BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	73,001	A→G	G507G (GGT→GGC)	thiP ←	thiamine ABC transporter membrane subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	73,001	0	A	G	99.2%	342.9 / NA	119	G507G (GGT→GGC)	thiP	thiamine ABC transporter membrane subunit
Reads supporting (aligned to +/- strand): ref base A (1/0); new base G (58/59); total (60/59)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 119 total aligned reads displayed.

AGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAATTCAGCAGGGTACTTTTACCCGCGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCCACCGTTAAGCTAAAACGCATCGGCAAATGGTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATTTCGCCCCGGTAGTTTTTCAATCACGGTAAACAGCAGAAAACAGAGCAGCAGCAGAATTAACGCGGTGACCGCACCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACAACGCCACCACGCCAAAATCACCAATCGACA‑GCACGCATGCAAAGGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCCACCACTTTTAAGCGTGACCAGCCTTCAATCCCCAGCGACTGACATAACATGCTGTAGCGGGCGGTGATATCGCGCATCGGGTTT  >  NC_000913/72757‑73247

aGTCAGCGAACGGCTGGCTGGCGTCAGAAAACCGGCGAACAAGTTCAGCAGGGTACTTTTCCCTGCGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCTACCGTTAAGCTAAAACGCATCGGCAAATGTTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATCTCGCCCCGGTAGTTTTTCAATCACGGTAAACAGCAGAAAACAGAGTAGCAGCAGAATTAACGCGGTGACCGCGCCGTc                                                                                                                                                                                                                                                    <  2:660944/250‑1 (MQ=255)

     gCGAACCGCTGGCTGGAGTCAGAAAACCGGCGATCAAGTTCAGCAGGGTTCTTTTACCTGCGCCGCTTGGCCAGAGGATCGCCACCTGCTCGCCGCGTTCTACCGTTAAGCTAAAACGCATCGGCAAATGTTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATCGCGCCCCGGTAGTTTTTCAATCACGGTAAACAGCAGAAAACAGAGTAGCAGCAGAATTAACGCGGTGACCGCGCCGTTCTGGCt                                                                                                                                                                                                                                              <  1:384796/251‑1 (MQ=255)

      cGAACCGCAGGCGGGCGGCAGAAAACCGGCGATCAAGGTCAGCAGGGTACTTTTACCTGCGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCAGCGTTCTACCGTTAAGCTAAAACGCATCGGCAAATGTTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATCTCGCCCCGGTAGTTTTTCAATCACGGTAAACAGCAGAAAACAGAGTAGCAGCAGAATTAACGCGGTGACCGCGCCGTCCTGGCTg                                                                                                                                                                                                                                             <  2:139372/251‑1 (MQ=255)

        aaCCGCTGGCTGGCGTCAGAAAACCGGCGATAAAGTTCAGCAGGGTACTTTTACCTGCGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCTACCGTTAAGCTAAAACGCATCGGCAAATGTTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATCTCGCCCCGGTAGTTTTTCAATCACGGTAAACAGCAGAAACCAGAGTAGCAGCAGAATTAACGCGGTGACCGCCCCGTCCTGGCTGCg                                                                                                                                                                                                                                           >  2:985207/1‑251 (MQ=255)

           cggtggccggcGTCAGAAAACCGGCGAGCAAGTGCAGCAGGGTACTTTTACCCGCGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCTACCGTTAAGCTAAAACGCATCGGCAAATGTTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATCTCGCCCCGGTAGTTTTGCAATCACGGTAAACAGCAGAAAACAGAGTAGCAGCAGAATTAACGCGGTGACCGCGCCGTCCTGGCTGCGATa                                                                                                                                                                                                                                        <  2:672346/248‑1 (MQ=255)

            gctggctgGCGTCAGAAAACCGGCGATCAAGTTCAGCAGGGTACTTTTACCTGCGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCTACCGTTAAGCTAAAACGCATCGGCAAATGTTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATCTCGCCCCGGTAGTTTTTCAATCACGGTAAACAGCAGAAAACAGAGTAGCAGCAGAATTAACGCGGTGACCGCGCCGTCCTGGCTGCGATAg                                                                                                                                                                                                                                       <  1:712461/251‑1 (MQ=255)

             ctggctggCGTCAGAAAACCGGCGATCAAGTTCAGCAGGGTACTTTTACCTGCGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCTACCGTTAAGCTAAAACGCATCGGCAAATGTTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATCTCGCCCCGGTAGTTTTTCAATCACGGTAAACAGCAGAAAACAGAGTAGCAGCAGAATTAACGCGGTGACCGCGCCGTCCTGGCTGCGATAgg                                                                                                                                                                                                                                      >  1:122646/1‑251 (MQ=255)

               tgctggcGTCAGAAAACCGGCGATCAAGTTCAGCATGGTACTTTTCCCTGCTCCGCTTTGCCCGAGGATCGCCACCTGCTCGCTGCGTTCTACCGTTAAGCTAAAACTCATCGGCAAATGTTGGTAAAGCCAGGGGATATCAGTCAGTTTTAACATCGCGCCCCGGTAGTTTTTCAATCACGGTAAACAGCAGAAAACAGAGTAGCAGCAGAAGTAACGCGGTGACCGCGCCGTCCTGGCTGCGATatga                                                                                                                                                                                                                                     <  2:599934/249‑4 (MQ=255)

                   ggcGTCAGAAAACCGGCGATCAAGTTCAGCAGGGTACTTTTACCTGCGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCTACCGTTAAGCTAAAACGCATCGGCAAATGTTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATCTCGCCCCGGTAGTTTTTCAATCACGGTAAACAGCAGAAAACAGAGTAGCAGCAGAATTAACGCGGTGACCGCGCCGTCCTGGCTGCGATAGGAGCCa                                                                                                                                                                                                                                 <  1:910172/250‑1 (MQ=255)

                    gcGTCAGAAAACCGGCGATCAAGTTCAGCAGGGTCCTTTTACCTGCGCCGCTTGGCCGGAGGATCGCCGCCGGCTCGCCGCGTTCTACCGTTAAGCTAAAACGCATCGGCAAATGTTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATCTCGCCCCGGTAGTTTTTCAATCACGGTAAACAGCAGAAAACAGAGTAGCAGCAGAATTAACGCGGTGACCGCGCCGTCCTGGCTGCGATAGGAGCCAAt                                                                                                                                                                                                                               <  1:703931/251‑1 (MQ=255)

