Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 64,896 A→G T295T (ACT→ACC)  polB ← DNA polymerase II

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091364,8960AG97.9% 273.2 / ‑2.0 95T295T (ACT→ACC) polBDNA polymerase II
Reads supporting (aligned to +/- strand):  ref base A (1/1);  new base G (44/47);  total (45/50)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.34e-01

GAGATGACCAAATGCCGCCACCGAACCGCCGTGTCGGTCCACCGGCAGGCCGTTCACCGTTGCCCGTTCGAGTAAAAATGGCATGATTTCAGTTTTGTGGAAGAT‑CTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTTGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGG‑TCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAGCTCCTGAGCGACAGTT‑TCCAGCGAGA‑ATGAAGAGAAATTCCAGAACGCGGA‑TTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCGCTATTATCGC‑GCCCAAGACGCAGCGGAAGACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAG  >  NC_000913/64653‑65139
                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                       
gagaTGACCAATCGTCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCTGTTCACCGTCGCTCTTTCGAGTAAAAATGGCATGATTTCAGTTTTATGTAAAAA‑CTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGG‑TCAATTGCGTCCATTCGATCCCACGGGTTAGCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTT‑TCCa                                                                                                                                                                                                                                                 <  1:618466/251‑1 (MQ=255)
        cAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCCGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAAT‑CTGCGACACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGGTTATCTTCGGCGCAACGGCGG‑ACAATTTCGTCCTTTTGATCCCCCGGGCTATCGATAGATTTTCCTTCCCCTAATAACTCCTGAGCGACGGTT‑TCCAGCGAGa‑a                                                                                                                                                                                                                                         >  1:898768/1‑250 (MQ=255)
                          ccccgTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATTATTTCAGTTTTATGGAAAAT‑CTGCGTCACCAGCTCGCAATCGTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGG‑TCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTT‑TCCAGCGAGA‑ATGAAGAGAAATTCCAGAAc                                                                                                                                                                                                                      <  1:513782/249‑1 (MQ=255)
                           cccgttgctgtctacaggCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTACGGAAAAT‑CTGCGGCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGG‑TCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTT‑GCCAGCGAGA‑ATGAAGAGAAATTCCAGAAcg                                                                                                                                                                                                                     <  2:358334/235‑1 (MQ=255)
                                ggcGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAAT‑CTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGG‑TCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTT‑TCCAGCGAGA‑ATGAAGAGAAATTCCAGAACGCGGA‑t                                                                                                                                                                                                               <  2:800480/249‑1 (MQ=255)
                                    gtctaCCGGCAGTCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAAT‑CTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTAGCTTCGGCGAAACGGCGG‑TCAATTTCATCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGGGCGACGGTT‑TCCAGCGAGA‑ATGAAGAGAAATTCCAGAACGCGGA‑TTTCa                                                                                                                                                                                                           <  1:793469/247‑1 (MQ=255)
                                        aCCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAAT‑CTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGACCAATTT‑GTCCATTCGATCCCACGGGTTTTTGAAAGATTTTTCCTCGCCCAATAAATCCTTAGCGACGGTG‑TCCCGCGAGA‑ATGAAGAGAAATTCCAGAAACCGGA‑TTTCAgcgc                                                                                                                                                                                                       >  2:746808/1‑251 (MQ=255)
                                        aCCGGCAGGCCGTTCACCGTCGCTCGGTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAAT‑CTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGG‑TCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTT‑TCCAGCGAGA‑ATGAAGAGAAATTCCAGAACGCGGA‑TTGCAg                                                                                                                                                                                                          <  2:937240/248‑1 (MQ=255)
                                             aaGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAAT‑CTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGG‑TCAATTTCGTCCATTCGGTTCCACGGGTTTTCGCAAGATTTTCCTTTGCCTAATAACTCCTGAGCGACGGAT‑TCCAGCGAGA‑ATGAAAAGAAATTCCAGAAAGCGGG‑TTTCAGCGcccca                                                                                                                                                                                                   >  2:228499/2‑247 (MQ=255)
                                              aGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAAT‑CTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGG‑TCCATTTCGTCCATTCGATCCCACGGGTTTTCGAGAGATTTTCCTTCGCCTAATAACTCCTGGGCGACGGTT‑TCCAGCGAGA‑ATGAAGAGAAATTCCAGAACGCGGT‑TTTCCGCGCCTCGAt                                                                                                                                                                                                 >  1:789825/1‑251 (MQ=255)
