BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	74,081	C→T	V147V (GTG→GTA)	thiP ←	thiamine ABC transporter membrane subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	74,081	0	C	T	98.0%	306.6 / ‑2.0	103	V147V (GTG→GTA)	thiP	thiamine ABC transporter membrane subunit
Reads supporting (aligned to +/- strand): ref base C (1/1); new base T (45/54); total (47/56)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.94e-01

Only 100 of 105 total aligned reads displayed.

GGTCGCCTGCGGACCGCCCCCCAGCGATAGCACGGTGGCGAAGCTGGCGAAACAGAGCATAAAGATAAGCGCAGCAACCGGCGGGATTTGTCGCCGTAACCACGGCCATTCGACGAAGCGGAAAAAATGCCAGCTACGCATCCCAAGCTGGGCGGCAAGTTGACGCTGTTCGCCGGGGATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGCAGATTAAAAAACACATGGGCCAGCAAAATACCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGTGTTGCCAGCCAGCCCTGGCGAC‑CATAGACGCTAAGAATGCCGAAAACAGCGACCAACACCGGGAGGATCAAGGTCATTGCACACAGACGCAACAGCGCCAGCCGACCCGGAAAGCGCCTGCGATAGAGCGCGCGGGCGAGGAATATCGCGGGTATGACAGAGAGCAG  >  NC_000913/73850‑74320

ggttgccggCGGACCGCCGCCCAGCGATAGCACGGTGGCGAAGCTGGCGAAACAGAGCATAAAGATAAGTGCAGCAACCGGCGGGATTTGTCGCCGTAACCACGGCCATTCGACGAAGCGGAAAAAATGCCAGCCGCGCATCCCAAGCTGGGCGGCAAGCTGACGTTGTTCGCCGGGAATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGTAGATTAAAAAATACGTGCGCCAGCAAAATAc                                                                                                                                                                                                                               <  2:594362/243‑1 (MQ=255)

  gtgCCTGCGGACCCCCGCCCAGCGTTAGCACGGTGGCGAAGCTGGCGAAACAGAGCACAAAGATTAGTGACGCAACCTGCGGGAGTTGTCGCCGTACCCACGGCCATGCGACGAAGCGTACAAAATGCCAGCCGTGCATCCCAAGCTGGGCGGCAAGCTGACGTTGTTCGCCGGGAATGTTCTCCAGTGCCTGGAGTAATAAGCGGCTCGCCAGCGGTAGATTAAAAAATACGTGCGCCAGCAAAATACCt                                                                                                                                                                                                                             <  2:266175/249‑1 (MQ=255)

        gCGGACCGCCGCCCAGCGATAGCACGGTGGCGAAGCTGGCGAAACAGAGCATAAAGATAAGTGCAGCAACCGGCGGGATTTGTCGCCGTAACCACGGCCATTCGACGAAGCGGAAAAAATGCCAGCCGCGCATCCCGAGCTGGGCGGCAAGCTGACGTTGTTCGCCGGGAATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGTAGATTAAAAAATACGTGCGCCAGCAAAATACCTTGCa                                                                                                                                                                                                                         <  1:660318/249‑1 (MQ=255)

        gCGGACCGCCGCCCAGCGATAGCACGGTGGCGAAGCTGGCGAAACAGAGCATAAAGATAAGTGCAGCAACCGGCGGGATTTGTCGCCGTAACCACGGCCATTCGACGAAGCGGAAAAAATGCCAGCCGCGCATCCCAAGCTGGGCGGCAAGCTGACGTTGTTCGCCGGGAATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGTAGATTAAAAAATACGTGCGCCAGCAAAATACCTTGCAgg                                                                                                                                                                                                                       <  1:586278/251‑1 (MQ=255)

         cGGACCGCCGCCCAGCGATAGCACGGTGGCGAAGCTGGCGAAACAGAGCATAAAGATAAGTGCAGCAACCGGCGGGATTTGTCGCCGTAACCACGGCCATTCCACGAAACGGAAAAAATGCCAGCCGCCCATCCCAAGCTGGGCGGCAAGCTGACGTTGGTCGCCGGGAATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGTAGATTCAAAACTACGTGCGCCAGCAAAATACCTTGCAGGc                                                                                                                                                                                                                      >  1:631750/1‑251 (MQ=255)

            aCCGCCGCCCAGCGATAGCACGGTGGCGAAGCTGGCGAAACAGAGCATAAAGATAAGTGCAGCAACCGGCGGGATTTGTCGCCGTAACCACGGCCATTCGACGAAGCGGAAAAAATGCCAGCCGCGCATCCCAAGCTGGGCGGCAAGCTGACGTTGTTCGCCGGGAATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGTAGATTAAAAAATACGTGCGCCAGCAAAATACCTTGCAGGCCGt                                                                                                                                                                                                                   <  1:187495/251‑1 (MQ=255)

              cgccgcCCAGCGATAGCACGGTGGCGAAGCTGGCGAAACAGAGCATAAAGATAAGTGCAGCAACCGGCGGGATTTGTCGCCGGAACCACGGCCATTCGGCGAAGCGGAAAAAATGCCCGCCGCGCATCCCAAGCTGGGCGGCAAGCTGACGGTGTTCGCCGGGGATGTTTTCCAGTGCCCGGAGTAATAACCGGCTCGCCCTCGGTCGATTAAAAAATACGTGAGCCAGCAAAATACCTTGCAAGCCGt                                                                                                                                                                                                                   >  2:116783/1‑249 (MQ=255)

               gccgccaaGCGATAGCACGGGGGCGAAGCTGGCGAAACAGAGCTTAAAGATAAGTGCAGCAACCGGCGGGACTTGTCGCCGTAACCACGGCCATTCGACGAAGCGGAAAAAATGCCAGCCGCGCATCCCAAGCTGGGCGGCAAGCTGACGTTGTTCGCCGGGAAGGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGTAGATTAAAAAATACGTGCGCCAGCAAAATACCTTGCAGGCCGTAgg                                                                                                                                                                                                                <  2:108061/244‑1 (MQ=255)

                  gcccAGCGATAGCACGGTGGCGAAGCTGGCGAAACAGAGCATAAAGATAAGTGCAGCAACCGGCGGGATTTGTAGACGTAACCACGGCCATTCGGCGAAGCGGAAAAAATGCCAGCCGCGCATCCCAAGCTGGGCGGACAGCTGACGTTGTTCGCCGGGAATGTTTTCCGTTGCCTGGAGTAATAAACGGCTCGCCCTAGGGAGATTAAAAAATACGTGCGCCAGCAAAACACCTTTCAGGcaggaggggg                                                                                                                                                                                                             >  2:421600/2‑242 (MQ=255)

                    ccAGCGATAGCACGGTGGCGAAGCTGGCGAAACAGAGCATAAAGATAAGTGCAGCAACCGGCGGGATTTGTCGCCGTAACCACGGCCATTCGACGAAACGGAAAAAATGCCAGCCGCGCATCCCAAGCTGGGCGGCAAACCGACGTTGTACGCCGGGAATGTTTTCCAGTGCCGGGAGTAATAAGCGGCTCGCCCTCGGGAGAGTCAAAAATACGTTCCCCCGCCAAAAACCTTGCAGGCCGtgaggcga                                                                                                                                                                                                            >  2:302259/1‑243 (MQ=255)

