BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	73,193	C→G	G443G (GGG→GGC)	thiP ←	thiamine ABC transporter membrane subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	73,193	0	C	G	100.0%	490.9 / NA	144	G443G (GGG→GGC)	thiP	thiamine ABC transporter membrane subunit
Reads supporting (aligned to +/- strand): ref base C (0/0); new base G (76/68); total (76/68)

Only 100 of 146 total aligned reads displayed.

AATCACGGTAAACAGCAGAAAACAGAGCAGCAGCAGAATTAACGCGGTGACCGCACCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACAACGCCACCACGCCAAAATCACCAATCG‑ACAGCACGCATGCAAAGGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCCACCACTTTTAAGCGTGACCAGCCTTCAATCCCCAGCGAC‑TGACAT‑AACATGCTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGCACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGTGGCAGGCCGATAGTGTTGTTGAGCAGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACATGCCGCTCATCTCCAGCACCTGACCC  >  NC_000913/72947‑73421

aaTCACGGTAAACAGCATAAAACAGAGTAGCAGCCGAATTAACGCGGTGACCGCGCCGTCCTGGCTGCGATAGGAGCAAATTGTCTGGGAGAGATAAAACGGCAGGGTGCGGAAATCATCGGTACCGAACAACGCCACCACGCCAAAATCACCAAACG‑ACTGCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTGTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTGCAATGCCCa                                                                                                                                                                                                                                    <  2:97242/251‑1 (MQ=255)

 aTCACGGTAAACAGCAGAAAACAGAGTAGCAGCAGAATTAACGCGGTGACCGCGCCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACAACGCCACCACGCCAAAATCACCAATCG‑ACAGCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAACGCGACCAGCCTTCAATgccc                                                                                                                                                                                                                                     >  1:146537/1‑249 (MQ=255)

         aaaCAGCAGAAACCAGAGTAGCAGCAGCATTAACGCGGTGACCGCGCCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACAACGCCACCACGCCAAAATCACCAATCG‑ACAGCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCCCGACCAGACTTCACTGCCCAGCGAC‑Tgcc                                                                                                                                                                                                                          >  2:52521/1‑249 (MQ=255)

         aaaCAGCAGAAAACAGAGTAGCAGCAGAATTAACGCGGTGACCGCGCCGTCCTGGTTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAAGCATCGTTACCGAACAACGCCACCACGCCAAAATCGCCAATCG‑ACAGCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGTCGTTTCAGGGCGCGCAGCTCGACCTCTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGa                                                                                                                                                                                                                           <  2:892790/250‑1 (MQ=255)

         aaaCAGCAGAAAACAGAGTAGCAGCAGAATTAACGCGGTGACCGCGCCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACAACGCCACCACGCCAAAATCACCAATCG‑ACAGCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGAc                                                                                                                                                                                                                          <  1:183990/251‑1 (MQ=255)

          aaCAGCAGAAAACAGAGTAGCAGCAGAATTAACGCGGTGACCGCGCCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACAACGCCACCACGCCAAAATCACCAATCG‑ACAGCACGCAGGCAAAAGCCAGCGCATGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCgcatgacc                                                                                                                                                                                                                          >  2:624460/1‑244 (MQ=255)

           aCAGCAGAAAACAGAGTAGCAGCAGAATTAACGCGGTGACCGCGCCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACAACGCCACCACGCCAAAATCACCAATCG‑ACAGCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCCCGACCCCTTTTAAGCGCGACCAGCCTTCAATGCCCACCGCC‑TGACac                                                                                                                                                                                                                        >  2:259924/1‑250 (MQ=255)

             agcagAAAACAGAGTAGCAGCAGAATTAACGCGGTGACCGCGCCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACAACGCCACCACGCCAAAATCACCAATCG‑ACAGCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACaca                                                                                                                                                                                                                       >  1:466197/1‑248 (MQ=255)

