BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	73,163	C→G	V453V (GTG→GTC)	thiP ←	thiamine ABC transporter membrane subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	73,163	0	C	G	100.0%	498.7 / NA	151	V453V (GTG→GTC)	thiP	thiamine ABC transporter membrane subunit
Reads supporting (aligned to +/- strand): ref base C (0/0); new base G (81/70); total (81/70)

Only 100 of 153 total aligned reads displayed.

AGTTTTAACATTTCGCCCCGGTAGTTTTTCAATCACGGTAAACAGCAGAAAACAGAGCAGCAGCAGAATTAACGCGGTGACCGCACCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACAACGCCACCACGCCAAAATCACCAATCG‑ACA‑GCACGCATGCAAAGGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCCACCACTTTTAAGCGTGACCAGCCTTCAATCCCCAGCGAC‑TGACATAACATGCTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGCACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGTGGCAGGCCGATAGTGTTGTTGAGCAGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACATGCCGCTCATCT  >  NC_000913/72917‑73407

agtTTTAACATCTCGCCCCGGTAGTTTTTCAATCACGGTAAACAGCAGAAAACAGAGTAGCAGCAGAATTAACGCGGTGACCGCGCCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACAACGCCACCACGCCAAAATCACCAATCG‑ACA‑GCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTcgacca                                                                                                                                                                                                                                                   <  1:181801/251‑1 (MQ=255)

 gtTTTAACATCTCGCCCCGGTAGTTTTTCAATCACGGTAAACAGCAGAAAACAGAGTAGCAGCAGAATTAACGCGGTGACCGCGCCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACAACGCCACCACGCCAAAATCACCAATCG‑ACA‑GCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCgaccac                                                                                                                                                                                                                                                  <  1:703281/251‑1 (MQ=255)

    ttAACATCTCGCCCCGGTAGTTTTTCAATCACGGTAAACAGCAGAAAACAGAGTAGCAGCAGAATTAACGCGGTGACCGCGCCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACAACGCCACCACGCCAAAATCACCAATCG‑ACA‑GCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTTGACGTTTCAGGGCGCGCAGCTCGACCACtt                                                                                                                                                                                                                                                <  1:724056/250‑1 (MQ=255)

    ttAACATCTCGCCCCGGTAGTTTTTCAATCACGGTAAACAGCAGAAAACAGAGTAGCAGCAGAATTAACGCGGTGACCGCGCCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACAACGCCACCACGCCAAAATCACCAATCG‑ACA‑GCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACttt                                                                                                                                                                                                                                               >  1:495484/1‑251 (MQ=255)

              gCCCCGGTAGTTTTTCCATCACGGTCAACAGCAGAAAACAGCGTAGCAGCAGAATTAACGCGGTGACCGCGCAGTCCTGGCTGCGATAGGAGCCAATTTGCTGGACGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGCACAACGCCACCACGCCCAAATCACCAATCG‑‑CACGCACGCAGGAAAAATCCAGCGCCTGCGCCAGTGGAAGTTTCAGAGCGAGCAGCACGAACACTTTTAAGCGAGAc                                                                                                                                                                                                                                     >  2:65040/1‑251 (MQ=255)

                cccGGTAGTTTTTCAATCACGGTAAACAGCAGAAAACAGAGTAGCAGCAGAATTAACGCGGAGACCGCGCCGTCCTCGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAAGGTGCGGAAGTCATCGTTACCTAACAACGCACCCACGCCAAAATCACCAATCG‑ACA‑GCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGGTGCAGAGCGCGCAGCTCGACAACTTTTAAACGCGAcc                                                                                                                                                                                                                                    >  1:936475/1‑250 (MQ=255)

                cccGGTAGTTTTTCAACCACGGTAAACAGCAGAAAACAGAGGAGCAGCAGAATTAACGCGGTGACCGCGCCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACTGCAGGGTGCGGAAATCATCGTTACCGAACAACGCCACCACTCCAAAATCACCAATCG‑ACA‑GCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCa                                                                                                                                                                                                                                   <  2:337507/251‑1 (MQ=255)