                            aGCACGGTGGCGAAGCTGGCGAAACAGAGCATAAAGATAAGTGCAGCAACCGGAGGGATTTGTCGCCGTAACCACGGCCATTCGACGAAGCGGAAAAAATGACGGCCGCGCATCCCAAGCTGGGCGGCAAGCTGACGTAGTTCGCCGGGAAAGTTTTCCAGTGCATGGAGTAAAAAGAGGCTCGCCATCGGTAGATTAAAAAATACGTGAGCCAGCAAAATACCGTGCAGGCCGAAGGGCGAAAAGGTcc                                                                                                                                                                                                    >  1:550669/1‑250 (MQ=255)

                               ccGGTGGCGAAGCTGGCGAAACAGAGCATAAAGATAAGTGCAGCAACCGGCGGGATTTGTCGCCGTAACCACGGCCATTCGACGAAGCGGAAAAAATGCCAGCCGCGCATCCCAAGCTGGGCGGCAAGCTGACGTTGTTCGCCGGGAATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGTAGATTAAAAAATACGTGCGCCAGCAAAATACCTTGCAGGCCGTAGGGCGAAAAGGTCCACTc                                                                                                                                                                                                <  1:730117/250‑1 (MQ=255)

                                  gTGGCGAAGCTGGCGAAACAGAGCATAAAGATAAGTGCAGCAACCGGCGGGATTTGTCCCCGTAAACAAGGCCATTCGACGAAGCGGAAAAAATGCCAGCCGCGCATCCCAAGCTGGGCGGCAAGCTGACGGTGTTCGCCGGGAATGTTTTCCAGTGCCTGGAGTAATAAGAGGCTCGCCATCGGTAGATTAAAAAATACGTGCGCCAGCAAAATACCTTTCAAGCCGGAAGGCGAAAAGGGCCAATCCa                                                                                                                                                                                              >  1:601635/1‑250 (MQ=255)

                                     gCGAAGCTGGCGAAACAGAGCATAAAGATAAGTGCAGCAACCGGCGGGATTTGTCGCCGTAACCACGGCCATTCGACGAAGCGGAAAAAATGCCAGCCGCGCATCCCAAGCTGGGCGGCAAGCTGACGTTGTTCGCCGGGAATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGTAGATTAAAAAATACGTGCGCCAGCAAAATACCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGAcc                                                                                                                                                                                          <  2:811311/251‑1 (MQ=255)

                                       gAAGCTGGCGAAACAGAGCATAAAGATAAGTGCAGCAACCGGCGGAATTTGTCGCCGTAACCACGGCCATTTGACGAAGCGGCAAAAAAGCCAGCCGCGCCTCCCCAGCTGGGGGGCAAGCTGAAGGTGTTCGCCGGGGATGTTTTTCAGTGCCTGGAGTCATAAGCGGCCCGCCACCGGTAGCTTTAAAAATAAGTTCGCCTGTAAAATACCCTGCAGGCCGTAGGGGGAACAGGGCCACTCCAGACCgg                                                                                                                                                                                        >  2:283797/1‑250 (MQ=255)

                                                  aaCAGAGCATAAAGATAAGTGCAGCAACCGGCGGGATTTGTCGCCGTAACCACGGCAATTCGACGTAGCGGAAAAAATGCCAGGCGCGCCTCCCAAGCTGGGCGGCAAGCTGACGTTGTTCGCAGGGGATGGTTTCACGTGCCTGGAGTAATACGCCGCCCGCCATCGGAAGATTAAAAAATACGTGCGACAGCAAAATACCTTGAAGGACGTAGGGGGAAAAGGTCCACTCCAGACCACGCGATTGGc                                                                                                                                                                               >  1:67635/1‑249 (MQ=255)

                                                   aCAGAGCATAAAGAGTAGTGCAGCAACCGGCGGGATTTGTCGCCGTAACCACGGCCATTCGACGAAGCGGAAAAAATGCCAGCCGCGCATCCCAAGCTGGGGGGCAAGCTGACGTTGTTCGCCGGGAATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGTGGATTAAAAAATACGTGCGCCATCAAAATACCTTGCAGGCCGTAGGGCGAAAAGGGCCACTCCAGACCGAGCGATTGGCaga                                                                                                                                                                            <  1:440769/251‑1 (MQ=255)

                                                      gagCATAAAGATAAGTGCAGCAACCGGCGGGATTTGTCGCCGTAACCACGGCTATTCGACGAAGCGGAAAAAATGCCAGCCGCGCATCCCAAGCTGGGCGTCAAGCTGACGTTGTTCGCCGGGAATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGTAGATTAAAAAATACGTGCGCCAGCAAAATCCCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAgtg                                                                                                                                                                         <  1:172318/251‑1 (MQ=255)

                                                      gagCATAAAGATAAGTGCAGCAACCGGCGGGATTTGTCGCCGTAACCACGGCCATTCGACGAAGCGGAAAAAATGCCAGCCGCGCATCCCAAGCTGGGCGGCAAGCTGACGTTGTTCGCCGGGAATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGTAGATTAAAAAATACGTGCGCCAGCAAAATACCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCagaggg                                                                                                                                                                         >  1:19785/1‑249 (MQ=255)

                                                      gagCATAAAGATAAGTGCAGCAACCGGCGGGATTTGTCGCCGTAACCACGGCCATTCGACGAAGCGGAAAAAATGCCAGCCGCGCATCCCAAGCTGGGCGGCAAGCTGACGTTGTTCGCCGGGAATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGGAGATTAAAAAATACGTGCGCCAGCAAAATACCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCagaggg                                                                                                                                                                         >  1:455465/1‑249 (MQ=255)

                                                      gagCATAAAGATAAGTGCAGCAACCGGCGGGATTTGTCGCCGTAACCACGGCCATTCGACGAAGCGGAAAAAATGCCAGCCGCGCATCCCAAGCGGGGCGGCAAGCTGACGTTGTTCGCCGGGAATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGTAGATTAAAAAATACGTGCGCCAGCAAAATACCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAgtg                                                                                                                                                                         <  2:693665/251‑1 (MQ=255)