                                              aGGCCGTTCAACGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAAT‑CTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGAAGGTTTATCTTCGGCGAAACGGCGG‑TCAATTTTGTCCATTCAATCACACGGGGTTTAGATAGCTTATCCTATGCCTAATTACTCCCTCGCGACGGCT‑TCCAGCGAGG‑ATGTAGAGAAATTACAAAAAGGGGA‑TTTCAGCGCCtagcg                                                                                                                                                                                                 >  2:618221/1‑247 (MQ=255)
                                               ggCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAAT‑CTGCGTCTCCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGG‑TCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTT‑TCCAGCGAGA‑ATGAAGAGAAATTCCAGAACGCTGA‑TTTCAGCGCCTCGATa                                                                                                                                                                                                <  2:95767/251‑1 (MQ=255)
                                                gCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAAT‑CTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGG‑TCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTT‑TCCAGCGAGA‑ATGAAGAGAAATTCCAGAACGCGGA‑TTTCAGCGCCTCGATAc                                                                                                                                                                                               <  1:451963/251‑1 (MQ=255)
                                                 ccGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAAT‑CTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGG‑TCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTT‑TCCAGCGAGA‑ATGAAGAGAAATTCCAGAACGCGGA‑TTTCAGCGCCTCGATAcc                                                                                                                                                                                              <  1:13467/251‑1 (MQ=255)
                                                  cggttaCCGTCGCTCGTTCGAGTAAAAAAGGCTGGTTTTCAGTGTTATGGAAAAT‑CTGCGTCCCCAGCTCGCCATCTTTCAGGTTATAAGTCGACAGCGCAGGTGTATCTTCGGCGAACCGGCGG‑TCAATTTCGTCCATTCGATCCCACGGGTGATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTT‑TCCAGCGAGA‑ATGAAGAGAAATTCCAGAACGTGGA‑TTTCAGCGCCTCGATActg                                                                                                                                                                                             <  2:514980/246‑3 (MQ=255)
                                                  cGTTCACCGTCGCTCTTTCGAGTAAAAAGGGCATGATGGCAGGTTTATGGTAGAT‑CTGCGTCACCATCTCGCAATCTTTCAGGTTATAATGCGCCAGCACAGGTTTATCTTCGTCGAAACGGCGG‑TCAAGTTCGTCCATTCGATCCGTCGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTT‑TCCAGCGAGA‑ATGAAGAGAAATTCCAGATCGCGGA‑TTTCTGCGCCTCGAAACCg                                                                                                                                                                                             <  2:315376/251‑1 (MQ=255)
                                                  cGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAAT‑CTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGG‑TCAATTTCGTCCCTTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTT‑TCCAGCGAGA‑ATGACGGGAAATTCCAGAACGCGGC‑TTTCAGCGCCTCGATACCg                                                                                                                                                                                             >  2:339578/1‑251 (MQ=255)
                                                       aCCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAAT‑CTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGG‑TCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTT‑TCCAGCGAGA‑ATGAAGAGAAATTCCAGAACGCGGA‑TTTCAGCGCCTCGATACCGTCGAt                                                                                                                                                                                        <  1:62600/251‑1 (MQ=255)
                                                            cgctcGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAAT‑CTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGG‑TCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCTACGGTT‑TCCAGCGAGA‑ATGAAGAGAAATTCCAGAACGCGGA‑TTTCAGCGCCTCGATACCGTCGATAAtt                                                                                                                                                                                    >  1:183517/5‑250 (MQ=255)
                                                                    cGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAAT‑CTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGG‑TCAATTTCGTCCATTCGATCCCACGGGGTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTT‑TCCAGCGGGA‑ATGAAGAGAAATTCCAGAACGCGGA‑TTTCAGCGCCTCGATAACGTCGATAATTAACCGACCt                                                                                                                                                                           >  2:198777/1‑251 (MQ=255)
                                                                           aaaTGGCATGATTTCAGTTTTATGGAAAAT‑CTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGG‑TCAATTTCGTCCATTCGATCCCCCGGGTTATAGATAAATTTTCCTTCGCCTAATCACTCCTGAGCGACGGGT‑TCCAGCGGAA‑ATGAAAAGAAATTCCCGAACCCGGA‑TTTAAGCTCCCCGGTACCGTCGGTAATTAGCCGCCCttttacat                                                                                                                                                                    >  2:807709/1‑246 (MQ=255)
                                                                           aaaTGGCATGATTTCAGTTTTATGGAAAAT‑CTGAGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGG‑TCGATTTCGTCCATTCGGTCCCACGGGGTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGGGACGGTT‑TCAAGCGAGG‑ATGAAGAGAAATTCCAGAACGCGGA‑TTTCAGCGCCTCGATACCGTCGGTAATTAGCCGACCTTTAGccg                                                                                                                                                                    >  1:278791/1‑250 (MQ=255)