              gcagAAAACAGAGTAGCAGCAGAATTAACGCGGTGACCGCGCCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTACGGAAATCATCGTTACCGAACAACGCCACCACGCCAAAATCACCAATCG‑ACAGCACGCAGGCAAAAGCCAGCGCCTGCGCCCGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACAC‑AAc                                                                                                                                                                                                                    >  1:391056/1‑251 (MQ=255)

               cagAAAACAGAGTAGCAGCAGAATTAACGCGGTGACCGCGCCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGAAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACAACGCCACCACGCCAAAATCACCAATCG‑ACAGCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACAC‑AAc                                                                                                                                                                                                                    >  1:443089/1‑250 (MQ=255)

                agAAAACAGAGTAGCAGCAGAATTAACGCGGTGACCGCGCCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACAACGCCACCACGCCAAAATCACCAATCG‑ACAGCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCCCTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑Tgccccaccct                                                                                                                                                                                                                   >  1:598563/1‑242 (MQ=255)

                  aaaaCTGAGTAGCAGCAGAATTAACGCGGTGACCGCGCCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACAACGCCACCACGCCAAAATCACCAATCG‑ACAGCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACAC‑AACAtac                                                                                                                                                                                                                >  1:477514/1‑249 (MQ=255)

                  aaaaCAGAGTAGCAGCAGAATTAACGCGGTGACCGCGCCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACAACGCCACCACGCCAAAATCACCAATCG‑ACAGCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACAC‑AACAtac                                                                                                                                                                                                                >  1:697318/1‑249 (MQ=255)

                    aaCAGAGTAGCAGCAGAATTAACGCGGTGACCGCGCCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACAACGCCACCACGCCAAAATCACCAATCG‑ACAGCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACAC‑AACATACTg                                                                                                                                                                                                              <  1:218672/251‑1 (MQ=255)

                       agagTAGCAGCAGAATTAACGCGGTGACCGCGCCGTCCTGGCTGCGATAGGAGCCAATTGGCTGGTAGAGATAAAACGGCAGTGTGCGGAAATCATCGTTACCGAACAACGCCACCACGCCAAAATCTCCAATCG‑ACAGCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGTACGTTTCAGGGCGCGCTGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACAC‑AACATACTGTAg                                                                                                                                                                                                           <  1:77748/251‑1 (MQ=255)

                             gcagcagAATTAACGCGGTGACCGCGCCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACAACGCCACCACGCCAAAATCACCAATCG‑ACAGCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACAC‑AACATACTGTAgcgggcg                                                                                                                                                                                                     <  1:231233/251‑1 (MQ=255)

                                        aaCGCGGTGACCGCGCCGTCCTTGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACCACGCCACCACGCCAAAATCACCAATCG‑ACAGCACGCAGGCAAAAGCCAGCGCCTGCCCCAGTGGGCGTTTCAGGGCGCGCATCTCGACCACTTTTAAACGCGACCAGCCTTCAATGACCAGCGAC‑TGACAC‑AACATACTGTAGCGGGCGGGGATATcgcg                                                                                                                                                                                          >  2:709425/1‑251 (MQ=255)

                                         ccgggctgtcctcgccGTCCTGGCGTCGATAGGAGCCAATTTGCTGATAGAGATAAATCGGCAGGGTGCGAAAATAATCGTTACCGAACAACGCCACCACGCCAAAATCACCAATCG‑ACAGCACGCAGGCAAAAGCCAGCGCCGTCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCTCTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACAC‑AACATACTGTAGCGGGCGGTGATATCgcgc                                                                                                                                                                                         <  2:530863/237‑1 (MQ=255)

                                                gACCGCGCCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACAACGCCACCACGCCAAAATCACCAATCG‑ACAGCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACAC‑AACATACTGTAGCGGGCGGTGATATCGCGCATCGGGt                                                                                                                                                                                  <  1:715912/251‑1 (MQ=255)

                                                     cgccGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACAACGCCACCACGCCAAAATCACCAATCG‑ACAGCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACAC‑AACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTcc                                                                                                                                                                             >  1:716763/3‑251 (MQ=255)