                 ccGGTAGTTTTTCAATCACGGTAAACAGCAGAAAACAGAGTAGCAGCAGAATTAACGCGGTGACCGCGCCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACAACGCCACCACGCCAAAATCACCAATCG‑ACA‑GCACGCAGGCAAAAGCCAGAGCCTGAGCCAGTGGACGTTTCAGGGCGCGCAGCTCGCCCACTTTTAAGCGCGAcc                                                                                                                                                                                                                                    >  2:278462/1‑249 (MQ=255)

                  cGGTAGTTTTTCAATCACGGTAAACAGCAGAAAACAGAGTAGCAGCAGAATTAACGCGGTGACCGCGCCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACAACGCCACCACGCCAAAATCACCAATCG‑ACA‑GCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGc                                                                                                                                                                                                                                 >  1:41143/1‑251 (MQ=255)

                       gTTTTTCAATCACGGTAAACAGCAGAAAACAGAGTAGCAGCAGAATTAACGCGGTGACCGCGCCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACAACGCCACCACGCCAAAATCACCAATCG‑ACA‑GCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTc                                                                                                                                                                                                                             >  1:412301/1‑250 (MQ=255)

                         ttttCAATCACGGTAAACAGCAGAAAACAGAGTAGCAGCAGAATTAACGCGGTGACCGCGCCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACAACGCCACCACGCCAAAATCACCAATCG‑ACA‑GCACGCAGGCAAAAGCCACCGCCTGCGCCAGGGGACGTTTCAGGGCGCGCAGCTCGACCCCTTTTAAGCGCGACCAGCCTTCAAt                                                                                                                                                                                                                          >  2:529987/1‑251 (MQ=255)

                                  aCGGTAAACAGCAGAAAACAGAGTAGCAGCAGAATTAACGCGGTGACCGCGCCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACAACGCCACCACGCCAAAATCACCAATCG‑ACA‑GCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCACTGCCCAGCgc                                                                                                                                                                                                                 >  2:873943/1‑250 (MQ=255)

                                       aaaCAGCAGAAACCAGAGTAGCAGCAGCATTAACGCGGTGACCGCGCCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACAACGCCACCACGCCAAAATCACCAATCG‑ACA‑GCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCCCGACCAGACTTCACTGCCCAGCGAC‑Tgcc                                                                                                                                                                                                           >  2:52521/1‑249 (MQ=255)

                                        aaCAGCAGAAAACAGAGTAGCAGCAGAATTAACGCGGTGACCGCGCCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACAACGCCACCACGCCAAAATCACCAATCG‑ACA‑GCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACa                                                                                                                                                                                                          <  1:489696/251‑1 (MQ=255)

                                        aaCAGCAGAAAACAGAGTAGCAGCAGAATTAACGCGGTGACCGCGCCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACAACGCCACCACGCCAAAATCACCAATCG‑ACA‑GCACGCAGGCAAAAGCCAGCGCATGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCgcatgacc                                                                                                                                                                                                           >  2:624460/1‑244 (MQ=255)

                                            gcagAAAACAGAGTAGCAGCAGAATTAACGCGGTGACCGCGCCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACAACGCCACCACGCCAAAATCACCAATCG‑ACA‑GCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACACAAc                                                                                                                                                                                                      <  2:824724/251‑1 (MQ=255)

                                             cagAAAACAGAGTAGCAGCAGAATTAACGCGGTGACCGCGCCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGAAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACAACGCCACCACGCCAAAATCACCAATCG‑ACA‑GCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACACAAc                                                                                                                                                                                                      >  1:443089/1‑250 (MQ=255)

                                              agAAAACAGAGTAGCAGCAGAATTAACGCGGTGACCGCGCCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACAACGCCACCACGCCAAAATCACCAATCG‑ACA‑GCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCCCTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑Tgccccaccct                                                                                                                                                                                                    >  1:598563/1‑242 (MQ=255)

                                                aaaaCTGAGTAGCAGCAGAATTAACGCGGTGACCGCGCCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACAACGCCACCACGCCAAAATCACCAATCG‑ACA‑GCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACACAACAtac                                                                                                                                                                                                  >  1:477514/1‑249 (MQ=255)