                          gAAAACCGGCGATCAAGTTCAGCAGGGTACTTTTACCTGCGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCTACCGTTAAGCTAAAACGCATCGGCAAATGTTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATCTCGCCCCGGGATTTTTTCAATCACGTTAAACAGCAGAAAACAGAGTAGCAGCAGAATTAACGCGGTGACCGCGCCGTCCTGGCTGCGATAGGAGCCAATTTGCTg                                                                                                                                                                                                                         <  1:432302/251‑1 (MQ=255)

                           aaaaCCGGCGATCAAGTTCAGCAGGGTACTTTTACCTGCGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCTACCGTTAAGCTAAAACGCATCGGCAAATGTTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATCTCGCCCCGGTAGTTTTTCAATCACGGTAAACAGCAGAAAACAGAGTAGCAGCAGAATTAACGCGGTAGCCGCGCCGTCCTGGCTGCGTTAGGAGCCAACTTGCTgg                                                                                                                                                                                                                        >  2:776103/1‑251 (MQ=255)

                             aaCCGGCGAGCAAGTTCAGCAGGGTACTGTTACCTGCGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCTACCGTTAAGCTAAAACGCATCGGCAAATGTTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATCTCGCCCCGGTAGTTTTTCAATCACGGTAAACAGCAGAAAACAGAGTAGCAGCAGAATTAACGCGGTGACCGCGCCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTa                                                                                                                                                                                                                      <  1:449732/251‑1 (MQ=255)

                               ccggagtgcaatttCAGCAGGTTACTTTTCCCTGCGTCGCTTGGACCGAGGATCGCCACCTGCTCGCCGCGTTCTAACGTTAAGCTAAACCGCATCGGCAAATGTTGGTAAAGCCATGTGATATCAGTCAGTTTTAACATCTCGCCCCGGTAGTTTTTCAATCACGGTAAACAGCAGAAACCAGAGTAGCAGCAGAATTAACGCGGTGACCGCGCCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTaga                                                                                                                                                                                                                    <  2:277324/239‑1 (MQ=255)

                                        aagttCAGCAGGGTACTTTTACCTGCGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCTACCGTTAAGCTAAAACGCATCGGCAAATGATGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATCTCGCCCCGGTAGTTTTTCAATCACGGTAAACAGCAGAAAACAGAGTAGCAGCAGAATTAACGCGGTGACCGCGCCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACg                                                                                                                                                                                                           >  1:358735/4‑251 (MQ=255)

                                            tCAGCAGGGTACTTTTACCTGCGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCTACCGTTAAGCTAAAACGCATCGGCAAATGTTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATCTCGCCCCGGTAGTTTTTCAATCACGGTAAACAGCAGAAAACAGAGTAGCAGCAGAATTAACGCGGTGACCGCGCCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCa                                                                                                                                                                                                        <  1:505817/250‑1 (MQ=255)

                                             cagcagGGTACTTTTACCTGTGCCGCTTGGCCCGAGGATCGCCCCCTGCTCGCCGTGTTCGACCGTGAAGCTAAAACGCATCGGCAAATGTTGGTAAAGCCAGTTGATATCAGTCAGTTTTAACATCTCGCCCCGGTAGTTTTTCAATCACGGTAAACAGCAGAAAACAGAGTAGCAGCAGAATTATCGCGGTGACCGCGCCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAg                                                                                                                                                                                                       <  2:751007/250‑1 (MQ=255)

                                             cagcagGGTACTTTTACCTGCGCCGCTTGGCACGAGGATCGCCATCTGCTCGCCGCGATCTTCCGTTAAGCTAAAACGCATCGGCAAATTTTGGTAAAGCCAGGGGATATCAGTCAGTTTTAACATCTCGCCCCGGTAGTTTTTCAATCACGGTAAACAGCAGAAAACAGAGTAGCAGCAGAATTAACGCGGTGACCGCGCCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAgg                                                                                                                                                                                                      <  1:686216/251‑1 (MQ=255)

                                                 ggggtccttctccctgcgcCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCTACCGTTAAGCTAAAACGCATCGGCAAATGTTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATCTCGCCCCGGTAGTTTTTCAATCACGGTAACCAGCAGAAAACAGAGTAGCAGCAGAATTAACGCGGTGACCGCGCCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGttc                                                                                                                                                                                                  <  2:242983/236‑3 (MQ=255)

                                                      aCTTTTACCTGCGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCTACCGTTAAGCTAAAACGCATCGGCAAATGTTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATCTCGCCCCGGTAGTTTTTCAATCACGGTAAACAGCAGAAAACAGAGTAGCAGCAGAATTAACGCGGTGACCGCGCCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGaaa                                                                                                                                                                                             <  1:93437/251‑1 (MQ=255)

                                                       cTTTTACCTGCGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCTACAGTTAAGCTAATACGCATCGGCAAATGTTGGTAAAGCCAGGCGATAACAGTCAGTTTTAACATCTCGCCCCGGTAGTTTTTCAATCACGGTAAACAGCAGAAAACAGGGTAGCAGCAGAATTAACGCGGTGACCGCGCCGTCCTGGCTGCGCTAGGAGCCAATTAGCTGGTAGAGATAAAACGGCAGGGGGCGGaca                                                                                                                                                                                             >  2:379146/1‑248 (MQ=255)