                                                      gccGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACAACGCCACCACGCCAAAATCACCAATCG‑ACAGCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCCCTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACAC‑AACATACTGTAGCGGGCAGTGATATCGCGCATCGGGGTTTccc                                                                                                                                                                            >  2:419891/2‑250 (MQ=255)

                                                         gTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACAACGCCACCACGCCAAAATCACCAATCG‑ACAGCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACAC‑AACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAgt                                                                                                                                                                          >  1:326087/1‑249 (MQ=255)

                                                           ccTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACAACGCCACCACGCCAAAATCACCAATCG‑ACAGCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACAC‑AACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACt                                                                                                                                                                       >  1:341271/1‑251 (MQ=255)

                                                           ccTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACAACGCCACCACGCCAAAATCACCAATCG‑ACAGCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACAC‑AACATACTGTAGCGGGCGGTGATATCGCGCATCGGGGTTTCCAGTACt                                                                                                                                                                       >  2:913753/1‑251 (MQ=255)

                                                            cgggCTGCGATAGGAGCCAATTTGCGGGTAGAGATAAAACGGCAGGGTGCGGAAAGCACCGTTACCGAACAACGCCACCACTCCAAAATCACCAATCG‑ACATCACCCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCTACCATCCTTCAATGCCCAGCGAC‑TGACAC‑AACATACTGTAGCGGGCGGTTATATCGCGCATCGGGTTTTCCAGTACtt                                                                                                                                                                      <  2:915478/249‑1 (MQ=255)

                                                              ggCTGCGATAGGCGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACAACGCCACCACGCCAAAATCACCAATCG‑ACAGCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACAC‑AACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACtt                                                                                                                                                                      <  2:104848/249‑1 (MQ=255)

                                                              ggCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACAACGCCACCACGCCAAAATCACCAATCG‑ACAGCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TG‑CATCACCCTACTGTAGCGGGCGGTGATATCGCGCCTCGGGTTTTCCAGTACTTTc                                                                                                                                                                    >  2:125786/1‑251 (MQ=255)

                                                              ggCTGCGATAGGAGCCAAATTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGGTACCGAACAACGCCACCACGCCAAAATCACCAATCG‑ACAGCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACAC‑AACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACttac                                                                                                                                                                    <  2:358735/251‑3 (MQ=255)

                                                                 ggCGATAGGAGCCAATTTTCTGGTAGAGATAAAACCGCAGGGTGCGGAAATCAACGTTACCGAACAACGCCACCACGCCAAAATCACCAATCG‑ACAGCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACAC‑AACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAgc                                                                                                                                                                 <  2:32640/250‑1 (MQ=255)

                                                                     aTAGGAGCAAATTTGCAGGTAGAGATAAAACGGCAGGGTGCGGAAATCATAGTTACCGAACAACGCCACCACGACAAAATCAACCATCGCACA‑CACACAGGCAAAAGCCAGCGCCTGCGCCAAGGGACGTTTCAGGGCACGCAGCACGACCAATTTTAAGAGCGCACAGCATTCACTGCACCGCGGC‑TGCCAC‑AAACTACTGTAGAGGGCGGTGATATAGCGCATCGGGTTTTACAGGACtttaaacgca                                                                                                                                                              >  2:571738/1‑243 (MQ=255)

                                                                       aGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACAACGCCACCACGCCAAAATCACCAATCG‑ACAGCACGCAGGCAAAATCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCTCTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACAC‑AACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATaa                                                                                                                                                           <  1:662714/251‑1 (MQ=255)

                                                                       aGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACAACGCCACCACGCCAAAATCACCAATCG‑ACAGCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACAC‑AACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATa                                                                                                                                                            >  1:158000/1‑250 (MQ=255)

                                                                          ggCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACAACGCCACCACGCCAAAATCACCAATCG‑ACAGCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACAC‑AACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAggg                                                                                                                                                        <  2:827471/250‑1 (MQ=255)

