BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	73,744	A→G	L260L (TTG→CTG)	thiP ←	thiamine ABC transporter membrane subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	73,744	0	A	G	98.0%	520.9 / ‑1.9	156	L260L (TTG→CTG)	thiP	thiamine ABC transporter membrane subunit
Reads supporting (aligned to +/- strand): ref base A (2/1); new base G (71/80); total (74/82)
Fisher's exact test for biased strand distribution p-value = 6.04e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.58e-01

Only 100 of 158 total aligned reads displayed.

CCTGCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTACCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCGGGCGCAATGGCCTTACTCAATCGCTGACTCAACAGCACCAGCCCGAGGCAGCACACCATCTGGAGCA‑GCGCCAGCATTGCCGCGCGGGCAGGATCGTAGTCGTAACTCAGCGCCTGATAGATTGCCAGCTCGATAGTGGTCGCCTGCGGACCGCCCCCCAGCGATAGCACGGTGGCGAAGCTGGCGAAACAGAGCATAAAGATA‑AGCGCAGCAACCGGCGGGATTTGTCGCCGTAACCACGGCCATTCGACGAAGCGGAAAAAATGCCAGC  >  NC_000913/73497‑73983

ccTGCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCACCTGGCGATTTAGCCCATCGACGATCACCGCCAGCAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTTGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCTGGCGCAATGGCCTTACTCAATCGCTGACTcagcag                                                                                                                                                                                                                                                <  1:597229/251‑1 (MQ=255)

 cTGCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTAGCCCATCGACGATCACCGCCAGCAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCTGGCGCAATGGCCTTACTCAATCGCTGACTCagcagc                                                                                                                                                                                                                                               >  1:577473/1‑251 (MQ=255)

     cgccAGCGCAATACGCAACGGGGGCCCCAGCGCCTGCCACAGCCCCGGTTGGGCCAGCACTTCCGGCAACTGGCGTTTTAGCCCATCGACGATCACCGCCAGCAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTTGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCTGGCGCAATGGCCTTACTCAATCGCTGACTCAGCAGCACCa                                                                                                                                                                                                                                           <  1:628941/251‑1 (MQ=255)

     cgccAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTAGCCCATCGACGATCACCGCCAGCAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCAGGCGCAATGGCCTTAATCAATCGCTGACTCAGCAGCAcc                                                                                                                                                                                                                                            >  1:678659/1‑250 (MQ=255)

       cccgcgcAATACGCAACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTAGCCCATCGACGATCACCGCCAGCCACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTCACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAACCTTGCAGCAGCGTGGTGCCTGGCGCCATGGCATTACTCAATCGCTGAATCACCAGCACCaac                                                                                                                                                                                                                                         >  2:553505/4‑249 (MQ=255)

        cAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTAGCCCATCGACGATCACCGCCAGCAACGGGGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCTGGCGCAATGGCCTTACTCAATCGCTGACTCCGCAGCAcccacc                                                                                                                                                                                                                                        >  2:187495/1‑247 (MQ=255)

                   gCAACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTAGCCCATCGACGATCACCGCCAGCAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCTGGCGCAATGGCCTTACTCAATCGCTGACTCAGCAGCACCAACCCGAGGCAacac                                                                                                                                                                                                                             >  1:945537/1‑251 (MQ=255)

                   ccacggggtcccacggccccTGCCCCAGCACGGTTTGGGCCAGCACTTCCGGCACCGGGCGATTAAGCCCATCGACGATCACCGCCAGCAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCTGGCGCAATGGCCTTACTCAATCGCTGACTCAGCAGCACCAACCCGAGGCAacac                                                                                                                                                                                                                             <  1:87812/233‑1 (MQ=255)

                        gAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTAGCCCATCGACGATCACCGCCAGCAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCTGGCGCAATGGCCTTACTCAATCGCTGACTCAGCAGCACCAACCCGAGGCAACACACCAt                                                                                                                                                                                                                        >  1:893760/1‑251 (MQ=255)

                         gggcccccaGCGCCTGCCACAGCACCGGTTGGCCCAGCACTTCCGGCAACTGGCGATTTAGCCCATCGACGATCACCGCCAGCAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCTGGCGCAATGGCCTTACTCAATCGCTGACTCAGCAGCACCAACCCGAGGCAACACACCATc                                                                                                                                                                                                                       <  1:525558/244‑1 (MQ=255)

                          ggTCCACAGCGCCTGCCACAGCCCCGTTTGTGCCAGCCCTTCCGGCAACTGGCGATTTAGCCCACCGACGATCACCGCCAGCAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCTGGCGCAATGGCCTTACTCAATCGCTGACTCAGCAGCACCAACCCGAGGCAACACACCATCt                                                                                                                                                                                                                      <  1:227205/251‑1 (MQ=255)

                                    gcCTGCCAAAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTAGACCATCGACGATCACCGCCAGCAACGGTGGCAGCCACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGAGCCTGGCGCAATGGCCTTACTCAATCGCTGACTCAGCAGAACCAACCCGCGGCACCACACCACCTGGATCA‑gcgc                                                                                                                                                                                                           >  2:77157/1‑251 (MQ=255)

                                        cccACAGCACCGGTTGTGCCAGCACTTCCGGCAACGGGCGATTTAGCCCACCGACGATCACCGCCAGCAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCTGGCGCAATGGCCTTACTCAATCGCTGACTCAGCAGCACCAACCCGAGGCAACACACCATCTGGATCA‑GCGCCAGc                                                                                                                                                                                                       <  1:961730/250‑1 (MQ=255)

                                          cacaGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTAGCCCATCGACGATCACCGCCAGCAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCTGGCGCAATGGCCTTACTCAATCGCTGACTCAGCAGCACCAACCCGAGGCAACACACCATCTGGATCA‑GCGCCAGCAt                                                                                                                                                                                                     >  1:757967/1‑251 (MQ=255)

                                            caGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTAGCCCATCGACGATCACCGCCAGCAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCTGGCGCAATGGCCTTACTCAATCGCTGACTCAGCAGCACCAACCCGAGGCAACACACCATCTGGATCA‑GCGCCAGCAtcg                                                                                                                                                                                                   >  1:693665/1‑249 (MQ=255)

                                            caGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTAGCCCATCGACGATCACCGCCAGCAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCTGGCGCAATGGCCTTACTCAATCGCTGACTCAGCAGCACCAACCCGAGGCAACACACCATCTGGATCA‑GCGCCAGCAtcg                                                                                                                                                                                                   >  1:969812/1‑249 (MQ=255)