                                                        gCATAAAGATAAGTGCAGCAACCGGCGGGATTTGTCGCCGTAACCACGGCCATTCGACGAAGCGGAAAAAATGCCAGCCGCGCATCCCAAGCTGGGCGGCAAGCTGACGTTGTTCGCCGGGAATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGCAGATTAAAAAATACGTGCGCCAGCAAAATACCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGCCCGAGAGATTGGCAGAGTGtt                                                                                                                                                                       >  1:883426/1‑251 (MQ=255)

                                                         cATAAATATAAGTGCAACAACCGGCGGGATTTGTCGCCGGAACCACGGCCATCCGACGAAGCGGAAAAAATGCCAGCCGCGCATCCCAAGCTGGGCGGCAAGCTGACGTTGTTCGCCGGGAATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGTAGATTAAAAAATACGTGCGCCAGCAAAATACCGTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGTGtt                                                                                                                                                                       <  2:409018/250‑1 (MQ=255)

                                                              aGATAAGTGCAACAACCGGCGGGATTTGTCGCCGTAACCACGGCCATTCGACGAAGCGGGAAAAATGCCAGCCGCGCAGCCCAAGCTGGGCGGCAAGCTGACGTTGTTCGCCGGGAATGTTTGCCAGTGCCTGGAGTAATAAGCGGCTCGCCAGCGGTAGATTAAAAAATACGTGCGCCAGCAAAAGCCCGTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGTGTTgccagc                                                                                                                                                                 <  2:74493/251‑1 (MQ=255)

                                                                  aagtgcagcaACCGGCGGGATTTGTCGCCGTAACCACGGCCATTCGACGAAGCGGAAAAAATGCCAGCCGCGCATCCCAAGCTGGGCGGCAAGCTGACGTTGTTCGCCGGGAATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGTAGATTAAAAAATACGTGCGCCAGCAAAATACCTTGCAGGCCGTAGGGCGAAAAGGGCCACTCCAGACCGAGCGATTGGCAGAGTGTTGCcagccagc                                                                                                                                                             >  1:734036/5‑251 (MQ=255)

                                                                   agtgcagcaACCGGCGGGATTTGTCGCCGTAACCACGGCCATTCGACGAAGCGGAAAAAATGCCAGCCGCGCATCCCAAGCTGGGCGGCAAGCTGACGTTGTTCGCCGGGAATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGTAGATTAAAAAATACGTGCGCCAGCAAAATACCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGTGTTGCCagccagcc                                                                                                                                                            <  1:825788/248‑1 (MQ=255)

                                                                       cagcaACCGGCGGGATTTGTCGCCGTAACCACGGCCATTCGACGAAGCGGAAAAAATGCCAGCCGCGCATCCCAAGCTGGGCGGCAAGCTGACGTTGTTCGCCGGGAATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGTAGATTAAAAAATACGTGCGCCAGCAAAATACCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGTGTTGCCAGCCAGCCCTgg                                                                                                                                                        <  1:722793/251‑1 (MQ=255)

                                                                          caACCGGCGGGATTTGTCGCCGTAACCACGGCCATTCGACGAAGCGGAAAAAATGCCAGCCGCGCATCCCAAGCTGGGCGGCAAGCTGACGTTGTTCGCCGGGAATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGTAGATTAAAAAATACGTGCGCCAGCAAAATACCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGTGTTGCCAGCCAGCCCTGGCg                                                                                                                                                      >  1:841638/1‑250 (MQ=255)

                                                                            aCCGGCGGGATTTGTCGCCGTAACCACGGCCATTCGACGAAGCGGAAAAAATGCCATCCGCGCATCCCAAGCTGGTCGGCAAGCTGACGTTGTTCGCCGGGAATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGGAGATTAAAAAATACGTGCGCCAGCAAAATACCGTGCAGGCCGTAGGGCGAAAAGGGCCACTCCAGACCGAGCGATTGGCAGAGTGTTGCCAGCCAGCCCTGGCGAc‑c                                                                                                                                                  <  2:96920/251‑1 (MQ=255)

                                                                              cggcggGATTTGTCGCCGTAACCACGGCCATTCGACGAAGCGGGAAAAATGCCAGCCGCGCATCCCAAGCTGGGCGGCAAGCTGACGTTGTTCGCCGGGAATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGTAGATTAAAAAATACGTGCGCCAGCAAAATACCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGTGTTGCCAGCCAGCCCTGGCGAC‑Ca                                                                                                                                                 >  1:104803/1‑250 (MQ=255)

                                                                               ggcggTATTTGTCGCCGTAACCACGGCCATTCGACGAAGCGGAAAAAATGCCAGCCGCGCATCCCAAGCTGGGCGGCAAGCTGACGTTGTTCGCCGGGAATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGTAGATTAAAAAATACGTGCGCCAGCAAAATACCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGTGTTGCCAGCCAGCCCTGGCGAC‑CATa                                                                                                                                               <  1:941766/251‑1 (MQ=255)

                                                                               ggcggGATTTGTCGCCGTAACCACGGCCATTCGACGAAGCGGAAAAAATGCCAGCCGCGCATCCCAAGCTGGGCGGCAAGCTGACGTTGTTCGCCGGGAATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGTAGATTAAAAAATACGTGCGCCAGCAAAATACCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGTGTTGCCAGCCAGCCCTGGCGAC‑CAt                                                                                                                                                >  1:354854/1‑250 (MQ=255)

                                                                                  gggATTTGTCGCCGTAACCACGGCCATTCGACGAAGCGGAAAAAATGCCAGCCGCGCATCCCAAGCTGGGCGGCAAGCTGACGTTGTTCGCCGGGAATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGTAGATTAAAAAATACGTGCGCCAGCAAAATACCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGTGTTGCCAGCCAGCCCTGGCGAC‑CATaaac                                                                                                                                            >  1:442617/1‑248 (MQ=255)

                                                                                  gggATTTGTCGCCGTAACCACGGCCATTCGACGAAGCGGAAAAAATGCCAGCCGCGCATCCCAAGCTGGGCGGCAAGCTGACGTTGTTCGCCGGGAATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGTAGATTAAAAAATACGTGCGCCAGCAAAATACCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGGGTTGCCAGCCAGCCCTGGCGAC‑CATaaac                                                                                                                                            >  1:957837/1‑248 (MQ=255)

                                                                                   ggATTTGTCGCCGTAACCACGGCCATTCGACGAAGCGGAAAAAATGCCAGCCGCGCATCCCAAGCTGGGCGGCAAGCTGACGTTGTTCGCCGGGAATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGTAGATTAAAAAATACGTGCGCCAGCAAAATACCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGTGTTGCCAGCCAGCCCTGGCGAC‑CATAGACg                                                                                                                                           >  1:594716/1‑251 (MQ=255)

