Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 73,445 C→A L359L (CTG→CTT)  thiP ← thiamine ABC transporter membrane subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091373,4450CA94.2% 257.7 / ‑5.1 105L359L (CTG→CTT) thiPthiamine ABC transporter membrane subunit
Reads supporting (aligned to +/- strand):  ref base C (6/0);  new base A (78/20);  total (85/20)
Fisher's exact test for biased strand distribution p-value = 5.93e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 105 total aligned reads displayed.

GACTGACATAACATGCTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGCACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGTGGCAGGCCGATAGTGTTG‑TTGAGCAGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACATGCCGCTCATCTCCAGCA‑CCTGACCCGCCAGCATTTTCTGCCGCGCCCGCAGTTCGCGACTGCTCCATAGCA‑GCATCATGGT‑CAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACA‑GCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTACCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCC  >  NC_000913/73200‑73689
                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                       
gACTGACACAACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGCGGCAGGCCAATGGGGTTG‑TTGAGCAGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGCCTGGCGAGGCTCAACATGCCGCTCATCCCCAGCG‑CCTGACCCGCCAGCATTTTCTGCCGCGCCCGCAGTTc                                                                                                                                                                                                                                                    >  2:123502/1‑251 (MQ=255)
  cTGACACAACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGCGGCAGGCCAATGGTGTTG‑TTGAGCAGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACATGCCGCTCATCTCCAGCG‑CCTGACCCGCCAGCATTTTCTGCCGCGCCCGAAGTTcgc                                                                                                                                                                                                                                                  >  1:706813/1‑251 (MQ=255)
    gACACAACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTCCAGCGCATAAGGGACCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGCGGCAGGCCAATGGTGTTG‑TTGAGCAGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACATGCCGCTCATCTCCAGCG‑CCGGACCCGCCAGCATTTTCTGCCGCGCCCGAAGTTcgcg                                                                                                                                                                                                                                                 <  1:597264/250‑1 (MQ=255)
         aaCATTCTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTCCTTTCACCTCAAAAGGTACCCCCATTAACGCATTGGTGAAAATCACAATCCCGACAGCATATTGCGGCAGCCCAATGGTTTTT‑TGGAGCATAAAAAAAAAGCCGGTACCCCGCACAAACCCCGGCAGGGCGGGGATCAACATTCCCCTCATCTCCATCG‑CCTGACCCGCCAGCATTTTCTGCCGCGCCCGAAGTTCGCGACTGCt                                                                                                                                                                                                                                           <  1:822751/251‑1 (MQ=255)
                      gggcggGGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATACGGGCTCGCCATTCACGCATTGGTGCAACTCACAATGCCGTCAGCAGATGGCGGCAGGCCACTGGTGTTG‑TTGAGCAGTAAAAAGAAGCCGGTAGACAGCACAATCCCCGGCATGGCGAGGCTCAACATGCCGCTCATCTCCAGAG‑CCTGACCCGCCAGCCTTTTCTGCCGCGCCCGAAGTTCGCGACTGCTCCATAGCA‑Gcatc                                                                                                                                                                                                                             >  1:915363/1‑251 (MQ=255)
                      aggcggGGATATCGCGCATCGGGGTTTCCAGTACTTTCAGCGCCTATGGGGTCGCCATTAGCGAATTGGTGAAAATCACAATGCCGTCAGCAGATTGCGACAGACCAATTGTGGTG‑TTGAGCAGACAAAAGACGCCGGAAGCCAACAACATCCCCATCATGGCGAGGATCAACATGCAGCTCATCTACA‑CAGCCAGACCCTCCCGAATTTTATGCCGCGCCCGCAGTTCCAGACTGCTcacacgccccccc                                                                                                                                                                                                                              >  2:11328/2‑239 (MQ=255)
                       ggcgtggATATCGCGCATCGGGTTTTCCATTACTTTCAGGGCAAAAGTGACCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGCGCCAGGCCAAGGGGGTTT‑TTGAGCAGAAAAAAAAAGCCGGTAGCCACCAAAATCCCCGGCAGGGCGGGGAGCACCATTCCGCTCGCCTCCAGCG‑CCTCTCCCGCCAGCATTTTCTGCCGCGCCCGAAGTTCGCGACTGCTCCATAGCA‑Gcatca                                                                                                                                                                                                                            <  1:52521/245‑1 (MQ=255)
                            tGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGCGGCAGGCCAATGGTGTTG‑TTGAGCAGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACATGCCGCTCATCTCCAGCG‑CCTGACCCGCCAGCATTTTCTGCCGCGCCCGAAGTTCGCGACTGCTCCATAGCA‑GCATCATGGT‑c                                                                                                                                                                                                                      >  1:868224/1‑251 (MQ=255)
                             gtattcccgcATCGTGTTTTCAAGTCCTTTCACCGCATAAGGTATCGCCATAACCGCATGGGTGAAAATCACATGGCCGTCAGCAGATTGCGGCAGGCCAGTGGTGTTG‑TTGAGCAGTAAAAAGAAGCCGGTAGCCACCACAATCCCCGGCTGGGCGAGGATCACCATGCCGCTCATCTCCAGCG‑CCTGACCCGCCAGCATTTTCTGCCGCGCCCGAAGTTCGCGACTGCTCCATAGCA‑GCATCATGGT‑c                                                                                                                                                                                                                      <  1:27655/243‑1 (MQ=255)
                             gATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGCGGCAGGCCCATGGTGTTG‑TTGAGCAGTAAAAAGAAGCCGGTAGCCAGCACAATACCCGGCATGGCGAGGATCAACATGCCGCTCCTCTCCCGCG‑CCTCACCCCCCCGACTTTTACGCCGCGCCCCAAGTTCGCGACTGCTCCATAGCA‑GCATCTTGGT‑c                                                                                                                                                                                                                      >  2:87812/1‑250 (MQ=255)
                               tatCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGCGGCAGGCCAATGGTGTTG‑TTGAGCAGTAAAAAGAAGCCGGAAGCCAGCACAATCCCCGGCATGGCGAGGATCAACATGCCGCTCATCTCCAGCG‑CCGGACCCGCCAGCATTTTCTGCCGCGCCCCAAGTTCGCGACTGCTCCATAGCA‑GCATCATGGT‑CAGc                                                                                                                                                                                                                   >  1:513818/1‑251 (MQ=255)