                                               cACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTAGCCCATCGACGATCACCGCCAGCAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCTGGCGCAATGGCCTTACTCAATCGCTGACTCAGCAGCACCAACCCGAGGCAACACACCATCTGGATCA‑GCGCCAGCATCGCcg                                                                                                                                                                                                >  1:731936/1‑251 (MQ=255)

                                                aCCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTAGCCCATCGACGATCACCGCCAGCAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCTGGCGCAATGGCCTTACTCAATCGCTGACTCAGCAGCACCAACCCGAGGCCACACACCATCTGGATCA‑GCGCCAGCATCGCcgg                                                                                                                                                                                               >  2:238870/1‑250 (MQ=255)

                                                   gtttGTGCCAGCCCTTCCGGCAACTGGCGTTTTAGCCCATCGACGATCACCGCCAGCAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCTGGCGCAATGGCCTTACTCAATCGCTGACTCAGCAGCACCAACCCGAGGCAACACACCATCTGGATCA‑GCGCCAGCATCGCcgcgcg                                                                                                                                                                                            <  1:909386/249‑1 (MQ=255)

                                                       gtgCCAGCACTTCCGGCAACTGGCGATTTAGCCCATCGACGATCACCGCCAGCAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCTGGCGCAATGGCCTTACTCAATCGCTGACTCAGCAGCACCAACCCGAGGCAACACACCATCTGGATCA‑GCGCCAGCATCGCCGCGCGGGc                                                                                                                                                                                         >  1:108061/1‑250 (MQ=255)

                                                         gCCAGCACTTCCGGCAACTGGCGATTTAGCCCATCGACGATCACCGCCAGCAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCTGGCGCAATGGCCTTACTCAATCGCTGACTCAGCAGCACCAACCCGAGGCAACACACCATCTGGATCA‑GCGCCAGCATCGCCGCGCGGGCAgg                                                                                                                                                                                      >  1:509575/1‑251 (MQ=255)

                                                          ccAGCACTTCCGGCAACTGGCGATTTAGCCCATCGACGATCACCGCCAGCAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCTGGCGCAATGGCCTTACTCAATCGCTGACTCAGCAGCACCAACCCGAGGCAACACACCATCTGGATCA‑GCGCCAGCATCGCCGCGCGGGCAgg                                                                                                                                                                                      >  1:105678/1‑250 (MQ=255)

                                                           cAGCACTTCCGGCAACTGGCGATTTAGCCCATCGACGATCACCGCCAGCAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCTGGCGCAATGGCCTTACTCAATCGCTGACTCAGCAGCACCAACCCGAGGCAACACACCATCTGGATCA‑GCGCCAGCATCGCCGCGCGGGCAGGAt                                                                                                                                                                                    >  1:247155/1‑251 (MQ=255)

                                                           aaGCACTTCCGGCAACTGGCGATTTAGCCCATCGACGATCACCGCCAGCAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCACATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCTGGCGCAATGGCCTTACTCAATCGCTGACTCAGCAGCACCAACCCGAAGCACCACACCATCTGGATCA‑GCGCCCGCATCGCCGCGCGGGCAGGAt                                                                                                                                                                                    >  2:586278/2‑251 (MQ=255)

                                                            aGCACTTACGGCAACTGGCGATTTAGCCCATCGACGATCACCGCCAGCAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTCACACCGTGTCGCAAGTGAGGAAATGCAGACGATCGTCCGGGGCGCGCCAGCCTTGCAGCAGCGGGGAGCCTGGCGCACTGGCCTTCCGCAATCGCAGAGTCAGCAGCACCACCCCGAGGAACAACACCATC‑GGGGCAAGCGCCAGCATCGCCGCGCGGGCAGGagc                                                                                                                                                                                   >  2:722793/1‑249 (MQ=255)

                                                               aCTTCCGGCAACTGGCGATTTAGCCCATCGACGATCACCGCCAGCAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCTGGCGCAATGGCCTTACTCAATCGCTGACTCAGCAGCACCAACCCGAGGCAACACACCATCTGGATCA‑GCGCCAGCATCGCCGCGCGGGCAGGATCGt                                                                                                                                                                                 >  1:983258/1‑250 (MQ=255)

                                                                  cccGGCAACGGGCGTTTTAGCCCATCGACGATCACCGCCAGCAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGACGCGCCAGCCTTGCAGCAGCGTGGTGCCTGGCGCAATGGCCTTACTCAATCGCTGACTCAGCAGCACCAACCCGAGGCAACACACCATCTGGATCA‑GCGCCAGCATCGCCGCGCGGGCAGGATCGTAGt                                                                                                                                                                              <  1:906293/249‑1 (MQ=255)

                                                                       cAACGGGCGATTTAGCCCATCGACGATCACCGCCAGCAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCTGGCGCAATGGCCTTACTCAATCGCTGACTCAGCAGCACCAACCCGAGGCAACACACCATCTGGATCA‑GCGCCAGCATCGCCGCGCGGGCAGGATCGTAGTCGTAAc                                                                                                                                                                        <  1:932783/251‑1 (MQ=255)

                                                                           ggctcaataaccccccccagtctCACCGCCACCAACGGTGGCAGACACAGCAACAGCGCCAGCACAAGTAACCCCGTGCCGCAAATGCGGCTATGCAGGCGATCTTCCGGGTCGCGCTAGCCTTGCAGCAGCGTGGTGCCTGGCGCAATGGCCTTACTCAATCGCTGACTCAGCAGCACCAACCCGAGGCAACACACCTGCTGGATCA‑GCGCCAGCATCGCCGCGCGGGCAGGATCGTAGTCGTAACTCAg                                                                                                                                                                    <  2:157538/229‑1 (MQ=255)

                                                                            ggcgtttcacccctcccaacctCACCGCCAGCAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCTGGCGCAATGGCCTTACTCAATCGCTGACTCAGCAGCACCAACCCGAGGCAACACACCATCTGGATCA‑GCGCCAGCATCGCCGCGCGGGCAGGATCGTAGTCGTAACTCAgc                                                                                                                                                                   <  2:893760/230‑1 (MQ=255)

                                                                               gATTTAGCCCATCGACGATCACCGCCAGCAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCTGGCGCAATGGCCTTACTCAATCGCTGACTCAGCAGCACCAACCCGAGGCAACACACCATCTGGATCA‑GCGCCAGCATCGCCGCGCGGGCAGGATCGTAGTCGTAACTCAGCGcc                                                                                                                                                                >  1:858674/1‑251 (MQ=255)