                                                                          aGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACAACGCCACCACGCCAAAATCACCAATCG‑ACAGCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑CGACAC‑AACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAggg                                                                                                                                                        >  1:396288/1‑251 (MQ=255)

                                                                            ccAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACAACGCCACCACGCCAAAATCACCAATCG‑ACAGCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACAC‑AACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGAt                                                                                                                                                      <  1:720988/251‑1 (MQ=255)

                                                                               aTTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACAACGCCACCACGCCAAAATCACCAATCG‑ACAGCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACAC‑AACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGc                                                                                                                                                   >  1:486527/1‑251 (MQ=255)

                                                                                   gCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACAACGCCACCACGCCAAAATCACCAATCG‑ACAGCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACAC‑AACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCATTACTTTCAGCGCATAAGGGATCGCCAt                                                                                                                                                <  1:485460/250‑1 (MQ=255)

                                                                                   gCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACAACGCCACCACGCCAAAATCACCAATCG‑ACAGCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACAC‑AACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCAtt                                                                                                                                               <  1:491606/251‑1 (MQ=255)

                                                                                    cTGGTAGAGATAAAACGGCAGGGGGCGGAAATCATCGTTACCGAACGACGCCACCACGCCAAAATCACCAATCG‑CCAGCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGCCCACTTTTAAGAGCGACAAGCCTTCAATGCCCAGCGAC‑TGACAC‑AACATACTGTAGCGGGCGGAGATATCGCGCATCGGGTTTTCCAGCACTTTCAGCGCATAAGGGATCGccttt                                                                                                                                               >  2:570301/1‑247 (MQ=255)

                                                                                       gTAGAGATAAAACGTCAGGGTGCCGAAATCATCGTTACCGAACACCGCCACCACGCCAAAATCACCAATCG‑CCAGCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCACGACCACTATTAAGCGCGACCAGCCTTCAACGCCCAGCAAC‑TGACCC‑AACATACTTGAGGGGGCGGTGATATCGCGCCTCGGGTTTACCAGACCTTTCAGCGCATAAGGGGTCGACCTTAAcc                                                                                                                                           >  2:877863/1‑250 (MQ=255)

                                                                                       gTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACAACGCCACCACGCCAAAATCACCAATCG‑ACAGCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACAC‑AACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACg                                                                                                                                           >  1:199761/1‑251 (MQ=255)

                                                                                             aTAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACAACGCCACCACGCCAAAATCACCAATCG‑ACAGCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACAC‑AACATACTGTAGCGGGCGGGGATATCGCGCATCGGGTTTTCCCGTACTTTCAGCGCCTAAGGGGACGCCATTACCGCCTTgg                                                                                                                                     >  2:695171/1‑251 (MQ=255)

                                                                                               aacaCGGCAGTGTGCGGAAAACACCGTTACCGAACAACCCCCCCACGCCAAAAACCCCAATCG‑GCAGCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACAC‑AACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTg                                                                                                                                   <  2:328672/248‑1 (MQ=255)

                                                                                               aaaaCGGCAGGGTGCGGAATGCATCGTTACCGAACAACGCCACCCCGCCAAAAGCACCAATCG‑ACAGCACGCAGGCAAAAGCCAGCGACTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACAC‑AACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGt                                                                                                                                    <  2:788689/250‑1 (MQ=255)

                                                                                                   cGGCAGGGTGCGGAAATCATCGTTACCGAACAACGCCACCACGCCAAAATCACCAATCG‑ACAGCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACAC‑AACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGaaaa                                                                                                                               <  1:25699/251‑1 (MQ=255)

                                                                                                     gcaggtagCGGAAATCATCGGTACCGAACACCGCCACCTCGCCAAAATCCCCAATCG‑ACAGCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTGCAGGGCGCGCAGCTCGACCTCTTTTAAGCGCGGCCAGCCTTCAAGGCCCAGCTAC‑TGACAC‑AACATACGTTCGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATGAGGAATCGACATGAACGCATTGGTGAAAATc                                                                                                                             <  2:409469/244‑1 (MQ=255)