                                                   aCAGAGTAGCAGCAGAATTAACGCGGTGACCGCGCCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAAGCATCGTTACCGAACAACGCCACCACGCCAAAATCACCAATCG‑ACA‑GCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACACAACATACTGt                                                                                                                                                                                               <  2:574096/251‑1 (MQ=255)

                                                     agagTAGCAGCAGAATTAACGCGGTGACCGCGCCGTCCTGGCTGCGATAGGAGCCAATTGGCTGGTAGAGATAAAACGGCAGTGTGCGGAAATCATCGTTACCGAACAACGCCACCACGCCAAAATCTCCAATCG‑ACA‑GCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGTACGTTTCAGGGCGCGCTGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACACAACATACTGTAg                                                                                                                                                                                             <  1:77748/251‑1 (MQ=255)

                                                           gcagcagAATTAACGCGGTGACCGCGCCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACAACGCCACCACGCCAAAATCACCAATCG‑ACA‑GCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACACAACATACTGTAgcgggcg                                                                                                                                                                                       <  1:231233/251‑1 (MQ=255)

                                                                      aaCGCGGTGACCGCGCCGTCCTTGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACCACGCCACCACGCCAAAATCACCAATCG‑ACA‑GCACGCAGGCAAAAGCCAGCGCCTGCCCCAGTGGGCGTTTCAGGGCGCGCATCTCGACCACTTTTAAACGCGACCAGCCTTCAATGACCAGCGAC‑TGACACAACATACTGTAGCGGGCGGGGATATcgcg                                                                                                                                                                            >  2:709425/1‑251 (MQ=255)

                                                                       ccgggctgtcctcgccGTCCTGGCGTCGATAGGAGCCAATTTGCTGATAGAGATAAATCGGCAGGGTGCGAAAATAATCGTTACCGAACAACGCCACCACGCCAAAATCACCAATCG‑ACA‑GCACGCAGGCAAAAGCCAGCGCCGTCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCTCTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACACAACATACTGTAGCGGGCGGTGATATCgcgc                                                                                                                                                                           <  2:530863/237‑1 (MQ=255)

                                                                          cgGTGACCGCGCCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACAACGCCACCACGCCAAAATCACCAATCG‑ACA‑GCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACACAACATACTGTAGCGGGCGGTGATATCGCGCATc                                                                                                                                                                        <  1:212865/251‑1 (MQ=255)

                                                                               aCCGCGCCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACAACGCCACCACGCCAAAATCACCAATCG‑ACA‑GCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACATAACATCCTGTAGCGGGCGGTGATATCGCGCATCGGGtt                                                                                                                                                                   <  1:245499/251‑1 (MQ=255)

                                                                                ccGCGCCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACAACGCCACCACGCCAAAATCACCAATCG‑ACA‑GCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACACAACATACTGTAGCGGGCGGTGATATCGCGCATCGGGttt                                                                                                                                                                  >  1:754346/1‑251 (MQ=255)

                                                                                   cgccGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACAACGCCACCACGCCAAAATCACCAATCG‑ACA‑GCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACACAACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTcc                                                                                                                                                               >  1:716763/3‑251 (MQ=255)

                                                                                    gccGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACAACGCCACCACGCCAAAATCACCAATCG‑ACA‑GCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGCCCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGTC‑TGACCCAACATAATGTAGCGGGCGGTGATATCGCCCATCGGGTTTTcc                                                                                                                                                               >  2:413351/2‑250 (MQ=255)

                                                                                       gTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACAACGCCACCACGCCAAAATCACCAATCG‑ACA‑GCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACACAACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAgt                                                                                                                                                            >  1:326087/1‑249 (MQ=255)

                                                                                         ccTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACAACGCCACCACGCCAAAATCACCAATCG‑ACA‑GCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACACAACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACt                                                                                                                                                         >  1:341271/1‑251 (MQ=255)

                                                                                         ccTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACAACGCCACCACGCCAAAATCACCAATCG‑ACA‑GCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACACAACATACTGTAGCGGGCGGTGATATCGCGCATCGGGGTTTCCAGTACt                                                                                                                                                         >  2:913753/1‑251 (MQ=255)