                                                                                atttagccaatctacgtccACCGCCAGCAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCTGGCGCAATGGCCTTACTCAATCGCTGACTCAGCAGCACCAACCCGAGGCAACACACCATCTGGATCA‑GCGCCAGCATCGCCGCGCGGGCAGGATCGTAGTCGTAACTCAGCGCCt                                                                                                                                                               <  1:536348/233‑1 (MQ=255)

                                                                                      cccATCGACGATCACCGCCAGCAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCTGGCGCAATGGCCTTACTCAATCGCTGACTCAGCAGCACCAACCCGAGGCAACACACCATCTGGATCA‑GCGCCAGCATCGCCGCGCGGGCAGGATCGTAGTCGTAACTCAGCGCCTGATAGa                                                                                                                                                         <  1:42216/251‑1 (MQ=255)

                                                                                        cacggccacccACCGCCAGCAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGAGCCTGGCGCAATGGCCTTACTCAATCGCTGACTCAGCAGCACCAACCCGAGGCAACACACCATCTGGATCA‑GCGCCAGCATCGCCGCGCGGGCAGGATCGTAGTCGTAACTCAGCGCCTGATAGAtt                                                                                                                                                       <  2:706813/241‑1 (MQ=255)

                                                                                        cATCGACGATCACCGCCAGCAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCTGGCGCAATGGCCTTACTCAATCGCTGACTCAGCAGCACCAACCCGAGGCAACACACCATCTGGATCA‑GCGCCAGCATCGCCGCGCGGGCAGGATCGTAGTCGTAACTCAGCGCCTGATAGAtt                                                                                                                                                       >  1:434128/1‑251 (MQ=255)

                                                                                         aTCGATGATCACCGCCAGCAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTCTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCACCAGCGTGGTGCCTGGCGCAATGGCCTTACTCCATCGCTGACTCAGCAGCACCAACCCGAGGCAACACACCATCTGGATCA‑GCGCCCGCATCGCCGCGCGGGCAGGATCGTAGTCGTACCCCAGAGCCTGATAGATTg                                                                                                                                                      >  2:402159/1‑251 (MQ=255)

                                                                                         aTCGACGATCACCGCCAGCAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCTGGCGCAATGGCCTTACTCAATCGCTGACTCAGCAGCACCAACCCGAGGCAACACACCATCTGGATCA‑GCGCCAGCATCGCCGCGCGGGCAGGATCGTAGTCGTAACTCAGCGCCTGATAGATTg                                                                                                                                                      >  1:569713/1‑251 (MQ=255)

                                                                                           cgacgaCCACCGCCAGCAACGGTGGCAGCAACGGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCTGGCGCAATGGCCTTACTCAATCGCTGACTCAGCAGCACCAACCCGAGGCAACACACCATCTGGATCA‑GCGCCAGCATCGCCGCGCGGGCAGGATCGTAGTCGTAACTCAGCGCCTGATAGATTGcc                                                                                                                                                    <  1:665676/251‑1 (MQ=255)

                                                                                            gacgaTCACCGCCAGCAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCTGGCGCAATGGCCTTACTCAATCGCTGACTCAGCAGCACCAACCCGAGGCAACACACCATCTGGATCA‑GCGCCAGCATCGCCGCGCGGGCAGGATCGTAGTCGTAACTCAGCGCCTGATAGATTGcc                                                                                                                                                    >  1:464379/1‑250 (MQ=255)

                                                                                              cgaTCACCGCCAGCAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCTGGCGCAATGGCCTTACTCAATCGCTGACTCAGCAGCACCAACCCGAGGCAACACACCATCTGGATCA‑GCGCCAGCATCGCCGCGCGGGCAGGATCGTAGTCGTAACTCAGCGCCTGATAGATTGCCAGc                                                                                                                                                 >  1:811289/1‑251 (MQ=255)

                                                                                               gaccacctccagcatcGGTGGCAGCAACAGCAGCAGCGCCAGCACAAGTAACCCTGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGAGTGGTGCCTGGCGCAATGGCCTGACGCAATCGCTGACTCAGCAGCACCAACCCGAGGCAACACACCATCTGGATCA‑GCGCCAGCATCGCCGCGCGGGCAGGATCGTAGTCGTAACTCATCGCCTGATAGATTGCCAGCt                                                                                                                                                <  2:791726/236‑1 (MQ=255)

                                                                                               gaTCACCGCCAGCAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCTGGCGCAATGGCCTTACTCAATCGCTGACTCAGCAGCACCAACCCGAGGCAACACACCATCTGGATCA‑GCGCCAGCATCGCCGCGCGGGCAGGATCGTAGTCGTAACTCAGCGCCTGATAGATTGCCAGCt                                                                                                                                                >  1:409018/1‑251 (MQ=255)

                                                                                                      accAGCAACGATGGCAGCAACAGCAGCAGCGCAAGCACAATTAAAAACGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGGCGCGCCAGCCTTGCAACAGCGTGGTGCCTGACGCAATGGCCTTACTACATCGCTGACTCAGCAGACCCAACCCAAGGCAACACACCATCTGAATCA‑GCGCCAGCATCGACGCGCGGGCCGGATCGTAGTCGTCACTCAGCTCCTGATCGATTgaccgctgggtagg                                                                                                                                         >  2:187229/2‑238 (MQ=255)

                                                                                                               ggTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCTGGCGCACTGGCCTTACTCAATCGCTGACTCCGCAGCACCAACCCGAGGCAACACACCATCTGGATCA‑GCGCCAGCATCGCCGCGAGGGCAGGATCGAAGTCGTAACTAAGCGCCTGATAGAGTGCCAGCTAGGAAGTGGTTGCCTg                                                                                                                                >  2:121873/1‑251 (MQ=255)

                                                                                                                gTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCTGGCGCAATGGCCTTACTCAATCGCTGACTCAGCAGCACCAACCCGAGGCAACACACCATCTGGATCA‑GCGCCAGCATCGCCGCGCGGGCAGGATCGTAGTCGTAACTCAGCGCCTGATAGATTGCCAGCTCGATAGTGGTTGCCTGc                                                                                                                               >  1:96920/1‑251 (MQ=255)