                                                                                    gATTTGTCGCCGTAACCACGGCCATTCGACGAAGCGGAAAAAATGCCAGCCGCGCATCCCAAGCTGGGCGGCAAGCTGACGTTGTTCGCCGGGAATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGTAGATTAAAAAATACGTGCGCCAGCAAAATACCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGTGTTGCCAGCCAGCCCTGGCGAC‑CATAGACGc                                                                                                                                          <  1:845810/251‑1 (MQ=255)

                                                                                      tttggtgcgttacccACGGCCTTGCGACGAAGCGGAAAACAGGCCAGCCGCGCAGCCCAAGCTGGGCGGCAAGATGACGTTGTTCGCCGGGAATGTTTTCCAGGGCCTGGAGTAATAAGCGGCTCGCCATCGGTAGATTAAAAAATACGTGCGCCAGCAAAATACCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGTGTTGCCATCCAGCCCTGGCGAC‑CATAGACGCt                                                                                                                                         <  2:835479/237‑1 (MQ=255)

                                                                                           cGCCGTAACCACGGCCATTCGACGAAGCGGAAAAAATGCCAGCTACGCATCCCAAGCTGGGCGGCAAGTTGACGCTGTTCGCCGGGGATGTTTTCCAGTGCCTGGAGTA‑TAAGCGGCTCGCCATCGGCAGATTAAAAAACACATGGGCCAGCAAAATACCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGTGTTGCCAGCCAGCCCTGGCGAC‑CATAGACGCTAAGAAt                                                                                                                                   <  1:278189/250‑1 (MQ=255)

                                                                                               gTAACCACGGCCATTCGACGAAGCGAAAAAAATGCCAGCCGCGCATCCCAAGCTGGGCGGCAAGCTGACGTTGTTCGCCGGGAATGTTTTCCAGTACCTGGAGTAATAAGCGGATCGCCATCGGTAGATTAAAAAAAACGTGCGCCAGCAAAATACCTTGCAGGCCGTAGGGCGAAAAGAGCCACTCCAGCCCGAGAGATTAGCAGCGTGGAGCCAGCCCGCCCAGGCGCC‑Cagagcagcgacgaatgccg                                                                                                                               >  2:827866/1‑233 (MQ=255)

                                                                                                 aacaaCGGCCATTCGACGAAGCGGAAAAAATGCCAGCCGCGCATCCCAAGACGGTCGGCAAGCTGACGTTGTTCGCCGGGAATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGGAGATTAAAAAATACGTGCGCCAGCAAAATACCTTGCAGGCCGTAGAGCGCAAAGGTCCACTCCAGCCCGAGCGATTGGACGAGTATTGCCAGCCGGCCCGGCCGAC‑CAACGACGCTACGaagaccga                                                                                                                              >  2:711373/5‑244 (MQ=255)

                                                                                                 aaCCACGGGCATTCGACGAAGCGGAAACAATGCCAGCCGCGCATCCCACGCTGGGCGGCAAGCTGACGTTGTTCGCCGGGAATGTTTTACAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGTAGATTAAGAAATAGGTGCGAAAGAAAAATTCCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCATACCGAGAGATTGGCAGTGTGATGGCAGCCAGCCCTGGCTCC‑CATATACGCTAAGAATACCGa                                                                                                                              >  2:596502/1‑250 (MQ=255)

                                                                                                 aaCCACGGCCATTCGACGAAGCGGAAAAAATGCCAGCCGCGCATCCCAAGCTGGGCGGCAAGCTGACGTTGTTCGCCGGGAATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGTAGATTAAAAAATACGTGCGCCAGCAAAATACCTTGCAGGCCGAAGGGCGAAAAGGTCCACTCCAGACCGCGCGATTGGCAGAGTGTTGCCAGCCAGCCCTGGCGAC‑CATAGACGCTAAGAATGCCGa                                                                                                                              >  1:617608/1‑250 (MQ=255)

                                                                                                 aaCCACGGCCATTCGACGAAGCGGAAAAAATGCCAGCCGCGCATCCCAAGCGGGGCGGCAAGCTGACGTTGTTCGCCGGGAATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGTAGATTAAAAAATACGGGCGCCAGCAAAATACCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGTGTTGCCAGCCAGCCCTGGCGAC‑CATAGACGCTAAGAATGCCGa                                                                                                                              <  2:764371/250‑1 (MQ=255)

                                                                                                          cATTCGATGAAGCGGAAAAAATGCCAGGCGCGCATCCCAAGCTGGGCGGCAAGCTGACGTTGTTCGCCGGGAATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCAGCGGTAGATTAAAAAATACGTGCGCCAGCAAAATACCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGTGTTGCCAGTCAGCCCTGGCGAC‑CATAGACGCTAAGAATGCCGAAAACAGcaac                                                                                                                    <  2:678659/251‑4 (MQ=255)

                                                                                                          cATTCGACGAAGCGGAAAAAATGCCAGCCGCGCATCCCAAGCTGGGCGGCAAGCTGACGTTGTTCGCCGGGAATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGTAGATTAAAAAATACGTGCGCCAGCAAAATACCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGTGTTGCCAGCCAGCCCTGGCGAC‑CATAGACGCTAAGAATGCCGAAAACAGcaac                                                                                                                    <  1:310878/251‑4 (MQ=255)

                                                                                                            ttCGACGAAGCGGAAAAAATGCCAGCCGCGCATCCCAAGCTGGGCGGCAAGCTGACGTTGTTCGCCGGGAATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGTAGATTAAAAAATACGTGCGCCAGCAAAATACCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCCGAGTGTTGCAAGCCAGCCCTGGCGAC‑CATAGACGCTAAGAATGCCGAAAACAGCAAcc                                                                                                                   >  1:44731/1‑250 (MQ=255)

                                                                                                             tCGACGAAGCGGAAAAAATGCCAGCCGCGCATCCCAAGCTGGGCGGCAAGCTGACGTTGTTCGCCGGGAATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGTAGATTAAAAAATACGTGCGCCAGCAAAATACCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGTGTTGCCAGCCAGCCCTGGCGAC‑CATAGACGCTAAGAATGCCGAAAACAGCAACCa                                                                                                                  <  2:767680/250‑1 (MQ=255)

                                                                                                             tCGACGAAGCGGAAAAAATGCCAGCCGCGCATCCCAAGCTGGGCGGCAAGCTGACGTTGTTCGCCGGGAATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGTAGATTAAAAAATACGTGCGCCAGCAAAATACCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGTGTTGCCAGCCAGCCCTGGCGAC‑CATAGACGCTAAGAATGCCGAAAACAGCAACCa                                                                                                                  <  2:354854/250‑1 (MQ=255)

                                                                                                                acgaAGCGGAAAAAATGCCAGCCGCGCATCCCAAGCTGGGCGGCAAGCTGACGTTGTTCGCCGGGAATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGTAGATTAAAAAATACGTGCGCCAGCAAAATACCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGTGTTGCCAGCCAGCCCTGGCGAC‑CATAGACGCTAAGAATGCCGAAACCAGCAACCAgcac                                                                                                              <  2:757967/251‑1 (MQ=255)