                                                                                            ggCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACAACGCCACCACGCCAAAATCACCAATCG‑ACA‑GCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACACAACATACTGTAGCGTGCGGGGATAGCGCGCATCGGGTTTTCCAGTACttt                                                                                                                                                       <  1:776457/250‑1 (MQ=255)

                                                                                               ggCGATAGGAGCCAATTTTCTGGTAGAGATAAAACCGCAGGGTGCGGAAATCAACGTTACCGAACAACGCCACCACGCCAAAATCACCAATCG‑ACA‑GCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACACAACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAgc                                                                                                                                                   <  2:32640/250‑1 (MQ=255)

                                                                                                   aTAGGAGCAAATTTGCAGGTAGAGATAAAACGGCAGGGTGCGGAAATCATAGTTACCGAACAACGCCACCACGACAAAATCAACCATCGCACA‑‑CACACAGGCAAAAGCCAGCGCCTGCGCCAAGGGACGTTTCAGGGCACGCAGCACGACCAATTTTAAGAGCGCACAGCATTCACTGCACCGCGGC‑TGCCACAAACTACTGTAGAGGGCGGTGATATAGCGCATCGGGTTTTACAGGACtttaaacgca                                                                                                                                                >  2:571738/1‑243 (MQ=255)

                                                                                                     aGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACAACGCCACCACGCCAAAATCACCAATCG‑ACA‑GCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACACAACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATa                                                                                                                                              >  1:158000/1‑250 (MQ=255)

                                                                                                        ggCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACAACGCCACCACGCCAAAATCACCAATCG‑ACA‑GCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACACAACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAggg                                                                                                                                          <  2:827471/250‑1 (MQ=255)

                                                                                                        aGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACAACGCCACCACGCCAAAATCACCAATCG‑ACA‑GCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑CGACACAACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAggg                                                                                                                                          >  1:396288/1‑251 (MQ=255)

                                                                                                             aTTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACAACGCCACCACGCCAAAATCACCAATCG‑ACA‑GCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACACAACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGc                                                                                                                                     >  1:486527/1‑251 (MQ=255)

                                                                                                                     gTAGAGATAAAACGTCAGGGTGCCGAAATCATCGTTACCGAACACCGCCACCACGCCAAAATCACCAATCG‑CCA‑GCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCACGACCACTATTAAGCGCGACCAGCCTTCAACGCCCAGCAAC‑TGACCCAACATACTTGAGGGGGCGGTGATATCGCGCCTCGGGTTTACCAGACCTTTCAGCGCATAAGGGGTCGACCTTAAcc                                                                                                                             >  2:877863/1‑250 (MQ=255)

                                                                                                                     gTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACAACGCCACCACGCCAAAATCACCAATCG‑ACA‑GCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACACAACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACg                                                                                                                             >  1:199761/1‑251 (MQ=255)

                                                                                                                       agagATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACAACGCCACCACGCCAAAATCCCCAATCG‑ACA‑GCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACACAACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCa                                                                                                                           <  1:71854/251‑1 (MQ=255)

                                                                                                                           aTAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACAACGCCACCACGCCAAAATCACCAATCG‑ACA‑GCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACACAACATACTGTAGCGGGCGGGGATATCGCGCATCGGGTTTTCCCGTACTTTCAGCGCCTAAGGGGACGCCATTACCGCCTTgg                                                                                                                       >  2:695171/1‑251 (MQ=255)

                                                                                                                             aaaccGGCAGGGTTCGTAAATCATCGGTACCGAACAACGCTCCCACGCCAAAATCGCCAATCG‑ACA‑GCACGCAGTCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGAGCGCCGCTCGACCCCTTTTAAGCCCGACCAGCCTTCAATGCCCAGCGAC‑TGACACAACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTTCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTg                                                                                                                     <  2:109604/247‑1 (MQ=255)

                                                                                                                             aaaaCGGCAGGGTGCGGAATGCATCGTTACCGAACAACGCCACCCCGCCAAAAGCACCAATCG‑ACA‑GCACGCAGGCAAAAGCCAGCGACTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACACAACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGt                                                                                                                      <  2:788689/250‑1 (MQ=255)