                                                                                                                    cagcaACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCTGGCGCAATGGCCTTACTCAATCGCTGACTCAGCAGCACCAACCCGAGGCAACACACCATCTGGATCA‑GCGCCAGCATCGCCGCGCGGGCAGGATCGTAGTCGTAACTCAGCGCCTGATAGATTGCCAGCTCGATAGTGGTTGCCTGCGGAc                                                                                                                           <  1:389289/251‑1 (MQ=255)

                                                                                                                     agcaACAGCAGCAGCGCCAGCACAATTAACCCCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCTGGCGCAATGGCCTTACTCAATCGCTGACTCAGCAGCACCAACCCGAGGCAACACACCATCTGGATCA‑GCGCCAGCATCGCCGCGCGGGCAGGATCGTAGTCGTAACTCAGCGCCTGATAGATTGCCAGCTCGATAGTGGTTGCCTGCGGAcc                                                                                                                          <  2:410034/251‑1 (MQ=255)

                                                                                                                     agcaACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCTGGCGCAATGGCCTTACTCAATCGCTGACTCAGCAGCACCAACCCGAGGCAACACACCATCTGGATCA‑GCGCCAGCATCGCCGCGCGGGCAGGATCGTAGTCGTAACTCAGCGCCTGATAGATTGCCAGCTCGATAGTGGTTGCCTGCGGAcc                                                                                                                          <  1:371721/251‑1 (MQ=255)

                                                                                                                      aaaacaggtgcagcGCCAGCAAAATTAACACCGTGTCGCAAATGCGGCAATGCAGACGCTCTTCCGGCTCACGCCAGCCTTGCAGACGCGTGGTGCCTGGCGCAATGGCCTTACACAATCGCTCACTCAGCAGCACCAACCCAAGGCTACACTCCATCTGGATCA‑GCGCCAGCATCGCCGCGCGGGCAGTATCGTAGTCGTAACGCAGCGCCTGATAGATTGCCAGCTCGATAGTGTTTGCCTGCTGACCg                                                                                                                         <  2:443089/242‑1 (MQ=255)

                                                                                                                             cagcagcGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCTGGCGCAATGGCCTTACTCAATCGCTGACTCAGCAGCACCAACCCGAGGCAACACACCATCTGGAGCA‑GCGACAGCATCGCCGCGCGGGAAGGAGCGTAGGCGTAACGCAGCGCCGGATAGACTGCCAGCACGACAGTGGAGGCCTGCGGACCgacgcacc                                                                                                                  >  2:398633/1‑244 (MQ=255)

                                                                                                                                cagcGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCTGGCGCAATGGCCTTACTCAATCGCTGACTCAGCAGCACCAACCCGAGGCAACACACCATCTGGATCA‑GCGCCAGCATCGCCGCGCGGGCAGGATCGTAGTCGTAACTCAGCGCCTGATAGATTGCCAGCTCGATAGTGGTTGCCTGCGGACCGCCGCCCAGCg                                                                                                               <  1:376599/251‑1 (MQ=255)

                                                                                                                                cacggcacgCACAATTAACACCGGGCCGCAACTGCGCATATGAATACGATCGCCCGGGTCGCTCCGGCCTTGCCGACGCGTGGGGACAGCCGCAATGGCCTTACCCAATCGGTTACTCAGCAGCACCATCCCGAGCCAACACACCATCTTGATCA‑GCGCCAGCATCGCCGCGCGGGCAGGATCGTAGTCGTAACTCAGCGCCGGATAGATTGCCAGCTCGATAGTGGTTGCCTGCGGACCGCCGCCCAGCg                                                                                                               <  2:807414/243‑1 (MQ=255)

                                                                                                                                 agcGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGCCGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCTGGCGCAATGGCCTTACTCAATCGCTGACTCAGCAGCCCCAACCCGAGGCAACACACCATCTGGATCA‑GCGCCCGCATCGCCGCGCGGGCAGGATCGTCGTCGTAACCCAGCGCCTGCTAGATTGCCAGCTCGTTAGTGGTTGCCTGCGGACACccccccgaca                                                                                                               >  2:358905/1‑246 (MQ=255)

                                                                                                                                 agcGCCAGCACAATTAACACCGTGGCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCTGGCGCAATGGCCTTACTCAATCGCTGACTCAGCAGCACCAACCCGAGGCAACACACCATCTGGATCA‑gcg                                                                                                                                                                                                            <  2:814990/157‑1 (MQ=255)

                                                                                                                                        gCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGTCGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCTGGCGCAATGGCCTTACTCAATCGCTGACTCAGCAGCACCAACCCGAGGCAACACCCCATCTGGATCA‑GCGCCAGCATCGCCGCGCGGGCAGGATCGTAGTCGTAACTCAGCGCCTGATAGATTGCCAGCTCGATAGTGGTTGCCTGCGGACCGCCGCCCAGCGATAGCACg                                                                                                       <  2:263189/251‑1 (MQ=255)

                                                                                                                                           caATTACCACCGTGTCGCAAATGCGGCTATGCAGACGATCGGCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCTGGCGCAATGGCCTTACTCAATCGCTGACTCAGCAGCACCAACCCGAGGCAACACACCATCTGGATCA‑GCGCCAGCATCGCCGCGCGGGCAGGATCGTAGTCGTAACTCAGCGCCTGATAGATTGCCAGCTCGATAGTGGTTGCCTGCGGACCGCCGCCCAGCGATAGCACGGTg                                                                                                    <  2:984655/251‑1 (MQ=255)

                                                                                                                                              ttAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCTGGCGCAATGGCCTTACTCAATCGCTGACTCAGCAGCACCAACCCGAGGCAACACACCATCTGGATCA‑GCGCCAGCATCGCCGCGCGGGCAGGATCGTAGTCGTAACTCAGCGCCTGATAGATTGCCAGCTCGATAGTGGTTGCCTGCGGACCGCCGCCCAGCGATAGCACGGTgacg                                                                                                 >  1:482847/1‑248 (MQ=255)

                                                                                                                                               tAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCTGGCGCAATGGCCTTACTCAATCGCTGACTCAGCAGCACCAACCCGAGGCAACACACCATCTGGATCA‑GCGCCAGCATCGCCGCGCGGGCAGGATCGTAGTCGTAACTCAGCGCCTGATAGATTGCCAGCTCGATAGTGGTTGCCTGCGGACCGCCGCCCAGCGATAGCACGGTGGCg                                                                                                 <  1:92582/250‑1 (MQ=255)