                                atCGCGCATCGGGTTTTCCAGTCCTTACAGCGCATAAGGGATCGCCATTAACGCATTGGTAAAAATCACAATGCCGTCAGCAGATTGCGGCAGCCCAAGGGTGTTT‑TGGAGCAGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACATGCCGCTCATCTCCAGCG‑CCTGACCCGCCAGCATTTTCTGCCGCGCCCGAAGTTCGCGACTGCTCCATAGCA‑GCATCATGGT‑CAGCa                                                                                                                                                                                                                  <  1:419891/251‑1 (MQ=255)
                                atCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGCGGCAGGCCAATGGTGTTG‑TTGAGCAGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACATGCCGCTCATCTCCAGCG‑CCCGACCCGCCAGCATTTTCTGCCGCGCCCGAAGTTCGCGACTGCTCCATAGCA‑GCATCATGGT‑CAGc                                                                                                                                                                                                                   >  2:227205/1‑250 (MQ=255)
                                atCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGCGGCAGGCCAATGGTGTTG‑TTGAGCAGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACATGCCGCTAATCTCCAGCG‑CCTGACCCGCCAGCATTTTCTGCCGCGCCCGCAGTTCGCGACTGCTCCATAGCA‑GCATCATGGT‑AAGc                                                                                                                                                                                                                   >  1:591469/1‑250 (MQ=255)
                                 tCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGCGGCAGGCCAATGGTGTTG‑TTGAGCAGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACATGCCGCTCATCTCCAGCG‑CCTGACCCGCCAGCATTTTCTGCCGCGCCCGAAGTTCGCGACTGCTCCATAGCA‑GCATCATGGT‑CAGCAc                                                                                                                                                                                                                 >  1:43437/1‑251 (MQ=255)
                                  cgcgCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGCGGCAGGCCAATGGTGTTG‑TTGAGCAGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCACCATGCCGCTAATCTCCAGCG‑CCTGACCCGCCAGCATTTTCTGCAGCGCCCGAAGTTCGCGACTGCTCCCTAGCA‑GCAACATGGT‑CAGCACt                                                                                                                                                                                                                >  2:576261/1‑251 (MQ=255)
                                  cgcgCATCGGGATTTCCAGTACTTTCAGCGCATAAGAGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGCGGCAGGCCAATGGTGTTG‑TTGAGCAGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACATGCCGCTCATCTCCAGCG‑CCTGCCCCGCCAGCATTTTCTGCCGCCCCCGAAGTTCGCGACTGCTCCATAGCC‑GCATCATGGT‑cgccacc                                                                                                                                                                                                                >  2:371721/1‑245 (MQ=255)
                                  cgcgCACCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCTTTAACGCATGGGTAAAAACCACAAGGCCGTCAGCAGATTGCGGCAGGCCAATGGTGTTG‑TTGAGCAGAAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACATGCCGCTCATCTCCAGCG‑CCTGACCCGCCAGCATTTTCTGCCGCGCCCGAAGTTCGCGACTGCTCCATAGCA‑GCATCATGGT‑CAGCACt                                                                                                                                                                                                                <  1:529987/251‑1 (MQ=255)
                                   gcgcATCGGGTTTTCCAGTACTTTCAGCGCATACGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGCGGCAGGCCAATGGTGTTG‑TTGAGCAGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACATGCCGCTCATCTCCAGCG‑CCTGACCCGCCAGCGTTTTCTGCCGCGCCCGAAGTTCGCGACTGCTCCATAGCA‑GCATCATGGT‑CAGCACt                                                                                                                                                                                                                >  1:352341/1‑250 (MQ=255)
                                   gcgcATCGGGTTTTCCAGTACTTTCAGCGCATAAGGTATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGCGGCAGGCCACTGGTGTTG‑TTGAGCAATAAAAAGAAGCCGGTAGCCCGCACAATCCCCGGCCAGGCGAGGATCAACATGCCACTCCTCTCCCGAG‑CATGACCCTCCAGCATTTTCTGCCGCGCCCGAAGTTCGCGACCGCTCCATA‑CACGCATCATgtgccgccct                                                                                                                                                                                                                 >  2:750522/1‑241 (MQ=255)
                                    cgcATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGCGGCAGGCCAATGGTGTTG‑TTGAGCAGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACATGCCGCTCATCTCCAGCG‑CCTGACCCGCCAGCATTTTCTGCCGCGCCCGAAGTTCGCGACTGCTCCATAGCA‑GCATCATGGT‑CAGCACTAc                                                                                                                                                                                                              >  1:127998/1‑251 (MQ=255)
                                        tCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGCGGCAGGCCAATGGTGTTG‑TTGAGCAGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACATGCCGCTCATCTCCAGCG‑CCTGACCCGCCAGCATTTTCTGCCGCGCCCGAAGTTCGCGACTGCTCCATAGCA‑GCATCATGGT‑CAGCACTACGcac                                                                                                                                                                                                          >  1:410034/1‑251 (MQ=255)
                                        tCGGGTTTTCCAGAACTTTCAGAGCATAAGGGATCGCCATTAACGCATTGGTGCAAATCACAATGCCGTCAGCAGATTGCGGCAGGCCAATGGTGTTG‑TTGAGCAGTACAAAGAAGCCGGTAGCCAGCACAAGCCCCAGCATGGCGAGGATCAACATGCAGCTCATCTCCAGCG‑CCTGACCCGCGAGCATTTTCTGCCGCGCCCGAAGTTCGCGACGGATCCCTAGCA‑GCATCATGGT‑CAGCCCTACGcac                                                                                                                                                                                                          >  2:943347/1‑251 (MQ=255)
                                        ccGGGTTTTCCAGTCCTTTCAGCGCATAAGGGACCGCCATTAACGCATTGGTAAAAATCACAATGCCGCCAGCAGATGGCGGCAGGCCATTGGTGTTG‑TTGAGCAGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACATGCCGCTCATCTCCAGCG‑CCTGACCCGCCAGCATTTTCTGCCGCGCCCGAAGTTCGCGACTGCTCCATAGCA‑GCATCATGGT‑CAGCACTACGcac                                                                                                                                                                                                          <  1:873943/250‑1 (MQ=255)