                                                                                                      cAGGGTGCGGAAATCATCGTTACCGCACAACGCCACCACGCCAAAATCACCAATCG‑ACAGCACGCAGGCCACAGCCAGCGCCCGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCATTCAATGCCCAGCGAC‑CGACAC‑ACCATACTGTACCGGGCGGAGATATCGCGCATTGGGTTTTCCAGTACTTTCCGCGCCTAAGGCATCGCCCTTAACGCATTGGTgcagatc                                                                                                                             >  1:889125/1‑244 (MQ=255)

                                                                                                       aGGGTGCGGAAATCATCGTTACCGAACAACGCCACCACGCCAAAATCACCAATCG‑ACAGCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACAC‑AACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATcac                                                                                                                           >  2:597264/1‑251 (MQ=255)

                                                                                                           ggcgaaaaTCATCGTTACCGAACAACGCCACCACCCCAAAATCACCAATCG‑ACAGCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTGCAATGCCCAGCGAC‑TGACAC‑AACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCTATTAACGCATTGGTGAAAATCACAATg                                                                                                                       <  2:134547/246‑1 (MQ=255)

                                                                                                            gccgAAATCATCGTTACCGAACAACGCCCCCACGCCAAAATCACCAATCG‑ACAGCACGCAGGCAAATGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACAC‑AACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATg                                                                                                                       <  1:814136/247‑1 (MQ=255)

                                                                                                                  atcatcGTTACCGAACAACGCCACAACGCCAAAATCACCAATCG‑ACAGCACGCAGGCAAAAGCCAGCGCCTGCGCAAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACAC‑AACATACTGTAGCGAGCGGGGATATAGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACACTGCCGTc                                                                                                                  >  1:816694/1‑249 (MQ=255)

                                                                                                                     atcGTTACCGAACAACGCCACCACGCCAAAATCACCAATCG‑ACAGCACGCAGGCAAACGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACCC‑AACATACTGTAGCGGGCGGTGATATCGCGCATCGGGGTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGACAATCACATTGCCGTcagc                                                                                                               >  2:695436/1‑249 (MQ=255)

                                                                                                                       cGTTACCGAACAACGCCAACACGCCAAAATCACCAAACG‑ACAGCACGCAGGCAAAAGCCAGCGGCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCCCTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACAC‑ACCATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGAt                                                                                                           <  1:688832/251‑1 (MQ=255)

                                                                                                                        gTTACCGAACAACGCCACCACGCCAAAATCACCAATCG‑ACAGCACGCAGGCAAAAGCCAACGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACAACTTTTAAGCACGACCAGCCTTCAATGCCCAGCGAC‑TGACAC‑AACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGAtt                                                                                                          >  1:73720/1‑251 (MQ=255)

                                                                                                                         ttACCGAACAACGCCACCACGCCAACATCACCAATCG‑ACAGCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACAC‑AACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATtg                                                                                                         >  1:693500/1‑251 (MQ=255)

                                                                                                                          tACCGAACAACGCCACCACGCCAAAATCACCAATCG‑ACAGCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACAC‑AACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATtgc                                                                                                        >  1:337075/1‑250 (MQ=255)

                                                                                                                          tACCGAACAACGCCACCACGCCAAAATCACCAATCG‑ACAGCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACAC‑AACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATtg                                                                                                         <  1:613640/250‑1 (MQ=255)

                                                                                                                           aCCGAACAACGCCACCACGCCAAAATCACCAATCG‑ACAGCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACAC‑AACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATtgcg                                                                                                       >  1:922917/1‑249 (MQ=255)

                                                                                                                           aCCGAACAACGCCACCACGCCAAAATCACCAATCG‑ACAGCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACAC‑AACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATtgcg                                                                                                       <  1:776103/251‑3 (MQ=255)