                                                                                                                                               tAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCTGGCGCAATGGCCTTACTCAATCGCTGACTCAGCAGCACCAACCCGAGGCAACACACCATCTGGATCA‑GCGCCAGCATCGCCGCGCGGGCAGGATCGTAGTCGTAACTCAGCGCCTGATAGATTGCCAGCTCGATAGTGGTTGCCTGCGGACCGCCGCCCAGCGATAGCACGGTGGCg                                                                                                 >  1:139514/1‑250 (MQ=255)

                                                                                                                                               tAACACCGTGTCGCAAAGGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCTGGCGCAATGGCCTTACTCAATCGCTGACTCAGCAGCACCAACCCGAGGCAACACACCACCTGGATCA‑GCGCCAGCATCGCCGCGCGGGCAGGATCGTAGTCGTAACTCAGCGCCTGATAGATTGCCAGCTCGATAGTGGTTGCCTGCGGACCGCCGCCCAGCGATAGCACGGTGGCg                                                                                                 <  2:577473/250‑1 (MQ=255)

                                                                                                                                                aaCACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCTGGCGCAATGGCCTTACTCAATCGCTGACTCAGCAGCACCAACCCGAGGCAACACACCATCTGGATCA‑GCGCCAGCATCGCCGCGCGGGCAGGATCGTAGTCGTAACTCAGCGCCTGATAGATTGCCAGCTCGATAGTGGTTGCCTGCGGACCGCCGCCCAGCGATAGCACGGTGGCGaa                                                                                               <  1:76892/251‑1 (MQ=255)

                                                                                                                                                 acacCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCTGGCGCAATGGCCTTACTCAATCGCTGACTCAGCAGCACCAACCCGAGGCAACACACCATCTGGATCA‑GCGCCAGCATCGCCGCGCGGGCAGGATCGTAGTCGTAACTCAGCGCCTGATAGATTGCCAGCTCGATAGTGGTTGCCTGCGGACCGCCGCCCAGCGATAGCACGGTGGCGAAg                                                                                              <  2:452417/251‑1 (MQ=255)

                                                                                                                                                   cccGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCTGGCGCAATGGCCTTACTCAATCGCTGACTCAGCAGCACCAACCCGAGGCAACACACCATCTGGATCA‑GCGCCAGCATCGCCGCGCGGGCAGGATCGTAGTCGTAACTCAGCGCCTGATAGATTGCCAGCTCGATAGTGGTTGCCTGCGGACCGCCGCCCAGCGATAGCACGGTGGCGAAGCt                                                                                            <  1:483107/250‑1 (MQ=255)

                                                                                                                                                   acCGTGTCACAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCTGGCGCAATGGCCTTACTCAATCGCTGACTCAGCAGCACCAACCCGAGGCAACACACCATCTGGATCA‑GCGCCAGCATCGCCGCGCGGGCAGGATCGTAGTCGTAACTCAGCGCCTGATAGATCGCAAGCTCGATAGTGGTTGCCTGCGGACCGCCGCCCAGCGATAGCACGGCGGCGAAGCt                                                                                            >  1:617/1‑251 (MQ=255)

                                                                                                                                                    ccGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCTGGCGCAATGGCCTTACTCAATCGCTGACTCAGCAGCACCAACCCGAGGCAACACACCATCTGGATCA‑GCGCCAGCATCGCCGCGCGGGCAGGATCGTAGTCGTAACTCAGCGCCTGATAGATTGCCAGCTCGATAGTGGTTGCCTGCGGACCGCCGCCCAGCGATAGCACGGTGGCGAAGCTg                                                                                           <  2:273302/251‑1 (MQ=255)

                                                                                                                                                        gtCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCTGGCGCAATGGCCTTACTCAATCGCTGACTCAGCAGCACCAACCCGAGGCAACACACCATCTGGATCA‑GCGCCAGCATCGCCGCGCGGGCAGGATCGTAGTCGTAACTCAGCGCCTGATAGATTGCCAGCTCGATAGTGGTTGCCTGCGGACCGCCGCCCAGCGATAGCACGGTGGCGAAGCTGGCGa                                                                                       <  1:26516/251‑1 (MQ=255)

                                                                                                                                                           gCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCTGGCGCAATGGCCTTACTCAATCGCTGACTCAGCAGCACCAACCCGAGGCAACACACCATCTGGATCA‑GCGCCAGCATCGCCGCGCGGGCAGGATCGTAGTCGTAACTCAGCGCCTGATAGGTTGCCAGCTCGATAGTGGTTGCCTGCGGACCGCCGCCCAGCGATAGCACGGTGGCGAAGCTGGCGaaa                                                                                     >  1:178774/1‑250 (MQ=255)

                                                                                                                                                               aTGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCTGGCGCAATGGCCTTACTCAATCGCTGACTCAGCAGCACCAACCCGAGGCAACACACCATCTGGATCA‑GCGCCAGCATCGCCGCGCGGGCAGGATCGTAGTCGTAACTCAGCGCCTGATAGATTGCCAGCTCGATAGTGGTTGCCTGCGGCCCGCCGCCCAGCGATAGCAAGGGTGCGAAGCTGGCGAGACag                                                                                  >  2:730117/1‑249 (MQ=255)

                                                                                                                                                                  cGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCTGGCGCAATGGCCTTACTCAATCGCTGACTCAGCAGCACCAACCCGAGGCAACACACCATCTGGATCA‑GCGCCAGCATCGCCGCGCGGGCAGGATCGGAGACGTAACTCAGCGCCTGATAGATTGCCAGCTCGATAGTGGTTGCCTGCGGACCGCCGCCCAGCGATAGCACGGTGGCGAAGCTGGCGAAACAGAGCAt                                                                             >  1:828099/1‑251 (MQ=255)

                                                                                                                                                                   ggCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCTGGCGCAATGGCCTTACTCAATCGCTGACTCAGCAGCACCAACCCGAGGCAACACACCATCTGGATCA‑GCGCCAGCATCGCCGCGCGGGCAGGATCGTAGTCGTAACTCAGCGCCTGATAGATTGCCAGCTCGATAGTGGTTGCCTGCGGACCGCCGCCCAGCGATAGCACGGTGGCGCAGCTGGCGAAACAGAGCAt                                                                             >  1:150563/1‑250 (MQ=255)

                                                                                                                                                                    gCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCTGGCGCAATGGCCTTACTCAATCGCGGACTCAGCAGCACCAACCCGAGGCAACACACCATCTGGATCA‑GCGCCAGCATCGCCGCGCGGGCAGGATCGTAGTCGTAACTCAGCGCCTGATAGATTGCCAGCTCGATAGTGGTTGCCTGCGGACCGCCGCCCAGCGATAGCACGGTGGCGAAGCTGGCGAAACAGAGCATaa                                                                           <  2:838403/251‑1 (MQ=255)

