Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 74,308 T→C I72V (ATA→GTA)  thiP ← thiamine ABC transporter membrane subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091374,3080TC97.5% 362.3 / ‑0.4 123I72V (ATA→GTA) thiPthiamine ABC transporter membrane subunit
Reads supporting (aligned to +/- strand):  ref base T (2/1);  new base C (62/56);  total (66/57)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.43e-01

Only 100 of 123 total aligned reads displayed.

CATCGGCAGATTAAAAAACACATGGGCCAGCAAAATACCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGTGTTGCCAGCCAGCCC‑TGGCGACCATAGACGCTAAGA‑ATGCCGAAAACAGCGACCAACACCGGGAGGATCAAGGTCATTGCACACAGACGCAACAGCGCCAGCCGACCCGGAAAGCGCCTGCGATAGAGCGCGCGGGCGAGGAATATCGCGGGTATGACAGAGAGCAGTGCCGAGAGAAACGCCTGCCAGAAGGAGAAGCGCACCACATGCCAC‑AGATAGCTGTCCTGCCAGACTGCCACCCAGTCATCCTGCGGCGCGTTCCACCACAGGGCGAGAAACGCCGCCAGCGCAACCGCTACCACCAGCGTGGTGGCGCTTACACCTGGAATTAACCAGCCGGGAATTAACGGCTGACGGCGCGTTGCCATTCGCTAATCCATGCCTGACGTTGTGCCGCCAC  >  NC_000913/74063‑74553
                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                      
cATCGGTAGATTAAAAAATACGTGCGCCAGCAAAATACCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGTGTTGCCAGCCAGCCC‑TGGCGACCATAGACGCTAAGA‑ATGCCGAAAACAGCAACCAGCACCGGGAGGATTAAGGTCATTGCGCACAGACGCAACAGCGCCAGCCGACCCGGAAAGCGCCTGCGATAGAGCGCGCGGGCGAGGAATATCGCGGGTACGACag                                                                                                                                                                                                                                                   <  1:817631/251‑1 (MQ=255)
 aTCGGTAGATTAAAAAATACGTGCGCCAGCAAAATACCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGTGTTGCCAGCCAGCCC‑TGGCGACCATAGACGCTAAGA‑ATGCCGAAAACAGCAACCAGCACCGGGAGGATTAAGGTCATTGCGCACAGACGCAACAGCGCCAGCCGACCCGGAAAGCGCCTGCGCTAGAGCGCGCGGGCGAGGCATATCGCGGGTACGAc                                                                                                                                                                                                                                                     >  1:874596/1‑248 (MQ=255)
   cggtaGATTAAAAAATACGTGCGCCAGCAAAATACCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGTGTGGCCAGCCAGCCC‑TGGCGACCATAGACGCTAAGA‑ATGCCGAAAACAGCAACCAGCACCGGGAGGATTAAGGTCATTGCGCACAGACGCAACAGCGCCAGCCGACCCGGAAAGCGCCTGCGATAGAGCGCGCGGGCGAGGAATATCGCGGGTACGACagaga                                                                                                                                                                                                                                                <  1:544587/247‑1 (MQ=255)
         ataaaaaaaTACGTGCGCCAGCAAAATACCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGTGTTGCCAGCCAGCCC‑TGGCGACCAGAGACGCTAAGA‑ATGCCGAAAACAGCAACCAGCACCGGGAGGATTAAGGTCATTGCGCACAGACGCAACAGCGCCAGCCGACCCGGAAAGCGCCTGCGATAGAGCGCGCGGGCGAGGAATATCGCGGGTACGACAGAGAGCAgcg                                                                                                                                                                                                                                          <  2:172981/248‑3 (MQ=255)
             aaaaatacgtgcgCCAGCAAAATACCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGTGTTGCCAGCCAGCCC‑TGGCGACCATAGACGCTAAGA‑ATGCCGAAAACAGCAACCAGCACCGGGAGGATTAAGGTCATTGCGCACAGACGCAACAGCGCCAGCCGACCCGGAAAGCGCCTGCGATAGAGCGCGCGGGCGAGGAATATCGCGGGTACGACAGAGAGCAGCGCCg                                                                                                                                                                                                                                       >  1:763717/13‑250 (MQ=255)
                  accgtgcgccagcaaacacccatGCAGTCCGTAGGGCAAAAAGGCCCACTCCAGACCGAGCGATTGGCAGATTGTTGCCAGCCAGCCC‑TGGCGACCATAGACGCTAAGA‑ATGCCGAAAACAGCAACCAGCACCGGGAGGATTAAGGGCATTGCGCACAGACGCAACAGCGCCAGCCGACCCGGAAAGCGCCTGCGATAGAGCGCGCGGGCTAGGAATAGCGCTGGTACGACAGATAGCAGCGTCGAGAGaa                                                                                                                                                                                                                                 <  2:679724/229‑1 (MQ=255)
                   acgtgcgCCAGCAAAATACCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGTGTTGCCAGCCAGCCC‑TGGCGACCATAGACGCTAAGA‑ATGCCGAAAACAGCAACCAGCACCGGGAGGATTAAGGTCATTGCGCACAGACGCAACAGCGCCAGCCGACCCGGAAAGCGCCTGCGATAGAGCGCGCGGGCGAGGAATATCGCGGGTACGACAGAGAGCAGCGCCGAGAGaaa                                                                                                                                                                                                                                <  1:693492/245‑1 (MQ=255)
                    cgtgcgCCAGCAAAATACCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGTGTTGCCAGCCAGCCC‑TGGCGACCATAGACGCTAAGA‑ATGCCGAAAACAGCAACCAGCACCGGGAGGATTAAGGTCATTGCGCACAGACGCAACAGCGCCAGCCGACCCGGAAAGCGCCTGCGATAGAGCGCGCGGGCGAGGAATATCGCGGGTACGACAGAGAGCAGCGCCGAGAGAAAc                                                                                                                                                                                                                               >  1:894644/6‑251 (MQ=255)
                    cgtgagCCAGCAAAATACCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGCGTGTTGCCAGCCAGCCC‑TGGCGAACATAGACGCTAAGA‑ATGCCGAAAACAGCAACCAGCACCGGGAGGATTAAGGTCATTGCGCACAGACGCAACAGCGCCAGCCGCGCCGGAAAGCGCCTGCGATCGAGCGCGCGGGCCAGGAGTATCGCGGGTGCGACAGAGCGCAGCGCCGCGAGaacc                                                                                                                                                                                                                               >  2:313256/6‑249 (MQ=255)
                    caTGGGCCAGCAAAATACCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGTGTTGCCAGCCAGCCC‑TGGCGACCATAGACGCTAAGA‑ATGCCGAAAACAGCGACCAACACCGGGAGGATCAAGGTCATTGCACACAGACGCAACAGCGCCAGCCGACCCGGAAAGCGCCTGCGATAGAGCGCGCGGGCGAGGAATATCGCGGGTATGACAGAGAGCAGTGCCGAGAGAAAc                                                                                                                                                                                                                               >  1:269521/1‑251 (MQ=255)
                     gtgcgcccgaaaaaTAACTTGCAGGACGTAGGGCGGAAAGGTCCACTCCCGACCGAGCGATTGGCAGAGTGTTGCCAGCCAGCCC‑TGGCGACCATAGACGCTAAGA‑ATGCCGAAAACAGCAACCAGCACCGGGTGGATTACGGTCATTGCGCCCAGACGCAACAGCGCCAGCCGACCCGAATAGCGCCTGCGGAAGAGCGCGCGGGCGGGGAATATCGCGGTTACGACAGAGAGCAGTGACGAGAGAAACg                                                                                                                                                                                                                              >  2:475908/11‑251 (MQ=255)