                                                       cTTTTACCTGCGCCGCTCGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCTACCGTTAAGCTAAAACGCATCGGCAACTGTTGGTAAAGCAAGGTGATATCAGTCAGTTTTAACATCTCGCCCCGGTAGTTTTTCAATCACGGTAAACAGCAGAAAACAGAGTAGCAGCAGAATTAACGCGGTGAACGCGCGGCCCTGGCTGCGAAAGGACCCAATTTGCTGGATGAGATAAAACGGCAGGGTGCgggaa                                                                                                                                                                                             >  2:504572/1‑247 (MQ=255)

                                                         tttACCTGCGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCTACCGTTAAGCTAAAACGCATCGGCAAATGTTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATCTCGCCCCGGTAGTTTTTCAATCACGGTAAACAGCAGAAAACAGAGTAGCAGCAGAATTAACGCGGTGACCGCGCCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAatc                                                                                                                                                                                           >  1:782198/1‑250 (MQ=255)

                                                         tttACCTGCGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCTACCGTTAAGCTAAAACGCATCGGCAAATGTTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATCTCGCCCCGGTAGTTTTTCAATCACGGAAAACAGCAGAAACCAGAGTAGCCGCAGAATTCACGCGGTGCCCGCGCCGTCCTAGCTGCGAGAGGAGCCAATCTGCTGGTAGAGAGAAAATGGCAGGGCGCGCAAata                                                                                                                                                                                           >  2:776457/1‑249 (MQ=255)

                                                            acctgcgcCGCTTGGCCCGAGGATCGCCACCTGCACGCCGCGTTCTACCGTTAAGCTAAAACGCATCGGCAAATGTTGGTAAAGCCACGTGATATCAGTCAGTTTTAACATCTAGCCCCGGTAGTTTTTCAAACACGGTAAACAGCAGTAAACAGCGTAGCAGCAGAATCAACGAGGTGACCGCACCGACTTTGCTGCGATAGGAGCCAATTGGATGGTAGAGATAAAACGGCAGAGGGCGGAAatctt                                                                                                                                                                                         >  2:614265/5‑247 (MQ=255)

                                                             cctgcgcCGCTTGGCCCGAGGATCGCCACCTGCTAGCCGCGTTCTACCGTTAAGCTAAAACGCATCGGCAAATGTTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATCTCGCCCCGGTAGTTTTTCAATCACGGTAAACAGCAGAAAACAGAGTAGCAGCAGAATTAACGCGGTGACCGCGCCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGt                                                                                                                                                                                      >  1:109604/4‑251 (MQ=255)

                                                                     gcTTGGACCGAGGATCGCCACCTGCTCGCCGCGTTCTACCGTTAAGCTAAAACGCATCGGCAAATGTTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATCTCGCCCCGGTAGTTTTTCAATCACGGTAAACAGCAGAAAACAGAGTAGCAGCAGAATTAACGCGGTGACCGCGCCGTCCTGGCTGCGATAGGAGCAAATTTGCTGGAAGAGATAAAACGGCAGGGGGCGGGAATCATCGTTACAGaa                                                                                                                                                                               >  1:522693/1‑250 (MQ=255)

                                                                        gggCCCGAGGATCGCCACCTGCTCGCCGCGTTCTACCGTTAAGCTAAAACGCATCGGCAAATGTTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACACCTCGCCCCGGTAGTTTTTCAATCACGGTAAACAGCAGAAAACAGAGTAGCAGCAGAATTAACGCGGTGACCGCGCCGTCCTGGCTGCGATAGTAGCCAATCTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCAGCGTTACCGaacaac                                                                                                                                                                           <  1:133372/250‑1 (MQ=255)

                                                                         ggCCCGAGGATCGCCACCGGCGCGCCGCGTTCTACCGTTAAGCTAAAACGCATCGGCAAATGTTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATCTCGCCCCGGTAGTTTTTCAATCACGGTAAACAGCAGAAAACAGAGTAGCAGCAGAATTAACGCGGTGACCGCGCCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGTAAATCAGCGTTACCGAACAACg                                                                                                                                                                          <  2:147182/251‑1 (MQ=255)

                                                                           cccGAGGATCGAAACCTGCTAGCCGCGTGCTACCGCTAAGCAAAAAAGCATCGGCAAGTGTTGGACAAGCCAGGAGCTATAAGTCAGTTTTAACATCTCGCCCAGGAAGCTTTTCAATCACGGTAAACAGCAGAAAACAGAGTAGCAACAGACTTCACGCGGATACCGCGCAGTCCTTGCTGTGATAGGAGCCCCTTTGCTGGTAGAGCTAGACCCGCAGGGGTCGGAAAACAACGTCACCGAACAACGcc                                                                                                                                                                        >  2:493406/1‑251 (MQ=255)

                                                                             cGAGGATCGCCACCTGCTCGCCGCGTTCTACCGTTAAGCTAAAACGCATCGGCAAATGTTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATCTCGCCCCGGTAGTTTTTCAATCACGGTAAACAGCAGAAAACAGAGTAGCCGCAGAATTAACGCGGTGACCGCGCCGTCCTGGCTGCGGTAGGAGCCAATTTGCTGGTAGAGATAACACGGCAGGGGGCGGAAATCATCGTTACCGAACAACGcccc                                                                                                                                                                      >  2:784634/1‑249 (MQ=255)

                                                                              gctgatcggtcacTGCTCACCGCCTTCGACCGTTAAGCTAAAACGCACCGGCAAACGTTGGTAAAGCCAGTTGATATCAGGCAGTTTTACAATCTCCGCCCGGTAGTTTTTTAATCACTGTAAACAGCTGAAAACAGAGGGGCAGCAGAATTAACGCGGTGACCGCGCCGTTCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATACAACGGCAGTGTGCGGATATCATCGTTACCGACCAACGcaatt                                                                                                                                                                     <  2:671123/239‑5 (MQ=255)