                                                                                                                                 cGGCAGGGTGCGGAAATCATCGTTACCGAACAACGCCACCACGCCAAAATCACCAATCG‑ACA‑GCACGCAGGCAACAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCACGCGAC‑TGACACACCATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCCTTAACGCCTTGGTGaaa                                                                                                                  >  2:898581/1‑250 (MQ=255)

                                                                                                                                   gcaggtagCGGAAATCATCGGTACCGAACACCGCCACCTCGCCAAAATCCCCAATCG‑ACA‑GCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTGCAGGGCGCGCAGCTCGACCTCTTTTAAGCGCGGCCAGCCTTCAAGGCCCAGCTAC‑TGACACAACATACGTTCGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATGAGGAATCGACATGAACGCATTGGTGAAAATc                                                                                                               <  2:409469/244‑1 (MQ=255)

                                                                                                                                    cAGGGTGCGGAAATCATAGTTACCGAACAACGCCCCCACGCCAAAATCACCAATCG‑ACA‑GCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGATCGACCACTTTTAAGCGCGACCAGCCTTCACTGCCCAGCGCA‑TGACCCAACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATc                                                                                                               >  2:128893/1‑250 (MQ=255)

                                                                                                                                        gTGCGGAAATCATCGTTACCGAACAACGCCACCACGCCAAAATCACCAATCG‑ACA‑GCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACACAACATACTGTAGCGGGCGGGGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCCTTGGGGAAAACCACAAt                                                                                                          >  2:981107/1‑251 (MQ=255)

                                                                                                                                         ggcgaaaaTCATCGTTACCGAACAACGCCACCACCCCAAAATCACCAATCG‑ACA‑GCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTGCAATGCCCAGCGAC‑TGACACAACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCTATTAACGCATTGGTGAAAATCACAATg                                                                                                         <  2:134547/246‑1 (MQ=255)

                                                                                                                                          gccgAAATCATCGTTACCGAACAACGCCCCCACGCCAAAATCACCAATCG‑ACA‑GCACGCAGGCAAATGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACACAACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATg                                                                                                         <  1:814136/247‑1 (MQ=255)

                                                                                                                                          gCGGAAATCATCGTTACCGAACAACGCCACCACGCCAAAATCACCAATCG‑ACA‑GCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACACAACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCAGTAACGCATTGGTGAAAATCACAATg                                                                                                         <  1:278462/250‑1 (MQ=255)

                                                                                                                                            ggAAATCATCGTTACCGAACAACGCCACCACGCCAAAATCACCAATCG‑ACA‑GCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACACAACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCg                                                                                                      >  1:157538/1‑251 (MQ=255)

                                                                                                                                              aaaTCATAGTTACCGAACAACGCCACCACGCCAAAATCACCAATCG‑ACA‑GCACGCAGGCAAAAACCAGCGCCTGCGCCAGTGGACGATTCAGGGCGCGCAACTCGACCACTTTTAAGCGCGCCCAGCCTTCAATGCCCAGCGAC‑TGACACAACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCCGTACTTTCAGCGCATAAGGGAGCGTCATTAACGCATTGATGAAAATCAAAATGCCGTc                                                                                                    >  2:760044/1‑251 (MQ=255)

                                                                                                                                                acccacgtgaCCGAACAACGCCACCACGCCAAAATCACCAACCT‑ACA‑GCACGCAGGCAAAAGCCAGAGCCTGCGCCAGTGGACGTTTCAGGGCGCTCAGCTCGACCACGTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACACAACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCTTTAACGCATTGTTGAGAAGCACAATTCCGTcag                                                                                                  <  2:887249/242‑1 (MQ=255)

                                                                                                                                                   atcGTTACCGAACAACGCCACCACGCCAAAATCACCAATCG‑ACA‑GCACGCAGGCAAACGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACCCAACATACTGTAGCGGGCGGTGATATCGCGCATCGGGGTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGACAATCACATTGCCGTcagc                                                                                                 >  2:695436/1‑249 (MQ=255)