                                                                                                                           aCCGAACAACGCCACCACGCCAAAATCACCAAACG‑ACAGCACGCAGGCAAAAGCCAGCGCCGGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACAC‑AACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATtgcg                                                                                                       <  2:122646/251‑3 (MQ=255)

                                                                                                                             cGAACAACGCCACCCCGCCAAAAACACCAAGCG‑ACAGCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCGCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACAC‑AACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCTTCAGCAGATTGCGGc                                                                                                     <  2:822365/251‑1 (MQ=255)

                                                                                                                             cGAACAACGCCACCACGCCAAAATCACCAATCG‑ACAGCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCACTGCCCAGCGAC‑TGACAC‑AACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGTTCGCCATTAACGCATTGGTGACAATCACAATGCCGTCCGCAGATTGCGGc                                                                                                     >  1:684969/1‑251 (MQ=255)

                                                                                                                             cGAACAACGCCACCACGCCAAAATCACCAATCG‑ACAGCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACAC‑AACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTAATTTCAGCGCATAAGGGATCGCCATAAACGCAATGGTGAAACTCACAATGCAGTCAGCAGATtgcgg                                                                                                      >  1:424962/1‑247 (MQ=255)

                                                                                                                               aacaacGCCACCACGCCAAAATCCCCAATCG‑ACAGCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACAC‑AACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGCGGCAg                                                                                                   <  2:158000/251‑1 (MQ=255)

                                                                                                                               aacaacGCCACCACGCCAAAATCACCAATCG‑ACAGCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACAC‑AACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGCGGCAg                                                                                                   <  1:747418/251‑1 (MQ=255)

                                                                                                                                acaacGCCACCACGCCAAAATCACCAATCG‑ACAGCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACAC‑AACATACTGTAGCGGGCGGTGATATCGCGCCTCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTcagcagctttcggcagg                                                                                                  >  2:769342/1‑240 (MQ=255)

                                                                                                                                  aacGCCACCACGCCAAAATCACCAATCG‑ACAGCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACAC‑AACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGCGGCAGGcc                                                                                                >  1:941897/1‑251 (MQ=255)

                                                                                                                                     gCCACCACGCCAAAATCACCAATCG‑ACAGCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACAC‑AACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGCGGCAGGccaa                                                                                              >  2:779433/1‑248 (MQ=255)

                                                                                                                                       caccacGCCAAAATCACCAATCG‑ACAGCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCCGCGAC‑TGACAC‑AACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACACATTGGTGCAACTCACAATGCCGTCAGCAGATTGCGGCAGGccactgg                                                                                           >  2:884806/1‑246 (MQ=255)

                                                                                                                                        accacGCCAAAATCACCAATCG‑ACAGCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACAC‑AACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGCGGCAGGccaatggt                                                                                          <  1:985207/251‑7 (MQ=255)

                                                                                                                                         ccccGCCAAAATCCCCAATCG‑ACAGCACGCAGGCAAAAGCCAGCGCCCGCGGCAGTGGACGGTTCAGGGCGCGCAGCTCGACCACTTTGAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACAC‑AACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGCGGCAGGccaatggtg                                                                                         <  2:756129/248‑8 (MQ=255)

                                                                                                                                                  aaTCACCAATCG‑ACAGCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACAC‑AACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGCGGCAGGCCAATGGTGTTGTTGAGc                                                                                >  1:260977/1‑251 (MQ=255)

                                                                                                                                                    tCACCAATCG‑ACAGCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACAC‑AACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGCGGCAggacaatggtggttttgagcgg                                                                              >  2:946701/1‑231 (MQ=255)

                                                                                                                                                      aCCAAACG‑ACAGCACGCAGGAAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACAC‑AACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGACGTCAGCAGATTGCGGCAGGCCAATGGTGTTGTTGAGCAGTa                                                                            <  2:198050/251‑1 (MQ=255)