                                                                           aaaTGGCATGATATCAGTTTTATGGAAAAG‑CTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGG‑TCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTT‑TCCAGCGAGA‑ATGAAGAGAAATTCCAGAACGCGGA‑TTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCt                                                                                                                                                                    <  1:74351/251‑1 (MQ=255)
                                                                               agaaTGATTTCAGTTTTATGGAAAAT‑CTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGG‑TTACTTTCGTCCCTTCGCACCCCCGGGTTCTCGAAAGATATTCCTTCGCCTAATAACATCTGAGCGACGGTT‑TCCCACGAGA‑ATGAAAACAAAATACAGACCACGGG‑TTTCAGCTCCCCGATAACGTCAATAATTatacacactttagacacgg                                                                                                                                                                 >  2:820941/4‑232 (MQ=255)
                                                                                gCATGATTTCAGTTTTATGGAAAAT‑CTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGG‑TCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTT‑TCCAGCGAGA‑ATGAAGAGAAATTCCAGAACGCGGA‑TTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGc                                                                                                                                                                >  1:478131/1‑250 (MQ=255)
                                                                                  aTGATCTCAGTTTTATGGAA‑ATGCCGCGTCACTATCTCTCAATCTTTCAGTTTATAAGCTGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGG‑TCAATTTTGGCCATTCGATCCCTCGGGTTTACGATAGATTTGTCTTCGCCTAATAACTCCTGAGCGACGGTT‑TCCAGCGAGA‑ATGAAGAGAAATTCCAGAACGCGGA‑TTTCAGCGCCTCGATACCGGCGTTAATTAGCCGACCTTTAGCCTGGTCaat                                                                                                                                                             <  2:702146/251‑2 (MQ=255)
                                                                                       ttCAGTTTTATGGAAAAA‑CTGCGTCACCAACTCGCAAGATTGCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGT‑TCAATTTCGTCCATTCGATCCCACGGGTTATCGAGAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTT‑TCCAGCGAGA‑ATGAAGAGAAATTCCAGAACGCGGA‑TTTCAGCGCCACGATACCGTCGATAATTAGCCGACCTTTAGCTTGGGCAAAAAAg                                                                                                                                                         <  2:850288/250‑1 (MQ=255)
                                                                                          aGTTTTATGGAAAAT‑CTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGG‑TCAATTTCGTCCATTCGATCCCACGGGGTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGCCGGTT‑TCCAGCGAGA‑ATGAAGAGAAATTCCAGAACGCGGA‑TTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAAACGc                                                                                                                                                     >  1:508925/1‑251 (MQ=255)
                                                                                                 atggaaaagcTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGG‑TCAATTTCGTCCAGTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTT‑TCCAGCGAGA‑ATGAAGAGAAATTCCAGAACGCGGA‑TTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGttt                                                                                                                                                <  2:694179/241‑1 (MQ=255)
                                                                                                  ggAAAAT‑CTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGG‑TCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTT‑TCCAGCGAGA‑ATGAAGAGAAATTCCAGAACTCGGA‑TTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTa                                                                                                                                             <  1:989023/251‑1 (MQ=255)
                                                                                                  ggAAAAT‑CTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGG‑TCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTT‑TCCAGCGAGA‑ATGAAGAGAAATTCCAGAACGCGGA‑TTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTa                                                                                                                                             <  1:808272/251‑1 (MQ=255)
                                                                                                      aaT‑CTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGG‑TCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGCCGGTT‑TCCAGCGAGA‑ATGACGAGAAATTCCAGAACGCGGA‑TTTCAGCGCCTCGATACACTCGGAAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACTCCGTTTTTAAAGc                                                                                                                                         >  2:809482/2‑251 (MQ=255)
                                                                                                      aaT‑CTGCGTCACCAGCTCGCAATCTTTCAGGCTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGG‑TCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTT‑TCCAGCGAGA‑ATGAAGAGAAATTCCAGAACGCGGA‑TTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTAAAGc                                                                                                                                         >  1:489983/2‑251 (MQ=255)
                                                                                                          cTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGGTTATCTTCGGCGAAACGGCGG‑TCAATTTTGTCCATTCGATCCCACGGGTTTTCTATAGATTTTCCTTCGCCTAATAACTCCTGCGCGCCGGTT‑TCCAGCGAGA‑ATGAAGAGAAATTCCAGAACACGGA‑TTTCTGCGCCCCGATACCGGCGTGAATTAGACGGCCTGTAACCTGGGCAAAAAAGaagcgtgttttatagcccg                                                                                                                                      >  2:194925/1‑233 (MQ=255)