                                                                                                                 cgaAGCGGAAAAAAGGCCAGCCGCGCATCCCACGGGGGGCGGCAAGCTTCCGTGGGTCGTCGGGAATGTTTGCCAGTGCCTGGAGTAATAAGCGGGTCGCCATCGGTAGATTAAAAAATACGTGCGCCAGCAAAATACCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTCGCAGAGTGGTGCCAGCCAGCCCTCGTGAC‑CATAGAGGCTGAGAATGCAGGAATCAGCAACCAGCAcc                                                                                                             <  2:412251/251‑1 (MQ=255)

                                                                                                                     gCGGAAAAAATACCTGCCGCGCATCCCAATCTGGGCGGCAAGCTGACGTTGTTCGCCGGGAATGTTTTCCAGTGCCTGGCGTAATAAGAGGCTCGCCCTCGGTCGATTAAAAAATACGTGCGCCAGAAAAATACCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCAAGCGATAGGCAGAGTGTGAGCAGCCAGCCCCGGCGGCACA‑AGTCGCTGAGAATGCCGCAAACAGCAAcccgaaccgg                                                                                                           >  2:417515/1‑241 (MQ=255)

                                                                                                                      cGGAAAAAATGCCAGCCGCGCATCCCAAGCTGGGCGGCAAGCTGACGTTGTTCGCCGGGAATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGTAGATTAAAAAATACGTGCGCCAGCAAAATACCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGTGTTGCCAGCCAGCCCTGGCGAC‑CATAGACGCTAAGAATGCCGAACACAGCAACCAGCACCgggcg                                                                                                        >  1:172981/1‑249 (MQ=255)

                                                                                                                      cGGAAAAAATGCCAGCCGCGCATCCCAAGCTGGGCGGCAAGCTGACGTTGTTCGCCGGGAATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGTAGATTAAAAAATACGTGCGCCAGCAAAATACCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGTGTTGCCAGACAGCCCTGGCGAC‑CATAGACGCTAAGAATGCCGAAAACAGCAACCAGCACCggggg                                                                                                        >  2:416506/1‑249 (MQ=255)

                                                                                                                       ggAAAAAATGCCAGCCGCGCATCCCAAGCTGGGCGGCAAGCTGACGTTGGTCGCCGGGAATGTTTTCCAGGGCCTGGAGTAATAAGCGGCTCGCCATCGGTAGATTAAAAAATACGTGCGCCAGCAAAATACCGTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGTGTTGCCAGCCAGCCCTGGCGAC‑CATAGACGCTAAGAATGCCGAAAACAGCAACCAGCACCGggagg                                                                                                       <  2:569713/251‑1 (MQ=255)

                                                                                                                        gAAAAAATGCCAGCCGCGCATCCCAAGCTGGGCGGCAAGCTGACGTTGTTCGCCGGGAATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGTAGATTAAAAAATACGTGCGCCAGCAAAATACCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGTGTTGCCAGCCAGCCCTGGCGAC‑CATAGACGCTAAGAATGCCGAAAACAGCAACCAGCACCGggagg                                                                                                       >  1:832957/1‑250 (MQ=255)

                                                                                                                        gAAAAAATGCCAGCCGCGCATCCCAAGCTGGGCGGCAAGCTGACGTGGTTCGCCGGGAATGTTTTCCAGTGCCGGGAGTAATAAGCGGCTCGCCATCGGTAGATTAAAAAATACGTGCGCCAGCAAAATACCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGTGTTGCCAGCCAGCCCTGGCGAC‑CATAGACGCTAAGAATGCCGAAAACAGCAACCAGCACCGggagga                                                                                                      <  2:150563/251‑1 (MQ=255)

                                                                                                                          aaaaaGGCCAGCCGCGCATCCCAAGCTGGGCGGCAAGCTGACGTTGTTCGCCGGGAATGTTTTCCAGTGCCTGTAGTAATAAGCGGCTCGCCATCGGTAGATTAAAAAATACGTGCGCCAGCAAAATACCTGGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGTGTTGCCAGCCAGCCCTGGCGAC‑CATAGACGCTAAGAATGCCGAAAACAGCAACCAGCACCGGGAGGAtt                                                                                                    <  2:919819/251‑2 (MQ=255)

                                                                                                                              aTGCCAGCCGCGCCTCCCAAGCTGGGCGGCAAGCTGGCGTTGTTCGCCGGGAATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGTAGATTAAAAAATACGTGCGCCAGCAAAATACCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGTGTTGCCAGCCAGCCCTGGCGAC‑CATAGACGCTAAGAAAGCCGAAACCAGCAACCAGCACAgggcgggagaagg                                                                                                >  2:694755/1‑241 (MQ=255)

                                                                                                                                    gccgcacatctcaacatGGGCGGCAAGGTTACGTTGTTCGCCGGTAATGTTTGCCAGTGCCTGTAGTAATAAGCGGCTCTCCATCGGTAGATTAAAAAATACTTGCGCCAGCAAAATACCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGTGTTGCCAGCCAGCCCTGGCGAC‑CATAGACGCTAAGAATGCCGAAAACAGCAACCAGCACCGGGAGGATTAAGGTCATTg                                                                                          <  2:636149/235‑1 (MQ=255)

                                                                                                                                     ccgcGCATCCCAAGCTGGGCGGCAAGCTGACGTTGTTCGCCGGGAATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGTAGATTAAAAAATACGTGCGCCAGCAAAATACCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGTGTTGCCAGCCAGCCCTGGCGAC‑CATAGACGCTAAGAATGCCGAAAACAGCAACCAGCACCGGGAGGATTAAGGTCATTGc                                                                                         <  1:574470/248‑1 (MQ=255)

                                                                                                                                     ccgcGCATCCCAAGCTGGGCGGCAAGCTGACGTTGTTCGCCGGGAATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGTAGATTAAAAAATACGTGCGCCAGCAAAATACCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGTGTTGCCAGCCAGCCCTGGCGAC‑CATAGACGCTAAGAATGCCGAAAACAGCAACCAGCACCGGGAGGATTAAGGTCATTGc                                                                                         >  1:655436/4‑251 (MQ=255)

                                                                                                                                      cgcGCATCCCAAGGTGGGCGGAAAGCTGACGGTGTTCGCCGGGAATGTTGTCCAGTGCCTGGAGTAATAAGCGGCGCGCCAGCGGTAGATTAAAAAATACGTGCGCCAGCAAAATACCGTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGTGTTGCCAGCCAGCCCTGGCGAC‑CATAGACGCTAAGAATGCCGAAAACAGCAACCAGCACCGGGAGGATTAAGGTCATTGcg                                                                                        <  2:434128/249‑2 (MQ=255)