                                          gggTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGCGGCAGGCCAATGGTGTTG‑TTGAGCAGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACATGCCGCTCATCTCCAGCG‑CCTGACCCGCCAGCATTTTCTGCCGCGCCCGAAGTTCGCGACTGCTCCATAGCA‑GCATCCTGGG‑CAGCACTACGcaca                                                                                                                                                                                                         >  2:846642/1‑250 (MQ=255)
                                            gTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGCGGCAGGCCAATGGTGTTG‑TTGAGCAGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACATGCCGCTCCTCTCCAGCC‑CCTGACACGCCAGCCTATTCTGCCGCGCCCGAATTTCGCGACTGCCCCATAGCC‑GCATCATGGG‑CAGCTCCACGCACAAt                                                                                                                                                                                                       >  2:474229/1‑250 (MQ=255)
                                             ttttCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGCGGCAGGCCAATGGTGTTG‑TTGAGCAGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACATGCCGCTCATCTCCAGCG‑CCTGACCCGCCAGCATTTTCTGCCGCGCCCGAAGTTCGCGACTGCTCCATAGCA‑GCATCATGGT‑CAGCACTACGCACAATac                                                                                                                                                                                                     >  1:273302/1‑251 (MQ=255)
                                              tttCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGCGGCAGGCCAAGGGTGTTG‑TGGAGCAGAAAAAAGAGGCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACATGCCGCTCATCCCCAGCG‑CCTGACCCGCCAGCATTTTCTGCCGCGCCCGAAGTTCGCGACTGCTCCATAGCA‑GCATCATGGT‑CAGCACTACGCACAATac                                                                                                                                                                                                     <  1:259924/250‑1 (MQ=255)
                                                tcaagatcTTTCAGCGCACAAGGGATCCCCATTAACGCTTTGGTGAAAACCACAAGCCCATCAGCAAATTGCGACAGGCCAATGGTTTTG‑TTGAGCAGTAAAAAGAGGCCGGTAGCCAGCACAACCCCCGGCGGGGCGAGGATCACCATGCCTCTCATTCCCAGCG‑CCTTACCCGCCAGCATTTTCTGCCGCGCCCGAAGTTCGCGACTGCTCCATAGCA‑GCATCATGGT‑CAGCACTACGCACAATacac                                                                                                                                                                                                   <  1:493406/243‑1 (MQ=255)
                                                   agtaCTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGCGGCAGGCCAATGGTGTTG‑TTGAGCAGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACATGCCGCTCATCTCCAGCG‑CCTGACCCGCCAGCCTTTTCTGCCGCGCCCGCAGTTCGCGACTGCTCCCTAGCA‑GCATCATGGT‑CAGCACTACGCACAATACCCCTgc                                                                                                                                                                                               >  2:961730/4‑251 (MQ=255)
                                                     taCTTTCAGCGCCTAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTAAGAAGATTGCGGCAGGCCAATGGCGTTG‑TTGAGCACTCAAAAGACGCCGGTAGCCAGCACAATCACCGGCATGGCGAGGGTCACCATGCCGCTCATATCAACCA‑CCTGCCCCCCCAGCATTTTCTGCCGCGCCCGAAGTATGCGGCTGCTCCATAGCC‑GCATCAAGGT‑CCGCACTCCCCTCAATACCCCTgccc                                                                                                                                                                                             >  1:973219/2‑250 (MQ=255)
                                                     taCTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGCGGCAGGCCAATGGTGTTG‑TTGAGCAGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACATTCCGCTCATCTCCAGCG‑CCTGACCCGCCAGCATTTTCTGCCGCGCCCGAAGTTCCCGACTGCTCCATCGCC‑GCATCTTGGT‑CAGCACTACGCACAATACACCTgccc                                                                                                                                                                                             >  2:909386/2‑250 (MQ=255)
                                                           cAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGCGGCAGGCCAATGGTGTTG‑TTGAGCAGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACATGCCGCTCATCTCCAGCG‑CCTGACCCGCCAGCATTTTCTGCCGCGCCCGAAGTTCGCGACTGCTCCATAGCA‑GCATCATGGT‑CAGCACTACGCACAATACACCTGCCGCCAgcg                                                                                                                                                                                       >  1:791726/1‑251 (MQ=255)
                                                           cAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGCGGCAGGCCAATGGTGTTG‑TTGAGCAGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACATGCCGCCCATCTCCAGCG‑CCTGACCCGCCAGCATTTTCTGCCGCGCCCGAAGTTCGCGACTGCTCCATAGCC‑GCATCCTGGT‑CAGCACTACGCACAATACACCTGCCGCCAgcg                                                                                                                                                                                       >  2:389289/1‑251 (MQ=255)
                                                            aGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGCGGCAGGCCAATGGTGTTG‑TTGAGCAGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACATGCCGCTCATCTCCAGCG‑CCTGACCCGCCAGCATTTTCTGCCGCGCCCGAAGTTCGCGACTGCTCCATAGCA‑GCATCATGGT‑CAGCACTACGCACAATACACCTGCCGCCAgcgc                                                                                                                                                                                      >  1:263189/1‑251 (MQ=255)
                                                                    aGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGCGGCAGGCCAATGGTGTTG‑TTGAGCAGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACATGCCGCTCATCTCCAGCG‑CCTGACCCGCCAGCATTTTCTGCCGCGCCCGAAGTTCGCGACTGCTCCATAGCA‑GAATCATGGA‑CAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGc                                                                                                                                                                               >  1:575363/1‑250 (MQ=255)
                                                                       gATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGCGGCAGGCCAATGGTGTTG‑TTGAGCAGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACATGCCGCTCATCTCCAGCG‑CCTGACCCGCCAGCATTTTCTGCCGCGCCCGAAGTTCGCGACTGCTCCATAGCA‑GCATCATGGT‑CAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACg                                                                                                                                                                           >  1:242001/1‑251 (MQ=255)