                       gcgCCAGCAAAATACCTTGCAGGCCGTGGGGCGCAAAGGGCCACTCCAGACCGAGCGATTGGCAGAGTGTTGCCAGCCAGCCC‑GGTCGACCATAGCCGCTAAGA‑ATGCCGAAAACAGCAACCACCACCGGGAGGATTAAGGGCATTGCTCACAGACGCAACAGCGCCAGCCCACCCGGAAAGCGCCTGCGATAGAGCGCGCGGGCGAGGAAGATCGCGGGTACGACAGAGAGCAGCGCCGATAGAAACGcc                                                                                                                                                                                                                            <  2:498626/249‑1 (MQ=255)
                        cgCCAGCAAAATACCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGCGTGTTGCCAGCCAGCCC‑TGGCGACCATAGCCGCTAAGA‑ATGCCGAAAACAGCAACCAGCACCGGGAGGATTAAGGTCATTGCGCACAGACGCAACAGCGCCAGCCGACCCGGAAAGCGCCTGCGATAGAGCGCGCGGGCGAGGAATATCGCGGGTCCGACAGAGAACAGCGACGAGAaaaaagacc                                                                                                                                                                                                                           >  2:736176/2‑246 (MQ=255)
                        cgCCAGCAAAATACCTTGCAGGCCGTAAGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGTGTTGCCAGCCAGCCC‑TGGCGACCATAGACGCTAAGA‑ATGCCGAAAACAGCAACCAGCACCGGGAGGATTAAGGTCATTGCGCACAGACGCAACAGCGCCAGCCGACCCGGAAAGCGCCTGCGCTAGAGCGCGCGGGCGAGGAATATCGCGGGTACGACAGAGAGCAGCGCCGAGAGAAACGCCt                                                                                                                                                                                                                           >  1:165027/2‑251 (MQ=255)
                        cgCCAGCAAAAAACCTTGCAGGCCGTCGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGAGTTGCCAGCCAGCCC‑TGGCGACCATAGACGCTAAGA‑ATGCCGAAAACAGCAACCAGCACCGGGAGGGTTAAGGTCATTGCGCACAGACGCAACAGCGCAAGCCGACCCGGAAAGAGCCTGCGATAAAGCGCGCGGGCGAGGAATATCGCGGGTACGACAGAGAGAAGCGCCGCGAGAAACGccc                                                                                                                                                                                                                           >  1:393125/2‑250 (MQ=255)
                         gCCAGCAAAATACCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGTGTTGCCAGCCAGCCC‑TGGCGACCATAGACGCTAAGA‑ATGCCGAAAACAGCAACCAGCACCGGGAGGATTAAGGTCATTGCGCACAGACGCAACAGCGCCAGCCGACCCGGAAAGCGCCTGCGATAGAGCGCGCGGGCGAGGAATATCGCGGGTACGACAGAGAGCAGCGCCGAGAGAAACGCCTg                                                                                                                                                                                                                          <  1:65474/251‑1 (MQ=255)
                         gCCAGAAAAATCCCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGGGTTGCCAGCCAGCCC‑TGGCGACCATAGACGCTAAGA‑ATGCCGAAAACAGCAACCAGCACCGGGAGGATTAAGGTCATTGCGCACAGACGCAACAGCGCCAGCCGACCCGGAAAGCGCCTGCGATAGAGCGCGCGGGCGAGGAATATCGCGGGTACGACAGAGAGCAGCGCCGAGAGAAACGCCTg                                                                                                                                                                                                                          <  2:901332/251‑1 (MQ=255)
                           cAGCAAAATACCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGTGTTGCCAGCCAGCCC‑TGGCGACCATAGACGCTAAGA‑ATGCCGAAAACAGCAACCAGCACCGGGAGGATTAAGGTCATTGCGCACAGACGCAACAGCGCCAGCCGACCCGGAAAGCGCCTGCGATAGAGCGCGCGGGCGAGGAATATCGCGGGTACGACAGAGAGCAGCGCCGAGAGAAACGCCTGcc                                                                                                                                                                                                                        <  1:163942/251‑1 (MQ=255)
                               aaaatcacgtGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGTGTTGCCAGCCAGCCC‑TGGCGACCATAGACGCTAAGA‑ATGCCGAAAACAGCAACCAGCACCGGGAGGATTAAGGTCATTGCGCACAGACGCAACAGCGCCAGCCGACCCGGAAAGCGCCTGCGATAGAGCGCGCGGGCGAGGAATATCGCGGGTACGACAGAGAGCAGCGCCGAGAGAAACGCCTGCCAGaa                                                                                                                                                                                                                    <  2:858674/242‑1 (MQ=255)
                                  aTACCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGTGTTGCCAGCCAGCCC‑TGGCGACCATAGACGCTAAGA‑ATGCCGAAAACAGCAACCAGCACCGGGAGGATTAAGGTCATTGCGCACAGACGCAACAGCGCCAGCCGACCCGGAAAGCGCCTGCGATAGAGCGCGCGGGCGAGGAATATCGCGGGTACGACAGAGAGCAGCGCCGAGAGAAACGCCTGCCAGAAGga                                                                                                                                                                                                                 <  1:398633/251‑1 (MQ=255)
                                      cTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGTGTTGCCAGCCAGCCC‑TGGCGACCATAGACGCTAAGA‑ATGCCGAAAACAGCAACCAGCACCGGGAGGATTAAGGTCATTGCGCACAGACGCAACAGCGCCAGCCGACCCGGAAAGCGCCTGCGATAGAGCGCGCGGGCGAGGAATATCGCGGGTACGACAGAGACAAGCGCCGAGAGAAACGCCTGCCAGAAGgaga                                                                                                                                                                                                               >  2:539347/1‑249 (MQ=255)
                                       ttGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGTGTTGCCAGCCAGCCC‑TGGCGACCATAGACGCTAAGA‑ATGCCGAAAACAGCAACCAGCACCGGGAGGATTAAGGTCATTGCGCACAGACGCAACAGCGCCAGCCGACCCGGAAAGCGCCTGCGATAGAGCGCGCGGGCGGGGAATATCGCGGGTACGACAGAGAGCAGCGCCGAGAGAAAAGCCTGCCAGAAgacgaagc                                                                                                                                                                                                            >  1:26784/1‑244 (MQ=255)
                                        tGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGTGTTGCCAGCCAGCCC‑TGGCGACCATAGACGCTAAGA‑ATGCCGAAAACAGCAACCAGCACCGGGAGGATTAAGGTCATTGCGCACAGACGCAACAGCGCCAGCCGACCCGGAAAGCGCCTGCGATAGAGCGCGCGGGCGAGGAATATCGCGGGTACGACAGAGAGCAGCGCCGAGAGAAACGCCTGCCAGAAGGAGAAgc                                                                                                                                                                                                            <  1:120552/250‑1 (MQ=255)
                                        tGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGTGTTGCCAGCCAGCCC‑TGGCGACCATAGACGCTAAGA‑ATGCCGAAAACAGCAACCAGCACCGGGAGGATTAAGGTCATTGCGCACAGACGCAACAGCGCCAGCCGACCCGGAAAGCGCCTGCGATAGAGCGCGCGGGCGAGGAATATCGCGGGGACGACAGAGAGCAGCGCCGAAAGAAACACCTGCCAGAAGGAGAAgcg                                                                                                                                                                                                           >  2:641314/1‑251 (MQ=255)