                                                                                                            gCGTCACCAGCTCGGAAGCTTTCATGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGG‑TCAATTTCGTCCAGTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTT‑TCCAGCGAGA‑ATGAAGAGAAATTCCAGAACGCGGA‑TTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTAAAGCCGt                                                                                                                                      <  2:857565/249‑1 (MQ=255)
                                                                                                            gCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGG‑TCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTT‑TCCAGCGAGA‑ATGAAGAGAAATTCCAGAACGCGGA‑TTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGc                                                                                                                                    <  1:968025/251‑1 (MQ=255)
                                                                                                            gCGTCACCAGCTCGCAATCGTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGG‑TCAATTTCGTCCATTCGATCCCACGGGTTAGCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTT‑TCCAGCGAGA‑ATGAAGAGAAATTCCAGAACGCGTA‑TTTCAGCTCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAA‑GACGCCGTTTTTAAAGCCg                                                                                                                                       <  2:704933/247‑1 (MQ=255)
                                                                                                            gCGTCACCAGCTCGCAATCGTTCAGGTGATAAGTCGTCAGCGCAGGTTTATCTTCGGCGAAACGGCGG‑TCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTT‑TCCAGCGAGA‑ATGAAGAGAAATTCCAGAACGCGGA‑TTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTAAAGCCGt                                                                                                                                      <  1:543863/249‑1 (MQ=255)
                                                                                                             cGTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGG‑TCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTT‑TCCAGCGAGA‑ATGAAGAGAAATTCCAGAACGCGGA‑TTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCt                                                                                                                                   <  1:728905/251‑1 (MQ=255)
                                                                                                              gTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCATCGCAGGTTTATCTTCGGCTAAACGGCGG‑TCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTT‑TCCAGCGAGA‑ATGAAGAGAAATTCCAGAACGCGGA‑TTTCAGCGCCTCGATACCGTCGATAAGTAGCTGACCTTTATCCTGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTc                                                                                                                                  <  2:399849/251‑1 (MQ=255)
                                                                                                              gTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGG‑TCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTT‑TCCAGCGAGA‑ATGAAGAGAAATTCCAGAACGCGGA‑TTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTc                                                                                                                                  <  1:110660/251‑1 (MQ=255)
                                                                                                              gTCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGG‑TCAATTTCGTCCATTCGATCCCACGGGGTATCGATAACTTTTCCTTCGCCTAATAACTCCTTAGCGACGGTT‑TCCAGCGGGA‑CTGGAGAGAAATTCCAAAACCCGGAATTTC‑GCGCCTCGGTACCGTCGATAATTAGCAGACCTCTAGCCTGGACAAAAAAAACACCGCTTTTAAAGCCgatccc                                                                                                                                  >  2:848132/1‑246 (MQ=255)
                                                                                                               tCACCAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGG‑TCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGGT‑TCCAGCGAGA‑ATGAAGAGAAAATCCAGAACGCGGA‑TTTCAGCGCCTCGATACCGTCGTTAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGATcg                                                                                                                                 >  1:994266/1‑251 (MQ=255)
                                                                                                                 aCCAGCTGGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGG‑ACCATTTCGTCCATTCGATCCCAAGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGAGACGGTT‑TCCAGCGCGA‑ATGAAGAGAAATTCCAGAACGCGGA‑TTTCAACGCCTCGCTACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTacaacactgctcgcg                                                                                                                               >  2:429466/1‑237 (MQ=255)
                                                                                                                   cAGCTCGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGG‑TCAATTTCGTCCCTTCGATCCCACGGGTTATCGATAGCTTTTCCTTCGCCTAATAAATCCTGTGCGACGGTT‑TCCAGCGAGG‑ATGAAGAGAAATTCCCAAACGCGGA‑TTTTAGCACCTCGATACCGTCGAGAATTAACCGACCTTTAGCCCGGGCAAAAAAGACGACGTTTTTAAAACCggcacgccccc                                                                                                                             >  2:128705/1‑241 (MQ=255)
                                                                                                                        cGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGG‑TCCATTTCGGCCATTCGATCCCACGGGTTATCGGTAGATTTTCCTTCGCCTAATAACTCCTGGGCGACGGTT‑TCCTGCG‑GACATGCAGAAGAATTCCCGAACGCGGA‑TTTCACCGCCACGCTACCGTCGTAAATTAGCCGACCCTTACCCTGGGCAAAAAAGACCCCGTTTTTAAGACCGTGCTcgccccccccc                                                                                                                        >  2:556249/1‑243 (MQ=255)