                                                                        aTCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGCGGCAGGCCAATGGTGTTG‑TTGAGCAGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACATGCCGCTCATCTCCAGCG‑CCTGACCCGCCAGCATTTTCTGCCGCGCCCGAAGTTCGCGACTGCTCCATAGCA‑GCATCATGGT‑CAGCACTACGCACAATACACCTGCCCCCAGCACAAAACGCaa                                                                                                                                                                             >  2:951181/1‑248 (MQ=255)
                                                                        aTCGACATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGCGGCAGGCCAATGGTGTTG‑TTGAGCAGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACATGCCGCTCATCTCCAGCG‑CCTGACCCGCCAGCATTTTCTGCCGCGCCCGAAGTTCGCGACTGCTCCATAGCA‑GCATCATGGT‑CAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACg                                                                                                                                                                           >  1:533710/1‑250 (MQ=255)
                                                                           gCCATTAACGCATTGGTGAAAATCACAATGCCGCCAGCAGATTGCGGCTGGGCAATGGAGTTG‑TTTGGCAGTAAACAGAAGCCGGTAACCCGCACAATCCCCGGCATGGCGAGGGTCAACATGCCGCCCATCTCCCGCG‑CCTGACCCGCCAGCATATTCTGCCGCGCCCGAAGTTCGGGACTGCTCCATAGCA‑GAATCATGGT‑CAGCACTCCGCACCATACACCCGACGCCAGCGCagcaagcaacgcagt                                                                                                                                                                       >  2:344529/1‑237 (MQ=255)
                                                                             cATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGCGGCAGGCCCATGGTGTTG‑TTGAGCAGTAAAAAGAAGCCGGTAGCCCGCACAATCCCCGGCATGGCGAGGATCAACATGCCGCTCATCTCCAGCG‑CCTGACCCGCCAGCATTTTCTGCCGCGCCCGACGTTCGCGACTGCTCCATAGAA‑GCATCCTGGT‑CCGCACTACGCAACATACACCTGCCCCCCGCCCAAAACGCCACGCGGTcc                                                                                                                                                                     >  2:476331/1‑251 (MQ=255)
                                                                             aataaaCGCAGTGGTAAAAATCCCAATGCCGTCAGCATTTGGCGGCAGGCAAATGGTGTTG‑TGGAGCAGTAAAAAGAAGCCGGTAGCCAGCACACCCCCCGGCATGGCGAGGATCACCATGCCGCTCATCTCCAGCG‑CCTGACCCGCCAGCATTTTCTGCCGCGCCCGAAGTTCGCGACTGCTCCATAGCA‑GCATCATGGT‑CAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTcc                                                                                                                                                                     <  1:570301/247‑1 (MQ=255)
                                                                              aTTAACGCTTTGTTTAAAATATCAATGCCTGCAGCAGTTTCTGGGAGGCCAATGGTGTTG‑TTGAACAATAAAAAGAAGCCTTTAGCCAACATAAACCCCGCCTTGGCGAGGAACAACTTGCCGCTCTTCCCCAGCG‑CCTACCCCGCCAGCATTTTCTGCCGCGCCCGAAGTTCGCGACTGCTCCATAGCA‑GCATCATGGT‑CAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTcc                                                                                                                                                                     <  1:342550/250‑1 (MQ=255)
                                                                              aTTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGCGGCAGGCCAATGGTGTTG‑TTGAGCAGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACATGCCGCTCATCTCCAGCG‑CCTGACCCGCCAGCCTTTTCTGCAGCGCCCGAAGTTCGCGGCTGATCAATAGCA‑GCCTCCTGGT‑ACGCACTACGCACAATACACCTGCCGCCAGCGCCATACGCAACGacgtacc                                                                                                                                                                    >  2:354493/1‑245 (MQ=255)
                                                                               ttAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGCGGCAGGCCAATGGTGTTG‑TTGAGCAGTAAAAAGAAGACGGTAGCCAGCACCATCCCCGGCATGGCGAGGATCAACATGCCGCTCATCTCCAGCG‑CCTGACCCGCCAGCATTTTCTGCCGCGCCCGCAGTTCGCGACTGCTCCATAGCA‑GCATCCTGGT‑CAGCACTACGCACAATACACCTGCCCACACCGCCATCCGCAACGAgagcccc                                                                                                                                                                   >  2:65147/1‑245 (MQ=255)
                                                                                tAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGCGGCAGGCCAATGGTGTTG‑TTGAGCAGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACATGCCGCTCATCTCCAGCG‑CCTGACCCGCCAGCATTTTCTGCCGCGCCCGAAGTTCGCGACTGCTCCATAGCA‑GCCTCATGGT‑CAGCACTACCCACAATACACCTGCCGCCCACGCAATACGCAACGAGGTcccc                                                                                                                                                                   >  2:262220/1‑248 (MQ=255)
                                                                                   cGCATGGGTGAAAAACAAAATGCCGTCAGCAGATTGCGGCAGGCAAATGGTGTTG‑TGGAGCATTAAAAAGAAGCCGGTACCCAGCCCAATCCCCGGCATGGCGAGGATCAACATGCCGCTCATCCCCAGCC‑CCTGACCCGCCAGCATTTTCTGCCGCGCCCGAAGTTCGCGACTGCTCCATAGCA‑GCATCATGGT‑CAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACA‑gcg                                                                                                                                                              <  1:913753/251‑1 (MQ=255)
                                                                                        tGGTGAAAATCACAATGCCGTCAGCAGATTGCGGCAGGCCAATGGTGTTG‑TTGAGCAGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACATGCCGCTCATCTCCAGCG‑CCTGACCCGCCAGCATTTTCTGCCGCGCCCGAAGTTCGCGACTGCTCCATAGCA‑GCATCATGGT‑CAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACA‑GCGCCTGc                                                                                                                                                         >  1:821099/1‑251 (MQ=255)
                                                                                         ggTGAAAATCACAATGCCGTCAGCAGATTGCGGCAGGCCAATGGTGTTG‑TTGAGCAGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACATGCCGCTCATCTCCAGCG‑CCTGACCCGCCAGCATTTTCTGCCGCGCCCGAAGTTCGCGACTGCTCCATAGCA‑GCATCATGGT‑CCGCACTACGCCCAATACACCTGCCGCCAGCGCAATACACAACGAGGTCCACA‑GCGCCTGcc                                                                                                                                                        >  2:416954/1‑251 (MQ=255)
                                                                                          gTGAAAATCACAATGCCGTCAGCAGATTGCGGCAGGCCAATGGTGTTG‑TTGAGCAGTAACAAGAAGCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACATGCCGCTCATCTCCCGCG‑CCTGACCCGCCAGCATTTTCTGCCGCGCCCGACGTTCGCGACTGCTCCATAGCC‑GCATCATGGT‑CAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACA‑GCGCCTGcc                                                                                                                                                        >  1:964140/1‑250 (MQ=255)