                                           ggtccctacggCGAAAAGGTCTACTCCAGACCGAGCGTTTGGCAGAGTGTGACCAGCCACCCCGTGGC‑ACCATAATCGC‑CAGAGATGCCGAAAACAGCACCCAGCACCGGGAGGATTAAGGTCATTGGTGACAGACGCAACAGCGACAGCCGACCCGGAAAGCGCCTGCGATAGAGCGCGCGGGCGAAGAAGAGCGCGGGTACGACAGAGAGCAGCGCCGAGAGAAACGCCTGCCAGAAGGAGAAGCGcac                                                                                                                                                                                                        <  2:861808/242‑1 (MQ=255)
                                           aGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGTGTTGCCAGCCAGCCC‑TGGCGACCATAGACGCTAAGA‑ATGCCGAAAACAGCAACCAGCACCGGGAGGATTAAGGTCATTGCGCACAGACGCAACAGCGCCAGCCGACCCGGAAAGCGCCTGCGATAGAGCGCGCGGGCGAGGAATATCGCGGGTAAGACAGAGAGCAGCGCCGAGAGAAACGCCTGCCAGAAGGAGAAGCGcac                                                                                                                                                                                                        >  1:31148/1‑251 (MQ=255)
                                              ccGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGTGTTGCCAGCCAGCCC‑TGGCGACCATAGACGCTAAGA‑ATGCCGAAAACAGCAACCAGCACCGGGAGGATTAAGGTCATTGCGCACAGACGCAACAGCGCCAGCCGACCCGGAAAGCGCCTGCGATAGAGCGCGCGGGCGAGGAATATCGCGGGTACGACAGAGAGCAGCGCCGAGAGAAACGCCTGCCAGAAGGAGAAGCGcaccac                                                                                                                                                                                                     <  1:801013/251‑1 (MQ=255)
                                               cGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGTGTTGCCAGCCAGCCC‑TGGCGACCATAGACGCTAAGA‑ATGCCGAAAACAGCAACCAGCACCGGGAGGATTAAGGTCATTGCGCACAGACGCAACAGCGCCAGCCGACCCGGAAAGCGCCTGCGATAGAGCGCGCGGGCGAGGAATATCGCGGGAACGACAGAGAGCAGCGCCGAGAGAAACGCCAGCCAGAAGGAGAAGCGcaccacc                                                                                                                                                                                                    >  2:766666/1‑250 (MQ=255)
                                                 tAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGTGTTGCCAGCCAGCCC‑TGGCGACCATAGACGCTAAGA‑ATGCCGAAAACAGCAACCAGCACCGGGAGGATTAAGGTCATTGCGCACAGACGCAACAGCGCCAGCCGACCCGGAAAGCGCCTGCGATAGAGCGCGCGGGCGAGGAATATCGCGGGTACGACAGAGAGAAGCGGCGAGAGAAACGCCTGCCAGAAGGAGAAGCGCACCACATg                                                                                                                                                                                                  >  1:374082/1‑251 (MQ=255)
                                                    ggCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGTGTTGCCAGCCAGCCC‑TGGCGACCATAGACGCTAAGA‑ATGCCGAAAACAGCAACCAGCACCGGGAGGATTAAGGTCATTGCGCACAGACGCAACAGCGCCAGCCGACCCGGAAAGCGCCTGCGATAGAGCGCGCGGGCGAGGAATATCGCGGGTACGACAGAGAGCAGCGCCGAGAGAAACGCCTGCCAGAAGGAGAAGCGCACCACATGCca                                                                                                                                                                                               <  1:112226/251‑1 (MQ=255)
                                                       gAAAGGGTCCACTCCAGACCGAGCGATTGGCAGAGTGTTGCCAGCCAGCCC‑TGGCGACCATAGACGCTAAGA‑ATGCCGAAAACAGCAACCAGCACCGGGAGGATTAAGGTCATTGCGCACAGACGCAACAGCGCCAGCCGACCCGGAAAGCGCCTGCGATAGAGAGCGCGGGCGAGGAATATCGCGGGTACGACAGAGAGCAGAGCCGAGAGAAACGCCTGCCAGGAGGAGAAGCGCACCACATGCcac                                                                                                                                                                                              >  2:104050/1‑249 (MQ=255)
                                                                cACTCCAGACCGAGCGATTGGCAGAGTGTTGCAAGCCAGCCC‑TGGCGACCATAGACGCTAAGA‑ATGCCGAAAACAGCAACCAGCACCGGGAGGATTAAGGTCATTGCGCACAGACGCAACAGCGCCAGCCGACCCGGAAAGCGCCGGCGATAGAGCGCGCGGGCGAGGACTATCGCGGGTAAGACAGAGGGCAGCGCCGAGAGAAACGCCTGCCAGAAAGAGAAGGGCACCACATGCCAC‑AGATAGCTGtt                                                                                                                                                                                  >  2:953216/1‑250 (MQ=255)
                                                                cACTCCAGACAGGGCGTGTGGCAGAGGGTTGCCCGCCAGCCC‑TGGCGAACATAGACGCGAAGA‑ATGCTGAAAAAAGCAACCAGCACCGGGAGGATTAAGGTCATTGCGCTCAGACGCAACAGCGCCAGCCGACCCGGAAAGCGCCTGCGATAGAGCGCGCGGGCGAGGAATATCGCGGGTACGACAGAGAGCAGCGCCGAGAGAAACGCCTGCTAGAAGGAGAAGCGCACCACTTGCCAC‑AGATAGCTGTc                                                                                                                                                                                  <  2:617608/251‑1 (MQ=255)
                                                                 aCTCCAGACCGAGCGATTGGCAGAGTGTTGCCAGCCAGCCC‑TGGCGACCATAGACGCTAAGA‑ATGCCGAAAACAGCAACCAGCACCGGGAGGATTAAGGTCATTGCGCACAGACGCAACAGCGCCAGCCGACCCGGAAAGCGCCTGCGATAGAGCGCGCGGGCGAGGAATATCGCGGGTACGACAGAGAGCAGCGCCGAGAGAAACGCCTGCCAGAAGGAGAAGCGCACCACATGCCAC‑AGATAGCTGTcc                                                                                                                                                                                 >  1:85259/1‑251 (MQ=255)
                                                                          cGAGCGATTGGCAGAGTGTTGCCAGCCAGCCC‑TGGCGACCATAGACGCTAAGA‑ATGCCGAAAACAGCAACCAGCACCGGGAGGATTAAGGTCCTTGCGCACAGACGCAACAGCGCCAGCCGACCCGGAAAGCGCCTGCGATAGAGCGCGCGGGCGAGGAATATCGCGGGTACGACAGAGAGCAGCGCCGAGAGAAACGACTGACCGAAGGAGAAACGCACCACA‑ACCACCAGATAGCTGTCCTGcccagac                                                                                                                                                                        >  2:698433/1‑246 (MQ=255)
                                                                             gCGATTGGCAGAGTGTTGCCAGCCAGCCC‑TGGCGACCATAGACGCTAAGA‑ATGCCGAAAACAGCAACCAGCACCGGGAGGATTAAGGTCATTGCGCACAGACGCAACAGCGCCAGCCGACCCGGAAAGCGCCTGCGATAGAGCGCGCGGGCGAGGAATATCGCGGGTACGACAGAGAGCAGCGCCGAGAGAAACGCCTGCCAGAAGGAGAAGCGCACCACATGCAAC‑AGATAGCTGTCCTGCCAGACTGcc                                                                                                                                                                     <  1:993853/251‑1 (MQ=255)
