCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGGTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTGCAAAATCGAGCGCGGATGCGTCGcc                                                               <  2:478131/250‑1 (MQ=255)                                                                                                                                                                                     gggCAAAAAA‑GACG‑CCGTTTTTAAAGCCGTGCTCGCGCC‑ACTCCAGCTCACTGTTATCGC‑GCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAAACAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGACGCCATtc                                                           >  1:731196/1‑251 (MQ=255)                                                                                                                                                                                       gCAAAAAA‑GACG‑CCGTTTTTAAAGCCGTGCTCGCGCC‑ACTCCAGCTCACTGTTATCGC‑GCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATtctc                                                         >  2:515522/1‑251 (MQ=255)                                                                                                                                                                                          aaaaa‑GACG‑CCGTTTTTAAAGCCGTGCTCGCGCC‑ACTCCAGCTCACTGTTATCGC‑GCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATtctctgg                                                      >  1:599190/1‑248 (MQ=255)                                                                                                                                                                                             aa‑GACG‑CCGTTTTTAAAGCCGTGCTCGCGCC‑ACTCCAGCTCACTGTTATCGC‑GCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGccc                                                   >  2:227137/1‑251 (MQ=255)                                                                                                                                                                                                     ccGTTTTTAAAGCCGTGCTCGCGCC‑ACTCCAGCTCACTGTTATCGC‑GCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCCTGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGCGGCGGCCCCATTATCTGGCCCCAGcct                                             >  2:672799/1‑249 (MQ=255)                                                                                                                                                                                                        tttttAAAGCCGTGCTCGCGCC‑ACTCCAGCTCACTGTTATCGC‑GCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCatat                                           >  1:516656/1‑250 (MQ=255)                                                                                                                                                                                                        tttttAAAGCCGTGCTCGCGCC‑ACTCCAGCTCACTGTTATCGC‑GCCCCAGCCGCAGCGCAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCatat                                           >  1:447486/1‑250 (MQ=255)                                                                                                                                                                                                          tttAAAGCCGTGCTCGCGCC‑ACTCCAGCTCACTGTTATCGC‑GCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATaa                                         >  1:288511/1‑250 (MQ=255)                                                                                                                                                                                                             aaaGCCGTGCTCGCGCC‑ACTCCAGCTCACTGTTATCGC‑GCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGAATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCCCTGGCCCCAGCCTACAAACg                                      >  2:948984/1‑250 (MQ=255)                                                                                                                                                                                                                 ccGTGCTCGCGCC‑ACTCCAGCTCACTGTTATCGC‑GCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGACGCCCTTCTCTGGCCCCAGCATAAAAACGATggg                                 >  2:542203/1‑249 (MQ=255)                                                                                                                                                                                                                    tGCTCGCGCC‑ACTCCAGCTCACTGTTATCGC‑GCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTg                              >  1:213774/1‑251 (MQ=255)                                                                                                                                                                                                                     gcactcgcC‑CCTCCATCTCACTGTTATCTC‑GCCCCAGCCGCAGCTGAATACTGTAACGCTCGGCATGTTTTTGCAGCATTCGCATATCGAACTGCACCCCGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAGCGATGCGCTg                              <  1:212058/245‑1 (MQ=255)                                                                                                                                                                                                                       ccgcgCC‑CCTCCAGCTCACTGTTATCGC‑GCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGccc                           <  1:104840/250‑1 (MQ=255)                                                                                                                                                                                                                        cgcgCC‑ACTCCAGCTCACTGTTATCGC‑GCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCg                          >  1:868290/1‑251 (MQ=255)                                                                                                                                                                                                                        cgcgCC‑ACTCCAGCTCACTGTTATCGC‑GCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCg                          <  1:746808/251‑1 (MQ=255)                                                                                                                                                                                                                           gcC‑ACTCCAGCTCACTGTTATCGC‑GCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAg                       <  1:892895/251‑1 (MQ=255)                                                                                                                                                                                                                             c‑ACTCCAGCTCACTGTTATAGC‑GCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCAGATGGCGACGTAATCCAGTTCAAAAACGAGCGCGGAGGCGTCGCCATTCGCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGcc                     >  2:570391/1‑251 (MQ=255)                                                                                                                                                                                                                                cTCCAGCTCACTGTTATCGC‑GCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATACACGATGCGCAGCCCGCATCCtt                   >  2:820948/1‑251 (MQ=255)                                                                                                                                                                                                                                  ccagcgcactgttatCGC‑GCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCtt                   <  1:297300/238‑1 (MQ=255)                                                                                                                                                                                                                                  ccAGCTCACTGTTATCGC‑GCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCTGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCtt                   <  1:429466/249‑1 (MQ=255)                                                                                                                                                                                                                                  ccAGCTCACTGTTATCGC‑GCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGGAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCtt                   <  1:86245/249‑1 (MQ=255)                                                                                                                                                                                                                                   cagccccctgttatCGC‑CCCCCAGCCGCATCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGACGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCa                <  2:288511/241‑1 (MQ=255)                                                                                                                                                                                                                                   cAGCTCACTGTTATCGC‑GCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCa                <  1:908327/251‑1 (MQ=255)                                                                                                                                                                                                                                       tcactgttatCGC‑GCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGcc            >  2:235742/7‑251 (MQ=255)                                                                                                                                                                                                                                          ctgttatCGC‑GCCCCAGACGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCACCCGATGATCACATCGGGATCGTAGTTGGCAAACCCGGCGTTGAGTTTTTCCAATAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCACAATCGCGCGCGGAGGCCTCGCCCTTCTCTGGCACCAGAATTTACACGATCCGCTACCCGACGCCTTCAAGGCCGAt         >  2:581515/4‑251 (MQ=255)                                                                                                                                                                                                                                            gttatCGC‑GCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGAt         <  1:235742/248‑1 (MQ=255)                                                                                                                                                                                                                                                 cgc‑gCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGAGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTAc  >  1:728617/1‑251 (MQ=255)                                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                 CGCAGGTTTATCTTCGGCGAAACGGCGGTCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAGCTCCTGAGCGACAGTT‑TCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAA‑GACG‑CCGTTTTTAAAGCCGTGCTCGCGCC‑ACTCCAGCTCGCTATTATCGC‑GCCCAAGACGCAGCGGAAGACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGCAACTGCGGGCGGCTGGCGACGTATTCCAGTTCGAAATCAAGCGAGGAGGCGTCGCCATTCTCCGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTAC  >  NC_000913/64800‑65286

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