                                                                                               aaTCACAATGCCGTCAGCAGATTGCGGCAGGCCAATGGTGTTG‑TTGAGCAGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACATGCCGCTCATCTCCAGCG‑CCTGACCCGCCAGCATTTTCTGCCGCGCCCGAAGTTCGCGACTGCTCCATAGCA‑GCATCATGGT‑CAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACA‑GCGCCTGCCACAGc                                                                                                                                                   >  1:838403/1‑250 (MQ=255)
                                                                                                  cacaATGCCGTCAGCAGATTGCGGCAGGCCAATGGTGTTG‑TTGAGCAGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACATGCCGCTCATCTCCAGCG‑CCTGACCCGCCAGCATTTTCTGCCGCGCCCGAAGTTCGCGACTGCTCCATAGCA‑GCATCATGGT‑CAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACA‑GCGCCTGCCACAGCACCg                                                                                                                                               >  1:396616/1‑251 (MQ=255)
                                                                                                   acaATGCCGTCAGCAGATTGCGGCAGGCCAATGGTGTTG‑TTGAGCAGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACATGCCGCTCATCTCCAGCG‑CCTGACCCGCCAGCATTTTCTGCCGCGCCCGAAGTTCGCGACTGCTCCATAGCA‑GCATCATGGT‑CAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACA‑GCGCCTGCCACAGCACCgg                                                                                                                                              >  1:363315/1‑251 (MQ=255)
                                                                                                    aaaTGCCGTCAGCAGATTGCGGCAGGCAATTGGTGTTG‑TGGAGCAGAAAAAAGAAGCCGGTAGCCAGAACAATCCCCGGCATGGCGAGGACCAACATGCCGCTCATCTCCAGCG‑CCTGACCCGCCAGCATTTTCTGCCGCGCCCGAAGTTCGCGACTGCTCCATAGCA‑GCATCATGGT‑CAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACA‑GCGCCTGCCACAGCACCGGt                                                                                                                                             <  1:769342/250‑1 (MQ=255)
                                                                                                        gCCGTCAGCAGATTGCGGCAGGCCAATGGTGTTG‑TTGAGCAGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACATGCCGCTCATCTCCAGCG‑CCTGACCCGCCAGCATTTTCTGCCGCGCCCGACGTTCGCGACTGCTCCATAGCA‑GCATCATGGT‑CAGCACTACGCACAATACACCTGCCGCCAGCGCCATACGCAACGAGGTCCACA‑GCGCCTCCCACAGCACCGGTtgtg                                                                                                                                         >  2:597229/1‑251 (MQ=255)
                                                                                                         ccgacagaagATTGCGGCAGGCCAATGGTTTTG‑TGGAGCAGAAAAAAAAACCCGGTACCCGCCACAACCCCCGGAATGGGGAGAACCAACATGCCGCTCATCTCCAGCC‑CCTGACCCGCCAGCATTTTCTGCCGCGCCCGAAGTTCGCGACTGCTCCATAGCA‑GCATCATGGT‑CAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACA‑GCGCCTGCCACAGCACCGGTTGTGc                                                                                                                                        <  1:779433/243‑1 (MQ=255)
                                                                                                           ggcagaagATTGCGGCAGGCC‑ATTGTGTTGGTTGAGCAGAAAAAAGAAGCCGGTAGCCACCACAACCCCCTGCATGGCGAGGACCACCTTGCCCCTCATCTCCAGCG‑ACTGACCCGCCAGCATGTTCTGCCGCGCCCGAAGTGCGCGACTGCTCCATAGCA‑GCATCATGGT‑CAGCACTACGCACAATACCCCTGCCGCCAGCGCAATACGCAACGAGGTCCACA‑GCGCCTGCCACAGCACCGGATGTGCCa                                                                                                                                      <  1:676252/245‑1 (MQ=255)
                                                                                                                 agATTGCGGCAGGCCCATGGTGTTG‑TTGAGCAGTCAAAAGAAGCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACATGCCGCTCATCTCCAGCG‑CCTGACCCGCCAGCATTTTCTGCCGCGCCCGAAGTTCGCGACTGCTCCATAGCA‑GCATCATGGT‑CAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACA‑GCGCCTGCCACAGCACCGGTTGTGCCAGCACtt                                                                                                                                >  1:279886/1‑251 (MQ=255)
                                                                                                                 agATTGCGGCAGGCCAATGGTGTTG‑TTGAGCAGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACATGCCGCTCATCTCCAGCG‑CCTGACCCGCCAGCATTTTCTGCCGCGCCCGAAGTTCGCGACTGCTCCATAGCA‑GCATCATGGT‑CAGCACTACGCCCAATACACCTGCCGCCAGCGCAAAACGCAACGAGGTCCCCA‑GCACCTGCCACAGCACCGGTTGTGCCAGCCCtt                                                                                                                                >  2:793782/1‑251 (MQ=255)
                                                                                                                       ctgCAGGCCAATGGTGTTG‑TTGAGCCGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACATGCCGCTCATCTCCAGCG‑CCTGACCCGCCAGCATTTTCTGCCGCGCCCGAAGTTCGCGACTGCTCCATAGCA‑CCATCATGGT‑CAGCACTACGCCCAATACACCTGCCGCCAGCGCCAACCGCAACGCAGTCCACA‑GCGCCTGCCACCGCACCGGTTGTGCCAGCACTTCCGGcc                                                                                                                          >  2:290168/3‑250 (MQ=255)
                                                                                                                       cggCAGGCCAATGGTGTTG‑TTGAGCAGTAAAAAGAACCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACATGCCGCTCATCTCCAGCG‑CCTGACCCGCCAGCCTTTTCTGCCGCGCCCGAAGTTCGCGACTGCTCCATAACA‑GCATCATGGT‑CAGCACTACGAACAATACACCTGCCGCCAGAGCAATACGCAACGAAG‑GCACAAGCCCCTGCCCCAACACCGGTTGGGCCAACACTTCAGGcc                                                                                                                          >  2:861800/2‑250 (MQ=255)
                                                                                                                          cAGGCCAATGGTGTTG‑TTGAGCAGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACATGCCGCTCATCTCCCGCG‑CCTGACCCGCCAGCATTTTCTGCCGCGCCCGAAGTTCGCGACTGCTCCATAGCA‑GCATCATGGT‑CAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACA‑GCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACt                                                                                                                       >  1:325941/1‑251 (MQ=255)
                                                                                                                           aGGCCAATGGTGTTG‑TTGAGCAGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACATGCCGCTCATCTCCAGCG‑CCTGACCCGCCAGCATTTTCTGCCGCGCCCGAAGTTCGCGACTGCTCCATAGCA‑GCATCATGGT‑CAGCACTACGCACAATACACCTGCCGCCAGCGCACTACGCAACGAGGTCCACA‑GCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTg                                                                                                                      >  2:832727/1‑251 (MQ=255)