                                                                                                                                      cgcGCATCCCAAGCTGGGCGGCAAGCTGACGTTGTTCGCCGGGAATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGTAGATTAAAAAATACGTGCGCCAGCAAAATACCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGTGTTGCCAGCCAGCCCTGGCGAC‑CATAGACGCTAAGAATGCCGAAAACAGCAACCAGCACCGGGAGGATTAAGGTCCTTGcg                                                                                        >  2:163942/3‑250 (MQ=255)

                                                                                                                                        cGCATCCCAAGCGGGGCGGCAAGCTGACGTTGTTCGCCGGGAATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGTAGATTAAAAAATACGTGCGCCAGCAAAATACCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGTGTTGCCAGCCAGCCCTGGCGAC‑CATAGACGCTAAGAATGCCGAAAACAGCAACCAGCACCGGGAGGATTAAGGTCATTGcgca                                                                                      <  2:139514/251‑4 (MQ=255)

                                                                                                                                          cATCCCAAGCTTGGCGGCAAGCTGACGTTGTTCGCCGGGAATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGTAGATTAAAAAATACGTGCGCCAGCAAAATACCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGTGTTGCCAGCCAGCCCTGGCGAC‑CATAGACGCTAAGAATGCCGAAAACAGCAACCAGCACCGGGAGGATTAAGGTCATTGcgcac                                                                                     >  1:707564/1‑250 (MQ=255)

                                                                                                                                           aTCCCAAGCTGGGCGGCAAGCTGACGTTGTTCGCCGGGAATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGTAGATTAAAAAATACGTGCGCCAGCAAAATACCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGTGTTGCCAGCCAGCCCTGGCGAC‑AATAGACGCTAAGAATGCCGAAAACAGCAACCAGCACCGGGAGGATTAAGGTCATTGcgcaccg                                                                                   >  2:778581/1‑249 (MQ=255)

                                                                                                                                            ccccaatcggggCGGCAAGCGGACGTTGTTCGCCGGGAAGGGTGTCCAGTGCCTGGAGTAATAAGCGGCTCTCCATCGGTAGATTAAAAAATACGTGCGCCAGCAAAATACCTGGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGTAGAGTGTTGCCAGCCAGCCCTGGCGAC‑CATAGACGCTAAGAATGCCGAAAACAGCAACCAGCACCGGGAGGATTAAGGTCATTGCGCACAGa                                                                                  <  2:205853/242‑1 (MQ=255)

                                                                                                                                             cccAAGCTGGGCGGCAAGCCGACGTTGTTCGCCGGGAATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGTAGATTAAAAAATACGTGCGCCAGCAAAATACCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGTGTTGCCAGCCAGCCCTGGCGAC‑CATAGACGCTAAGAATGCCGAAAACAGCAACCAGCACCGGGAGGATTAAGGTCATTGCGCACAGAc                                                                                 <  2:67083/251‑1 (MQ=255)

                                                                                                                                                aaGCTGGGCGGCAAGCTGACGTTGTTCGCCGGGAATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGTAGATTAAAAAATACGTGCGCCAGCAAAATACCTTGCAGGCCGTAGGGCGAAAAGGTCCACGCCAGACCGAGCGAGTGGCAGAGTGTTGCCAGCCAGCCCTGGCGAC‑CATAGACGCTAAGAATGCCGAAAACAGCAACCAGCACCGGGAGGATTAAGGTCATTGCGCACAGACGCa                                                                              <  1:156958/251‑1 (MQ=255)

                                                                                                                                                    ggggCGGCAAGCTGACGTTGTTCGCCGGGAATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGTAGATTAAAAAATACGTGCGCCAGCAAAATACCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGTGTTGCCAGCCAGCCCTGGCGAC‑CATAGACGCTAAGAATGCCGAAAACAGCAACCAGCACCGGGAGGATTAAGGTCATTGCGCACAGACGCAACAg                                                                          <  1:184351/250‑1 (MQ=255)

                                                                                                                                                    ggggCGGCAAGCGGACGTTGTTCGCCGGGAATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGTAGATTAAAAAATACGTGCGCCAGCAAAATACCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGTGTTGCCAGCCAGCCCTGGCGAC‑CATAGACGCTAAGAATGCCGAAAACAGCAACCAGCACCGGGAGGATTAAGGTCATTGCGCACAGACGCAACAg                                                                          <  2:196406/250‑1 (MQ=255)

                                                                                                                                                     gggCGGCAAGCTGACGTTTGTCGCCGGGAATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGTAGATTAAAAAATACGTGCGCCAGCAAAATACCTTGCATGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGTGTTGCCAGCCAGCCCTGGCGAC‑CATAGACGCTAAGAATGCCGAAAACAGCAACCAGCACCGGGAGGATTAAGGTCATTGCGCACAGACGCAACAgc                                                                         <  1:883518/251‑1 (MQ=255)

                                                                                                                                                     gggCGGCAAGCTGACGTTGTTCGCCGGGAATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCAACGGTAGATTAAAAAATACGTGCGCCGGCAAAATACCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGTGTTGCCAGCCAGCCCTGGCGAC‑CATAGACGCTAAGAATGCCGAAAACAGCAACCAGCACCGGGAGGATTAAGGTCATTGCGCACAGACGCAACa                                                                           <  2:19785/249‑1 (MQ=255)

                                                                                                                                                        gggcAAGCTGACGTTGTTCGCCGGGAATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGTAGATTAAAAAATACGTGCGCCAGCAAAATACCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGTGTTGCCAGCCAGCCCTGGCGAC‑CATAGACGCTAAGAATGCCGAAAACAGCAACCAGCACCGGGAGGATTAAGGTCATTGCGCACAGACGCAACAGCGcc                                                                      <  1:946775/250‑1 (MQ=255)

                                                                                                                                                           cAAGCTGACGTTGTTCGCCGGGAATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGTAGATTAAAAAATACGTGCGCCAGCAAAATACCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGTGTTGCCAGCCAGCCCTGGCGAC‑CATAGACGCTAAGAATGCCGAAAACAGCAACCAGCACCGGGAGGATTAAGGTCATTGCGCACAGACGCAACAGCGCCAGc                                                                   <  2:119386/251‑1 (MQ=255)

                                                                                                                                                            aagctGACGTTGTTCGCCGGGAATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGTAGATTAAAAAATACGTGCGCCAGCAAAATACCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGTGTTGCCAGCCAGCCCTGGCGAC‑CATAGACGCTAAGAATGCCGAAAACAGCAACCAGCACCGGGAGGATTAAGGTCATTGCGCACAGACGCAACAGCGCCAGcc                                                                  >  1:899348/5‑251 (MQ=255)