                                                                              gAGGATCGCCACCTGCTCGCCGCGTTCTACCGTTAAGCTAAAACGCATCGGCAAATGTTGGTAAAGCCAGGAGATATCAGTCAGTTTTAACATCTCGCCCCGGTAGTTTTTCAATCACGGTACACAGCAGAAAACAGAGTAGAAGCAGACTTAACGCGGTGACCGCGCCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACAACGccacc                                                                                                                                                                     >  1:887249/1‑251 (MQ=255)

                                                                               aGGATCGCCACCAGCTCGCCGCGTTCTACCGTTAAGCTAAAACGCATCGGCAAATGTTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATCTCGCCCCGGTAGTTTTTCAATCACGGTAAACAGCAGAAAACAGAGTAGCAGCAGAATTAACGCGGTGACCGCGCCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACAACGccacca                                                                                                                                                                    <  2:591522/251‑1 (MQ=255)

                                                                                ggatggccccctgcatgccgcGTTCGACCGTTAAGCTAAAACGCATCGGCAAATGTTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATCTCGCCCCGGTAGTTTTTCAATCACGGTAAACAGCAGAAAACAGAGTAGCAGCAGAATTAACGCGGTGACCGCGCCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACAACGCcaccac                                                                                                                                                                   <  2:19153/235‑1 (MQ=255)

                                                                                ggATCGCCACCTGCTCGCCGCGTTCTACCGTTAAGCTAACACGCATCGGCCAATGTTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATCTCGCCCCGGTAGTTTTTCAATCACGGTAAACAGCAGAAAACAGAGTAGCAGCAGAATTAACGCGGTGACCGCGCCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACAACGCcaccac                                                                                                                                                                   >  1:374965/1‑251 (MQ=255)

                                                                                      ccACCTGCTCGCCGCGTTCTACCGTTAAGCTAAAACGCATCGGCAAATGTTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATCTCGCCCCGGTAGTTTTTCAATCACGGTAAACAGCAGAAAACAGAGTAGCAGCAGAATTAACGCGGTGACCGCGCCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACAACGCCACCACGCCaaa                                                                                                                                                             <  1:697980/251‑1 (MQ=255)

                                                                                        aCCTGCTCGCCGCGTTCTACCGTTAAGCTAAAACGCATCGGCAAATGTTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATCTCGCCCCGGTAGTTTTTCAATCACGGTAAACAGCAGAAAACAGAGTAGCAGCAGAATTAACGCGGTGACCGCGCCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACAACGCCACCACGCCAAAAt                                                                                                                                                           <  1:48590/251‑1 (MQ=255)

                                                                                            gCTCGCCGCGTTCTACCGTTAAGCTAAAACGCATCGGCAAATGTTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATCTCGCCCCGGTAGTTTTTCAATCACGGTAAACAGCAGAAAACAGAGTAGCAGCAGAATTAACGCGGTGACCGCGCCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGGGCGGAAATCATCGTTACCGAACAACGCCACCACGCCAAAATCAc                                                                                                                                                        >  2:703281/1‑250 (MQ=255)

                                                                                                   ccgttctcacGTTAAGCTAAAACTCATCCGCAAACGTTGGTAAATCCCGGTGATATCAGCTAGTTTTAACATCTCGCCCCGGTAGGTTTTCAATCACGGTAAACAGCAGAACACAGAGTAGCAGCAGAATTAACGCGGTGACCGCGCCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACAACGCCACCTCGCCAAAATCACCAATCGAc                                                                                                                                                <  2:754285/242‑1 (MQ=255)

                                                                                                     gTTCTACCGTAAAGCTAAAACGCATCGGCAAATGTTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATCTCGCCCCGGTAGTTTTTCAATCACGGTAAACAGCAGAAAACAGAGTAGCAGCAGAATTAACGCGGTGACCGCGCCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACAACGCCACCACGCCAAAATCACCAATCGAc                                                                                                                                                >  1:187090/1‑249 (MQ=255)

                                                                                                       tctaCCGTTAAGCTAAAACGCCTCGGCAAATGTTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATCTAGCCCCGGTAGTTTTTCAATACCGGGAAACAGCAGAAAACAGAGTAGCAGCAGAATTAACGCGGTGACCGCGCCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGGGCGGGAATCATCGTTACCGAACAACGCAACCACGCCAAAATCACCAATCGACA‑Gcc                                                                                                                                           >  2:822751/4‑250 (MQ=255)

                                                                                                             gTTAAGCTAAAACGCATCGGCAAATATTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATCTCGCCCCGGAAGTTTTTCAATCACGGTAAACAGCAGAAAACAGCGTAGCAGCAGAATTAACGCGGTGACCTCGCCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCTGAGTGCGAGAATACTCGTTACCGAACAACGCCACCACGACAAAAACAACAATCGaatccacgcagg                                                                                                                                      >  2:663097/1‑240 (MQ=255)

                                                                                                                 aGCTAAAACGCATCGGCAAATGTGGGTAAAGCCAGGTGATATCAGTCAGGTTTAACATCTCGCCCCGGTAGTTTTTCAATCACGGTAAACAGCAGAAAACAGAGTAGCAGCAGAATTAACGCGGTGACCGCGCCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACAACGCCACCACGCCAAAATCACCAATCGACA‑GCACGCAGGCaaa                                                                                                                                 <  2:311053/251‑1 (MQ=255)