                                                                                                                                                     cGTTACCGAACAACGCCAACACGCCAAAATCACCAAACG‑ACA‑GCACGCAGGCAAAAGCCAGCGGCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCCCTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACACACCATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGAt                                                                                             <  1:688832/251‑1 (MQ=255)

                                                                                                                                                      gTTACCGAACAACGCCACCACGCCAAAATCACCAATCG‑ACA‑GCACGCAGGCAAAAGCCAACGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACAACTTTTAAGCACGACCAGCCTTCAATGCCCAGCGAC‑TGACACAACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGAtt                                                                                            >  1:73720/1‑251 (MQ=255)

                                                                                                                                                       ttACCGAACAACGCCACCACGCCAACATCACCAATCG‑ACA‑GCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACACAACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATtg                                                                                           >  1:693500/1‑251 (MQ=255)

                                                                                                                                                        tACCGAACAACGCCACCACGCCAAAATCACCAATCG‑ACA‑GCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACACAACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATtgc                                                                                          >  1:337075/1‑250 (MQ=255)

                                                                                                                                                        tACCGAACAACGCCACCACGCCAAAATCACCAATCG‑ACA‑GCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACACAACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGAAGATtgc                                                                                          >  1:451076/1‑250 (MQ=255)

                                                                                                                                                         aCCGAACAACGCCACCACGCCAAAATCACCAATCG‑ACA‑GCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACACAACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATtgc                                                                                          <  1:614265/250‑2 (MQ=255)

                                                                                                                                                         aCCGAACAACGCCACCACGCCAAAATCACCAAACG‑ACA‑GCACGCAGGCAAAAGCCAGCGCCGGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACACAACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATtgcg                                                                                         <  2:122646/251‑3 (MQ=255)

                                                                                                                                                           cGAACAACGCCACCACGCCAAAATCACCAATCG‑ACA‑GCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCACTGCCCAGCGAC‑TGACACAACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGTTCGCCATTAACGCATTGGTGACAATCACAATGCCGTCCGCAGATTGCGGc                                                                                       >  1:684969/1‑251 (MQ=255)

                                                                                                                                                           cGAACAACGCCACCACGCCAAAATCACCAATCG‑ACA‑GCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACACAACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGCGGc                                                                                       >  1:967682/1‑251 (MQ=255)

                                                                                                                                                              acaacGCCACCACGCCAAAATCACCAATCG‑ACA‑GCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACACAACATACTGTAGCGGGCGGTGATATCGCGCCTCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTcagcagctttcggcagg                                                                                    >  2:769342/1‑240 (MQ=255)

                                                                                                                                                              acaacGCCACCACGCCAAAATCACCAATCG‑ACA‑GCACGCAGGCAAAAGCCAGCGCCTGCACCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTACGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACACAACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTATTCCAGTACTTTCAGCGCATAAGGGCTCGCCATTAACGCATTGGTGAAAATCACACTGCCGTCAGCAGATTGCGGCAgg                                                                                    >  1:920787/1‑251 (MQ=255)

                                                                                                                                                                aacGCCACCACGCCAAAATCACCAATCG‑ACA‑GCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACACAACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGCGGCAGGcc                                                                                  >  1:941897/1‑251 (MQ=255)

                                                                                                                                                                  cGCCACCACGCCAAAATCACCAATCG‑ACA‑GCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACACAACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGCGGCAGGccaa                                                                                <  1:18069/251‑3 (MQ=255)

                                                                                                                                                                   gCCACCACGCCAAAATCACCAATCG‑ACA‑GCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACACAACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGCGGCAGGccaa                                                                                >  2:779433/1‑248 (MQ=255)

                                                                                                                                                                      accacGCCAAAATCACCAATCG‑ACA‑GCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACACAACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGCGGCAGGccaatggt                                                                            <  1:985207/251‑7 (MQ=255)

                                                                                                                                                                       ccccGCCAAAATCCCCAATCG‑ACA‑GCACGCAGGCAAAAGCCAGCGCCCGCGGCAGTGGACGGTTCAGGGCGCGCAGCTCGACCACTTTGAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACACAACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGCGGCAGGccaatggtg                                                                           <  2:756129/248‑8 (MQ=255)