                                                                                                                                                        caaacG‑ACAGCACGCAGGCAAAAGCCAGCGCCTGCGCCAGGGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACAC‑AACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGCGGCAGGCCAATGGTGTTGTTGAGCAGTaaa                                                                          <  2:887357/247‑1 (MQ=255)

                                                                                                                                                           tCG‑ACAGCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACAC‑ACCATACTGTAGCGGGCGGGGATATCGCGCATCGGGTTTTCCAGTCCTTTCAGCGCATAAGGGATCGCCATTAACGAATTGGTGAAAATCACAATGCCGTCAGCAGATTGCGGCAGGACCATGGGGTTGTTGAGACGTaaaaa                                                                        >  2:758544/1‑250 (MQ=255)

                                                                                                                                                                cAGCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGTACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCGTCAATGCCCAGCGAT‑TGACAC‑AACATACGGTAGCGGGGGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCTCAATGCCGTCAGCAGATTGCGGCAGGCCAATGGTGTTGTTGAGCAGTAAAAAGAAGc                                                                   <  2:187090/251‑1 (MQ=255)

                                                                                                                                                                cAGCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACAC‑AACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGCGGCAGGCCAATGGTGTTGTTGAGCAGTAAAAAGAAGc                                                                   >  1:689800/1‑251 (MQ=255)

                                                                                                                                                                    aCGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACAC‑AACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGCTCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGCGGCAGGCCAATGGTGATGTTGAGCAGTAAAAAGAAGCCggg                                                               >  1:139710/1‑250 (MQ=255)

                                                                                                                                                                     cGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACAC‑AACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGCGGCAGGCCAATGGTGTTGTTGAGCAGTAAAAAGAAGCCGGt                                                               >  1:961744/1‑250 (MQ=255)

                                                                                                                                                                      gcaggCAAAAGCCTGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACAC‑AACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGCGGCAGGCCAATGGTGTTGTTGAGCAGTAAAAAGAAGCCGGTAg                                                             <  2:257442/247‑1 (MQ=255)

                                                                                                                                                                      gcaggCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACAC‑AACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGCGGCAGGCCAATGGTGTTGTTGAGCAGTAAAAAGAAGCCGGt                                                               >  1:916189/5‑249 (MQ=255)

                                                                                                                                                                        aggCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACAC‑AACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGCGGCAGGCCAATGGTGTTGTTGAGCAGTAAAAAGAAGCCGGTAGcc                                                           >  1:324869/3‑251 (MQ=255)

                                                                                                                                                                              aagCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACAC‑AACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGCGGCAGGCCAATGGTGTTGTTGAGCAGTAAAAAGAAGCCGGTAGCCAGcac                                                      >  1:906810/3‑250 (MQ=255)

                                                                                                                                                                               agCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACAC‑AACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGCGGCAGGCCAATGGTGTTGTTGAGCAGTAAAAAGAAGCCGGTAGCCAGcaca                                                     >  1:76379/2‑250 (MQ=255)

                                                                                                                                                                                gCCAGCGCCTGCGCCAGTGGACGGTTCAGGGCGTGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACAC‑AACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGCGGCAGGCCAATGGTGTTGTTGAGCAGTAAAAAGAAGCCGGTAGCCAGCACAAt                                                   <  2:834849/251‑1 (MQ=255)

                                                                                                                                                                                acaagggccggcgccgggggACGTTTCAGGGCGAGCAGCTCGACCACTTTGAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACAC‑AACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAAGGCCGTCAGCAGATTGCGGCAGGCCAATGGTGTTGTTGAGCAGTAAAAAGAAGCCGGTAGCCAGCACAAt                                                   <  2:412301/233‑1 (MQ=255)

                                                                                                                                                                                   aGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACAC‑AACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAGTCACAATGCCGTCAGCACATTGCGGCAGGCCAATGGTGTTGTTGAGCAGTAAAAAGAAGCCGGGAACCACCACaaatcc                                                >  2:321879/1‑247 (MQ=255)