                                                                              cGATTGGCAGAGTGTTGCCAGCCAGCCC‑TGGCGACCATAGACGCTAAGA‑ATGCCGAAAACAGCAACCAGCACCGGGAGGATTAAGGTCATTGCGCACAGACGCAACAGCGCCAGCCGACCCGGAAAGCGCCTGCGATAGAGCGCGCGGGCGAGGAATATCGCGGGTACGACAGAGAGCAGCGCCGAGAGAAACGCCTGCCAGAAGGAGAAGCGCACCACATGCCAC‑AGATAGCTGTCCTGCCAGACTGCCa                                                                                                                                                                    <  1:421600/251‑1 (MQ=255)
                                                                                   ggCAGAGTGTTGCCAGCAAGCCC‑TGGCGACCATAGACGCTAAGA‑ATGCCGAAAACAGCAACCAGCACCGGGAGGATTAAGGTCATTGCGCACAGACGCAACAGCGCCAGCCGACCCGGAAAGCGCCTGCGATAGAGCGCGCGGGCGAGGAATATCGCGGGTACGACAGAGAGCAGCGCCGAGAGAAACGCCTGCCAGAAGGAGAAGCGCACCACATGCCAC‑AGATAGCTGTCCTGCCAGACTGCCACCCAg                                                                                                                                                               <  2:655436/251‑1 (MQ=255)
                                                                                      agagTGTTGCCAGCCAGCCC‑TGGCGACCATAGACGCTAAGA‑ATGCCGAAAACAGCAACCAGCACCGGGAGGATTAAGGTCATTGCGCACAGACGCAACAGCGCCAGCCGACCCGGAAAGCGCCTGCGATAGAGCGCGCGGGCGAGGAATATCGCGGGTACGACAGAGAGCAGCGCCGAGAGAAACGCCTGCCAGAAGGAGAAGCGCACCACATGCCAC‑AGATAGCTGTCCTGCCAGACTGCCACCCAGTCa                                                                                                                                                            <  1:104050/251‑1 (MQ=255)
                                                                                                 agccagccC‑TGGCGACCATAGACGCTAAGA‑ATGCCGAAAACAGCAACCAGCACCGGGAGGATTAAGGTCATTGCGCACAGACGCAACAGCGCCAGCCGACCCGGAAAGCGCCTGCGATAGAGCGCGCGGGCGAGGAATATCGCGGGTACGACAGAGAGCAGCGCCGAGAGAAACGCCTGCCAGAAGGAGAAGCGCACCACATGCCAC‑AGATAGCTGTCCTGCCAGACTGCCACCCAGTCATCCTGCGgcgc                                                                                                                                                 >  1:935404/1‑251 (MQ=255)
                                                                                                  gccagccC‑TGTGGACCATAGACGTTAAGA‑ATGCCGAAAACGGCACCCATCCCCGGGTGGACTAAGGTCAGTGCGAACAGAAGCAACAGCGTCAGCCGAACCGGAAATCGCCTGCGAGAGAGCGCGCGGGCGATGAAAATCGCGGGTCCGACAGAGAGCAGTGCCGAGAGAAACGCCTGCCAGAAGGAGAAGCTCACCGCATGCCCC‑AGATAGCTGTCCTTTCAGACTGCCACCCAGTCATCCTGCGGcgcg                                                                                                                                                <  2:178774/251‑1 (MQ=255)
                                                                                                   ccagccC‑TGGCGACCATAGACGCTAAGA‑ATGCCGAAAACAGCAACCAGCACCGGGAGGATTAAGGTCATTGCGCACAGACGCAACAGCGCCAGCCGACCCGGAAAGCGCCTGCGATAGAGCGCGCGGGCGAGGAATATCGCGGGTACGACAGAGAGCAGCGCCGAGAGAAACGCCTGCCAGAAGGAGAAGCGCACCACATGCCAC‑AGATAGCTGTCCTGCCAGACTGCCACCCAGTCATCCTGCGGCGCGt                                                                                                                                               >  1:715070/1‑251 (MQ=255)
                                                                                                   ccagccC‑TGGCGACCATAGACGCTAAGA‑ATGCCGAAAACAGCAACCAGCACCGGGAGGATTAAGGTCATTGCGCACAGACGCAACAGCGCCAGCCGACCCGGAAAGCGCCTGCGATAGAGCGCGCGGGCGAGGAATATCGCGGGTACGACAGAGAGCAGCGCCGAGAGAAACGCCTGCCAGAAGGAGAAGCGCACCACATGCCAC‑AGATAGCTGTCCTGCCAGACTGCCACCCAGTCATCCTGCGGCGCGt                                                                                                                                               >  1:359843/1‑251 (MQ=255)
                                                                                                    cagccC‑TGGCGACCATAGACGCTAAGA‑ATGCCGAAAACAGCAACCAGCACCGGGAGGATTAAGGTCATTGCGCACAGACGCAACAGCGCCAGCCGACCCGGAAAGCGCCTGCGATAGAGCGCGCGGGCGAGGAATATCGCGGGTACGACAGAGAGCAGCGCCGAGAGAAACGCCTGCCAGAAGGAGAAGCGCACCACATGCCAC‑AGATAGCTGTCCTGCCAGACTGCCACCCAGTCATCCTGCGGCGCGtt                                                                                                                                              <  1:302259/251‑1 (MQ=255)
                                                                                                       ccc‑TGGCGACCATAGACGCTAAGA‑ATGCCGAAAACAGCAACCAGCACCGGGAGGATTAAGGTCATTGCGCACAGACGCAACAGCGCCAGCCGACCCGGAAAGCGCCTGCGATAGAGCGCGCGGGCGAGGAATATCGCGGGTACGACAGAGAGCAGCGCCGAGAGAAACGCCTGCCAGAAGGAGAAGCGCACCACATGCCAC‑AGATAGCTGTCCTGCCAGACTGCCACCCAGTCATCCTGCGGCGCGTTccc                                                                                                                                           >  1:783684/1‑250 (MQ=255)
                                                                                                                    tAGACGCTAAGA‑ATGCCGAAAACAGCAACCAGCACCGGGAGGATTAAGGTCATTGCGCACAGACGCAACAGCGCCAGCCGACCCGGAAAGCGCCTGCGATAGAGCGCGCGGGCGAGGAATATCGCGGGTACGACAGAGAGCAGCGCCGAGCGAAACGCCTGCCAGAAGGAGAAGCGCACCACATGCCAC‑AGATAGCTGTCCTGCCAGCCTGCCACCCAGTCATCCTGCGGCGCGTTCcaccaccggcag                                                                                                                                 >  2:172331/1‑243 (MQ=255)
                                                                                                                      gACGCTAAGA‑ATGCCGAAAACAGCAACCAGCACCGGGAGGATTAAGGTCATTGCGCACAGACGCAACAGCGCCAGCCGACCCGGAAAGCGCCTGCGATAGAGCGCGCGGGCGAGGAATATCGCGGGTACGACAGAGAGCAGCGCCGAGAGAAACGCCTGCCAGAAGGAGAAGCGCACCACATGCCAC‑AGATAGCTGTCCTGCCAGACTGCCACCCAGTCATCCTGCGGCGCGTTCCACCACAGGGCGAGaa                                                                                                                             <  1:410616/251‑1 (MQ=255)
                                                                                                                       aCGCTAAGA‑ATGCCGAAAACAGCAACCAGCACCGGGAGGATTAAGGTCATTGCGCACAGACGCAACAGCGCCAGCCGACCCGGAAAGCGCCTGCGATAGAGCGCGCGGGCGAGGAATATCGCGGGTACGACAGAGAGCAGCGCCGAGAGAAACGCCTGCCAGAAGGAGAAGCGCACCACATGCCAC‑AGATAGCTGTCCTGCCAGACTGCCACCCAGTCATCCTGCGGCGCGTTCCACCACAGGGCGAGaa                                                                                                                             >  1:86302/1‑250 (MQ=255)
                                                                                                                         gCTAAGA‑ATGCCGAAAACAGCAACCAGCACCGGGAGGATTAAGGTCATTGCGCACAGACGCAACAGCGCCAGCCGACCCGGAAAGCGCCTGCGATAGAGCGCGCGGGCGAGGAATATCGCGGGTACGACAGAGAGCAGCGCCGAGAGAAACGCCTGCCAGAAGGAGAAGCGCACCACATGCCAC‑AGATAGCTGTCCTGCCAGACTGCCACCCAGTCATCCTGCGGCGCGTTCCACCACAGGGCGAGAAAcg                                                                                                                          >  1:891739/1‑251 (MQ=255)
                                                                                                                         gCTAAGA‑AGGCCGAAAACAGCGACCAACACCGGGAGGATCAAGGTCATTGCACACAGACGCAACAGCGCCAGCCGACCCGGAAAGCGCCTGCGATAGAGCGCGCGGGCGAGGAATATCGCGGGTATGACAGAGAGCAGTGCCGAGAGAAACGCCTGCCAGAAGGAGAAGCGCACCACATGC‑AC‑AGATAGCTGTCCTGCCAGACTGCCACCCAGTCATCCTGCGGCGCGTTCCACCACAGGGCGAGAAAcgc                                                                                                                         <  1:42384/251‑1 (MQ=255)
                                                                                                                            aaGA‑ATGCCGAAAACAGCAACCAGCACCGGGAGGATTAAGGTCATTGCGCACAGACGCAACAGCGCCAGCCGACCCGGAAAGCGCCTGCGATAGAGCGCGCGGGCGAGGAATATCGCGGGTACGACAGAGAGCAGCGCCGAGAGAAACGCCTGCCAGAAGGAGAAGCGCACCACATGCCAC‑AGATAGCTGTCCTGCCAGACTGCCACCCAGTCATCCTGCGGCGCGTTCCACCACAGGGCGAGAAAcgccg                                                                                                                       >  2:775333/1‑251 (MQ=255)
                                                                                                                              gA‑ATGCCGAAAACAGCAACCAGCACCGGGAGGATTAAGGTCATTGCGCACAGACGCAACAGCGCCAGCCGACCCGGAAAGCGCCTGCGATAGAGCGCGCGGGCGAGGAATATCGCGGGTACGACAGAGAGCAGCGCCGAGAGAAACGCCTGCCAGAAGGAGAAGCGCACCACATGCCAC‑AGATAGCTGTCCTGCCAGACTGCCACCCAGTCATCCTGCGGCGCGTTCCACCACAGGGCGAGACACgccgcc                                                                                                                     >  2:299057/1‑251 (MQ=255)
                                                                                                                              gA‑ATGCCGAAAACAGCAACCAGCACCGGGAGGATTAAGGTCATTGCGCACAGACGCAACAGCGCCAGCCGACCCGGAAAGCGCCTGCGATAGAGCGCGCGGGCGAGGAATATCGCGGGTACGACAGAGAGCAGCGCCGAGAGAAACGCCTGCCAGAAGGAGAAGCGCACCACATGCCAC‑AGATAGCTGTCCTGCCAGACTGCCACCCAGTCATCCTGCGGCGCGTTCCACCACAGGGCGAGAAACgccgcc                                                                                                                     <  1:116783/251‑1 (MQ=255)
                                                                                                                                   gCCGAAAACAGCAACCAGCACCGGGAGGATTAAGGTCATTGCGCACAGACGCAACAGCGCCAGCCGACCCGGAAAGCGCCTGCGATAGAGCGCGCGGGCGAGGAATATCGCGGGTACGACAGAGAGCAGCGCCGAGAGAAACGCCTGCCAGAAGGAGAAGCGCACCACATGCCAC‑AGATAGCTGTCCTGCCAGACTGCCACCCAGTCATCCTGCGGCGCGTTCCACCACAGGGCGAGAAACGCCGCCAgcg                                                                                                                 <  1:283797/251‑1 (MQ=255)
                                                                                                                                        aaaCAGCAACCAGCACCGGGAGGATTAAGGTCATTGCGCACAGACGCAACAGCGCCAGCCGACCCGGAAAGCGCCTGCGATAGAGCGCGCGGGCGAGGAATATCGCGGGTACGACAGAGAGCAGCGCCGAGAGAAACGCCTGCCAGAAGGAGAAGCGCACCACATGCCAC‑AGATAGCTGTCCTGCCAGACTGCCACCCAGTCATCCTGCGGCGCGTTCCACCACAGGGCGAGAAACGCCGCCAGCGCAAcc                                                                                                            <  1:187229/251‑1 (MQ=255)
                                                                                                                                                        cGGGAGGATTAAGGTCATTGCGCACAGACGCAACAGCGCCAGCCGACCCGGAAAGCGCCTGCGATAGAGCGCGCGGGCGAGGAATATCGCGGGTACGACAGAGAGCAGCGCCGAGAGAAACGCCTGCCAGAAGGAGAAGCGCACCACATGCCAC‑AGATAGCTGTCCTGCCAGACTGCCACCCAGTCATCCTGCGGCGCGTTCCACCACAGGGCGAGAAACGCCGCCAGCGCAACCGCTACCACCAGCgtgg                                                                                            <  1:160580/251‑1 (MQ=255)
                                                                                                                                                         gggAGGATTAAGGTCATTGCGCACAGACGCAACAGCGCCAGCCGACCCGGAAAGCGCCTGCGATAGAGCGCGCGGGCGAGGAATATCGCGGGTACGACAGAGAGCAGCGCCGAGAGAAACGCCTGCCAGAAGGAGAAGCGCACCACATGCCAC‑AGATAGCTGTCCTGCCAGACTGCCACCCAGTc                                                                                                                                                             <  1:524316/185‑1 (MQ=255)
                                                                                                                                                                  aaggtcagagcccaaaGACGAAACAGCGCCAGCCGACCCGGAAAGCGCCTGCGATAGAGCGCGCGGGCGAGGAATATCGCGGGTACGACAGAGAGCAGCGCCGAGAGAAACGCCTGCCAGAAGGAGAAGCTCACCACATGCCAC‑AGATAGCTGTCCTGCCAGACTGCCACCCAGTCATCCTGCGGCGCGTTCCACCACAGGGCGAGAAACGCCGCCAGCGCAACCGCTACCACCAGCGTGGTGGCGCTTa                                                                                   <  2:125653/235‑1 (MQ=255)
                                                                                                                                                                  aagggcaatgcgcacaGACGCAACAGCGCCAGCCGACCCGGAAAGCGCCTGCGATAGAGCGCGCGGGCGAGGAATATCGCGGGTACGACAGAGAGCAGCGCCGAGAGAAACGCCTGCCAGAAGGAGAAGCGCACCACATGCCAC‑AGATAGCTGTCCTGCCAGACTGCCACCCAGTCATCCTGCGGCGCGTTCCACCACAGGGCGAGAAACGCCGCCAGCGCAACCGCTACCACCAGCGTGGTGGCGCTTa                                                                                   <  2:841638/238‑1 (MQ=255)
                                                                                                                                                                  aaGGTCATTGCGCACGGACGCAACACGGCCAGCCGACCCGGAAAGCGCCTGCGATAGAGCGCGCGGGCGAGGAATATCGCGGGTACGACAGAGAGCAGCGCCGAGAGAAACGCCTGCCAGAAGGAGAAGCGCACCACATGCCAC‑AGATAGCTGTCCTGCCAGACTGCCACCCAGTCATCCTGCGGCGCGTTCCACCACAGGGCGAGAAACGCCGCCAGCGCAACCGCTACCACCAGCGTGGTGGCGCTTac                                                                                  <  2:832957/251‑1 (MQ=255)
                                                                                                                                                                   agggcATTGCGCACAGACGCAACAGCGCCAGCCGACCCGGAAAGCGCCTGCGATAGAGCGCGCGGGCGAGGAATATCGCGGGTACGACAGAGAGCAGCGCCGAGAGAAACGCCTGCCAGAAGGAGAAGCGCACCACATGCCAC‑AGATAGCTGTCCTGCCAGACTGCCACCCAGTCATCCTGCGGCGCGTTCCACCACAGGGCGAGAAACGCCGCCAGCGCAACCGCTACCACCAGCGTGGTGGCGCTTaca                                                                                 <  1:318873/247‑1 (MQ=255)
                                                                                                                                                                     gTCATTGCGCACAGACGCAACAGCGCCAGCCGACCCGGAAAGCGCCTGCGATAGAGCGCGCGGGCGAGGAATATCGCGGGTACGACAGAGAGCAGCGCCGAGAGAAACGCCTGCCAGAAGGAGAAGCGCACCACATGCCAC‑AGATAGCTGTCCTGCCAGACTGCCACCCAGTCATCCTGCGGCGCGTTCCACCACAGGGCGAGAAACGCCGCCAGCGCAACCGCTACCACCAGCGTGGTGGCGCTTACAcc                                                                               <  1:698256/251‑1 (MQ=255)