                                                                                                                   cTAAAACGCATCGGCAAATGTTGGTAAAGCCCGGTGATATCAGTCAGTTTTAACATCTCGCCCCGGTAGTTTTTCAATCACGGTAAACAGCAGAAAACAGAGTAGCAGCAGAATTAACGCGGTGACCGCGCCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACAACGCCACCACGCCAAAATCACCAATCGACA‑GCACGccggcaaa                                                                                                                                 >  1:756129/1‑242 (MQ=255)

                                                                                                                    tAAAACGCATCGGCAAATGTTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATCTCGCCCCGGTAGTTTTTCAATCACGGTAAACAGCAGAAAACAGAGTAGCAGCAGAATTAACGCGGTGACCGCGCCGTCCTGGCTGCGATATGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACAACGCCACCACGCCAAAATCACCAATCGACA‑GCACGCAGGCaaaagc                                                                                                                              >  1:887357/1‑248 (MQ=255)

                                                                                                                    tAAAACGCATCGGCAAATGTTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATCTCGCCCCGGTAGTTTTTCAATCACGGAAAACAGCAGAAAACAGAGTAGCAGCAGAATTAACGCGGTGACCGCGCCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACAACGCCACCACGCCAAAATCACCACTCGACA‑GCACGCAGGCaaaagc                                                                                                                              >  1:366806/1‑248 (MQ=255)

                                                                                                                     aaaaCGCATCGGCAAATGTTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATCTCGCCCCGGTAGTTTTTCAATCACGGTAAACAGCAGAAAACAGAGTAGCAGCAGAATTAACGCGGTGACCGCGCCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACAACGCCACCACGCCAAAATCACCAATCGACA‑GCACGCAGGCAAAAGcc                                                                                                                             >  1:752537/1‑251 (MQ=255)

                                                                                                                      aaaCGCATCGGCAAATGTTGGTAAAGCCCGGAGATATCAGTCAGTTTTAACCTCTCGCCCCGGTAGTTTTTCAATCACGGTAAACAGCAGAAAACAGAGTAGCAGCAGAATTAACGCGGTGACCGCGCCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGGTCAAACGGCAGGGAGCGGCAATCATCGTTCCCGAACAACGCCACCACGCCACAATCACCAATCGACA‑GCACGCAGGCaaaaacc                                                                                                                             >  2:705766/1‑246 (MQ=255)

                                                                                                                      aaaCGCATCGGCAAATGTTGGGAAAGCCAGGTGATATCAGTCAGGTTTAACATCTCGCCCCGGTAGTTTGTCAATCACGGTAAACAGCAGAAAACAGAGTAGCAGCAGAATTAACGCGGTGACCGCGCCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACAACGCCACCACGCCAAAATCACCAATCGACA‑GCACGCAGGCAAAAGCCa                                                                                                                            <  2:195512/251‑1 (MQ=255)

                                                                                                                       aaCGCATCGGCAAATGTTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATCTCGCCCCGGTAGTTTTTCAATCACGGTAAACAGCAGAAAACAGAGTAGCAGCAGAATTAACGCGGTGACCGCGCCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCCTCGTTACCGAACAACGCCACCACGCAAAAATCACCAATCG‑CACGCACGCAGGCaaaaacac                                                                                                                            >  2:868964/1‑245 (MQ=255)

                                                                                                                       aaCGCATCGGCAAATGTTGGTAAAGACAGGTGATATCAGTCAGTTTTAACATCTCGCCCAGGTAGTTTTTCAATCACGGTAAACAGCAGAAAACAGAGTAGCAGCAGAATTAACGCGGTGACCGCGCCATCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGTTAAAACGGCAGGGTGAGGAAATCATCGTTACCGAACAAAGCCACCACGACAAAATCACCAAACGAAA‑ACACGccggaaaaagcca                                                                                                                            >  2:342550/1‑238 (MQ=255)

                                                                                                                                   aaTGTTGGTAAAGACAGGTGATATCAGTCAGTTTTAACATCTCGCCCCGGTAGTTTTTCAATCACGGTAAACAGCAGAAAACAGAGAAGCAGCAGAATTAACGCGGTGACCGCGCCGTCATGGCTGCGATCGGAGCCAATTTGCTGGTAGCGATAAACCGGCAGGATGCGGAAATCATCGTTACCGCACAAAGCACCCACGCCAAAATCACAACACGACA‑GCACGAAGGCAAAAGCCacacccagcccccg                                                                                                               >  2:928198/1‑238 (MQ=255)

                                                                                                                                    agtttgggaaaGCCAGGTGATATCAGTCAGGTTAAACATCTCGCCCCGGTAGTTATTCAAGCACGGGAAACAGCAGAAAACAGAGGAGCAGCAGAATTAACGCGGTGACCGCGCCGTCCTGGCTGCGATAGGAGCCAATTTGCGGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACAACGCCACCACGCCAAAATCACCAATCGACA‑GCACGCTGGCAAAAGCCTGCGCCTgagcaagt                                                                                                              <  2:986626/243‑8 (MQ=255)

                                                                                                                                      gttggtAAAGCCAGGTGATATCAGTCAGTTTTAACATCTCGCCCCGGTAGTTTTTCAATCACGGTAAACAGCAGAAAACAGAGTAGCAGCAGAATTAACGCGGTGACCGCGCCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGGGCGGAAATCATCGTTACCGAACAACGCCACCACGCCAAAATCACCAATCGACA‑GCACGCAGGCAAAAGCCAGCGCCCGCCCCAGTgg                                                                                                            >  2:295953/3‑251 (MQ=255)

                                                                                                                                         ggtAAAGCCAGGTGATATCAGGCAGTTTTAACATCTCGCCCCGGGAGTTTTTCAATCACGGTAAACAGCAGAAAACAGAGTAGCAGCAGAATGAACGCGGTGACCGCGCCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACAACGCCACCACGCCAAAATCACCAATCGACA‑GCACGCAGGCAAAATCCAGCGCCTGCGCCAGTGGAc                                                                                                          <  2:160748/250‑1 (MQ=255)