                                                                                                                                                                                           cgcCAGTGGACGTTTCAGGGCGCGCAGCGCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACAC‑AACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGCGGCAGGCCAATGGTGTTGTTGAGCAGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGCATgg                                        <  2:245820/251‑1 (MQ=255)

                                                                                                                                                                                            gcCAGTGGACGTGTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACAC‑AACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGCGGCAGGCCAATGGTGTTGTTGAGCAGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGCATGGc                                       <  1:884806/251‑1 (MQ=255)

                                                                                                                                                                                             ccAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACAC‑AACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGCGGCAGGCCAATGGTGTTGTTGAGCAGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGCATGGCg                                      >  1:172429/1‑251 (MQ=255)

                                                                                                                                                                                              cAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACAC‑AACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATAGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGCGGCAGGCCAATGGTGTTGTTGAGCAGTAAAAAGAAGCCGGGAGCAAGCACAATACCCGGCATgacg                                      >  1:615284/1‑247 (MQ=255)

                                                                                                                                                                                                     cGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACAC‑AACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGCGGCAGGCCAATGGTGTTGTTGAGCAGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCa                               >  1:690955/1‑250 (MQ=255)

                                                                                                                                                                                                        ttCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACTC‑AACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGCGGCAGGCCAATGGTGTTGTTGAGCAGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACa                            <  1:125786/250‑1 (MQ=255)

                                                                                                                                                                                                         tCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACAC‑AACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGCGGCAGGCCAATGGTGTTGTTGAGCAGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACAt                           <  1:295953/250‑1 (MQ=255)

                                                                                                                                                                                                           aGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACAC‑AACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGCGGCAGGCCAATGGTGTTGTTGAGCAGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACATGcc                        <  1:784634/251‑1 (MQ=255)

                                                                                                                                                                                                            gggCGCGCCGCTCGACTACTGTTAAGGAGGACCAGCCGTCAATGCCCA‑TGACATGACAC‑AACATACTGTAGCGGGGGGGGATATCGCGCAGCGGGTTGTCCAGAGCGTTCAGCGCATAAGGGATCGCAATTAACGCAATGGGGAAAATCACAATGACGTCAGCAGGTTGAGGCAGGCCATTTGTGTTGTTGAGCAGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACATGcag                       <  2:737438/251‑3 (MQ=255)

                                                                                                                                                                                                              cggcgaaGCTCGACCACTTTTCAGCGGGACCAGCCTCCAATGCCCAGCGAC‑TGACAC‑AACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCCCAATGCCGTCAGCAGATTGCGGCAGGCCAATGGTGTTGTTGAGCAGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACATGCCGCt                     <  2:419630/245‑1 (MQ=255)

                                                                                                                                                                                                                         gaccacTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACAC‑AACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGCGGCAGGCCAATGGTGGTGTTGCGCAGTCAAAAGAAGCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACATGCCGCTCAtccacaacc          >  2:525558/2‑244 (MQ=255)

                                                                                                                                                                                                                             acTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACAC‑AACATACTGTAGCAGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGCTCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGCGGCAGGCCAATGGTGTTGTTGAGCAGTCAAAAGAAGCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGATAAACAAGACGCTAATCTCCaacgcctg      >  2:761361/1‑244 (MQ=255)

AATCACGGTAAACAGCAGAAAACAGAGCAGCAGCAGAATTAACGCGGTGACCGCACCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACAACGCCACCACGCCAAAATCACCAATCG‑ACAGCACGCATGCAAAGGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCCACCACTTTTAAGCGTGACCAGCCTTCAATCCCCAGCGAC‑TGACAT‑AACATGCTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGCACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGTGGCAGGCCGATAGTGTTGTTGAGCAGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACATGCCGCTCATCTCCAGCACCTGACCC  >  NC_000913/72947‑73421

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`