                                                                                                                                                                        attgcgcccaGACGCAACAGCGCCAGCCGATCCGGAAAGCGCCTGCGATAGAGCGCGCGGGCGAGGAATATCGCGGGTACGACAGAGAGCAGCGCCGAGAGAAACGCCTGCCAGAAGGAGAAGCGCACCACATGCCAC‑AGATAGCTGTCCTGCCAGACTGCCCCCCAGTCATCCTGCGGCGCGTTCCACCACAGGTCGAGAAACGCCGCCAGCGCAACCGCTACCACCAGCGTGGTGGCGCTTACACCTgg                                                                            <  2:104803/243‑1 (MQ=255)
                                                                                                                                                                           gcgcacaGACGCAACAGCGCCAGCCGACCCGGAAAGCGCCTGCGATAGAGCGCGCGGGCGAGGAATATCGCGGGTACGACAGAGAGCAGCGCCGAGAGAAACGCCTGCCAGAAGGAGAAGCGCACCACATGCCAC‑AGATAGCTGTCCTGCCAGACTGCCACCCAGTCATCCTGCGGCGCGTTCCACCACAGGGCGAGAAACGCCGCCAGCGCAACCGCTACCACCAGCGTGGTGGCGCTTACACCTGGAAt                                                                         <  1:827677/248‑1 (MQ=255)
                                                                                                                                                                              cacaGACGCAACAGCGCCAGCCGACCCGGAAAGCGCCTGCGATAGAGCGCGCGGGCGAGGAATATCGCGGGTACGACAGAGAGCAGCGCCGAGAGAAACGCCTGCCAGAAGGAGAAGCGCACCACATGCCAC‑AGATAGCTGTCCTGCCAGACTGCCACCCAGTCATCCTGCGGCGCGTTCCACCACAGGGCGAGAAACGCCGCCAGCGCAACCGCTACCACCAGCGTGGTGGCGCTTACACCTGGAATTa                                                                       >  1:169488/1‑250 (MQ=255)
                                                                                                                                                                                   ccgcaatcgcgcCATCCGTCACGGAAAGCGCCTGCGATAGAGCGCGCGGGCTAGTAATATCGCGGGTACCACAGAGAGCAGCGCCGAGAGAAACGCCTGCCAGAAGGAGAAGCGCACCGCATGCCAT‑AGATAGCTGTCCTGCCAGACTGCCACCCAGGCATCCTGCGGCTCGTTCCACCACAGGGCGAGAAACGCCGCCAGCGCAACCGCTACCACCAGCTTGGTGGCGCTTACACCTGGAATTAACCAGc                                                                 <  2:439899/243‑1 (MQ=255)
                                                                                                                                                                                   aCGCAACAGCGCCAGCCGACCCGGAAAGCGCCTGCGATAGAGCGCGCGGGCGAGGAATATCGCGGGTACGACAGAGAGCAGCGCCGAGAGAAACGCCTGCCAGAAGGAGAAGCGCACCACATGCCAC‑AGATAGCTGTCCTGCCAGACTGCCACCCAGTCATCCTGCGGCGCGTTCCACCACAGGGCGAGAAACGCCGCCAGCGCAACCGCTACCACCAGCGTGGTGGCGCTTACACCTGGAATTAACCAGc                                                                 <  1:825769/251‑1 (MQ=255)
                                                                                                                                                                                    cGCAACATCGCCAGCCGACACGGAAAGCGCCGGCGATAGAGCGCGCGGGGGCGGACTATCGCGGGTACCACAGAGAGCAGCGCCGCGCGAAACGCCTGCCAGAAGGAGAAGCGCACCACATGCCAA‑CGCTAGCTGTCCTGCCAGACTGCCAACCAGTCATCCTGCGGCGCGTTCCACCACAGGGCGAGCAACGCCGCCAGCGCAACCGCTACCACCAGCGTGGTGGCGCTTACCACTGGAATTAACCAGcc                                                                >  2:480706/1‑251 (MQ=255)
                                                                                                                                                                                      cAACAGCGCCAGCCGACCCGGAAAGCGCCTGCGATAGAGCGCGCGGGCGAGGAATATCGCGGGTACGACAGAGAGCAGCGCCGAGAGAAACGCCTGCCAGAAGGAGAAGCGCACCACATGCCAC‑AGATAGCTGTCCTGCCAGACTGCCACCCAGTCATCCTGCGGCGCGTTCCACCACAGGGCGAGAAACGCCGCCAGCGCAACCGCTACCACCAGCGTGGTGGCGCTTACACCTGGAATTAACCAGCCgg                                                              <  1:711373/251‑1 (MQ=255)
                                                                                                                                                                                      cAACAGCGCCAGCCGACCCGGAAAGCGCCTGCGAGAGAGCGCGCGGGCGAGGAATATCGCGGGTACGACAGAGAGCAGCGCCGAGAGAAACGCCTGCCAGAAGGAGAAGCGCACCACATGCCAC‑AGATAGCTGTCCTGCCAGACTGCCACCCAGTCGTCCTGCGGCGCGTTCCACCACAGGGCGAGAAACGCCGCCAGCGCAACCGCTGCCACCAGCGTGGTGGCGCTTACACCTGGAATTAACCAGCCgg                                                              <  2:455465/251‑1 (MQ=255)
                                                                                                                                                                                       aaCAGCGCCAGCCGACCCGGAAAGCGCCTGCGATAGAGCGCGCGGGCGAGGAATATCGCGGGTACGACAGAGAGCAGCGCCGAGAGAAACGCCTGCCAGAAGGAGAAGCGCACCACATGCCAC‑AGATAGCTGTCCTGCCAGACTGCCACCCAGTCATCCTGCGGCGCGTTCCACCACAGGGCGAGAAACGCCGCCAGCGCAACCGCTACCACCAGCGTGGTGGCGCTTCCACCTGGAATTAACCAGCCggg                                                             >  1:807234/1‑251 (MQ=255)
                                                                                                                                                                                       aaCAGCGCCAGCCGACCCGGAAAGCGCCTGCGATAGAGCGCGCGGGCGAGGAATATCGCGGGTACGACAGAGAGCAGCGCCGAGAGAAACGCCTGCCAGAAGGAGAAGCGCACCACATGCCAC‑AGATAGCTGTCCTGCCAGACTGCCACCCAGTCATCCTGCGGCGCGTTCCACCACAGGGCGAGAAACGCCGCCAGCGCAACCGCTACCACCAGCGTGGTGGCGCTTACACCTGGAATTAACCAGCCggg                                                             <  1:40951/251‑1 (MQ=255)
                                                                                                                                                                                         cgtggcCAGACGACCCGGAAAGCGCCGGGGATAGAGCGCGCGGGCGAGGAATATCGCGGGTACGACAGAGAGCAGCGCCGAGAGAAACGCCGGCCAGAAGGAGAAGCGCACCACATGCCAC‑AGATAGCTGTCCTGCCAGACTGCCACCCAGTCATCCTGCGGCGCGTTCCACCACAGGGCGAGAAACGCCGCCAGCGCAACCGCTACCACCAGCGTGGTGGCGCTTACACCTGGAATTAACCAGCCGGGaa                                                           <  2:957837/247‑1 (MQ=255)
                                                                                                                                                                                         catcgcCAGCCGACCCGGAAAGCGCCTGCGATAGAGCGCGCGGGCGAGGAATATCGCGGGTACGACAGAGAGCAGCGCCGAGAGAAACGCCTGCCAGAAGGAGAAGCGCACCACATGCCAC‑AGATAGCTGTCCTGCCAGACTGCCACCCAGTCATCCTGCGGCGCGTTCCACCACCGGGCGAGAAACGCCGCCAGCGCAACCGCTACCAACAGCGTGGTGGCGCTTACACCTGGAATTAACCAGCTgggt                                                            >  2:274691/4‑249 (MQ=255)
                                                                                                                                                                                             gcCAGCCGACCCGGAAAGCGCCTGCGATAGAGCGCGCGGGCGAGGAATATCGCGGGTACGACAGAGAGCAGCGCCGAGAGAAACGCCTGCCAGAAGGAGAAGCGCACCACATGCCAC‑AGATAGCTGTCCTGCCAGACTGCCACCCAGTCATCCTGCGGCGCGTTCCACCACAGGGCGAGAAACGCCGCCAGCGCAACCGCTACCACCAGCGTGGTGGCGCTTACACCTGGAATTAACCAGCCGGGACTTa                                                        >  1:100088/1‑250 (MQ=255)