                                                                                                                         gCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGCAACGGCGG‑TCAATTTCGTCCATTCGATCCCACGGGTTATAGATCAATGTTCCTTCGCCTACTAACTCCTGCGCGGCGGTT‑TCCAGCGAGA‑ATGAAGAGAAATTCCAGAACGCGGA‑TTTCAGCGCCTCGATAACGTCGATAATTAGCCGACCTATAACCTGGGCAAAAAAGACGCCGTTGTTAAAGCCGTGCTAGCGCCACTccc                                                                                                                       >  2:324011/1‑250 (MQ=255)
                                                                                                                          cAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGG‑TCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTT‑TCCAGCGAGA‑ATGAAGAGAAATTCCAGAACGCGGA‑TTTCAGCGCCTCGATACCGTCGATAATTAACCGACCTTTAGCCTGGGCAAAAAAGACGCCGTATTTAAAGCCGTGCTCGCGCCACTcccg                                                                                                                      >  2:660940/1‑249 (MQ=255)
                                                                                                                           aaTCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGG‑TCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTT‑TCCAGCGAGA‑ATGAAGAGAAATTCCAGAACGCGGA‑TTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGc                                                                                                                     >  1:635403/1‑251 (MQ=255)
                                                                                                                            atccttCAGGTTATAAGTGGCCAACGCCAGTTTATCTTCCGCGAAACGGCTG‑TCAATTTCGTCCAGTCGTGCCCCCGGGTTATCGATAGATTATCCGTCGCCTAATATCTCCTGAGCGACTGTT‑TCCAGCGAGA‑ATGAAGAGAAATTCCAGATCGCGTA‑TTCCAGCGCCTCGAGACCTTCGATAATTAGCCGACCTTTACCCTGGGCAGAAAAGACGCCGTTTTTAAAGCCGTGATCGCGCCACTCCAGCt                                                                                                                    <  2:43656/247‑1 (MQ=255)
                                                                                                                             tCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGG‑TCAATTTCGTCCATTCGATCCCAAGGGTTATCGGTAGATTTTCCTTCGCCTAATAACTCCTGGGCGGCGGGT‑TCCAGCGAGT‑ATGTAGAAAAATTTAAGAACGCGGG‑TTTCAGCGCCCCGATACCGTCGATAATTAGCCGACCTTTTGCCTGGGCAAAAAAGGAGCCGGTTTTAAAACCGTGCTTgcgcacctccacctc                                                                                                                   >  2:104840/1‑240 (MQ=255)
                                                                                                                                ttCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGG‑TCAATTTCGTCCATTCGATCCCACGGGGTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTT‑TCCAGCGAGA‑ATGAAGAGAAATTCCAGAACGCGGA‑TTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCCCGCGCCACTCCAGCTca                                                                                                                  <  2:419660/249‑2 (MQ=255)
                                                                                                                                    ggTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGG‑TCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTT‑TCCAGCGAGA‑ATGAAGAGAAATTCCAGAACGCGGA‑TTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTcactgtta                                                                                                            <  1:626451/251‑8 (MQ=255)
                                                                                                                                         aacggcgtcAGCGCAGGGTGATCTTCGGAGAACCTGCGG‑TCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGAGTTTCCTTCGCCTAATAACTCCTGGGCGACGGTT‑GCCAGCGAGA‑ATGAATAGAAATTCCATAACTCGGA‑TTTCAGCGCCTCGATACCGTCGATAATGAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTAAAGCCGGCCTCGCGCCACTCCAGCTCACTGTTATcgc‑g                                                                                                      <  2:470557/243‑1 (MQ=255)
                                                                                                                                                ccAGCGCAGGTTTATCTTCGGCGAAACGGCGG‑TCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTT‑TCCAGCGAGA‑ATGAAGAGAAATTCCAGAACGCGGA‑TTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGC‑Gccccagc                                                                                               >  1:611592/1‑247 (MQ=255)
                                                                                                                                                    cgcAGGTTTATCTTCGGCGAAACGGCGG‑TCAATTTCGTCCATTAGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTT‑TCCAGCGAGA‑ATGAAGAGAAATTCCAGAACGCGGA‑TTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGC‑GCCCCAGCCGCa                                                                                           <  1:887563/251‑1 (MQ=255)
                                                                                                                                                     gcAGGTTTATCTTCGGCGAAACGGCGG‑TCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTT‑TCCAGCGAGA‑ATGAAGAGAAATTCCAGAACGCGGA‑TTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGC‑Gccccagccgc                                                                                            >  1:462254/1‑242 (MQ=255)
                                                                                                                                                     gcAGGTTTATCTTCGGCGAAACGGCGG‑TCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTT‑TCCAGCGAGA‑ATGAAGAGAAATTCCAGAACGCGGA‑TTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGC‑GCCCCAGCCGCAg                                                                                          <  1:935608/251‑1 (MQ=255)