                                                                                                                                                             agctGACGTTGTTCGCCGGGAATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGTAGATTAAAAAATACGTGCGCCAGCAAAATACCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGTGTTGCCAGCCAGCCCTGGCGAC‑CGTAGACGCTAAGAATGCCGAAAACAGCAACCAGCACCGGGAGGATTAAGGTCATTGCGCACAGACGCAACAGCGCCAGCCg                                                                 >  1:878430/4‑251 (MQ=255)

                                                                                                                                                             agctGACGTTGTTCGCCGGGAATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGTAGATTAAAAAATACGTGCGCCAGCAAAATACCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGTGTTGCCAGCCAGCCCTGGCGAC‑CATAGACGCTAAGAATGCCGAAAACAGCAACCAGCACCGGGAGGATTAAGGTCATTGCGCACAGACGCAACAGCGCCAGCCg                                                                 <  1:351334/248‑1 (MQ=255)

                                                                                                                                                                    gttGTTCGCCGGGAATGTTTTCCAGAGCCTGGAGTAATAAGCGGCTCGCCATCGGTAGCTTAAAAAATACGTGCGCCAGCAAAATACCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGTGTTGCCAGCCAGCCCTGGCGAC‑CATAGACGCTAAGAATGCCGAAAACAGCAACCAGCACCGGGAGGATTAAGGTCATTGCGCACAGACGCAACAGCGCCAGCCGACCCgg                                                           >  1:619812/3‑250 (MQ=255)

                                                                                                                                                                         gctccgtgaaTGTTTTCCAGGTCCTGGAGTAATAAGCGGTTCGCAATAGGTAGATTAAAAAATCCGTGCCACAACAAAATACCTGGCAGTCCGTAGGGCGAAAAGGCCCACCCCAGACCGAGCGATTGGCAGAGTGTGGCCAGCCAGCCCTGGCGAC‑CATAGACGCTAAGAATGCCGAAAACAGCAACCAGCAGCGGGAGGATTAATGTCATTGCGCACAGACGCAACAGCGCCAGCCGACCCGGAAAgcg                                                     <  2:969812/242‑1 (MQ=255)

                                                                                                                                                                          cGCCGGGAATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGTAGATTAAAAAATACGTGAGCCAGCAAAATACCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGTGTTGCAAGCCAGCCCTGGCGAC‑AATAGACGCTAAGAATGCCGAAACCAGCAACCAGCACCGGGAGGATTAAGGTCATTGCGCACAGACGCACCAGCGCCAGCCGACCCGGAAAgcgc                                                    >  1:355785/1‑251 (MQ=255)

                                                                                                                                                                           gccggtaaTGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGTAGATTAAAAAATACGTGCGCCAGCAAAATACCTTGCAGGCCGTAGGGCGAACAGGTCCACTCCAGACCGCGCGATTAGCTGAGTGTTGCCAGCCAGCCCTGGCGAC‑CATAAACGCTAAGAATGCCGAAACCAGCCACCAGCCCCGGGAGGATTAAAGTCATTGCGCACAGCCACAACAGCACCAGCCGACCCGGAGAGCGcc                                                   >  2:433241/8‑251 (MQ=255)

                                                                                                                                                                              gggaaTGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGTAGATTAAAAAATACGTGCGCCAGCAAAATACCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGTGTTGCCAGCCAGCCCTGGCGAC‑CATAGACGCTAAGAATGCCGAAAACAGCAACCAGCACCGGGAGGATTAAGGTCATTGCGCACAGACGCAACAGCGCCAGCCGACCCGGAAAGCGCCTGc                                                >  1:685257/5‑251 (MQ=255)

                                                                                                                                                                               ggaaTGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGTAGATTAAAAAATACGTGCGCCAGCAAAATACCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGTGTTGCCAGCCAGCCCTGGCGAC‑CATAGACGCTAAGAATGCCGAAAACAGCAACCAGCACCGGGAGGATTAAGGTCATTGCGCACAGACGCAACAGCGCCAGCCGACCCGGAAAGCGCCTGCg                                               <  1:553505/248‑1 (MQ=255)

                                                                                                                                                                                       ttCCAGGGCCTGGAGTAATAAGCGGCTCGCCATCGGTAGATTAAAAAATACGTGCGCCAGCAAAATCCCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGTGTTGCCAGCCAGCCCTGGCGAC‑CATAGACGCTAAGAATGCCGAAAACAGCAACCAGCACCGGGAGGATTAAGGTCATTGCGCACAGACGCAACAGCGCCAGCCGACCCGGAAAGCGCCTGCGATAGAgc                                        <  2:923249/250‑1 (MQ=255)

                                                                                                                                                                                        cccAGTGCCTGGAGTAATAAGCGGCGCGCCATCGGTAGATGAAAAAATACGTGCGCCAGCAAAATACCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGTGTTGCCAGCCAGCCCTGGCGAC‑CATAGACGCTAAGAATGCCGAAAACAGCAACCAGCACCTGGAGGATTAAGGTCAGTGCGCACAGACGCAACAGCGCCAGCCGACCCGGAAAGCGCCTGCGATAGAgcgc                                      <  1:925162/250‑1 (MQ=255)

                                                                                                                                                                                           aGTGCCTGGAGTAATAAGCGGCTCGCCATCGGTAGATTAAAAAATACGTGCGCCAGCAAAATACCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGTGTTGCCAGCCAGCCCTGGCGAC‑CATAGACGCTAAGAATGCCGAAAACAGCAACCAGCACCGGGAGGATTAAGGTCATTGCGCACAGACGCAACAGCGCCAGCCGACCCGGAAAGCGCCTGCGATAGAGcgcgcg                                   >  1:184183/1‑251 (MQ=255)

                                                                                                                                                                                           aGTGCCTGGAGGAATAAGCGGCTCGCCATCGGTAGATTAAAAAATACGTGCGCCAGCAAAATACCTTGCAGGCCGTAGGGCGAAACGGTCCACTCCAGACCGAGCGATTGGCAGAGAGTTGCCAGCCAGCCCTGGCGAC‑CATAGAAGCTAAGAATGCCGAAAACAGCAACCAGCACCGGGAGGATTAAGGTCATTGCGCACAGACGCAACAGCGCCAGCCGAACCGGAAAGAGCCTGCGAGAGAgcgcacg                                   >  2:40951/1‑248 (MQ=255)

                                                                                                                                                                                            gTGCCGGGAGTAAGAAGCTGCTCTCCATCGTTAGATTAAAAAATACGTGCGCCAGCACAATCCCTTGCATGCCGTAGGGCGAGAAGGTCCACTCCCGACCGGGCTATTGTCAGAGTGTTGCCAGCCAGCCCTGGCGAC‑CATAGACGCTAAGAATGCCGAAAACAGCAACCAGCACCGGGAGGATTAAGGTCATTGCGCACAGAAGCAACAGCGCCAGCCGACCCGGAAAGCGCCTGCGATAGATCGCGCgg                                  <  1:966265/251‑1 (MQ=255)