                                                                                                                                                                                             gcCAGCCGAACCGGAAAGCGCCTGCGATAGCGCGCGCGGGCGAGGAATATCGCGGGTACGACAGAGAACAGCGCCGAGAGAAACGCCTGCCCGAAGGAGAAGCGCACCACATGCCAC‑AGATAGCTGTCCTACCAGACTGCCACCCAGTCATCCCGCGGCGCGTTCCCCCAACGGGCGAGAAACGCCGCCAGCGCACCCGCTCCCACCCGCGCGGTGGCGCTTACACATGGAATTACCCAACCggtcattc                                                        >  2:128726/1‑244 (MQ=255)
                                                                                                                                                                                                aGCCGACCCGGAAAGCGCCTGCGATAGAGCGCGCGGGCGAGGAATATCGCGGGTACGACAGAGAGCAGCGCCGAGAGAAACGCCTGCCAGAAGGAGAAGCGCACCACATGCCAC‑AGATAGCTGTCCTGCCAGACTGCCACCCAGTCATCCTGCGGCGCGTTCCACCACAGGGCGAGAAACGCCGCCAGCGCAACCGCTACCACCAGCGTGGTGGCGCTTACACCTGGAATTAACCAGCCGGGAATTAACgg                                                    >  1:585971/1‑251 (MQ=255)
                                                                                                                                                                                                 gccacgcgggaaagagctagCGATAGAGCGCGAGGGCGGGGAATATCGCGGGTCCGACAGAGAGCAGCGCAGAGAGAAACGCCTGCCAGAAGGAGAATCTCACCACATGCCAC‑AGATATCTTTCCTGCCAGACTGCCACCCAGTCCTCCTGCGGCGCGTTCCACCACAGGGCGAGAAACGCTTCTAGCGCAACCGCGACCACCAGCGTGGTGGCGCTTACACCTGTAATTAACCAGCCGTGAATTaagggt                                                   <  2:273539/232‑5 (MQ=255)
                                                                                                                                                                                                   cGACCCGGAAAGCGCCTGCGATAGAGCGCGCGGGCGAGGAATATCGCGGGTACGACAGAGAGCAGCGCCGAGAGAAACGCCTGCCAGAAGGAGAAGCGCACCACATGCCAC‑AGATAGCTGTCCTGCCAGACTGCCACCCAGTCATCCTGCGGCGCGTTCCACCACAGGGCGAGAAACGCCGCCAGCGCAACCGCTACCACCAGCGTGGTGGCGCTTACACCTGGAATTAACCAGCCGGGAATTAACGGCTg                                                 <  1:525997/251‑1 (MQ=255)
                                                                                                                                                                                                    gACCCGGAAAGCGCCTGCGATAGAGCGCGCGGGCGAGGAATATCGCGGGTACGACAGAGAGCAGCGCCGAGAGAAACGCCTGCCAGAAGGAGAAGCGCACCACATGCCAC‑AGATAGCTGTCCTGCCAGACTGCCACCCAGTCATCCTGCGGCGCGTTCCACCACAGGGCGAGAAACGCCGCCAGCGCAACCGCTACCACCAGCGTGGTGGCGCTTACACCTGGAATTAACCAGCCGGGAATTAACGGCTg                                                 >  1:807052/1‑250 (MQ=255)
                                                                                                                                                                                                      cccGGAAAGCGCCTGCGATAGAGCGCGCGGGCGAGGAATATCGCGGGTACGACAGAGAGCAGCGCCGAGAGAAACGCCTGCCAGAAGGAGAAGCGCACCACATGCCAC‑AGATAGCTGTCCTGCCAGACTGCCACCCAGTCATCCTGCGGCGCGTTCCACCACAGGGCGAGAAACGCCGCTAGCGCAACCGCTACCACCAGCGTGGTGGCGCTTACACCTGGACTTAACCAGCCGGGAATTAACGGCTgg                                                >  2:412188/1‑248 (MQ=255)
                                                                                                                                                                                                      cccGGAAAGCGCCGGCGATAGAGCGCGCGGGCGAGGAATTTCGCGGGTACGACAGAGAGCAGCGCCGAGAGAACCGCCTGCCAGAAGGAGAAGCGCACCACATGCCAC‑AGAGAGCTGTCCTGCCAGACTGCCACCCAGTCATCCTGCGGCGCGTTCCACCACAGGGCGAGAAACGCCGCCAGCGCAACCGCTACCACCAGCGTGGTGGCGCGTACACCTGGAATTAACCAGCCGGGAATTAACGGCTGACg                                              <  2:647700/251‑1 (MQ=255)
                                                                                                                                                                                                           aaaGCGGCTGCGATAGAGCGCGCGGGCGAGGAATATCGCGGGTACGACAGAGAGCAGCGCCGAGAGAAACGCCTGCCAGAAGGAGAAGCGCACCACATGCCAC‑AGATAGCTGTCCTGCCAGACTGCCACCCAGTCATACTGCGGCGCGTTCCACCACAGGGCGAGAAACGCCGCCAGCGCAACCGCTACCACCATCGTGGTGGCGCTTACACCTGGAATTAACCAGCCGGGAATTAACGGCTGACAGcgcg                                         >  2:930595/1‑251 (MQ=255)
                                                                                                                                                                                                           aaaGCGCCTGCGATAGAGCGCGCGGGCGAGGAATATCGCGGGTACGACAGAGAGCAGCGCCGAGAGAAACGCCTGCCAGAAGGAGAAGCGCACCACATGCCAC‑AGATAGCTGTCCTGCCAGACTGCCACCCAGTCATCCTGCGGCGCGTTCCACCACAGGGCGAGAAACGCCGCCAGCGCAACCGCTACCACCAGCGTGGTGGCGCTTACACCTGGAATTAACCAGCCGGGAATTAACGGCTGACGGcgcg                                         >  1:742303/1‑251 (MQ=255)
                                                                                                                                                                                                           aaaGCGCCTGCGAGAGAGCGCGCGGGCGAGGAATATCGCGGGTACGACAGAGAGCAGCGCCGAGAGAAACGCCTGCCAGAAGGAGAAGCGCACCACATGCCAC‑AGATAGCTGTCCTGCCAGACTGCCACCCAGTCATCCTGCGGCGCGTTCCACCACAGGGCGAGAAACGCCGCCAGCGCAACCGCTACCACCAGCGTGGTGGCGCTTACACCTGGAATTAACCAGCCGGGAATTAACGGCTGACGGcgcg                                         <  1:694755/251‑1 (MQ=255)
                                                                                                                                                                                                                  gtGCGATAGAGCGCGCGGGGGAGGAATACATCGGGTACGAACGAGAGCTGCGCCGAGAGTAACGCCCGCCAGAATGAGAAGCGTACCACATTCCAC‑AGATAGCTGTCCTGCCAGCCGGCCACCCAGTCATCCTGCGGCGCGTTCCACCACAGGGCGAGAAACGCCGCCAGCGCAACCGCTACCACCAGCGTGGTGGCGCTTACATCTGGAATTAACCAGCCGGGAATTAACGGCTGACGGCGCGTTGCCAt                                  <  2:766292/250‑1 (MQ=255)
                                                                                                                                                                                                                        tAGAGCGCGCGGGCGAGGAATATCGCGGGTACGACAGAGAGCAGCGCCGAGAGAAACGCCTGCCAGAAGGAGAAGCGCACCACATGCCAC‑AGATAGCTGTCCTGCCAGACTGCCACCCAGTCATCCTGCGGCGCGTTCCACCACAGGGCGAGAAACGCCGCCAGCGCAACCGCTACCACCAGCGTGGTGGCGCTTACACCTGGAATTAACCAGCCGGGAATTAACGGCTGACGGCGCGTTGCCATTCGCt                             >  1:762073/1‑250 (MQ=255)
                                                                                                                                                                                                                         agagCGCGCGGGCGAGGAATATCGCGGGTACGACAGAGAGCAGCGCCGAGAGAAACGCCTGCCAGAAGGAGAAGCGCACCACATGCCAC‑AGATAGCTGTCCTGCCAGACTGCCACCCAGTCATCCTGCGGCGCGTTCCACCACAGGGCGAGAAACGCCGCCAGCGCAACCGCTACCACCAGCGTGGTGGCGCTTACACCTGGAATTAACCAGCCGGGAATTAACGGCTGACGGCGCGTTGCCATTCGCTaa                           <  1:471497/251‑1 (MQ=255)