                                                                                                                                            aaaGCCAGGTGATATCAGTCAGTTTTAACATCTCGCCCCGGTAGTTTTTCAATCACGGTAAACAGCAGAAAACAGAGTAGCAGCAGAATTAACGCGGTGACCGCGCCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACAACGCCACCACGCCAAAATCACCAATCGACA‑GCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGttt                                                                                                      <  1:819980/251‑1 (MQ=255)

                                                                                                                                             aaGCCAGGTGATATCAGTCAGTTTTAACATCTCGCCCCGGTAGTTTTTCAATCACGGTAAACAGCAGAAAACAGAGTAGCAGCAGAATTAACGCGGTGACCGCGCCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACAACGCCACCACGCCAAAATCACCAATCGACA‑GCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTc                                                                                                     >  1:367893/1‑251 (MQ=255)

                                                                                                                                              agtcAGGAGATGTCAGTCAGCTTTAACATCTCGCCCCGGTAGTTTTTCAATCCCGGTAAACAGCAGAAAACAGAGTAGCAGCAGAATTAACGCGGTGACTGCGCCGTCCTGGCTGTGATATGAGCCAATTTGCTGTTCGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACAACGCCACCACGCCAAAATCACCAATCGACA‑GCACGCATGCAACAGCCAGCGCCTGTGCCAGTGGTCGTTTCa                                                                                                    <  2:880136/248‑1 (MQ=255)

                                                                                                                                               gCCAGGTGATATCAGTCAGTTTTAACATCTCGCCCCGGTAGTTTTTCAATCACGGTAAACAGCAGAAAACAGAGTAGCAGCAGAATTAACGCGGTGACCGCGCCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACAACGCCACCACGCCAAAATCACCAATCGACA‑GCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTc                                                                                                     >  1:104848/1‑249 (MQ=255)

                                                                                                                                                ccAGGTGATATCAGTCAGTTTTAACATCTCGCCCCGGTAGTTTTTCAATCACGGTAAACAGCAGAAAACAGAGTAGCAGCAGAATTAACGCGGTGACCGCGCCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACAACGCCACCACGCCAACATCACCAATCGACA‑GCACGCAGGCAAAAGCCAGCGCCTGCGCCAGGGGCCGttttcgg                                                                                                  >  2:218672/1‑247 (MQ=255)

                                                                                                                                                 cAGGTGATATCAGTCAGTTTTAACATCTCGCCCCGGTAGTTTTTCAATCACGGTAAACAGCAGAAAACAGAGTAGCAGCAGAATTAACGCGGTGACCGCGCCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACAACGCCACCACGCCAAAATCACCAATCGACA‑GCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAggg                                                                                                 <  1:629233/251‑1 (MQ=255)

                                                                                                                                                   ggTGATATCAGTCAGTTTTAACAGCTCGCCCCGGTAGTTTTTCAATCACGGTAAACAGCAGAAAACAGAGTAGCAGCAGAATTAACGCGGTGACCGCGCCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACAACGCCACCACGCCAAAATCACCAATCGACA‑GCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGgcg                                                                                               <  1:570215/251‑1 (MQ=255)

                                                                                                                                                     tGATATCAGTCAGTTTTAACATCTCGCCCCGGTAGTTTTTCAATCACGGTAAACAGCAGAAAACAGAGTAGCAGCAGAATTAACGCGGTGACCGCGCCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGAGCGGAAATAATCGTTAACGAACAACGCCACCAAGCCAAAATCACCAATCGAAA‑GCACGCAGGCAAAAGCCAGCGCCTGCGCCCGTGGCCGTTTCAGGgcgcg                                                                                             >  2:500274/1‑251 (MQ=255)

                                                                                                                                                           cagtcagtTTTAACATCTCGCCCCGGTAGTTTTTCAATCACGGTAACCAGCAGAAAACAGAGTAGCCGCAGAATTAACGCGGTGACCGCGCCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGCACAACGCCACCACGCCAAAATCACCAATCGCCA‑GCACGCCGGCAAAAGCCAGCGCCTGCGCCCGTGGACGTTTCAGGGCGCGCAGCTc                                                                                       >  2:897131/1‑251 (MQ=255)

                                                                                                                                                           cagccagtTGTAACATCACGCCCCGTAAGGTTTTCAAGCACGGTAAACAGCAGAAAACAGAGTAGCAGCAGAATTAACGCGGTGACCGGGCCGTCCTGGCGGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACAACGCCACCACGCCCAAATCACCAAGCGACA‑GCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTc                                                                                       <  2:651348/247‑1 (MQ=255)

                                                                                                                                                            agtcagtTTTAACATCTCGCCCCGGTAGTTTTTCAATCACGGTAAACAGCAGAAAACAGAGTAGCAGCAGAATTAACGCGGTGACCGCGCCGTCCTGGCTGCGATAGTAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACAACGCCACCACGCCAAAATCACCAATCGACA‑GCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTcg                                                                                      >  1:834849/1‑250 (MQ=255)

                                                                                                                                                            agtcagtTTTAACATCTCGCCCCGGTAGTGTTTCAATCACGGTAAACAGCAGAAAACAGAGTAGCAGCAGAATTAACGCGGTGACCGCGCCGCCCTGGCTGCGAGAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACAACGCCACCACGCCAAAATCACCAATCGACA‑GCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCtag                                                                                      <  2:801877/251‑3 (MQ=255)

                                                                                                                                                            agtcagtGTTAACAACTCGCCCCTGTAGGTTTTCAATCAAGGTAAACAGTAGAAAACAGAGTAGCAGCAGAATTAACGCGGTGACCGCGCCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACAACGCCACCACGCTAAAATCACCAATCGACA‑GCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTcg                                                                                      <  2:845781/251‑2 (MQ=255)