                                                                                                                                                                               aaaTCACCAAACG‑ACA‑GCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCACGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACACAACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGCGGCAGGCCAATGGTGTTGTTGAg                                                                   <  2:328612/251‑1 (MQ=255)

                                                                                                                                                                                aaTCACCAATCG‑ACA‑GCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACACAACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGCGGCAGGCCAATGGTGTTGTTGAGc                                                                  >  1:260977/1‑251 (MQ=255)

                                                                                                                                                                                  tCACCAATCG‑ACA‑GCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACACAACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGCGGCAggacaatggtggttttgagcgg                                                                >  2:946701/1‑231 (MQ=255)

                                                                                                                                                                                    aCCAAACG‑ACA‑GCACGCAGGAAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACACAACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGACGTCAGCAGATTGCGGCAGGCCAATGGTGTTGTTGAGCAGTa                                                              <  2:198050/251‑1 (MQ=255)

                                                                                                                                                                                      caaacG‑ACA‑GCACGCAGGCAAAAGCCAGCGCCTGCGCCAGGGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACACAACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGCGGCAGGCCAATGGTGTTGTTGAGCAGTaaa                                                            <  2:887357/247‑1 (MQ=255)

                                                                                                                                                                                          cG‑ACA‑GCACGCAGGCAAAAGCCAGAGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACCCAACATACTGTAGCGGGCGGTGATATCGCGAATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGCAAATCACAATGCCGTCAGCAGATTGCGGCAGGcaaatggagtggtcgtgcagcaata                                                           >  2:92582/1‑224 (MQ=255)

                                                                                                                                                                                              cA‑GCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGTACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCGTCAATGCCCAGCGAT‑TGACACAACATACGGTAGCGGGGGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCTCAATGCCGTCAGCAGATTGCGGCAGGCCAATGGTGTTGTTGAGCAGTAAAAAGAAGc                                                     <  2:187090/251‑1 (MQ=255)

                                                                                                                                                                                              cA‑GCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACACAACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGCGGCAGGCCAATGGTGTTGTTGAGCAGTAAAAAGAAGc                                                     >  1:689800/1‑251 (MQ=255)

                                                                                                                                                                                               a‑GCACGCAGGCAAAAGCAAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCCATGCCCAGCGAC‑TGACACAACATACTGAAGCGGGCGGTGATATCGAGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGCTCGCCCTTAACGCATTGGTGAAACTCACACTGCCGTCAGCAGATTGCGGCAGGCCAATGGGGTTGTTGAGCAGGACAAAGAAGcc                                                    >  2:401714/1‑251 (MQ=255)

                                                                                                                                                                                                   aCGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACACAACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGCTCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGCGGCAGGCCAATGGTGATGTTGAGCAGTAAAAAGAAGCCggg                                                 >  1:139710/1‑250 (MQ=255)

                                                                                                                                                                                                     gcaggCAAAAGCCTGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACACAACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGCGGCAGGCCAATGGTGTTGTTGAGCAGTAAAAAGAAGCCGGTAg                                               <  2:257442/247‑1 (MQ=255)

                                                                                                                                                                                                       aggCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACACAACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGCGGCAGGCCAATGGTGTTGTTGAGCAGTAAAAAGAAGCCGGTAGcc                                             >  1:324869/3‑251 (MQ=255)

                                                                                                                                                                                                            aaagCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACACAACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGCGGCAGGCCAATGGTGTTGTTGAGCAGTAAAAAGAAGCCGGTAGCCAGcac                                        >  1:479044/4‑251 (MQ=255)

                                                                                                                                                                                                             aagCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACACAACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGCGGCAGGCCAATGGTGTTGTTGAGCAGTAAAAAGAAGCCGGTAGCCAGcac                                        >  1:906810/3‑250 (MQ=255)

                                                                                                                                                                                                              agCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACACAACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGCGGCAGGCCAATGGTGTTGTTGAGCAGTAAAAAGAAGCCGGTAGCCAGcaca                                       >  1:76379/2‑250 (MQ=255)