                                                                                                                                            tgAGCAGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACATGCCGCTCATCTCCAGCG‑CCTGACCCGCCAGCATTTTCTGCCGCGCCCGAAGTTCGCGACTGCTCCATAGCA‑GCATCATGGT‑CAGCACTACACACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACA‑GCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTAGCCCATcg                                                                                                      >  1:94273/1‑251 (MQ=255)
                                                                                                                                                 aGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGATAAACATGCCGCTCATCTCCAGCG‑CCTGACCCGCCAGCATTTTCTGCCGCGCCCGAAGTTCGCGACTGCTCCATAGCA‑GCATCATGGT‑CAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACA‑GCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTAGCCCATCGACGAt                                                                                                 >  1:346457/1‑251 (MQ=255)
                                                                                                                                                  gTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACATGCCGCTCATCTCCAGCG‑CCTGAACCGCCAGCATTTTCTGCCGCGCCCGAAGTTCGCGACTGCTCCATAGCA‑GCATCATGGT‑CAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACA‑GCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTAGCCCATCGCCGATc                                                                                                >  1:779845/1‑251 (MQ=255)
                                                                                                                                                       aagaagCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACATGCCGCTCATCTCCAGCG‑CCTGACCCGCCAGCATTTTCTGCCGCGCCCGAAGTTCGCGACTGCTCCATAGCA‑GCATCATGGT‑CAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACA‑GCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTAGCCCATCGACGATCACCGc                                                                                           >  1:991085/1‑251 (MQ=255)
                                                                                                                                                              cGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACATGCCGCTCATCTCCAGCG‑CCTGACCCGCCAGCATTTTCTGCCGCGCCCGAAGTTCGCGACTGCTCCATAGCA‑GCATCATGGT‑CAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACA‑GCGCCTCCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTCGCCCATCGACGACCACCGCCAGCAAc                                                                                    >  2:931435/1‑251 (MQ=255)
                                                                                                                                                               ggTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACATGCCGCTCATCTCCAGCG‑CCTGACCCGCCAGCATTTTCTGCCGCGCCCGAAGTTCGCGACTGCTCCATAGCA‑GCATCATGGT‑CAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACA‑GCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTAGCCCATCGACGATCACCGCCAGCAACg                                                                                   >  1:934588/1‑251 (MQ=255)
                                                                                                                                                                    ccAGCACAATCCCCGGCATGGCGAGGATCAACATGCCGCTCATCTCCAGCG‑CCTGACCCGCCAGCATTTTCTGCCGCGCCCGAAGTTCGCGACTGCTCCATAGCA‑GCATCATGGT‑CAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACA‑GCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTAGCCCATCGACGATCACCGCCAGCAACGGTGgc                                                                              >  1:68931/1‑251 (MQ=255)
                                                                                                                                                                     cAGCACAATCCACGGCATGGCGAGGATCAACATGCCGATCATCCCCAGCA‑CCTGACCCGCCAGCATTTTCTGCCGCGACCGTAGTTCGCGACTGCTCCATAGCC‑GCATCATGGT‑CAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCACCGAGGTCCACA‑GCGCCTGCCACAGCCCCGGTTGTGCCAGCAATTCCGGCAACTGGCGATTTAGCCCATCGACGATCACAGCCAGCACCGGTGgcc                                                                             >  2:420721/1‑250 (MQ=255)
                                                                                                                                                                          caATCCCCGGCATGGCGAGGATCAACATGCCGCTCATCTCCAGCG‑CCTGACCCGCCAGCATTTTCTGCCGCGCCCGAAGTTCGCGACTGCTCCATAGCA‑GCATCATGGT‑CAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACA‑GCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTAGCCCATCGACGCTCACCGCCAGCAACGGTGGCAGCAAc                                                                        >  2:932783/1‑251 (MQ=255)
                                                                                                                                                                           aaTCCCCGGCATGGCGAGGATCAACATGCCGCTCATCTCCAGCG‑CCTGACCCGCCAGCATTTTCTGCCGCGCCCGAAGTTCGCGACTGCTCCATAGCA‑GCATCATGGT‑CAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACA‑GCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGCTTTAGCCCATCGACGATCACCGCCAGCAATGGTGGCAGCAAc                                                                        >  1:329918/1‑250 (MQ=255)
                                                                                                                                                                                  ggCATGGCGAGGATCAACATGCCGCTCATCTCCAGCG‑CCTGACCCGCCAGCATTTTCTGCCGCGCCCGAAGTTCGCGACTGCTCCATAGCA‑GCATCATGGT‑CAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACA‑GCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTAGCCCATCGACGATCACCGCCAGCAACGGTGGCAGCAAcagcagc                                                                  >  1:577083/1‑249 (MQ=255)
                                                                                                                                                                                    cATGGCGCGGATCAACATGCCGCTCATCTCCAGCG‑CCTGACCCGCCAGCATTTTCTGCCGCGCCCGAAGTTCGCGACTGCTCCATAGCA‑GCATCATGGT‑CAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACA‑GCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTAGCCCATCGACGATCACCGCCAGCAACGGCGGCAGCAAccgcaaccgcg                                                              >  2:407085/1‑245 (MQ=255)
                                                                                                                                                                                    cATGGCGAGGATCAACATGCCGCTCATCTCCAGCG‑CCTGACCCGCCAGCATTTTCTGCCGCGCCCGAAGTTCGCGACTGCTCCATAGCA‑GCATCATGGT‑CAGCACTACGCACAATACACCTGCCGCAAGCGCAATACGCAACGAGGTCCACA‑GCGCCTGCCACAGCACCGGTTGAGCCAGCACTTCCGGCAACTGGCGATTTAGCCCATCGACGATCACCGCCAGCAACGGTGGCAGCAAcagcagcaccg                                                              >  2:183659/1‑248 (MQ=255)