                                                                                                                                                       aGGTTTATCTTCGGCGAAACGGCGG‑TCAAATTCGCCCATTCGATACCACGGGTTATCGATAGATTTTCCTTCGCCTAATAAATCCTGCGCGACGGTT‑TCCCGCGGGG‑ATGAAAAGAAATTCCAGAACGCAGA‑TTTTAGCGCCTCGATACCGTCGATAACTAGCCGACCTTTAAACTGGGCAAAAAAGCCGCCGTTTTTAAAGCCGTGCTCGCGCCCCTCCAGCTAACTGTTATCGC‑Gccccagccgccgcg                                                                                        >  2:587659/1‑240 (MQ=255)
                                                                                                                                                         gTTTATCTTCGGCGAAACGGCGG‑TCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTT‑TCCAGCGAGA‑ATGAAGAGAAATTCCAGAACGCGGA‑TTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGC‑GCCCCAGCCGCAGCGGa                                                                                      <  1:351493/251‑1 (MQ=255)
                                                                                                                                                             aTCTTCGGCGAAACGGCGG‑TCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTT‑TCCAGCGAGA‑ATGAAGAGAAATTCCAGAACGCGGA‑TTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGC‑GCCCCAGCCGCAGCGGaata                                                                                   <  1:339578/250‑3 (MQ=255)
                                                                                                                                                             aTCTTCGGCGAAACGGCGG‑TCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTT‑TCCAGCGAGA‑ATGAAGAGAAATTCCAGAACGCGGA‑TTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACCCCAGCTCACTGTTATCGC‑GCCCCAGCCGCAGCgggata                                                                                   >  1:120250/1‑246 (MQ=255)
                                                                                                                                                                      gAAACTGCGG‑TCAATGTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTT‑TCCAGCGAGA‑ATGAAGAGAAATTCCAGAACGCGGA‑TTTCa                                                                                                                                                                                                           <  1:924499/122‑1 (MQ=255)
                                                                                                                                                                       aaaCGTCGG‑TCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTT‑TCCAGCGAGA‑ATGAAGAGAAATTCCAGAACGCGGA‑TTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGC‑GCCCCAGCCGCAGCGGAATACGGTAACGCTc                                                                        <  2:171737/251‑1 (MQ=255)
                                                                                                                                                                       aaaCGGCGG‑TCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTT‑TCCAGCGAGA‑ATGAAGAGAAATTCCAGAACGCGGA‑TTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGC‑GCCCCAGCCGCAGCGGAATACGGTAACGCTc                                                                        <  1:103569/251‑1 (MQ=255)
                                                                                                                                                                       aaaCGGCGG‑TCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTT‑TCCAGCGAGA‑ATGAAGAGAAATTCCAGAACGCGGA‑TTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGC‑GCCCCAGCCGCAGCGGAATACGGTAACGCTc                                                                        >  1:305255/1‑251 (MQ=255)
                                                                                                                                                                           ggcgg‑TCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTT‑TCCAGCGAGA‑ATGAAGAGAAATTCCAGAACGCGGA‑TTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGC‑GCCCCAGCCGCAGCGGAATACGGTAACGATCGGc                                                                     >  2:798476/1‑250 (MQ=255)
                                                                                                                                                                            gcgg‑TCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTT‑TCCAGCGAGA‑ATGAAGAGAAATTCCAGAACGCGGA‑TTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGC‑GCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCAt                                                                   <  1:930517/251‑1 (MQ=255)
                                                                                                                                                                              gg‑TCAATTTCGTCCATTCGATCCCACGGGTTATCTATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTT‑TCCAGCGAGA‑ATGAAGAGAAAGTCCAGAACGCGGA‑TTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGC‑GCCCCAGCCGCAGCGGAATACgg                                                                                <  2:548157/236‑1 (MQ=255)
                                                                                                                                                                              gg‑TCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTT‑TCCAGCGAGA‑ATGAAGAGAAATTCCAGAACGCGGA‑TTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGC‑GCCCCAGCCGCAGCGGAATACgg                                                                                >  1:548157/1‑236 (MQ=255)
                                                                                                                                                                              ag‑TCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCTCCTAATAACTCCTGAGCGACGGTT‑TCCAGCGAGA‑ATGAAGAGAAATTCCAGAACGCGGA‑TTTCAGCGCCTCGATACCGACGATAATTAGCCGACCTTTAGCCTGGGCAAAACAGACGCCGTTTTTAAAGCCGGGCTCGCGCCACACCAACTCACTGTTATCGC‑GCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGt                                                                 >  2:787095/2‑251 (MQ=255)