                                                                                                                                                                      tATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCTGGCGCAATGGCCTTACTCAATCGCTGACTCAGCAGCACCAACCCGAGGCAACACACCAACTGGATCA‑GCGCCAGCATCGCCGCGCGGGCAGGATCGTAGTCGTAACTCAGCGCCTGATAGATTGCCAGCTCGATAGTGGTTGCCTGCGGCCCGCCGCCCAGCGATAGCACGGTGGCGAAGATGGAGAAACAGAGCCTaa                                                                           >  1:475455/1‑249 (MQ=255)

                                                                                                                                                                         gCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCTGGCGCAATGGCCTTACTCAATCGCTGACTCAGCAGCACCAACCCGAGGCAACACACCATCTGGATCA‑GCGCCATCATCGCCGCGCGGGCAGGATCGTAGTCGTAACTCAGCGCCTGATAGATTGCCAGCTCGATAGTGGTTGCCTGCGGACCGCCGCCCAGCGATAGCACGGTGGCGAAGCTGGCGAAACAGAGCATAAAGATa                                                                      <  1:761361/251‑1 (MQ=255)

                                                                                                                                                                         gCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCTGGCGCAATGGCCTTACTCAATCGCTGACTCAGCAGCACCAACCCGAGGCAACACACCATCTGGATCA‑GCGCCAGCATCGCCGCGCGGGCAGGATCGTAGTCGTAACTCAGCGCCTGATAGATTGCCAGCTCGATAGTGGTTGCCTGCGGACCGCCGCCCAGCGATAGCACGGTGGCGAAGCTGGCGAAACAGAGCATAAAGATa                                                                      <  1:416954/251‑1 (MQ=255)

                                                                                                                                                                            gACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCTGGCGCAATGGCCTTACTCAATCGCTGACTCAGCAGCACCAACCCGAGGCAACACACCATCTGGATCA‑GCGCCAGCATCGCCGCGCGGGCAGGATCGTAGTCGTAACTCAGCGCCTGATAGATTGCCAGCTCGATAGTGGTTGCCTGCGGACCGCCGCCCAGCGATAGCACGGTGGCGAAGCTGGCGAAACAGAGCATAAAGATA‑Agt                                                                  <  1:313888/251‑2 (MQ=255)

                                                                                                                                                                                  cGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCTGGCGCAATGGCCTTACTCAATCGCTGACTCAGCAGCACCAACCCGAGGCAACACACCATCTGGATCA‑GCGCCAGCATCGCCGCGCGGGCAGGATCGTAGTCGTAACTCAGCGCCTGATAGATTGCCAGCTCGATAGTGGTTGCCTGCGGACCGCCGCCCAGCGATAGCACGGTGGCGAAGCTGGCGAAACAGAGCATAAAGATA‑AGTgcagca                                                            <  1:931435/251‑1 (MQ=255)

                                                                                                                                                                                     ccGGGTCGCGCCAGCCTTGCAGCCGCTTGGTGCCTGGCGCAATGGCCTTACTCAATCGCTGACTCAGCAGCACCAACCCGAGGCAACACTCCAGCTGGATCA‑GCGCCAGCATCGCCGCGCGGGCAGGATCGTAGTCGTAACTCAGCGCCTGATAGATTGCCAGCTCGATAGTGGTTGCCTGCGGACCGCCGCCCAGCGATAGCACGGTGGCGAAGCTGGCGAAACAGAGCATAAAGATA‑AGTGCAGCAAc                                                          <  2:575363/250‑1 (MQ=255)

                                                                                                                                                                                     ccGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCTGGCGCAATGGCCTTACTCAATCGCTGACTCAGCAGCACCAACCCGAGGCAACACACCATCTGGATCA‑GCGCCAGCATCGCCGCGCGGGCAGGATCGTAGTCGTAACTCAGCGCCTGATAGATTGCCAGCTCGATAGTGGTTGCCTGCGGACCGCCGCCCAGCGATAGCACGGTGGCGAAGCTGGCGAAACAGAGCATAAAGATA‑AGTGCAGCAAc                                                          <  1:45197/250‑1 (MQ=255)

                                                                                                                                                                                      cGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCTGGCGCAATGGCCTTACTCAATCGCTGACTCAGCAGCACCAACCCGAGGCAACACACCATCTGGATCA‑GCGCCAGCATCGCTGCGCGGGCAGGATCGTAGTCGTAACTCAGCGACTGATAGATTGCCAGCTCGATAGTGGTTGCCTGCGGACCGCCGCCCAGCGCTAGCACGGTGGCGAAGCTGGCGACACAGAGCATAAAGATA‑AGTGCAGCAACcg                                                        >  1:636149/1‑251 (MQ=255)

                                                                                                                                                                                      cGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCTGGCGCAATGGCCTTACTCAATCGCTGACTCAGCAGCACCAACCCGAGGCAACACACCATCTGGATCA‑GCGCCAGCATCGCCGCGCGGGCAGGATCGTAGTCGTAACTCAGCGCCTGATAGATTGCCAGCTAGATAGTGGTTGCATGCGGACCGCCGCCCAGCGATAGCACGGTGGCGAAGCTGGCGAAACAGAGCATGAAGATA‑AGTGCAGCAACcg                                                        >  1:861808/1‑251 (MQ=255)

                                                                                                                                                                                         gTCGCGCCAGCCTTGCAGCAGCGGGGTGCCTGGCGCAATGGCCTTACTCAATCGCTGACTCAGCAGCACCAACCCGAGGCAACACACCATCTGGATCA‑GCGCCAGCATCGCCGCGCGGGCAGGATCGTAGTCGTAACTCAGCGCCTGATAGATTGCCAGCTCGATAGTGGTTGCCTGCGGACCGCCGCCCAGCGATAGCACGGTGGCGAAGCTGGCGAAACAGAGCATAAAGATA‑AGTGCAGCAACcggcg                                                     <  1:406638/251‑1 (MQ=255)