                                                                                                                                                                                       ggcgggtaTCAACATCCCGCCCATCCCCGGCC‑CCGGCCCCGCCAGCATTTTCGGCCGCGCCCGAAGTTCGCGACTCCCCCATAGCA‑GCTCCATGGT‑CACCACTACGCAAAATCCACCTGCCCCCAGCGCAATACGCAACGAGTCCCCCA‑GCGCCTGCCACAGCCCCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTAGCCCATCGACGATCACCGCCAGCAACGGTGGCAGCAACAGCAGCAGCGCCa                                                           <  1:500274/244‑1 (MQ=255)
                                                                                                                                                                                           aGGATCAACATGCCGCTCATCTCCAGCG‑CCTGACCCGCCAGCATTTTCTGCCGCGCCCGAAGTTCGCGACTGCTCCATAGCA‑GCATCATGGT‑CAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACA‑GCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTAGCCCATCGCCGATCACCGCCCGCAACGGTGGCAGCACCAGCAGCAGCGCCCGcac                                                       >  2:39916/1‑251 (MQ=255)
                                                                                                                                                                                                cAACATGCCGCTCATCTCCAGCG‑CCTGACCCGCCAGCATTTTCTGCCGCGCCCGAAGTTCGCGACTGCTCCATAGCA‑GCATCCTGGT‑CAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACA‑GCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTAGCCCATCGACGATCACCGCCAGCAACAGTGGCAGCAACAGCAGCAGCGCCAGCACAAtt                                                   >  2:97798/1‑250 (MQ=255)
                                                                                                                                                                                                 aaCATGCCGCTCATCTCCAGCG‑CCTGCCCCGCCAGCATTTTCTGCCGCGCCCGAAGTTCGCGACTGCTCCATAGCA‑GCATCATGGT‑CAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACA‑GCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTAGCCCATCGACGATCACCGCCAGCAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTa                                                  >  1:302197/1‑250 (MQ=255)
                                                                                                                                                                                                 aaCATGCCGCTCATCTCCAGCG‑CCTGACCCGCCAGCATTTTCTGACGCGCCCGCAGTTCGCGACTGCTCCATAGCC‑GCATCATGGT‑CAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCCAAGAGGTCCACA‑GCGCCAGCCACAGCACCGGTTGTGCCAGCACTTCCGACAACTGGCGATGTATCCCCTCGACGAACACCGCCCACAACGGTTGCAGCAACCGCAGCAGCGCCAGCCCAATTa                                                  >  2:803040/1‑250 (MQ=255)
                                                                                                                                                                                                  aCATGCCGCTCATCTCCAGCG‑CCTGACCCGCCAGCATTTTCTGCCGCGCCCGAAGTTCGCGACTGCTCCATAGCA‑GCATCATGGT‑CAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACA‑GCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTAGCCCATCGACGATCACCGCCAGCAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAac                                                >  1:835479/1‑251 (MQ=255)
                                                                                                                                                                                                     tGCCGCTCATCTCCAGCG‑CCTGACCCGCCAGCATTTTCTGCCGCGCCCGAAGTTCGCGACTGCTCCATAGCA‑GCATCATGGT‑CAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACA‑GCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTAGCCCATCGACGATCACCGCCAGCAACGGTGGAAGCAACAGCAGCAGCGCCAGCACAATTAAAAcc                                             >  1:259454/1‑251 (MQ=255)
                                                                                                                                                                                                      gCCGCTCATCTCCAGCG‑CCTGACCCGCCAGCATTTTCTGCCGCGCCCGAAGTTCGCGACTGCTCCATAGCA‑GCATCATGGT‑CAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACA‑GCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTAGCCCATCGACGATCACCGCCAGCAACGGTGGCAGCAACAGCAGCAGCCCCCGCACAATTAACACCg                                            >  2:483107/1‑251 (MQ=255)
                                                                                                                                                                                                      cccccctcctcccgcgccccagccccGCCAGCTTTTTCTGCCGCGCCCGAAGTTCGCGACTGCTCCAAAGAA‑GCTTCATGGT‑CAGCACTCCGCACAATACACCTGCCGCCAGCGAAATACGCACCGAGGTCCACA‑GCGCCTGCCACAGCCCCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTAGCCCATCGACGATCACCGCCAGCAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAaca                                               <  1:569243/226‑1 (MQ=255)
                                                                                                                                                                                                        cGCTCATCTCCAGCG‑CCTGACCCGCCAGCATTTTCTGCCGCGCCCGAAGTTCGCGACTGCTCCATAGCA‑GCATCATGGT‑CAGCACTACGCACAATCCACCTGCCGCCAGCGCAATACGCAACGAGGACCACA‑GCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTAGCCCATAGACGATCACCGCCAGCAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCgtg                                          >  1:650530/1‑251 (MQ=255)
                                                                                                                                                                                                         gCTCATCTCCAGCG‑CCTGACCCGCCAGCATTTTCTGCCGCGCCCGAAGTTCGCGACTGCTCCATAGCA‑GCATCATGGT‑CAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACA‑GCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTAGCCCATCGACGATCACCGCCAGCAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCgtgt                                         >  1:1303/1‑251 (MQ=255)
                                                                                                                                                                                                         gCTCATCTCCAGCG‑CCTGACCCGCCAGCATTTTCTGCCGCGCCCGAAGTTCGCGACTGCTCCATAGCA‑GCATCATGGT‑CAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACA‑GCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTAGCCCATCGACGATCACCGCCAGCAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCgtgt                                         >  1:346974/1‑251 (MQ=255)