                                                                                                                                                                                                                  aGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACACAACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAGTCACAATGCCGTCAGCACATTGCGGCAGGCCAATGGTGTTGTTGAGCAGTAAAAAGAAGCCGGGAACCACCACaaatcc                                  >  2:321879/1‑247 (MQ=255)

                                                                                                                                                                                                                    cgcCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTACAATGCCCAGCGAC‑TGACACAACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGCGGCAGGCCAATGGTGTTGTTGAGCAGTAAAAATAAGCCGGTAGCCAGCACAATCCCCg                                <  1:705766/251‑1 (MQ=255)

                                                                                                                                                                                                                          cgcCAGTGGACGTTTCAGGGCGCGCAGCGCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACACAACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGCGGCAGGCCAATGGTGTTGTTGAGCAGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGCATgg                          <  2:245820/251‑1 (MQ=255)

                                                                                                                                                                                                                           gcCAGTGGACGTGTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACACAACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGCGGCAGGCCAATGGTGTTGTTGAGCAGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGCATGGc                         <  1:884806/251‑1 (MQ=255)

                                                                                                                                                                                                                             cAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACACAACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATAGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGCGGCAGGCCAATGGTGTTGTTGAGCAGTAAAAAGAAGCCGGGAGCAAGCACAATACCCGGCATgacg                        >  1:615284/1‑247 (MQ=255)

                                                                                                                                                                                                                               gTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACACAACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGCGGCAGGCCAATGGTGTTGTTGAGCAGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGCATGGCGAgg                     <  1:897131/251‑1 (MQ=255)

                                                                                                                                                                                                                                    cGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACACAACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGCGGCAGGCCAATGGTGTTGTTGAGCAGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCa                 >  1:690955/1‑250 (MQ=255)

                                                                                                                                                                                                                                       ttCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACTCAACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGCGGCAGGCCAATGGTGTTGTTGAGCAGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACa              <  1:125786/250‑1 (MQ=255)

                                                                                                                                                                                                                                       ttCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACACAACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGCGGCAGGCCAATGGTGTTGTTGAGCAGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACa              <  1:868964/250‑1 (MQ=255)

                                                                                                                                                                                                                                       ttCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCACAGCGAC‑TGACACAACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGCGGCAGGCCAATGGTGTTGTTGAGCAGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACAt             >  1:807414/1‑251 (MQ=255)

                                                                                                                                                                                                                                           gggCGCGCCGCTCGACTACTGTTAAGGAGGACCAGCCGTCAATGCCCA‑TGACATGACACAACATACTGTAGCGGGGGGGGATATCGCGCAGCGGGTTGTCCAGAGCGTTCAGCGCATAAGGGATCGCAATTAACGCAATGGGGAAAATCACAATGACGTCAGCAGGTTGAGGCAGGCCATTTGTGTTGTTGAGCAGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACATGcag         <  2:737438/251‑3 (MQ=255)

                                                                                                                                                                                                                                             cggcgaaGCTCGACCACTTTTCAGCGGGACCAGCCTCCAATGCCCAGCGAC‑TGACACAACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCCCAATGCCGTCAGCAGATTGCGGCAGGCCAATGGTGTTGTTGAGCAGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACATGCCGCt       <  2:419630/245‑1 (MQ=255)

                                                                                                                                                                                                                                                  cAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGAC‑TGACACAACATACTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGTACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGCGGCAGGCCAATGGTGTTGTTGAGCAGTAAAAAGAAGCCGGTAGCCCGCACAATCCCCGGCATGGCGGGGAGCAACATGCCGCCCAtct  >  2:42216/1‑251 (MQ=255)

AGTTTTAACATTTCGCCCCGGTAGTTTTTCAATCACGGTAAACAGCAGAAAACAGAGCAGCAGCAGAATTAACGCGGTGACCGCACCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACAACGCCACCACGCCAAAATCACCAATCG‑ACA‑GCACGCATGCAAAGGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCCACCACTTTTAAGCGTGACCAGCCTTCAATCCCCAGCGAC‑TGACATAACATGCTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGCACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGTGGCAGGCCGATAGTGTTGTTGAGCAGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACATGCCGCTCATCT  >  NC_000913/72917‑73407

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`