                                                                                                                                                                agtTTTAACATCTCGCCCCGGTAGTTTTTCAATCACGGTAAACAGCAGAAAACAGAGTAGCAGCAGAATTAACGCGGTGACCGCGCCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACAACGCCACCACGCCAAAATCACCAATCGACA‑GCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTcgacca                                                                                  <  1:181801/251‑1 (MQ=255)

                                                                                                                                                                 gtTTTAACATCTCGCCCCGGTAGTTTTTCAATCACGGTAAACAGCAGAAAACAGAGTAGCAGCAGAATTAACGCGGTGACCGCGCCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACAACGCCACCACGCCAAAATCACCAATCGACA‑GCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCgaccac                                                                                 <  1:703281/251‑1 (MQ=255)

                                                                                                                                                                    ttAACATCTCGCCCCGGTAGTTTTTCAATCACGGTAAACAGCAGAAAACAGAGTAGCAGCAGAATTAACGCGGTGACCGCGCCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACAACGCCACCACGCCAAAATCACCAATCGACA‑GCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACttt                                                                              >  1:495484/1‑251 (MQ=255)

                                                                                                                                                                         aactCGCCCCGGTAGTTTTTCAATCACGGTAAACAGCAGAAAACAGAGTAGCAGCAGAATTAACGCGGGGACCGCGCCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACAACGCCACCACGCCAAAATCACCAATCGACA‑GCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGc                                                                         <  2:321959/248‑1 (MQ=255)

                                                                                                                                                                                cccGGTAGTTTTTCAACCACGGTAAACAGCAGAAAACAGAGGAGCAGCAGAATTAACGCGGTGACCGCGCCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACTGCAGGGTGCGGAAATCATCGTTACCGAACAACGCCACCACTCCAAAATCACCAATCGACA‑GCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCa                                                                  <  2:337507/251‑1 (MQ=255)

                                                                                                                                                                                 ccGGTAGTTTTTCAATCACGGTAAACAGCAGAAAACAGAGTAGCAGCAGAATTAACGCGGTGACCGCGCCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACAACGCCACCACGCCAAAATCACCAATCGACA‑GCACGCAGGCAAAAGCCAGAGCCTGAGCCAGTGGACGTTTCAGGGCGCGCAGCTCGCCCACTTTTAAGCGCGAcc                                                                   >  2:278462/1‑249 (MQ=255)

                                                                                                                                                                                  cGGTAGTTTTTCAATCACGGTAAACAGCAGAAAACAGAGTAGCAGCAGAATTAACGCGGTGACCGCGCCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACAACGCCACCACGCCAAAATCACCAATCGACA‑GCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGc                                                                >  1:41143/1‑251 (MQ=255)

                                                                                                                                                                                       gTTTTTCAATCACGGTAAACAGCAGAAAACAGAGTAGCAGCAGAATTAACGCGGTGACCGCGCCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACAACGCCACCACGCCAAAATCACCAATCGACA‑GCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTc                                                            >  1:412301/1‑250 (MQ=255)

                                                                                                                                                                                         ttttCAATCACGGTAAACAGCAGAAAACAGAGTAGCAGCAGAATTAACGCGGTGACCGCGCCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACAACGCCACCACGCCAAAATCACCAATCGACA‑GCACGCAGGCAAAAGCCACCGCCTGCGCCAGGGGACGTTTCAGGGCGCGCAGCTCGACCCCTTTTAAGCGCGACCAGCCTTCAAt                                                         >  2:529987/1‑251 (MQ=255)

                                                                                                                                                                                              aaTCACGGTAAACAGCATAAAACAGAGTAGCAGCCGAATTAACGCGGTGACCGCGCCGTCCTGGCTGCGATAGGAGCAAATTGTCTGGGAGAGATAAAACGGCAGGGTGCGGAAATCATCGGTACCGAACAACGCCACCACGCCAAAATCACCAAACGACT‑GCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTGTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTGCAATGCCCa                                                    <  2:97242/251‑1 (MQ=255)

                                                                                                                                                                                              aaTCACGGTAAACAGCAGAAAACAGAGTAGCAGCAGAATTAACGCGGTGACCGCGCCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACAACGCCACCACGCCAAAATCACCAATCGACA‑GCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATgccc                                                     >  1:69178/1‑250 (MQ=255)

                                                                                                                                                                                               aTCACGGTAAACAGCAGAAAACAGAGTAGCAGCAGAATTAACGCGGTGACCGCGCCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACAACGCCACCACGCCAAAATCACCAATCGACA‑GCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAACGCGACCAGCCTTCAATgccc                                                     >  1:146537/1‑249 (MQ=255)

                                                                                                                                                                                                tCACGGTAAACAGCAGAAAACAGAGTAGCAGCAGAATTAACGCGGTGACCGCGCCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACAACGCCACCACGCCAAAATCACCAATCGACA‑GCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGc                                                  >  2:747418/1‑251 (MQ=255)

                                                                                                                                                                                                  aCGGTAAACAGCAGAAAACAGAGTAGCAGCAGAATTAACGCGGTGACCGCGCCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACAACGCCACCACGCCAAAATCACCAATCGACA‑GCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCACTGCCCAGCgc                                                >  2:873943/1‑250 (MQ=255)

                                                                                                                                                                                                       aaaCAGCAGAAACCAGAGTAGCAGCAGCATTAACGCGGTGACCGCGCCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACAACGCCACCACGCCAAAATCACCAATCGACA‑GCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCCCGACCAGACTTCACTGCCCAGCGACTgcc                                           >  2:52521/1‑249 (MQ=255)

                                                                                                                                                                                                       aaaCAGCAGAAAACAGAGTAGCAGCAGAATTAACGCGGTGACCGCGCCGTCCTGGTTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAAGCATCGTTACCGAACAACGCCACCACGCCAAAATCGCCAATCGACA‑GCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGTCGTTTCAGGGCGCGCAGCTCGACCTCTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGACTGa                                            <  2:892790/250‑1 (MQ=255)

                                                                                                                                                                                                        aaCAGCAGAAAACAGAGTAGCAGCAGAATTAACGCGGTGACCGCGCCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACAACGCCACCACGCCAAAATCACCAATCGACA‑GCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGACTGACa                                          <  1:489696/251‑1 (MQ=255)

                                                                                                                                                                                                        aaCAGCAGAAAACAGAGTAGCAGCAGAATTAACGCGGTGACCGCGCCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACAACGCCACCACGCCAAAATCACCAATCGACA‑GCACGCAGGCAAAAGCCAGCGCATGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCgcatgacc                                          >  2:624460/1‑244 (MQ=255)

                                                                                                                                                                                                         aCAGCAGAAAACAGAGTAGCAGCAGAATTAACGCGGTGACCGCGCCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACAACGCCACCACGCCAAAATCACCAATCGACA‑GCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCCCGACCCCTTTTAAGCGCGACCAGCCTTCAATGCCCACCGCCTGACac                                         >  2:259924/1‑250 (MQ=255)

                                                                                                                                                                                                           agcagAAAACAGAGTAGCAGCAGAATTAACGCGGTGACCGCGCCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACAACGCCACCACGCCAAAATCACCAATCGACA‑GCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGACTGACaca                                        >  1:466197/1‑248 (MQ=255)

                                                                                                                                                                                                             cagAAAACAGAGTAGCAGCAGAATTAACGCGGTGACCGCGCCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGAAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACAACGCCACCACGCCAAAATCACCAATCGACA‑GCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGACTGACACAAc                                      >  1:443089/1‑250 (MQ=255)

                                                                                                                                                                                                              agAAAACAGAGTAGCAGCAGAATTAACGCGGTGACCGCGCCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACAACGCCACCACGCCAAAATCACCAATCGACA‑GCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCCCTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGACTgccccaccct                                    >  1:598563/1‑242 (MQ=255)

                                                                                                                                                                                                                  aaCAGAGTAGCAGCAGAATTAACGCGGTGACCGCGCCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACAACGCCACCACGCCAAAATCACCAATCGACA‑GCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGACTGACACAACATACTg                                <  1:218672/251‑1 (MQ=255)

                                                                                                                                                                                                                   aCAGAGTAGCAGCAGAATTAACGCGGTGACCGCGCCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAAGCATCGTTACCGAACAACGCCACCACGCCAAAATCACCAATCGACA‑GCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGACTGACACAACATACTGt                               <  2:574096/251‑1 (MQ=255)

                                                                                                                                                                                                                     agagTAGCAGCAGAATTAACGCGGTGACCGCGCCGTCCTGGCTGCGATAGGAGCCAATTGGCTGGTAGAGATAAAACGGCAGTGTGCGGAAATCATCGTTACCGAACAACGCCACCACGCCAAAATCTCCAATCGACA‑GCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGTACGTTTCAGGGCGCGCTGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGACTGACACAACATACTGTAg                             <  1:77748/251‑1 (MQ=255)

                                                                                                                                                                                                                           gcagcagAATTAACGCGGTGACCGCGCCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACAACGCCACCACGCCAAAATCACCAATCGACA‑GCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGACTGACACAACATACTGTAgcgggcg                       <  1:231233/251‑1 (MQ=255)

                                                                                                                                                                                                                                      aaCGCGGTGACCGCGCCGTCCTTGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACCACGCCACCACGCCAAAATCACCAATCGACA‑GCACGCAGGCAAAAGCCAGCGCCTGCCCCAGTGGGCGTTTCAGGGCGCGCATCTCGACCACTTTTAAACGCGACCAGCCTTCAATGACCAGCGACTGACACAACATACTGTAGCGGGCGGGGATATcgcg            >  2:709425/1‑251 (MQ=255)

                                                                                                                                                                                                                                          cgGTGACCGCGCCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACAACGCCACCACGCCAAAATCACCAATCGACA‑GCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGACTGACACAACATACTGTAGCGGGCGGTGATATCGCGCATc        <  1:212865/251‑1 (MQ=255)

                                                                                                                                                                                                                                              gACCGCGCCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACAACGCCACCACGCCAAAATCACCAATCGACA‑GCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGACTGACACAACATACTGTAGCGGGCGGTGATATCGCGCATCGGGt    <  1:715912/251‑1 (MQ=255)

                                                                                                                                                                                                                                               aCCGCGCCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACAACGCCACCACGCCAAAATCACCAATCGACA‑GCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGACTGACATAACATCCTGTAGCGGGCGGTGATATCGCGCATCGGGtt   <  1:245499/251‑1 (MQ=255)

                                                                                                                                                                                                                                                ccGCGCCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACAACGCCACCACGCCAAAATCACCAATCGACA‑GCACGCAGGCAAAAGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCGACCACTTTTAAGCGCGACCAGCCTTCAATGCCCAGCGACTGACACAACATACTGTAGCGGGCGGTGATATCGCGCATCGGGttt  >  1:754346/1‑251 (MQ=255)

AGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAATTCAGCAGGGTACTTTTACCCGCGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCCACCGTTAAGCTAAAACGCATCGGCAAATGGTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATTTCGCCCCGGTAGTTTTTCAATCACGGTAAACAGCAGAAAACAGAGCAGCAGCAGAATTAACGCGGTGACCGCACCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACAACGCCACCACGCCAAAATCACCAATCGACA‑GCACGCATGCAAAGGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCCACCACTTTTAAGCGTGACCAGCCTTCAATCCCCAGCGACTGACATAACATGCTGTAGCGGGCGGTGATATCGCGCATCGGGTTT  >  NC_000913/72757‑73247

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`