                                                                                                                                                                                                         gCTCATCTCCAGCG‑CCTGACCCGCCAGCATTTTCTGCCGCGCCCGAAGTTCGCGACTGCTCCATAGCA‑GCATCATGGT‑CAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACA‑GCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTAGCCCATCGACGATCACCGCCAGCAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCgtgt                                         >  1:347000/1‑251 (MQ=255)
                                                                                                                                                                                                             aTCTCCAGCG‑CCTGACACGCCAGCATTTTCTGCCACGCCCAAAGTTCGCGACTGCTACATAGCA‑GCATCATGGT‑CAGCACTACGCACAATACACCTGCCGCCAGCGAAATACGCAACGAGGTCAACA‑GCGCCTGCCACAGCACCGGGTGGGCCCACACTTCCGGCAACTGGCGATGTAGCCAATCGCCGCACACCGCACGCACCGGTCGCAGCAAAAGCAGAAGCCCCAGCACAATTAACACCgctcgccc                                     >  2:883518/1‑244 (MQ=255)
                                                                                                                                                                                                                      cgccTGACCCGCCAGCATTTTCTGCCGCGCCCGAAGTTCGCGACTGCTCCATAGCA‑GCATCATGGT‑CAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACA‑GCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTATCCCATCGACGATCACCGCCAGCAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGc                             >  1:129598/3‑251 (MQ=255)
                                                                                                                                                                                                                        ccTGACCCGCCAGCATTTTCTGCCGCGCCCGAAGTTCGCGACTGCTCCATAGCA‑GCATCATGGT‑CAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACA‑GCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTAGCCCATCGACGATCACCGCCAGCAACGGTGGCAGCAACAGCAGCAGCGCCAGCCCAATTAACACCGTGCCGCCAATGCGGCt                            >  2:906293/1‑250 (MQ=255)
                                                                                                                                                                                                                             cccGCCAGCATTTTCTGCCGCGACCGAAGTTCGCGACTGCTCCATAGCA‑GCATCATGGT‑CAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGCGGTCCACA‑GCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGAAACTGGCGATTTAGCCCATCGACGATCACCGCCAGCAACGGGGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAAAGAGGCTATGc                        >  2:825788/1‑249 (MQ=255)
                                                                                                                                                                                                                               cGCCAGCATTTTCTGCCGCGCCCGAAATTCGCGACTGCTCCATAGCA‑GCATCATGGT‑CAGCACTACGCACAATACACCTGCCGCCCGCGCAATACGCAACGAGGTCCACA‑GCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTAGCCCATCGACGATCACCGCCAGCAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGAc                    >  1:665314/1‑251 (MQ=255)
                                                                                                                                                                                                                                gCCAGCATTTTCTGCCGCGCCCGAAGTTCGCGACTGCTCCATAGCA‑GCATCATGGT‑CAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACA‑GCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTAGCCCATCGACGATCACCGCCAGCAACGGTGGCAGCACCAGCAGCAGCGCCAGCACACTTAACACCGAGTCGCAAATGCGGCTATgaagccg                   >  2:76892/1‑245 (MQ=255)
                                                                                                                                                                                                                                 ccAGCATTTTCTGCCGCGCCCGAAGTTCGCGACTGCTCCATAGCA‑GCATCATGGT‑CAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACA‑GCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTAGCCCATCGACGATCACCGCCAGCACCGGTGGCAGCAACGGCAGCAGCGCCAGCCCAATTAACACCGTGTCGCACATGCGGCTATGCAGACg                   >  2:665676/1‑250 (MQ=255)
                                                                                                                                                                                                                                    gCATTTTCTGCCGCGCCCGAATTTCGCGACTGCCCCATAGCA‑GCA‑CAAGGTTCAGCACTACCCAAAATACCCCTGCCGCCAGCGCACTACGCAACGAGGTCCACA‑GCGCCTGCCACAGCACCGGTGGTGCCAGCACTTCCGGCAACTGGCGATTTAGCCCATCGACGATCACCGCCAGCAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCg               <  1:946701/251‑1 (MQ=255)
                                                                                                                                                                                                                                       ttttCTGCCGCGCCCGAAGTTCGCGACTGCTCCATAGCA‑GCATCATGGT‑CAACACTACCCACAATACACCTGCAGCCAGCGCAATACGCAACGTGGTCCACA‑GCGCCTGCCACAGCACCGGTTGTGCCAGCACTTACGGCAACTGGCGATTTAGCCCATCGACGATCACCGCCAGCAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTcc            >  1:912541/1‑251 (MQ=255)
                                                                                                                                                                                                                                          ccTGCCGCCCCCGAAGTGCGCGACTGCTCCATAGAA‑GCATCATGGT‑AAGCACTACGCACAATACCCCGGCCGCCAGAGCAATACGCACCGAGGCCCACA‑GCGCCTGCCACAGCCCCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTAGCCCATCGACGATCACCGCCAGCAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCgg          <  1:981107/249‑1 (MQ=255)
                                                                                                                                                                                                                                                  gcCCGAAGTTCGCGACTGCTCCATAGCA‑GCATCATGGT‑CAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACA‑GCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTAGCCCATCGACGATCACCGCCAGCAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGcc  >  1:49464/1‑250 (MQ=255)
                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                       
GACTGACATAACATGCTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGCACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGTGGCAGGCCGATAGTGTTG‑TTGAGCAGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACATGCCGCTCATCTCCAGCA‑CCTGACCCGCCAGCATTTTCTGCCGCGCCCGCAGTTCGCGACTGCTCCATAGCA‑GCATCATGGT‑CAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACA‑GCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTACCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCC  >  NC_000913/73200‑73689

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