                                                                                                                                                                                             tGCCTGGAGTAATAAGCGGCTCGCCATCGGTAGATTAAAAAATACGTGCGCCAGCAAAATACCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGTGTTGCCAGCCAGCCCTGGCGAC‑CATAGACGCTAAGAATGCCGAAAACAGCAACCAGCACCGGGAGGATTAAGGTCATTGCGCACAGACGAAACAGCGCCAGCCGACCCGGAAAGCGCCTGCGATAGCGCGCGCggg                                 >  1:598448/1‑251 (MQ=255)

                                                                                                                                                                                                       aataaGCGGCTCGCCATCGGTAGATTAAAAAATACGTACGCCAGCAAAATACCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGTGTTGCCAGCCAGCCCTGGCGAC‑CATAGACGCTAAGAATGCCGAAAACAGCAACCAGCACCGGGAGGATTAAGGTCATTGCGCACAGACGCAACAGCGCCAGCCGACCCGGAAAGCGCCTGAGAAAGAGCGCGCGGGCGAGGAatat                       >  1:319683/1‑251 (MQ=255)

                                                                                                                                                                                                        ataaGCGGCTCGCCATCGGTAGATTAAAAAATACGTGCGCCAGCAAAATACCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGTGTTGCCAGCCAGCCCTGGCGAC‑CATAGACGCTAAGAATGCCGAAAACAGCAACCAGCACCGGGAGGATTAAGGTCATTGCGCACAGACGCAACAGCGCCAGCCGACCCGGAAAGCGCCTGCGATAGAGCGCGCGGGCGAGGAATATc                      <  1:538834/251‑1 (MQ=255)

                                                                                                                                                                                                         taaGCGGCTCGCCATCGGTAGATTAAAAAATACGTGCGCCAGCAAAATACCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGTGTTGCCAGCCAGCCCTGGCGAC‑CATAGACGCTAAGAATGCCGAAAACAGCAACCAGCACCGGGAGGATTAAGGTCATTGCGCACAGACGCAACAGCGCCAGCCGACCCGGAAAGCGCCTGCGATAGAGCGCGCGGGCGAGGAATATcg                     >  1:347173/1‑251 (MQ=255)

                                                                                                                                                                                                              ggCTCGCCATCGGTAGATTAAAAAATACGTGCGCCAGCAAAATACCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGTGTTGCCAGCCAGCCCTGGCGAC‑CATAGACGCTAAGAATGCCGAAAACAGCAACCAGCACCGGGAGGATTAAGGTCATTGCGCACAGACGCAACAGCGCCAGCCGACCCGGAAAGCGCCTGCGATAGAGCGCGCGGGCGAGGAATATCGCgggg                >  1:32092/1‑250 (MQ=255)

                                                                                                                                                                                                               gCTCGCCAACGGTAGATTAAAAAAGACGGGCGCCAGCAAAATACCTTGCAGGCCGTAGGGCGAAAAGGTCCACGCCAGACCGAGCGATTGGCAGTGTGTTGCCAGCCATCCCTGGCGAC‑CATAGACGCTAAGAATGCCGAAAACAGCAACCAGCACCGGGAGGATTAAGGTCATTGCGCACAGACGCAACAGCGCCAGCCGACCCGGAAAGCGCCTGCGATAGAGCGCGCGGGCGAGGAATATCGCGGGTa               <  2:875593/251‑1 (MQ=255)

                                                                                                                                                                                                                cTCGCCATCGGCAGATTAAAAAACACATGGGCCAGCAAAATACCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGTGTTGCCAGCCAGCCCTGGCGAC‑CATCGACGCTAAGAATGCCGAAAACAGCGACCACCACCGGGAGGATAAAGGTCATTGCACACAGACGCAACAGCGCCAGCCGACCCGGACAGCGCCTGCGATAGAGCGCGCGGGCGAGGAATATCGCgggga               >  2:42384/1‑248 (MQ=255)

                                                                                                                                                                                                                     cATCGGTAGATTAAAAAATAAGTGCGCCAGCAAAATACCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGCGTGTTGCCAGCCAGCCCTGGCGAC‑CATAGACGCTAAGAATGCCGAAAACAGCAACCAGCACCGGGAGGATTAAGGGAATTGCGCACAGACGCAACAGCGCCAGCCGACCCGGAAAGCGCCTGCGATAGAGCGCGCGGGCGCGGAATGTCGCGGGTacggc           >  2:284868/1‑245 (MQ=255)

                                                                                                                                                                                                                      aTCGGTAGATTAAAAAATACGTGCGCCAGCAAAATACCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGTGTTGCCAGCCAGCCCTGGCGAC‑CATAGACGCTAAGAATGCCGAAAACAGCAACCAGCACCGGGAGGATTAAGGTCATTGCGCACAGACGCAACAGCGCCAGCCGACCCGGAAAGCGCCTGCGCTAGAGCGCGCGGGCGAGGCATATCGCGGGTACGAc           >  1:874596/1‑248 (MQ=255)

                                                                                                                                                                                                                        cggtaGATTAAAAAATACGTGCGCCAGCAAAATACCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGTGTGGCCAGCCAGCCCTGGCGAC‑CATAGACGCTAAGAATGCCGAAAACAGCAACCAGCACCGGGAGGATTAAGGTCATTGCGCACAGACGCAACAGCGCCAGCCGACCCGGAAAGCGCCTGCGATAGAGCGCGCGGGCGAGGAATATCGCGGGTACGACagaga      <  1:544587/247‑1 (MQ=255)

                                                                                                                                                                                                                                       cacaTGGGCCAGCAAAATCCCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGTGTTGCCAGCCAGCCCTGGCGAC‑CATAGACGCTAAGAATGCCGAAAACAGCGACCAACACCGGGAGGATCAAGGTCATTGCACACAGACGCAACAGCGCCAGCCGACCCGGAAAGCGCCTGCGATAGAGCGCGCgg                                  <  2:494190/208‑1 (MQ=255)

GGTCGCCTGCGGACCGCCCCCCAGCGATAGCACGGTGGCGAAGCTGGCGAAACAGAGCATAAAGATAAGCGCAGCAACCGGCGGGATTTGTCGCCGTAACCACGGCCATTCGACGAAGCGGAAAAAATGCCAGCTACGCATCCCAAGCTGGGCGGCAAGTTGACGCTGTTCGCCGGGGATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGCAGATTAAAAAACACATGGGCCAGCAAAATACCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGTGTTGCCAGCCAGCCCTGGCGAC‑CATAGACGCTAAGAATGCCGAAAACAGCGACCAACACCGGGAGGATCAAGGTCATTGCACACAGACGCAACAGCGCCAGCCGACCCGGAAAGCGCCTGCGATAGAGCGCGCGGGCGAGGAATATCGCGGGTATGACAGAGAGCAG  >  NC_000913/73850‑74320

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`