                                                                                                                                                                                          ccgcgCCAGCCTTGAAGCAGCGTGGTGCCTGGCGCTATGGCCTTACTCAATCGCTGACTGATCAGCACCAACCCTAGGCAACACACCATCTGGATCA‑GCGCCAGCATCGCCGCGCGGGCAGGATCGTAGTCGTAACTCAGCGCCTGATAGAGTGCCAGCTCGATAGTGGTTGCCTGCGGACCGCCGCCCAGCGATAGCACGGGGGCGAAGCTGGTGAAACAGAGCAGAAAGATA‑AGTGCGGCACCcggcgg                                                    <  2:489982/250‑1 (MQ=255)

                                                                                                                                                                                              gcCAGCCTTGCAGCAGCGTGGTGCCTGGCGCAATGGCCTTACTCAATCGCTGACTCAGCAGCACCAACCCGAGGCAACACACCATCTGGATCA‑GCGCCAGCATCGCCGCGCGGGCAGGATCGTAGTCGTAACTCAGCGCCTGATAGATTGCCAGCTCGATAGTGGTTGCCTGCGGACCGCCGCCCAGCGATAGCACGGTGGCGAAGCTGGCGAAACAGAGCATAAAGATA‑AGTGCAGCAACCGGCGGGAtt                                                >  1:196406/1‑251 (MQ=255)

                                                                                                                                                                                               ccAGCCTTGCAGCAGCGTGGTGCCTGGCGCAATGGCCTTACTCAAGCGCTGACTCAGCAGCACCAACCCGAGGCAACACTCCATCTGGATCA‑GCGCCAGCATCGCCGCGCGGGCAGGATCGTAGTCGTAACTCAGCGCCTGATAGATTGCCAGCTCGATAGTGGTTGCCTGCGGACCGCCGCCCAGCGATAGCACGGTGGCGAAGCTGGCGAAACAGAGCATAAAGATA‑AGTGCAGCAACCGGCGGGAttt                                               <  1:707355/251‑1 (MQ=255)

                                                                                                                                                                                                cAGCCTTGCAGCAGCGTGGTGCCTGGCGCAATGGCCTTACTCAATCGCTGACTCAGCAGCACCAACCCGAGGCAACACACCATCTGGATCA‑GCGCCAGCATCGCCGCGCGGGCAGGATCGTAGTCGTAACTCAGCGCCTGATAGATTGCCAGCTCGATAGTGGTTGCCTGCGGACCGCCGCCCAGCGATAGCACGGTGGCGAAGCTGGCGAAACAGAGCATAAAGATA‑AGTGCAGCAACCGGCGGGATTTg                                              <  1:803040/251‑1 (MQ=255)

                                                                                                                                                                                                cAGCCTTGCAGCAGCGTGGTGCCTGGCGCAATGGCCTTACTCAATCGCTGACTCAGCAGCACCAACCCGAGGCAACACACCATCTGGATCA‑GCGCCAGCATCGCCGCGCGGGCAGGATCGTAGTCGTAACTCAGCGCCTGATAGATTGCCAGCTCGATAGTGGTTGCCTGCGGACCGCCGCCCAGCGATAGCACGGTGGCGAAGCTGGCGAAACAGAGCATAAAGATA‑AGTGCAGCAACCGGCGGGATTTg                                              <  1:77489/251‑1 (MQ=255)

                                                                                                                                                                                                 aGCCTTGCAGCAGCGTGGTGCCTGGCGCAATGGCCTTACTCAATCGCTGACTCAGCAGCACCAACCCGAGGCAACACACCATCTGGATCA‑GCGCCAGCATCGCCGCGCGGGCAGGATCGTAGTCGTAACTCAGCGCCTGATAGATTGCCAGCTCGATAGTGGTTGCCTGCGGACCGCCGCCCAGCGATAGCACGGTGGCGAAGCTGGCGAAACAGAGCATAAAGATA‑AGTGCAGCAACCGGCGGGATTTGt                                             <  1:750522/251‑1 (MQ=255)

                                                                                                                                                                                                    cTTGCAGCAGCGTGGTGCCTGGCGCAATGGCCTTAATCAATCGCTGACTCAGCAGCACCAACCCGACGCAACACACCATCCGGATCA‑GCGCCAGCATAGCCGCGCGGGCAGGATCGTAGTCGTAACTCAGCGCCTGATAGATTGCCAGCTCGCTAGTGGTTGCCTGCGGACCGCCGCCCAGCGATAGCACGGTGGCGAGGCTGGCGAAACAGAGCATAAAGATACAG‑GCAGCAACCGGCGGGAATTGtacc                                          >  2:941766/1‑248 (MQ=255)

                                                                                                                                                                                                       gcagcaGCGTGGTGCCTGGCGCAATGGCCTTACTCAATCGCTGACTCAGCAGCACCAACCCGAGGCAACACACCATCTGGATCA‑GCGCCAGCATCGCCGCGCGGGCAGGATCGTAGTCGTAACTCAGCGCCTGATAGATTGCCAGCTCGATAGTGGTTGCCTGCGGACCGCCGCCCAGCGATAGCACGGTGGCGAAGCTGGCGAAACAGAGCATAAAGATA‑AGTGCAGCAACCGGCGGGAGTTGTCGCCg                                        <  1:161854/250‑1 (MQ=255)

                                                                                                                                                                                                       gcagcaGCGTGGTGCCGGGCGCAATGGCCTTACTCAATCGCTGACTCAACAGCACCAGCCCGAGGCAGCACACCATCTGGAGCA‑GCGCCAGCATTGCCGCGCGGGCAGGATCGTAGTCGTAACTCAGCGCCTGATAGATTGCCAGCTCGATAGTGGTCGCCTGCGGACCGCCCCCCAGCGATAGCACGGTGGCGAAGCTGGCGAAACAGAGCATAAAGATA‑AGCGCAGCAACCGGCGGGATTTGTCGCCgg                                       >  1:469714/1‑250 (MQ=255)

                                                                                                                                                                                                             gcGTGGTGCCGGGCGCAATGGCCTTACTCAATCGCTGACTCAACAGCACCAGCCCGAGGCAGCACACCATCTGGAGCA‑GCGCCAGCATTGCCGCGCGGGCAGGATCGTAGTCGTAACTCAGCGCCTGATAGATTGCCAGCTCGATAGTGGTCGCCTGCGGACCGCCCCCCAGCGATAGCACGGTGGCGAAGCTGGCGAAACAGAGCATAAAGATA‑AGCGCAGCAACCGGCGGGATTTGTCGCCGTAACCAc                                 <  1:336811/251‑1 (MQ=255)