                                                                                                                                                                                  cAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTT‑TCCAGCGAGA‑ATGAAGAGAAATTCCAGAACGCGGA‑TTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGC‑GCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGttt                                                               <  1:660940/250‑1 (MQ=255)
                                                                                                                                                                                        cGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTT‑TCCAGCGAGA‑ATGAAGAGAAATTCCAGAACGCGGA‑TTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGC‑GCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTgcag                                                         <  1:389720/250‑1 (MQ=255)
                                                                                                                                                                                           ccATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTT‑TCCAGCGAGA‑ATGAAGAGAAATTCCAGAACGCGGA‑TTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGC‑GCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCAtt                                                     >  1:72330/1‑251 (MQ=255)
                                                                                                                                                                                           ccATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTT‑TCCAGCGAGA‑ATGAAGAGAAATTCCAGAACGCGGA‑TTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGC‑GCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCAtt                                                     >  2:297300/1‑251 (MQ=255)
                                                                                                                                                                                            catccgaccccACGGGTTACCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTT‑TCCAGCGAGA‑ATGAAGAGAAATTCCAGAACGCGGA‑TTTCAGCGCCTCGATACCGCCTATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGC‑GCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTc                                                    <  2:682494/243‑1 (MQ=255)
                                                                                                                                                                                             aTTCGATACCACGGGATATAGGTAGCTTTACCTTCGCCTAATAACTCCTGAGCGACGGTC‑TCCAGCGAGA‑ATGAAGAGCAATTCCAGAACGCGGA‑TTTCAGCGACTCGATACCATCGCTAATTAGCCGACCTTTCGCCTGGGCAAACAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGCCACCCATCA‑GCAGCGGCATACGGTACCGCTCGGAATGtttttccggcatacg                                                   >  2:410925/1‑241 (MQ=255)
                                                                                                                                                                                                cgaccccACGGGTTATCGTTAGATGTTCCTGCGCCTAAGAACTCCTGTGCGACGGTT‑TCCAGCGAGA‑ATGAAGAGAAATTCCAGACCGCGGA‑TTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGC‑GCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAg                                                <  2:954131/247‑1 (MQ=255)
                                                                                                                                                                                                cGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTT‑TCCAGCGAGA‑ATGAAGAGAAATTCCAGAACGCGGA‑TTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGC‑GCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCa                                                 <  1:840889/250‑1 (MQ=255)
                                                                                                                                                                                                 gATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTT‑TCCAGAGAGA‑ATGAAGAGAAATTCCAGAACGCGGA‑TTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGC‑GCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGc                                                  >  1:509447/1‑248 (MQ=255)
                                                                                                                                                                                                  aTCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGAC‑GTTCTCCAGCGAGA‑ATGAAGAGAAATTCCAGAACGCGGA‑TTTCAGCGCCTCGATCCCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGC‑GTCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGAt                                              <  1:406753/251‑1 (MQ=255)
                                                                                                                                                                                                    cccACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTT‑TCCAGCGAGA‑ATGAAGAGAAATTCCAGAACGCGGA‑TTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGC‑GCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGc                                                  >  1:327960/1‑245 (MQ=255)
                                                                                                                                                                                                      cACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTT‑TCCAGCGAGA‑ATGAAGAGAAATTCCAGAACGCGGA‑TTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGC‑ACCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTATTGCAGCATTCgacgaaatg                                           >  2:153279/1‑242 (MQ=255)