                                                                                                                                                                                                                           agCGCGCGGGCGAGGAATATCGCGGGTACGACAGAGAGCAGCGCCGAGAGAAACGCCTGCAAGAAGGAGAAGCGCACCACATGCCAC‑AGATAGCTGTCCTGCCAGACTGCCACCCAGTCATCCTGCGGCGCGTTCCACCACAGGGCGAGAAACGCCGCCAGCGCAACCGCTACCACCAGCGTGGTGGCGCTTACACCTGGAATTAACCAGCCGGGAATTAACGGCTGACGGCGCGTTGCCCTTCGCTAATc                         >  1:62492/1‑251 (MQ=255)
                                                                                                                                                                                                                           agCGCGCGGGCGAGGAATATAGCGGGTACGACAGAGAGCAGCGCCGAGAGAAACGCCTGCCAGAAGGAGAAGCGCACCACATGCCAC‑AGATAGCTGTCCTGCCAGACTGCCACCCAGTCATACTGCGGCGCGTTCCACCACAGGGCGAGAAACGCCGCCAGCGCAACCGCTACCACCAGCGTGGTGGCGCTTACACCTGGAATTAACCAGCCGGGAATTAACGGCTGACGGCGCGTTGCCATTCGCTAATc                         >  1:764794/1‑251 (MQ=255)
                                                                                                                                                                                                                             cgcgcgGGCGAGGAATATCGCGGGTACGACAGAGAGCAGCGCCGAGAGAAACGCCTGCCAGAAGGAGAAGCGCACCACATGCCAC‑AGATAGCTGTCCTGCCAGACTGCCACCCAGTCATCCTGCGGCGCGTTCCACCACAGGGCGAGAAACGCCGCCAGCGCAACCGCTACCACCAGCGTGGTGGCGCTTACACCTGGAATTAACCAGCCGGGAATTAACGGCTGACGGCGCGTTGCCATTCGCTAATCCa                       <  1:536104/251‑1 (MQ=255)
                                                                                                                                                                                                                               cgcgGGCGAGGAATATCGCGGGTACGACAGAGAGCAGCGCCGAGAGAAACGCCTGCCAGAAGGAGAAGCGCACCACATGCCAC‑AGATAGCTGTCCTGCCAGACTGCCACCCAGTCATCCTGCGGCGCGTTCCACCACAGGGCGAGAAACGCCGCCAGCGCAACCGCTACCACCAGCGTGGTGGCGCTTACACCTGGAATTAACCAGCCGGGAATTAACGGCTGACGGCGCGTTGCCATTCGCTAATCCATg                     >  1:683852/1‑251 (MQ=255)
                                                                                                                                                                                                                                    gCGAGGAATATCGCGGGTACGACAGAGAGCAGCGCCGAGAGCAACGCCTGCCAGAAGGAGAAGCGCACCACATGCCAC‑AGATAGCTGTCCTGCCAGACTGCCACCCAGTCATCCTGCGGCGCGGTCCACCACAGGGCGAGAAACGCCGCCAGCGCAACCGCTACCACCAGCGCGGTGGCGCTTACACCTGGAATTAACCAGCCGGGAATTAACGGCTGACGGCGCGGTGCCATTCGCTAATCcctgcctgg                >  2:634508/1‑250 (MQ=255)
                                                                                                                                                                                                                                    gCGAGGAAGAGCGCGGGTACGACAGAGAGCAGCGCCGAGAGAAACGCATGCAAGAAGGAGAATCGCACCACAGGCCGA‑AGATAGCTGCCCTGCCATACTCCCACCCAGTCATCCTGCGGCGCGTTCCACCACAGGGCGATAAACGCCGCCAGCGCAACCGCTACCACCAGCGTGGTGGCGCTTACATCTGGAATTAACCAGCCGGGAATTAACGGCGGACGGCGCGTTGCCATTCGCTAATCCATGCCTGa                <  2:355785/251‑1 (MQ=255)
                                                                                                                                                                                                                                     cGAGGAATATCGCGGGTACGACAGAGAGCAGCGCCGCGAGAAACGCCTGCCAGAAGGAGAAGCGCACCACATGCCAC‑AGATAGCTGTCCTGCCAGACTGCCACCCAGTCATCCTGCGGCGCGTTCCACCACAGGGCGAGAAACGCCGCCAGCGCACCCGCTACCACCAGCGTGGTGGCGCTTACACCTGGAATTAACCAGCCGGGAATTAACGGCTGACGGCGCGTTGCCATTCGCTAATCCATGCCTGAc               >  1:898147/1‑251 (MQ=255)
                                                                                                                                                                                                                                      gAGGAATATCGCGGGTACGACAGAGAGCAGCGCCGAGAGAAACGCCTGCCAGAAGGAGAAGCGCACCACATGCCAC‑AGATAGCTGTCCTGCCAGACTGCCACCCCGTCATCCTGCGGCGAGTTCCACCACAGGGCGAGAAACGACGCCAGCTCAACCGCTACCACCAGCGTGGGGGCGCTTACAACGGGAATTACCCAGACGTGAATTAACGACTGACGGCGCGGTGCAATTCGCTAATGCATGCCTGAcc              >  2:746791/1‑250 (MQ=255)
                                                                                                                                                                                                                                          aaTATCGCGGGTACGACAGAGAGCAGCGCCGAGAGAAACGCCTGCCAGAAGGAGAAGCGCACCACATGCCAC‑AGATAGCTGTCCTGCCAGACTGCCACCCAGTCATCCTGCGGCGCGTTCCACCACAGGGCGAGACACGCCGCCAGCGCAACCGCTACCACCAGCGTGGTGGCGCTTACACCTGGAATTAACCAGCCGGGAATTAACGGCTGGCGGCGCGTTGCCATTTACTAATCCATGCCTGACGTtgt          >  2:490175/1‑251 (MQ=255)
                                                                                                                                                                                                                                           atatCGCGGGTACGACAGAGAGCAGCGCCGAGAGAAACGCCTGCCAGCAGGAGAAGCGCACCACATGCCAC‑AGATAGCTGTCCTGCCAGACTGCCACCCAGTCATCCTGCGGCGCGTTCCACCACAGGGCGAGAAACGCCGCCAGCGCAACCGCTACCACCAGCGTGGTGGCGCTTACACCTGGAATTAACCCGCCGGGAATTAACGGCTGGCGGCGCGTTGCCCTTCGCTCATCAAAGCCTGACGTtgtg         >  2:478753/1‑251 (MQ=255)
                                                                                                                                                                                                                                            tatCGCGGGTACGACAGAGAGCAGCGCCGAGAGAAACGCCTGCCAGAAGGAGAAGCGCACCACATGCCAC‑AGATAGCTGTCCTGCCAGACTGCCACCCAGTCATCCTGCGGCGCGTTCCACCACAGGGCGAGAAACGCCGCCAGCGCAACCGCTACCACCAGCGTGGTGGCGCTTACACCTGGAATTAACCAGCCGGGAATTAACGGCTGACGGCGCGTTGCCATTCGCTAATCCATGCCTGACGTTGTgc        >  1:191593/1‑251 (MQ=255)
                                                                                                                                                                                                                                                  gggTACGACAGAGAGCAGCTCCGAGAGAAACGCCTGCCAGAAGGAGAAGCGCACCACATGCCAC‑AGATAGCTGTCCTGCCAGACTGCCACCCAGTCATCCTGCGGCGCGTTCCACCACAGGGCGAGAAACGCCGCCAGCGCAACCGCTACCACCAGCGTGGTGGCGCTTACACCTGGAATTAACCAGCCGGGAATTAACGGCTGACGGCGCGTTGCCATTCGCTAATCCATGCCTGACGTTGTGCCGCCAc  >  1:29103/1‑251 (MQ=255)
                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                      
CATCGGCAGATTAAAAAACACATGGGCCAGCAAAATACCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGTGTTGCCAGCCAGCCC‑TGGCGACCATAGACGCTAAGA‑ATGCCGAAAACAGCGACCAACACCGGGAGGATCAAGGTCATTGCACACAGACGCAACAGCGCCAGCCGACCCGGAAAGCGCCTGCGATAGAGCGCGCGGGCGAGGAATATCGCGGGTATGACAGAGAGCAGTGCCGAGAGAAACGCCTGCCAGAAGGAGAAGCGCACCACATGCCAC‑AGATAGCTGTCCTGCCAGACTGCCACCCAGTCATCCTGCGGCGCGTTCCACCACAGGGCGAGAAACGCCGCCAGCGCAACCGCTACCACCAGCGTGGTGGCGCTTACACCTGGAATTAACCAGCCGGGAATTAACGGCTGACGGCGCGTTGCCATTCGCTAATCCATGCCTGACGTTGTGCCGCCAC  >  NC_000913/74063‑74553

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