                                                                                                                                                                                                                 ggtgCCTGGCGCAATGGCCTTACTCAATCGCTGACTCAGCAGCACCAACCCGAGGCAACACACCATCTGGATCA‑GCGCCAGCATCGCCGCGCGGGCAGGATCGTAGTCGTAACTCAGCGCCTGATAGATTGCCAGCTCGATAGTGGTTGCCTGCGGACCGCCGCCCAGCGATAGCACGGTGGCGAAGCTGGCGAAACAGAGCATAAAGATA‑AGTGCAGCAACCGGCGGGATTTGTCGCCGTAACCACGGcc                             >  1:265988/1‑251 (MQ=255)

                                                                                                                                                                                                                    gcctggCGCAATGGCCTTACTCAAACGCTGACCCAGCAGCACCAACCCGAGGCAACACGCCATCTGGACCA‑GCGCCAGCATCGCCGCGCGGGCAGGATCGTAGTCGTAACCCAGCGCCTGATAGATTGCCAGCTCGATAGTGGTTGCCGGCGGACCGCCGCCCAGCGATAGCACGGTGGCGAAGCTGGCGAAACAGAGCATAAAGATA‑AGTGCAGCAACCGGCGGGATTTGTCGCCGTAACCACGGcaat                           <  2:302197/246‑4 (MQ=255)

                                                                                                                                                                                                                    gcctggCGCAATGGCCTTACGCAATCTCTGACTCAGCAGCACCAACCCGAGGCAACACCCCATCTGGATCA‑GCGCCAGCATCGCCGCGCGGGCAGGATCGTAGTCGTAACTCAGCGCCTGATAGATTGCCCGCTCGATAGTGGTTGCCTGCGGACCGCCGCCCAGCGATAGCACGGTGGCGAAGCTGGCGAAACAGAGCATAAAGATA‑AGTGCAGCAACCGGCGGGATTTGTCGCCGTACCCACGGCCAtt                          <  2:504418/247‑1 (MQ=255)

                                                                                                                                                                                                                     cctgtcccAATGGCCTTACTCAATCCCTGACTCAGCAGCACCAACCCGAGGCAACACCCCATCTGGACCA‑GCGCCAGCATCGCCGCGCGGGCAGGATCGTAGTCGTAACTCAGCGCCTGATAGATTGCCAGCTCGATAGTGGTTGCCTGCGGACCGCCGCCCAGCGATAGCACGGTGGCGAAGCTGGCGAAACAGAGCATAAAGATA‑AGTGCAGCAACCGGCGGGATTTGTCGCCGTAACCACGGCCATTc                         <  2:346974/244‑1 (MQ=255)

                                                                                                                                                                                                                        ggCGAAATGCCCTAACTCCATCCCTGACTCAGCAGCACCAACCCGAGGCAACCCTCCATCCGGATCA‑GCGCCAGCATCGCCGCGCGGGCAGGAGCGTAGGCGTAACTCAGCGCCTGATAGAATGCCAGCCCGATAGTGGTTGCCTGCGGACCGCCGCCCAGCGATAGCACGGTGGCGAAGCTGGCGAAACAGAGCATAAAGATA‑AGTGCAGCAACCGGCGGGATTTGTCGCCGTAACCACGGCCATTcgac                      <  2:279886/251‑1 (MQ=255)

                                                                                                                                                                                                                           gcAATGGCCTTACTCAATCGCTGACTCAGCAGCACCAACCCGAGGCAACACACCATCTGGATCA‑GCGCCAGCATCGCCGCGCGGGCAGGATCGTAGTCGTAACTCAGCGCCTGATAGATTGCCAGCTCGATAGTGGTTGCCTGCGGACCGCCGCCCAGCGATAGCACGGTGGCGAAGCTGGCGAAACAGAGCATAAAGATA‑AGTGCAGCAACCGGCGGGATTTGTCGCCGTAACCACGGCCATTCGACGaa                   <  1:290168/251‑1 (MQ=255)

                                                                                                                                                                                                                                  ccTTACTCAAGCGCGGACTCAGCAGCACCAACCCGAGGCAACACACCATCTGGATCA‑GCGCCAGCATCGCCGCGCGGGCAGGATCGTAGTCGTAACTCAGCGCCTGATAGATTGCCAGCTCGATAGTGGTTGCCTGCGGACCGCCGCCCAGCGATAGCACGGTGGCGAAGCTGGCGAAACAGAGCATAAAGATA‑AGTGCAGCAACCGGCGGGATTTGTCGCCGTAACCACGGCCATTCGACGAAGCGGaaa            <  2:902546/251‑1 (MQ=255)

                                                                                                                                                                                                                                         caaccGCTGACTCAGCAGCACCAACCCGAGGCAACACACCATCTGGATCA‑GCGCCAGCATCGCCGCGCGGGCAGGATCGTAGTCGTAACTCAGCGCCTGATAGATGGCCAGCTCGATAGTGGTTGCCTGCGGACCGCCGCTCAGCGATAGCACGGTGGCGAAGCTGGCGAAACAGAGCATAAAGATA‑AGTGCAGCAACCGGCGGGATTTGTCGCCGTAACCACGGCCATTCGACGAAGCGGAAAAAATg       <  2:94273/245‑1 (MQ=255)

                                                                                                                                                                                                                                         cAATCGCTGACGCAGCAGCACCAATCCCAGGCATCACACCATCTGGTACA‑GCGCCAGCATCGCCGCGCGGGCAGGATCGTAGGCGTAACGCAGCGCCTGATAGATTGCCAGCTCGATAGTGGTTGCCTGCGGAGCGCCGCCCAGCGATAGCACGGTGGCGAAGCTGGCGAAACAGAGCATAAAGATA‑AGTGCAGCAAACGGTGGGATTGGTCGCCGTAACCACGGCCATTCGACGAAGCTGAAAAAATGcc     <  2:325941/251‑1 (MQ=255)

CCTGCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTACCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCGGGCGCAATGGCCTTACTCAATCGCTGACTCAACAGCACCAGCCCGAGGCAGCACACCATCTGGAGCA‑GCGCCAGCATTGCCGCGCGGGCAGGATCGTAGTCGTAACTCAGCGCCTGATAGATTGCCAGCTCGATAGTGGTCGCCTGCGGACCGCCCCCCAGCGATAGCACGGTGGCGAAGCTGGCGAAACAGAGCATAAAGATA‑AGCGCAGCAACCGGCGGGATTTGTCGCCGTAACCACGGCCATTCGACGAAGCGGAAAAAATGCCAGC  >  NC_000913/73497‑73983

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`