                                                                                                                                                                                                             tATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCGACGGTT‑TCCAGCGAGA‑ATGAAGAGAAATTCCAGAACGCGGA‑TTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGC‑GCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTGTGCAGCATTCGCAGATCGAACTGcac                                    <  1:788722/250‑1 (MQ=255)
                                                                                                                                                                                                             tATCGATAGATTTTCCTGCGCCGAATAACCCCTGAGCGACGGTT‑TCCAGCGAGA‑ATGAAGAGAAATTCCAGAACGCGGA‑TTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGC‑GCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGcaa                                    <  2:228135/250‑2 (MQ=255)
                                                                                                                                                                                                                cGATAGATTTTCATTCGCCTAATAACTCCTGAGCGACGGTT‑TCCAGCGAGA‑ATGAAGAGAAATTCCAGAACGCGGA‑TTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGC‑GCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACg                                >  1:741151/1‑251 (MQ=255)
                                                                                                                                                                                                                            cTTCGCCTAATAGCTCCTGAGCGACAGTT‑TCCAGCGAGA‑ATGAAGAGAAATTCTAGAACGCGGA‑TTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCGCTATTATCGC‑GCCCAAGACGCAGCGGAAGACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGt                               >  1:682542/1‑240 (MQ=255)
                                                                                                                                                                                                                            cTTCGCCTAATAGCCCGTGAGCGACAGTT‑TCCAGCGAGA‑ATGAAGAGAAATTCTAGAACGCGGA‑TTTCAGCGCCTCGATACCGTCGATAATTAGCCGCCCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCGCTATTATCGC‑GCCCAAGACGCAGCGGAAGACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGt                               <  2:682542/240‑1 (MQ=255)
                                                                                                                                                                                                                               cGCCTAAAAACTCCTGAGCTACGGTT‑TCCAGCGAGA‑ATGAAGAGAAATTCCAGAACTCGGA‑TTTCAGCGCCCCGATACCGTCGATAATTAGCCGACCTCTAGCCTGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGC‑GCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATc                 <  2:183517/251‑1 (MQ=255)
                                                                                                                                                                                                                                      taacTCCTGAGCGACGGTT‑TCCATCGAGA‑ATGAAGAGAAATTCCAGAACGCGGA‑TTTCAACGCCTCGATACCGTCGATACTTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGC‑GCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTCGCAGCATTCGCAGATCGCACTGCACCACTTTCCACCCgcgtatcacatc            >  2:611922/4‑238 (MQ=255)
                                                                                                                                                                                                                                        acTCCTGAGCGACGGTT‑TCCAGCGAGA‑ATGAAGAGAAATTCCAGAACGCGGA‑TTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGC‑GCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAgg         >  1:316078/2‑250 (MQ=255)
                                                                                                                                                                                                                                          tCCTGAGCGACGGTT‑TCCAGCGAGA‑ATGAAGAGAAATTCCAGAACGCGGA‑TTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGC‑GACCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCGTTCGCAGATCGAACTGCACCCCGTTCCAACCGAGGATAACATCAggccc      >  2:282778/1‑248 (MQ=255)
                                                                                                                                                                                                                                           ccTGAGCGACGGTT‑TCCAGCGAGA‑ATGAAGAGAAATTCCAGAACGCGGA‑TTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGC‑GCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCg     <  1:198777/251‑1 (MQ=255)
                                                                                                                                                                                                                                            cTGAGCGACGGTT‑TCCAGCGAGA‑ATGAAGAGAAATTCCAGAACGCGGA‑TTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAACGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGC‑GCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCCGGATCGt    >  2:229483/1‑251 (MQ=255)
                                                                                                                                                                                                                                              gAGCGACGGTT‑TACAGCGAGA‑ATGAAGAGAAATTCCAGAACGCGGA‑TTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGC‑GCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAg  <  1:676429/251‑1 (MQ=255)
                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                       
GAGATGACCAAATGCCGCCACCGAACCGCCGTGTCGGTCCACCGGCAGGCCGTTCACCGTTGCCCGTTCGAGTAAAAATGGCATGATTTCAGTTTTGTGGAAGAT‑CTGCGTCACCAGCTCGCAATCTTTCAGGTTATAAGTTGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGG‑TCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAGCTCCTGAGCGACAGTT‑TCCAGCGAGA‑ATGAAGAGAAATTCCAGAACGCGGA‑TTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCGCTATTATCGC‑GCCCAAGACGCAGCGGAAGACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAG  >  NC_000913/64653‑65139

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