| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 73,214 | G→A | S436S (AGC→AGT) | thiP ← | thiamine ABC transporter membrane subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 73,214 | 0 | G | A | 99.2% | 406.1 / ‑6.4 | 134 | S436S (AGC→AGT) | thiP | thiamine ABC transporter membrane subunit |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (69/63); minor base C (0/1); total (69/65) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.81e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.55e-01 | |||||||||||
Only 100 of 134 total aligned reads displayed.
AACAGAGCAGCAGCAGAATTAACGCGGTGACCGCACCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACAACGCCACCACGCCAAAATCACCAATCG‑ACAGCACGCATGCAAAGGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCCACCACTTTTAAGCGTGACCAGCCTTCAATCCCCAGCGAC‑TGACAT‑AACATGCTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGCACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGTGGCAGGCCGATAGTGTTGTTGAGCAGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACATGCCGCTCATCTCCAGCACCTGACCCGCCAGCATTTTCTGCCGCGCCCGCAGTTCGCGACTGCT > NC_000913/72967‑73459 | aaCAGAGTAGCAGCAGAATTAACGCGGTGACCGCGCCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACAACGCCACCACGCCAAAATCACCAATCG‑ACAGCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACAC‑AACATACTg < 1:218672/251‑1 (MQ=255) aCAGAGTAGCAGCAGAATTAACGCGGTGACCGCGCCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAAGCATCGTTACCGAACAACGCCACCACGCCAAAATCACCAATCG‑ACAGCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACAC‑AACATACTGt < 2:574096/251‑1 (MQ=255) agagTAGCAGCAGAATTAACGCGGTGACCGCGCCGTCCTGGCTGCGATAGGAGCCAATTGGCTGGTAGAGATAAAACGGCAGTGTGCGGAAATCATCGTTACCGAACAACGCCACCACGCCAAAATCTCCAATCG‑ACAGCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGTACGTTTCAGGGCGCGCTGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACAC‑AACATACTGTAg < 1:77748/251‑1 (MQ=255) gcagcagAATTAACGCGGTGACCGCGCCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACAACGCCACCACGCCAAAATCACCAATCG‑ACAGCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACAC‑AACATACTGTAgcgggcg < 1:231233/251‑1 (MQ=255) aaCGCGGTGACCGCGCCGTCCTTGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACCACGCCACCACGCCAAAATCACCAATCG‑ACAGCACGCAGGCAAAAGCCAGCGCCTGCCCCAGTGGGCGTTTCAGGGCGCGCATCTCGACCACTTTTAAACGCGACCAGCCTTCAATGACCAGCGAC‑TGACAC‑AACATACTGTAGCGGGCGGGGATATcgcg > 2:709425/1‑251 (MQ=255) ggTGCCCGCGCCGTCCTGGCTGCGATAGGAGCCAATTTGCGGGTAGAGAGAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACAACGCCACCACGCCAAAATCACCAATCG‑ACAGCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACAC‑AACATACTGTAGCGGGCGGTGATATCGCGCATCg < 2:214994/251‑1 (MQ=255) gACCGCGCCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACAACGCCACCACGCCAAAATCACCAATCG‑ACAGCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACAC‑AACATACTGTAGCGGGCGGTGATATCGCGCATCGGGt < 1:715912/251‑1 (MQ=255) aCCGCGCCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACAACGCCACCACGCCAAAATCACCAATCG‑ACAGCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACAT‑AACATCCTGTAGCGGGCGGTGATATCGCGCATCGGGtt < 1:245499/251‑1 (MQ=255) ccGCGCCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACAACGCCACCACGCCAAAATCACCAATCG‑ACAGCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACAC‑AACATACTGTAGCGGGCGGTGATATCGCGCATCGGGttt > 1:754346/1‑251 (MQ=255) cgccGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACAACGCCACCACGCCAAAATCACCAATCG‑ACAGCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACAC‑AACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTcc > 1:716763/3‑251 (MQ=255) gccGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACAACGCCACCACGCCAAAATCACCAATCG‑ACAGCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGCCCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGTC‑TGACCC‑AACATAATGTAGCGGGCGGTGATATCGCCCATCGGGTTTTcc > 2:413351/2‑250 (MQ=255) gTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACAACGCCACCACGCCAAAATCACCAATCG‑ACAGCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACAC‑AACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAgt > 1:326087/1‑249 (MQ=255) ccTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACAACGCCACCACGCCAAAATCACCAATCG‑ACAGCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACAC‑AACATACTGTAGCGGGCGGTGATATCGCGCATCGGGGTTTCCAGTACt > 2:913753/1‑251 (MQ=255) ggCTGCGATAGGCGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACAACGCCACCACGCCAAAATCACCAATCG‑ACAGCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACAC‑AACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACtt < 2:104848/249‑1 (MQ=255) ggCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACAACGCCACCACGCCAAAATCACCAATCG‑ACAGCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACAC‑AACATACTGTAGCGTGCGGGGATAGCGCGCATCGGGTTTTCCAGTACttt < 1:776457/250‑1 (MQ=255) ggCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACAACGCCACCACGCCAAAATCACCAATCG‑ACAGCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TG‑CATCACCCTACTGTAGCGGGCGGTGATATCGCGCCTCGGGTTTTCCAGTACTTTc > 2:125786/1‑251 (MQ=255) ggCTGCGATAGGAGCCAAATTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGGTACCGAACAACGCCACCACGCCAAAATCACCAATCG‑ACAGCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACAC‑AACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACttac < 2:358735/251‑3 (MQ=255) ggCGATAGGAGCCAATTTTCTGGTAGAGATAAAACCGCAGGGTGCGGAAATCAACGTTACCGAACAACGCCACCACGCCAAAATCACCAATCG‑ACAGCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACAC‑AACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAgc < 2:32640/250‑1 (MQ=255) aTAGGAGCAAATTTGCAGGTAGAGATAAAACGGCAGGGTGCGGAAATCATAGTTACCGAACAACGCCACCACGACAAAATCAACCATCGCACA‑CACACAGGCAAAAGCCAGCGCCTGCGCCAAGGGACGTTTCAGGGCACGCAGCACGACCAATTTTAAGAGCGCACAGCATTCACTGCACCGCGGC‑TGCCAC‑AAACTACTGTAGAGGGCGGTGATATAGCGCATCGGGTTTTACAGGACtttaaacgca > 2:571738/1‑243 (MQ=255) aGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACAACGCCACCACGCCAAAATCACCAATCG‑ACAGCACGCAGGCAAAATCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCTCTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACAC‑AACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATaa < 1:662714/251‑1 (MQ=255) ggAGCCAATTTGCTGGTAGAGATAAAAGGGCAGGGGGCGGAAATCATCGTTACCGAACAACGCCACCACGCCAAAATCACCAATCG‑ACAGCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACAC‑AACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAg < 2:654608/251‑1 (MQ=255) aGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACAACGCCACCACGCCAAAATCACCAATCG‑ACAGCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑CGACAC‑AACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAggg > 1:396288/1‑251 (MQ=255) gCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACAACGCCACCACGCCAAAATCACCAATCG‑ACAGCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACAC‑AACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGa < 1:405773/251‑1 (MQ=255) gCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACAACGCCACCACGCCAAAATCACCAATCG‑ACAGCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACAC‑AACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCATTACTTTCAGCGCATAAGGGATCGCCAt < 1:485460/250‑1 (MQ=255) gCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACAACGCCACCACGCCAAAATCACCAATCG‑ACAGCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACAC‑AACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCAtt < 1:491606/251‑1 (MQ=255) cTGGTAGAGATAAAACGGCAGGGGGCGGAAATCATCGTTACCGAACGACGCCACCACGCCAAAATCACCAATCG‑CCAGCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGCCCACTTTTAAGAGCGACAAGCCTTCAATGCCCAGCGAC‑TGACAC‑AACATACTGTAGCGGGCGGAGATATCGCGCATCGGGTTTTCCAGCACTTTCAGCGCATAAGGGATCGccttt > 2:570301/1‑247 (MQ=255) gTAGAGATAAAACGTCAGGGTGCCGAAATCATCGTTACCGAACACCGCCACCACGCCAAAATCACCAATCG‑CCAGCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCACGACCACTATTAAGCGCGACCAGCCTTCAACGCCCAGCAAC‑TGACCC‑AACATACTTGAGGGGGCGGTGATATCGCGCCTCGGGTTTACCAGACCTTTCAGCGCATAAGGGGTCGACCTTAAcc > 2:877863/1‑250 (MQ=255) gTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACAACGCCACCACGCCAAAATCACCAATCG‑ACAGCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACAC‑AACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACg > 1:199761/1‑251 (MQ=255) agagATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACAACGCCACCACGCCAAAATCCCCAATCG‑ACAGCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACAC‑AACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCa < 1:71854/251‑1 (MQ=255) aTAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACAACGCCACCACGCCAAAATCACCAATCG‑ACAGCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACAC‑AACATACTGTAGCGGGCGGGGATATCGCGCATCGGGTTTTCCCGTACTTTCAGCGCCTAAGGGGACGCCATTACCGCCTTgg > 2:695171/1‑251 (MQ=255) tAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACAACGCCACCACGCCAAAATCACCAATCG‑ACAGCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACAC‑AACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTggg > 1:689602/1‑250 (MQ=255) aaaccGGCAGGGTTCGTAAATCATCGGTACCGAACAACGCTCCCACGCCAAAATCGCCAATCG‑ACAGCACGCAGTCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGAGCGCCGCTCGACCCCTTTTAAGCCCGACCAGCCTTCAATGCCCAGCGAC‑TGACAC‑AACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTTCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTg < 2:109604/247‑1 (MQ=255) aaaaCGGCAGGGTGCGGAATGCATCGTTACCGAACAACGCCACCCCGCCAAAAGCACCAATCG‑ACAGCACGCAGGCAAAAGCCAGCGACTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACAC‑AACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGt < 2:788689/250‑1 (MQ=255) cGGCAGGGTGCGGAAATCATCGTTACCGAACAACGCCACCACGCCAAAATCACCAATCG‑ACAGCACGCAGGCAACAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCACGCGAC‑TGACAC‑ACCATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCCTTAACGCCTTGGTGaaa > 2:898581/1‑250 (MQ=255) cGGCAGGGTGCGGAAATCATCGTTACCGAACAACGCCACCACGCCAAAATCACCAATCG‑ACAGCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACAC‑AACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCCTAAGGGATCGCCATTAACGCATTGGTGaaa > 1:326516/1‑250 (MQ=255) cAGGGTGCGGAAATCATCGTTACCGCACAACGCCACCACGCCAAAATCACCAATCG‑ACAGCACGCAGGCCACAGCCAGCGCCCGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCATTCAATGCCCAGCGAC‑CGACAC‑ACCATACTGTACCGGGCGGAGATATCGCGCATTGGGTTTTCCAGTACTTTCCGCGCCTAAGGCATCGCCCTTAACGCATTGGTgcagatc > 1:889125/1‑244 (MQ=255) cAGGGTGCGGAAATCATAGTTACCGAACAACGCCCCCACGCCAAAATCACCAATCG‑ACAGCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGATCGACCACTTTTAAGCGCGACCAGCCTTCACTGCCCAGCGCA‑TGACCC‑AACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATc > 2:128893/1‑250 (MQ=255) gccgAAATCATCGTTACCGAACAACGCCCCCACGCCAAAATCACCAATCG‑ACAGCACGCAGGCAAATGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACAC‑AACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATg < 1:814136/247‑1 (MQ=255) gCGGAAATCATCGTTACCGAACAACGCCACCACGCCAAAATCACCAATCG‑ACAGCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACAC‑AACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCAGTAACGCATTGGTGAAAATCACAATg < 1:278462/250‑1 (MQ=255) ggAAATCATCGTTACCGAACAACGCCACCACGCCAAAATCACCAATCG‑ACAGCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACAC‑AACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCg > 1:157538/1‑251 (MQ=255) aaaTCATAGTTACCGAACAACGCCACCACGCCAAAATCACCAATCG‑ACAGCACGCAGGCAAAAACCAGCGCCTGCGCCAGTGGACGATTCAGGGCGCGCAACTCGACCACTTTTAAGCGCGCCCAGCCTTCAATGCCCAGCGAC‑TGACAC‑AACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCCGTACTTTCAGCGCATAAGGGAGCGTCATTAACGCATTGATGAAAATCAAAATGCCGTc > 2:760044/1‑251 (MQ=255) atcatcGTTACCGAACAACGCCACAACGCCAAAATCACCAATCG‑ACAGCACGCAGGCAAAAGCCAGCGCCTGCGCAAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACAC‑AACATACTGTAGCGAGCGGGGATATAGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACACTGCCGTc > 1:816694/1‑249 (MQ=255) atcGTTACCGAACAACGCCACCACGCCAAAATCACCAATCG‑ACAGCACGCAGGCAAACGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACCC‑AACATACTGTAGCGGGCGGTGATATCGCGCATCGGGGTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGACAATCACATTGCCGTcagc > 2:695436/1‑249 (MQ=255) cGTTACCGAACAACGCCAACACGCCAAAATCACCAAACG‑ACAGCACGCAGGCAAAAGCCAGCGGCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCCCTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACAC‑ACCATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGAt < 1:688832/251‑1 (MQ=255) gTTACCGAACAACGCCACCACGCCAAAATCACCAATCG‑ACAGCACGCAGGCAAAAGCCAACGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACAACTTTTAAGCACGACCAGCCTTCAATGCCCAGCGAC‑TGACAC‑AACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGAtt > 1:73720/1‑251 (MQ=255) ttACCGAACAACGCCACCACGCCAACATCACCAATCG‑ACAGCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACAC‑AACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATtg > 1:693500/1‑251 (MQ=255) tACCGAACAACGCCACCACGCCAAAATCACCAATCG‑ACAGCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACAC‑AACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATtgc > 1:337075/1‑250 (MQ=255) tACCGAACAACGCCACCACGCCAAAATCACCAATCG‑ACAGCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACAC‑AACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGAAGATtgc > 1:451076/1‑250 (MQ=255) aCCGAACAACGCCACCACGCCAAAATCACCAATCG‑ACAGCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACAC‑AACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATtgcg < 1:776103/251‑3 (MQ=255) aCCGAACAACGCCACCACGCCAAAATCACCAATCG‑ACAGCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACAC‑AACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATtgcg > 1:922917/1‑249 (MQ=255) aCCGAACAACGCCACCACGCCAAAATCACCAATCG‑ACAGCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACAC‑AACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATtgc < 1:614265/250‑2 (MQ=255) cGAACAACGCCACCCCGCCAAAAACACCAAGCG‑ACAGCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCGCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACAC‑AACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCTTCAGCAGATTGCGGc < 2:822365/251‑1 (MQ=255) cGAACAACGCCACCACGCCAAAATCACCAATCG‑ACAGCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCACTGCCCAGCGAC‑TGACAC‑AACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGTTCGCCATTAACGCATTGGTGACAATCACAATGCCGTCCGCAGATTGCGGc > 1:684969/1‑251 (MQ=255) aacaacGCCACCACGCCAAAATCCCCAATCG‑ACAGCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACAC‑AACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGCGGCAg < 2:158000/251‑1 (MQ=255) aacaacGCCACCACGCCAAAATCACCAATCG‑ACAGCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACAC‑AACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGCGGCAg < 1:747418/251‑1 (MQ=255) acaacGCCACCACGCCAAAATCACCAATCG‑ACAGCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACAC‑AACATACTGTAGCGGGCGGTGATATCGCGCCTCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTcagcagctttcggcagg > 2:769342/1‑240 (MQ=255) aacGCCACCACGCCAAAATCACCAATCG‑ACAGCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACAC‑AACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGCGGCAGGcc > 1:941897/1‑251 (MQ=255) cGCCACCACGCCAAAATCACCAATCG‑ACAGCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACAC‑AACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGCGGCAGGccaa < 1:18069/251‑3 (MQ=255) gCCACCACGCCAAAATCACCAATCG‑ACAGCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACAC‑AACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGCGGCAGGccaa > 2:779433/1‑248 (MQ=255) caccacGCCAAAATCACCAATCG‑ACAGCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCCGCGAC‑TGACAC‑AACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACACATTGGTGCAACTCACAATGCCGTCAGCAGATTGCGGCAGGccactgg > 2:884806/1‑246 (MQ=255) accacGCCAAAATCACCAATCG‑ACAGCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACAC‑AACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGCGGCAGGccaatggt < 1:985207/251‑7 (MQ=255) ccccGCCAAAATCCCCAATCG‑ACAGCACGCAGGCAAAAGCCAGCGCCCGCGGCAGTGGACGGTTCAGGGCGCGCAGCTCGACCACTTTGAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACAC‑AACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGCGGCAGGccaatggtg < 2:756129/248‑8 (MQ=255) aaaTCACCAAACG‑ACAGCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCACGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACAC‑AACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGCGGCAGGCCAATGGTGTTGTTGAg < 2:328612/251‑1 (MQ=255) aaTCACCAATCG‑ACAGCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACAC‑AACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGCGGCAGGCCAATGGTGTTGTTGAGc > 1:260977/1‑251 (MQ=255) tCACCAATCG‑ACAGCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACAC‑AACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGCGGCAggacaatggtggttttgagcgg > 2:946701/1‑231 (MQ=255) caaacG‑ACAGCACGCAGGCAAAAGCCAGCGCCTGCGCCAGGGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACAC‑AACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGCGGCAGGCCAATGGTGTTGTTGAGCAGTaaa < 2:887357/247‑1 (MQ=255) cG‑ACAGCACGCAGGCAAAAGCCAGAGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACCC‑AACATACTGTAGCGGGCGGTGATATCGCGAATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGCAAATCACAATGCCGTCAGCAGATTGCGGCAGGcaaatggagtggtcgtgcagcaata > 2:92582/1‑224 (MQ=255) cAGCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGTACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCGTCAATGCCCAGCGAT‑TGACAC‑AACATACGGTAGCGGGGGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCTCAATGCCGTCAGCAGATTGCGGCAGGCCAATGGTGTTGTTGAGCAGTAAAAAGAAGc < 2:187090/251‑1 (MQ=255) aGCACGCAGGCAAAAGCAAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCCATGCCCAGCGAC‑TGACAC‑AACATACTGAAGCGGGCGGTGATATCGAGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGCTCGCCCTTAACGCATTGGTGAAACTCACACTGCCGTCAGCAGATTGCGGCAGGCCAATGGGGTTGTTGAGCAGGACAAAGAAGcc > 2:401714/1‑251 (MQ=255) aCGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGTCCACTTTTAAGCGCGACAAGCCTTCAATGCCCAGCGAC‑TGACAC‑AACATACGGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGCGGCAGGCCAAAGGTGTTGTTGAGCAGTAAAAAGACGCCGGt < 2:527090/251‑1 (MQ=255) aCGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACAC‑AACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGCTCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGCGGCAGGCCAATGGTGATGTTGAGCAGTAAAAAGAAGCCggg > 1:139710/1‑250 (MQ=255) cGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACAC‑AACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGCGGCAGGCCAATGGTGTTGTTGAGCAGTAAAAAGAAGCCGGt > 1:961744/1‑250 (MQ=255) gcaggCAAAAGCCTGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACAC‑AACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGCGGCAGGCCAATGGTGTTGTTGAGCAGTAAAAAGAAGCCGGTAg < 2:257442/247‑1 (MQ=255) gcaggCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACAC‑AACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGCGGCAGGCCAATGGTGTTGTTGAGCAGTAAAAAGAAGCCGGt > 1:916189/5‑249 (MQ=255) aaagCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACAC‑AACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGCGGCAGGCCAATGGTGTTGTTGAGCAGTAAAAAGAAGCCGGTAGCCAGcac > 1:479044/4‑251 (MQ=255) aagCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACAC‑AACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGCGGCAGGCCAATGGTGTTGTTGAGCAGTAAAAAGAAGCCGGTAGCCAGcac > 1:906810/3‑250 (MQ=255) gCCAGCGCCTGCGCCAGTGGACGGTTCAGGGCGTGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACAC‑AACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGCGGCAGGCCAATGGTGTTGTTGAGCAGTAAAAAGAAGCCGGTAGCCAGCACAAt < 2:834849/251‑1 (MQ=255) acaagggccggcgccgggggACGTTTCAGGGCGAGCAGCTCGACCACTTTGAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACAC‑AACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAAGGCCGTCAGCAGATTGCGGCAGGCCAATGGTGTTGTTGAGCAGTAAAAAGAAGCCGGTAGCCAGCACAAt < 2:412301/233‑1 (MQ=255) aGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACAC‑AACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAGTCACAATGCCGTCAGCACATTGCGGCAGGCCAATGGTGTTGTTGAGCAGTAAAAAGAAGCCGGGAACCACCACaaatcc > 2:321879/1‑247 (MQ=255) cgcCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTACAATGCCCAGCGAC‑TGACAC‑AACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGCGGCAGGCCAATGGTGTTGTTGAGCAGTAAAAATAAGCCGGTAGCCAGCACAATCCCCg < 1:705766/251‑1 (MQ=255) cgcCAGTGGACGTTTCAGGGCGCGCAGCGCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACAC‑AACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGCGGCAGGCCAATGGTGTTGTTGAGCAGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGCATgg < 2:245820/251‑1 (MQ=255) gcCAGTGGACGTGTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACAC‑AACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGCGGCAGGCCAATGGTGTTGTTGAGCAGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGCATGGc < 1:884806/251‑1 (MQ=255) ccAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACAC‑AACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGCGGCAGGCCAATGGTGTTGTTGAGCAGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGCATGGCg > 1:172429/1‑251 (MQ=255) cAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACAC‑AACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATAGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGCGGCAGGCCAATGGTGTTGTTGAGCAGTAAAAAGAAGCCGGGAGCAAGCACAATACCCGGCATgacg > 1:615284/1‑247 (MQ=255) cGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACAC‑AACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGCGGCAGGCCAATGGTGTTGTTGAGCAGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCa > 1:690955/1‑250 (MQ=255) ttCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACTC‑AACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGCGGCAGGCCAATGGTGTTGTTGAGCAGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACa < 1:125786/250‑1 (MQ=255) ttCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACAC‑AACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGCGGCAGGCCAATGGTGTTGTTGAGCAGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACa < 1:868964/250‑1 (MQ=255) ttCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCACAGCGAC‑TGACAC‑AACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGCGGCAGGCCAATGGTGTTGTTGAGCAGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACAt > 1:807414/1‑251 (MQ=255) tCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACAC‑AACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGCGGCAGGCCAATGGTGTTGTTGAGCAGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACAt < 1:295953/250‑1 (MQ=255) gggCGCGCCGCTCGACTACTGTTAAGGAGGACCAGCCGTCAATGCCCA‑TGACATGACAC‑AACATACTGTAGCGGGGGGGGATATCGCGCAGCGGGTTGTCCAGAGCGTTCAGCGCATAAGGGATCGCAATTAACGCAATGGGGAAAATCACAATGACGTCAGCAGGTTGAGGCAGGCCATTTGTGTTGTTGAGCAGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACATGcag < 2:737438/251‑3 (MQ=255) cggcgaaGCTCGACCACTTTTCAGCGGGACCAGCCTCCAATGCCCAGCGAC‑TGACAC‑AACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCCCAATGCCGTCAGCAGATTGCGGCAGGCCAATGGTGTTGTTGAGCAGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACATGCCGCt < 2:419630/245‑1 (MQ=255) cAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACAC‑AACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGCGGCAGGCCAATGGTGTTGTTGAGCAGTAAAAAGAAGCCGGTAGCCCGCACAATCCCCGGCATGGCGGGGAGCAACATGCCGCCCAtct > 2:42216/1‑251 (MQ=255) ctcgaccacTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACAC‑AACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACACTGCCGTCAGCAGATTGCGGCAGGCCAATGGTGTTGTTGAGCAGTAAAAAGAAGCCGGGAGCCCGCACAACCCCCGGCCTTGCGAGGATCAAAATGCCGCTcctcccc > 2:161854/5‑244 (MQ=255) cgaccacTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACAC‑AACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGCGGCAGGCCAATGGTGTTGTTGAGCAGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACATGCCGCTCATCTCCAGc < 1:379146/249‑1 (MQ=255) gaccacTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACAC‑AACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGCGGCAGGCCAATGGTGGTGTTGCGCAGTCAAAAGAAGCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACATGCCGCTCAtccacaacc > 2:525558/2‑244 (MQ=255) acTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACAC‑AACATACTGTAGCAGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGCTCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGCGGCAGGCCAATGGTGTTGTTGAGCAGTCAAAAGAAGCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGATAAACAAGACGCTAATCTCCaacgcctg > 2:761361/1‑244 (MQ=255) aatgccccgCGAC‑TGACAC‑AACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAACTCACAATGCCGTCAGCAGATTGCGGCAGGCCAATGGTGTTGTTGAGCAGTAAAAAGAAGCCGGGAGCCAGCCCAATCCCCGGCATGGCGAGGATCAACACTCCGCTCATCTCCAGCGCCTGACCCGCCAGCATTTTCTGCcgcc > 2:376599/9‑250 (MQ=255) aGCGAC‑TGACAC‑AACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGCGGCAGGCCAATGGTGTTGTTGAGCAGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACATGCCGCTCATCTCCAGCGCCTGACCCGCCAGCATTTTCTGCCGCGCCCga > 1:489982/1‑248 (MQ=255) gAC‑TGACAC‑AACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGCGGCAGGCCAATGGGGTTGTTGAGCAGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGCCTGGCGAGGCTCAACATGCCGCTCATCCCCAGCGCCTGACCCGCCAGCATTTTCTGCCGCGCCCGCAGTTc > 2:123502/1‑251 (MQ=255) gACAC‑AACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTCCAGCGCATAAGGGACCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGCGGCAGGCCAATGGTGTTGTTGAGCAGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACATGCCGCTCATCTCCAGCGCCGGACCCGCCAGCATTTTCTGCCGCGCCCGAAGTTcgcg < 1:597264/250‑1 (MQ=255) | AACAGAGCAGCAGCAGAATTAACGCGGTGACCGCACCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACAACGCCACCACGCCAAAATCACCAATCG‑ACAGCACGCATGCAAAGGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCCACCACTTTTAAGCGTGACCAGCCTTCAATCCCCAGCGAC‑TGACAT‑AACATGCTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGCACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGTGGCAGGCCGATAGTGTTGTTGAGCAGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACATGCCGCTCATCTCCAGCACCTGACCCGCCAGCATTTTCTGCCGCGCCCGCAGTTCGCGACTGCT > NC_000913/72967‑73459 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |